Rroxscaffold_3G00252470

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014747
Physical Location & Seq
Forward (+)
46491895 .. 46492845
951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252470.1

Sequence Viewer

Length: 951 bp
ATGGACGACGCCGACGACGACTACGACTACTCCAGTACCGACTCGTCTTCCGATTCCAGTACTACTATTCCGGAAGCCTCCGCCGGAATCGTCATGCTCACTCTCCCGGACAGTTTCGATGAGGATGCGGCCGAGGACAAGAGCAGCGCCGACCAGGACGTTTTCGAGACCCCGCCGGAGTCCGCGCTCGTCGGAACCTCCCAGGAGAAGACTCCCTTGCTCGACCTTGATCAGATCGGCAAAGACGACGACGACGACGATGATCGTGATTTCGAGGGAGTTGGAGATATAGGAGATGGGAGCGGCGTCATCGTGGCCGTCGGTGGTTGCGGCGAGGAAGGCGACGGTGCTGTGGATTTGGGGCGGGATGCTGATCTGGTGACTTCGGAGGCGGAATCGGTGGAATTTATGGTTTCGAAGAGGGGTTTGTCGATGGAAGGGAGTTCGGAGGAGTCTTCGCCGCCGTCGAAGAAATTGAGGGTTTCGGAGCAGGAATCGGGGAAATCAGAGGAGGAATTGGAGACCGAGCGGCTTAGTCTGAGCAAACTCGGCTCTGATTCCGATCAAACTCTCGAAATTGCAGGGGATGATGCCAAATCAGTAGAGGAAAGCTCAGGAAAGAAGAATGCTGAGTATTCAGACAGCGAGAGTGAGAATGCTACTCAAGAGACTCAACAGGTTACTGAAGAAAGCGGTAGCCCATCCAGCGTGGAGTATTCCTATCAGGAAGGCAGTGAAATTGGTTCCGGAGATTCGCGAGTGCTTCGAGTGCTGCCTCTATCCTTCTTCTGCCCACCAGCAGACTCGAGTGAGATGCATAGCTGGTCTAGTCTGTTGGAGATGTTGAAACGTGTGGGAGAAATAACTCGTCAAGACTGTAAAAATGAGGTTGATAAATGTAGCTTTTTGGAGATAGCTAAGCGTCGCGGCATGACTTTCCCTCAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

316

Amino Acids

33.9

Weight (kDa)

4.05

Isoelectric Point (pI)

58.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 303, 529
AccII CGCG 3 cut(s) 185, 757, 927
AccIII TCCGGA 2 cut(s) 70, 746
AcoI YGGCCR 2 cut(s) 129, 315
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 1 cut(s) 705
AcyI GRCGYC 2 cut(s) 9, 306
AfaI GTAC 2 cut(s) 37, 61
AflIII ACRYGT 1 cut(s) 850
AgsI TTSAA 1 cut(s) 847
AjnI CCWGG 2 cut(s) 153, 201
AluBI AGCT 5 cut(s) 612, 822, 903, 917, 946
AluI AGCT 5 cut(s) 612, 822, 903, 917, 946
Alw26I GTCTC 3 cut(s) 161, 515, 662
Ama87I CYCGRG 1 cut(s) 805
Aor13HI TCCGGA 2 cut(s) 70, 746
AoxI GGCC 2 cut(s) 129, 315
ApeKI GCWGC 2 cut(s) 144, 772
ApoI RAATTY 1 cut(s) 404
AspLEI GCGC 2 cut(s) 149, 187
AsuC2I CCSGG 1 cut(s) 107
AsuHPI GGTGA 1 cut(s) 391
AsuII TTCGAA 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 805
BaeI ACNNNNGTAYC 2 cut(s) 19, 52
BbsI GAAGAC 3 cut(s) 39, 215, 447
BbvCI CCTCAGC 1 cut(s) 942
BbvI GCAGC 2 cut(s) 156, 759
BccI CCATC 3 cut(s) 290, 427, 709
BceAI ACGGC 2 cut(s) 302, 448
BcgI CGANNNNNNTGC 4 cut(s) 107, 141, 796, 830
BciT130I CCWGG 2 cut(s) 155, 203
BclI TGATCA 1 cut(s) 229
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 3 cut(s) 161, 515, 662
BfaI CTAG 1 cut(s) 828
BfmI CTRYAG 1 cut(s) 947
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 8 cut(s) 129, 145, 304, 331, 461, 530, 773, 928
BlpI GCTNAGC 1 cut(s) 918
BlsI GCNGC 8 cut(s) 130, 146, 305, 332, 462, 531, 774, 929
BmcAI AGTACT 1 cut(s) 61
Bme1390I CCNGG 3 cut(s) 107, 155, 203
BmeT110I CYCGRG 1 cut(s) 805
BmiI GGNNCC 2 cut(s) 196, 745
BmrFI CCNGG 3 cut(s) 107, 155, 203
BmsI GCATC 4 cut(s) 115, 358, 580, 804
BpiI GAAGAC 3 cut(s) 39, 215, 447
BplI GAGNNNNNCTC 2 cut(s) 596, 628
BpmI CTGGAG 1 cut(s) 16
Bpu10I CCTNAGC 2 cut(s) 613, 942
Bpu1102I GCTNAGC 1 cut(s) 918
Bpu14I TTCGAA 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 648
BpuMI CCSGG 1 cut(s) 107
BsaBI GATNNNNATC 2 cut(s) 372, 561
BsaHI GRCGYC 2 cut(s) 9, 306
BsaI GGTCTC 2 cut(s) 161, 515
BsaJI CCNNGG 2 cut(s) 132, 201
BsaWI WCCGGW 2 cut(s) 70, 746
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bse1I ACTGG 2 cut(s) 33, 57
Bse8I GATNNNNATC 2 cut(s) 372, 561
BseAI TCCGGA 2 cut(s) 70, 746
BseBI CCWGG 2 cut(s) 155, 203
BseDI CCNNGG 2 cut(s) 132, 201
BseGI GGATG 4 cut(s) 130, 373, 592, 701
BseJI GATNNNNATC 2 cut(s) 372, 561
BseMII CTCAG 3 cut(s) 530, 621, 627
BseNI ACTGG 2 cut(s) 33, 57
BseRI GAGGAG 2 cut(s) 464, 524
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 2 cut(s) 156, 759
Bsh1236I CGCG 3 cut(s) 185, 757, 927
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 2 cut(s) 131, 317
BsiEI CGRYCG 1 cut(s) 132
BsiHKCI CYCGRG 1 cut(s) 805
BsiSI CCGG 5 cut(s) 71, 84, 107, 176, 747
BsmAI GTCTC 3 cut(s) 161, 515, 662
BsmI GAATGC 2 cut(s) 631, 661
BsnI GGCC 2 cut(s) 131, 317
Bso31I GGTCTC 2 cut(s) 161, 515
BsoBI CYCGRG 1 cut(s) 805
Bsp119I TTCGAA 1 cut(s) 416
Bsp13I TCCGGA 2 cut(s) 70, 746
Bsp143I GATC 5 cut(s) 229, 234, 262, 373, 562
Bsp1720I GCTNAGC 1 cut(s) 918
Bsp68I TCGCGA 1 cut(s) 757
BspANI GGCC 2 cut(s) 131, 317
BspCNI CTCAG 3 cut(s) 531, 622, 626
BspEI TCCGGA 2 cut(s) 70, 746
BspFNI CGCG 3 cut(s) 185, 757, 927
BspLI GGNNCC 2 cut(s) 196, 745
BspT104I TTCGAA 1 cut(s) 416
BspTNI GGTCTC 2 cut(s) 161, 515
BsrBI CCGCTC 2 cut(s) 303, 529
BsrI ACTGG 2 cut(s) 33, 57
BssECI CCNNGG 2 cut(s) 132, 201
BssMI GATC 5 cut(s) 229, 234, 262, 373, 562
BssNI GRCGYC 2 cut(s) 9, 306
Bst2UI CCWGG 2 cut(s) 155, 203
Bst4CI ACNGT 3 cut(s) 113, 347, 878
Bst6I CTCTTC 1 cut(s) 413
BstACI GRCGYC 2 cut(s) 9, 306
BstBI TTCGAA 1 cut(s) 416
BstDEI CTNAG 6 cut(s) 533, 539, 613, 630, 918, 942
BstF5I GGATG 4 cut(s) 130, 373, 592, 701
BstFNI CGCG 3 cut(s) 185, 757, 927
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 187
BstKTI GATC 5 cut(s) 232, 237, 265, 376, 565
BstMAI GTCTC 3 cut(s) 161, 515, 662
BstMBI GATC 5 cut(s) 229, 234, 262, 373, 562
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 4 cut(s) 339, 549, 705, 769
BstNI CCWGG 2 cut(s) 155, 203
BstSCI CCNGG 3 cut(s) 105, 153, 201
BstSFI CTRYAG 1 cut(s) 947
BstUI CGCG 3 cut(s) 185, 757, 927
BstV1I GCAGC 2 cut(s) 156, 759
BstV2I GAAGAC 3 cut(s) 39, 215, 447
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 2 cut(s) 131, 317
BtsCI GGATG 4 cut(s) 130, 373, 592, 701
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 1 cut(s) 739
BtuMI TCGCGA 1 cut(s) 757
CfoI GCGC 2 cut(s) 149, 187
CseI GACGC 3 cut(s) 17, 295, 911
Csp6I GTAC 2 cut(s) 36, 60
CviAII CATG 2 cut(s) 94, 931
CviQI GTAC 2 cut(s) 36, 60
DdeI CTNAG 6 cut(s) 533, 539, 613, 630, 918, 942
DpnI GATC 5 cut(s) 231, 236, 264, 375, 564
DpnII GATC 5 cut(s) 229, 234, 262, 373, 562
EaeI YGGCCR 2 cut(s) 129, 315
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
EciI GGCGGA 2 cut(s) 70, 407
EclXI CGGCCG 1 cut(s) 129
Eco31I GGTCTC 2 cut(s) 161, 515
Eco52I CGGCCG 1 cut(s) 129
Eco57I CTGAAG 1 cut(s) 705
Eco88I CYCGRG 1 cut(s) 805
EcoRII CCWGG 2 cut(s) 153, 201
EcoT22I ATGCAT 1 cut(s) 819
FaeI CATG 2 cut(s) 97, 934
FaiI YATR 5 cut(s) 95, 290, 410, 819, 932
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FatI CATG 2 cut(s) 93, 930
FauI CCCGC 2 cut(s) 180, 357
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 8 cut(s) 129, 145, 304, 331, 461, 530, 773, 928
FokI GGATG 4 cut(s) 137, 380, 599, 688
Fsp4HI GCNGC 8 cut(s) 129, 145, 304, 331, 461, 530, 773, 928
FspBI CTAG 1 cut(s) 828
GlaI GCGC 2 cut(s) 148, 186
GluI GCNGC 8 cut(s) 129, 145, 304, 331, 461, 530, 773, 928
GsuI CTGGAG 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 131, 317
HapII CCGG 5 cut(s) 71, 84, 107, 176, 747
HgaI GACGC 3 cut(s) 17, 295, 911
HhaI GCGC 2 cut(s) 149, 187
Hin1I GRCGYC 2 cut(s) 9, 306
Hin1II CATG 2 cut(s) 97, 934
Hin6I GCGC 2 cut(s) 147, 185
HinP1I GCGC 2 cut(s) 147, 185
HpaII CCGG 5 cut(s) 71, 84, 107, 176, 747
HphI GGTGA 1 cut(s) 391
HpyAV CCTTC 4 cut(s) 332, 431, 722, 793
HpyCH4III ACNGT 3 cut(s) 113, 347, 878
HpyCH4IV ACGT 2 cut(s) 159, 850
HpyCH4V TGCA 2 cut(s) 581, 817
HpyF10VI GCNNNNNNNGC 4 cut(s) 339, 549, 705, 769
HpyF3I CTNAG 6 cut(s) 533, 539, 613, 630, 918, 942
HpySE526I ACGT 2 cut(s) 159, 850
Hsp92I GRCGYC 2 cut(s) 9, 306
Hsp92II CATG 2 cut(s) 97, 934
HspAI GCGC 2 cut(s) 147, 185
Kpn2I TCCGGA 2 cut(s) 70, 746
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 5 cut(s) 229, 234, 262, 373, 562
LmnI GCTCC 2 cut(s) 300, 487
Lsp1109I GCAGC 2 cut(s) 156, 759
LweI GCATC 4 cut(s) 115, 358, 580, 804
MaeI CTAG 1 cut(s) 828
MaeII ACGT 2 cut(s) 159, 850
MaeIII GTNAC 2 cut(s) 379, 679
MalI GATC 5 cut(s) 231, 236, 264, 375, 564
MbiI CCGCTC 2 cut(s) 303, 529
MboI GATC 5 cut(s) 229, 234, 262, 373, 562
MboII GAAGA 8 cut(s) 39, 220, 430, 447, 481, 634, 698, 778
MluCI AATT 5 cut(s) 404, 473, 515, 576, 738
MlyI GAGTC 6 cut(s) 35, 188, 205, 461, 664, 797
MmeI TCCRAC 3 cut(s) 172, 262, 816
Mph1103I ATGCAT 1 cut(s) 819
MroI TCCGGA 2 cut(s) 70, 746
MspA1I CMGCKG 1 cut(s) 946
MspI CCGG 5 cut(s) 71, 84, 107, 176, 747
MspR9I CCNGG 3 cut(s) 107, 155, 203
Mva1269I GAATGC 2 cut(s) 631, 661
MvaI CCWGG 2 cut(s) 155, 203
MvnI CGCG 3 cut(s) 185, 757, 927
MwoI GCNNNNNNNGC 4 cut(s) 339, 549, 705, 769
NciI CCSGG 1 cut(s) 107
NdeII GATC 5 cut(s) 229, 234, 262, 373, 562
NlaIII CATG 2 cut(s) 97, 934
NlaIV GGNNCC 2 cut(s) 196, 745
NmeAIII GCCGAG 2 cut(s) 157, 528
NmuCI GTSAC 1 cut(s) 379
NruI TCGCGA 1 cut(s) 757
NsiI ATGCAT 1 cut(s) 819
NspV TTCGAA 1 cut(s) 416
PaeR7I CTCGAG 1 cut(s) 805
PcsI WCGNNNNNNNCGW 7 cut(s) 12, 15, 21, 243, 252, 255, 464
PctI GAATGC 2 cut(s) 631, 661
PfeI GAWTC 6 cut(s) 53, 87, 395, 494, 557, 752
PfoI TCCNGGA 1 cut(s) 105
PkrI GCNGC 8 cut(s) 130, 146, 305, 332, 462, 531, 774, 929
PleI GAGTC 6 cut(s) 35, 187, 205, 460, 664, 797
PpsI GAGTC 6 cut(s) 35, 187, 205, 460, 664, 797
Psp6I CCWGG 2 cut(s) 153, 201
PspGI CCWGG 2 cut(s) 153, 201
PspN4I GGNNCC 2 cut(s) 196, 745
PspXI VCTCGAGB 1 cut(s) 805
PvuII CAGCTG 1 cut(s) 946
RruI TCGCGA 1 cut(s) 757
RsaI GTAC 2 cut(s) 37, 61
RsaNI GTAC 2 cut(s) 36, 60
SatI GCNGC 8 cut(s) 129, 145, 304, 331, 461, 530, 773, 928
Sau3AI GATC 5 cut(s) 229, 234, 262, 373, 562
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 6 cut(s) 35, 188, 205, 461, 664, 797
ScrFI CCNGG 3 cut(s) 107, 155, 203
SfaNI GCATC 4 cut(s) 115, 358, 580, 804
SfcI CTRYAG 1 cut(s) 947
Sfr274I CTCGAG 1 cut(s) 805
SfuI TTCGAA 1 cut(s) 416
SlaI CTCGAG 1 cut(s) 805
SmlI CTYRAG 2 cut(s) 663, 805
SmoI CTYRAG 2 cut(s) 663, 805
Sse9I AATT 5 cut(s) 404, 473, 515, 576, 738
SspMI CTAG 1 cut(s) 828
StyD4I CCNGG 3 cut(s) 105, 153, 201
TaaI ACNGT 3 cut(s) 113, 347, 878
TaiI ACGT 2 cut(s) 162, 853
TaqII GACCGA 1 cut(s) 539
TasI AATT 5 cut(s) 404, 473, 515, 576, 738
TatI WGTACW 1 cut(s) 59
TauI GCSGC 6 cut(s) 131, 306, 333, 463, 532, 930
TfiI GAWTC 6 cut(s) 53, 87, 395, 494, 557, 752
TscAI CASTG 1 cut(s) 739
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 2 cut(s) 144, 772
Tsp45I GTSAC 1 cut(s) 379
TspRI CASTG 1 cut(s) 739
XapI RAATTY 1 cut(s) 404
XhoI CTCGAG 1 cut(s) 805
XspI CTAG 1 cut(s) 828
ZrmI AGTACT 1 cut(s) 61
Zsp2I ATGCAT 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.