Rroxscaffold_4G00308650

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009688
Physical Location & Seq
Forward (+)
30207333 .. 30207707
375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00308650.1

Sequence Viewer

Length: 375 bp
ATGTTTCGTCTTCTTCCTCTCCCCGTCACCGACCATGGAGCAGAAGAAGGAGAAAGAGGTCTGCGGAACAGTGATGGATCTGCAGCGTTGCCGAAGATTTCTTCAATTCAACTATTGATAGCTAGCATGGCAAGTACTCCAGTTGGCTCTAATGCACTTATTCTGGAAGTTCTTGACCGTAGTAACTATGAAGATTGGAGATCGCGATTGAAAACTTATTTGTTGGCTGAAGATCTTTGGGAGGCTGTCCAAGCAATCGTGAAACCTCCGAACGTGAAGATGATCAAGCCGATTATAAGGCCTAGACCAAAAAGAATGCCAAGGCTTTGTACGCAATCCAAAATACATGCGGGAGAGAGCTGTTCCCTTTTATAA

Protein Analysis

124

Amino Acids

13.79

Weight (kDa)

9.34

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 58 - 83 9.8e-09 Domain of unknown function (DUF4219)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 296, 373
AccII CGCG 1 cut(s) 205
AciI CCGC 2 cut(s) 64, 350
AclWI GGATC 1 cut(s) 85
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 2 cut(s) 136, 331
AgsI TTSAA 3 cut(s) 105, 110, 211
AluBI AGCT 2 cut(s) 122, 360
AluI AGCT 2 cut(s) 122, 360
AlwI GGATC 1 cut(s) 85
AoxI GGCC 1 cut(s) 299
ApeKI GCWGC 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 19
AsuNHI GCTAGC 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 2
BbvI GCAGC 1 cut(s) 95
BccI CCATC 1 cut(s) 68
BclI TGATCA 1 cut(s) 282
BfaI CTAG 2 cut(s) 123, 303
BfmI CTRYAG 1 cut(s) 81
BglII AGATCT 1 cut(s) 232
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
BmcAI AGTACT 1 cut(s) 136
BmtI GCTAGC 1 cut(s) 126
BpiI GAAGAC 1 cut(s) 2
BpmI CTGGAG 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 34, 320
Bse1I ACTGG 1 cut(s) 140
BseDI CCNNGG 2 cut(s) 34, 320
BseNI ACTGG 1 cut(s) 140
BseXI GCAGC 1 cut(s) 95
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 1 cut(s) 301
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 1 cut(s) 301
Bsp143I GATC 4 cut(s) 77, 200, 232, 282
Bsp19I CCATGG 1 cut(s) 34
Bsp68I TCGCGA 1 cut(s) 205
BspACI CCGC 2 cut(s) 64, 350
BspANI GGCC 1 cut(s) 301
BspFNI CGCG 1 cut(s) 205
BspMAI CTGCAG 1 cut(s) 85
BspOI GCTAGC 1 cut(s) 126
BspPI GGATC 1 cut(s) 85
BsrI ACTGG 1 cut(s) 140
BssECI CCNNGG 2 cut(s) 34, 320
BssMI GATC 4 cut(s) 77, 200, 232, 282
BssT1I CCWWGG 2 cut(s) 34, 320
Bst4CI ACNGT 2 cut(s) 71, 179
BstC8I GCNNGC 1 cut(s) 124
BstDSI CCRYGG 1 cut(s) 34
BstFNI CGCG 1 cut(s) 205
BstKTI GATC 4 cut(s) 80, 203, 235, 285
BstMBI GATC 4 cut(s) 77, 200, 232, 282
BstMWI GCNNNNNNNGC 3 cut(s) 128, 251, 331
BstNSI RCATGY 1 cut(s) 350
BstSFI CTRYAG 1 cut(s) 81
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 1 cut(s) 95
BstV2I GAAGAC 1 cut(s) 2
BstX2I RGATCY 2 cut(s) 77, 232
BstYI RGATCY 2 cut(s) 77, 232
BsuRI GGCC 1 cut(s) 301
BtgI CCRYGG 1 cut(s) 34
BtsIMutI CAGTG 1 cut(s) 76
BtuMI TCGCGA 1 cut(s) 205
Cac8I GCNNGC 1 cut(s) 124
Csp6I GTAC 2 cut(s) 135, 330
CviAII CATG 3 cut(s) 35, 127, 347
CviJI RGCY 8 cut(s) 122, 147, 227, 245, 289, 301, 325, 360
CviKI_1 RGCY 8 cut(s) 122, 147, 227, 245, 289, 301, 325, 360
CviQI GTAC 2 cut(s) 135, 330
DpnI GATC 4 cut(s) 79, 202, 234, 284
DpnII GATC 4 cut(s) 77, 200, 232, 282
Eco130I CCWWGG 2 cut(s) 34, 320
Eco147I AGGCCT 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 249
EcoT14I CCWWGG 2 cut(s) 34, 320
ErhI CCWWGG 2 cut(s) 34, 320
FaeI CATG 3 cut(s) 38, 130, 350
FaiI YATR 6 cut(s) 36, 128, 189, 296, 348, 373
FatI CATG 3 cut(s) 34, 126, 346
FauI CCCGC 1 cut(s) 343
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 2 cut(s) 123, 303
GluI GCNGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 123
HaeIII GGCC 1 cut(s) 301
Hin1II CATG 3 cut(s) 38, 130, 350
HphI GGTGA 1 cut(s) 19
Hpy188I TCNGA 1 cut(s) 270
Hpy188III TCNNGA 4 cut(s) 164, 173, 204, 259
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 2 cut(s) 71, 179
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 2 cut(s) 83, 155
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 251, 331
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 3 cut(s) 38, 130, 350
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 4 cut(s) 77, 200, 232, 282
LmnI GCTCC 1 cut(s) 38
LpnPI CCDG 2 cut(s) 149, 153
Lsp1109I GCAGC 1 cut(s) 95
MaeI CTAG 2 cut(s) 123, 303
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 2 cut(s) 25, 182
MalI GATC 4 cut(s) 79, 202, 234, 284
MboI GATC 4 cut(s) 77, 200, 232, 282
MboII GAAGA 7 cut(s) 5, 56, 93, 106, 203, 242, 289
MflI RGATCY 2 cut(s) 77, 232
MluCI AATT 1 cut(s) 105
MnlI CCTC 4 cut(s) 27, 50, 235, 276
Mva1269I GAATGC 1 cut(s) 321
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 3 cut(s) 128, 251, 331
NcoI CCATGG 1 cut(s) 34
NdeII GATC 4 cut(s) 77, 200, 232, 282
NheI GCTAGC 1 cut(s) 122
NlaIII CATG 3 cut(s) 38, 130, 350
NmuCI GTSAC 1 cut(s) 25
NruI TCGCGA 1 cut(s) 205
NspI RCATGY 1 cut(s) 350
PceI AGGCCT 1 cut(s) 301
PctI GAATGC 1 cut(s) 321
PkrI GCNGC 1 cut(s) 85
PsiI TTATAA 2 cut(s) 296, 373
PstI CTGCAG 1 cut(s) 85
PsuI RGATCY 2 cut(s) 77, 232
RruI TCGCGA 1 cut(s) 205
RsaI GTAC 2 cut(s) 136, 331
RsaNI GTAC 2 cut(s) 135, 330
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 4 cut(s) 77, 200, 232, 282
ScaI AGTACT 1 cut(s) 136
SetI ASST 5 cut(s) 61, 124, 268, 276, 362
SfcI CTRYAG 1 cut(s) 81
Sse9I AATT 1 cut(s) 105
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 2 cut(s) 64, 350
SspMI CTAG 2 cut(s) 123, 303
StuI AGGCCT 1 cut(s) 301
StyI CCWWGG 2 cut(s) 34, 320
TaaI ACNGT 2 cut(s) 71, 179
TaiI ACGT 1 cut(s) 276
TasI AATT 1 cut(s) 105
TatI WGTACW 1 cut(s) 134
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 1 cut(s) 204
TspRI CASTG 1 cut(s) 76
XceI RCATGY 1 cut(s) 350
XspI CTAG 2 cut(s) 123, 303
ZrmI AGTACT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.