FvH4_5g22130

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012280
Physical Location & Seq
Forward (+)
13455917 .. 13461792
5876 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22130.t1

Sequence Viewer

Length: 1218 bp
ATGGCGTCCGAGATTTTCAATGACAAGAATGCTGTATTCAAGAAGCTGAAATCCAAGTCGGAGAACAAGATGTGCTTCGATTGTAACGCAAAGAACCCAACTTGGGCTTCGGTTACATATGGGATCTTCCTCTGCATCGATTGCTCGGCCGTGCATCGCAGTCTCGGCGTTCACATCAGCTTTGTTAGATCTACGAATTTAGATTCATGGACTCCAGAACAGCTGAGAACAATGACCTTTGGGGGGAATAATCGTGCACAGGTTTTCTTTAAACAACATGGATGGACAGATGGAGGCAAAATTGAGGCCAAATATACATCTCGAGCTGCCGAGTTATATAGGCAGATACTCTCTAAAGAAGTAGCAAAAAGTATGGTAGAAGACGCAGGTCTTCCATCATCACCTGTTGCATCTCAGTCAGGACAAGCATCTAATGGGCTTCCTGATGTCAAGACCAGTGAAGCAATGAAAGAAAAACCCATAGCAAAGCAAGAAACACCTGAAGTTTCTGCTTCCCCTAAAGCCCCCCATGTATTTGTGACCAGTACCGTCAAGAAGCCCCTCGGTGCTAAGAAAATTGGGAAAACTGGGGGACTTGGTGCCCGCAAACTTACTACAAAGCCAAATGAAAGTCTGTATGATCAGAAACCTGAAGAACCAGTTATCCCAGTAGCTTCCTCAACCAAGAGCAACGCACCAGCTGTCACACCCCTGGCTTCTCGATTTGAGTATGTAGAAGTTCTGCAAACAACTGATATGAACTCTAATGGGCATCAGGTAACTGGCCATGTTACTGCACCCAAATCATCAAATTTCTTTGCGGATTTTGGAATGGATAGCAGTTATCCCAAGAGAACTAGTTCAAACTCCTCAAAAGTGCAAATTCAGGAAACTGACGAGGCAAGGAAGAAATTTACAAATGCAAAATCTATTTCTTCCGCACAATTTTTCGGTGATCAGAGCAAATCTGCTGATGTTGAAGCTCACGCCTCATTGCAGAAGTTCTCTGGTTCTGCTGCCATCTCCAGTGCTGACCTTTTCGGTGACAGAGGAGATGATATCCCTCTTGATCTTACTGCGAGTGACCTTATCAACCGTCTATCTTTTCAGGCCCAGCAGGATATCTCTTCTTTAAAAAATATTGCAGGAGAGACCGGGAAGAAGCTTAGCTCCTTGGCATCCAATTTGATGACAGATCTTCAAGACAGAATCCTCTGA

Protein Analysis

406

Amino Acids

43.81

Weight (kDa)

9.01

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 11 - 115 3.7e-36 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17890 AT4G17890
fragaria_vesca FvH4_5g22130
malus_domestica MD00G1122000.v1.1 MD00G1183400.v1.1 MD06G1034600.v1.1
prunus_persica Prupe.5G035300_v2.0.a1
pyrus_communis pycom06g02860 pycom16g24390
rosa_chinensis RchiOBHm_Chr7g0209771
rosa_laevigata RLG00000003094
rosa_multiflora Rmu_sc0001818.1_g000012
rosa_roxburghii Rroxscaffold_3G00248980
rosa_rugosa Rorug07G0116100
rosa_samantha Rh7AG249900 Rh7BG243800 Rh7CG265900 Rh7DG255700
rosa_wichuraiana Rw7G021170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 377
AccB1I GGYRCC 1 cut(s) 599
AciI CCGC 3 cut(s) 604, 821, 939
AclWI GGATC 1 cut(s) 131
AcoI YGGCCR 2 cut(s) 147, 784
AcsI RAATTY 4 cut(s) 196, 811, 882, 911
AcuI CTGAAG 2 cut(s) 522, 672
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 547
AfiI CCNNNNNNNGG 2 cut(s) 103, 243
AgsI TTSAA 5 cut(s) 19, 40, 864, 980, 1202
AhlI ACTAGT 1 cut(s) 857
AjnI CCWGG 1 cut(s) 711
AjuI GAANNNNNNNTTGG 2 cut(s) 91, 123
AloI GAACNNNNNNTCC 2 cut(s) 648, 680
AluBI AGCT 9 cut(s) 46, 180, 223, 326, 674, 701, 983, 1165, 1170
AluI AGCT 9 cut(s) 46, 180, 223, 326, 674, 701, 983, 1165, 1170
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 2 cut(s) 167, 1145
Alw44I GTGCAC 1 cut(s) 255
AlwI GGATC 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 4 cut(s) 147, 306, 784, 1110
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 2 cut(s) 326, 1016
ApoI RAATTY 4 cut(s) 196, 811, 882, 911
Asp700I GAANNNNTTC 1 cut(s) 859
AspS9I GGNCC 1 cut(s) 1111
AsuC2I CCSGG 1 cut(s) 1156
AsuHPI GGTGA 3 cut(s) 393, 965, 1055
AvaI CYCGRG 1 cut(s) 321
BaeGI GKGCMC 2 cut(s) 259, 604
BalI TGGCCA 1 cut(s) 786
BanI GGYRCC 1 cut(s) 599
BbsI GAAGAC 2 cut(s) 383, 387
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 2 cut(s) 313, 1003
BccI CCATC 4 cut(s) 276, 284, 403, 1028
BceAI ACGGC 1 cut(s) 134
BciT130I CCWGG 1 cut(s) 713
BclI TGATCA 2 cut(s) 640, 955
BcnI CCSGG 1 cut(s) 1156
BcoDI GTCTC 2 cut(s) 167, 1145
BcuI ACTAGT 1 cut(s) 857
BfaI CTAG 1 cut(s) 858
BfuAI ACCTGC 1 cut(s) 377
BglII AGATCT 2 cut(s) 188, 1195
BisI GCNGC 2 cut(s) 327, 1017
BlpI GCTNAGC 1 cut(s) 1166
BlsI GCNGC 2 cut(s) 328, 1018
Bme1390I CCNGG 2 cut(s) 713, 1156
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 1 cut(s) 1111
BmiI GGNNCC 1 cut(s) 601
BmrFI CCNGG 2 cut(s) 713, 1156
BmrI ACTGGG 2 cut(s) 597, 662
BmsI GCATC 6 cut(s) 144, 163, 419, 437, 781, 1187
BmuI ACTGGG 2 cut(s) 597, 662
BoxI GACNNNNGTC 1 cut(s) 387
BpiI GAAGAC 2 cut(s) 383, 387
BpmI CTGGAG 2 cut(s) 198, 1009
Bpu1102I GCTNAGC 1 cut(s) 1166
BpuMI CCSGG 1 cut(s) 1156
Bsa29I ATCGAT 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1145
BsaJI CCNNGG 3 cut(s) 562, 711, 1173
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 243
Bse1I ACTGG 7 cut(s) 456, 543, 592, 659, 668, 787, 1026
Bse3DI GCAATG 2 cut(s) 471, 992
BseBI CCWGG 1 cut(s) 713
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 562, 711, 1173
BseGI GGATG 2 cut(s) 287, 1178
BseLI CCNNNNNNNGG 2 cut(s) 103, 243
BseMI GCAATG 2 cut(s) 471, 992
BseMII CTCAG 2 cut(s) 215, 428
BseNI ACTGG 7 cut(s) 456, 543, 592, 659, 668, 787, 1026
BseRI GAGGAG 2 cut(s) 859, 1065
BseSI GKGCMC 2 cut(s) 259, 604
BseX3I CGGCCG 1 cut(s) 147
BseXI GCAGC 2 cut(s) 313, 1003
BseYI CCCAGC 1 cut(s) 1113
BsgI GTGCAG 1 cut(s) 780
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 4 cut(s) 149, 308, 786, 1112
BshNI GGYRCC 1 cut(s) 599
BshVI ATCGAT 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 259
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 1 cut(s) 1155
BslFI GGGAC 1 cut(s) 606
BslI CCNNNNNNNGG 2 cut(s) 103, 243
BsmAI GTCTC 2 cut(s) 167, 1145
BsmFI GGGAC 1 cut(s) 606
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 4 cut(s) 149, 308, 786, 1112
Bso31I GGTCTC 1 cut(s) 1145
BsoBI CYCGRG 1 cut(s) 321
Bsp1286I GDGCHC 2 cut(s) 259, 604
Bsp143I GATC 6 cut(s) 123, 188, 640, 955, 1069, 1195
Bsp1720I GCTNAGC 1 cut(s) 1166
BspACI CCGC 3 cut(s) 604, 821, 939
BspANI GGCC 4 cut(s) 149, 308, 786, 1112
BspCNI CTCAG 2 cut(s) 216, 427
BspDI ATCGAT 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 601
BspMI ACCTGC 1 cut(s) 377
BspPI GGATC 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 599
BspTNI GGTCTC 1 cut(s) 1145
BsrDI GCAATG 2 cut(s) 471, 992
BsrI ACTGG 7 cut(s) 456, 543, 592, 659, 668, 787, 1026
BssECI CCNNGG 3 cut(s) 562, 711, 1173
BssMI GATC 6 cut(s) 123, 188, 640, 955, 1069, 1195
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 1173
Bst2UI CCWGG 1 cut(s) 713
Bst4CI ACNGT 2 cut(s) 550, 1097
Bst6I CTCTTC 1 cut(s) 1132
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 604
BstDEI CTNAG 4 cut(s) 224, 414, 570, 1166
BstF5I GGATG 2 cut(s) 287, 1178
BstKTI GATC 6 cut(s) 126, 191, 643, 958, 1072, 1198
BstMAI GTCTC 2 cut(s) 167, 1145
BstMBI GATC 6 cut(s) 123, 188, 640, 955, 1069, 1195
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 2 cut(s) 141, 165
BstNI CCWGG 1 cut(s) 713
BstPAI GACNNNNGTC 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 711, 1154
BstSLI GKGCMC 2 cut(s) 259, 604
BstV1I GCAGC 2 cut(s) 313, 1003
BstV2I GAAGAC 2 cut(s) 383, 387
BstX2I RGATCY 3 cut(s) 123, 188, 1195
BstYI RGATCY 3 cut(s) 123, 188, 1195
BstZI CGGCCG 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 138
BsuRI GGCC 4 cut(s) 149, 308, 786, 1112
BsuTUI ATCGAT 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 140
BtsCI GGATG 2 cut(s) 287, 1178
BtsIMutI CAGTG 2 cut(s) 463, 1033
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 1 cut(s) 604
Cfr13I GGNCC 1 cut(s) 1111
ClaI ATCGAT 1 cut(s) 138
CseI GACGC 1 cut(s) 392
Csp6I GTAC 1 cut(s) 546
CviAII CATG 4 cut(s) 207, 278, 530, 788
CviQI GTAC 1 cut(s) 546
DdeI CTNAG 4 cut(s) 224, 414, 570, 1166
DpnI GATC 6 cut(s) 125, 190, 642, 957, 1071, 1197
DpnII GATC 6 cut(s) 123, 188, 640, 955, 1069, 1195
DraI TTTAAA 2 cut(s) 271, 1134
EaeI YGGCCR 2 cut(s) 147, 784
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 1132
EarI CTCTTC 1 cut(s) 1132
EclXI CGGCCG 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 1173
Eco31I GGTCTC 1 cut(s) 1145
Eco32I GATATC 2 cut(s) 1060, 1123
Eco52I CGGCCG 1 cut(s) 147
Eco57I CTGAAG 2 cut(s) 522, 672
Eco88I CYCGRG 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 711
EcoRV GATATC 2 cut(s) 1060, 1123
EcoT14I CCWWGG 1 cut(s) 1173
ErhI CCWWGG 1 cut(s) 1173
FaeI CATG 4 cut(s) 210, 281, 533, 791
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FaqI GGGAC 1 cut(s) 606
FatI CATG 4 cut(s) 206, 277, 529, 787
FauI CCCGC 1 cut(s) 611
FauNDI CATATG 1 cut(s) 118
FbaI TGATCA 2 cut(s) 640, 955
Fnu4HI GCNGC 2 cut(s) 327, 1017
FokI GGATG 2 cut(s) 294, 1165
Fsp4HI GCNGC 2 cut(s) 327, 1017
FspBI CTAG 1 cut(s) 858
GluI GCNGC 2 cut(s) 327, 1017
GsaI CCCAGC 1 cut(s) 1117
GsuI CTGGAG 2 cut(s) 198, 1009
HaeIII GGCC 4 cut(s) 149, 308, 786, 1112
HapII CCGG 1 cut(s) 1155
HgaI GACGC 1 cut(s) 392
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 210, 281, 533, 791
HindIII AAGCTT 1 cut(s) 1163
HinfI GANTC 3 cut(s) 203, 211, 1209
HpaII CCGG 1 cut(s) 1155
HphI GGTGA 3 cut(s) 393, 965, 1055
Hpy166II GTNNAC 2 cut(s) 172, 257
Hpy188I TCNGA 5 cut(s) 10, 61, 645, 960, 1217
Hpy8I GTNNAC 2 cut(s) 172, 257
HpyCH4III ACNGT 2 cut(s) 550, 1097
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 165
HpyF3I CTNAG 4 cut(s) 224, 414, 570, 1166
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 210, 281, 533, 791
Ksp22I TGATCA 2 cut(s) 640, 955
Kzo9I GATC 6 cut(s) 123, 188, 640, 955, 1069, 1195
LmnI GCTCC 1 cut(s) 1175
Lsp1109I GCAGC 2 cut(s) 313, 1003
LweI GCATC 6 cut(s) 144, 163, 419, 437, 781, 1187
MaeI CTAG 1 cut(s) 858
MaeIII GTNAC 8 cut(s) 83, 112, 538, 703, 778, 790, 1043, 1082
MalI GATC 6 cut(s) 125, 190, 642, 957, 1071, 1197
MboI GATC 6 cut(s) 123, 188, 640, 955, 1069, 1195
MboII GAAGA 9 cut(s) 118, 383, 392, 665, 919, 927, 1119, 1171, 1190
MflI RGATCY 3 cut(s) 123, 188, 1195
MhlI GDGCHC 2 cut(s) 259, 604
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 8 cut(s) 196, 300, 576, 811, 882, 911, 944, 1183
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 1 cut(s) 205
MmeI TCCRAC 1 cut(s) 39
Mox20I TGGCCA 1 cut(s) 786
MroXI GAANNNNTTC 1 cut(s) 859
MscI TGGCCA 1 cut(s) 786
MseI TTAA 2 cut(s) 270, 1133
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 2 cut(s) 223, 701
MspI CCGG 1 cut(s) 1155
MspR9I CCNGG 2 cut(s) 713, 1156
Mva1269I GAATGC 1 cut(s) 34
MvaI CCWGG 1 cut(s) 713
MwoI GCNNNNNNNGC 2 cut(s) 141, 165
NciI CCSGG 1 cut(s) 1156
NdeI CATATG 1 cut(s) 118
NdeII GATC 6 cut(s) 123, 188, 640, 955, 1069, 1195
NlaIII CATG 4 cut(s) 210, 281, 533, 791
NlaIV GGNNCC 1 cut(s) 601
NmeAIII GCCGAG 3 cut(s) 125, 144, 355
NmuCI GTSAC 4 cut(s) 538, 703, 1043, 1082
PaeR7I CTCGAG 1 cut(s) 321
PctI GAATGC 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 859
PfeI GAWTC 2 cut(s) 203, 1209
PkrI GCNGC 2 cut(s) 328, 1018
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PshAI GACNNNNGTC 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 711
PspFI CCCAGC 1 cut(s) 1113
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 1 cut(s) 601
PspPI GGNCC 1 cut(s) 1111
PsuI RGATCY 3 cut(s) 123, 188, 1195
PvuII CAGCTG 2 cut(s) 223, 701
RsaI GTAC 1 cut(s) 547
RsaNI GTAC 1 cut(s) 546
SaqAI TTAA 2 cut(s) 270, 1133
SatI GCNGC 2 cut(s) 327, 1017
Sau3AI GATC 6 cut(s) 123, 188, 640, 955, 1069, 1195
Sau96I GGNCC 1 cut(s) 1111
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 2 cut(s) 713, 1156
SduI GDGCHC 2 cut(s) 259, 604
SfaNI GCATC 6 cut(s) 144, 163, 419, 437, 781, 1187
Sfr274I CTCGAG 1 cut(s) 321
SlaI CTCGAG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
SpeI ACTAGT 1 cut(s) 857
Sse9I AATT 8 cut(s) 196, 300, 576, 811, 882, 911, 944, 1183
SsiI CCGC 3 cut(s) 604, 821, 939
SspI AATATT 1 cut(s) 1141
SspMI CTAG 1 cut(s) 858
StyD4I CCNGG 2 cut(s) 711, 1154
StyI CCWWGG 1 cut(s) 1173
TaaI ACNGT 2 cut(s) 550, 1097
TaqI TCGA 4 cut(s) 78, 138, 322, 721
TasI AATT 8 cut(s) 196, 300, 576, 811, 882, 911, 944, 1183
TfiI GAWTC 2 cut(s) 203, 1209
Tru1I TTAA 2 cut(s) 270, 1133
Tru9I TTAA 2 cut(s) 270, 1133
TscAI CASTG 2 cut(s) 463, 1033
TseFI GTSAC 4 cut(s) 538, 703, 1043, 1082
TseI GCWGC 2 cut(s) 326, 1016
Tsp45I GTSAC 4 cut(s) 538, 703, 1043, 1082
TspDTI ATGAA 4 cut(s) 195, 482, 642, 773
TspRI CASTG 2 cut(s) 463, 1033
VneI GTGCAC 1 cut(s) 255
XapI RAATTY 4 cut(s) 196, 811, 882, 911
XhoI CTCGAG 1 cut(s) 321
XmnI GAANNNNTTC 1 cut(s) 859
XspI CTAG 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.