Rw7G000450

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007625
Physical Location & Seq
Reverse (-)
378375 .. 382193
3819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000450.1

Sequence Viewer

Length: 2763 bp
ATGGCTTCCATCCCGGCCTTGAAGCGCTCCGATTCAATTGCAGACACTATGCCAGATGCTCTGAGACAGAGCCGCTATCACATGAAGAAATGTTTTGCCAGGTTTGTCGGGAATGGGAAGAGGTTAATGAAGCCCCAGCATATAATGGAGGAAATCGAAAAATCAATAGGGGACGGGAATGAAAAAAGCAAGGTGTTACGGGGATTACTTGGTTACATCCTCAGTTCCACTCAGGAAGCAGCTGTTGTTCCGCCGCATGTTGCATTTTCTGTTAGACCAAATCCTGGTTTCTCAGAGTTTGTTAAGGTGAATGCTGATGATCTAGCAGTGGACGGAATCAGTGTTACCGAATACTTGAAATTCAAAGAAATGATCTACGACGAGAACTGGGCAAATGATGAAAATGCTTTGGAAATTGATTTTGGAGCCATTGACTTCTCTAGTCCTCGCATGACCCTTCCTTCTTCTATTGGGAATGGACTTAACTTCATCCTCAAGTTAATCTCCTCGAGGCTGAATGCCAGCTCATCCGATTATGCCAAACCCTTGCTTGACTACTTGTTAGCACTCAATCACCACGGACAGAATCTTATGATCAATGAAACTATGGATTCGGTGGAAAAGCTTGAAACGTCATTGATTCGAGCACAATCGTTTGTCTCAGCACTCCCAAAAAACACACCATTTCTTAATTTCGAGCAGAGGTACTCATCCATCTTTCACTTTTGTGCTGATCCTTGTATTGCGGGTAAGTACATTGTGGTGAATTTATTAATTTTCAGGTTAAAGGATATGGGTTTAGAGAAAGGATGGGGGGATACAGCAGAAAGAGTTGTTGAGAGAATGAGGACACTGTCAGAAGTACTCCAAGCACCGGATTGCGTGAAGTTGGAGTCATTCTTTAGCAGCCTTCCCAATACATTCAACATTGTCATCTTCTCTCCTCACGGCTACTTTGGCCAGTCAGATGTCCTTGGATTGCCTGATACTGGTGGCCAGGTGGTTTATATACTTGATCAAGTGAGAGCCTTAGAGGAAGAGTTGCTGATGAGAATAAAGCAGCAAGGGCTTGCTGTGAAGCCTCAGATTCTTGTGGTGACGCGACTCATACCAGACTCTCGAGGGACTAAGTGCAATGTGGAGTTGGAGGCTATAGTCAACACTAAGCACTCCCACATTCTCAGGGTTCCTTTCAGGACAGACAAAGGCATTCTGCGCCATTGGGTGTCCCGTTTTGATATCTACCCTTATCTTGAGACTTTTGTCCAGGATGCAACTGCAAAGATTCTAGAGCACATGGACTGCAAACCGGACCTCATAATTGGGAACTACAGTGATGGAAACTTGGTGGCCTCTTTGATGGCTGCCAAGCTTGGAATAACACAGGGAACAATTGCCCACGCTCTAGAAAAGACCAAGTATGAAGACTCGGATGCCAAATGGAAGGAATTTGATACAAAGTACCACTTTTCTTGTCAGTTCACTGCAGACTTAATCTCGATGAACTCAACTGATTTTGTCATAACTAGCACATTTCAAGAAATAGCAGGAGGTAAGGATCGACCTGGACAGTATGAAAGCCACACCGCATTTACAATGCCAGGACTGTGTCGAGTAGTCTCAGGCATTGACGTGTTTGATCCGAAGTTCAACATTGCTGCTCCCGGTGCTGACCAATCTGTCTACTTCCCCAACTCCGAAAAACAGAAGCGTTTTACCAAATTTCATCCTTCCATTGAACAAATGGTCTACAGTAAGGAGGAAAACGACGAGCACATAGGATTTTTGGCAGACAAAAAGAAACCAATCATCTTTTCAATGGCAAGACTGGATAAAGTGAAGAACCTCTCAGGACTAGTTGAGTGGTTCGGAAAGAACAAGAGGCTGAGGAATTTGGTCAACCTTGTTATAGTAGGGGGGTTCTTTGACCCATCGAAGTCCAAAGACAGAGAAGAAATTGCTGAAATTATCAAACTGCATGCCTTGGTTAAAGAATATCAACTTAATGGCCAGTTCAGATGGATAGCAGCTCAAACTGATAGGTATCGCAACGGAGAGTTGTACCGCTGCATTGCTGATACAAAGGGAGCTTTTGTGCAGCCTGCATTGTATGAAGCCTTTGGTCTTACAGTGATTGAGGCAATGAACTGTGGATTGCCCTCTTTTGCTACAAACCAAGGAGGCCCGGCTGAAATTATTGTTGATGGAGTCTCGGGCTTCCATATTGATCCCAACAATGGTGAAGAGTCCAGCAACAAGATTGCTGATTTCTTTGAGAGATGCAGGGCAGATGGTGAATACTGGAAGAAGATCTCCGCGGCGGGTCTGCAGCGTATATACGCATGCTATACATGGAAAATATATGCAAACAAAATGTTGAACATGGGATCCACTTATGGGTTTTGGAGGCAGTTGAGGAAGGAGCCGAAGCTAGCAAAGCAAAGCTACATTCATATGTTTTACAGTCTCCTATTTAGGACTTTGGCAAGGACTGTCCCTGTCCCAAGTGATGGATATGAACCAGTACCGATGCCAAAGGCAATAGCTGCAGCTGACCAAGGCAAACCAAAGTCAGTGACAACTGTATACAAACCCCCACAAGCCGCAGCTGCACCAACAATGCCAACAGTTTCACAACCTATTGGCCCTCCTGATGTTGCAAGTCCAAACCGCCCAACACGGTGCCTTTGGAATTGCTTCTTCGTCATCATTGGTTTCCTGTGCCTTGTTTATTACAAGCTCAAGAATATGTACTACAAATGA

Protein Analysis

920

Amino Acids

103.54

Weight (kDa)

8.27

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 9 - 123 2.1e-45 Sucrose synthase N-terminal
SUS_EPBD PF24862 159 - 236 1e-17 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 296 - 584 1e-167 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 595 - 759 2e-29 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 602 - 757 1.1e-13 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1679
AbsI CCTCGAGG 1 cut(s) 508
AccB1I GGYRCC 1 cut(s) 2682
AccB7I CCANNNNNTGG 2 cut(s) 284, 2510
AccI GTMKAC 3 cut(s) 1683, 1749, 2586
AccII CGCG 2 cut(s) 1102, 2318
AclWI GGATC 6 cut(s) 728, 1566, 1634, 2222, 2382, 2395
AcoI YGGCCR 3 cut(s) 958, 994, 2008
AcsI RAATTY 5 cut(s) 359, 766, 1448, 1721, 1891
AfaI GTAC 7 cut(s) 707, 755, 864, 1463, 2063, 2526, 2753
AfeI AGCGCT 1 cut(s) 26
AfiI CCNNNNNNNGG 6 cut(s) 284, 874, 989, 2237, 2397, 2510
AflIII ACRYGT 1 cut(s) 1632
AhlI ACTAGT 1 cut(s) 1855
AjiI CACGTC 1 cut(s) 1633
AjnI CCWGG 6 cut(s) 98, 283, 996, 1266, 1564, 1600
AjuI GAANNNNNNNTTGG 4 cut(s) 405, 437, 1798, 1830
AleI CACNNNNGTG 1 cut(s) 726
AloI GAACNNNNNNTCC 2 cut(s) 2372, 2404
Alw21I GWGCWC 3 cut(s) 649, 1296, 1776
Alw26I GTCTC 6 cut(s) 58, 664, 1250, 1624, 2215, 2471
AlwI GGATC 6 cut(s) 728, 1566, 1634, 2222, 2382, 2395
Ama87I CYCGRG 3 cut(s) 508, 1119, 2212
Aor51HI AGCGCT 1 cut(s) 26
AoxI GGCC 7 cut(s) 15, 958, 994, 1350, 2008, 2182, 2644
ApoI RAATTY 5 cut(s) 359, 766, 1448, 1721, 1891
AseI ATTAAT 1 cut(s) 773
Asp700I GAANNNNTTC 1 cut(s) 2696
AspLEI GCGC 2 cut(s) 27, 1218
AspS9I GGNCC 3 cut(s) 1312, 2183, 2645
AsuC2I CCSGG 3 cut(s) 14, 1665, 2186
AsuHPI GGTGA 6 cut(s) 319, 566, 775, 1108, 2252, 2306
AsuNHI GCTAGC 1 cut(s) 2431
AvaI CYCGRG 3 cut(s) 508, 1119, 2212
AvaII GGWCC 1 cut(s) 1312
BalI TGGCCA 3 cut(s) 960, 996, 2010
BamHI GGATCC 1 cut(s) 2387
BanI GGYRCC 1 cut(s) 2682
BbsI GAAGAC 1 cut(s) 1431
Bbv12I GWGCWC 3 cut(s) 649, 1296, 1776
BbvCI CCTCAGC 1 cut(s) 1886
BceAI ACGGC 1 cut(s) 964
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 6 cut(s) 100, 285, 998, 1268, 1566, 1602
BciVI GTATCC 1 cut(s) 811
BclI TGATCA 2 cut(s) 594, 1015
BcnI CCSGG 3 cut(s) 14, 1665, 2186
BcoDI GTCTC 6 cut(s) 58, 664, 1250, 1624, 2215, 2471
BcuI ACTAGT 1 cut(s) 1855
BfaI CTAG 7 cut(s) 323, 441, 1289, 1406, 1527, 1856, 2432
BfmI CTRYAG 6 cut(s) 1152, 1330, 1485, 1750, 2327, 2547
BfoI RGCGCY 1 cut(s) 28
BfuI GTATCC 1 cut(s) 811
BglII AGATCT 1 cut(s) 2310
BmcAI AGTACT 1 cut(s) 864
Bme1390I CCNGG 9 cut(s) 14, 100, 285, 998, 1268, 1566, 1602, 1665, 2186
Bme18I GGWCC 1 cut(s) 1312
BmeT110I CYCGRG 3 cut(s) 508, 1119, 2212
BmgBI CACGTC 1 cut(s) 1633
BmgT120I GGNCC 3 cut(s) 1312, 2183, 2645
BmiI GGNNCC 5 cut(s) 427, 1188, 2389, 2424, 2684
BmrFI CCNGG 9 cut(s) 14, 100, 285, 998, 1268, 1566, 1602, 1665, 2186
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 5 cut(s) 46, 1261, 1423, 2270, 2520
BmtI GCTAGC 1 cut(s) 2435
BmuI ACTGGG 1 cut(s) 397
BoxI GACNNNNGTC 1 cut(s) 1262
BpiI GAAGAC 1 cut(s) 1431
Bpu10I CCTNAGC 1 cut(s) 1886
BpuEI CTTGAG 3 cut(s) 479, 1274, 2726
BpuMI CCSGG 3 cut(s) 14, 1665, 2186
BsaBI GATNNNNATC 1 cut(s) 2043
BsaJI CCNNGG 6 cut(s) 577, 973, 1983, 2176, 2316, 2557
BsaWI WCCGGW 2 cut(s) 874, 1309
BsaXI ACNNNNNCTCC 2 cut(s) 2079, 2109
Bsc4I CCNNNNNNNGG 6 cut(s) 284, 874, 989, 2237, 2397, 2510
Bse1I ACTGG 7 cut(s) 392, 961, 994, 1833, 2011, 2306, 2522
Bse3DI GCAATG 4 cut(s) 1141, 1653, 2070, 2148
Bse8I GATNNNNATC 1 cut(s) 2043
BseBI CCWGG 6 cut(s) 100, 285, 998, 1268, 1566, 1602
BseDI CCNNGG 6 cut(s) 577, 973, 1983, 2176, 2316, 2557
BseGI GGATG 9 cut(s) 9, 216, 489, 527, 710, 815, 1276, 1438, 1726
BseJI GATNNNNATC 1 cut(s) 2043
BseLI CCNNNNNNNGG 6 cut(s) 284, 874, 989, 2237, 2397, 2510
BseMI GCAATG 4 cut(s) 1141, 1653, 2070, 2148
BseNI ACTGG 7 cut(s) 392, 961, 994, 1833, 2011, 2306, 2522
BseRI GAGGAG 2 cut(s) 496, 933
BseYI CCCAGC 1 cut(s) 135
BsgI GTGCAG 2 cut(s) 2117, 2595
Bsh1236I CGCG 2 cut(s) 1102, 2318
BshFI GGCC 7 cut(s) 17, 960, 996, 1352, 2010, 2184, 2646
BshNI GGYRCC 1 cut(s) 2682
BsiHKAI GWGCWC 3 cut(s) 649, 1296, 1776
BsiHKCI CYCGRG 3 cut(s) 508, 1119, 2212
BsiSI CCGG 5 cut(s) 14, 875, 1310, 1665, 2186
BslFI GGGAC 5 cut(s) 185, 1138, 1213, 2480, 2486
BslI CCNNNNNNNGG 6 cut(s) 284, 874, 989, 2237, 2397, 2510
BsmAI GTCTC 6 cut(s) 58, 664, 1250, 1624, 2215, 2471
BsmFI GGGAC 5 cut(s) 185, 1138, 1213, 2480, 2486
BsmI GAATGC 3 cut(s) 316, 523, 1209
BsnI GGCC 7 cut(s) 17, 960, 996, 1352, 2010, 2184, 2646
BsoBI CYCGRG 3 cut(s) 508, 1119, 2212
Bsp1286I GDGCHC 3 cut(s) 649, 1296, 1776
BspANI GGCC 7 cut(s) 17, 960, 996, 1352, 2010, 2184, 2646
BspFNI CGCG 2 cut(s) 1102, 2318
BspLI GGNNCC 5 cut(s) 427, 1188, 2389, 2424, 2684
BspMAI CTGCAG 3 cut(s) 1489, 2331, 2551
BspOI GCTAGC 1 cut(s) 2435
BspPI GGATC 6 cut(s) 728, 1566, 1634, 2222, 2382, 2395
BspT107I GGYRCC 1 cut(s) 2682
BsrDI GCAATG 4 cut(s) 1141, 1653, 2070, 2148
BsrI ACTGG 7 cut(s) 392, 961, 994, 1833, 2011, 2306, 2522
BssECI CCNNGG 6 cut(s) 577, 973, 1983, 2176, 2316, 2557
BssNAI GTATAC 1 cut(s) 2587
BssT1I CCWWGG 4 cut(s) 973, 1983, 2176, 2557
Bst1107I GTATAC 1 cut(s) 2587
Bst2UI CCWGG 6 cut(s) 100, 285, 998, 1268, 1566, 1602
Bst6I CTCTTC 3 cut(s) 113, 1032, 2238
BstAPI GCANNNNNTGC 1 cut(s) 2546
BstC8I GCNNGC 6 cut(s) 523, 1071, 1980, 2103, 2344, 2433
BstDSI CCRYGG 2 cut(s) 577, 2316
BstF5I GGATG 9 cut(s) 9, 216, 489, 527, 710, 815, 1276, 1438, 1726
BstFNI CGCG 2 cut(s) 1102, 2318
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 2 cut(s) 27, 1218
BstMAI GTCTC 6 cut(s) 58, 664, 1250, 1624, 2215, 2471
BstMWI GCNNNNNNNGC 7 cut(s) 957, 1066, 1215, 1667, 2437, 2546, 2609
BstNI CCWGG 6 cut(s) 100, 285, 998, 1268, 1566, 1602
BstNSI RCATGY 3 cut(s) 260, 1982, 2346
BstPAI GACNNNNGTC 1 cut(s) 1262
BstSCI CCNGG 9 cut(s) 12, 98, 283, 996, 1266, 1564, 1600, 1663, 2184
BstSFI CTRYAG 6 cut(s) 1152, 1330, 1485, 1750, 2327, 2547
BstUI CGCG 2 cut(s) 1102, 2318
BstV2I GAAGAC 1 cut(s) 1431
BstX2I RGATCY 2 cut(s) 2310, 2387
BstYI RGATCY 2 cut(s) 2310, 2387
BstZ17I GTATAC 1 cut(s) 2587
BsuI GTATCC 1 cut(s) 811
BsuRI GGCC 7 cut(s) 17, 960, 996, 1352, 2010, 2184, 2646
BtgI CCRYGG 2 cut(s) 577, 2316
BtrI CACGTC 1 cut(s) 1633
BtsCI GGATG 9 cut(s) 9, 216, 489, 527, 710, 815, 1276, 1438, 1726
BtsI GCAGTG 2 cut(s) 333, 1482
BtsIMutI CAGTG 7 cut(s) 333, 346, 851, 1339, 1482, 2136, 2580
Cac8I GCNNGC 6 cut(s) 523, 1071, 1980, 2103, 2344, 2433
CfoI GCGC 2 cut(s) 27, 1218
Cfr13I GGNCC 3 cut(s) 1312, 2183, 2645
Cfr42I CCGCGG 1 cut(s) 2319
CseI GACGC 1 cut(s) 1108
Csp6I GTAC 7 cut(s) 706, 754, 863, 1462, 2062, 2525, 2752
CspCI CAANNNNNGTGG 2 cut(s) 1454, 1489
CviAII CATG 8 cut(s) 82, 257, 451, 1297, 1979, 2343, 2352, 2383
CviQI GTAC 7 cut(s) 706, 754, 863, 1462, 2062, 2525, 2752
DrdI GACNNNNNNGTC 1 cut(s) 1679
DseDI GACNNNNNNGTC 1 cut(s) 1679
EaeI YGGCCR 3 cut(s) 958, 994, 2008
Eam1104I CTCTTC 3 cut(s) 113, 1032, 2238
EarI CTCTTC 3 cut(s) 113, 1032, 2238
EciI GGCGGA 1 cut(s) 240
Eco130I CCWWGG 4 cut(s) 973, 1983, 2176, 2557
Eco32I GATATC 1 cut(s) 1240
Eco47I GGWCC 1 cut(s) 1312
Eco47III AGCGCT 1 cut(s) 26
Eco88I CYCGRG 3 cut(s) 508, 1119, 2212
EcoRII CCWGG 6 cut(s) 98, 283, 996, 1266, 1564, 1600
EcoRV GATATC 1 cut(s) 1240
EcoT14I CCWWGG 4 cut(s) 973, 1983, 2176, 2557
ErhI CCWWGG 4 cut(s) 973, 1983, 2176, 2557
FaeI CATG 8 cut(s) 85, 260, 454, 1300, 1982, 2346, 2355, 2386
FalI AAGNNNNNCTT 2 cut(s) 1451, 1483
FaqI GGGAC 5 cut(s) 185, 1138, 1213, 2480, 2486
FatI CATG 8 cut(s) 81, 256, 450, 1296, 1978, 2342, 2351, 2382
FauI CCCGC 2 cut(s) 739, 2314
FauNDI CATATG 1 cut(s) 2454
FbaI TGATCA 2 cut(s) 594, 1015
FblI GTMKAC 3 cut(s) 1683, 1749, 2586
FokI GGATG 8 cut(s) 203, 476, 514, 697, 822, 1283, 1445, 1713
FspBI CTAG 7 cut(s) 323, 441, 1289, 1406, 1527, 1856, 2432
GlaI GCGC 2 cut(s) 26, 1217
GsaI CCCAGC 1 cut(s) 139
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 7 cut(s) 17, 960, 996, 1352, 2010, 2184, 2646
HapII CCGG 5 cut(s) 14, 875, 1310, 1665, 2186
HgaI GACGC 1 cut(s) 1108
HhaI GCGC 2 cut(s) 27, 1218
Hin1II CATG 8 cut(s) 85, 260, 454, 1300, 1982, 2346, 2355, 2386
Hin6I GCGC 2 cut(s) 25, 1216
HinP1I GCGC 2 cut(s) 25, 1216
HincII GTYRAC 2 cut(s) 1159, 1900
HindII GTYRAC 2 cut(s) 1159, 1900
HindIII AAGCTT 2 cut(s) 623, 1370
HpaII CCGG 5 cut(s) 14, 875, 1310, 1665, 2186
HphI GGTGA 6 cut(s) 319, 566, 775, 1108, 2252, 2306
Hpy166II GTNNAC 7 cut(s) 331, 1159, 1482, 1684, 1750, 1900, 2587
Hpy8I GTNNAC 7 cut(s) 331, 1159, 1482, 1684, 1750, 1900, 2587
Hpy99I CGWCG 2 cut(s) 383, 1772
HpyAV CCTTC 6 cut(s) 467, 471, 920, 1438, 1740, 2413
HpyCH4IV ACGT 2 cut(s) 632, 1632
HpyF10VI GCNNNNNNNGC 7 cut(s) 957, 1066, 1215, 1667, 2437, 2546, 2609
HpySE526I ACGT 2 cut(s) 632, 1632
Hsp92II CATG 8 cut(s) 85, 260, 454, 1300, 1982, 2346, 2355, 2386
HspAI GCGC 2 cut(s) 25, 1216
Ksp22I TGATCA 2 cut(s) 594, 1015
KspI CCGCGG 1 cut(s) 2319
LmnI GCTCC 5 cut(s) 32, 425, 1666, 2087, 2422
LweI GCATC 5 cut(s) 46, 1261, 1423, 2270, 2520
MaeI CTAG 7 cut(s) 323, 441, 1289, 1406, 1527, 1856, 2432
MaeII ACGT 2 cut(s) 632, 1632
MaeIII GTNAC 5 cut(s) 195, 212, 343, 1096, 2575
MfeI CAATTG 2 cut(s) 36, 1392
MflI RGATCY 2 cut(s) 2310, 2387
MhlI GDGCHC 3 cut(s) 649, 1296, 1776
MlsI TGGCCA 3 cut(s) 960, 996, 2010
MluNI TGGCCA 3 cut(s) 960, 996, 2010
MlyI GAGTC 6 cut(s) 902, 1098, 1109, 1421, 2217, 2255
MmeI TCCRAC 2 cut(s) 870, 1125
Mox20I TGGCCA 3 cut(s) 960, 996, 2010
MroXI GAANNNNTTC 1 cut(s) 2696
MscI TGGCCA 3 cut(s) 960, 996, 2010
MslI CAYNNNNRTG 4 cut(s) 726, 761, 1631, 2453
Msp20I TGGCCA 3 cut(s) 960, 996, 2010
MspA1I CMGCKG 5 cut(s) 242, 2067, 2318, 2552, 2609
MspI CCGG 5 cut(s) 14, 875, 1310, 1665, 2186
MspR9I CCNGG 9 cut(s) 14, 100, 285, 998, 1268, 1566, 1602, 1665, 2186
MunI CAATTG 2 cut(s) 36, 1392
Mva1269I GAATGC 3 cut(s) 316, 523, 1209
MvaI CCWGG 6 cut(s) 100, 285, 998, 1268, 1566, 1602
MvnI CGCG 2 cut(s) 1102, 2318
MwoI GCNNNNNNNGC 7 cut(s) 957, 1066, 1215, 1667, 2437, 2546, 2609
NciI CCSGG 3 cut(s) 14, 1665, 2186
NdeI CATATG 1 cut(s) 2454
NheI GCTAGC 1 cut(s) 2431
NlaIII CATG 8 cut(s) 85, 260, 454, 1300, 1982, 2346, 2355, 2386
NlaIV GGNNCC 5 cut(s) 427, 1188, 2389, 2424, 2684
NmuCI GTSAC 2 cut(s) 1096, 2575
NspI RCATGY 3 cut(s) 260, 1982, 2346
OliI CACNNNNGTG 1 cut(s) 726
PaeI GCATGC 2 cut(s) 1982, 2346
PaeR7I CTCGAG 2 cut(s) 508, 1119
PctI GAATGC 3 cut(s) 316, 523, 1209
PdmI GAANNNNTTC 1 cut(s) 2696
PfeI GAWTC 7 cut(s) 32, 336, 586, 611, 640, 1087, 1285
PflFI GACNNNGTC 2 cut(s) 853, 1608
PflMI CCANNNNNTGG 2 cut(s) 284, 2510
PfoI TCCNGGA 1 cut(s) 1266
PleI GAGTC 6 cut(s) 901, 1098, 1109, 1421, 2216, 2254
PpsI GAGTC 6 cut(s) 901, 1098, 1109, 1421, 2216, 2254
PshAI GACNNNNGTC 1 cut(s) 1262
PshBI ATTAAT 1 cut(s) 773
Psp6I CCWGG 6 cut(s) 98, 283, 996, 1266, 1564, 1600
PspFI CCCAGC 1 cut(s) 135
PspGI CCWGG 6 cut(s) 98, 283, 996, 1266, 1564, 1600
PspN4I GGNNCC 5 cut(s) 427, 1188, 2389, 2424, 2684
PspPI GGNCC 3 cut(s) 1312, 2183, 2645
PspXI VCTCGAGB 1 cut(s) 508
PstI CTGCAG 3 cut(s) 1489, 2331, 2551
PsuI RGATCY 2 cut(s) 2310, 2387
PsyI GACNNNGTC 2 cut(s) 853, 1608
PvuII CAGCTG 3 cut(s) 242, 2552, 2609
RsaI GTAC 7 cut(s) 707, 755, 864, 1463, 2063, 2526, 2753
RsaNI GTAC 7 cut(s) 706, 754, 863, 1462, 2062, 2525, 2752
RseI CAYNNNNRTG 4 cut(s) 726, 761, 1631, 2453
SacII CCGCGG 1 cut(s) 2319
Sau96I GGNCC 3 cut(s) 1312, 2183, 2645
ScaI AGTACT 1 cut(s) 864
SchI GAGTC 6 cut(s) 902, 1098, 1109, 1421, 2217, 2255
ScrFI CCNGG 9 cut(s) 14, 100, 285, 998, 1268, 1566, 1602, 1665, 2186
SduI GDGCHC 3 cut(s) 649, 1296, 1776
SfaNI GCATC 5 cut(s) 46, 1261, 1423, 2270, 2520
SfcI CTRYAG 6 cut(s) 1152, 1330, 1485, 1750, 2327, 2547
Sfr274I CTCGAG 2 cut(s) 508, 1119
Sfr303I CCGCGG 1 cut(s) 2319
SgrBI CCGCGG 1 cut(s) 2319
SinI GGWCC 1 cut(s) 1312
SlaI CTCGAG 2 cut(s) 508, 1119
SmiMI CAYNNNNRTG 4 cut(s) 726, 761, 1631, 2453
SmlI CTYRAG 5 cut(s) 494, 508, 1119, 1253, 2741
SmoI CTYRAG 5 cut(s) 494, 508, 1119, 1253, 2741
SpeI ACTAGT 1 cut(s) 1855
SphI GCATGC 2 cut(s) 1982, 2346
SspMI CTAG 7 cut(s) 323, 441, 1289, 1406, 1527, 1856, 2432
StyD4I CCNGG 9 cut(s) 12, 98, 283, 996, 1266, 1564, 1600, 1663, 2184
StyI CCWWGG 4 cut(s) 973, 1983, 2176, 2557
TaiI ACGT 2 cut(s) 635, 1635
TaqI TCGA 9 cut(s) 156, 509, 643, 696, 1120, 1499, 1561, 1612, 1934
TatI WGTACW 3 cut(s) 753, 862, 2751
TauI GCSGC 4 cut(s) 75, 256, 2321, 2606
TfiI GAWTC 7 cut(s) 32, 336, 586, 611, 640, 1087, 1285
TscAI CASTG 7 cut(s) 333, 346, 858, 1339, 1489, 2136, 2580
TseFI GTSAC 2 cut(s) 1096, 2575
Tsp45I GTSAC 2 cut(s) 1096, 2575
TspGWI ACGGA 3 cut(s) 348, 594, 2067
TspRI CASTG 7 cut(s) 333, 346, 858, 1339, 1489, 2136, 2580
Tth111I GACNNNGTC 2 cut(s) 853, 1608
Van91I CCANNNNNTGG 2 cut(s) 284, 2510
VpaK11BI GGWCC 1 cut(s) 1312
VspI ATTAAT 1 cut(s) 773
XapI RAATTY 5 cut(s) 359, 766, 1448, 1721, 1891
XbaI TCTAGA 2 cut(s) 1288, 1405
XceI RCATGY 3 cut(s) 260, 1982, 2346
XcmI CCANNNNNNNNNTGG 1 cut(s) 1741
XhoI CTCGAG 2 cut(s) 508, 1119
XmiI GTMKAC 3 cut(s) 1683, 1749, 2586
XmnI GAANNNNTTC 1 cut(s) 2696
XspI CTAG 7 cut(s) 323, 441, 1289, 1406, 1527, 1856, 2432
ZrmI AGTACT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.