Rh7AG220400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014747
Physical Location & Seq
Reverse (-)
20490358 .. 20491284
927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG220400.1

Sequence Viewer

Length: 927 bp
ATGGACGATGACTACTCCAGTACCGACTCATCTTCCGATTCCAGTACTACTATTCCGGCAGCCACCGCCGGAATCGTCATGCTCTCTCTCCCGGACAGTTTCGATGAGGATGCAGCCGAGGACAAGAGCAGCGCCGACCAGGACGTTTTCGAGACCCCGCCGGAGTCCTCGCTCGTCGGAACCTCCCAGGAGAAGACTCCCTTGCTCGATCTTGATCAGATCGGCGATGACGATGATCGTGATTTCGAGGGAGTTGGAGATATAGGAGATGGGAGCGGCGTCATCGTGGCCGTCGGTGGTTGCGGCGAGGAAGGCGACGGTGCTGTGGATTTGGGGCGGGATGCTGATCTGGTGACTTCGGAGGCGGAATCGGTGGAATTTAGGGTTTCGAAGAGGGATTTGTCGATGGAAGGGAGTTCGGAGGAGTCTTCGCCGCCGTCGAAGAAATTGAGGGTTTCGGAGCAGGAATCGGGGAAATCAGAGGAGGAATTGGAGACCGAGCGGCTCAGTCTGAGCAACCTCGGCTCTGATTCCGATCAAACTCTCGAAATTGCAGGGGATGATGCCAAATCAGTTGAGGAAAGCTCAGGAAAGAAGAATGCTGAGTATTCAGACAGCGAGAGTGAGAATGCTACTGAAGAGACTCAACCGGTTACTGAAGAAAGCGGAAGCCCATCCGGCGTGGAGTATTCCTATCAGGAAGGCAGTGAAATTGATTCCGGAGTGCTTCGAGTGCTGCCTCTATCCTTCTGCCCACCAGCAGACTCGAGTGAGAGGCATGCCGAGGAGGGGCATAGCTGGTCTAGTCTGTTGGAGACGTTGAAACGTGTGAGAGAAATAACTCGTCAAGACTGTAAAAATAAGGTTGATAAATGTAGCTTTTTGGAGATAGCAAAGCGTCGCGGCATGACTTTCCCTCAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

32.98

Weight (kDa)

4.07

Isoelectric Point (pI)

62.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 276, 502
AccII CGCG 1 cut(s) 903
AccIII TCCGGA 1 cut(s) 719
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 2 cut(s) 657, 678
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 2 cut(s) 22, 46
AfiI CCNNNNNNNGG 1 cut(s) 789
AflIII ACRYGT 1 cut(s) 826
AgeI ACCGGT 1 cut(s) 649
AgsI TTSAA 1 cut(s) 823
AjnI CCWGG 2 cut(s) 138, 186
AluBI AGCT 4 cut(s) 585, 798, 879, 922
AluI AGCT 4 cut(s) 585, 798, 879, 922
Alw26I GTCTC 4 cut(s) 146, 488, 635, 809
Ama87I CYCGRG 1 cut(s) 766
Aor13HI TCCGGA 1 cut(s) 719
AoxI GGCC 1 cut(s) 288
ApeKI GCWGC 4 cut(s) 59, 113, 129, 736
ApoI RAATTY 1 cut(s) 377
AsiGI ACCGGT 1 cut(s) 649
AspLEI GCGC 1 cut(s) 134
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 364
AsuII TTCGAA 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 766
BaeI ACNNNNGTAYC 1 cut(s) 37
BbsI GAAGAC 2 cut(s) 200, 420
BbvCI CCTCAGC 1 cut(s) 918
BbvI GCAGC 4 cut(s) 71, 125, 141, 723
BccI CCATC 3 cut(s) 263, 400, 682
BceAI ACGGC 2 cut(s) 275, 421
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 2 cut(s) 140, 188
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 4 cut(s) 146, 488, 635, 809
BfaI CTAG 1 cut(s) 804
BfmI CTRYAG 1 cut(s) 923
BfoI RGCGCY 1 cut(s) 135
BglI GCCNNNNNGGC 1 cut(s) 678
BisI GCNGC 9 cut(s) 60, 114, 130, 277, 304, 434, 503, 737, 904
BlsI GCNGC 9 cut(s) 61, 115, 131, 278, 305, 435, 504, 738, 905
BmcAI AGTACT 1 cut(s) 46
Bme1390I CCNGG 3 cut(s) 92, 140, 188
BmeT110I CYCGRG 1 cut(s) 766
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 3 cut(s) 92, 140, 188
BmsI GCATC 3 cut(s) 100, 331, 553
BpiI GAAGAC 2 cut(s) 200, 420
BplI GAGNNNNNCTC 2 cut(s) 569, 601
Bpu10I CCTNAGC 2 cut(s) 586, 918
Bpu14I TTCGAA 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 92
BsaBI GATNNNNATC 3 cut(s) 213, 345, 534
BsaHI GRCGYC 1 cut(s) 279
BsaI GGTCTC 2 cut(s) 146, 488
BsaJI CCNNGG 4 cut(s) 117, 186, 520, 783
BsaWI WCCGGW 2 cut(s) 649, 719
Bsc4I CCNNNNNNNGG 1 cut(s) 789
Bse118I RCCGGY 1 cut(s) 649
Bse1I ACTGG 2 cut(s) 18, 42
Bse8I GATNNNNATC 3 cut(s) 213, 345, 534
BseAI TCCGGA 1 cut(s) 719
BseBI CCWGG 2 cut(s) 140, 188
BseDI CCNNGG 4 cut(s) 117, 186, 520, 783
BseGI GGATG 4 cut(s) 115, 346, 565, 674
BseJI GATNNNNATC 3 cut(s) 213, 345, 534
BseLI CCNNNNNNNGG 1 cut(s) 789
BseMII CTCAG 4 cut(s) 503, 520, 594, 600
BseNI ACTGG 2 cut(s) 18, 42
BseRI GAGGAG 3 cut(s) 437, 497, 800
BseXI GCAGC 4 cut(s) 71, 125, 141, 723
Bsh1236I CGCG 1 cut(s) 903
BshFI GGCC 1 cut(s) 290
BshTI ACCGGT 1 cut(s) 649
BsiHKCI CYCGRG 1 cut(s) 766
BsiSI CCGG 7 cut(s) 56, 69, 92, 161, 650, 678, 720
BslI CCNNNNNNNGG 1 cut(s) 789
BsmAI GTCTC 4 cut(s) 146, 488, 635, 809
BsmBI CGTCTC 1 cut(s) 809
BsmI GAATGC 2 cut(s) 604, 634
BsnI GGCC 1 cut(s) 290
Bso31I GGTCTC 2 cut(s) 146, 488
BsoBI CYCGRG 1 cut(s) 766
Bsp119I TTCGAA 1 cut(s) 389
Bsp13I TCCGGA 1 cut(s) 719
Bsp143I GATC 6 cut(s) 208, 214, 219, 235, 346, 535
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 4 cut(s) 504, 519, 595, 599
BspEI TCCGGA 1 cut(s) 719
BspFNI CGCG 1 cut(s) 903
BspLI GGNNCC 1 cut(s) 181
BspT104I TTCGAA 1 cut(s) 389
BspTNI GGTCTC 2 cut(s) 146, 488
BsrBI CCGCTC 2 cut(s) 276, 502
BsrFI RCCGGY 1 cut(s) 649
BsrI ACTGG 2 cut(s) 18, 42
BssAI RCCGGY 1 cut(s) 649
BssECI CCNNGG 4 cut(s) 117, 186, 520, 783
BssMI GATC 6 cut(s) 208, 214, 219, 235, 346, 535
BssNI GRCGYC 1 cut(s) 279
Bst2UI CCWGG 2 cut(s) 140, 188
Bst4CI ACNGT 3 cut(s) 98, 320, 854
Bst6I CTCTTC 2 cut(s) 386, 633
BstACI GRCGYC 1 cut(s) 279
BstBI TTCGAA 1 cut(s) 389
BstC8I GCNNGC 1 cut(s) 780
BstDEI CTNAG 5 cut(s) 506, 512, 586, 603, 918
BstF5I GGATG 4 cut(s) 115, 346, 565, 674
BstFNI CGCG 1 cut(s) 903
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 6 cut(s) 211, 217, 222, 238, 349, 538
BstMAI GTCTC 4 cut(s) 146, 488, 635, 809
BstMBI GATC 6 cut(s) 208, 214, 219, 235, 346, 535
BstMWI GCNNNNNNNGC 5 cut(s) 65, 312, 522, 678, 733
BstNI CCWGG 2 cut(s) 140, 188
BstNSI RCATGY 1 cut(s) 782
BstSCI CCNGG 3 cut(s) 90, 138, 186
BstSFI CTRYAG 1 cut(s) 923
BstUI CGCG 1 cut(s) 903
BstV1I GCAGC 4 cut(s) 71, 125, 141, 723
BstV2I GAAGAC 2 cut(s) 200, 420
BsuRI GGCC 1 cut(s) 290
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 4 cut(s) 115, 346, 565, 674
BtsI GCAGTG 1 cut(s) 712
BtsIMutI CAGTG 1 cut(s) 712
Cac8I GCNNGC 1 cut(s) 780
CfoI GCGC 1 cut(s) 134
Cfr10I RCCGGY 1 cut(s) 649
CseI GACGC 2 cut(s) 268, 887
Csp6I GTAC 2 cut(s) 21, 45
CspAI ACCGGT 1 cut(s) 649
CviAII CATG 3 cut(s) 79, 779, 907
CviQI GTAC 2 cut(s) 21, 45
DdeI CTNAG 5 cut(s) 506, 512, 586, 603, 918
DpnI GATC 6 cut(s) 210, 216, 221, 237, 348, 537
DpnII GATC 6 cut(s) 208, 214, 219, 235, 346, 535
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 2 cut(s) 386, 633
EarI CTCTTC 2 cut(s) 386, 633
EciI GGCGGA 1 cut(s) 380
Eco31I GGTCTC 2 cut(s) 146, 488
Eco57I CTGAAG 2 cut(s) 657, 678
Eco88I CYCGRG 1 cut(s) 766
EcoRII CCWGG 2 cut(s) 138, 186
Esp3I CGTCTC 1 cut(s) 809
FaeI CATG 3 cut(s) 82, 782, 910
FaiI YATR 5 cut(s) 80, 263, 780, 795, 908
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FatI CATG 3 cut(s) 78, 778, 906
FauI CCCGC 2 cut(s) 165, 330
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 9 cut(s) 60, 114, 130, 277, 304, 434, 503, 737, 904
FokI GGATG 4 cut(s) 122, 353, 572, 661
Fsp4HI GCNGC 9 cut(s) 60, 114, 130, 277, 304, 434, 503, 737, 904
FspBI CTAG 1 cut(s) 804
GlaI GCGC 1 cut(s) 133
GluI GCNGC 9 cut(s) 60, 114, 130, 277, 304, 434, 503, 737, 904
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 1 cut(s) 290
HapII CCGG 7 cut(s) 56, 69, 92, 161, 650, 678, 720
HgaI GACGC 2 cut(s) 268, 887
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 3 cut(s) 82, 782, 910
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HpaII CCGG 7 cut(s) 56, 69, 92, 161, 650, 678, 720
HphI GGTGA 1 cut(s) 364
Hpy188III TCNNGA 8 cut(s) 151, 212, 239, 545, 588, 698, 720, 848
Hpy99I CGWCG 5 cut(s) 179, 296, 320, 442, 903
HpyAV CCTTC 4 cut(s) 305, 404, 695, 757
HpyCH4III ACNGT 3 cut(s) 98, 320, 854
HpyCH4IV ACGT 3 cut(s) 144, 818, 826
HpyCH4V TGCA 2 cut(s) 113, 554
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 312, 522, 678, 733
HpyF3I CTNAG 5 cut(s) 506, 512, 586, 603, 918
HpySE526I ACGT 3 cut(s) 144, 818, 826
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 3 cut(s) 82, 782, 910
HspAI GCGC 1 cut(s) 132
Kpn2I TCCGGA 1 cut(s) 719
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 6 cut(s) 208, 214, 219, 235, 346, 535
LmnI GCTCC 2 cut(s) 273, 460
Lsp1109I GCAGC 4 cut(s) 71, 125, 141, 723
LweI GCATC 3 cut(s) 100, 331, 553
MaeI CTAG 1 cut(s) 804
MaeII ACGT 3 cut(s) 144, 818, 826
MaeIII GTNAC 2 cut(s) 352, 652
MalI GATC 6 cut(s) 210, 216, 221, 237, 348, 537
MbiI CCGCTC 2 cut(s) 276, 502
MboI GATC 6 cut(s) 208, 214, 219, 235, 346, 535
MboII GAAGA 8 cut(s) 24, 205, 403, 420, 454, 607, 650, 671
MluCI AATT 5 cut(s) 377, 446, 488, 549, 711
MlyI GAGTC 6 cut(s) 20, 173, 190, 434, 637, 758
MmeI TCCRAC 3 cut(s) 157, 235, 792
MroI TCCGGA 1 cut(s) 719
MspA1I CMGCKG 1 cut(s) 922
MspI CCGG 7 cut(s) 56, 69, 92, 161, 650, 678, 720
MspR9I CCNGG 3 cut(s) 92, 140, 188
Mva1269I GAATGC 2 cut(s) 604, 634
MvaI CCWGG 2 cut(s) 140, 188
MvnI CGCG 1 cut(s) 903
MwoI GCNNNNNNNGC 5 cut(s) 65, 312, 522, 678, 733
NciI CCSGG 1 cut(s) 92
NdeII GATC 6 cut(s) 208, 214, 219, 235, 346, 535
NlaIII CATG 3 cut(s) 82, 782, 910
NlaIV GGNNCC 1 cut(s) 181
NmeAIII GCCGAG 3 cut(s) 142, 501, 808
NmuCI GTSAC 1 cut(s) 352
NspI RCATGY 1 cut(s) 782
NspV TTCGAA 1 cut(s) 389
PaeI GCATGC 1 cut(s) 782
PaeR7I CTCGAG 1 cut(s) 766
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 437
PctI GAATGC 2 cut(s) 604, 634
PfeI GAWTC 6 cut(s) 38, 72, 368, 467, 530, 716
PfoI TCCNGGA 1 cut(s) 90
PinAI ACCGGT 1 cut(s) 649
PkrI GCNGC 9 cut(s) 61, 115, 131, 278, 305, 435, 504, 738, 905
PleI GAGTC 6 cut(s) 20, 172, 190, 433, 637, 758
PpsI GAGTC 6 cut(s) 20, 172, 190, 433, 637, 758
Psp6I CCWGG 2 cut(s) 138, 186
PspGI CCWGG 2 cut(s) 138, 186
PspN4I GGNNCC 1 cut(s) 181
PspXI VCTCGAGB 1 cut(s) 766
PvuII CAGCTG 1 cut(s) 922
RsaI GTAC 2 cut(s) 22, 46
RsaNI GTAC 2 cut(s) 21, 45
SatI GCNGC 9 cut(s) 60, 114, 130, 277, 304, 434, 503, 737, 904
Sau3AI GATC 6 cut(s) 208, 214, 219, 235, 346, 535
ScaI AGTACT 1 cut(s) 46
SchI GAGTC 6 cut(s) 20, 173, 190, 434, 637, 758
ScrFI CCNGG 3 cut(s) 92, 140, 188
SfaNI GCATC 3 cut(s) 100, 331, 553
SfcI CTRYAG 1 cut(s) 923
Sfr274I CTCGAG 1 cut(s) 766
SfuI TTCGAA 1 cut(s) 389
SlaI CTCGAG 1 cut(s) 766
SmlI CTYRAG 1 cut(s) 766
SmoI CTYRAG 1 cut(s) 766
SphI GCATGC 1 cut(s) 782
Sse9I AATT 5 cut(s) 377, 446, 488, 549, 711
SspMI CTAG 1 cut(s) 804
StyD4I CCNGG 3 cut(s) 90, 138, 186
TaaI ACNGT 3 cut(s) 98, 320, 854
TaiI ACGT 3 cut(s) 147, 821, 829
TaqII GACCGA 1 cut(s) 512
TasI AATT 5 cut(s) 377, 446, 488, 549, 711
TatI WGTACW 1 cut(s) 44
TauI GCSGC 5 cut(s) 279, 306, 436, 505, 906
TfiI GAWTC 6 cut(s) 38, 72, 368, 467, 530, 716
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 4 cut(s) 59, 113, 129, 736
Tsp45I GTSAC 1 cut(s) 352
TspRI CASTG 1 cut(s) 712
XapI RAATTY 1 cut(s) 377
XceI RCATGY 1 cut(s) 782
XhoI CTCGAG 1 cut(s) 766
XspI CTAG 1 cut(s) 804
ZrmI AGTACT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.