Rh7DG255700

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012280
Physical Location & Seq
Forward (+)
26716077 .. 26722110
6034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG255700.1

Sequence Viewer

Length: 957 bp
ATGGACGTAAACAGAGACAATTCGACAACAACAAACCACCAACATCGAAAACCTGAGGGGCATTTCCGTCCTTTCCCACAACCCTCTGCGTCCTTCCCCCTTGGTAATAAAATGCCCCCGTTTGAAAATCCCAAACCCAAAAAGAAATCTAAAAATCAATTTTGGGGGATTCAAATTGTTTTTTTTTTTTTTTTTGGTCGTAACTTAAAAGAACATCTCGATCTCCTTCTCCTTCTTCTCGATTCAAAGCCTGAGGAGGTGTTCAAGGCACCTCTTCGGATTCAATTTGCCGTCTTTACAAGCAAATTAGAAATGGCGTCCGAGATTTTCAATGACAAGAATGCTGTTTTCAAAAAGCTGAAATCGAAGTCGGAGAACAAGATGTGCTTCGATTGTAACGCGAAGAACCCAACTTGGGCTTCAGTTACATACGGGATCTTTCTCTGCATCGATTGCTCGGCCGTCCATCGCAGTCTCGGCGTTCACATCAGCTTTGTTAGATCTACGAATTTAGACTCCTGGACTCCTGAACAGCTGAGAACAATGACCTTTGGGGGAAATAATCGCGCACAGGTTTTCTTTAAACAGCATGGATGGACAGATGGAGGCAAAATTGAGGCCAAATATACATCACGAGCTGCCGAGTTATATAGGCAGATACTTTCTAAAGAAGTAGCAAAAAGTATGGCAGAAGACGCAGGTCTTCCACCATCACCTGTTGCGTCTCAGTCGGGACAAGCATCTAATGGGCTTCCTGATATTAAGACCAGTGAAGCAATAAAAGAAAAGCCTATAGCAAGGCAAGAAACACCTGAAGTTTCTGATTCCCCCAAAGCCCCCCATGTATTTGTTACCAGTACCGTCAAGAAGCCCCTTGGTGCTAAGAAAAGTGGGAAAACTGGGGGACTTGGTGCTCGCAAACTCACTACAAAGGTTTTGATTACTAACTTAGAATGA

Protein Analysis

318

Amino Acids

35.5

Weight (kDa)

9.71

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 115 - 219 2.3e-36 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17890 AT4G17890
fragaria_vesca FvH4_5g22130
malus_domestica MD00G1122000.v1.1 MD00G1183400.v1.1 MD06G1034600.v1.1
prunus_persica Prupe.5G035300_v2.0.a1
pyrus_communis pycom06g02860 pycom16g24390
rosa_chinensis RchiOBHm_Chr7g0209771
rosa_laevigata RLG00000003094
rosa_multiflora Rmu_sc0001818.1_g000012
rosa_roxburghii Rroxscaffold_3G00248980
rosa_rugosa Rorug07G0116100
rosa_samantha Rh7AG249900 Rh7BG243800 Rh7CG265900 Rh7DG255700
rosa_wichuraiana Rw7G021170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 689
AccB1I GGYRCC 1 cut(s) 268
AccII CGCG 2 cut(s) 401, 567
AclWI GGATC 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 1 cut(s) 508
AcuI CTGAAG 2 cut(s) 405, 834
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 1 cut(s) 859
AfiI CCNNNNNNNGG 1 cut(s) 415
AgsI TTSAA 7 cut(s) 125, 173, 246, 265, 284, 331, 352
AjnI CCWGG 1 cut(s) 518
AjuI GAANNNNNNNTTGG 2 cut(s) 403, 435
AluBI AGCT 4 cut(s) 358, 492, 535, 638
AluI AGCT 4 cut(s) 358, 492, 535, 638
Alw21I GWGCWC 1 cut(s) 916
Alw26I GTCTC 3 cut(s) 9, 479, 729
AlwI GGATC 1 cut(s) 443
AoxI GGCC 2 cut(s) 459, 618
ApeKI GCWGC 1 cut(s) 638
ApoI RAATTY 1 cut(s) 508
AspLEI GCGC 1 cut(s) 569
AsuHPI GGTGA 1 cut(s) 705
AxyI CCTNAGG 2 cut(s) 54, 252
BanI GGYRCC 1 cut(s) 268
BauI CACGAG 1 cut(s) 633
BbsI GAAGAC 2 cut(s) 695, 699
Bbv12I GWGCWC 1 cut(s) 916
BbvI GCAGC 1 cut(s) 625
BccI CCATC 4 cut(s) 474, 588, 596, 718
BceAI ACGGC 2 cut(s) 275, 446
BciT130I CCWGG 1 cut(s) 520
BcoDI GTCTC 3 cut(s) 9, 479, 729
BfmI CTRYAG 1 cut(s) 792
BfuAI ACCTGC 1 cut(s) 689
BglII AGATCT 1 cut(s) 500
BisI GCNGC 1 cut(s) 639
BlsI GCNGC 1 cut(s) 640
Bme1390I CCNGG 1 cut(s) 520
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 1 cut(s) 520
BmrI ACTGGG 1 cut(s) 909
BmsI GCATC 2 cut(s) 456, 749
BmuI ACTGGG 1 cut(s) 909
BoxI GACNNNNGTC 1 cut(s) 699
BpiI GAAGAC 2 cut(s) 695, 699
Bsa29I ATCGAT 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 100, 874
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse1I ACTGG 3 cut(s) 768, 855, 904
Bse21I CCTNAGG 2 cut(s) 54, 252
BseBI CCWGG 1 cut(s) 520
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 2 cut(s) 100, 874
BseGI GGATG 1 cut(s) 599
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMII CTCAG 4 cut(s) 45, 243, 527, 740
BseNI ACTGG 3 cut(s) 768, 855, 904
BseRI GAGGAG 1 cut(s) 269
BseX3I CGGCCG 1 cut(s) 459
BseXI GCAGC 1 cut(s) 625
Bsh1236I CGCG 2 cut(s) 401, 567
Bsh1285I CGRYCG 1 cut(s) 462
BshFI GGCC 2 cut(s) 461, 620
BshNI GGYRCC 1 cut(s) 268
BshVI ATCGAT 1 cut(s) 450
BsiEI CGRYCG 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 916
BslFI GGGAC 2 cut(s) 747, 918
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 3 cut(s) 9, 479, 729
BsmBI CGTCTC 1 cut(s) 729
BsmFI GGGAC 2 cut(s) 747, 918
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 2 cut(s) 461, 620
Bsp1286I GDGCHC 1 cut(s) 916
Bsp143I GATC 3 cut(s) 220, 435, 500
BspANI GGCC 2 cut(s) 461, 620
BspCNI CTCAG 4 cut(s) 46, 244, 528, 739
BspDI ATCGAT 1 cut(s) 450
BspFNI CGCG 2 cut(s) 401, 567
BspLI GGNNCC 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 689
BspPI GGATC 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 268
BsrI ACTGG 3 cut(s) 768, 855, 904
BssECI CCNNGG 2 cut(s) 100, 874
BssMI GATC 3 cut(s) 220, 435, 500
BssNI GRCGYC 1 cut(s) 317
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 2 cut(s) 100, 874
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 1 cut(s) 520
Bst4CI ACNGT 1 cut(s) 862
Bst6I CTCTTC 1 cut(s) 279
BstACI GRCGYC 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 453
BstC8I GCNNGC 1 cut(s) 916
BstDEI CTNAG 6 cut(s) 54, 252, 536, 726, 882, 949
BstF5I GGATG 1 cut(s) 599
BstFNI CGCG 2 cut(s) 401, 567
BstHHI GCGC 1 cut(s) 569
BstKTI GATC 3 cut(s) 223, 438, 503
BstMAI GTCTC 3 cut(s) 9, 479, 729
BstMBI GATC 3 cut(s) 220, 435, 500
BstMCI CGRYCG 1 cut(s) 462
BstMWI GCNNNNNNNGC 3 cut(s) 453, 477, 695
BstNI CCWGG 1 cut(s) 520
BstPAI GACNNNNGTC 1 cut(s) 699
BstSCI CCNGG 1 cut(s) 518
BstSFI CTRYAG 1 cut(s) 792
BstUI CGCG 2 cut(s) 401, 567
BstV1I GCAGC 1 cut(s) 625
BstV2I GAAGAC 2 cut(s) 695, 699
BstX2I RGATCY 2 cut(s) 435, 500
BstYI RGATCY 2 cut(s) 435, 500
BstZI CGGCCG 1 cut(s) 459
Bsu15I ATCGAT 1 cut(s) 450
Bsu36I CCTNAGG 2 cut(s) 54, 252
BsuRI GGCC 2 cut(s) 461, 620
BsuTUI ATCGAT 1 cut(s) 450
BtgZI GCGATG 1 cut(s) 452
BtsCI GGATG 1 cut(s) 599
BtsIMutI CAGTG 1 cut(s) 775
BveI ACCTGC 1 cut(s) 689
Cac8I GCNNGC 1 cut(s) 916
CfoI GCGC 1 cut(s) 569
ClaI ATCGAT 1 cut(s) 450
CseI GACGC 4 cut(s) 78, 306, 704, 711
Csp6I GTAC 1 cut(s) 858
CviAII CATG 2 cut(s) 590, 842
CviQI GTAC 1 cut(s) 858
DdeI CTNAG 6 cut(s) 54, 252, 536, 726, 882, 949
DpnI GATC 3 cut(s) 222, 437, 502
DpnII GATC 3 cut(s) 220, 435, 500
DraI TTTAAA 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 459
EagI CGGCCG 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EclXI CGGCCG 1 cut(s) 459
Eco130I CCWWGG 2 cut(s) 100, 874
Eco52I CGGCCG 1 cut(s) 459
Eco57I CTGAAG 2 cut(s) 405, 834
Eco81I CCTNAGG 2 cut(s) 54, 252
EcoRII CCWGG 1 cut(s) 518
EcoT14I CCWWGG 2 cut(s) 100, 874
ErhI CCWWGG 2 cut(s) 100, 874
Esp3I CGTCTC 1 cut(s) 729
FaeI CATG 2 cut(s) 593, 845
FaiI YATR 8 cut(s) 430, 591, 627, 649, 651, 686, 794, 843
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FaqI GGGAC 2 cut(s) 747, 918
FatI CATG 2 cut(s) 589, 841
Fnu4HI GCNGC 1 cut(s) 639
FokI GGATG 1 cut(s) 606
Fsp4HI GCNGC 1 cut(s) 639
GlaI GCGC 1 cut(s) 568
GluI GCNGC 1 cut(s) 639
HaeIII GGCC 2 cut(s) 461, 620
HgaI GACGC 4 cut(s) 78, 306, 704, 711
HhaI GCGC 1 cut(s) 569
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 2 cut(s) 593, 845
Hin6I GCGC 1 cut(s) 567
HinP1I GCGC 1 cut(s) 567
HinfI GANTC 6 cut(s) 169, 242, 280, 515, 523, 824
HphI GGTGA 1 cut(s) 705
Hpy166II GTNNAC 2 cut(s) 10, 484
Hpy188I TCNGA 4 cut(s) 279, 322, 373, 823
Hpy188III TCNNGA 7 cut(s) 218, 239, 527, 633, 732, 755, 865
Hpy8I GTNNAC 2 cut(s) 10, 484
HpyAV CCTTC 3 cut(s) 103, 236, 242
HpyCH4III ACNGT 1 cut(s) 862
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 1 cut(s) 447
HpyF10VI GCNNNNNNNGC 3 cut(s) 453, 477, 695
HpyF3I CTNAG 6 cut(s) 54, 252, 536, 726, 882, 949
HpySE526I ACGT 1 cut(s) 6
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 2 cut(s) 593, 845
HspAI GCGC 1 cut(s) 567
Kzo9I GATC 3 cut(s) 220, 435, 500
Lsp1109I GCAGC 1 cut(s) 625
LweI GCATC 2 cut(s) 456, 749
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 4 cut(s) 200, 395, 424, 850
MalI GATC 3 cut(s) 222, 437, 502
MboI GATC 3 cut(s) 220, 435, 500
MboII GAAGA 5 cut(s) 227, 266, 415, 695, 704
MflI RGATCY 2 cut(s) 435, 500
MhlI GDGCHC 1 cut(s) 916
MluCI AATT 7 cut(s) 19, 158, 174, 284, 305, 508, 612
MlyI GAGTC 2 cut(s) 509, 517
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 7 cut(s) 49, 94, 247, 250, 282, 599, 610
MseI TTAA 3 cut(s) 206, 582, 762
MspA1I CMGCKG 1 cut(s) 535
MspR9I CCNGG 1 cut(s) 520
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 520
MvnI CGCG 2 cut(s) 401, 567
MwoI GCNNNNNNNGC 3 cut(s) 453, 477, 695
NdeII GATC 3 cut(s) 220, 435, 500
NlaIII CATG 2 cut(s) 593, 845
NlaIV GGNNCC 1 cut(s) 270
NmeAIII GCCGAG 3 cut(s) 437, 456, 667
PctI GAATGC 1 cut(s) 346
PfeI GAWTC 4 cut(s) 169, 242, 280, 824
PfoI TCCNGGA 1 cut(s) 518
PkrI GCNGC 1 cut(s) 640
PleI GAGTC 2 cut(s) 509, 517
PpsI GAGTC 2 cut(s) 509, 517
PshAI GACNNNNGTC 1 cut(s) 699
Psp6I CCWGG 1 cut(s) 518
PspGI CCWGG 1 cut(s) 518
PspN4I GGNNCC 1 cut(s) 270
PsuI RGATCY 2 cut(s) 435, 500
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 1 cut(s) 859
RsaNI GTAC 1 cut(s) 858
SaqAI TTAA 3 cut(s) 206, 582, 762
SatI GCNGC 1 cut(s) 639
Sau3AI GATC 3 cut(s) 220, 435, 500
SchI GAGTC 2 cut(s) 509, 517
ScrFI CCNGG 1 cut(s) 520
SduI GDGCHC 1 cut(s) 916
SfaNI GCATC 2 cut(s) 456, 749
SfcI CTRYAG 1 cut(s) 792
Sse9I AATT 7 cut(s) 19, 158, 174, 284, 305, 508, 612
StyD4I CCNGG 1 cut(s) 518
StyI CCWWGG 2 cut(s) 100, 874
TaaI ACNGT 1 cut(s) 862
TaiI ACGT 1 cut(s) 9
TaqI TCGA 7 cut(s) 23, 46, 219, 240, 365, 390, 450
TasI AATT 7 cut(s) 19, 158, 174, 284, 305, 508, 612
TfiI GAWTC 4 cut(s) 169, 242, 280, 824
Tru1I TTAA 3 cut(s) 206, 582, 762
Tru9I TTAA 3 cut(s) 206, 582, 762
TscAI CASTG 1 cut(s) 775
TseI GCWGC 1 cut(s) 638
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 1 cut(s) 775
XapI RAATTY 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.