Rw7G019050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014747
Physical Location & Seq
Reverse (-)
20825243 .. 20826307
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G019050.1

Sequence Viewer

Length: 1008 bp
ATGGACGACGACTACTCTAGTACCGACTCATCTTCCGATTCCAGTACTACTATTCCGGCAACCTCCGCCGGAATCGTCATGCTCTCTCTCCCGGACAGTTTCGATGAGGATGCAGCCGAGGACAAGAGCAGCGCCGACCAGGACGTTTTCGAGACCCCGCCGGAGTCCTCGCTTGTCGGAACCTCCCAGGAGAAGACTCCCTTGCTCGATCTTGATCAGATCGGCGATGACGACGATCGTGATTTCGAGGGAGTTGGAGATATAGGAGATGGGAGCGGCGTCATCGTGGCCGTCGGTGGTTGCGGCGAGGAAGGCGACGGTGCTGTGGATTTGGGGCGGGATGCTGATCTGGTGACTTCGGAGGCGGAATCGGTGGAATTTAGGGTTTCGAAGAGGGATTTGTCGATGGAAGGGAGTTCGGAGGAGTCTTCGCCGCCGTCGAAGAAATTGAGGGTTTCGGAGCAGGAATCGGGGAAATCAGAGGAGGAATTGGAGACCGAGCGGCTCAGTCTGAGCAACCTCGGCTCTGATTCCGATCAAACTCTCGAAATTGCAGGGGATGATGCCAAATCAGTTGAGGAAAGCTCAGGAAAGAAGAATGCTGAGTATTCAGACAGCGAGAGTGAGAATGCTACTGAAGAGACTCAACCGGTTACTGAAGAAAGCGGAAGCCCATCCGGCGTGGAGTATTCCTATCAGGAAGGCAGTGAAATTGATTCCGGAGTGCTTAGAGTGCTGCCTCTATCCTTCTGCCCACCAGCAGACTCGAGTGAGGGGCATAGCTGGTCTAGTCTGTTGGAGATGTTGAAACGTGTGAGAGAAATAACTCTTCAGACCGTAAAAATGAGCTGTAGAGTGGCGGCTGGAAGAAGGGTTTTGATTCTTCTGAATTGGACGAAACCGTATTCACAAGAAACGAAGAAGATGAGGGAAGGATTTCGGGTTGGAATGCTGGGTTTTGAGGACAGTCTTGAAGAGCAGCAGCGACTGAAAATCCCAGGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

335

Amino Acids

36.07

Weight (kDa)

4.12

Isoelectric Point (pI)

61.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 276, 502
AccIII TCCGGA 1 cut(s) 719
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 3 cut(s) 657, 678, 815
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 2 cut(s) 22, 46
AflIII ACRYGT 1 cut(s) 811
AgeI ACCGGT 1 cut(s) 649
AgsI TTSAA 2 cut(s) 808, 974
AjnI CCWGG 3 cut(s) 138, 186, 997
AluBI AGCT 3 cut(s) 585, 783, 849
AluI AGCT 3 cut(s) 585, 783, 849
Alw26I GTCTC 3 cut(s) 146, 488, 635
AlwNI CAGNNNCTG 1 cut(s) 988
Ama87I CYCGRG 1 cut(s) 766
Aor13HI TCCGGA 1 cut(s) 719
AoxI GGCC 1 cut(s) 288
ApeKI GCWGC 5 cut(s) 113, 129, 736, 979, 982
ApoI RAATTY 1 cut(s) 377
AsiGI ACCGGT 1 cut(s) 649
Asp700I GAANNNNTTC 1 cut(s) 936
AspLEI GCGC 1 cut(s) 134
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 364
AsuII TTCGAA 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 766
BaeI ACNNNNGTAYC 1 cut(s) 37
BbsI GAAGAC 2 cut(s) 200, 420
BbvI GCAGC 5 cut(s) 125, 141, 723, 991, 994
BccI CCATC 3 cut(s) 263, 400, 682
BceAI ACGGC 2 cut(s) 275, 421
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 3 cut(s) 140, 188, 999
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 3 cut(s) 146, 488, 635
BfaI CTAG 2 cut(s) 18, 789
BfmI CTRYAG 1 cut(s) 850
BfoI RGCGCY 1 cut(s) 135
BglI GCCNNNNNGGC 1 cut(s) 678
BmcAI AGTACT 1 cut(s) 46
Bme1390I CCNGG 4 cut(s) 92, 140, 188, 999
BmeT110I CYCGRG 1 cut(s) 766
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 4 cut(s) 92, 140, 188, 999
BmsI GCATC 3 cut(s) 100, 331, 553
BpiI GAAGAC 2 cut(s) 200, 420
BplI GAGNNNNNCTC 2 cut(s) 569, 601
Bpu10I CCTNAGC 1 cut(s) 586
Bpu14I TTCGAA 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 92
BsaBI GATNNNNATC 3 cut(s) 213, 345, 534
BsaHI GRCGYC 1 cut(s) 279
BsaI GGTCTC 2 cut(s) 146, 488
BsaJI CCNNGG 4 cut(s) 117, 186, 520, 997
BsaWI WCCGGW 2 cut(s) 649, 719
Bse118I RCCGGY 1 cut(s) 649
Bse1I ACTGG 1 cut(s) 42
Bse8I GATNNNNATC 3 cut(s) 213, 345, 534
BseAI TCCGGA 1 cut(s) 719
BseBI CCWGG 3 cut(s) 140, 188, 999
BseDI CCNNGG 4 cut(s) 117, 186, 520, 997
BseGI GGATG 4 cut(s) 115, 346, 565, 674
BseJI GATNNNNATC 3 cut(s) 213, 345, 534
BseMII CTCAG 4 cut(s) 503, 520, 594, 600
BseNI ACTGG 1 cut(s) 42
BseRI GAGGAG 2 cut(s) 437, 497
BseXI GCAGC 5 cut(s) 125, 141, 723, 991, 994
BseYI CCCAGC 1 cut(s) 952
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 1 cut(s) 290
BshTI ACCGGT 1 cut(s) 649
BsiEI CGRYCG 1 cut(s) 238
BsiHKCI CYCGRG 1 cut(s) 766
BsiSI CCGG 7 cut(s) 56, 69, 92, 161, 650, 678, 720
BsmAI GTCTC 3 cut(s) 146, 488, 635
BsmI GAATGC 3 cut(s) 604, 634, 954
BsnI GGCC 1 cut(s) 290
Bso31I GGTCTC 2 cut(s) 146, 488
BsoBI CYCGRG 1 cut(s) 766
Bsp119I TTCGAA 1 cut(s) 389
Bsp13I TCCGGA 1 cut(s) 719
Bsp143I GATC 6 cut(s) 208, 214, 219, 235, 346, 535
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 4 cut(s) 504, 519, 595, 599
BspEI TCCGGA 1 cut(s) 719
BspLI GGNNCC 1 cut(s) 181
BspQI GCTCTTC 1 cut(s) 969
BspT104I TTCGAA 1 cut(s) 389
BspTNI GGTCTC 2 cut(s) 146, 488
BsrBI CCGCTC 2 cut(s) 276, 502
BsrFI RCCGGY 1 cut(s) 649
BsrI ACTGG 1 cut(s) 42
BssAI RCCGGY 1 cut(s) 649
BssECI CCNNGG 4 cut(s) 117, 186, 520, 997
BssMI GATC 6 cut(s) 208, 214, 219, 235, 346, 535
BssNI GRCGYC 1 cut(s) 279
Bst2UI CCWGG 3 cut(s) 140, 188, 999
Bst4CI ACNGT 5 cut(s) 98, 320, 838, 903, 968
Bst6I CTCTTC 4 cut(s) 386, 633, 834, 969
BstACI GRCGYC 1 cut(s) 279
BstBI TTCGAA 1 cut(s) 389
BstDEI CTNAG 5 cut(s) 506, 512, 586, 603, 728
BstF5I GGATG 4 cut(s) 115, 346, 565, 674
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 6 cut(s) 211, 217, 222, 238, 349, 538
BstMAI GTCTC 3 cut(s) 146, 488, 635
BstMBI GATC 6 cut(s) 208, 214, 219, 235, 346, 535
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 5 cut(s) 65, 312, 522, 678, 733
BstNI CCWGG 3 cut(s) 140, 188, 999
BstSCI CCNGG 4 cut(s) 90, 138, 186, 997
BstSFI CTRYAG 1 cut(s) 850
BstV1I GCAGC 5 cut(s) 125, 141, 723, 991, 994
BstV2I GAAGAC 2 cut(s) 200, 420
BsuRI GGCC 1 cut(s) 290
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 4 cut(s) 115, 346, 565, 674
BtsI GCAGTG 1 cut(s) 712
BtsIMutI CAGTG 1 cut(s) 712
CaiI CAGNNNCTG 1 cut(s) 988
CfoI GCGC 1 cut(s) 134
Cfr10I RCCGGY 1 cut(s) 649
CseI GACGC 1 cut(s) 268
Csp6I GTAC 2 cut(s) 21, 45
CspAI ACCGGT 1 cut(s) 649
CviAII CATG 1 cut(s) 79
CviJI RGCY 9 cut(s) 116, 290, 505, 525, 585, 672, 783, 849, 863
CviKI_1 RGCY 9 cut(s) 116, 290, 505, 525, 585, 672, 783, 849, 863
CviQI GTAC 2 cut(s) 21, 45
DdeI CTNAG 5 cut(s) 506, 512, 586, 603, 728
DpnI GATC 6 cut(s) 210, 216, 221, 237, 348, 537
DpnII GATC 6 cut(s) 208, 214, 219, 235, 346, 535
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 4 cut(s) 386, 633, 834, 969
EarI CTCTTC 4 cut(s) 386, 633, 834, 969
EciI GGCGGA 2 cut(s) 55, 380
Eco31I GGTCTC 2 cut(s) 146, 488
Eco57I CTGAAG 3 cut(s) 657, 678, 815
Eco88I CYCGRG 1 cut(s) 766
EcoRII CCWGG 3 cut(s) 138, 186, 997
FaeI CATG 1 cut(s) 82
FaiI YATR 3 cut(s) 80, 263, 780
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FatI CATG 1 cut(s) 78
FauI CCCGC 2 cut(s) 165, 330
FbaI TGATCA 1 cut(s) 214
FokI GGATG 4 cut(s) 122, 353, 572, 661
FspBI CTAG 2 cut(s) 18, 789
GlaI GCGC 1 cut(s) 133
GsaI CCCAGC 1 cut(s) 956
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 1 cut(s) 290
HapII CCGG 7 cut(s) 56, 69, 92, 161, 650, 678, 720
HgaI GACGC 1 cut(s) 268
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 1 cut(s) 82
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HpaII CCGG 7 cut(s) 56, 69, 92, 161, 650, 678, 720
HphI GGTGA 1 cut(s) 364
Hpy188III TCNNGA 8 cut(s) 151, 212, 239, 545, 588, 698, 720, 971
Hpy99I CGWCG 5 cut(s) 11, 236, 296, 320, 442
HpyAV CCTTC 6 cut(s) 305, 404, 695, 757, 864, 926
HpyCH4III ACNGT 5 cut(s) 98, 320, 838, 903, 968
HpyCH4IV ACGT 2 cut(s) 144, 811
HpyCH4V TGCA 2 cut(s) 113, 554
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 312, 522, 678, 733
HpyF3I CTNAG 5 cut(s) 506, 512, 586, 603, 728
HpySE526I ACGT 2 cut(s) 144, 811
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 1 cut(s) 82
HspAI GCGC 1 cut(s) 132
Kpn2I TCCGGA 1 cut(s) 719
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 6 cut(s) 208, 214, 219, 235, 346, 535
LguI GCTCTTC 1 cut(s) 969
LmnI GCTCC 2 cut(s) 273, 460
Lsp1109I GCAGC 5 cut(s) 125, 141, 723, 991, 994
LweI GCATC 3 cut(s) 100, 331, 553
MaeI CTAG 2 cut(s) 18, 789
MaeII ACGT 2 cut(s) 144, 811
MaeIII GTNAC 2 cut(s) 352, 652
MalI GATC 6 cut(s) 210, 216, 221, 237, 348, 537
MbiI CCGCTC 2 cut(s) 276, 502
MboI GATC 6 cut(s) 208, 214, 219, 235, 346, 535
MluCI AATT 6 cut(s) 377, 446, 488, 549, 711, 889
MlyI GAGTC 6 cut(s) 20, 173, 190, 434, 637, 758
MmeI TCCRAC 4 cut(s) 157, 235, 777, 925
MroI TCCGGA 1 cut(s) 719
MroXI GAANNNNTTC 1 cut(s) 936
MseI TTAA 1 cut(s) 1006
MspI CCGG 7 cut(s) 56, 69, 92, 161, 650, 678, 720
MspR9I CCNGG 4 cut(s) 92, 140, 188, 999
Mva1269I GAATGC 3 cut(s) 604, 634, 954
MvaI CCWGG 3 cut(s) 140, 188, 999
MwoI GCNNNNNNNGC 5 cut(s) 65, 312, 522, 678, 733
NciI CCSGG 1 cut(s) 92
NdeII GATC 6 cut(s) 208, 214, 219, 235, 346, 535
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 1 cut(s) 181
NmeAIII GCCGAG 2 cut(s) 142, 501
NmuCI GTSAC 1 cut(s) 352
NspV TTCGAA 1 cut(s) 389
PaeR7I CTCGAG 1 cut(s) 766
PciSI GCTCTTC 1 cut(s) 969
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 437
PctI GAATGC 3 cut(s) 604, 634, 954
PdmI GAANNNNTTC 1 cut(s) 936
PfeI GAWTC 7 cut(s) 38, 72, 368, 467, 530, 716, 880
PfoI TCCNGGA 1 cut(s) 90
PinAI ACCGGT 1 cut(s) 649
Ple19I CGATCG 1 cut(s) 238
PleI GAGTC 6 cut(s) 20, 172, 190, 433, 637, 758
PpsI GAGTC 6 cut(s) 20, 172, 190, 433, 637, 758
Psp6I CCWGG 3 cut(s) 138, 186, 997
PspFI CCCAGC 1 cut(s) 952
PspGI CCWGG 3 cut(s) 138, 186, 997
PspN4I GGNNCC 1 cut(s) 181
PspXI VCTCGAGB 1 cut(s) 766
PstNI CAGNNNCTG 1 cut(s) 988
PvuI CGATCG 1 cut(s) 238
RsaI GTAC 2 cut(s) 22, 46
RsaNI GTAC 2 cut(s) 21, 45
SapI GCTCTTC 1 cut(s) 969
SaqAI TTAA 1 cut(s) 1006
Sau3AI GATC 6 cut(s) 208, 214, 219, 235, 346, 535
ScaI AGTACT 1 cut(s) 46
SchI GAGTC 6 cut(s) 20, 173, 190, 434, 637, 758
ScrFI CCNGG 4 cut(s) 92, 140, 188, 999
SetI ASST 9 cut(s) 65, 147, 185, 522, 587, 785, 814, 851, 1003
SfaNI GCATC 3 cut(s) 100, 331, 553
SfcI CTRYAG 1 cut(s) 850
Sfr274I CTCGAG 1 cut(s) 766
SfuI TTCGAA 1 cut(s) 389
SlaI CTCGAG 1 cut(s) 766
SmlI CTYRAG 1 cut(s) 766
SmoI CTYRAG 1 cut(s) 766
Sse9I AATT 6 cut(s) 377, 446, 488, 549, 711, 889
SspMI CTAG 2 cut(s) 18, 789
StyD4I CCNGG 4 cut(s) 90, 138, 186, 997
TaaI ACNGT 5 cut(s) 98, 320, 838, 903, 968
TaiI ACGT 2 cut(s) 147, 814
TaqI TCGA 9 cut(s) 102, 150, 207, 246, 389, 404, 440, 546, 767
TaqII GACCGA 1 cut(s) 512
TasI AATT 6 cut(s) 377, 446, 488, 549, 711, 889
TatI WGTACW 1 cut(s) 44
TauI GCSGC 5 cut(s) 279, 306, 436, 505, 863
TfiI GAWTC 7 cut(s) 38, 72, 368, 467, 530, 716, 880
Tru1I TTAA 1 cut(s) 1006
Tru9I TTAA 1 cut(s) 1006
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 5 cut(s) 113, 129, 736, 979, 982
Tsp45I GTSAC 1 cut(s) 352
TspRI CASTG 1 cut(s) 712
XapI RAATTY 1 cut(s) 377
XhoI CTCGAG 1 cut(s) 766
XmnI GAANNNNTTC 1 cut(s) 936
XspI CTAG 2 cut(s) 18, 789
ZrmI AGTACT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.