Rmu_sc0009317.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009317.1
OG0014747
Physical Location & Seq
Reverse (-)
1 .. 1306
1306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009317.1_g000001.1.cds

Sequence Viewer

Length: 991 bp
atggacgacgccgatgacgactactctagtaccgactcatcttccgattccagtactactattccggcaacctccgccggaatcgtcatgctctctctcccggacagtttcgatgaggatgcagccgaggacaagagcagcgccgaccaggacgttttcgagaccccgccggagtcctcgcttgtcggaacctcccaggagaagactcccttgctcgatcttgatcagatcggcgatgacgacgacgacgacgatcgtgatttcgagggagttggagatataggagatgggagcggcgtcatcgtggccgtcggtggttgcggcgaggaaggcgacggtgctgtggatttggggcgcgatgctgatctggtgacttcggaggcggaatcggtggaatttagggtttcgaagagggatttgtcgatggaagggagttcggaggagtcttcgccgccgtcgaagaaattgagggtttcggagcaggaatcggggaaatcagaggaggaattggagaccgagcggctaagtctgagcaacctcggctctgattccgatcaaactctcgaaattgcaggggatgatgccaaatcagttgaggaaagctcaggaaagaagaatgctgagtattcagacagcgagagagagagtgctactgaagagactcaaccggttactgaagaaagcggtagcccatccggcgtggagtattcctatcaggaaggcagtgaaattgattccggagtgcttcgagtgctgcctctatccgtctgccccccagcagactcgagtgaggggcatagctggtctagtctgttggagatgttgaaacgtagtggcggctggaagaagggttttgagtcttctgaattggacgaaaccgtattcacaagaaacgaagaagatgagggaaggatttcgggttggaatgctggtttgctaatgctgggttttgaggacagttttgaagagcagcagcgactgaaaatcccag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

330

Amino Acids

35.29

Weight (kDa)

4.05

Isoelectric Point (pI)

59.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 294, 520
AccII CGCG 1 cut(s) 357
AccIII TCCGGA 1 cut(s) 737
AciI CCGC 9 cut(s) 75, 167, 294, 321, 383, 452, 520, 684, 837
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 1 cut(s) 395
AcuI CTGAAG 2 cut(s) 675, 696
AcyI GRCGYC 2 cut(s) 9, 297
AfaI GTAC 2 cut(s) 31, 55
AgeI ACCGGT 1 cut(s) 667
AgsI TTSAA 2 cut(s) 826, 965
AjnI CCWGG 2 cut(s) 147, 195
AluBI AGCT 2 cut(s) 603, 801
AluI AGCT 2 cut(s) 603, 801
Alw26I GTCTC 3 cut(s) 155, 506, 653
AlwNI CAGNNNCTG 1 cut(s) 979
Ama87I CYCGRG 1 cut(s) 784
Aor13HI TCCGGA 1 cut(s) 737
AoxI GGCC 1 cut(s) 306
ApeKI GCWGC 5 cut(s) 122, 138, 754, 970, 973
ApoI RAATTY 1 cut(s) 395
AsiGI ACCGGT 1 cut(s) 667
Asp700I GAANNNNTTC 1 cut(s) 913
AspLEI GCGC 2 cut(s) 143, 357
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 382
AsuII TTCGAA 1 cut(s) 407
AvaI CYCGRG 1 cut(s) 784
BaeI ACNNNNGTAYC 2 cut(s) 13, 46
BbsI GAAGAC 3 cut(s) 209, 438, 852
BbvI GCAGC 5 cut(s) 134, 150, 741, 982, 985
BccI CCATC 3 cut(s) 281, 418, 700
BceAI ACGGC 2 cut(s) 293, 439
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 2 cut(s) 149, 197
BclI TGATCA 1 cut(s) 223
BcnI CCSGG 1 cut(s) 101
BcoDI GTCTC 3 cut(s) 155, 506, 653
BfaI CTAG 2 cut(s) 27, 807
BfoI RGCGCY 1 cut(s) 144
BglI GCCNNNNNGGC 1 cut(s) 696
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 3 cut(s) 101, 149, 197
BmeT110I CYCGRG 1 cut(s) 784
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 3 cut(s) 101, 149, 197
BmsI GCATC 3 cut(s) 109, 349, 571
BpiI GAAGAC 3 cut(s) 209, 438, 852
BplI GAGNNNNNCTC 2 cut(s) 587, 619
Bpu10I CCTNAGC 1 cut(s) 604
Bpu14I TTCGAA 1 cut(s) 407
BpuMI CCSGG 1 cut(s) 101
BsaBI GATNNNNATC 3 cut(s) 222, 363, 552
BsaHI GRCGYC 2 cut(s) 9, 297
BsaI GGTCTC 2 cut(s) 155, 506
BsaJI CCNNGG 3 cut(s) 126, 195, 538
BsaWI WCCGGW 2 cut(s) 667, 737
Bse118I RCCGGY 1 cut(s) 667
Bse1I ACTGG 1 cut(s) 51
Bse8I GATNNNNATC 3 cut(s) 222, 363, 552
BseAI TCCGGA 1 cut(s) 737
BseBI CCWGG 2 cut(s) 149, 197
BseDI CCNNGG 3 cut(s) 126, 195, 538
BseGI GGATG 3 cut(s) 124, 583, 692
BseJI GATNNNNATC 3 cut(s) 222, 363, 552
BseMII CTCAG 3 cut(s) 521, 612, 618
BseNI ACTGG 1 cut(s) 51
BseRI GAGGAG 2 cut(s) 455, 515
BseXI GCAGC 5 cut(s) 134, 150, 741, 982, 985
BseYI CCCAGC 2 cut(s) 775, 943
Bsh1236I CGCG 1 cut(s) 357
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 1 cut(s) 308
BshTI ACCGGT 1 cut(s) 667
BsiEI CGRYCG 1 cut(s) 256
BsiHKCI CYCGRG 1 cut(s) 784
BsiSI CCGG 7 cut(s) 65, 78, 101, 170, 668, 696, 738
BsmAI GTCTC 3 cut(s) 155, 506, 653
BsmI GAATGC 2 cut(s) 622, 931
BsnI GGCC 1 cut(s) 308
Bso31I GGTCTC 2 cut(s) 155, 506
BsoBI CYCGRG 1 cut(s) 784
Bsp119I TTCGAA 1 cut(s) 407
Bsp13I TCCGGA 1 cut(s) 737
Bsp143I GATC 6 cut(s) 217, 223, 228, 253, 364, 553
BspACI CCGC 9 cut(s) 75, 167, 294, 321, 383, 452, 520, 684, 837
BspANI GGCC 1 cut(s) 308
BspCNI CTCAG 3 cut(s) 522, 613, 617
BspEI TCCGGA 1 cut(s) 737
BspFNI CGCG 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 190
BspQI GCTCTTC 1 cut(s) 960
BspT104I TTCGAA 1 cut(s) 407
BspTNI GGTCTC 2 cut(s) 155, 506
BsrBI CCGCTC 2 cut(s) 294, 520
BsrFI RCCGGY 1 cut(s) 667
BsrI ACTGG 1 cut(s) 51
BssAI RCCGGY 1 cut(s) 667
BssECI CCNNGG 3 cut(s) 126, 195, 538
BssMI GATC 6 cut(s) 217, 223, 228, 253, 364, 553
BssNI GRCGYC 2 cut(s) 9, 297
Bst2UI CCWGG 2 cut(s) 149, 197
Bst4CI ACNGT 4 cut(s) 107, 338, 880, 959
Bst6I CTCTTC 3 cut(s) 404, 651, 960
BstACI GRCGYC 2 cut(s) 9, 297
BstBI TTCGAA 1 cut(s) 407
BstDEI CTNAG 4 cut(s) 524, 530, 604, 621
BstF5I GGATG 3 cut(s) 124, 583, 692
BstFNI CGCG 1 cut(s) 357
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 2 cut(s) 143, 357
BstKTI GATC 6 cut(s) 220, 226, 231, 256, 367, 556
BstMAI GTCTC 3 cut(s) 155, 506, 653
BstMBI GATC 6 cut(s) 217, 223, 228, 253, 364, 553
BstMCI CGRYCG 1 cut(s) 256
BstMWI GCNNNNNNNGC 5 cut(s) 74, 330, 540, 696, 751
BstNI CCWGG 2 cut(s) 149, 197
BstSCI CCNGG 3 cut(s) 99, 147, 195
BstUI CGCG 1 cut(s) 357
BstV1I GCAGC 5 cut(s) 134, 150, 741, 982, 985
BstV2I GAAGAC 3 cut(s) 209, 438, 852
BsuRI GGCC 1 cut(s) 308
BtgZI GCGATG 2 cut(s) 249, 372
BtsCI GGATG 3 cut(s) 124, 583, 692
BtsI GCAGTG 1 cut(s) 730
BtsIMutI CAGTG 1 cut(s) 730
CaiI CAGNNNCTG 1 cut(s) 979
CfoI GCGC 2 cut(s) 143, 357
Cfr10I RCCGGY 1 cut(s) 667
CseI GACGC 2 cut(s) 17, 286
Csp6I GTAC 2 cut(s) 30, 54
CspAI ACCGGT 1 cut(s) 667
CviAII CATG 1 cut(s) 88
CviJI RGCY 8 cut(s) 125, 308, 523, 543, 603, 690, 801, 840
CviKI_1 RGCY 8 cut(s) 125, 308, 523, 543, 603, 690, 801, 840
CviQI GTAC 2 cut(s) 30, 54
DdeI CTNAG 4 cut(s) 524, 530, 604, 621
DpnI GATC 6 cut(s) 219, 225, 230, 255, 366, 555
DpnII GATC 6 cut(s) 217, 223, 228, 253, 364, 553
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 3 cut(s) 404, 651, 960
EarI CTCTTC 3 cut(s) 404, 651, 960
EciI GGCGGA 2 cut(s) 64, 398
Eco31I GGTCTC 2 cut(s) 155, 506
Eco57I CTGAAG 2 cut(s) 675, 696
Eco88I CYCGRG 1 cut(s) 784
EcoRII CCWGG 2 cut(s) 147, 195
FaeI CATG 1 cut(s) 91
FaiI YATR 3 cut(s) 89, 281, 798
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FatI CATG 1 cut(s) 87
FauI CCCGC 1 cut(s) 174
FbaI TGATCA 1 cut(s) 223
FokI GGATG 3 cut(s) 131, 590, 679
FspBI CTAG 2 cut(s) 27, 807
GlaI GCGC 2 cut(s) 142, 356
GsaI CCCAGC 2 cut(s) 779, 947
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 1 cut(s) 308
HapII CCGG 7 cut(s) 65, 78, 101, 170, 668, 696, 738
HgaI GACGC 2 cut(s) 17, 286
HhaI GCGC 2 cut(s) 143, 357
Hin1I GRCGYC 2 cut(s) 9, 297
Hin1II CATG 1 cut(s) 91
Hin6I GCGC 2 cut(s) 141, 355
HinP1I GCGC 2 cut(s) 141, 355
HpaII CCGG 7 cut(s) 65, 78, 101, 170, 668, 696, 738
HphI GGTGA 1 cut(s) 382
Hpy188III TCNNGA 7 cut(s) 160, 221, 257, 563, 606, 716, 738
Hpy99I CGWCG 8 cut(s) 11, 245, 248, 251, 254, 314, 338, 460
HpyAV CCTTC 5 cut(s) 323, 422, 713, 841, 903
HpyCH4III ACNGT 4 cut(s) 107, 338, 880, 959
HpyCH4IV ACGT 2 cut(s) 153, 829
HpyCH4V TGCA 2 cut(s) 122, 572
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 330, 540, 696, 751
HpyF3I CTNAG 4 cut(s) 524, 530, 604, 621
HpySE526I ACGT 2 cut(s) 153, 829
Hsp92I GRCGYC 2 cut(s) 9, 297
Hsp92II CATG 1 cut(s) 91
HspAI GCGC 2 cut(s) 141, 355
Kpn2I TCCGGA 1 cut(s) 737
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 6 cut(s) 217, 223, 228, 253, 364, 553
LguI GCTCTTC 1 cut(s) 960
LmnI GCTCC 2 cut(s) 291, 478
Lsp1109I GCAGC 5 cut(s) 134, 150, 741, 982, 985
LweI GCATC 3 cut(s) 109, 349, 571
MaeI CTAG 2 cut(s) 27, 807
MaeII ACGT 2 cut(s) 153, 829
MaeIII GTNAC 2 cut(s) 370, 670
MalI GATC 6 cut(s) 219, 225, 230, 255, 366, 555
MbiI CCGCTC 2 cut(s) 294, 520
MboI GATC 6 cut(s) 217, 223, 228, 253, 364, 553
MluCI AATT 6 cut(s) 395, 464, 506, 567, 729, 866
MlyI GAGTC 7 cut(s) 29, 182, 199, 452, 655, 776, 866
MmeI TCCRAC 4 cut(s) 166, 253, 795, 902
MroI TCCGGA 1 cut(s) 737
MroXI GAANNNNTTC 1 cut(s) 913
MspI CCGG 7 cut(s) 65, 78, 101, 170, 668, 696, 738
MspR9I CCNGG 3 cut(s) 101, 149, 197
Mva1269I GAATGC 2 cut(s) 622, 931
MvaI CCWGG 2 cut(s) 149, 197
MvnI CGCG 1 cut(s) 357
MwoI GCNNNNNNNGC 5 cut(s) 74, 330, 540, 696, 751
NciI CCSGG 1 cut(s) 101
NdeII GATC 6 cut(s) 217, 223, 228, 253, 364, 553
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 1 cut(s) 190
NmeAIII GCCGAG 2 cut(s) 151, 519
NmuCI GTSAC 1 cut(s) 370
NspV TTCGAA 1 cut(s) 407
PaeR7I CTCGAG 1 cut(s) 784
PciSI GCTCTTC 1 cut(s) 960
PcsI WCGNNNNNNNCGW 5 cut(s) 15, 237, 246, 249, 455
PctI GAATGC 2 cut(s) 622, 931
PdmI GAANNNNTTC 1 cut(s) 913
PfeI GAWTC 6 cut(s) 47, 81, 386, 485, 548, 734
PfoI TCCNGGA 1 cut(s) 99
PinAI ACCGGT 1 cut(s) 667
Ple19I CGATCG 1 cut(s) 256
PleI GAGTC 7 cut(s) 29, 181, 199, 451, 655, 776, 865
PpsI GAGTC 7 cut(s) 29, 181, 199, 451, 655, 776, 865
Psp6I CCWGG 2 cut(s) 147, 195
PspFI CCCAGC 2 cut(s) 775, 943
PspGI CCWGG 2 cut(s) 147, 195
PspN4I GGNNCC 1 cut(s) 190
PspXI VCTCGAGB 1 cut(s) 784
PstNI CAGNNNCTG 1 cut(s) 979
PvuI CGATCG 1 cut(s) 256
RsaI GTAC 2 cut(s) 31, 55
RsaNI GTAC 2 cut(s) 30, 54
SapI GCTCTTC 1 cut(s) 960
Sau3AI GATC 6 cut(s) 217, 223, 228, 253, 364, 553
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 7 cut(s) 29, 182, 199, 452, 655, 776, 866
ScrFI CCNGG 3 cut(s) 101, 149, 197
SetI ASST 7 cut(s) 74, 156, 194, 540, 605, 803, 832
SfaNI GCATC 3 cut(s) 109, 349, 571
Sfr274I CTCGAG 1 cut(s) 784
SfuI TTCGAA 1 cut(s) 407
SlaI CTCGAG 1 cut(s) 784
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
Sse9I AATT 6 cut(s) 395, 464, 506, 567, 729, 866
SsiI CCGC 9 cut(s) 75, 167, 294, 321, 383, 452, 520, 684, 837
SspMI CTAG 2 cut(s) 27, 807
StyD4I CCNGG 3 cut(s) 99, 147, 195
TaaI ACNGT 4 cut(s) 107, 338, 880, 959
TaiI ACGT 2 cut(s) 156, 832
TaqII GACCGA 1 cut(s) 530
TasI AATT 6 cut(s) 395, 464, 506, 567, 729, 866
TatI WGTACW 1 cut(s) 53
TauI GCSGC 5 cut(s) 297, 324, 454, 523, 840
TfiI GAWTC 6 cut(s) 47, 81, 386, 485, 548, 734
TscAI CASTG 1 cut(s) 730
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 5 cut(s) 122, 138, 754, 970, 973
Tsp45I GTSAC 1 cut(s) 370
TspGWI ACGGA 1 cut(s) 754
TspRI CASTG 1 cut(s) 730
XapI RAATTY 1 cut(s) 395
XhoI CTCGAG 1 cut(s) 784
XmnI GAANNNNTTC 1 cut(s) 913
XspI CTAG 2 cut(s) 27, 807
ZrmI AGTACT 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.