MD00G1183400.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012280
Physical Location & Seq
Reverse (-)
43329510 .. 43335499
5990 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1183400.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGGCGTCCGAAATCTCAAACGACAAGACCGCTGTTTTCAGAAAACTGAAAGCAAGGTCAGAAAACAAGATGTGTTTCGATTGTAATGCAAAGAACCCGACTTGGGCGTCGGTTACATATGGGATCTTTCTCTGCATCGATTGCTCGGCCGTTCATCGCAGTCTCGGCGTTCATATCAGCTTTGTTAGATCTACAAATTTAGACTCGTGGACTCCTGAACAGCTGAGAACGATGAGCTTCGGGGGAAACAACCGTGCACAAGTTTTCTTTAAGCAGCATGGATGGACGGATGGAGGCAAAATTGAGGCTAAGTATACATCAAGAGCTGCCGAGTTGTATAGGCAAATACTTTCTAAAGAAGTTGTCAAAAGTATGGCAGAAGAGGCAGGTCTTCCACCATCACCTGTTACATCTCAGTCAGGACAAGCATCTAATGGGATTCTTGATGTTAAGACCCAGGAAGCTCCAAAAGAGAACTCTATAGCAAGGAAAGAAACGCCTGAAATCGCTGCTTCCCAGAAAGCCCCCCATACATATGTTACCAGTACTGTTAAGAAGCCTCTTGGTGGTGCTAAGAAAATTGGGAAGACTGGGGGTCTTGGTGCTCGCAAACTTACTACAAAGCCAAATGAAAGCCTGTACGATCAGAAACCTGAAGATCCAGTTGTCCCACTGCCAGTGTCTTCCTCAACTAACAGCACTCCAACAGCTGGATCATCCTTTGCATCTCGCTTTGAGTATGTAGAAATTGTCCAAAATACTGAGACAAATCCCACTGGCGCTCATGTAATTAGCCATATTGCTCCACCAAAGTCATCAAATTTCTTTGCAGATTTTGGAATGGACAGTAACTTTCCCAAGAAAACTAGTTCAAATACCTCAAAAGTTCAAATTCAGGAGTCTGATGAAGCAAGAAAGAAATTCACAAATGCAAAATCTATCTCTTCCGCACAATTTTTTGGTGACCAAAGCAGATCTGCTGATGTTGATGCTCAAACCTCCTTGCAGAAGTTCTCGGGTTCTGCAGCCATCTCCAGCGCTGATCTATTTGGTGACAGGGGAGATAATTCAGCTATTGATCTTACTGCAAGCGACCTAATCAACCGACTGTCGTTTCAGGCACAACAGGATCTCTCTTCCTTAACGAATATCGCGGGAGAGACCGGGAAGAAGCTTAGTTCCTTGGCGTCCAATTTAATGACGGATCTTCAGGACAGAATCCTGTGA

Protein Analysis

409

Amino Acids

44.04

Weight (kDa)

8.95

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 12 - 115 9.5e-36 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17890 AT4G17890
fragaria_vesca FvH4_5g22130
malus_domestica MD00G1122000.v1.1 MD00G1183400.v1.1 MD06G1034600.v1.1
prunus_persica Prupe.5G035300_v2.0.a1
pyrus_communis pycom06g02860 pycom16g24390
rosa_chinensis RchiOBHm_Chr7g0209771
rosa_laevigata RLG00000003094
rosa_multiflora Rmu_sc0001818.1_g000012
rosa_roxburghii Rroxscaffold_3G00248980
rosa_rugosa Rorug07G0116100
rosa_samantha Rh7AG249900 Rh7BG243800 Rh7CG265900 Rh7DG255700
rosa_wichuraiana Rw7G021170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 106
Acc36I ACCTGC 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 710
AccI GTMKAC 1 cut(s) 314
AccII CGCG 1 cut(s) 1154
AciI CCGC 3 cut(s) 30, 948, 1154
AclWI GGATC 5 cut(s) 131, 653, 721, 1137, 1212
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 4 cut(s) 196, 820, 891, 920
AcuI CTGAAG 2 cut(s) 675, 1193
AcyI GRCGYC 3 cut(s) 5, 107, 1187
AfaI GTAC 2 cut(s) 547, 641
AfeI AGCGCT 1 cut(s) 1039
AfiI CCNNNNNNNGG 3 cut(s) 103, 566, 710
AgsI TTSAA 2 cut(s) 873, 890
AhdI GACNNNNNGTC 1 cut(s) 594
AhlI ACTAGT 1 cut(s) 866
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 8 cut(s) 180, 223, 237, 326, 464, 710, 1073, 1174
AluI AGCT 8 cut(s) 180, 223, 237, 326, 464, 710, 1073, 1174
Alw21I GWGCWC 2 cut(s) 259, 607
Alw26I GTCTC 3 cut(s) 167, 758, 1154
Alw44I GTGCAC 1 cut(s) 255
AlwI GGATC 5 cut(s) 131, 653, 721, 1137, 1212
Ama87I CYCGRG 1 cut(s) 1015
Aor51HI AGCGCT 1 cut(s) 1039
AoxI GGCC 1 cut(s) 147
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 4 cut(s) 274, 326, 509, 1025
ApoI RAATTY 4 cut(s) 196, 820, 891, 920
AspLEI GCGC 2 cut(s) 782, 1040
AsuC2I CCSGG 1 cut(s) 1165
AsuHPI GGTGA 3 cut(s) 393, 974, 1064
AvaI CYCGRG 1 cut(s) 1015
BaeGI GKGCMC 1 cut(s) 259
BauI CACGAG 1 cut(s) 205
BbsI GAAGAC 3 cut(s) 383, 593, 675
Bbv12I GWGCWC 2 cut(s) 259, 607
BbvI GCAGC 4 cut(s) 286, 313, 496, 1037
BccI CCATC 4 cut(s) 276, 284, 406, 1037
BceAI ACGGC 1 cut(s) 134
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 1 cut(s) 458
BcnI CCSGG 1 cut(s) 1165
BcoDI GTCTC 3 cut(s) 167, 758, 1154
BcuI ACTAGT 1 cut(s) 866
BfaI CTAG 1 cut(s) 867
BfmI CTRYAG 2 cut(s) 480, 1023
BfoI RGCGCY 2 cut(s) 783, 1041
BfuAI ACCTGC 1 cut(s) 377
BglII AGATCT 2 cut(s) 188, 974
BisI GCNGC 4 cut(s) 275, 327, 510, 1026
BlsI GCNGC 4 cut(s) 276, 328, 511, 1027
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 2 cut(s) 458, 1165
BmeRI GACNNNNNGTC 1 cut(s) 594
BmeT110I CYCGRG 1 cut(s) 1015
BmrFI CCNGG 2 cut(s) 458, 1165
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 4 cut(s) 144, 437, 734, 979
BmuI ACTGGG 1 cut(s) 600
BpiI GAAGAC 3 cut(s) 383, 593, 675
BpmI CTGGAG 1 cut(s) 1018
BpuMI CCSGG 1 cut(s) 1165
Bsa29I ATCGAT 1 cut(s) 138
BsaHI GRCGYC 3 cut(s) 5, 107, 1187
BsaI GGTCTC 1 cut(s) 1154
BsaJI CCNNGG 2 cut(s) 456, 1182
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 566, 710
Bse1I ACTGG 5 cut(s) 543, 595, 662, 677, 781
BseBI CCWGG 1 cut(s) 458
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 456, 1182
BseGI GGATG 3 cut(s) 287, 295, 716
BseLI CCNNNNNNNGG 3 cut(s) 103, 566, 710
BseMII CTCAG 3 cut(s) 215, 428, 753
BseNI ACTGG 5 cut(s) 543, 595, 662, 677, 781
BseSI GKGCMC 1 cut(s) 259
BseX3I CGGCCG 1 cut(s) 147
BseXI GCAGC 4 cut(s) 286, 313, 496, 1037
Bsh1236I CGCG 1 cut(s) 1154
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 1 cut(s) 149
BshVI ATCGAT 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 259, 607
BsiHKCI CYCGRG 1 cut(s) 1015
BsiSI CCGG 1 cut(s) 1164
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 3 cut(s) 103, 566, 710
BsmAI GTCTC 3 cut(s) 167, 758, 1154
BsmFI GGGAC 1 cut(s) 653
BsnI GGCC 1 cut(s) 149
Bso31I GGTCTC 1 cut(s) 1154
BsoBI CYCGRG 1 cut(s) 1015
Bsp1286I GDGCHC 2 cut(s) 259, 607
BspACI CCGC 3 cut(s) 30, 948, 1154
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 3 cut(s) 216, 427, 754
BspDI ATCGAT 1 cut(s) 138
BspFNI CGCG 1 cut(s) 1154
BspMAI CTGCAG 1 cut(s) 1027
BspMI ACCTGC 1 cut(s) 377
BspPI GGATC 5 cut(s) 131, 653, 721, 1137, 1212
BspTNI GGTCTC 1 cut(s) 1154
BsrI ACTGG 5 cut(s) 543, 595, 662, 677, 781
BssECI CCNNGG 2 cut(s) 456, 1182
BssNAI GTATAC 1 cut(s) 315
BssNI GRCGYC 3 cut(s) 5, 107, 1187
BssSI CACGAG 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 1182
Bst1107I GTATAC 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 205
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 4 cut(s) 254, 550, 848, 1110
Bst6I CTCTTC 3 cut(s) 375, 949, 1141
BstACI GRCGYC 3 cut(s) 5, 107, 1187
BstAPI GCANNNNNTGC 1 cut(s) 141
BstC8I GCNNGC 2 cut(s) 607, 1090
BstDEI CTNAG 6 cut(s) 224, 309, 414, 573, 762, 1175
BstEII GGTNACC 1 cut(s) 962
BstF5I GGATG 3 cut(s) 287, 295, 716
BstFNI CGCG 1 cut(s) 1154
BstH2I RGCGCY 2 cut(s) 783, 1041
BstHHI GCGC 2 cut(s) 782, 1040
BstMAI GTCTC 3 cut(s) 167, 758, 1154
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 3 cut(s) 141, 165, 383
BstNI CCWGG 1 cut(s) 458
BstPI GGTNACC 1 cut(s) 962
BstSCI CCNGG 2 cut(s) 456, 1163
BstSFI CTRYAG 2 cut(s) 480, 1023
BstSLI GKGCMC 1 cut(s) 259
BstUI CGCG 1 cut(s) 1154
BstV1I GCAGC 4 cut(s) 286, 313, 496, 1037
BstV2I GAAGAC 3 cut(s) 383, 593, 675
BstX2I RGATCY 6 cut(s) 123, 188, 658, 974, 1129, 1204
BstYI RGATCY 6 cut(s) 123, 188, 658, 974, 1129, 1204
BstZ17I GTATAC 1 cut(s) 315
BstZI CGGCCG 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 138
BsuRI GGCC 1 cut(s) 149
BsuTUI ATCGAT 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 140
BtsCI GGATG 3 cut(s) 287, 295, 716
BtsI GCAGTG 1 cut(s) 671
BtsIMutI CAGTG 3 cut(s) 671, 684, 774
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 2 cut(s) 607, 1090
CfoI GCGC 2 cut(s) 782, 1040
ClaI ATCGAT 1 cut(s) 138
CseI GACGC 2 cut(s) 96, 1176
Csp6I GTAC 2 cut(s) 546, 640
CviAII CATG 2 cut(s) 278, 785
CviQI GTAC 2 cut(s) 546, 640
DdeI CTNAG 6 cut(s) 224, 309, 414, 573, 762, 1175
DrdI GACNNNNNNGTC 1 cut(s) 106
DriI GACNNNNNGTC 1 cut(s) 594
DseDI GACNNNNNNGTC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 147
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 3 cut(s) 375, 949, 1141
Eam1105I GACNNNNNGTC 1 cut(s) 594
EarI CTCTTC 3 cut(s) 375, 949, 1141
EclXI CGGCCG 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 1182
Eco31I GGTCTC 1 cut(s) 1154
Eco47III AGCGCT 1 cut(s) 1039
Eco52I CGGCCG 1 cut(s) 147
Eco57I CTGAAG 2 cut(s) 675, 1193
Eco88I CYCGRG 1 cut(s) 1015
Eco91I GGTNACC 1 cut(s) 962
EcoO65I GGTNACC 1 cut(s) 962
EcoRII CCWGG 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 1182
ErhI CCWWGG 1 cut(s) 1182
FaeI CATG 2 cut(s) 281, 788
FaqI GGGAC 1 cut(s) 653
FatI CATG 2 cut(s) 277, 784
FauI CCCGC 1 cut(s) 1147
FauNDI CATATG 2 cut(s) 118, 535
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 4 cut(s) 275, 327, 510, 1026
FokI GGATG 3 cut(s) 294, 302, 703
Fsp4HI GCNGC 4 cut(s) 275, 327, 510, 1026
FspBI CTAG 1 cut(s) 867
GlaI GCGC 2 cut(s) 781, 1039
GluI GCNGC 4 cut(s) 275, 327, 510, 1026
GsuI CTGGAG 1 cut(s) 1018
HaeII RGCGCY 2 cut(s) 783, 1041
HaeIII GGCC 1 cut(s) 149
HapII CCGG 1 cut(s) 1164
HgaI GACGC 2 cut(s) 96, 1176
HhaI GCGC 2 cut(s) 782, 1040
Hin1I GRCGYC 3 cut(s) 5, 107, 1187
Hin1II CATG 2 cut(s) 281, 788
Hin6I GCGC 2 cut(s) 780, 1038
HinP1I GCGC 2 cut(s) 780, 1038
HindIII AAGCTT 1 cut(s) 1172
HinfI GANTC 5 cut(s) 203, 211, 439, 899, 1218
HpaII CCGG 1 cut(s) 1164
HphI GGTGA 3 cut(s) 393, 974, 1064
Hpy166II GTNNAC 3 cut(s) 210, 257, 315
Hpy188I TCNGA 5 cut(s) 10, 41, 61, 648, 904
Hpy188III TCNNGA 6 cut(s) 215, 321, 420, 443, 896, 1211
Hpy8I GTNNAC 3 cut(s) 210, 257, 315
Hpy99I CGWCG 1 cut(s) 112
HpyCH4III ACNGT 4 cut(s) 254, 550, 848, 1110
HpyCH4V TGCA 9 cut(s) 89, 135, 257, 725, 830, 932, 1006, 1025, 1088
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 165, 383
HpyF3I CTNAG 6 cut(s) 224, 309, 414, 573, 762, 1175
Hsp92I GRCGYC 3 cut(s) 5, 107, 1187
Hsp92II CATG 2 cut(s) 281, 788
HspAI GCGC 2 cut(s) 780, 1038
LmnI GCTCC 2 cut(s) 469, 808
Lsp1109I GCAGC 4 cut(s) 286, 313, 496, 1037
LweI GCATC 4 cut(s) 144, 437, 734, 979
MaeI CTAG 1 cut(s) 867
MaeIII GTNAC 6 cut(s) 112, 406, 538, 848, 962, 1052
MboII GAAGA 9 cut(s) 383, 392, 598, 668, 675, 936, 1128, 1180, 1199
MflI RGATCY 6 cut(s) 123, 188, 658, 974, 1129, 1204
MhlI GDGCHC 2 cut(s) 259, 607
MlyI GAGTC 3 cut(s) 197, 205, 908
MmeI TCCRAC 1 cut(s) 728
MnlI CCTC 7 cut(s) 287, 298, 376, 570, 697, 889, 1009
MseI TTAA 5 cut(s) 270, 450, 552, 1142, 1196
MslI CAYNNNNRTG 1 cut(s) 534
MspA1I CMGCKG 3 cut(s) 32, 223, 710
MspI CCGG 1 cut(s) 1164
MspR9I CCNGG 2 cut(s) 458, 1165
MvaI CCWGG 1 cut(s) 458
MvnI CGCG 1 cut(s) 1154
MwoI GCNNNNNNNGC 3 cut(s) 141, 165, 383
NciI CCSGG 1 cut(s) 1165
NdeI CATATG 2 cut(s) 118, 535
NlaIII CATG 2 cut(s) 281, 788
NmeAIII GCCGAG 3 cut(s) 125, 144, 355
NmuCI GTSAC 2 cut(s) 962, 1052
PfeI GAWTC 2 cut(s) 439, 1218
PflMI CCANNNNNTGG 1 cut(s) 710
PkrI GCNGC 4 cut(s) 276, 328, 511, 1027
PleI GAGTC 3 cut(s) 197, 205, 907
PpsI GAGTC 3 cut(s) 197, 205, 907
Psp6I CCWGG 1 cut(s) 456
PspEI GGTNACC 1 cut(s) 962
PspGI CCWGG 1 cut(s) 456
PstI CTGCAG 1 cut(s) 1027
PsuI RGATCY 6 cut(s) 123, 188, 658, 974, 1129, 1204
PvuII CAGCTG 2 cut(s) 223, 710
RsaI GTAC 2 cut(s) 547, 641
RsaNI GTAC 2 cut(s) 546, 640
RseI CAYNNNNRTG 1 cut(s) 534
SaqAI TTAA 5 cut(s) 270, 450, 552, 1142, 1196
SatI GCNGC 4 cut(s) 275, 327, 510, 1026
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 3 cut(s) 197, 205, 908
ScrFI CCNGG 2 cut(s) 458, 1165
SduI GDGCHC 2 cut(s) 259, 607
SfaNI GCATC 4 cut(s) 144, 437, 734, 979
SfcI CTRYAG 2 cut(s) 480, 1023
SmiMI CAYNNNNRTG 1 cut(s) 534
SpeI ACTAGT 1 cut(s) 866
SsiI CCGC 3 cut(s) 30, 948, 1154
SspMI CTAG 1 cut(s) 867
StyD4I CCNGG 2 cut(s) 456, 1163
StyI CCWWGG 1 cut(s) 1182
TaaI ACNGT 4 cut(s) 254, 550, 848, 1110
TaqI TCGA 2 cut(s) 78, 138
TatI WGTACW 1 cut(s) 545
TfiI GAWTC 2 cut(s) 439, 1218
Tru1I TTAA 5 cut(s) 270, 450, 552, 1142, 1196
Tru9I TTAA 5 cut(s) 270, 450, 552, 1142, 1196
TscAI CASTG 3 cut(s) 678, 684, 781
TseFI GTSAC 2 cut(s) 962, 1052
TseI GCWGC 4 cut(s) 274, 326, 509, 1025
Tsp45I GTSAC 2 cut(s) 962, 1052
TspDTI ATGAA 4 cut(s) 143, 161, 645, 921
TspGWI ACGGA 2 cut(s) 302, 1217
TspRI CASTG 3 cut(s) 678, 684, 781
Van91I CCANNNNNTGG 1 cut(s) 710
VneI GTGCAC 1 cut(s) 255
XapI RAATTY 4 cut(s) 196, 820, 891, 920
XmiI GTMKAC 1 cut(s) 314
XspI CTAG 1 cut(s) 867
ZrmI AGTACT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.