FvH4_5g19160

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011563
Physical Location & Seq
Forward (+)
11051021 .. 11052544
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19160.t1

Sequence Viewer

Length: 798 bp
ATGAACTCGGCTGCTACTGGCTATATTATCATAGATGATAATGGTCAGTTTGTAGTTGCTGGTGCAAAAAGAGTTGGTTCCTCAAGTGTTCCAACAGTTGAAGTTGCTGCTTTAAGGGAAGGACTTCTAAAAGCAAGAGATAAGCACATCAAGATAATGGTAGAAGAAGACTCAAAGTTGGTGATTGATTGCATTAAGGGCACTTGTGCTACCCCTGGGAGACTCAAGAAAATGGTTCAAGACATTAAGCAAATATGCAAATATTTTGAGAGTTGTTGTTTTCAACATGTTTTTCGAGAAGCTAACTTCTCGGCGGATGCCCTTGCAAACTTAGATCACACAAGCAACATGCATATTGACTTGGAAGGATCTTGTCCTCTTCAGATTGGAAATGCTTTATTATTTGACCGCTTGGGGTTAAGACCGTCAGAAATTCAAGGGAAAGAATTCTTGGTCGGGGATGACAAAGGCTGGAGCCCCTATCCCGGCTTATCTGACTGGCCAAACGGCAAGACCTTCACCCCCGGAGATATCTTGGTTTTCAAGCACAACAATGCAAATGCTTCTGTACGATGGTGGGATGATGAAATCAAATATAAGGAATATCCGGATATTTGTTACGCAAAATGTGACAATTTTTTGTGTCCTAACTACAAAGATTTCAACTCGGGAAATGACAATATTGTATTAAAAGAAGGAAAGAACTACTTCGTTTCGCCCGACCACGATCGTTGCGATCAAGGATTTAAGATCAAAGTTTGTGCTGAGTTCAATGGCAAAAGAGCAAGCTGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

266

Amino Acids

29.64

Weight (kDa)

6.09

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 3 - 110 1.4e-20 Reverse transcriptase-like
Cu_bind_like PF02298 164 - 248 3.7e-10 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 607
AciI CCGC 2 cut(s) 314, 409
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 1 cut(s) 500
AcsI RAATTY 2 cut(s) 432, 446
AcuI CTGAAG 1 cut(s) 365
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 1 cut(s) 485
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 7 cut(s) 101, 239, 284, 437, 544, 664, 772
AjnI CCWGG 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AluBI AGCT 2 cut(s) 302, 789
AluI AGCT 2 cut(s) 302, 789
Alw26I GTCTC 1 cut(s) 214
AlwI GGATC 1 cut(s) 376
Ama87I CYCGRG 1 cut(s) 667
Aor13HI TCCGGA 1 cut(s) 607
AoxI GGCC 1 cut(s) 500
ApeKI GCWGC 2 cut(s) 11, 107
ApoI RAATTY 2 cut(s) 432, 446
Asp700I GAANNNNTTC 3 cut(s) 123, 446, 707
AsuC2I CCSGG 2 cut(s) 486, 525
AsuHPI GGTGA 2 cut(s) 193, 511
AvaI CYCGRG 1 cut(s) 667
BaeGI GKGCMC 1 cut(s) 203
BalI TGGCCA 1 cut(s) 502
BanII GRGCYC 1 cut(s) 479
BbsI GAAGAC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 94
BccI CCATC 1 cut(s) 567
BceAI ACGGC 1 cut(s) 523
BciT130I CCWGG 1 cut(s) 216
BcnI CCSGG 2 cut(s) 486, 525
BcoDI GTCTC 1 cut(s) 214
BisI GCNGC 2 cut(s) 12, 108
BlsI GCNGC 2 cut(s) 13, 109
Bme1390I CCNGG 3 cut(s) 216, 486, 525
BmeT110I CYCGRG 1 cut(s) 667
BmiI GGNNCC 2 cut(s) 79, 476
BmrFI CCNGG 3 cut(s) 216, 486, 525
BmsI GCATC 1 cut(s) 307
BpiI GAAGAC 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 493
BpuEI CTTGAG 2 cut(s) 67, 209
BpuMI CCSGG 2 cut(s) 486, 525
BsaJI CCNNGG 3 cut(s) 214, 215, 523
BsaWI WCCGGW 1 cut(s) 607
Bsc4I CCNNNNNNNGG 1 cut(s) 485
Bse1I ACTGG 2 cut(s) 22, 503
BseAI TCCGGA 1 cut(s) 607
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 3 cut(s) 214, 215, 523
BseGI GGATG 3 cut(s) 322, 466, 586
BseLI CCNNNNNNNGG 1 cut(s) 485
BseMII CTCAG 1 cut(s) 756
BseNI ACTGG 2 cut(s) 22, 503
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 1 cut(s) 94
Bsh1285I CGRYCG 1 cut(s) 730
BshFI GGCC 1 cut(s) 502
BsiEI CGRYCG 1 cut(s) 730
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 3 cut(s) 486, 525, 608
BslI CCNNNNNNNGG 1 cut(s) 485
BsmAI GTCTC 1 cut(s) 214
BsnI GGCC 1 cut(s) 502
BsoBI CYCGRG 1 cut(s) 667
Bsp1286I GDGCHC 2 cut(s) 203, 479
Bsp13I TCCGGA 1 cut(s) 607
Bsp143I GATC 5 cut(s) 334, 368, 727, 736, 750
BspACI CCGC 2 cut(s) 314, 409
BspANI GGCC 1 cut(s) 502
BspCNI CTCAG 1 cut(s) 757
BspEI TCCGGA 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 79, 476
BspPI GGATC 1 cut(s) 376
BsrI ACTGG 2 cut(s) 22, 503
BssECI CCNNGG 3 cut(s) 214, 215, 523
BssMI GATC 5 cut(s) 334, 368, 727, 736, 750
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 2 cut(s) 97, 426
Bst6I CTCTTC 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 787
BstDEI CTNAG 2 cut(s) 331, 765
BstF5I GGATG 3 cut(s) 322, 466, 586
BstKTI GATC 5 cut(s) 337, 371, 730, 739, 753
BstMAI GTCTC 1 cut(s) 214
BstMBI GATC 5 cut(s) 334, 368, 727, 736, 750
BstMCI CGRYCG 1 cut(s) 730
BstMWI GCNNNNNNNGC 1 cut(s) 198
BstNI CCWGG 1 cut(s) 216
BstNSI RCATGY 2 cut(s) 290, 352
BstSCI CCNGG 3 cut(s) 214, 484, 523
BstSLI GKGCMC 1 cut(s) 203
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 1 cut(s) 174
BstX2I RGATCY 1 cut(s) 368
BstYI RGATCY 1 cut(s) 368
BsuRI GGCC 1 cut(s) 502
BtsCI GGATG 3 cut(s) 322, 466, 586
Cac8I GCNNGC 1 cut(s) 787
Csp6I GTAC 1 cut(s) 569
CspCI CAANNNNNGTGG 2 cut(s) 713, 748
CviAII CATG 2 cut(s) 287, 349
CviJI RGCY 8 cut(s) 11, 21, 302, 471, 477, 489, 502, 789
CviKI_1 RGCY 8 cut(s) 11, 21, 302, 471, 477, 489, 502, 789
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 2 cut(s) 331, 765
DpnI GATC 5 cut(s) 336, 370, 729, 738, 752
DpnII GATC 5 cut(s) 334, 368, 727, 736, 750
EaeI YGGCCR 1 cut(s) 500
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
EciI GGCGGA 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 479
Eco32I GATATC 1 cut(s) 532
Eco57I CTGAAG 1 cut(s) 365
Eco88I CYCGRG 1 cut(s) 667
EcoRI GAATTC 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 214
EcoRV GATATC 1 cut(s) 532
EcoT22I ATGCAT 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 479
FaeI CATG 2 cut(s) 290, 352
FaiI YATR 7 cut(s) 24, 32, 256, 288, 350, 354, 597
FalI AAGNNNNNCTT 2 cut(s) 692, 724
FatI CATG 2 cut(s) 286, 348
Fnu4HI GCNGC 2 cut(s) 12, 108
FokI GGATG 3 cut(s) 329, 473, 593
FriOI GRGCYC 1 cut(s) 479
Fsp4HI GCNGC 2 cut(s) 12, 108
GluI GCNGC 2 cut(s) 12, 108
GsuI CTGGAG 1 cut(s) 493
HaeIII GGCC 1 cut(s) 502
HapII CCGG 3 cut(s) 486, 525, 608
Hin1II CATG 2 cut(s) 290, 352
HinfI GANTC 2 cut(s) 170, 222
HpaII CCGG 3 cut(s) 486, 525, 608
HphI GGTGA 2 cut(s) 193, 511
Hpy188I TCNGA 3 cut(s) 384, 430, 496
Hpy188III TCNNGA 6 cut(s) 151, 226, 239, 296, 608, 669
HpyAV CCTTC 4 cut(s) 113, 359, 526, 689
HpyCH4III ACNGT 2 cut(s) 97, 426
HpyCH4V TGCA 6 cut(s) 65, 192, 258, 326, 352, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 198
HpyF3I CTNAG 2 cut(s) 331, 765
Hsp92II CATG 2 cut(s) 290, 352
Kpn2I TCCGGA 1 cut(s) 607
Kzo9I GATC 5 cut(s) 334, 368, 727, 736, 750
LmnI GCTCC 1 cut(s) 474
LpnPI CCDG 9 cut(s) 3, 45, 201, 228, 457, 484, 499, 538, 621
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 1 cut(s) 307
MaeIII GTNAC 2 cut(s) 617, 629
MalI GATC 5 cut(s) 336, 370, 729, 738, 752
MboI GATC 5 cut(s) 334, 368, 727, 736, 750
MboII GAAGA 3 cut(s) 176, 179, 371
MflI RGATCY 1 cut(s) 368
MhlI GDGCHC 2 cut(s) 203, 479
MlsI TGGCCA 1 cut(s) 502
MluCI AATT 4 cut(s) 432, 446, 634, 793
MluNI TGGCCA 1 cut(s) 502
MlyI GAGTC 2 cut(s) 164, 216
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 2 cut(s) 91, 387
Mox20I TGGCCA 1 cut(s) 502
Mph1103I ATGCAT 1 cut(s) 354
MroI TCCGGA 1 cut(s) 607
MroXI GAANNNNTTC 3 cut(s) 123, 446, 707
MscI TGGCCA 1 cut(s) 502
MseI TTAA 7 cut(s) 113, 195, 246, 419, 689, 747, 796
MslI CAYNNNNRTG 1 cut(s) 552
Msp20I TGGCCA 1 cut(s) 502
MspI CCGG 3 cut(s) 486, 525, 608
MspR9I CCNGG 3 cut(s) 216, 486, 525
MvaI CCWGG 1 cut(s) 216
MwoI GCNNNNNNNGC 1 cut(s) 198
NciI CCSGG 2 cut(s) 486, 525
NdeII GATC 5 cut(s) 334, 368, 727, 736, 750
NlaIII CATG 2 cut(s) 290, 352
NlaIV GGNNCC 2 cut(s) 79, 476
NmeAIII GCCGAG 1 cut(s) 290
NmuCI GTSAC 1 cut(s) 629
NsiI ATGCAT 1 cut(s) 354
NspI RCATGY 2 cut(s) 290, 352
PasI CCCWGGG 1 cut(s) 215
PciI ACATGT 1 cut(s) 286
PcsI WCGNNNNNNNCGW 2 cut(s) 717, 732
PdmI GAANNNNTTC 3 cut(s) 123, 446, 707
PkrI GCNGC 2 cut(s) 13, 109
Ple19I CGATCG 1 cut(s) 730
PleI GAGTC 2 cut(s) 164, 216
PpsI GAGTC 2 cut(s) 164, 216
PscI ACATGT 1 cut(s) 286
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 2 cut(s) 79, 476
PsuI RGATCY 1 cut(s) 368
PvuI CGATCG 1 cut(s) 730
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
RseI CAYNNNNRTG 1 cut(s) 552
SaqAI TTAA 7 cut(s) 113, 195, 246, 419, 689, 747, 796
SatI GCNGC 2 cut(s) 12, 108
Sau3AI GATC 5 cut(s) 334, 368, 727, 736, 750
SchI GAGTC 2 cut(s) 164, 216
ScrFI CCNGG 3 cut(s) 216, 486, 525
SduI GDGCHC 2 cut(s) 203, 479
SetI ASST 3 cut(s) 304, 518, 791
SfaNI GCATC 1 cut(s) 307
SmiMI CAYNNNNRTG 1 cut(s) 552
SmlI CTYRAG 2 cut(s) 82, 224
SmoI CTYRAG 2 cut(s) 82, 224
Sse9I AATT 4 cut(s) 432, 446, 634, 793
SsiI CCGC 2 cut(s) 314, 409
SspI AATATT 2 cut(s) 263, 682
StyD4I CCNGG 3 cut(s) 214, 484, 523
TaaI ACNGT 2 cut(s) 97, 426
TaqI TCGA 1 cut(s) 295
TasI AATT 4 cut(s) 432, 446, 634, 793
Tru1I TTAA 7 cut(s) 113, 195, 246, 419, 689, 747, 796
Tru9I TTAA 7 cut(s) 113, 195, 246, 419, 689, 747, 796
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 2 cut(s) 11, 107
Tsp45I GTSAC 1 cut(s) 629
TspDTI ATGAA 2 cut(s) 17, 600
XapI RAATTY 2 cut(s) 432, 446
XceI RCATGY 2 cut(s) 290, 352
XmnI GAANNNNTTC 3 cut(s) 123, 446, 707
Zsp2I ATGCAT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.