FvH4_5g20250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014747
Physical Location & Seq
Reverse (-)
11961218 .. 11965809
4592 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20250.t2

Sequence Viewer

Length: 939 bp
ATGGACTCCGACTCCGACGACTCCTCCTCCAGCACCGACTCCACCACTTCCATTCCCGCAGCCTCCGCCGGAATCCTCATGCTCTCGCTCCCGGACAGTCTAGACGACGACGGAGCCAACCACGACGACGACAACAAGAGCAGCGCCGACCAGGACGTCTTCGAGACGCCGCCGGAGTACGCGCTCGTCGGAACCTCCCAGGAGAAGACTCCCTTGCTCGATCTTGACCAGATCGGCGACGATCGGGATTACGAGGGAGTTGGAGTTATCGGAGACGGAAGCGGCGTCATTGTGGCGGTCGGTGGTTGCGGCGAGGAAGGCGTTGATGCTGTGGATTTGGGAAGGGATACGGATTTTGTGGTTACCGAGGCGGAATCGGTGGAGTTTAAGGTTTCGAAGAGGGATTGGTCTTTGGAAGGGGGCTCGGCCGAGTCTCCGCCGCCGTCGAAGAAATTGAGGGTTTCGGAGGACGATTCGGGGAAATCAGATGAGGAATTGGAGACCGAGCCGCTCAGTCTGAGCAACCTAGGGTCTAATTCTGATCAAACTCTCGAAATTGCAGGGGATGATGCGAAATCAGTTGAGGAAAGCTCAGGAAAGAAGAAGGATGAAAATTCAGGCAGCGAGAGTGAGAATGGAACTGAAGAGATTCAATCAGTTACTGAAGAAACCGGTAGCCCGTCCGGCATGGAGTATTTGTTTGAGGAAGACGGGGAGATTGACTCCAGGGATTCGCAAGTGCTTCGAGTGCTGCCTCTATCCTTCTGCCCACCAGCAGAGTCGAGTAGGATGCAGGCCGAGGAGGGGGACGAGTGGTCTAGACTATTGGAAATGGTGAAACTTGTGAACGATATAACTTGCAAGGAGGACAATGAGGTTGATAACTGTAGCCTTTTGGAGATAGCTAAGCGCCGCGGCGTGACTTTCCCTCGGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

33.37

Weight (kDa)

4.05

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 155
AatII GACGTC 1 cut(s) 159
AccBSI CCGCTC 1 cut(s) 511
AccII CGCG 2 cut(s) 182, 915
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 1 cut(s) 613
AcuI CTGAAG 2 cut(s) 663, 684
AcyI GRCGYC 3 cut(s) 156, 167, 285
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 804
AgeI ACCGGT 1 cut(s) 671
AgsI TTSAA 1 cut(s) 653
AhdI GACNNNNNGTC 1 cut(s) 814
AjnI CCWGG 3 cut(s) 150, 198, 725
AluBI AGCT 2 cut(s) 591, 905
AluI AGCT 2 cut(s) 591, 905
Alw26I GTCTC 4 cut(s) 158, 267, 438, 494
AlwNI CAGNNNCTG 1 cut(s) 662
AoxI GGCC 2 cut(s) 426, 795
ApeKI GCWGC 4 cut(s) 59, 141, 621, 751
ApoI RAATTY 1 cut(s) 613
AsiGI ACCGGT 1 cut(s) 671
Asp700I GAANNNNTTC 1 cut(s) 648
AspA2I CCTAGG 1 cut(s) 526
AspLEI GCGC 3 cut(s) 146, 184, 912
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 847
AsuII TTCGAA 1 cut(s) 395
AvrII CCTAGG 1 cut(s) 526
BanII GRGCYC 1 cut(s) 425
BbsI GAAGAC 3 cut(s) 151, 212, 714
BbvI GCAGC 4 cut(s) 71, 153, 633, 738
BceAI ACGGC 1 cut(s) 427
BcgI CGANNNNNNTGC 4 cut(s) 725, 759, 772, 806
BciT130I CCWGG 3 cut(s) 152, 200, 727
BciVI GTATCC 1 cut(s) 340
BclI TGATCA 1 cut(s) 541
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 4 cut(s) 158, 267, 438, 494
BfaI CTAG 3 cut(s) 101, 527, 819
BfmI CTRYAG 2 cut(s) 886, 935
BfoI RGCGCY 2 cut(s) 147, 913
BfuI GTATCC 1 cut(s) 340
BglI GCCNNNNNGGC 1 cut(s) 684
BlnI CCTAGG 1 cut(s) 526
BlpI GCTNAGC 1 cut(s) 906
Bme1390I CCNGG 4 cut(s) 92, 152, 200, 727
BmeRI GACNNNNNGTC 1 cut(s) 814
BmiI GGNNCC 2 cut(s) 115, 193
BmrFI CCNGG 4 cut(s) 92, 152, 200, 727
BmsI GCATC 3 cut(s) 316, 559, 780
BpiI GAAGAC 3 cut(s) 151, 212, 714
BplI GAGNNNNNCTC 4 cut(s) 575, 607, 707, 739
BpmI CTGGAG 2 cut(s) 13, 709
Bpu10I CCTNAGC 1 cut(s) 592
Bpu1102I GCTNAGC 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 92
BsaHI GRCGYC 3 cut(s) 156, 167, 285
BsaI GGTCTC 1 cut(s) 494
BsaJI CCNNGG 7 cut(s) 198, 366, 526, 726, 798, 913, 929
BsaWI WCCGGW 1 cut(s) 671
BsaXI ACNNNNNCTCC 9 cut(s) 8, 11, 26, 38, 41, 249, 279, 683, 713
Bsc4I CCNNNNNNNGG 1 cut(s) 804
Bse118I RCCGGY 1 cut(s) 671
BseBI CCWGG 3 cut(s) 152, 200, 727
BseDI CCNNGG 7 cut(s) 198, 366, 526, 726, 798, 913, 929
BseGI GGATG 3 cut(s) 571, 613, 795
BseLI CCNNNNNNNGG 1 cut(s) 804
BseMII CTCAG 3 cut(s) 509, 526, 606
BseRI GAGGAG 3 cut(s) 13, 16, 815
BseX3I CGGCCG 1 cut(s) 426
BseXI GCAGC 4 cut(s) 71, 153, 633, 738
Bsh1236I CGCG 2 cut(s) 182, 915
Bsh1285I CGRYCG 3 cut(s) 244, 300, 429
BshFI GGCC 2 cut(s) 428, 797
BshTI ACCGGT 1 cut(s) 671
BsiEI CGRYCG 3 cut(s) 244, 300, 429
BsiSI CCGG 5 cut(s) 69, 92, 173, 672, 684
BslFI GGGAC 1 cut(s) 821
BslI CCNNNNNNNGG 1 cut(s) 804
BsmAI GTCTC 4 cut(s) 158, 267, 438, 494
BsmBI CGTCTC 2 cut(s) 158, 267
BsmFI GGGAC 1 cut(s) 821
BsnI GGCC 2 cut(s) 428, 797
Bso31I GGTCTC 1 cut(s) 494
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 425
Bsp143I GATC 4 cut(s) 220, 231, 241, 541
Bsp1720I GCTNAGC 1 cut(s) 906
BspANI GGCC 2 cut(s) 428, 797
BspCNI CTCAG 3 cut(s) 510, 525, 605
BspFNI CGCG 2 cut(s) 182, 915
BspLI GGNNCC 2 cut(s) 115, 193
BspT104I TTCGAA 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 494
BsrBI CCGCTC 1 cut(s) 511
BsrFI RCCGGY 1 cut(s) 671
BssAI RCCGGY 1 cut(s) 671
BssECI CCNNGG 7 cut(s) 198, 366, 526, 726, 798, 913, 929
BssMI GATC 4 cut(s) 220, 231, 241, 541
BssNI GRCGYC 3 cut(s) 156, 167, 285
BssT1I CCWWGG 1 cut(s) 526
Bst2UI CCWGG 3 cut(s) 152, 200, 727
Bst4CI ACNGT 2 cut(s) 98, 887
Bst6I CTCTTC 2 cut(s) 392, 639
BstACI GRCGYC 3 cut(s) 156, 167, 285
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 795
BstDEI CTNAG 4 cut(s) 512, 518, 592, 906
BstDSI CCRYGG 1 cut(s) 913
BstEII GGTNACC 1 cut(s) 361
BstF5I GGATG 3 cut(s) 571, 613, 795
BstFNI CGCG 2 cut(s) 182, 915
BstH2I RGCGCY 2 cut(s) 147, 913
BstHHI GCGC 3 cut(s) 146, 184, 912
BstKTI GATC 4 cut(s) 223, 234, 244, 544
BstMAI GTCTC 4 cut(s) 158, 267, 438, 494
BstMBI GATC 4 cut(s) 220, 231, 241, 541
BstMCI CGRYCG 3 cut(s) 244, 300, 429
BstMWI GCNNNNNNNGC 4 cut(s) 65, 318, 684, 748
BstNI CCWGG 3 cut(s) 152, 200, 727
BstPI GGTNACC 1 cut(s) 361
BstSCI CCNGG 4 cut(s) 90, 150, 198, 725
BstSFI CTRYAG 2 cut(s) 886, 935
BstUI CGCG 2 cut(s) 182, 915
BstV1I GCAGC 4 cut(s) 71, 153, 633, 738
BstV2I GAAGAC 3 cut(s) 151, 212, 714
BstZI CGGCCG 1 cut(s) 426
BsuI GTATCC 1 cut(s) 340
BsuRI GGCC 2 cut(s) 428, 797
BtgI CCRYGG 1 cut(s) 913
BtsCI GGATG 3 cut(s) 571, 613, 795
Cac8I GCNNGC 1 cut(s) 795
CaiI CAGNNNCTG 1 cut(s) 662
CfoI GCGC 3 cut(s) 146, 184, 912
Cfr10I RCCGGY 1 cut(s) 671
Cfr42I CCGCGG 1 cut(s) 916
CseI GACGC 2 cut(s) 175, 274
Csp6I GTAC 1 cut(s) 178
CspAI ACCGGT 1 cut(s) 671
CviAII CATG 2 cut(s) 79, 688
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 4 cut(s) 512, 518, 592, 906
DpnI GATC 4 cut(s) 222, 233, 243, 543
DpnII GATC 4 cut(s) 220, 231, 241, 541
DrdI GACNNNNNNGTC 1 cut(s) 155
DriI GACNNNNNGTC 1 cut(s) 814
DseDI GACNNNNNNGTC 1 cut(s) 155
EaeI YGGCCR 1 cut(s) 426
EagI CGGCCG 1 cut(s) 426
Eam1104I CTCTTC 2 cut(s) 392, 639
Eam1105I GACNNNNNGTC 1 cut(s) 814
EarI CTCTTC 2 cut(s) 392, 639
EciI GGCGGA 3 cut(s) 55, 386, 426
EclXI CGGCCG 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 526
Eco24I GRGCYC 1 cut(s) 425
Eco31I GGTCTC 1 cut(s) 494
Eco52I CGGCCG 1 cut(s) 426
Eco57I CTGAAG 2 cut(s) 663, 684
Eco91I GGTNACC 1 cut(s) 361
EcoO65I GGTNACC 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 150, 198, 725
EcoT14I CCWWGG 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 425
ErhI CCWWGG 1 cut(s) 526
Esp3I CGTCTC 2 cut(s) 158, 267
FaeI CATG 2 cut(s) 82, 691
FaiI YATR 3 cut(s) 80, 689, 854
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 1 cut(s) 821
FatI CATG 2 cut(s) 78, 687
FauI CCCGC 1 cut(s) 64
FbaI TGATCA 1 cut(s) 541
FokI GGATG 3 cut(s) 578, 620, 802
FriOI GRGCYC 1 cut(s) 425
FspBI CTAG 3 cut(s) 101, 527, 819
GlaI GCGC 3 cut(s) 145, 183, 911
GsuI CTGGAG 2 cut(s) 13, 709
HaeII RGCGCY 2 cut(s) 147, 913
HaeIII GGCC 2 cut(s) 428, 797
HapII CCGG 5 cut(s) 69, 92, 173, 672, 684
HgaI GACGC 2 cut(s) 175, 274
HhaI GCGC 3 cut(s) 146, 184, 912
Hin1I GRCGYC 3 cut(s) 156, 167, 285
Hin1II CATG 2 cut(s) 82, 691
Hin6I GCGC 3 cut(s) 144, 182, 910
HinP1I GCGC 3 cut(s) 144, 182, 910
HpaII CCGG 5 cut(s) 69, 92, 173, 672, 684
HphI GGTGA 1 cut(s) 847
Hpy166II GTNNAC 1 cut(s) 847
Hpy188I TCNGA 8 cut(s) 10, 16, 191, 272, 466, 487, 519, 541
Hpy188III TCNNGA 7 cut(s) 101, 163, 224, 245, 551, 594, 819
Hpy8I GTNNAC 1 cut(s) 847
Hpy99I CGWCG 8 cut(s) 20, 110, 113, 128, 131, 191, 242, 448
HpyAV CCTTC 5 cut(s) 311, 336, 410, 598, 772
HpyCH4III ACNGT 2 cut(s) 98, 887
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 3 cut(s) 560, 793, 861
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 318, 684, 748
HpyF3I CTNAG 4 cut(s) 512, 518, 592, 906
HpySE526I ACGT 1 cut(s) 156
Hsp92I GRCGYC 3 cut(s) 156, 167, 285
Hsp92II CATG 2 cut(s) 82, 691
HspAI GCGC 3 cut(s) 144, 182, 910
Ksp22I TGATCA 1 cut(s) 541
KspI CCGCGG 1 cut(s) 916
Kzo9I GATC 4 cut(s) 220, 231, 241, 541
LmnI GCTCC 2 cut(s) 93, 113
Lsp1109I GCAGC 4 cut(s) 71, 153, 633, 738
LweI GCATC 3 cut(s) 316, 559, 780
MaeI CTAG 3 cut(s) 101, 527, 819
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 3 cut(s) 361, 658, 919
MalI GATC 4 cut(s) 222, 233, 243, 543
MbiI CCGCTC 1 cut(s) 511
MboI GATC 4 cut(s) 220, 231, 241, 541
MboII GAAGA 8 cut(s) 151, 217, 409, 460, 613, 656, 677, 719
MhlI GDGCHC 1 cut(s) 425
MluCI AATT 5 cut(s) 452, 494, 535, 555, 613
MlyI GAGTC 7 cut(s) 5, 14, 32, 202, 440, 716, 788
MmeI TCCRAC 4 cut(s) 33, 39, 169, 241
MroXI GAANNNNTTC 1 cut(s) 648
MseI TTAA 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 915
MspI CCGG 5 cut(s) 69, 92, 173, 672, 684
MspR9I CCNGG 4 cut(s) 92, 152, 200, 727
MvaI CCWGG 3 cut(s) 152, 200, 727
MvnI CGCG 2 cut(s) 182, 915
MwoI GCNNNNNNNGC 4 cut(s) 65, 318, 684, 748
NciI CCSGG 1 cut(s) 92
NdeII GATC 4 cut(s) 220, 231, 241, 541
NlaIII CATG 2 cut(s) 82, 691
NlaIV GGNNCC 2 cut(s) 115, 193
NmeAIII GCCGAG 4 cut(s) 404, 454, 823, 910
NmuCI GTSAC 1 cut(s) 919
NspV TTCGAA 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 5 cut(s) 72, 374, 473, 649, 731
PfoI TCCNGGA 1 cut(s) 90
PinAI ACCGGT 1 cut(s) 671
Ple19I CGATCG 1 cut(s) 244
PleI GAGTC 7 cut(s) 5, 14, 32, 202, 439, 716, 787
PpsI GAGTC 7 cut(s) 5, 14, 32, 202, 439, 716, 787
Psp6I CCWGG 3 cut(s) 150, 198, 725
PspEI GGTNACC 1 cut(s) 361
PspGI CCWGG 3 cut(s) 150, 198, 725
PspN4I GGNNCC 2 cut(s) 115, 193
PstNI CAGNNNCTG 1 cut(s) 662
PvuI CGATCG 1 cut(s) 244
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SacII CCGCGG 1 cut(s) 916
SaqAI TTAA 1 cut(s) 387
Sau3AI GATC 4 cut(s) 220, 231, 241, 541
SchI GAGTC 7 cut(s) 5, 14, 32, 202, 440, 716, 788
ScrFI CCNGG 4 cut(s) 92, 152, 200, 727
SduI GDGCHC 1 cut(s) 425
SetI ASST 7 cut(s) 159, 197, 393, 528, 593, 879, 907
SfaNI GCATC 3 cut(s) 316, 559, 780
SfcI CTRYAG 2 cut(s) 886, 935
Sfr303I CCGCGG 1 cut(s) 916
SfuI TTCGAA 1 cut(s) 395
SgrBI CCGCGG 1 cut(s) 916
Sse9I AATT 5 cut(s) 452, 494, 535, 555, 613
SspMI CTAG 3 cut(s) 101, 527, 819
StyD4I CCNGG 4 cut(s) 90, 150, 198, 725
StyI CCWWGG 1 cut(s) 526
TaaI ACNGT 2 cut(s) 98, 887
TaiI ACGT 1 cut(s) 159
TaqI TCGA 7 cut(s) 162, 219, 395, 446, 552, 745, 782
TaqII GACCGA 1 cut(s) 518
TasI AATT 5 cut(s) 452, 494, 535, 555, 613
TauI GCSGC 7 cut(s) 172, 285, 312, 442, 511, 915, 918
TfiI GAWTC 5 cut(s) 72, 374, 473, 649, 731
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TseFI GTSAC 1 cut(s) 919
TseI GCWGC 4 cut(s) 59, 141, 621, 751
Tsp45I GTSAC 1 cut(s) 919
TspDTI ATGAA 1 cut(s) 624
TspGWI ACGGA 3 cut(s) 126, 291, 365
XapI RAATTY 1 cut(s) 613
XbaI TCTAGA 2 cut(s) 100, 818
XmaJI CCTAGG 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 648
XspI CTAG 3 cut(s) 101, 527, 819
ZraI GACGTC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.