Rroxscaffold_3G00275880

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007625
Physical Location & Seq
Forward (+)
67190917 .. 67194908
3992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275880.1

Sequence Viewer

Length: 2370 bp
ATGGCTTCCATCCCGGCCTTGAAGCGCTCCGATTCAATTGCAGACACTATGCCAGATGCTCTGAGACAGAGCCGCTATCACATGAAGAAATGTTTTGCCAGGTTTGTCGGGAATGGGAAGAGGTTAATGAAGCCCCAGCATATAATGGAGGAAATCGAAAAATCAATAGAGGACGGGAATGAAAAGAGCAAGGTGTTACGGGGATTACTTGGTTACATCCTCAGTTCCACTCAGGAAGCAGCTGTTGTTCCACCGCATGTTGCATTTTCTGTTAGACCAAATCCTGGTTTCTCAGAGTTTGTTAAGGTGAATGCTGATGATCTAGCAGTGGATGGAATCAGTGTTACCGAATACTTGAAATTCAAAGAAATGATCTACGACGAGAACTGGGCAAACGATGAAAATGCTTTGGAAATTGATTTTGGAGCCATTGACTTCTCTAGTCCTCGCATGACTCTTCCTTCTTCTATTGGGAATGGACTTAACTTCATCCTCAAGTTAATCTCCTCAAGGCTGAATGCCAGCTCATCCGATTATGCCAAACCCTTGCTTGACTACTTGTTAGCACTCACTCACCACGGACAGAATCTTATGATCAATGAAACTATGGATTCGGTGGAAAAGCTTGAAACGTCTTTGATTCGAGCACAATCGTTTGTCTCAGCACTCCCAAAAAACACACCATTTCTTAATTTCGAGCAGAGGTTAAAGGACATGGGTTTTGAGAAAGGATGGGGGGATACAGCAGAAAGAGTTGGTGAGAGAATGAGGACGCTGTCAGAAGTACTCCAAGCACCGGATTGCGTGAAGTTGGAGTCATTCTTTAGCAGCCTTCCCAATACATTCAACATTGTCATCTTCTCTCCTCACGGCTACTTTGGCCAGTCAGATGTCCTTGGATTGCCTGATACTGGTGGCCAGGTGGTTTATATACTTGATCAAGTGAGAGCCTTAGAGGAAGAGTTGCTACTGAGAATAAAGCAGCAAGGGCTTGCTGTGAAGCCTCAGATTCTTGTGGTGACGCGACTCATACCAGACTCTCGAGGGACTAAGTGCAATGTGGAGTTGGAGGCTATAGTCAACACCAAGCACTCCCACATTCTCAGGGTTCCTTTCAGGACAGACAAAGGCATTCTGCGCCATTGGGATGCAACTGCAAAGATTCTAGAACACATGGACTGCAAGCCGGACCTCATAATTGGGAACTACAGTGATGGAAACTTGGTGGCCTCTTTGATGGCTGCCAAGCTTGGAATAACACAGGGAACAATTGCCCACGCTCTAGAAAAGACCAAGTATGAAGACTCGGATGCCAAATGGAAGGAATTTGATACAAAGTACCACTTTTCTTGTCAGTTCACTGCAGACTTAATCTCGATGAACTCAACTGATTTTATCATAACTAGCACATTTCAAGAAATAGCAGGAGGAGGATTTTTGGCAGACAAAAAGAAACCAATCATCTTTTCAATGGCAAGACTGGATAAAGTGAAGAACCTCTCAGGACTAGTTGATTGGTTCGGAAAGAACAAGAGGCTGAGGAATTTGGTCAACCTTGTTATATTCAGATGGATAGCAGCTCAAACTGATAGGTATCGCAACGGAGAGTTGTACCGCTGCATTGCTGATACAAAGGGAGCTTTTGTGCAGCCTGCATTGTATGAAGCCTTCGGTCTTACAGTGATTGAGGCAATGAACTGTGGATTGCCCTCTTTTGCTACAAACCAAGGAGGCCCTGCTGAAATTATCGTTGATGGAGTCTCGGGCTTCCATATTGATCCCAACAATGGTGAAGAGTCCAGCAACAAGATTGCTGATTTCTTTGAGAGATGCAGGGCAGATGGTGAATACTGGAAGAAGATGTCCGCGGCGGGTCTGCAGCGTATATATGCATGCTATACATGGAAAATATATGCAAACAAAATGTTGAACATGGCATCCACTTATGGGTTTTGGAGGCAGTTGAGGAAGGAGCCGAAGCTAGCAAAGCAAAGCTACATTCATATGTTTTACAGTCTCCTATTTAGGACTTTGGCGAGGACTGTCCCTGTCCCAAGTGATGGATATGAACCAGTACCGATGCCAAAGGCAATAGCTGCAGCTGACCAAGGCAAACCAAAGTCAGTGACAACTGTATCCAAACCCCCACAAGCCGCAGCTGCACCAACAATGCCAACAGTTCCAGTTGCTCAGATCACACCAAGACCAAGGTATGAAGGAGGTTTAGAGCAGATAAATGACCAACCTATTGGCCCTCCAGATGTTGCAAGTCCAAACCGCCCAACACGGTGCCTTTGGAATTGCTTCTTCGTCATCATTGGTTTTCTGTGCCTTGTTTATTACAAGCTCAAGAATATGTACTACAAATGA

Protein Analysis

789

Amino Acids

88.42

Weight (kDa)

8.47

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 9 - 123 4.7e-46 Sucrose synthase N-terminal
SUS_EPBD PF24862 159 - 247 1.1e-26 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 270 - 477 1e-109 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 543 - 609 1.1e-17 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 547 - 607 2e-11 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2289
AccB7I CCANNNNNTGG 2 cut(s) 284, 2060
AccII CGCG 2 cut(s) 1024, 1868
AciI CCGC 8 cut(s) 73, 254, 1615, 1866, 1868, 1871, 2154, 2278
AclWI GGATC 1 cut(s) 1772
AcoI YGGCCR 2 cut(s) 880, 916
AcsI RAATTY 3 cut(s) 359, 1325, 1543
AfaI GTAC 5 cut(s) 786, 1340, 1613, 2076, 2360
AfeI AGCGCT 1 cut(s) 26
AfiI CCNNNNNNNGG 6 cut(s) 284, 796, 911, 1787, 1947, 2060
AgsI TTSAA 9 cut(s) 22, 36, 358, 364, 629, 847, 1415, 1470, 1930
AhlI ACTAGT 1 cut(s) 1507
AjnI CCWGG 3 cut(s) 98, 283, 918
AjuI GAANNNNNNNTTGG 4 cut(s) 405, 437, 1450, 1482
AloI GAACNNNNNNTCC 2 cut(s) 1922, 1954
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 4 cut(s) 58, 664, 1765, 2021
AlwI GGATC 1 cut(s) 1772
Ama87I CYCGRG 2 cut(s) 1041, 1762
Aor51HI AGCGCT 1 cut(s) 26
AoxI GGCC 6 cut(s) 15, 880, 916, 1227, 1732, 2251
ApoI RAATTY 3 cut(s) 359, 1325, 1543
ArsI GACNNNNNNTTYG 2 cut(s) 704, 736
Asp700I GAANNNNTTC 1 cut(s) 2303
AspLEI GCGC 2 cut(s) 27, 1140
AspS9I GGNCC 3 cut(s) 1189, 1733, 2252
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 6 cut(s) 319, 566, 770, 1030, 1802, 1856
AsuNHI GCTAGC 1 cut(s) 1981
AvaI CYCGRG 2 cut(s) 1041, 1762
AvaII GGWCC 1 cut(s) 1189
BaeI ACNNNNGTAYC 2 cut(s) 2118, 2151
BalI TGGCCA 2 cut(s) 882, 918
BanI GGYRCC 1 cut(s) 2289
BarI GAAGNNNNNNTAC 4 cut(s) 951, 983, 1652, 1684
BbsI GAAGAC 1 cut(s) 1308
Bbv12I GWGCWC 1 cut(s) 649
BbvCI CCTCAGC 1 cut(s) 1538
BccI CCATC 9 cut(s) 17, 326, 726, 1208, 1231, 1563, 1748, 1835, 2054
BceAI ACGGC 1 cut(s) 886
BcgI CGANNNNNNTGC 4 cut(s) 20, 54, 386, 420
BciT130I CCWGG 3 cut(s) 100, 285, 920
BciVI GTATCC 2 cut(s) 733, 2146
BclI TGATCA 2 cut(s) 594, 937
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 4 cut(s) 58, 664, 1765, 2021
BcuI ACTAGT 1 cut(s) 1507
BfaI CTAG 7 cut(s) 323, 441, 1166, 1283, 1404, 1508, 1982
BfmI CTRYAG 5 cut(s) 1074, 1207, 1362, 1877, 2097
BfoI RGCGCY 1 cut(s) 28
BfuI GTATCC 2 cut(s) 733, 2146
BmcAI AGTACT 1 cut(s) 786
Bme1390I CCNGG 4 cut(s) 14, 100, 285, 920
Bme18I GGWCC 1 cut(s) 1189
BmeT110I CYCGRG 2 cut(s) 1041, 1762
BmgT120I GGNCC 3 cut(s) 1189, 1733, 2252
BmiI GGNNCC 4 cut(s) 427, 1110, 1974, 2291
BmrFI CCNGG 4 cut(s) 14, 100, 285, 920
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 6 cut(s) 46, 1138, 1300, 1820, 1946, 2070
BmtI GCTAGC 1 cut(s) 1985
BmuI ACTGGG 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 1308
BpmI CTGGAG 1 cut(s) 2241
Bpu10I CCTNAGC 1 cut(s) 1538
BpuEI CTTGAG 3 cut(s) 479, 493, 2333
BpuMI CCSGG 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 1593
BsaJI CCNNGG 6 cut(s) 577, 895, 1726, 1866, 2107, 2207
BsaWI WCCGGW 1 cut(s) 796
BsaXI ACNNNNNCTCC 2 cut(s) 1629, 1659
Bsc4I CCNNNNNNNGG 6 cut(s) 284, 796, 911, 1787, 1947, 2060
Bse1I ACTGG 7 cut(s) 392, 883, 916, 1485, 1856, 2072, 2183
Bse3DI GCAATG 3 cut(s) 1063, 1620, 1698
Bse8I GATNNNNATC 1 cut(s) 1593
BseBI CCWGG 3 cut(s) 100, 285, 920
BseDI CCNNGG 6 cut(s) 577, 895, 1726, 1866, 2107, 2207
BseGI GGATG 9 cut(s) 9, 216, 337, 489, 527, 737, 1153, 1315, 1937
BseJI GATNNNNATC 1 cut(s) 1593
BseLI CCNNNNNNNGG 6 cut(s) 284, 796, 911, 1787, 1947, 2060
BseMI GCAATG 3 cut(s) 1063, 1620, 1698
BseNI ACTGG 7 cut(s) 392, 883, 916, 1485, 1856, 2072, 2183
BseRI GAGGAG 3 cut(s) 496, 855, 1443
BseYI CCCAGC 1 cut(s) 135
BsgI GTGCAG 2 cut(s) 1667, 2145
Bsh1236I CGCG 2 cut(s) 1024, 1868
BshFI GGCC 6 cut(s) 17, 882, 918, 1229, 1734, 2253
BshNI GGYRCC 1 cut(s) 2289
BsiHKAI GWGCWC 1 cut(s) 649
BsiHKCI CYCGRG 2 cut(s) 1041, 1762
BsiSI CCGG 3 cut(s) 14, 797, 1187
BslFI GGGAC 3 cut(s) 1060, 2030, 2036
BslI CCNNNNNNNGG 6 cut(s) 284, 796, 911, 1787, 1947, 2060
BsmAI GTCTC 4 cut(s) 58, 664, 1765, 2021
BsmFI GGGAC 3 cut(s) 1060, 2030, 2036
BsmI GAATGC 3 cut(s) 316, 523, 1131
BsnI GGCC 6 cut(s) 17, 882, 918, 1229, 1734, 2253
BsoBI CYCGRG 2 cut(s) 1041, 1762
Bsp1286I GDGCHC 1 cut(s) 649
Bsp143I GATC 6 cut(s) 319, 372, 594, 937, 1777, 2193
BspACI CCGC 8 cut(s) 73, 254, 1615, 1866, 1868, 1871, 2154, 2278
BspANI GGCC 6 cut(s) 17, 882, 918, 1229, 1734, 2253
BspFNI CGCG 2 cut(s) 1024, 1868
BspLI GGNNCC 4 cut(s) 427, 1110, 1974, 2291
BspMAI CTGCAG 3 cut(s) 1366, 1881, 2101
BspOI GCTAGC 1 cut(s) 1985
BspPI GGATC 1 cut(s) 1772
BspT107I GGYRCC 1 cut(s) 2289
BsrDI GCAATG 3 cut(s) 1063, 1620, 1698
BsrI ACTGG 7 cut(s) 392, 883, 916, 1485, 1856, 2072, 2183
BssECI CCNNGG 6 cut(s) 577, 895, 1726, 1866, 2107, 2207
BssMI GATC 6 cut(s) 319, 372, 594, 937, 1777, 2193
BssT1I CCWWGG 4 cut(s) 895, 1726, 2107, 2207
Bst2UI CCWGG 3 cut(s) 100, 285, 920
Bst4CI ACNGT 8 cut(s) 1211, 1681, 1700, 2015, 2044, 2134, 2179, 2289
Bst6I CTCTTC 4 cut(s) 113, 462, 954, 1788
BstAPI GCANNNNNTGC 1 cut(s) 2096
BstC8I GCNNGC 6 cut(s) 523, 993, 1184, 1653, 1894, 1983
BstDSI CCRYGG 2 cut(s) 577, 1866
BstF5I GGATG 9 cut(s) 9, 216, 337, 489, 527, 737, 1153, 1315, 1937
BstFNI CGCG 2 cut(s) 1024, 1868
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 2 cut(s) 27, 1140
BstKTI GATC 6 cut(s) 322, 375, 597, 940, 1780, 2196
BstMAI GTCTC 4 cut(s) 58, 664, 1765, 2021
BstMBI GATC 6 cut(s) 319, 372, 594, 937, 1777, 2193
BstMWI GCNNNNNNNGC 6 cut(s) 879, 988, 1137, 1987, 2096, 2159
BstNI CCWGG 3 cut(s) 100, 285, 920
BstNSI RCATGY 2 cut(s) 260, 1896
BstSCI CCNGG 4 cut(s) 12, 98, 283, 918
BstSFI CTRYAG 5 cut(s) 1074, 1207, 1362, 1877, 2097
BstUI CGCG 2 cut(s) 1024, 1868
BstV2I GAAGAC 1 cut(s) 1308
BstXI CCANNNNNNTGG 1 cut(s) 2249
BsuI GTATCC 2 cut(s) 733, 2146
BsuRI GGCC 6 cut(s) 17, 882, 918, 1229, 1734, 2253
BtgI CCRYGG 2 cut(s) 577, 1866
BtsCI GGATG 9 cut(s) 9, 216, 337, 489, 527, 737, 1153, 1315, 1937
BtsI GCAGTG 2 cut(s) 333, 1359
BtsIMutI CAGTG 6 cut(s) 333, 346, 1216, 1359, 1686, 2130
Cac8I GCNNGC 6 cut(s) 523, 993, 1184, 1653, 1894, 1983
CfoI GCGC 2 cut(s) 27, 1140
Cfr13I GGNCC 3 cut(s) 1189, 1733, 2252
Cfr42I CCGCGG 1 cut(s) 1869
CseI GACGC 2 cut(s) 781, 1030
Csp6I GTAC 5 cut(s) 785, 1339, 1612, 2075, 2359
CspCI CAANNNNNGTGG 2 cut(s) 1331, 1366
CviAII CATG 8 cut(s) 82, 257, 451, 715, 1174, 1893, 1902, 1933
CviQI GTAC 5 cut(s) 785, 1339, 1612, 2075, 2359
DpnI GATC 6 cut(s) 321, 374, 596, 939, 1779, 2195
DpnII GATC 6 cut(s) 319, 372, 594, 937, 1777, 2193
EaeI YGGCCR 2 cut(s) 880, 916
Eam1104I CTCTTC 4 cut(s) 113, 462, 954, 1788
EarI CTCTTC 4 cut(s) 113, 462, 954, 1788
Eco130I CCWWGG 4 cut(s) 895, 1726, 2107, 2207
Eco47I GGWCC 1 cut(s) 1189
Eco47III AGCGCT 1 cut(s) 26
Eco88I CYCGRG 2 cut(s) 1041, 1762
EcoO109I RGGNCCY 1 cut(s) 1733
EcoRII CCWGG 3 cut(s) 98, 283, 918
EcoT14I CCWWGG 4 cut(s) 895, 1726, 2107, 2207
EcoT22I ATGCAT 1 cut(s) 1894
ErhI CCWWGG 4 cut(s) 895, 1726, 2107, 2207
FaeI CATG 8 cut(s) 85, 260, 454, 718, 1177, 1896, 1905, 1936
FalI AAGNNNNNCTT 2 cut(s) 1328, 1360
FaqI GGGAC 3 cut(s) 1060, 2030, 2036
FatI CATG 8 cut(s) 81, 256, 450, 714, 1173, 1892, 1901, 1932
FauI CCCGC 1 cut(s) 1864
FauNDI CATATG 1 cut(s) 2004
FbaI TGATCA 2 cut(s) 594, 937
FokI GGATG 8 cut(s) 203, 344, 476, 514, 744, 1160, 1322, 1924
FspBI CTAG 7 cut(s) 323, 441, 1166, 1283, 1404, 1508, 1982
GlaI GCGC 2 cut(s) 26, 1139
GsaI CCCAGC 1 cut(s) 139
GsuI CTGGAG 1 cut(s) 2241
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 6 cut(s) 17, 882, 918, 1229, 1734, 2253
HapII CCGG 3 cut(s) 14, 797, 1187
HgaI GACGC 2 cut(s) 781, 1030
HhaI GCGC 2 cut(s) 27, 1140
Hin1II CATG 8 cut(s) 85, 260, 454, 718, 1177, 1896, 1905, 1936
Hin6I GCGC 2 cut(s) 25, 1138
HinP1I GCGC 2 cut(s) 25, 1138
HincII GTYRAC 2 cut(s) 1081, 1552
HindII GTYRAC 2 cut(s) 1081, 1552
HindIII AAGCTT 2 cut(s) 623, 1247
HpaII CCGG 3 cut(s) 14, 797, 1187
HphI GGTGA 6 cut(s) 319, 566, 770, 1030, 1802, 1856
Hpy166II GTNNAC 3 cut(s) 1081, 1359, 1552
Hpy8I GTNNAC 3 cut(s) 1081, 1359, 1552
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 6 cut(s) 471, 842, 1315, 1678, 1963, 2210
HpyCH4III ACNGT 8 cut(s) 1211, 1681, 1700, 2015, 2044, 2134, 2179, 2289
HpyCH4IV ACGT 1 cut(s) 632
HpyF10VI GCNNNNNNNGC 6 cut(s) 879, 988, 1137, 1987, 2096, 2159
HpySE526I ACGT 1 cut(s) 632
Hsp92II CATG 8 cut(s) 85, 260, 454, 718, 1177, 1896, 1905, 1936
HspAI GCGC 2 cut(s) 25, 1138
Ksp22I TGATCA 2 cut(s) 594, 937
KspI CCGCGG 1 cut(s) 1869
Kzo9I GATC 6 cut(s) 319, 372, 594, 937, 1777, 2193
LmnI GCTCC 4 cut(s) 32, 425, 1637, 1972
LweI GCATC 6 cut(s) 46, 1138, 1300, 1820, 1946, 2070
MaeI CTAG 7 cut(s) 323, 441, 1166, 1283, 1404, 1508, 1982
MaeII ACGT 1 cut(s) 632
MaeIII GTNAC 5 cut(s) 195, 212, 343, 1018, 2125
MalI GATC 6 cut(s) 321, 374, 596, 939, 1779, 2195
MboI GATC 6 cut(s) 319, 372, 594, 937, 1777, 2193
MfeI CAATTG 2 cut(s) 36, 1269
MhlI GDGCHC 1 cut(s) 649
MlsI TGGCCA 2 cut(s) 882, 918
MluNI TGGCCA 2 cut(s) 882, 918
MlyI GAGTC 7 cut(s) 448, 824, 1020, 1031, 1298, 1767, 1805
MmeI TCCRAC 2 cut(s) 792, 1047
Mox20I TGGCCA 2 cut(s) 882, 918
Mph1103I ATGCAT 1 cut(s) 1894
MroXI GAANNNNTTC 1 cut(s) 2303
MscI TGGCCA 2 cut(s) 882, 918
MseI TTAA 7 cut(s) 125, 303, 483, 500, 690, 707, 1370
MslI CAYNNNNRTG 2 cut(s) 1146, 2003
Msp20I TGGCCA 2 cut(s) 882, 918
MspA1I CMGCKG 5 cut(s) 242, 1617, 1868, 2102, 2159
MspI CCGG 3 cut(s) 14, 797, 1187
MspR9I CCNGG 4 cut(s) 14, 100, 285, 920
MunI CAATTG 2 cut(s) 36, 1269
Mva1269I GAATGC 3 cut(s) 316, 523, 1131
MvaI CCWGG 3 cut(s) 100, 285, 920
MvnI CGCG 2 cut(s) 1024, 1868
MwoI GCNNNNNNNGC 6 cut(s) 879, 988, 1137, 1987, 2096, 2159
NciI CCSGG 1 cut(s) 14
NdeI CATATG 1 cut(s) 2004
NdeII GATC 6 cut(s) 319, 372, 594, 937, 1777, 2193
NheI GCTAGC 1 cut(s) 1981
NlaIII CATG 8 cut(s) 85, 260, 454, 718, 1177, 1896, 1905, 1936
NlaIV GGNNCC 4 cut(s) 427, 1110, 1974, 2291
NmuCI GTSAC 2 cut(s) 1018, 2125
NsiI ATGCAT 1 cut(s) 1894
NspI RCATGY 2 cut(s) 260, 1896
PaeI GCATGC 1 cut(s) 1896
PaeR7I CTCGAG 1 cut(s) 1041
PctI GAATGC 3 cut(s) 316, 523, 1131
PdmI GAANNNNTTC 1 cut(s) 2303
PfeI GAWTC 7 cut(s) 32, 336, 586, 611, 640, 1009, 1162
PflFI GACNNNGTC 1 cut(s) 775
PflMI CCANNNNNTGG 2 cut(s) 284, 2060
PleI GAGTC 7 cut(s) 448, 823, 1020, 1031, 1298, 1766, 1804
PpsI GAGTC 7 cut(s) 448, 823, 1020, 1031, 1298, 1766, 1804
Psp6I CCWGG 3 cut(s) 98, 283, 918
PspFI CCCAGC 1 cut(s) 135
PspGI CCWGG 3 cut(s) 98, 283, 918
PspN4I GGNNCC 4 cut(s) 427, 1110, 1974, 2291
PspPI GGNCC 3 cut(s) 1189, 1733, 2252
PstI CTGCAG 3 cut(s) 1366, 1881, 2101
PsyI GACNNNGTC 1 cut(s) 775
PvuII CAGCTG 3 cut(s) 242, 2102, 2159
RsaI GTAC 5 cut(s) 786, 1340, 1613, 2076, 2360
RsaNI GTAC 5 cut(s) 785, 1339, 1612, 2075, 2359
RseI CAYNNNNRTG 2 cut(s) 1146, 2003
SacII CCGCGG 1 cut(s) 1869
SaqAI TTAA 7 cut(s) 125, 303, 483, 500, 690, 707, 1370
Sau3AI GATC 6 cut(s) 319, 372, 594, 937, 1777, 2193
Sau96I GGNCC 3 cut(s) 1189, 1733, 2252
ScaI AGTACT 1 cut(s) 786
SchI GAGTC 7 cut(s) 448, 824, 1020, 1031, 1298, 1767, 1805
ScrFI CCNGG 4 cut(s) 14, 100, 285, 920
SduI GDGCHC 1 cut(s) 649
SfaNI GCATC 6 cut(s) 46, 1138, 1300, 1820, 1946, 2070
SfcI CTRYAG 5 cut(s) 1074, 1207, 1362, 1877, 2097
Sfr274I CTCGAG 1 cut(s) 1041
Sfr303I CCGCGG 1 cut(s) 1869
SgrBI CCGCGG 1 cut(s) 1869
SinI GGWCC 1 cut(s) 1189
SlaI CTCGAG 1 cut(s) 1041
SmiMI CAYNNNNRTG 2 cut(s) 1146, 2003
SmlI CTYRAG 4 cut(s) 494, 508, 1041, 2348
SmoI CTYRAG 4 cut(s) 494, 508, 1041, 2348
SpeI ACTAGT 1 cut(s) 1507
SphI GCATGC 1 cut(s) 1896
SsiI CCGC 8 cut(s) 73, 254, 1615, 1866, 1868, 1871, 2154, 2278
SspMI CTAG 7 cut(s) 323, 441, 1166, 1283, 1404, 1508, 1982
StyD4I CCNGG 4 cut(s) 12, 98, 283, 918
StyI CCWWGG 4 cut(s) 895, 1726, 2107, 2207
TaaI ACNGT 8 cut(s) 1211, 1681, 1700, 2015, 2044, 2134, 2179, 2289
TaiI ACGT 1 cut(s) 635
TaqI TCGA 5 cut(s) 156, 643, 696, 1042, 1376
TaqII GACCGA 1 cut(s) 1661
TatI WGTACW 2 cut(s) 784, 2358
TauI GCSGC 3 cut(s) 75, 1871, 2156
TfiI GAWTC 7 cut(s) 32, 336, 586, 611, 640, 1009, 1162
Tru1I TTAA 7 cut(s) 125, 303, 483, 500, 690, 707, 1370
Tru9I TTAA 7 cut(s) 125, 303, 483, 500, 690, 707, 1370
TscAI CASTG 6 cut(s) 333, 346, 1216, 1366, 1686, 2130
TseFI GTSAC 2 cut(s) 1018, 2125
Tsp45I GTSAC 2 cut(s) 1018, 2125
TspGWI ACGGA 2 cut(s) 594, 1617
TspRI CASTG 6 cut(s) 333, 346, 1216, 1366, 1686, 2130
Tth111I GACNNNGTC 1 cut(s) 775
Van91I CCANNNNNTGG 2 cut(s) 284, 2060
VpaK11BI GGWCC 1 cut(s) 1189
XapI RAATTY 3 cut(s) 359, 1325, 1543
XbaI TCTAGA 2 cut(s) 1165, 1282
XceI RCATGY 2 cut(s) 260, 1896
XhoI CTCGAG 1 cut(s) 1041
XmnI GAANNNNTTC 1 cut(s) 2303
XspI CTAG 7 cut(s) 323, 441, 1166, 1283, 1404, 1508, 1982
ZrmI AGTACT 1 cut(s) 786
Zsp2I ATGCAT 1 cut(s) 1894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.