pycom11g04720

Domain of unknown function (DUF4219)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009688
Physical Location & Seq
Forward (+)
3628479 .. 3628813
335 bp
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UTR
Exon/CDS
Intron
pycom11g04720.1

Sequence Viewer

Length: 282 bp
ATGGGTGATCTTCAAGTTGTTGGAGGAATCAAGAAGCTCAACAACCAAAACTATAACACGTGGGCAACGTGTATAGAGTCTTACCTTCAAGGTCAAGACTTGTGGGAGGTTGTCGGTGGTAGTGAAGTTACACAACCGGCAGCGGAAGATGTTAACGGCGTCTTGCGGAAGTGGAAAATTAAAGCAGGCAAATCAATGTTTGCCTTAAAAACCACAATAGAAGAAGAAATGTTGGAGCACATTCGGGATGAACGATACAAAATTGCAACTTCTCGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4219 Retrotran_gag_2 zf-CCHC

Protein Analysis

94

Amino Acids

10.56

Weight (kDa)

5.44

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 13 - 38 2.7e-12 Domain of unknown function (DUF4219)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 143, 166
AcvI CACGTG 1 cut(s) 60
AcyI GRCGYC 1 cut(s) 159
AflIII ACRYGT 2 cut(s) 57, 68
AgsI TTSAA 2 cut(s) 14, 89
AluBI AGCT 1 cut(s) 37
AluI AGCT 1 cut(s) 37
Alw21I GWGCWC 1 cut(s) 240
Ama87I CYCGRG 1 cut(s) 273
ApeKI GCWGC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 273
BbrPI CACGTG 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 152
BceAI ACGGC 1 cut(s) 172
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmeT110I CYCGRG 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 159
Bse118I RCCGGY 1 cut(s) 136
BseGI GGATG 1 cut(s) 253
BseXI GCAGC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 1 cut(s) 137
BsoBI CYCGRG 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 1 cut(s) 7
BspACI CCGC 2 cut(s) 143, 166
BsrFI RCCGGY 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 136
BssMI GATC 1 cut(s) 7
BssNI GRCGYC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 159
BstBAI YACGTR 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 187
BstF5I GGATG 1 cut(s) 253
BstKTI GATC 1 cut(s) 10
BstMBI GATC 1 cut(s) 7
BstV1I GCAGC 1 cut(s) 152
BtsCI GGATG 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 187
Cfr10I RCCGGY 1 cut(s) 136
CseI GACGC 1 cut(s) 148
CspCI CAANNNNNGTGG 2 cut(s) 83, 118
CviJI RGCY 1 cut(s) 37
CviKI_1 RGCY 1 cut(s) 37
DpnI GATC 1 cut(s) 9
DpnII GATC 1 cut(s) 7
Eco72I CACGTG 1 cut(s) 60
Eco88I CYCGRG 1 cut(s) 273
FaiI YATR 2 cut(s) 54, 74
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 1 cut(s) 141
GluI GCNGC 1 cut(s) 141
HapII CCGG 1 cut(s) 137
HgaI GACGC 1 cut(s) 148
Hin1I GRCGYC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 2 cut(s) 27, 77
HpaI GTTAAC 1 cut(s) 154
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 1 cut(s) 154
Hpy188III TCNNGA 5 cut(s) 31, 95, 245, 273, 275
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 1 cut(s) 95
HpyCH4IV ACGT 2 cut(s) 59, 68
HpyCH4V TGCA 1 cut(s) 266
HpySE526I ACGT 2 cut(s) 59, 68
Hsp92I GRCGYC 1 cut(s) 159
KspAI GTTAAC 1 cut(s) 154
Kzo9I GATC 1 cut(s) 7
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 2 cut(s) 150, 171
Lsp1109I GCAGC 1 cut(s) 152
MaeII ACGT 2 cut(s) 59, 68
MaeIII GTNAC 1 cut(s) 127
MalI GATC 1 cut(s) 9
MboI GATC 1 cut(s) 7
MboII GAAGA 3 cut(s) 158, 233, 236
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 2 cut(s) 177, 261
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 2 cut(s) 17, 100
MseI TTAA 3 cut(s) 153, 180, 206
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 1 cut(s) 137
NdeII GATC 1 cut(s) 7
PaeR7I CTCGAG 1 cut(s) 273
PcsI WCGNNNNNNNCGW 2 cut(s) 65, 250
PfeI GAWTC 1 cut(s) 27
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 85
PmaCI CACGTG 1 cut(s) 60
PmlI CACGTG 1 cut(s) 60
PpsI GAGTC 1 cut(s) 85
Ppu21I YACGTR 1 cut(s) 60
PspCI CACGTG 1 cut(s) 60
SaqAI TTAA 3 cut(s) 153, 180, 206
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 1 cut(s) 7
SchI GAGTC 1 cut(s) 86
SduI GDGCHC 1 cut(s) 240
SetI ASST 6 cut(s) 39, 62, 71, 87, 94, 111
Sfr274I CTCGAG 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 273
SmoI CTYRAG 1 cut(s) 273
Sse9I AATT 2 cut(s) 177, 261
SsiI CCGC 2 cut(s) 143, 166
TaiI ACGT 2 cut(s) 62, 71
TaqI TCGA 1 cut(s) 274
TasI AATT 2 cut(s) 177, 261
TfiI GAWTC 1 cut(s) 27
Tru1I TTAA 3 cut(s) 153, 180, 206
Tru9I TTAA 3 cut(s) 153, 180, 206
TseI GCWGC 1 cut(s) 140
TspDTI ATGAA 1 cut(s) 264
XhoI CTCGAG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.