Rh7AG249900

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0012280
Physical Location & Seq
Forward (+)
24789694 .. 24795822
6129 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG249900.1

Sequence Viewer

Length: 1209 bp
ATGGACGTAAACAGAGACAATTCGACAACAACAAACCACCAACATCGAAAACCTGAGGGGCATTTCCGTCCTTCCCCACAACCCTCTGCGTCCTTCCCCCTGGTAATAAAATGCCCCCGTTTGAAAATCCCAAACCCAAAAAGAAATCAAAAAATCAATTTTGGGGGATTCAAATTGTTCTTTTTTTTTTTTTTTGGTCATAACTTAAAAGAACATCTCGATCTCCTTCTCCTTCTTCTCGATTCAAAGCCTGAGGAGGTGTTCAAGGCACCTCTTCGGATTCAATTTGCCGTCTTTACAATCAAATTAGAAATGGCGTCCGAGATTTTCAATGACAAGAATGCTGTTTTCAAAAAGCTGAAATCGAAGTCGGAGAACAAGATGTGCTTCGATTGTAACGCGAAGAACCCAACTTGGGCTTCAGTTACATACGGGATCTTTCTCTGCATCGATTGCTCGGCCGTCCATCGCAGTCTCGGCGTTCACATCAGCTTTGTTAGATCTACGAATTTAGACTCCTGGACTCCTGAACAGCTGAGAACAATGACCTTTGGGGGGAATAATCGCGCACAGGTTTTCTTTAAACAGCATGGATGGACAGATGGAGGCAAAATTGAGGCCAAATATACATCACGAGCTGCCGAGTTATATAGGCAGATACTTTCTAAAGAAGTAGCAAAAAGTATGGCAGAAGACGCAGGTCTTCCACCATCACCTGTTGCATCTCAGTCGGGACAAGCATCTAATGGGCTTCCTGATATCAAGACCAGTGAAGCAATAAAAGAAAAGCCTATAGCAAGGCAAGAAACACCTGAAGTTTCTGATTCCCCCAAAGCCCCCCATGTATTTGTTACCAGTACCGTCAAGAAGCCCCTTGGTGCTAAGAAAAGTGGGAAAACTGGGGGACTTGGTGCTCGCAAACTCACTACAAAGCCAAATGAAAGTCTGTATGATCAGAAGCCTGAAGAACCAGTTATCCCAGTAGTTTCCTCAACCAAAAGCACTCCACCAGCTGTCACACCCCTTGCTTCTCGATTTGAGTATGTAGAAGCTCTTCAAACTACTGATATAAATTCTAATGGCGCTAGTGTAACTGGCCATGTTTCTGCACCCAAGTCATCGAGTTTCTTTGCGGATTTCGGAATGGATAGCAGTTATCCCAAGAGAACTAGTTCAAACTCCTCAAAAGTGCAAGTAAGTTCTGTTTGA

Protein Analysis

402

Amino Acids

44.32

Weight (kDa)

9.57

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 115 - 219 3.6e-36 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17890 AT4G17890
fragaria_vesca FvH4_5g22130
malus_domestica MD00G1122000.v1.1 MD00G1183400.v1.1 MD06G1034600.v1.1
prunus_persica Prupe.5G035300_v2.0.a1
pyrus_communis pycom06g02860 pycom16g24390
rosa_chinensis RchiOBHm_Chr7g0209771
rosa_laevigata RLG00000003094
rosa_multiflora Rmu_sc0001818.1_g000012
rosa_roxburghii Rroxscaffold_3G00248980
rosa_rugosa Rorug07G0116100
rosa_samantha Rh7AG249900 Rh7BG243800 Rh7CG265900 Rh7DG255700
rosa_wichuraiana Rw7G021170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 689
AccB1I GGYRCC 1 cut(s) 268
AccII CGCG 2 cut(s) 401, 567
AciI CCGC 1 cut(s) 1133
AclWI GGATC 1 cut(s) 443
AcoI YGGCCR 2 cut(s) 459, 1096
AcsI RAATTY 2 cut(s) 508, 1072
AcuI CTGAAG 3 cut(s) 405, 834, 984
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 1 cut(s) 859
AfiI CCNNNNNNNGG 2 cut(s) 415, 555
AgsI TTSAA 9 cut(s) 124, 172, 246, 265, 284, 331, 352, 1058, 1176
AhlI ACTAGT 1 cut(s) 1169
AjnI CCWGG 2 cut(s) 99, 518
AjuI GAANNNNNNNTTGG 2 cut(s) 403, 435
AloI GAACNNNNNNTCC 2 cut(s) 960, 992
AluBI AGCT 6 cut(s) 358, 492, 535, 638, 1013, 1052
AluI AGCT 6 cut(s) 358, 492, 535, 638, 1013, 1052
Alw21I GWGCWC 1 cut(s) 916
Alw26I GTCTC 2 cut(s) 9, 479
AlwI GGATC 1 cut(s) 443
AoxI GGCC 3 cut(s) 459, 618, 1096
ApeKI GCWGC 1 cut(s) 638
ApoI RAATTY 2 cut(s) 508, 1072
Asp700I GAANNNNTTC 2 cut(s) 1053, 1171
AspLEI GCGC 2 cut(s) 569, 1085
AsuHPI GGTGA 1 cut(s) 705
AxyI CCTNAGG 2 cut(s) 54, 252
BalI TGGCCA 1 cut(s) 1098
BanI GGYRCC 1 cut(s) 268
BarI GAAGNNNNNNTAC 2 cut(s) 1038, 1070
BauI CACGAG 1 cut(s) 633
BbsI GAAGAC 2 cut(s) 695, 699
Bbv12I GWGCWC 1 cut(s) 916
BbvI GCAGC 1 cut(s) 625
BccI CCATC 4 cut(s) 474, 588, 596, 718
BceAI ACGGC 2 cut(s) 275, 446
BcgI CGANNNNNNTGC 2 cut(s) 711, 745
BciT130I CCWGG 2 cut(s) 101, 520
BclI TGATCA 1 cut(s) 952
BcoDI GTCTC 2 cut(s) 9, 479
BcuI ACTAGT 1 cut(s) 1169
BfaI CTAG 2 cut(s) 1086, 1170
BfmI CTRYAG 1 cut(s) 792
BfoI RGCGCY 1 cut(s) 1086
BfuAI ACCTGC 1 cut(s) 689
BglII AGATCT 1 cut(s) 500
BisI GCNGC 1 cut(s) 639
BlsI GCNGC 1 cut(s) 640
Bme1390I CCNGG 2 cut(s) 101, 520
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 2 cut(s) 101, 520
BmrI ACTGGG 2 cut(s) 909, 974
BmsI GCATC 3 cut(s) 456, 731, 749
BmuI ACTGGG 2 cut(s) 909, 974
BoxI GACNNNNGTC 1 cut(s) 699
BpiI GAAGAC 2 cut(s) 695, 699
Bsa29I ATCGAT 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 99, 874
Bsc4I CCNNNNNNNGG 2 cut(s) 415, 555
Bse1I ACTGG 6 cut(s) 768, 855, 904, 971, 980, 1099
Bse21I CCTNAGG 2 cut(s) 54, 252
BseBI CCWGG 2 cut(s) 101, 520
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 2 cut(s) 99, 874
BseGI GGATG 1 cut(s) 599
BseLI CCNNNNNNNGG 2 cut(s) 415, 555
BseMII CTCAG 4 cut(s) 45, 243, 527, 740
BseNI ACTGG 6 cut(s) 768, 855, 904, 971, 980, 1099
BseRI GAGGAG 2 cut(s) 269, 1171
BseX3I CGGCCG 1 cut(s) 459
BseXI GCAGC 1 cut(s) 625
BsgI GTGCAG 1 cut(s) 1092
Bsh1236I CGCG 2 cut(s) 401, 567
Bsh1285I CGRYCG 1 cut(s) 462
BshFI GGCC 3 cut(s) 461, 620, 1098
BshNI GGYRCC 1 cut(s) 268
BshVI ATCGAT 1 cut(s) 450
BsiEI CGRYCG 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 916
BslFI GGGAC 2 cut(s) 747, 918
BslI CCNNNNNNNGG 2 cut(s) 415, 555
BsmAI GTCTC 2 cut(s) 9, 479
BsmFI GGGAC 2 cut(s) 747, 918
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 3 cut(s) 461, 620, 1098
Bsp1286I GDGCHC 1 cut(s) 916
Bsp143I GATC 4 cut(s) 220, 435, 500, 952
BspACI CCGC 1 cut(s) 1133
BspANI GGCC 3 cut(s) 461, 620, 1098
BspCNI CTCAG 4 cut(s) 46, 244, 528, 739
BspDI ATCGAT 1 cut(s) 450
BspFNI CGCG 2 cut(s) 401, 567
BspLI GGNNCC 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 689
BspPI GGATC 1 cut(s) 443
BspQI GCTCTTC 1 cut(s) 1059
BspT107I GGYRCC 1 cut(s) 268
BsrI ACTGG 6 cut(s) 768, 855, 904, 971, 980, 1099
BssECI CCNNGG 2 cut(s) 99, 874
BssMI GATC 4 cut(s) 220, 435, 500, 952
BssNI GRCGYC 1 cut(s) 317
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 1 cut(s) 874
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 2 cut(s) 101, 520
Bst4CI ACNGT 1 cut(s) 862
Bst6I CTCTTC 2 cut(s) 279, 1059
BstACI GRCGYC 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 453
BstC8I GCNNGC 1 cut(s) 916
BstDEI CTNAG 5 cut(s) 54, 252, 536, 726, 882
BstF5I GGATG 1 cut(s) 599
BstFNI CGCG 2 cut(s) 401, 567
BstH2I RGCGCY 1 cut(s) 1086
BstHHI GCGC 2 cut(s) 569, 1085
BstKTI GATC 4 cut(s) 223, 438, 503, 955
BstMAI GTCTC 2 cut(s) 9, 479
BstMBI GATC 4 cut(s) 220, 435, 500, 952
BstMCI CGRYCG 1 cut(s) 462
BstMWI GCNNNNNNNGC 3 cut(s) 453, 477, 695
BstNI CCWGG 2 cut(s) 101, 520
BstPAI GACNNNNGTC 1 cut(s) 699
BstSCI CCNGG 2 cut(s) 99, 518
BstSFI CTRYAG 1 cut(s) 792
BstUI CGCG 2 cut(s) 401, 567
BstV1I GCAGC 1 cut(s) 625
BstV2I GAAGAC 2 cut(s) 695, 699
BstX2I RGATCY 2 cut(s) 435, 500
BstYI RGATCY 2 cut(s) 435, 500
BstZI CGGCCG 1 cut(s) 459
Bsu15I ATCGAT 1 cut(s) 450
Bsu36I CCTNAGG 2 cut(s) 54, 252
BsuRI GGCC 3 cut(s) 461, 620, 1098
BsuTUI ATCGAT 1 cut(s) 450
BtgZI GCGATG 1 cut(s) 452
BtsCI GGATG 1 cut(s) 599
BtsIMutI CAGTG 1 cut(s) 775
BveI ACCTGC 1 cut(s) 689
Cac8I GCNNGC 1 cut(s) 916
CfoI GCGC 2 cut(s) 569, 1085
ClaI ATCGAT 1 cut(s) 450
CseI GACGC 3 cut(s) 78, 306, 704
Csp6I GTAC 1 cut(s) 858
CviAII CATG 3 cut(s) 590, 842, 1100
CviQI GTAC 1 cut(s) 858
DdeI CTNAG 5 cut(s) 54, 252, 536, 726, 882
DpnI GATC 4 cut(s) 222, 437, 502, 954
DpnII GATC 4 cut(s) 220, 435, 500, 952
DraI TTTAAA 1 cut(s) 583
EaeI YGGCCR 2 cut(s) 459, 1096
EagI CGGCCG 1 cut(s) 459
Eam1104I CTCTTC 2 cut(s) 279, 1059
EarI CTCTTC 2 cut(s) 279, 1059
EclXI CGGCCG 1 cut(s) 459
Eco130I CCWWGG 1 cut(s) 874
Eco32I GATATC 1 cut(s) 760
Eco52I CGGCCG 1 cut(s) 459
Eco57I CTGAAG 3 cut(s) 405, 834, 984
Eco81I CCTNAGG 2 cut(s) 54, 252
EcoRII CCWGG 2 cut(s) 99, 518
EcoRV GATATC 1 cut(s) 760
EcoT14I CCWWGG 1 cut(s) 874
ErhI CCWWGG 1 cut(s) 874
FaeI CATG 3 cut(s) 593, 845, 1103
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FaqI GGGAC 2 cut(s) 747, 918
FatI CATG 3 cut(s) 589, 841, 1099
FbaI TGATCA 1 cut(s) 952
Fnu4HI GCNGC 1 cut(s) 639
FokI GGATG 1 cut(s) 606
Fsp4HI GCNGC 1 cut(s) 639
FspBI CTAG 2 cut(s) 1086, 1170
GlaI GCGC 2 cut(s) 568, 1084
GluI GCNGC 1 cut(s) 639
HaeII RGCGCY 1 cut(s) 1086
HaeIII GGCC 3 cut(s) 461, 620, 1098
HgaI GACGC 3 cut(s) 78, 306, 704
HhaI GCGC 2 cut(s) 569, 1085
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 3 cut(s) 593, 845, 1103
Hin6I GCGC 2 cut(s) 567, 1083
HinP1I GCGC 2 cut(s) 567, 1083
HinfI GANTC 6 cut(s) 168, 242, 280, 515, 523, 824
HphI GGTGA 1 cut(s) 705
Hpy166II GTNNAC 2 cut(s) 10, 484
Hpy188I TCNGA 6 cut(s) 279, 322, 373, 823, 957, 1142
Hpy188III TCNNGA 9 cut(s) 218, 239, 527, 633, 732, 755, 763, 865, 1032
Hpy8I GTNNAC 2 cut(s) 10, 484
HpyAV CCTTC 4 cut(s) 81, 103, 236, 242
HpyCH4III ACNGT 1 cut(s) 862
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 4 cut(s) 447, 722, 1109, 1192
HpyF10VI GCNNNNNNNGC 3 cut(s) 453, 477, 695
HpyF3I CTNAG 5 cut(s) 54, 252, 536, 726, 882
HpySE526I ACGT 1 cut(s) 6
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 3 cut(s) 593, 845, 1103
HspAI GCGC 2 cut(s) 567, 1083
Ksp22I TGATCA 1 cut(s) 952
Kzo9I GATC 4 cut(s) 220, 435, 500, 952
LguI GCTCTTC 1 cut(s) 1059
Lsp1109I GCAGC 1 cut(s) 625
LweI GCATC 3 cut(s) 456, 731, 749
MaeI CTAG 2 cut(s) 1086, 1170
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 5 cut(s) 395, 424, 850, 1015, 1090
MalI GATC 4 cut(s) 222, 437, 502, 954
MboI GATC 4 cut(s) 220, 435, 500, 952
MboII GAAGA 7 cut(s) 227, 266, 415, 695, 704, 977, 1046
MflI RGATCY 2 cut(s) 435, 500
MhlI GDGCHC 1 cut(s) 916
MlsI TGGCCA 1 cut(s) 1098
MluCI AATT 8 cut(s) 19, 157, 173, 284, 305, 508, 612, 1072
MluNI TGGCCA 1 cut(s) 1098
MlyI GAGTC 2 cut(s) 509, 517
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 9 cut(s) 49, 94, 247, 250, 282, 599, 610, 1000, 1192
Mox20I TGGCCA 1 cut(s) 1098
MroXI GAANNNNTTC 2 cut(s) 1053, 1171
MscI TGGCCA 1 cut(s) 1098
MseI TTAA 2 cut(s) 206, 582
Msp20I TGGCCA 1 cut(s) 1098
MspA1I CMGCKG 2 cut(s) 535, 1013
MspR9I CCNGG 2 cut(s) 101, 520
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 2 cut(s) 101, 520
MvnI CGCG 2 cut(s) 401, 567
MwoI GCNNNNNNNGC 3 cut(s) 453, 477, 695
NdeII GATC 4 cut(s) 220, 435, 500, 952
NlaIII CATG 3 cut(s) 593, 845, 1103
NlaIV GGNNCC 1 cut(s) 270
NmeAIII GCCGAG 3 cut(s) 437, 456, 667
NmuCI GTSAC 1 cut(s) 1015
PciSI GCTCTTC 1 cut(s) 1059
PctI GAATGC 1 cut(s) 346
PdmI GAANNNNTTC 2 cut(s) 1053, 1171
PfeI GAWTC 4 cut(s) 168, 242, 280, 824
PfoI TCCNGGA 1 cut(s) 518
PkrI GCNGC 1 cut(s) 640
PleI GAGTC 2 cut(s) 509, 517
PpsI GAGTC 2 cut(s) 509, 517
PshAI GACNNNNGTC 1 cut(s) 699
Psp6I CCWGG 2 cut(s) 99, 518
PspGI CCWGG 2 cut(s) 99, 518
PspN4I GGNNCC 1 cut(s) 270
PsuI RGATCY 2 cut(s) 435, 500
PvuII CAGCTG 2 cut(s) 535, 1013
RsaI GTAC 1 cut(s) 859
RsaNI GTAC 1 cut(s) 858
SapI GCTCTTC 1 cut(s) 1059
SaqAI TTAA 2 cut(s) 206, 582
SatI GCNGC 1 cut(s) 639
Sau3AI GATC 4 cut(s) 220, 435, 500, 952
SchI GAGTC 2 cut(s) 509, 517
ScrFI CCNGG 2 cut(s) 101, 520
SduI GDGCHC 1 cut(s) 916
SfaNI GCATC 3 cut(s) 456, 731, 749
SfcI CTRYAG 1 cut(s) 792
SpeI ACTAGT 1 cut(s) 1169
Sse9I AATT 8 cut(s) 19, 157, 173, 284, 305, 508, 612, 1072
SsiI CCGC 1 cut(s) 1133
SspMI CTAG 2 cut(s) 1086, 1170
StyD4I CCNGG 2 cut(s) 99, 518
StyI CCWWGG 1 cut(s) 874
TaaI ACNGT 1 cut(s) 862
TaiI ACGT 1 cut(s) 9
TaqI TCGA 9 cut(s) 23, 46, 219, 240, 365, 390, 450, 1033, 1121
TasI AATT 8 cut(s) 19, 157, 173, 284, 305, 508, 612, 1072
TfiI GAWTC 4 cut(s) 168, 242, 280, 824
Tru1I TTAA 2 cut(s) 206, 582
Tru9I TTAA 2 cut(s) 206, 582
TscAI CASTG 1 cut(s) 775
TseFI GTSAC 1 cut(s) 1015
TseI GCWGC 1 cut(s) 638
Tsp45I GTSAC 1 cut(s) 1015
TspDTI ATGAA 1 cut(s) 954
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 1 cut(s) 775
XapI RAATTY 2 cut(s) 508, 1072
XmnI GAANNNNTTC 2 cut(s) 1053, 1171
XspI CTAG 2 cut(s) 1086, 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.