Rh7AG005400

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0007625
Physical Location & Seq
Reverse (-)
377208 .. 381027
3820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG005400.1

Sequence Viewer

Length: 2742 bp
ATGGCTTCCATCCCGGCCTTGAAGCGCTCCGATTCAATTGCAGACACTATGCCAGATGCTCTGAGACAGAGCCGCTATCACATGAAGAAATGTTTTGCCAGGTTTGTCGGGAATGGGAAGAGGTTAATGAAGCCCCAGCATATAATGGAGGAAATCGAAAAATCAATAGGGGACGGGAATGAAAAAAGCAAGGTGTTACGGGGATTACTTGGTTACATCCTCAGTTCCACTCAGGAAGCAGCTGTTGTTCCGCCGCATGTTGCATTTTCTGTTAGACCAAATCCTGGTTTCTCAGAGTTTGTTAAGGTGAATGCTGATGATCTAGCAGTGGATGGAATCAGTGTTACCGAATACTTGAAATTCAAAGAAATGATCTACGACGAGAACTGGGCAAATGATGAAAATGCTTTGGAAATTGATTTTGGAGCCATTGACTTCTCTAGTCCTCGCATGACCCTTCCTTCTTCTATTGGGAATGGACTTAACTTCATCCTCAAGTTAATCTCCTCGAGGCTGAATGCCAGCTCATCCGATTATGCCAAACCCTTGCTTGACTACTTGTTAGCACTCAATCACCACGGACAGAATCTTATGATCAATGAAACTATGGATTCGGTGGAAAAGCTTGAAACGTCATTGATTCGAGCACAGTCGTTTGTCTCAGCACTCCCAAAAAACACACCATTTCTTAATTTCGAGCAGAGGTTAAAGGATATGGGTTTTGAGAAAGGATGGGGGGATACAGCAGAAAGAGTTGTTGAGAGAATGAGGACACTGTCAGAAGTACTCCAAGCACCGGATTGCGTGAAGTTGGAGTCATTCTTTAGCAGCCTTCCCAATACATTCAACATTGTCATCTTCTCTCCTCACGGCTACTTTGGCCAGTCAGATGTCCTTGGATTGCCTGATACTGGTGGCCAGGTGGTTTATATACTTGATCAAGTGAGAGCCTTAGAGGAAGAGTTGCTGATGAGAATAAAGCAGCAAGGGCTTGCTGTGAAGCCTCAGATTCTTGTGGTGACGCGACTCATACCAGACTCTCGAGGGACTAAGTGCAATGTGGAGTTGGAGGCTATAGTCAACACTAAGCACTCCCACATTCTCAGGGTTCCTTTCAGGACAGACAAAGGCATTCTGCGCCATTGGGTGTCCCGTTTTGATATCTACCCTTATCTTGAGACTTTTGTCCAGGATGCAACTGCAAAGATTCTAGAGCACATGGACTGCAAACCGGACCTCATAATTGGGAACTACAGTGATGGAAACTTGGTGGCCTCTTTGATGGCTGCCAAGCTTGGAATAACACAGGGAACAATTGCCCACGCTCTAGAAAAGACTAAGTATGAAGACTCGGATGCCAAATGGAAGGAATTTGATACAAAGTACCACTTTTCTTGTCAGTTCACTGCAGACTTAATCTCGATGAACTCAACTGATTTTGTCATAACTAGCACATTTCAAGAAATAGCAGGAGGTAAGGATCGACCTGGACAGTATGAAAGCCACACCGCATTTACAATGCCAGGACTGTGTCGAGTAGTCTCAGGCATTGACGTGTTTGATCCGAAGTTCAACATTGCTGCTCCCGGTGCTGACCAATCTGTCTACTTCCCCAACTCCGAAAAACAGAAGCGTTTTACCAAATTTCATCCTTCCATTGAACAAATGGTCTACAGTAAGGAGGAAAACGACGAGCACATAGGATTTTTGGCAGACAAAAAGAAACCAATCATCTTTTCAATGGCAAGACTGGATAAAGTGAAGAACCTCTCAGGACTAGTTGAGTGGTTCGGAAAGAACAAGAGGCTGAGGAATTTGGTCAACCTTGTTATAGTAGGGGGGTTCTTTGACCCATCCAAGTCCAAAGACAGAGAAGAAATTGCTGAAATTATCAAACTGCATGCCTTGGTTAAAGAATATCAACTTAATGGCCAGTTCAGATGGATAGCAGCTCAAACTGATAGGTATCGCAACGGAGAGTTGTACCGCTGCATTGCTGATACAAAGGGAGCTTTTGTGCAGCCTGCATTGTATGAAGCCTTTGGTCTTACAGTGATTGAGGCAATGAACTGTGGATTGCCCTCTTTTGCTACAAACCAAGGAGGCCCGGCTGAAATTATTGTTGATGGAGTCTCGGGCTTCCATATTGATCCCAACAATGGTGAAGAGTCCAGCAACAAGATTGCTGATTTCTTTGAGAGATGCAGGGCAGATGGTGAATACTGGAAGAAGATCTCCGCGGCGGGTCTGCAGCGTATATACGCATGCTATACATGGAAAATATACGCAAACAAAATGTTGAACATGGGATCCACTTATGGGTTTTGGAGGCAGTTGAGGAAGGAGCCGAAGCTAGCAAAGCAAAGCTACATTCATATGTTTTACAGTCTCCTATTTAGGACTTTGGCAAGGACTGTCCCTGTCCCAAGTGATGGATATGAACCAGTACCGATGCCAAAGGCAATAGCTGCAGCTGACCAAGGCAAACCAAAGTCAGTGACAACTGTATCCAAACCCCCACAAGCCGCAGCTGCACCAACAATGCCAACAGTTTCAGTTGCTCAGATCACACCAACACCAAGGGATGAAGGAGGTTTAGAGCAGATAAATAAGCAACCTATTGGCCCTCCTGATGTTGCAAGTCCAAACCGCCCAACACGGTGCCTTTGGAATTGCTTCTTCGTCATCATTGGTTTCCTGTGCCTTGTTTATTACAAGCTCAAGAATATGTACTACAAATGA

Protein Analysis

913

Amino Acids

102.57

Weight (kDa)

8.03

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 9 - 123 2.1e-45 Sucrose synthase N-terminal
SUS_EPBD PF24862 159 - 247 6.2e-27 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 270 - 558 1e-167 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 569 - 733 2e-29 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 576 - 731 1.1e-13 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1601
AbsI CCTCGAGG 1 cut(s) 508
AccB1I GGYRCC 1 cut(s) 2661
AccB7I CCANNNNNTGG 2 cut(s) 284, 2432
AccI GTMKAC 2 cut(s) 1605, 1671
AccII CGCG 2 cut(s) 1024, 2240
AclWI GGATC 5 cut(s) 1488, 1556, 2144, 2304, 2317
AcoI YGGCCR 3 cut(s) 880, 916, 1930
AcsI RAATTY 4 cut(s) 359, 1370, 1643, 1813
AfaI GTAC 5 cut(s) 786, 1385, 1985, 2448, 2732
AfeI AGCGCT 1 cut(s) 26
AfiI CCNNNNNNNGG 6 cut(s) 284, 796, 911, 2159, 2319, 2432
AflIII ACRYGT 1 cut(s) 1554
AhlI ACTAGT 1 cut(s) 1777
AjiI CACGTC 1 cut(s) 1555
AjnI CCWGG 6 cut(s) 98, 283, 918, 1188, 1486, 1522
AjuI GAANNNNNNNTTGG 4 cut(s) 405, 437, 1720, 1752
AloI GAACNNNNNNTCC 2 cut(s) 2294, 2326
Alw21I GWGCWC 3 cut(s) 649, 1218, 1698
Alw26I GTCTC 6 cut(s) 58, 664, 1172, 1546, 2137, 2393
AlwI GGATC 5 cut(s) 1488, 1556, 2144, 2304, 2317
Ama87I CYCGRG 3 cut(s) 508, 1041, 2134
Aor51HI AGCGCT 1 cut(s) 26
AoxI GGCC 7 cut(s) 15, 880, 916, 1272, 1930, 2104, 2623
ApoI RAATTY 4 cut(s) 359, 1370, 1643, 1813
Asp700I GAANNNNTTC 1 cut(s) 2675
AspLEI GCGC 2 cut(s) 27, 1140
AspS9I GGNCC 3 cut(s) 1234, 2105, 2624
AsuC2I CCSGG 3 cut(s) 14, 1587, 2108
AsuHPI GGTGA 5 cut(s) 319, 566, 1030, 2174, 2228
AsuNHI GCTAGC 1 cut(s) 2353
AvaI CYCGRG 3 cut(s) 508, 1041, 2134
AvaII GGWCC 1 cut(s) 1234
BaeI ACNNNNGTAYC 2 cut(s) 2490, 2523
BalI TGGCCA 3 cut(s) 882, 918, 1932
BamHI GGATCC 1 cut(s) 2309
BanI GGYRCC 1 cut(s) 2661
BbsI GAAGAC 1 cut(s) 1353
Bbv12I GWGCWC 3 cut(s) 649, 1218, 1698
BbvCI CCTCAGC 1 cut(s) 1808
BceAI ACGGC 1 cut(s) 886
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 6 cut(s) 100, 285, 920, 1190, 1488, 1524
BciVI GTATCC 2 cut(s) 733, 2518
BclI TGATCA 2 cut(s) 594, 937
BcnI CCSGG 3 cut(s) 14, 1587, 2108
BcoDI GTCTC 6 cut(s) 58, 664, 1172, 1546, 2137, 2393
BcuI ACTAGT 1 cut(s) 1777
BfaI CTAG 7 cut(s) 323, 441, 1211, 1328, 1449, 1778, 2354
BfmI CTRYAG 6 cut(s) 1074, 1252, 1407, 1672, 2249, 2469
BfoI RGCGCY 1 cut(s) 28
BfuI GTATCC 2 cut(s) 733, 2518
BglII AGATCT 1 cut(s) 2232
BmcAI AGTACT 1 cut(s) 786
Bme1390I CCNGG 9 cut(s) 14, 100, 285, 920, 1190, 1488, 1524, 1587, 2108
Bme18I GGWCC 1 cut(s) 1234
BmeT110I CYCGRG 3 cut(s) 508, 1041, 2134
BmgBI CACGTC 1 cut(s) 1555
BmgT120I GGNCC 3 cut(s) 1234, 2105, 2624
BmiI GGNNCC 5 cut(s) 427, 1110, 2311, 2346, 2663
BmrFI CCNGG 9 cut(s) 14, 100, 285, 920, 1190, 1488, 1524, 1587, 2108
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 5 cut(s) 46, 1183, 1345, 2192, 2442
BmtI GCTAGC 1 cut(s) 2357
BmuI ACTGGG 1 cut(s) 397
BoxI GACNNNNGTC 1 cut(s) 1184
BpiI GAAGAC 1 cut(s) 1353
Bpu10I CCTNAGC 1 cut(s) 1808
BpuEI CTTGAG 3 cut(s) 479, 1196, 2705
BpuMI CCSGG 3 cut(s) 14, 1587, 2108
BsaBI GATNNNNATC 1 cut(s) 1965
BsaJI CCNNGG 7 cut(s) 577, 895, 1905, 2098, 2238, 2479, 2579
BsaWI WCCGGW 2 cut(s) 796, 1231
BsaXI ACNNNNNCTCC 2 cut(s) 2001, 2031
Bsc4I CCNNNNNNNGG 6 cut(s) 284, 796, 911, 2159, 2319, 2432
Bse1I ACTGG 7 cut(s) 392, 883, 916, 1755, 1933, 2228, 2444
Bse3DI GCAATG 4 cut(s) 1063, 1575, 1992, 2070
Bse8I GATNNNNATC 1 cut(s) 1965
BseBI CCWGG 6 cut(s) 100, 285, 920, 1190, 1488, 1524
BseDI CCNNGG 7 cut(s) 577, 895, 1905, 2098, 2238, 2479, 2579
BseJI GATNNNNATC 1 cut(s) 1965
BseLI CCNNNNNNNGG 6 cut(s) 284, 796, 911, 2159, 2319, 2432
BseMI GCAATG 4 cut(s) 1063, 1575, 1992, 2070
BseNI ACTGG 7 cut(s) 392, 883, 916, 1755, 1933, 2228, 2444
BseRI GAGGAG 2 cut(s) 496, 855
BseYI CCCAGC 1 cut(s) 135
BsgI GTGCAG 2 cut(s) 2039, 2517
Bsh1236I CGCG 2 cut(s) 1024, 2240
BshFI GGCC 7 cut(s) 17, 882, 918, 1274, 1932, 2106, 2625
BshNI GGYRCC 1 cut(s) 2661
BsiHKAI GWGCWC 3 cut(s) 649, 1218, 1698
BsiHKCI CYCGRG 3 cut(s) 508, 1041, 2134
BsiSI CCGG 5 cut(s) 14, 797, 1232, 1587, 2108
BslFI GGGAC 5 cut(s) 185, 1060, 1135, 2402, 2408
BslI CCNNNNNNNGG 6 cut(s) 284, 796, 911, 2159, 2319, 2432
BsmAI GTCTC 6 cut(s) 58, 664, 1172, 1546, 2137, 2393
BsmFI GGGAC 5 cut(s) 185, 1060, 1135, 2402, 2408
BsmI GAATGC 3 cut(s) 316, 523, 1131
BsnI GGCC 7 cut(s) 17, 882, 918, 1274, 1932, 2106, 2625
BsoBI CYCGRG 3 cut(s) 508, 1041, 2134
Bsp1286I GDGCHC 3 cut(s) 649, 1218, 1698
BspANI GGCC 7 cut(s) 17, 882, 918, 1274, 1932, 2106, 2625
BspFNI CGCG 2 cut(s) 1024, 2240
BspLI GGNNCC 5 cut(s) 427, 1110, 2311, 2346, 2663
BspMAI CTGCAG 3 cut(s) 1411, 2253, 2473
BspOI GCTAGC 1 cut(s) 2357
BspPI GGATC 5 cut(s) 1488, 1556, 2144, 2304, 2317
BspT107I GGYRCC 1 cut(s) 2661
BsrDI GCAATG 4 cut(s) 1063, 1575, 1992, 2070
BsrI ACTGG 7 cut(s) 392, 883, 916, 1755, 1933, 2228, 2444
BssECI CCNNGG 7 cut(s) 577, 895, 1905, 2098, 2238, 2479, 2579
BssT1I CCWWGG 5 cut(s) 895, 1905, 2098, 2479, 2579
Bst2UI CCWGG 6 cut(s) 100, 285, 920, 1190, 1488, 1524
Bst6I CTCTTC 3 cut(s) 113, 954, 2160
BstAPI GCANNNNNTGC 1 cut(s) 2468
BstC8I GCNNGC 6 cut(s) 523, 993, 1902, 2025, 2266, 2355
BstDSI CCRYGG 2 cut(s) 577, 2238
BstFNI CGCG 2 cut(s) 1024, 2240
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 2 cut(s) 27, 1140
BstMAI GTCTC 6 cut(s) 58, 664, 1172, 1546, 2137, 2393
BstMWI GCNNNNNNNGC 7 cut(s) 879, 988, 1137, 1589, 2359, 2468, 2531
BstNI CCWGG 6 cut(s) 100, 285, 920, 1190, 1488, 1524
BstNSI RCATGY 3 cut(s) 260, 1904, 2268
BstPAI GACNNNNGTC 1 cut(s) 1184
BstSCI CCNGG 9 cut(s) 12, 98, 283, 918, 1188, 1486, 1522, 1585, 2106
BstSFI CTRYAG 6 cut(s) 1074, 1252, 1407, 1672, 2249, 2469
BstUI CGCG 2 cut(s) 1024, 2240
BstV2I GAAGAC 1 cut(s) 1353
BstX2I RGATCY 2 cut(s) 2232, 2309
BstYI RGATCY 2 cut(s) 2232, 2309
BsuI GTATCC 2 cut(s) 733, 2518
BsuRI GGCC 7 cut(s) 17, 882, 918, 1274, 1932, 2106, 2625
BtgI CCRYGG 2 cut(s) 577, 2238
BtrI CACGTC 1 cut(s) 1555
BtsI GCAGTG 2 cut(s) 333, 1404
BtsIMutI CAGTG 7 cut(s) 333, 346, 773, 1261, 1404, 2058, 2502
Cac8I GCNNGC 6 cut(s) 523, 993, 1902, 2025, 2266, 2355
CfoI GCGC 2 cut(s) 27, 1140
Cfr13I GGNCC 3 cut(s) 1234, 2105, 2624
Cfr42I CCGCGG 1 cut(s) 2241
CseI GACGC 1 cut(s) 1030
Csp6I GTAC 5 cut(s) 785, 1384, 1984, 2447, 2731
CspCI CAANNNNNGTGG 2 cut(s) 1376, 1411
CviAII CATG 8 cut(s) 82, 257, 451, 1219, 1901, 2265, 2274, 2305
CviQI GTAC 5 cut(s) 785, 1384, 1984, 2447, 2731
DrdI GACNNNNNNGTC 1 cut(s) 1601
DseDI GACNNNNNNGTC 1 cut(s) 1601
EaeI YGGCCR 3 cut(s) 880, 916, 1930
Eam1104I CTCTTC 3 cut(s) 113, 954, 2160
EarI CTCTTC 3 cut(s) 113, 954, 2160
EciI GGCGGA 1 cut(s) 240
Eco130I CCWWGG 5 cut(s) 895, 1905, 2098, 2479, 2579
Eco32I GATATC 1 cut(s) 1162
Eco47I GGWCC 1 cut(s) 1234
Eco47III AGCGCT 1 cut(s) 26
Eco88I CYCGRG 3 cut(s) 508, 1041, 2134
EcoRII CCWGG 6 cut(s) 98, 283, 918, 1188, 1486, 1522
EcoRV GATATC 1 cut(s) 1162
EcoT14I CCWWGG 5 cut(s) 895, 1905, 2098, 2479, 2579
ErhI CCWWGG 5 cut(s) 895, 1905, 2098, 2479, 2579
FaeI CATG 8 cut(s) 85, 260, 454, 1222, 1904, 2268, 2277, 2308
FalI AAGNNNNNCTT 2 cut(s) 1373, 1405
FaqI GGGAC 5 cut(s) 185, 1060, 1135, 2402, 2408
FatI CATG 8 cut(s) 81, 256, 450, 1218, 1900, 2264, 2273, 2304
FauI CCCGC 1 cut(s) 2236
FauNDI CATATG 1 cut(s) 2376
FbaI TGATCA 2 cut(s) 594, 937
FblI GTMKAC 2 cut(s) 1605, 1671
FspBI CTAG 7 cut(s) 323, 441, 1211, 1328, 1449, 1778, 2354
GlaI GCGC 2 cut(s) 26, 1139
GsaI CCCAGC 1 cut(s) 139
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 7 cut(s) 17, 882, 918, 1274, 1932, 2106, 2625
HapII CCGG 5 cut(s) 14, 797, 1232, 1587, 2108
HgaI GACGC 1 cut(s) 1030
HhaI GCGC 2 cut(s) 27, 1140
Hin1II CATG 8 cut(s) 85, 260, 454, 1222, 1904, 2268, 2277, 2308
Hin6I GCGC 2 cut(s) 25, 1138
HinP1I GCGC 2 cut(s) 25, 1138
HincII GTYRAC 2 cut(s) 1081, 1822
HindII GTYRAC 2 cut(s) 1081, 1822
HindIII AAGCTT 2 cut(s) 623, 1292
HpaII CCGG 5 cut(s) 14, 797, 1232, 1587, 2108
HphI GGTGA 5 cut(s) 319, 566, 1030, 2174, 2228
Hpy166II GTNNAC 5 cut(s) 1081, 1404, 1606, 1672, 1822
Hpy8I GTNNAC 5 cut(s) 1081, 1404, 1606, 1672, 1822
Hpy99I CGWCG 2 cut(s) 383, 1694
HpyAV CCTTC 7 cut(s) 467, 471, 842, 1360, 1662, 2335, 2582
HpyCH4IV ACGT 2 cut(s) 632, 1554
HpyF10VI GCNNNNNNNGC 7 cut(s) 879, 988, 1137, 1589, 2359, 2468, 2531
HpySE526I ACGT 2 cut(s) 632, 1554
Hsp92II CATG 8 cut(s) 85, 260, 454, 1222, 1904, 2268, 2277, 2308
HspAI GCGC 2 cut(s) 25, 1138
Ksp22I TGATCA 2 cut(s) 594, 937
KspI CCGCGG 1 cut(s) 2241
LmnI GCTCC 5 cut(s) 32, 425, 1588, 2009, 2344
LweI GCATC 5 cut(s) 46, 1183, 1345, 2192, 2442
MaeI CTAG 7 cut(s) 323, 441, 1211, 1328, 1449, 1778, 2354
MaeII ACGT 2 cut(s) 632, 1554
MaeIII GTNAC 5 cut(s) 195, 212, 343, 1018, 2497
MfeI CAATTG 2 cut(s) 36, 1314
MflI RGATCY 2 cut(s) 2232, 2309
MhlI GDGCHC 3 cut(s) 649, 1218, 1698
MlsI TGGCCA 3 cut(s) 882, 918, 1932
MluNI TGGCCA 3 cut(s) 882, 918, 1932
MlyI GAGTC 6 cut(s) 824, 1020, 1031, 1343, 2139, 2177
MmeI TCCRAC 2 cut(s) 792, 1047
Mox20I TGGCCA 3 cut(s) 882, 918, 1932
MroXI GAANNNNTTC 1 cut(s) 2675
MscI TGGCCA 3 cut(s) 882, 918, 1932
MseI TTAA 9 cut(s) 125, 303, 483, 500, 690, 707, 1415, 1911, 1926
MslI CAYNNNNRTG 2 cut(s) 1553, 2375
Msp20I TGGCCA 3 cut(s) 882, 918, 1932
MspA1I CMGCKG 5 cut(s) 242, 1989, 2240, 2474, 2531
MspI CCGG 5 cut(s) 14, 797, 1232, 1587, 2108
MspR9I CCNGG 9 cut(s) 14, 100, 285, 920, 1190, 1488, 1524, 1587, 2108
MunI CAATTG 2 cut(s) 36, 1314
Mva1269I GAATGC 3 cut(s) 316, 523, 1131
MvaI CCWGG 6 cut(s) 100, 285, 920, 1190, 1488, 1524
MvnI CGCG 2 cut(s) 1024, 2240
MwoI GCNNNNNNNGC 7 cut(s) 879, 988, 1137, 1589, 2359, 2468, 2531
NciI CCSGG 3 cut(s) 14, 1587, 2108
NdeI CATATG 1 cut(s) 2376
NheI GCTAGC 1 cut(s) 2353
NlaIII CATG 8 cut(s) 85, 260, 454, 1222, 1904, 2268, 2277, 2308
NlaIV GGNNCC 5 cut(s) 427, 1110, 2311, 2346, 2663
NmuCI GTSAC 2 cut(s) 1018, 2497
NspI RCATGY 3 cut(s) 260, 1904, 2268
PaeI GCATGC 2 cut(s) 1904, 2268
PaeR7I CTCGAG 2 cut(s) 508, 1041
PctI GAATGC 3 cut(s) 316, 523, 1131
PdmI GAANNNNTTC 1 cut(s) 2675
PfeI GAWTC 7 cut(s) 32, 336, 586, 611, 640, 1009, 1207
PflFI GACNNNGTC 2 cut(s) 775, 1530
PflMI CCANNNNNTGG 2 cut(s) 284, 2432
PfoI TCCNGGA 1 cut(s) 1188
PleI GAGTC 6 cut(s) 823, 1020, 1031, 1343, 2138, 2176
PpsI GAGTC 6 cut(s) 823, 1020, 1031, 1343, 2138, 2176
PshAI GACNNNNGTC 1 cut(s) 1184
Psp6I CCWGG 6 cut(s) 98, 283, 918, 1188, 1486, 1522
PspFI CCCAGC 1 cut(s) 135
PspGI CCWGG 6 cut(s) 98, 283, 918, 1188, 1486, 1522
PspN4I GGNNCC 5 cut(s) 427, 1110, 2311, 2346, 2663
PspPI GGNCC 3 cut(s) 1234, 2105, 2624
PspXI VCTCGAGB 1 cut(s) 508
PstI CTGCAG 3 cut(s) 1411, 2253, 2473
PsuI RGATCY 2 cut(s) 2232, 2309
PsyI GACNNNGTC 2 cut(s) 775, 1530
PvuII CAGCTG 3 cut(s) 242, 2474, 2531
RsaI GTAC 5 cut(s) 786, 1385, 1985, 2448, 2732
RsaNI GTAC 5 cut(s) 785, 1384, 1984, 2447, 2731
RseI CAYNNNNRTG 2 cut(s) 1553, 2375
SacII CCGCGG 1 cut(s) 2241
SaqAI TTAA 9 cut(s) 125, 303, 483, 500, 690, 707, 1415, 1911, 1926
Sau96I GGNCC 3 cut(s) 1234, 2105, 2624
ScaI AGTACT 1 cut(s) 786
SchI GAGTC 6 cut(s) 824, 1020, 1031, 1343, 2139, 2177
ScrFI CCNGG 9 cut(s) 14, 100, 285, 920, 1190, 1488, 1524, 1587, 2108
SduI GDGCHC 3 cut(s) 649, 1218, 1698
SfaNI GCATC 5 cut(s) 46, 1183, 1345, 2192, 2442
SfcI CTRYAG 6 cut(s) 1074, 1252, 1407, 1672, 2249, 2469
Sfr274I CTCGAG 2 cut(s) 508, 1041
Sfr303I CCGCGG 1 cut(s) 2241
SgrBI CCGCGG 1 cut(s) 2241
SinI GGWCC 1 cut(s) 1234
SlaI CTCGAG 2 cut(s) 508, 1041
SmiMI CAYNNNNRTG 2 cut(s) 1553, 2375
SmlI CTYRAG 5 cut(s) 494, 508, 1041, 1175, 2720
SmoI CTYRAG 5 cut(s) 494, 508, 1041, 1175, 2720
SpeI ACTAGT 1 cut(s) 1777
SphI GCATGC 2 cut(s) 1904, 2268
SspMI CTAG 7 cut(s) 323, 441, 1211, 1328, 1449, 1778, 2354
StyD4I CCNGG 9 cut(s) 12, 98, 283, 918, 1188, 1486, 1522, 1585, 2106
StyI CCWWGG 5 cut(s) 895, 1905, 2098, 2479, 2579
TaiI ACGT 2 cut(s) 635, 1557
TaqI TCGA 8 cut(s) 156, 509, 643, 696, 1042, 1421, 1483, 1534
TatI WGTACW 2 cut(s) 784, 2730
TauI GCSGC 4 cut(s) 75, 256, 2243, 2528
TfiI GAWTC 7 cut(s) 32, 336, 586, 611, 640, 1009, 1207
Tru1I TTAA 9 cut(s) 125, 303, 483, 500, 690, 707, 1415, 1911, 1926
Tru9I TTAA 9 cut(s) 125, 303, 483, 500, 690, 707, 1415, 1911, 1926
TscAI CASTG 7 cut(s) 333, 346, 780, 1261, 1411, 2058, 2502
TseFI GTSAC 2 cut(s) 1018, 2497
Tsp45I GTSAC 2 cut(s) 1018, 2497
TspGWI ACGGA 2 cut(s) 594, 1989
TspRI CASTG 7 cut(s) 333, 346, 780, 1261, 1411, 2058, 2502
Tth111I GACNNNGTC 2 cut(s) 775, 1530
Van91I CCANNNNNTGG 2 cut(s) 284, 2432
VpaK11BI GGWCC 1 cut(s) 1234
XapI RAATTY 4 cut(s) 359, 1370, 1643, 1813
XbaI TCTAGA 2 cut(s) 1210, 1327
XceI RCATGY 3 cut(s) 260, 1904, 2268
XcmI CCANNNNNNNNNTGG 1 cut(s) 1663
XhoI CTCGAG 2 cut(s) 508, 1041
XmiI GTMKAC 2 cut(s) 1605, 1671
XmnI GAANNNNTTC 1 cut(s) 2675
XspI CTAG 7 cut(s) 323, 441, 1211, 1328, 1449, 1778, 2354
ZrmI AGTACT 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.