FvH4_5g19322

Domain of unknown function (DUF4219)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009688
Physical Location & Seq
Reverse (-)
11186458 .. 11190260
3803 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19322.t1

Sequence Viewer

Length: 750 bp
ATGACAAGTGCTGTACCGGATGCCATTGTTCTTAAACTTCTAAACGTGGATAATTATGACCATTGGAGTCTTCGTGTTAAGACCTACTTGTTAGCTGAAGGTCTTTGGAAGGTTGTTGAATCCACCGGTGATCCACCTAAACCAGAAGATGATGAAGAAAAGTTTGATGCATGGAGTAAGCAGAATGCAAAAGCTCTACATGTAATCCATATTTCTTGCGGGTCTCGTATTTTTTCACTTATTAGTGATACCACGAGTGCCAGAGAAGCCTGGGATCTGTTGGCAAAAAAGTTAAAAACCGACTACATAGATGAGATGTCTGATTTTCCCCTGCCAGTTGATGGTGGCAGGCGCGTGCATGATGGAAACATAAAAGAGGAATATGCGGCTGATGGGACGGCACTGTTGCCGGATTCGGGTCGAGCAAGTCGGAGCGGGGCAGCAGACACGTGGTCAGGGAGGGGACCCGGGGCTGGGTGGTGCGACGCTGTGGTGGTTCACTCACCGTTCTGGGTTAAAGGCGCATTGGAGCAGGACAACCGGATCGGTGGTCCGAGCGGTGCACTGCCGGAATCTGGGCTGGGAGAGTTGCGCGCCGTCGGGGCAGTGGCGGCGAGGCGGGAGATCGCCGGGAAAGGAGGCCGACTAGGGCTCGACCAGGGATGGGTCAAGGCTGCTGGGCTTCGACCGAGGTGCTGGGGCTGCCGTGGTGGAGGTAGCCGACGTCGCTCAGGGAAGGTGGGTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4219

Protein Analysis

250

Amino Acids

26.69

Weight (kDa)

7.69

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 14 - 40 2.3e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 27 - 97 4.7e-11 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 727
AccB1I GGYRCC 1 cut(s) 743
AccB7I CCANNNNNTGG 1 cut(s) 341
AccBSI CCGCTC 2 cut(s) 435, 558
AccII CGCG 2 cut(s) 354, 594
AciI CCGC 6 cut(s) 219, 386, 435, 558, 611, 619
AclWI GGATC 3 cut(s) 125, 282, 551
AcuI CTGAAG 1 cut(s) 117
AcvI CACGTG 1 cut(s) 450
AcyI GRCGYC 1 cut(s) 724
AfaI GTAC 1 cut(s) 15
AfiI CCNNNNNNNGG 6 cut(s) 341, 416, 473, 474, 575, 664
AflIII ACRYGT 2 cut(s) 199, 447
AgeI ACCGGT 1 cut(s) 125
AgsI TTSAA 1 cut(s) 119
AhdI GACNNNNNGTC 1 cut(s) 451
AjnI CCWGG 2 cut(s) 269, 657
AluBI AGCT 2 cut(s) 95, 194
AluI AGCT 2 cut(s) 95, 194
Alw21I GWGCWC 1 cut(s) 565
Alw26I GTCTC 1 cut(s) 228
Alw44I GTGCAC 1 cut(s) 561
AlwI GGATC 3 cut(s) 125, 282, 551
Ama87I CYCGRG 1 cut(s) 467
AoxI GGCC 1 cut(s) 640
ApaLI GTGCAC 1 cut(s) 561
ApeKI GCWGC 3 cut(s) 440, 674, 702
AsiGI ACCGGT 1 cut(s) 125
AspLEI GCGC 4 cut(s) 354, 524, 594, 596
AspS9I GGNCC 2 cut(s) 464, 551
AsuC2I CCSGG 3 cut(s) 468, 469, 631
AsuHPI GGTGA 2 cut(s) 140, 495
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 2 cut(s) 464, 551
BaeGI GKGCMC 1 cut(s) 565
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 1 cut(s) 654
BauI CACGAG 1 cut(s) 253
BbrPI CACGTG 1 cut(s) 450
BbsI GAAGAC 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 565
BbvI GCAGC 3 cut(s) 452, 661, 689
BccI CCATC 4 cut(s) 335, 356, 386, 657
BceAI ACGGC 3 cut(s) 414, 581, 690
BcgI CGANNNNNNTGC 2 cut(s) 675, 709
BciT130I CCWGG 2 cut(s) 271, 659
BcnI CCSGG 3 cut(s) 468, 469, 631
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 2 cut(s) 647, 748
BglI GCCNNNNNGGC 1 cut(s) 602
BisI GCNGC 5 cut(s) 387, 441, 612, 675, 703
BlsI GCNGC 5 cut(s) 388, 442, 613, 676, 704
Bme1390I CCNGG 5 cut(s) 271, 468, 469, 631, 659
Bme18I GGWCC 2 cut(s) 464, 551
BmeRI GACNNNNNGTC 1 cut(s) 451
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 464, 551
BmiI GGNNCC 3 cut(s) 465, 466, 745
BmrFI CCNGG 5 cut(s) 271, 468, 469, 631, 659
BmsI GCATC 2 cut(s) 10, 157
BpiI GAAGAC 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 730
BpuMI CCSGG 3 cut(s) 468, 469, 631
BsaAI YACGTR 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 724
BsaI GGTCTC 1 cut(s) 228
BsaJI CCNNGG 6 cut(s) 270, 467, 468, 658, 689, 706
BsaWI WCCGGW 3 cut(s) 16, 125, 540
BsaXI ACNNNNNCTCC 2 cut(s) 58, 88
Bsc4I CCNNNNNNNGG 6 cut(s) 341, 416, 473, 474, 575, 664
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 335
BseBI CCWGG 2 cut(s) 271, 659
BseDI CCNNGG 6 cut(s) 270, 467, 468, 658, 689, 706
BseGI GGATG 2 cut(s) 25, 668
BseLI CCNNNNNNNGG 6 cut(s) 341, 416, 473, 474, 575, 664
BseMII CTCAG 1 cut(s) 744
BseNI ACTGG 1 cut(s) 335
BsePI GCGCGC 1 cut(s) 592
BseSI GKGCMC 1 cut(s) 565
BseXI GCAGC 3 cut(s) 452, 661, 689
BseYI CCCAGC 4 cut(s) 473, 580, 677, 696
Bsh1236I CGCG 2 cut(s) 354, 594
Bsh1285I CGRYCG 1 cut(s) 689
BshFI GGCC 1 cut(s) 642
BshNI GGYRCC 1 cut(s) 743
BshTI ACCGGT 1 cut(s) 125
BsiEI CGRYCG 1 cut(s) 689
BsiHKAI GWGCWC 1 cut(s) 565
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 7 cut(s) 17, 126, 410, 468, 541, 569, 630
BslFI GGGAC 2 cut(s) 409, 477
BslI CCNNNNNNNGG 6 cut(s) 341, 416, 473, 474, 575, 664
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 2 cut(s) 409, 477
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 1 cut(s) 642
Bso31I GGTCTC 1 cut(s) 228
BsoBI CYCGRG 1 cut(s) 467
Bsp1286I GDGCHC 2 cut(s) 565, 654
Bsp143I GATC 4 cut(s) 130, 274, 543, 624
BspACI CCGC 6 cut(s) 219, 386, 435, 558, 611, 619
BspANI GGCC 1 cut(s) 642
BspCNI CTCAG 1 cut(s) 743
BspFNI CGCG 2 cut(s) 354, 594
BspLI GGNNCC 3 cut(s) 465, 466, 745
BspPI GGATC 3 cut(s) 125, 282, 551
BspT107I GGYRCC 1 cut(s) 743
BspTNI GGTCTC 1 cut(s) 228
BsrBI CCGCTC 2 cut(s) 435, 558
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 1 cut(s) 335
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 6 cut(s) 270, 467, 468, 658, 689, 706
BssHII GCGCGC 1 cut(s) 592
BssMI GATC 4 cut(s) 130, 274, 543, 624
BssNI GRCGYC 1 cut(s) 724
BssSI CACGAG 1 cut(s) 253
Bst2BI CACGAG 1 cut(s) 253
Bst2UI CCWGG 2 cut(s) 271, 659
Bst4CI ACNGT 2 cut(s) 405, 507
BstACI GRCGYC 1 cut(s) 724
BstBAI YACGTR 1 cut(s) 450
BstC8I GCNNGC 3 cut(s) 350, 356, 594
BstDEI CTNAG 1 cut(s) 730
BstDSI CCRYGG 1 cut(s) 706
BstF5I GGATG 2 cut(s) 25, 668
BstFNI CGCG 2 cut(s) 354, 594
BstHHI GCGC 4 cut(s) 354, 524, 594, 596
BstKTI GATC 4 cut(s) 133, 277, 546, 627
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 4 cut(s) 130, 274, 543, 624
BstMCI CGRYCG 1 cut(s) 689
BstMWI GCNNNNNNNGC 5 cut(s) 266, 602, 611, 702, 726
BstNI CCWGG 2 cut(s) 271, 659
BstNSI RCATGY 1 cut(s) 203
BstSCI CCNGG 5 cut(s) 269, 466, 467, 629, 657
BstSLI GKGCMC 1 cut(s) 565
BstUI CGCG 2 cut(s) 354, 594
BstV1I GCAGC 3 cut(s) 452, 661, 689
BstV2I GAAGAC 1 cut(s) 62
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 1 cut(s) 642
BtgI CCRYGG 1 cut(s) 706
BtsCI GGATG 2 cut(s) 25, 668
BtsI GCAGTG 2 cut(s) 563, 612
BtsIMutI CAGTG 3 cut(s) 401, 563, 612
Cac8I GCNNGC 3 cut(s) 350, 356, 594
CfoI GCGC 4 cut(s) 354, 524, 594, 596
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 464, 551
Cfr9I CCCGGG 1 cut(s) 467
CseI GACGC 1 cut(s) 494
Csp6I GTAC 1 cut(s) 14
CspAI ACCGGT 1 cut(s) 125
CviAII CATG 3 cut(s) 171, 200, 359
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 1 cut(s) 730
DpnI GATC 4 cut(s) 132, 276, 545, 626
DpnII GATC 4 cut(s) 130, 274, 543, 624
DriI GACNNNNNGTC 1 cut(s) 451
Eam1105I GACNNNNNGTC 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 654
Eco31I GGTCTC 1 cut(s) 228
Eco47I GGWCC 2 cut(s) 464, 551
Eco57I CTGAAG 1 cut(s) 117
Eco72I CACGTG 1 cut(s) 450
Eco88I CYCGRG 1 cut(s) 467
EcoO109I RGGNCCY 1 cut(s) 464
EcoRII CCWGG 2 cut(s) 269, 657
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 654
FaeI CATG 3 cut(s) 174, 203, 362
FaiI YATR 8 cut(s) 57, 172, 201, 210, 308, 360, 371, 384
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FaqI GGGAC 2 cut(s) 409, 477
FatI CATG 3 cut(s) 170, 199, 358
FauI CCCGC 3 cut(s) 212, 428, 612
Fnu4HI GCNGC 5 cut(s) 387, 441, 612, 675, 703
FokI GGATG 2 cut(s) 32, 675
FriOI GRGCYC 1 cut(s) 654
Fsp4HI GCNGC 5 cut(s) 387, 441, 612, 675, 703
FspBI CTAG 2 cut(s) 647, 748
GlaI GCGC 4 cut(s) 353, 523, 593, 595
GluI GCNGC 5 cut(s) 387, 441, 612, 675, 703
GsaI CCCAGC 4 cut(s) 477, 584, 681, 700
HaeIII GGCC 1 cut(s) 642
HapII CCGG 7 cut(s) 17, 126, 410, 468, 541, 569, 630
HgaI GACGC 1 cut(s) 494
HhaI GCGC 4 cut(s) 354, 524, 594, 596
Hin1I GRCGYC 1 cut(s) 724
Hin1II CATG 3 cut(s) 174, 203, 362
Hin6I GCGC 4 cut(s) 352, 522, 592, 594
HinP1I GCGC 4 cut(s) 352, 522, 592, 594
HinfI GANTC 4 cut(s) 67, 119, 413, 572
HpaII CCGG 7 cut(s) 17, 126, 410, 468, 541, 569, 630
HphI GGTGA 2 cut(s) 140, 495
Hpy166II GTNNAC 2 cut(s) 499, 563
Hpy188I TCNGA 3 cut(s) 322, 432, 555
Hpy8I GTNNAC 2 cut(s) 499, 563
Hpy99I CGWCG 4 cut(s) 488, 602, 726, 729
HpyAV CCTTC 3 cut(s) 92, 103, 730
HpyCH4III ACNGT 2 cut(s) 405, 507
HpyCH4IV ACGT 3 cut(s) 45, 449, 724
HpyCH4V TGCA 4 cut(s) 170, 188, 358, 563
HpyF10VI GCNNNNNNNGC 5 cut(s) 266, 602, 611, 702, 726
HpyF3I CTNAG 1 cut(s) 730
HpySE526I ACGT 3 cut(s) 45, 449, 724
Hsp92I GRCGYC 1 cut(s) 724
Hsp92II CATG 3 cut(s) 174, 203, 362
HspAI GCGC 4 cut(s) 352, 522, 592, 594
KflI GGGWCCC 1 cut(s) 464
Kzo9I GATC 4 cut(s) 130, 274, 543, 624
LmnI GCTCC 2 cut(s) 432, 529
Lsp1109I GCAGC 3 cut(s) 452, 661, 689
LweI GCATC 2 cut(s) 10, 157
MaeI CTAG 2 cut(s) 647, 748
MaeII ACGT 3 cut(s) 45, 449, 724
MalI GATC 4 cut(s) 132, 276, 545, 626
MbiI CCGCTC 2 cut(s) 435, 558
MboI GATC 4 cut(s) 130, 274, 543, 624
MboII GAAGA 3 cut(s) 62, 158, 167
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 2 cut(s) 565, 654
MluCI AATT 1 cut(s) 52
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 6 cut(s) 370, 453, 609, 632, 684, 707
Mph1103I ATGCAT 1 cut(s) 172
MseI TTAA 4 cut(s) 33, 78, 293, 516
MspI CCGG 7 cut(s) 17, 126, 410, 468, 541, 569, 630
MspR9I CCNGG 5 cut(s) 271, 468, 469, 631, 659
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 2 cut(s) 271, 659
MvnI CGCG 2 cut(s) 354, 594
MwoI GCNNNNNNNGC 5 cut(s) 266, 602, 611, 702, 726
NciI CCSGG 3 cut(s) 468, 469, 631
NdeII GATC 4 cut(s) 130, 274, 543, 624
NlaIII CATG 3 cut(s) 174, 203, 362
NlaIV GGNNCC 3 cut(s) 465, 466, 745
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 203
PauI GCGCGC 1 cut(s) 592
PciI ACATGT 1 cut(s) 199
PctI GAATGC 1 cut(s) 190
PfeI GAWTC 3 cut(s) 119, 413, 572
PflMI CCANNNNNTGG 1 cut(s) 341
PinAI ACCGGT 1 cut(s) 125
PkrI GCNGC 5 cut(s) 388, 442, 613, 676, 704
PleI GAGTC 1 cut(s) 75
PmaCI CACGTG 1 cut(s) 450
PmlI CACGTG 1 cut(s) 450
PpsI GAGTC 1 cut(s) 75
Ppu21I YACGTR 1 cut(s) 450
PpuMI RGGWCCY 1 cut(s) 464
PscI ACATGT 1 cut(s) 199
Psp5II RGGWCCY 1 cut(s) 464
Psp6I CCWGG 2 cut(s) 269, 657
PspCI CACGTG 1 cut(s) 450
PspFI CCCAGC 4 cut(s) 473, 580, 677, 696
PspGI CCWGG 2 cut(s) 269, 657
PspN4I GGNNCC 3 cut(s) 465, 466, 745
PspPI GGNCC 2 cut(s) 464, 551
PspPPI RGGWCCY 1 cut(s) 464
PsuI RGATCY 1 cut(s) 274
PteI GCGCGC 1 cut(s) 592
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
SaqAI TTAA 4 cut(s) 33, 78, 293, 516
SatI GCNGC 5 cut(s) 387, 441, 612, 675, 703
Sau3AI GATC 4 cut(s) 130, 274, 543, 624
Sau96I GGNCC 2 cut(s) 464, 551
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 5 cut(s) 271, 468, 469, 631, 659
SduI GDGCHC 2 cut(s) 565, 654
SfaNI GCATC 2 cut(s) 10, 157
SgrAI CRCCGGYG 1 cut(s) 125
SinI GGWCC 2 cut(s) 464, 551
SmaI CCCGGG 1 cut(s) 469
Sse9I AATT 1 cut(s) 52
SsiI CCGC 6 cut(s) 219, 386, 435, 558, 611, 619
SspMI CTAG 2 cut(s) 647, 748
StyD4I CCNGG 5 cut(s) 269, 466, 467, 629, 657
TaaI ACNGT 2 cut(s) 405, 507
TaiI ACGT 3 cut(s) 48, 452, 727
TaqI TCGA 3 cut(s) 421, 654, 685
TaqII GACCGA 1 cut(s) 703
TasI AATT 1 cut(s) 52
TauI GCSGC 2 cut(s) 389, 614
TfiI GAWTC 3 cut(s) 119, 413, 572
Tru1I TTAA 4 cut(s) 33, 78, 293, 516
Tru9I TTAA 4 cut(s) 33, 78, 293, 516
TscAI CASTG 3 cut(s) 408, 570, 612
TseI GCWGC 3 cut(s) 440, 674, 702
TspDTI ATGAA 1 cut(s) 168
TspMI CCCGGG 1 cut(s) 467
TspRI CASTG 3 cut(s) 408, 570, 612
Van91I CCANNNNNTGG 1 cut(s) 341
VneI GTGCAC 1 cut(s) 561
VpaK11BI GGWCC 2 cut(s) 464, 551
XceI RCATGY 1 cut(s) 203
XmaI CCCGGG 1 cut(s) 467
XspI CTAG 2 cut(s) 647, 748
ZraI GACGTC 1 cut(s) 725
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.