Rroxscaffold_3G00248980

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012280
Physical Location & Seq
Reverse (-)
42476498 .. 42481806
5309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00248980.1

Sequence Viewer

Length: 1209 bp
ATGGCGTCCGAGATTTTCAATGACAAGAATGCTGTTTTCAAAAAGCTGAAATCGAAGTCGGAGAACAAGATGTGCTTCGATTGCAACGCGAAGAACCCAACTTGGGCTTCAGTTACATATGGGATCTTTCTCTGCATCGATTGCTCGGCCGTCCATCGCAGTCTCGGCGTTCACATCAGCTTTGTTAGATCTACGAATTTAGACTCCTGGACTCCTGAACAGCTGAGAACAATGACCTTTGGGGGAAATAATCGCGCACAGGTTTTCTTTAAACAGCATGGATGGACAGATGGAGGCAAAATCGAGGCCAAATATACATCACGAGCTGCCGAGTTATATAGGCAGATACTTTCTAAAGAAGTAGCAAAAAGTATGGCAGAAGATGCAGGTCTTCCACCATCACCTGTTGCATCTCAGTCGGGACAAGCATCTAATGGGCTTCCTGATATCAAGACCAGTGAAGCAACAAAAGAAAAGCCTATAGCAAGGCAAGAAACACCTGAAGTTTCTGATTCCCCCAAAGCCCCCCATGTGTTTGTTACCAGTACCGTCAAGAAGCCCCTTGGTGCTAAGAAAACTGGGAAAACTGGGGGACTTGGTGCTCGCAAACTCACTAAAAAGCCAAATGAAAGTCTGTATGATCAGAAGCCTGAAGAACCAGTTATCCCAGTAGTTTCCTCAACCAAAAGCACTCCACCAGCTGTCACACCCCTTGCTTCTCGATTTGAGTATGTAGAAGCTCTTCAAACTACTGAGATAAATTCTAATGGCGCTACTGGCCATGTTTCTGCACCCAAGTCATCGAGTTTCTTTGCGGATTTCGGAATGGATAGCAGTTATCCCAAGAGAACTAGTTCAAACTCCTCAAAAGTGCAAATTCAGGAAACTGATGAGGCAAGGAAGAAATTCACAAATGCAAAATCTATTTCTTCCGCACAATTTTTTGGTGATCAGAGCAAATCTGCTGACGTTGATGCCCAAGCCTCCTTGCAGAAGTTTTCTGGTTCTGCTGCCATCTCCAGTGCTGACCTTTTCGGTGACAGAGAAGATATCCCTCTTGATCTTACTGCGGGTGACCTTATTAACCGTCTGTCTTTTCAGGCACAGCAGGATATCTCCTCTTTAAAAAATATTGCAGGAGAGACCGGGAAGAAGCTTAGCTCCTTGGCATCCAATTTAATGACGGATCTTCAGGACAGAATCCTCTGA

Protein Analysis

402

Amino Acids

43.44

Weight (kDa)

9.01

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 11 - 115 3.6e-36 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17890 AT4G17890
fragaria_vesca FvH4_5g22130
malus_domestica MD00G1122000.v1.1 MD00G1183400.v1.1 MD06G1034600.v1.1
prunus_persica Prupe.5G035300_v2.0.a1
pyrus_communis pycom06g02860 pycom16g24390
rosa_chinensis RchiOBHm_Chr7g0209771
rosa_laevigata RLG00000003094
rosa_multiflora Rmu_sc0001818.1_g000012
rosa_roxburghii Rroxscaffold_3G00248980
rosa_rugosa Rorug07G0116100
rosa_samantha Rh7AG249900 Rh7BG243800 Rh7CG265900 Rh7DG255700
rosa_wichuraiana Rw7G021170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 377
AccII CGCG 2 cut(s) 89, 255
AciI CCGC 3 cut(s) 815, 933, 1070
AclWI GGATC 2 cut(s) 131, 1194
AcoI YGGCCR 2 cut(s) 147, 778
AcsI RAATTY 4 cut(s) 196, 760, 876, 905
AcuI CTGAAG 4 cut(s) 93, 522, 672, 1175
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 547
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 4 cut(s) 19, 40, 746, 858
AhlI ACTAGT 1 cut(s) 851
AjnI CCWGG 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 91, 123
AloI GAACNNNNNNTCC 2 cut(s) 648, 680
AluBI AGCT 8 cut(s) 46, 180, 223, 326, 701, 740, 1156, 1161
AluI AGCT 8 cut(s) 46, 180, 223, 326, 701, 740, 1156, 1161
Alw21I GWGCWC 1 cut(s) 604
Alw26I GTCTC 2 cut(s) 167, 1136
AlwI GGATC 2 cut(s) 131, 1194
AoxI GGCC 3 cut(s) 147, 306, 778
ApeKI GCWGC 2 cut(s) 326, 1010
ApoI RAATTY 4 cut(s) 196, 760, 876, 905
Asp700I GAANNNNTTC 3 cut(s) 741, 853, 905
AspLEI GCGC 2 cut(s) 257, 773
AsuC2I CCSGG 1 cut(s) 1147
AsuHPI GGTGA 4 cut(s) 393, 959, 1049, 1085
BalI TGGCCA 1 cut(s) 780
BarI GAAGNNNNNNTAC 2 cut(s) 726, 758
BauI CACGAG 1 cut(s) 321
BbsI GAAGAC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 604
BbvI GCAGC 2 cut(s) 313, 997
BccI CCATC 5 cut(s) 162, 276, 284, 406, 1022
BceAI ACGGC 1 cut(s) 134
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BciT130I CCWGG 1 cut(s) 208
BclI TGATCA 2 cut(s) 640, 949
BcnI CCSGG 1 cut(s) 1147
BcoDI GTCTC 2 cut(s) 167, 1136
BcuI ACTAGT 1 cut(s) 851
BfaI CTAG 1 cut(s) 852
BfmI CTRYAG 1 cut(s) 480
BfoI RGCGCY 1 cut(s) 774
BfuAI ACCTGC 1 cut(s) 377
BglII AGATCT 1 cut(s) 188
BisI GCNGC 2 cut(s) 327, 1011
BlpI GCTNAGC 1 cut(s) 1157
BlsI GCNGC 2 cut(s) 328, 1012
Bme1390I CCNGG 2 cut(s) 208, 1147
BmrFI CCNGG 2 cut(s) 208, 1147
BmrI ACTGGG 3 cut(s) 588, 597, 662
BmsI GCATC 6 cut(s) 144, 373, 419, 437, 964, 1178
BmuI ACTGGG 3 cut(s) 588, 597, 662
BpiI GAAGAC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 1003
Bpu1102I GCTNAGC 1 cut(s) 1157
BpuMI CCSGG 1 cut(s) 1147
Bsa29I ATCGAT 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1136
BsaJI CCNNGG 2 cut(s) 562, 1164
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 8 cut(s) 456, 543, 583, 592, 659, 668, 781, 1020
BseBI CCWGG 1 cut(s) 208
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 562, 1164
BseGI GGATG 2 cut(s) 287, 1169
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 3 cut(s) 215, 428, 744
BseNI ACTGG 8 cut(s) 456, 543, 583, 592, 659, 668, 781, 1020
BseRI GAGGAG 2 cut(s) 853, 1108
BseX3I CGGCCG 1 cut(s) 147
BseXI GCAGC 2 cut(s) 313, 997
BsgI GTGCAG 1 cut(s) 774
Bsh1236I CGCG 2 cut(s) 89, 255
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 3 cut(s) 149, 308, 780
BshVI ATCGAT 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 604
BsiSI CCGG 1 cut(s) 1146
BslFI GGGAC 2 cut(s) 435, 606
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 2 cut(s) 167, 1136
BsmFI GGGAC 2 cut(s) 435, 606
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 3 cut(s) 149, 308, 780
Bso31I GGTCTC 1 cut(s) 1136
Bsp1286I GDGCHC 1 cut(s) 604
Bsp143I GATC 6 cut(s) 123, 188, 640, 949, 1060, 1186
Bsp1720I GCTNAGC 1 cut(s) 1157
BspACI CCGC 3 cut(s) 815, 933, 1070
BspANI GGCC 3 cut(s) 149, 308, 780
BspCNI CTCAG 3 cut(s) 216, 427, 745
BspDI ATCGAT 1 cut(s) 138
BspFNI CGCG 2 cut(s) 89, 255
BspMI ACCTGC 1 cut(s) 377
BspPI GGATC 2 cut(s) 131, 1194
BspQI GCTCTTC 1 cut(s) 747
BspTNI GGTCTC 1 cut(s) 1136
BsrI ACTGG 8 cut(s) 456, 543, 583, 592, 659, 668, 781, 1020
BssECI CCNNGG 2 cut(s) 562, 1164
BssMI GATC 6 cut(s) 123, 188, 640, 949, 1060, 1186
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 321
BssT1I CCWWGG 2 cut(s) 562, 1164
Bst2BI CACGAG 1 cut(s) 321
Bst2UI CCWGG 1 cut(s) 208
Bst4CI ACNGT 2 cut(s) 550, 1088
Bst6I CTCTTC 1 cut(s) 747
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 141, 383
BstC8I GCNNGC 1 cut(s) 604
BstDEI CTNAG 5 cut(s) 224, 414, 570, 753, 1157
BstEII GGTNACC 1 cut(s) 1073
BstF5I GGATG 2 cut(s) 287, 1169
BstFNI CGCG 2 cut(s) 89, 255
BstH2I RGCGCY 1 cut(s) 774
BstHHI GCGC 2 cut(s) 257, 773
BstKTI GATC 6 cut(s) 126, 191, 643, 952, 1063, 1189
BstMAI GTCTC 2 cut(s) 167, 1136
BstMBI GATC 6 cut(s) 123, 188, 640, 949, 1060, 1186
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 5 cut(s) 81, 141, 165, 383, 777
BstNI CCWGG 1 cut(s) 208
BstPI GGTNACC 1 cut(s) 1073
BstSCI CCNGG 2 cut(s) 206, 1145
BstSFI CTRYAG 1 cut(s) 480
BstUI CGCG 2 cut(s) 89, 255
BstV1I GCAGC 2 cut(s) 313, 997
BstV2I GAAGAC 1 cut(s) 383
BstX2I RGATCY 3 cut(s) 123, 188, 1186
BstYI RGATCY 3 cut(s) 123, 188, 1186
BstZI CGGCCG 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 138
BsuRI GGCC 3 cut(s) 149, 308, 780
BsuTUI ATCGAT 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 140
BtsCI GGATG 2 cut(s) 287, 1169
BtsIMutI CAGTG 2 cut(s) 463, 1027
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 1 cut(s) 604
CfoI GCGC 2 cut(s) 257, 773
ClaI ATCGAT 1 cut(s) 138
Csp6I GTAC 1 cut(s) 546
CviAII CATG 3 cut(s) 278, 530, 782
CviQI GTAC 1 cut(s) 546
DdeI CTNAG 5 cut(s) 224, 414, 570, 753, 1157
DpnI GATC 6 cut(s) 125, 190, 642, 951, 1062, 1188
DpnII GATC 6 cut(s) 123, 188, 640, 949, 1060, 1186
DraI TTTAAA 2 cut(s) 271, 1125
EaeI YGGCCR 2 cut(s) 147, 778
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 747
EarI CTCTTC 1 cut(s) 747
EclXI CGGCCG 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 562, 1164
Eco31I GGTCTC 1 cut(s) 1136
Eco32I GATATC 3 cut(s) 448, 1051, 1114
Eco52I CGGCCG 1 cut(s) 147
Eco57I CTGAAG 4 cut(s) 93, 522, 672, 1175
Eco91I GGTNACC 1 cut(s) 1073
EcoO65I GGTNACC 1 cut(s) 1073
EcoRII CCWGG 1 cut(s) 206
EcoRV GATATC 3 cut(s) 448, 1051, 1114
EcoT14I CCWWGG 2 cut(s) 562, 1164
ErhI CCWWGG 2 cut(s) 562, 1164
FaeI CATG 3 cut(s) 281, 533, 785
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FaqI GGGAC 2 cut(s) 435, 606
FatI CATG 3 cut(s) 277, 529, 781
FauI CCCGC 1 cut(s) 1063
FauNDI CATATG 1 cut(s) 118
FbaI TGATCA 2 cut(s) 640, 949
Fnu4HI GCNGC 2 cut(s) 327, 1011
FokI GGATG 2 cut(s) 294, 1156
Fsp4HI GCNGC 2 cut(s) 327, 1011
FspBI CTAG 1 cut(s) 852
GlaI GCGC 2 cut(s) 256, 772
GluI GCNGC 2 cut(s) 327, 1011
GsuI CTGGAG 1 cut(s) 1003
HaeII RGCGCY 1 cut(s) 774
HaeIII GGCC 3 cut(s) 149, 308, 780
HapII CCGG 1 cut(s) 1146
HhaI GCGC 2 cut(s) 257, 773
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 281, 533, 785
Hin6I GCGC 2 cut(s) 255, 771
HinP1I GCGC 2 cut(s) 255, 771
HindIII AAGCTT 1 cut(s) 1154
HinfI GANTC 4 cut(s) 203, 211, 512, 1200
HpaII CCGG 1 cut(s) 1146
HphI GGTGA 4 cut(s) 393, 959, 1049, 1085
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 7 cut(s) 10, 61, 511, 645, 824, 954, 1208
Hpy8I GTNNAC 1 cut(s) 172
HpyCH4III ACNGT 2 cut(s) 550, 1088
HpyCH4IV ACGT 1 cut(s) 969
HpyCH4V TGCA 9 cut(s) 84, 135, 386, 410, 791, 874, 917, 991, 1136
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 141, 165, 383, 777
HpyF3I CTNAG 5 cut(s) 224, 414, 570, 753, 1157
HpySE526I ACGT 1 cut(s) 969
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 281, 533, 785
HspAI GCGC 2 cut(s) 255, 771
Ksp22I TGATCA 2 cut(s) 640, 949
Kzo9I GATC 6 cut(s) 123, 188, 640, 949, 1060, 1186
LguI GCTCTTC 1 cut(s) 747
LmnI GCTCC 1 cut(s) 1166
Lsp1109I GCAGC 2 cut(s) 313, 997
LweI GCATC 6 cut(s) 144, 373, 419, 437, 964, 1178
MaeI CTAG 1 cut(s) 852
MaeII ACGT 1 cut(s) 969
MaeIII GTNAC 5 cut(s) 112, 538, 703, 1037, 1073
MalI GATC 6 cut(s) 125, 190, 642, 951, 1062, 1188
MboI GATC 6 cut(s) 123, 188, 640, 949, 1060, 1186
MflI RGATCY 3 cut(s) 123, 188, 1186
MhlI GDGCHC 1 cut(s) 604
MlsI TGGCCA 1 cut(s) 780
MluCI AATT 6 cut(s) 196, 760, 876, 905, 938, 1174
MluNI TGGCCA 1 cut(s) 780
MlyI GAGTC 2 cut(s) 197, 205
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 8 cut(s) 287, 298, 688, 874, 886, 994, 1065, 1129
Mox20I TGGCCA 1 cut(s) 780
MroXI GAANNNNTTC 3 cut(s) 741, 853, 905
MscI TGGCCA 1 cut(s) 780
MseI TTAA 4 cut(s) 270, 1083, 1124, 1178
Msp20I TGGCCA 1 cut(s) 780
MspA1I CMGCKG 2 cut(s) 223, 701
MspI CCGG 1 cut(s) 1146
MspR9I CCNGG 2 cut(s) 208, 1147
Mva1269I GAATGC 1 cut(s) 34
MvaI CCWGG 1 cut(s) 208
MvnI CGCG 2 cut(s) 89, 255
MwoI GCNNNNNNNGC 5 cut(s) 81, 141, 165, 383, 777
NciI CCSGG 1 cut(s) 1147
NdeI CATATG 1 cut(s) 118
NdeII GATC 6 cut(s) 123, 188, 640, 949, 1060, 1186
NlaIII CATG 3 cut(s) 281, 533, 785
NmeAIII GCCGAG 3 cut(s) 125, 144, 355
NmuCI GTSAC 3 cut(s) 703, 1037, 1073
PciSI GCTCTTC 1 cut(s) 747
PctI GAATGC 1 cut(s) 34
PdmI GAANNNNTTC 3 cut(s) 741, 853, 905
PfeI GAWTC 2 cut(s) 512, 1200
PfoI TCCNGGA 1 cut(s) 206
PkrI GCNGC 2 cut(s) 328, 1012
PleI GAGTC 2 cut(s) 197, 205
PpsI GAGTC 2 cut(s) 197, 205
Psp6I CCWGG 1 cut(s) 206
PspEI GGTNACC 1 cut(s) 1073
PspGI CCWGG 1 cut(s) 206
PsuI RGATCY 3 cut(s) 123, 188, 1186
PvuII CAGCTG 2 cut(s) 223, 701
RsaI GTAC 1 cut(s) 547
RsaNI GTAC 1 cut(s) 546
SapI GCTCTTC 1 cut(s) 747
SaqAI TTAA 4 cut(s) 270, 1083, 1124, 1178
SatI GCNGC 2 cut(s) 327, 1011
Sau3AI GATC 6 cut(s) 123, 188, 640, 949, 1060, 1186
SchI GAGTC 2 cut(s) 197, 205
ScrFI CCNGG 2 cut(s) 208, 1147
SduI GDGCHC 1 cut(s) 604
SfaNI GCATC 6 cut(s) 144, 373, 419, 437, 964, 1178
SfcI CTRYAG 1 cut(s) 480
SpeI ACTAGT 1 cut(s) 851
Sse9I AATT 6 cut(s) 196, 760, 876, 905, 938, 1174
SsiI CCGC 3 cut(s) 815, 933, 1070
SspI AATATT 1 cut(s) 1132
SspMI CTAG 1 cut(s) 852
StyD4I CCNGG 2 cut(s) 206, 1145
StyI CCWWGG 2 cut(s) 562, 1164
TaaI ACNGT 2 cut(s) 550, 1088
TaiI ACGT 1 cut(s) 972
TaqI TCGA 6 cut(s) 53, 78, 138, 303, 721, 803
TasI AATT 6 cut(s) 196, 760, 876, 905, 938, 1174
TfiI GAWTC 2 cut(s) 512, 1200
Tru1I TTAA 4 cut(s) 270, 1083, 1124, 1178
Tru9I TTAA 4 cut(s) 270, 1083, 1124, 1178
TscAI CASTG 2 cut(s) 463, 1027
TseFI GTSAC 3 cut(s) 703, 1037, 1073
TseI GCWGC 2 cut(s) 326, 1010
Tsp45I GTSAC 3 cut(s) 703, 1037, 1073
TspDTI ATGAA 1 cut(s) 642
TspGWI ACGGA 1 cut(s) 1199
TspRI CASTG 2 cut(s) 463, 1027
XapI RAATTY 4 cut(s) 196, 760, 876, 905
XmnI GAANNNNTTC 3 cut(s) 741, 853, 905
XspI CTAG 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.