MD06G1237200.v1.1

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007625
Physical Location & Seq
Forward (+)
36846259 .. 36850391
4133 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1237200.v1.1.491

Sequence Viewer

Length: 2700 bp
ATGGCTTCCACCTCCTCAGCTTTGAAGCGATCTGATACGATTGCAGAAACCATGCCAGATGCACTCAGGGAGAGCCGCTTTCATATGAAGAAATGTTTTGCCAGTTTTGTTGGAACGGGGAAAAGGCTAATAAAGCCCCAGCATATAATGGAGGAGCTGGAAAAATCGATAGAAGACAGGCATGAAAGAAGCAAGGTCTTGGAGGGTTTACTTGGTTACATCCTCAGTCGTACTCAGGAGGCAGCTGTTGTTCCACCGTATGTCGCTTTTGCTGTGAGACCGAATCCCGGTTTCTCCGAATTTGTTAAGGTGAATGCAGATGACCTAGCAGTGGATGGCATTTCTGCTACCCAATACTTGAAGTTCAAAGAAATGATCTTTGACGAAAGCTGGGCGAACGATGAAAATGCTTTGGAAATAGATTTTGGAGCCTTCGATTTCTCTACTCCTCGAATGACTCTTCCTTCTTCGATCGGAAATGGACTCAACTTCGTCTTAAAGTTAATCTCATCAAGGCTCAGTACTCATGCCAGCTGCTCCGATTATGCTAAACCCCTCCTTGACTACTTATTACCACTTAATTATCACGGAGAGAATCTTATGATCAATGAAAGTCTGGATACCGTTGAAAAACTCCAAACCGCATTGATCCGAGCAGAAGTACTCGTCTCCACACTTCCAAAAACCACACCATTTCCGAATTTTGAGCAAAGGTTTAAAGTGTTGGGTTTTGAGAAGGGATGGGGAGATACAGCAGAAAGAGTTGGGGAGACAATGAGGTTGCTGTCAGAAGTACTCCAAGCGCCAGACTCGGTGAAGTTGGAGTCGTTGTTTAGCAGACTTCCTAACACATTCAACATCGTTATCTTCTCTCCTCACGGCTACTTTGGCCAGTCGAATGTCCTTGGATTGCCCGACACGGGTGGCCAGGTGGTTTATATTCTTGATCAAGTGAGAGCTTTGGAGGAAGAGTTGCTTCTCAGAATAAAACAACAAGGCCTTGCTGTGAAACCTCAGATTCTTGTGGTGACACGACTTATACCAGACGCTCGAGGAACAAAATGCAACCAGGAGTTGGAAGCTATCATCGACACTAAGCACTCCCACATCCTTAGGGTTCCATTCAGGACAGAAAAAGGCGTTCTCCGCCAATGGGTTTCTCGTTTCGATATCTACCCTTATCTCGAGACATTTGCACTGGATGCAACTGCAAAGATCTTACGACACATGGAATGCAAACCGGACCTCATAATTGGGAACTACAGTGATGGAAACCTGGTGGCCTCTCTGATTGCTTCTAAACTTGGAATCACTCAGGGAACGATTGCGCATGCGCTAGAAAAGACCAAGTACGAAGATTCTGATGCCAAATGGAAGGAATTTGATCCAAAATACCACTTCTCTTGTCAGTTCACAGCTGACATAATCTCTATGAATTGTGCTGATTTTGTCATAACAAGCACATTCCAAGAAATTGCCGGAGGCAAGGACCGACCTGGACAGTACGAAAGCCATACAGCATTTACAATGCCAGGACTTTATCGAGTCGTGTCAGGCATTGATGTGTTTGATCCAAAGTTCAACATTGCTGCCCCTGGTGCTGACCAATCCGTCTACTTTCCCTACTCCGAGAAACAAAAACGATTTACCAAGTTTCAACCTGCCATCGAAGAACTACTCTACAAGAAAGAGGAAAACGATGAGCACATAGGATTTTTGGCAGACCAGAAGAAACCGATCATATTCTCAATGGCAAGGCTGGACACAGTGAAGAACCTCACAGGACTCGTTGAGTGGTTTGGAAAGAACAAGAGGCTGAGGAATTTGGTCAATCTTGTAATCGTTGGGGGATTCTTCGATCCATCTAAATCCAAAGACAGAGAAGAAATTGCTGAAATCAAGAAACTGCATGCCTTGGTGCAAGAATATCAACTTAGCGGTCAGTTCAGATGGATAGCGGCTCAGACTGACAGATACCGAAATGGGGAGCTGTACCGGTGCATTGCAGACACAAAGGGATCTTTCGTGCAGCCTGCGCTGTACGAAGCGTTCGGTCTCACAGTCATTGAGGCCATGAACTGTGGGTTGCCTACATTTGCAACCAACCAAGGAGGCCCTGCAGAAATCATTGTGGATGGCGTCTCAGGTTTCCATATTGATCCCAACAATGGTGATGAATCCAGCAACAAGATTGCTGACTTCTTTGAGAAATGCAAGACGGATGGCGAATACTGGAAGAAGATGTCAGCTGCCGGTCTGCAGCGTATAAACGAATGCTATACATGGAAGATATATGCAAACAAAGTGTTGAACATGGGATCAACTTATGGATTTTGGAGGCAGTTGAGAGACGCGCAAAAGTTAGCCAAGGAAACCTACATTCATATGTTTTACAATCTCCTCTTTAGGAAATTGGCAAAGAATGTGGCTGTCCCAAGTGATGGATACGAACAACCGGCGCCAAAGGCCGTAACTGCATCTGTCGACCAACGCGCGCCGGCAGCAGTATCCAAACCTCCGCAGCCAGATGCAGCACCAACCTTAGCAATCCCTCAGCTGACACCAAGGGAGAGGGATGAAGGTGGGGAGCTTGGCCAACCTCGAAGCCGCAGCAGAGCACGATGCCCATGGAATTGCTGCTGCGTCATCCTTGGTTTTCTCATCATTCTTTATTACAAGATTAGGAATATGTACAATTAA

Protein Analysis

900

Amino Acids

101.28

Weight (kDa)

6.79

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUS_N PF24861 10 - 124 4.7e-44 Sucrose synthase N-terminal
SUS_EPBD PF24862 160 - 250 3.3e-25 Sucrose synthase EPBD domain
GT-B_Sucrose_synth PF00862 273 - 561 2e-169 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 573 - 738 6.1e-30 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 579 - 734 1.5e-13 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1610
Acc16I TGCGCA 1 cut(s) 1329
Acc36I ACCTGC 1 cut(s) 1669
AccB1I GGYRCC 1 cut(s) 2458
AccB7I CCANNNNNTGG 2 cut(s) 1075, 2441
AccI GTMKAC 2 cut(s) 1614, 2484
AccII CGCG 3 cut(s) 2354, 2493, 2495
AciI CCGC 7 cut(s) 76, 642, 1147, 1938, 1958, 2519, 2608
AclWI GGATC 7 cut(s) 643, 1379, 1565, 1853, 2026, 2153, 2326
AcoI YGGCCR 3 cut(s) 889, 925, 2593
AcsI RAATTY 4 cut(s) 299, 700, 1379, 1822
AcyI GRCGYC 2 cut(s) 2139, 2459
AfaI GTAC 9 cut(s) 232, 523, 663, 795, 1352, 1505, 1994, 2042, 2693
AfiI CCNNNNNNNGG 8 cut(s) 287, 331, 920, 1075, 1153, 1984, 2168, 2441
AgeI ACCGGT 1 cut(s) 1995
AgsI TTSAA 8 cut(s) 25, 361, 367, 629, 856, 1582, 1658, 2311
AjnI CCWGG 6 cut(s) 927, 1068, 1275, 1495, 1531, 1594
AjuI GAANNNNNNNTTGG 2 cut(s) 408, 440
Alw21I GWGCWC 2 cut(s) 1707, 2620
Alw26I GTCTC 7 cut(s) 271, 673, 764, 1181, 2060, 2146, 2343
AlwI GGATC 7 cut(s) 643, 1379, 1565, 1853, 2026, 2153, 2326
Ama87I CYCGRG 2 cut(s) 1050, 1184
AoxI GGCC 8 cut(s) 889, 925, 997, 1281, 2070, 2113, 2466, 2593
ApoI RAATTY 4 cut(s) 299, 700, 1379, 1822
AsiGI ACCGGT 1 cut(s) 1995
AspLEI GCGC 8 cut(s) 805, 1330, 1336, 2038, 2356, 2461, 2495, 2497
AspS9I GGNCC 3 cut(s) 1243, 1489, 2114
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 4 cut(s) 322, 826, 1039, 2183
AvaI CYCGRG 2 cut(s) 1050, 1184
AvaII GGWCC 2 cut(s) 1243, 1489
AxyI CCTNAGG 1 cut(s) 1112
BalI TGGCCA 3 cut(s) 891, 927, 2595
BanI GGYRCC 1 cut(s) 2458
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 2 cut(s) 1707, 2620
BbvCI CCTCAGC 3 cut(s) 16, 1817, 2553
BccI CCATC 9 cut(s) 329, 735, 1262, 1673, 1870, 1944, 2129, 2216, 2435
BceAI ACGGC 2 cut(s) 895, 2453
BcgI CGANNNNNNTGC 4 cut(s) 389, 423, 1175, 1209
BciT130I CCWGG 6 cut(s) 929, 1070, 1277, 1497, 1533, 1596
BciVI GTATCC 3 cut(s) 613, 2438, 2518
BclI TGATCA 2 cut(s) 603, 946
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 7 cut(s) 271, 673, 764, 1181, 2060, 2146, 2343
BfaI CTAG 2 cut(s) 326, 1337
BfmI CTRYAG 3 cut(s) 1261, 2118, 2258
BfoI RGCGCY 2 cut(s) 806, 2462
BfuAI ACCTGC 1 cut(s) 1669
BfuI GTATCC 3 cut(s) 613, 2438, 2518
BglII AGATCT 1 cut(s) 1215
BmcAI AGTACT 3 cut(s) 523, 663, 795
Bme1390I CCNGG 7 cut(s) 288, 929, 1070, 1277, 1497, 1533, 1596
Bme18I GGWCC 2 cut(s) 1243, 1489
BmeT110I CYCGRG 2 cut(s) 1050, 1184
BmgT120I GGNCC 3 cut(s) 1243, 1489, 2114
BmiI GGNNCC 3 cut(s) 430, 1119, 2460
BmrFI CCNGG 7 cut(s) 288, 929, 1070, 1277, 1497, 1533, 1596
BmsI GCATC 6 cut(s) 49, 1192, 1354, 2486, 2518, 2612
BpiI GAAGAC 1 cut(s) 180
Bpu10I CCTNAGC 4 cut(s) 16, 1817, 2542, 2553
BpuMI CCSGG 1 cut(s) 288
Bsa29I ATCGAT 1 cut(s) 167
BsaHI GRCGYC 2 cut(s) 2139, 2459
BsaI GGTCTC 2 cut(s) 271, 2060
BsaJI CCNNGG 8 cut(s) 904, 1594, 1914, 2107, 2367, 2564, 2627, 2650
BsaWI WCCGGW 2 cut(s) 1240, 1995
BsaXI ACNNNNNCTCC 4 cut(s) 815, 845, 956, 986
Bsc4I CCNNNNNNNGG 8 cut(s) 287, 331, 920, 1075, 1153, 1984, 2168, 2441
Bse118I RCCGGY 4 cut(s) 1995, 2252, 2455, 2497
Bse1I ACTGG 4 cut(s) 102, 892, 1203, 2237
Bse21I CCTNAGG 1 cut(s) 1112
Bse3DI GCAATG 2 cut(s) 1584, 2001
BseBI CCWGG 6 cut(s) 929, 1070, 1277, 1497, 1533, 1596
BseCI ATCGAT 1 cut(s) 167
BseDI CCNNGG 8 cut(s) 904, 1594, 1914, 2107, 2367, 2564, 2627, 2650
BseGI GGATG 9 cut(s) 219, 340, 746, 1107, 1207, 2140, 2227, 2581, 2646
BseLI CCNNNNNNNGG 8 cut(s) 287, 331, 920, 1075, 1153, 1984, 2168, 2441
BseMI GCAATG 2 cut(s) 1584, 2001
BseNI ACTGG 4 cut(s) 102, 892, 1203, 2237
BsePI GCGCGC 1 cut(s) 2493
BseRI GAGGAG 5 cut(s) 4, 167, 438, 864, 2390
BseYI CCCAGC 2 cut(s) 138, 390
BsgI GTGCAG 1 cut(s) 2048
Bsh1236I CGCG 3 cut(s) 2354, 2493, 2495
Bsh1285I CGRYCG 1 cut(s) 474
BshFI GGCC 8 cut(s) 891, 927, 999, 1283, 2072, 2115, 2468, 2595
BshNI GGYRCC 1 cut(s) 2458
BshTI ACCGGT 1 cut(s) 1995
BshVI ATCGAT 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 474
BsiHKAI GWGCWC 2 cut(s) 1707, 2620
BsiHKCI CYCGRG 2 cut(s) 1050, 1184
BsiSI CCGG 7 cut(s) 288, 1241, 1479, 1996, 2253, 2456, 2498
BslFI GGGAC 1 cut(s) 2417
BslI CCNNNNNNNGG 8 cut(s) 287, 331, 920, 1075, 1153, 1984, 2168, 2441
BsmAI GTCTC 7 cut(s) 271, 673, 764, 1181, 2060, 2146, 2343
BsmBI CGTCTC 3 cut(s) 673, 2146, 2343
BsmFI GGGAC 1 cut(s) 2417
BsmI GAATGC 3 cut(s) 319, 1238, 2279
BsnI GGCC 8 cut(s) 891, 927, 999, 1283, 2072, 2115, 2468, 2595
Bso31I GGTCTC 2 cut(s) 271, 2060
BsoBI CYCGRG 2 cut(s) 1050, 1184
Bsp1286I GDGCHC 2 cut(s) 1707, 2620
Bsp1407I TGTACA 1 cut(s) 2691
Bsp19I CCATGG 1 cut(s) 2627
BspACI CCGC 7 cut(s) 76, 642, 1147, 1938, 1958, 2519, 2608
BspANI GGCC 8 cut(s) 891, 927, 999, 1283, 2072, 2115, 2468, 2595
BspDI ATCGAT 1 cut(s) 167
BspFNI CGCG 3 cut(s) 2354, 2493, 2495
BspLI GGNNCC 3 cut(s) 430, 1119, 2460
BspMAI CTGCAG 2 cut(s) 2122, 2262
BspMI ACCTGC 1 cut(s) 1669
BspPI GGATC 7 cut(s) 643, 1379, 1565, 1853, 2026, 2153, 2326
BspT107I GGYRCC 1 cut(s) 2458
BspTNI GGTCTC 2 cut(s) 271, 2060
BsrDI GCAATG 2 cut(s) 1584, 2001
BsrFI RCCGGY 4 cut(s) 1995, 2252, 2455, 2497
BsrGI TGTACA 1 cut(s) 2691
BsrI ACTGG 4 cut(s) 102, 892, 1203, 2237
BssAI RCCGGY 4 cut(s) 1995, 2252, 2455, 2497
BssECI CCNNGG 8 cut(s) 904, 1594, 1914, 2107, 2367, 2564, 2627, 2650
BssHII GCGCGC 1 cut(s) 2493
BssNI GRCGYC 2 cut(s) 2139, 2459
BssT1I CCWWGG 7 cut(s) 904, 1914, 2107, 2367, 2564, 2627, 2650
Bst2UI CCWGG 6 cut(s) 929, 1070, 1277, 1497, 1533, 1596
Bst4CI ACNGT 7 cut(s) 258, 625, 1265, 1503, 1768, 2062, 2081
Bst6I CTCTTC 2 cut(s) 465, 963
BstACI GRCGYC 2 cut(s) 2139, 2459
BstAPI GCANNNNNTGC 1 cut(s) 1202
BstAUI TGTACA 1 cut(s) 2691
BstC8I GCNNGC 6 cut(s) 532, 1332, 1911, 2034, 2495, 2499
BstDSI CCRYGG 1 cut(s) 2627
BstF5I GGATG 9 cut(s) 219, 340, 746, 1107, 1207, 2140, 2227, 2581, 2646
BstFNI CGCG 3 cut(s) 2354, 2493, 2495
BstH2I RGCGCY 2 cut(s) 806, 2462
BstHHI GCGC 8 cut(s) 805, 1330, 1336, 2038, 2356, 2461, 2495, 2497
BstMAI GTCTC 7 cut(s) 271, 673, 764, 1181, 2060, 2146, 2343
BstMCI CGRYCG 1 cut(s) 474
BstMWI GCNNNNNNNGC 9 cut(s) 133, 888, 1146, 1202, 1598, 2035, 2465, 2474, 2501
BstNI CCWGG 6 cut(s) 929, 1070, 1277, 1497, 1533, 1596
BstNSI RCATGY 2 cut(s) 1334, 1913
BstSCI CCNGG 7 cut(s) 286, 927, 1068, 1275, 1495, 1531, 1594
BstSFI CTRYAG 3 cut(s) 1261, 2118, 2258
BstUI CGCG 3 cut(s) 2354, 2493, 2495
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 2 cut(s) 1215, 2018
BstYI RGATCY 2 cut(s) 1215, 2018
Bsu15I ATCGAT 1 cut(s) 167
Bsu36I CCTNAGG 1 cut(s) 1112
BsuI GTATCC 3 cut(s) 613, 2438, 2518
BsuRI GGCC 8 cut(s) 891, 927, 999, 1283, 2072, 2115, 2468, 2595
BsuTUI ATCGAT 1 cut(s) 167
BtgI CCRYGG 1 cut(s) 2627
BtsCI GGATG 9 cut(s) 219, 340, 746, 1107, 1207, 2140, 2227, 2581, 2646
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 4 cut(s) 336, 1196, 1270, 1773
BveI ACCTGC 1 cut(s) 1669
Cac8I GCNNGC 6 cut(s) 532, 1332, 1911, 2034, 2495, 2499
CfoI GCGC 8 cut(s) 805, 1330, 1336, 2038, 2356, 2461, 2495, 2497
Cfr10I RCCGGY 4 cut(s) 1995, 2252, 2455, 2497
Cfr13I GGNCC 3 cut(s) 1243, 1489, 2114
ClaI ATCGAT 1 cut(s) 167
CseI GACGC 4 cut(s) 1055, 2128, 2360, 2632
CsiI ACCWGGT 1 cut(s) 1275
Csp6I GTAC 9 cut(s) 231, 522, 662, 794, 1351, 1504, 1993, 2041, 2692
CspAI ACCGGT 1 cut(s) 1995
CspCI CAANNNNNGTGG 4 cut(s) 1385, 1420, 2406, 2441
CviQI GTAC 9 cut(s) 231, 522, 662, 794, 1351, 1504, 1993, 2041, 2692
DinI GGCGCC 1 cut(s) 2460
DraI TTTAAA 1 cut(s) 718
DrdI GACNNNNNNGTC 1 cut(s) 1610
DseDI GACNNNNNNGTC 1 cut(s) 1610
EaeI YGGCCR 3 cut(s) 889, 925, 2593
Eam1104I CTCTTC 2 cut(s) 465, 963
EarI CTCTTC 2 cut(s) 465, 963
EciI GGCGGA 1 cut(s) 1136
Eco130I CCWWGG 7 cut(s) 904, 1914, 2107, 2367, 2564, 2627, 2650
Eco147I AGGCCT 1 cut(s) 999
Eco31I GGTCTC 2 cut(s) 271, 2060
Eco32I GATATC 1 cut(s) 1171
Eco47I GGWCC 2 cut(s) 1243, 1489
Eco81I CCTNAGG 1 cut(s) 1112
Eco88I CYCGRG 2 cut(s) 1050, 1184
EcoO109I RGGNCCY 1 cut(s) 2114
EcoRII CCWGG 6 cut(s) 927, 1068, 1275, 1495, 1531, 1594
EcoRV GATATC 1 cut(s) 1171
EcoT14I CCWWGG 7 cut(s) 904, 1914, 2107, 2367, 2564, 2627, 2650
EgeI GGCGCC 1 cut(s) 2460
EheI GGCGCC 1 cut(s) 2460
ErhI CCWWGG 7 cut(s) 904, 1914, 2107, 2367, 2564, 2627, 2650
Esp3I CGTCTC 3 cut(s) 673, 2146, 2343
FalI AAGNNNNNCTT 2 cut(s) 960, 992
FaqI GGGAC 1 cut(s) 2417
FauNDI CATATG 2 cut(s) 84, 2385
FbaI TGATCA 2 cut(s) 603, 946
FblI GTMKAC 2 cut(s) 1614, 2484
FokI GGATG 9 cut(s) 206, 347, 753, 1094, 1214, 2147, 2234, 2588, 2633
FspBI CTAG 2 cut(s) 326, 1337
FspI TGCGCA 1 cut(s) 1329
GlaI GCGC 8 cut(s) 804, 1329, 1335, 2037, 2355, 2460, 2494, 2496
GsaI CCCAGC 2 cut(s) 142, 394
HaeII RGCGCY 2 cut(s) 806, 2462
HaeIII GGCC 8 cut(s) 891, 927, 999, 1283, 2072, 2115, 2468, 2595
HapII CCGG 7 cut(s) 288, 1241, 1479, 1996, 2253, 2456, 2498
HgaI GACGC 4 cut(s) 1055, 2128, 2360, 2632
HhaI GCGC 8 cut(s) 805, 1330, 1336, 2038, 2356, 2461, 2495, 2497
Hin1I GRCGYC 2 cut(s) 2139, 2459
Hin6I GCGC 8 cut(s) 803, 1328, 1334, 2036, 2354, 2459, 2493, 2495
HinP1I GCGC 8 cut(s) 803, 1328, 1334, 2036, 2354, 2459, 2493, 2495
HincII GTYRAC 1 cut(s) 2485
HindII GTYRAC 1 cut(s) 2485
HpaII CCGG 7 cut(s) 288, 1241, 1479, 1996, 2253, 2456, 2498
HphI GGTGA 4 cut(s) 322, 826, 1039, 2183
Hpy166II GTNNAC 4 cut(s) 209, 1413, 1615, 2485
Hpy188III TCNNGA 7 cut(s) 236, 617, 944, 1126, 1184, 1186, 1900
Hpy8I GTNNAC 4 cut(s) 209, 1413, 1615, 2485
HpyAV CCTTC 5 cut(s) 442, 474, 730, 1369, 2573
HpyCH4III ACNGT 7 cut(s) 258, 625, 1265, 1503, 1768, 2062, 2081
HpyF10VI GCNNNNNNNGC 9 cut(s) 133, 888, 1146, 1202, 1598, 2035, 2465, 2474, 2501
Hsp92I GRCGYC 2 cut(s) 2139, 2459
HspAI GCGC 8 cut(s) 803, 1328, 1334, 2036, 2354, 2459, 2493, 2495
KasI GGCGCC 1 cut(s) 2458
KroI GCCGGC 1 cut(s) 2497
KroNI GCCGGC 1 cut(s) 2499
Ksp22I TGATCA 2 cut(s) 603, 946
LmnI GCTCC 5 cut(s) 154, 428, 542, 1987, 2587
LweI GCATC 6 cut(s) 49, 1192, 1354, 2486, 2518, 2612
MabI ACCWGGT 1 cut(s) 1275
MaeI CTAG 2 cut(s) 326, 1337
MaeIII GTNAC 3 cut(s) 215, 1027, 2470
MflI RGATCY 2 cut(s) 1215, 2018
MhlI GDGCHC 2 cut(s) 1707, 2620
MlsI TGGCCA 3 cut(s) 891, 927, 2595
MluNI TGGCCA 3 cut(s) 891, 927, 2595
Mly113I GGCGCC 1 cut(s) 2459
MlyI GAGTC 6 cut(s) 451, 477, 803, 833, 1554, 1779
MmeI TCCRAC 3 cut(s) 91, 801, 1056
Mox20I TGGCCA 3 cut(s) 891, 927, 2595
MroNI GCCGGC 1 cut(s) 2497
MscI TGGCCA 3 cut(s) 891, 927, 2595
MseI TTAA 6 cut(s) 306, 497, 503, 579, 717, 2698
MslI CAYNNNNRTG 2 cut(s) 1562, 2384
Msp20I TGGCCA 3 cut(s) 891, 927, 2595
MspA1I CMGCKG 5 cut(s) 245, 534, 1418, 2249, 2557
MspI CCGG 7 cut(s) 288, 1241, 1479, 1996, 2253, 2456, 2498
MspR9I CCNGG 7 cut(s) 288, 929, 1070, 1277, 1497, 1533, 1596
Mva1269I GAATGC 3 cut(s) 319, 1238, 2279
MvaI CCWGG 6 cut(s) 929, 1070, 1277, 1497, 1533, 1596
MvnI CGCG 3 cut(s) 2354, 2493, 2495
MwoI GCNNNNNNNGC 9 cut(s) 133, 888, 1146, 1202, 1598, 2035, 2465, 2474, 2501
NaeI GCCGGC 1 cut(s) 2499
NarI GGCGCC 1 cut(s) 2459
NciI CCSGG 1 cut(s) 288
NcoI CCATGG 1 cut(s) 2627
NdeI CATATG 2 cut(s) 84, 2385
NgoMIV GCCGGC 1 cut(s) 2497
NlaIV GGNNCC 3 cut(s) 430, 1119, 2460
NmuCI GTSAC 1 cut(s) 1027
NsbI TGCGCA 1 cut(s) 1329
NspI RCATGY 2 cut(s) 1334, 1913
PaeI GCATGC 2 cut(s) 1334, 1913
PaeR7I CTCGAG 2 cut(s) 1050, 1184
PauI GCGCGC 1 cut(s) 2493
PceI AGGCCT 1 cut(s) 999
PctI GAATGC 3 cut(s) 319, 1238, 2279
PdiI GCCGGC 1 cut(s) 2499
PfeI GAWTC 7 cut(s) 283, 595, 1018, 1308, 1358, 1851, 2177
PflMI CCANNNNNTGG 2 cut(s) 1075, 2441
PinAI ACCGGT 1 cut(s) 1995
Ple19I CGATCG 1 cut(s) 474
PleI GAGTC 6 cut(s) 451, 477, 803, 832, 1553, 1779
PluTI GGCGCC 1 cut(s) 2462
PpsI GAGTC 6 cut(s) 451, 477, 803, 832, 1553, 1779
Psp6I CCWGG 6 cut(s) 927, 1068, 1275, 1495, 1531, 1594
PspFI CCCAGC 2 cut(s) 138, 390
PspGI CCWGG 6 cut(s) 927, 1068, 1275, 1495, 1531, 1594
PspN4I GGNNCC 3 cut(s) 430, 1119, 2460
PspPI GGNCC 3 cut(s) 1243, 1489, 2114
PspXI VCTCGAGB 1 cut(s) 1050
PsrI GAACNNNNNNTAC 2 cut(s) 2033, 2065
PstI CTGCAG 2 cut(s) 2122, 2262
PsuI RGATCY 2 cut(s) 1215, 2018
PteI GCGCGC 1 cut(s) 2493
PvuI CGATCG 1 cut(s) 474
PvuII CAGCTG 5 cut(s) 245, 534, 1418, 2249, 2557
RsaI GTAC 9 cut(s) 232, 523, 663, 795, 1352, 1505, 1994, 2042, 2693
RsaNI GTAC 9 cut(s) 231, 522, 662, 794, 1351, 1504, 1993, 2041, 2692
RseI CAYNNNNRTG 2 cut(s) 1562, 2384
SalI GTCGAC 1 cut(s) 2483
SaqAI TTAA 6 cut(s) 306, 497, 503, 579, 717, 2698
Sau96I GGNCC 3 cut(s) 1243, 1489, 2114
ScaI AGTACT 3 cut(s) 523, 663, 795
SchI GAGTC 6 cut(s) 451, 477, 803, 833, 1554, 1779
ScrFI CCNGG 7 cut(s) 288, 929, 1070, 1277, 1497, 1533, 1596
SduI GDGCHC 2 cut(s) 1707, 2620
SexAI ACCWGGT 1 cut(s) 1275
SfaNI GCATC 6 cut(s) 49, 1192, 1354, 2486, 2518, 2612
SfcI CTRYAG 3 cut(s) 1261, 2118, 2258
SfoI GGCGCC 1 cut(s) 2460
Sfr274I CTCGAG 2 cut(s) 1050, 1184
SinI GGWCC 2 cut(s) 1243, 1489
SlaI CTCGAG 2 cut(s) 1050, 1184
SmiMI CAYNNNNRTG 2 cut(s) 1562, 2384
SmlI CTYRAG 2 cut(s) 1050, 1184
SmoI CTYRAG 2 cut(s) 1050, 1184
SphI GCATGC 2 cut(s) 1334, 1913
SseBI AGGCCT 1 cut(s) 999
SsiI CCGC 7 cut(s) 76, 642, 1147, 1938, 1958, 2519, 2608
SspDI GGCGCC 1 cut(s) 2458
SspMI CTAG 2 cut(s) 326, 1337
StuI AGGCCT 1 cut(s) 999
StyD4I CCNGG 7 cut(s) 286, 927, 1068, 1275, 1495, 1531, 1594
StyI CCWWGG 7 cut(s) 904, 1914, 2107, 2367, 2564, 2627, 2650
TaaI ACNGT 7 cut(s) 258, 625, 1265, 1503, 1768, 2062, 2081
TaqII GACCGA 3 cut(s) 295, 1506, 2042
TatI WGTACW 4 cut(s) 521, 661, 793, 2691
TauI GCSGC 3 cut(s) 78, 1961, 2610
TfiI GAWTC 7 cut(s) 283, 595, 1018, 1308, 1358, 1851, 2177
Tru1I TTAA 6 cut(s) 306, 497, 503, 579, 717, 2698
Tru9I TTAA 6 cut(s) 306, 497, 503, 579, 717, 2698
TscAI CASTG 4 cut(s) 336, 1203, 1270, 1773
TseFI GTSAC 1 cut(s) 1027
Tsp45I GTSAC 1 cut(s) 1027
TspGWI ACGGA 3 cut(s) 603, 1600, 2234
TspRI CASTG 4 cut(s) 336, 1203, 1270, 1773
Van91I CCANNNNNTGG 2 cut(s) 1075, 2441
VpaK11BI GGWCC 2 cut(s) 1243, 1489
XapI RAATTY 4 cut(s) 299, 700, 1379, 1822
XceI RCATGY 2 cut(s) 1334, 1913
XhoI CTCGAG 2 cut(s) 1050, 1184
XmiI GTMKAC 2 cut(s) 1614, 2484
XspI CTAG 2 cut(s) 326, 1337
ZrmI AGTACT 3 cut(s) 523, 663, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.