pycom06g02860

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012280
Physical Location & Seq
Forward (+)
3498593 .. 3502025
3433 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g02860.1

Sequence Viewer

Length: 903 bp
ATGGCGTCCGAAATCTTCAACGACAAGACCGCTGTTTTCAGAAAACTGAAAGCCAAGGCAGAAAACAAGATGTGTTTCGATTGTAATGCGAAGAACCCGACTTGGGCATCGGTTACATATGGGATCTTTCTCTGCATCGATTGCTCCGCCGTTCATCGTAGTCTCGGCGTTCACATCAGCTTTGTTAGATCTACAAATTTAGACTCATGGACTCCTGAACAGCTGAGAACGATGAGCTTTGGAGGGAACAACCGTGCACAAGTTTTCTTTAAGCAGCATGGATGGACAGATGGAGGCAAAATTGAGGCTAAGTACACATCAAGAGCTGCCGAGTTGTATAGGCATATACTTACCAAAGAAGTTGCCAAAAGTATGGCAGAAGAGATAGGTCTTCCATCATCTCCTGTTGCATCTCAGTCAGGACAAGCATCTAATGGGCTTCCTGATGTCAAGACCCATGAAGCTCCAAAAGAGAACTCTATAGCAAGGAAAGAAACACCTGAAATCGCTGCTTCCCCGAAAGCCCCTCATACATATGTTACCAGTACTGTTAAGAAGCCTCTTGGTGGTGCTAAGAAAACTGGGAAGACCGGGGGGCTTGGTGCTCGCAAACTTACTACAAAGCCAAGTGAAAGCCTGTACGATCAGAAACCTGAAGAGCCAGTTGCCCCAGTTCCAGTGCCTTCCTCAACTAACAGCACTCCAACAGCTGGATCATCCTTTGCATCTCGATTTGAGTATGTAGAAATTGTCCAAAATACTGAGACAAATCCCACTGGTGCTCATGTGATTAGCCATATTGCTCCGCCAAAGTCATCAAATTTCTTTTCGGATTTTGGAATGGACAATAGTTTCCCCAAGAGAACTAGTTCAAATACCTCAAAAGTTCAAGTAAGAAACTGA

Protein Analysis

301

Amino Acids

32.54

Weight (kDa)

9.45

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 12 - 114 5e-36 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17890 AT4G17890
fragaria_vesca FvH4_5g22130
malus_domestica MD00G1122000.v1.1 MD00G1183400.v1.1 MD06G1034600.v1.1
prunus_persica Prupe.5G035300_v2.0.a1
pyrus_communis pycom06g02860 pycom16g24390
rosa_chinensis RchiOBHm_Chr7g0209771
rosa_laevigata RLG00000003094
rosa_multiflora Rmu_sc0001818.1_g000012
rosa_roxburghii Rroxscaffold_3G00248980
rosa_rugosa Rorug07G0116100
rosa_samantha Rh7AG249900 Rh7BG243800 Rh7CG265900 Rh7DG255700
rosa_wichuraiana Rw7G021170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 710
AciI CCGC 3 cut(s) 30, 147, 806
AclWI GGATC 2 cut(s) 131, 721
AcsI RAATTY 2 cut(s) 196, 820
AcuI CTGAAG 1 cut(s) 675
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 314, 547, 641
AfiI CCNNNNNNNGG 3 cut(s) 103, 566, 710
AgsI TTSAA 3 cut(s) 19, 873, 890
AhlI ACTAGT 1 cut(s) 866
AluBI AGCT 6 cut(s) 180, 223, 237, 326, 464, 710
AluI AGCT 6 cut(s) 180, 223, 237, 326, 464, 710
Alw21I GWGCWC 3 cut(s) 259, 607, 784
Alw26I GTCTC 2 cut(s) 167, 758
Alw44I GTGCAC 1 cut(s) 255
AlwI GGATC 2 cut(s) 131, 721
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 3 cut(s) 274, 326, 509
ApoI RAATTY 2 cut(s) 196, 820
Asp700I GAANNNNTTC 1 cut(s) 868
AsuC2I CCSGG 1 cut(s) 592
BaeGI GKGCMC 1 cut(s) 259
BbsI GAAGAC 2 cut(s) 383, 593
Bbv12I GWGCWC 3 cut(s) 259, 607, 784
BbvI GCAGC 3 cut(s) 286, 313, 496
BccI CCATC 3 cut(s) 276, 284, 403
BceAI ACGGC 1 cut(s) 134
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BcnI CCSGG 1 cut(s) 592
BcoDI GTCTC 2 cut(s) 167, 758
BcuI ACTAGT 1 cut(s) 866
BfaI CTAG 1 cut(s) 867
BfmI CTRYAG 1 cut(s) 480
BglII AGATCT 1 cut(s) 188
BisI GCNGC 3 cut(s) 275, 327, 510
BlsI GCNGC 3 cut(s) 276, 328, 511
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 1 cut(s) 592
BmrFI CCNGG 1 cut(s) 592
BmrI ACTGGG 2 cut(s) 591, 665
BmsI GCATC 5 cut(s) 116, 144, 419, 437, 734
BmuI ACTGGG 2 cut(s) 591, 665
BpiI GAAGAC 2 cut(s) 383, 593
BpuMI CCSGG 1 cut(s) 592
Bsa29I ATCGAT 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 54, 591
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 566, 710
Bse1I ACTGG 6 cut(s) 543, 586, 662, 671, 677, 781
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 54, 591
BseGI GGATG 2 cut(s) 287, 716
BseLI CCNNNNNNNGG 3 cut(s) 103, 566, 710
BseMII CTCAG 3 cut(s) 215, 428, 753
BseNI ACTGG 6 cut(s) 543, 586, 662, 671, 677, 781
BseSI GKGCMC 1 cut(s) 259
BseXI GCAGC 3 cut(s) 286, 313, 496
BshVI ATCGAT 1 cut(s) 138
BsiHKAI GWGCWC 3 cut(s) 259, 607, 784
BsiSI CCGG 1 cut(s) 591
BslI CCNNNNNNNGG 3 cut(s) 103, 566, 710
BsmAI GTCTC 2 cut(s) 167, 758
Bsp1286I GDGCHC 3 cut(s) 259, 607, 784
Bsp143I GATC 4 cut(s) 123, 188, 643, 713
BspACI CCGC 3 cut(s) 30, 147, 806
BspCNI CTCAG 3 cut(s) 216, 427, 754
BspDI ATCGAT 1 cut(s) 138
BspPI GGATC 2 cut(s) 131, 721
BspQI GCTCTTC 1 cut(s) 651
BsrI ACTGG 6 cut(s) 543, 586, 662, 671, 677, 781
BssECI CCNNGG 2 cut(s) 54, 591
BssMI GATC 4 cut(s) 123, 188, 643, 713
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 54
Bst4CI ACNGT 2 cut(s) 254, 550
Bst6I CTCTTC 2 cut(s) 375, 651
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 607
BstDEI CTNAG 5 cut(s) 224, 309, 414, 573, 762
BstF5I GGATG 2 cut(s) 287, 716
BstKTI GATC 4 cut(s) 126, 191, 646, 716
BstMAI GTCTC 2 cut(s) 167, 758
BstMBI GATC 4 cut(s) 123, 188, 643, 713
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 590
BstSFI CTRYAG 1 cut(s) 480
BstSLI GKGCMC 1 cut(s) 259
BstV1I GCAGC 3 cut(s) 286, 313, 496
BstV2I GAAGAC 2 cut(s) 383, 593
BstX2I RGATCY 2 cut(s) 123, 188
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 2 cut(s) 123, 188
Bsu15I ATCGAT 1 cut(s) 138
BsuTUI ATCGAT 1 cut(s) 138
BtsCI GGATG 2 cut(s) 287, 716
BtsIMutI CAGTG 2 cut(s) 684, 774
Cac8I GCNNGC 1 cut(s) 607
ClaI ATCGAT 1 cut(s) 138
Csp6I GTAC 3 cut(s) 313, 546, 640
CviAII CATG 4 cut(s) 207, 278, 458, 785
CviQI GTAC 3 cut(s) 313, 546, 640
DdeI CTNAG 5 cut(s) 224, 309, 414, 573, 762
DpnI GATC 4 cut(s) 125, 190, 645, 715
DpnII GATC 4 cut(s) 123, 188, 643, 713
Eam1104I CTCTTC 2 cut(s) 375, 651
EarI CTCTTC 2 cut(s) 375, 651
EciI GGCGGA 2 cut(s) 136, 795
Eco130I CCWWGG 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 675
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 4 cut(s) 210, 281, 461, 788
FatI CATG 4 cut(s) 206, 277, 457, 784
FauNDI CATATG 2 cut(s) 118, 535
Fnu4HI GCNGC 3 cut(s) 275, 327, 510
FokI GGATG 2 cut(s) 294, 703
Fsp4HI GCNGC 3 cut(s) 275, 327, 510
FspBI CTAG 1 cut(s) 867
GluI GCNGC 3 cut(s) 275, 327, 510
HapII CCGG 1 cut(s) 591
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 210, 281, 461, 788
HinfI GANTC 2 cut(s) 203, 211
HpaII CCGG 1 cut(s) 591
Hpy166II GTNNAC 3 cut(s) 172, 257, 315
Hpy188I TCNGA 4 cut(s) 10, 41, 648, 832
Hpy188III TCNNGA 6 cut(s) 215, 321, 420, 443, 451, 729
Hpy8I GTNNAC 3 cut(s) 172, 257, 315
HpyAV CCTTC 1 cut(s) 693
HpyCH4III ACNGT 2 cut(s) 254, 550
HpyCH4V TGCA 4 cut(s) 135, 257, 410, 725
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 5 cut(s) 224, 309, 414, 573, 762
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 210, 281, 461, 788
Kzo9I GATC 4 cut(s) 123, 188, 643, 713
LguI GCTCTTC 1 cut(s) 651
LmnI GCTCC 3 cut(s) 149, 469, 808
Lsp1109I GCAGC 3 cut(s) 286, 313, 496
LweI GCATC 5 cut(s) 116, 144, 419, 437, 734
MaeI CTAG 1 cut(s) 867
MaeIII GTNAC 2 cut(s) 112, 538
MalI GATC 4 cut(s) 125, 190, 645, 715
MboI GATC 4 cut(s) 123, 188, 643, 713
MboII GAAGA 6 cut(s) 7, 103, 383, 392, 598, 668
MflI RGATCY 2 cut(s) 123, 188
MhlI GDGCHC 3 cut(s) 259, 607, 784
MluCI AATT 4 cut(s) 196, 300, 747, 820
MlyI GAGTC 2 cut(s) 197, 205
MmeI TCCRAC 1 cut(s) 728
MnlI CCTC 7 cut(s) 236, 287, 298, 537, 570, 697, 889
MroXI GAANNNNTTC 1 cut(s) 868
MseI TTAA 2 cut(s) 270, 552
MslI CAYNNNNRTG 1 cut(s) 534
MspA1I CMGCKG 3 cut(s) 32, 223, 710
MspI CCGG 1 cut(s) 591
MspR9I CCNGG 1 cut(s) 592
MwoI GCNNNNNNNGC 1 cut(s) 141
NciI CCSGG 1 cut(s) 592
NdeI CATATG 2 cut(s) 118, 535
NdeII GATC 4 cut(s) 123, 188, 643, 713
NlaIII CATG 4 cut(s) 210, 281, 461, 788
NmeAIII GCCGAG 2 cut(s) 144, 355
PciSI GCTCTTC 1 cut(s) 651
PdmI GAANNNNTTC 1 cut(s) 868
PflMI CCANNNNNTGG 1 cut(s) 710
PkrI GCNGC 3 cut(s) 276, 328, 511
PleI GAGTC 2 cut(s) 197, 205
PpsI GAGTC 2 cut(s) 197, 205
PsuI RGATCY 2 cut(s) 123, 188
PvuII CAGCTG 2 cut(s) 223, 710
RsaI GTAC 3 cut(s) 314, 547, 641
RsaNI GTAC 3 cut(s) 313, 546, 640
RseI CAYNNNNRTG 1 cut(s) 534
SapI GCTCTTC 1 cut(s) 651
SaqAI TTAA 2 cut(s) 270, 552
SatI GCNGC 3 cut(s) 275, 327, 510
Sau3AI GATC 4 cut(s) 123, 188, 643, 713
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 2 cut(s) 197, 205
ScrFI CCNGG 1 cut(s) 592
SduI GDGCHC 3 cut(s) 259, 607, 784
SfaNI GCATC 5 cut(s) 116, 144, 419, 437, 734
SfcI CTRYAG 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 534
SpeI ACTAGT 1 cut(s) 866
Sse9I AATT 4 cut(s) 196, 300, 747, 820
SsiI CCGC 3 cut(s) 30, 147, 806
SspMI CTAG 1 cut(s) 867
StyD4I CCNGG 1 cut(s) 590
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 2 cut(s) 254, 550
TaqI TCGA 3 cut(s) 78, 138, 730
TasI AATT 4 cut(s) 196, 300, 747, 820
TatI WGTACW 2 cut(s) 312, 545
Tru1I TTAA 2 cut(s) 270, 552
Tru9I TTAA 2 cut(s) 270, 552
TscAI CASTG 2 cut(s) 684, 781
TseI GCWGC 3 cut(s) 274, 326, 509
TspDTI ATGAA 2 cut(s) 143, 474
TspRI CASTG 2 cut(s) 684, 781
Van91I CCANNNNNTGG 1 cut(s) 710
VneI GTGCAC 1 cut(s) 255
XapI RAATTY 2 cut(s) 196, 820
XmnI GAANNNNTTC 1 cut(s) 868
XspI CTAG 1 cut(s) 867
ZrmI AGTACT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.