MD06G1034600.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012280
Physical Location & Seq
Forward (+)
4308089 .. 4313209
5121 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1034600.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGGCGTCCGAAATCTTCAACGACAAGACCGCTGTTTTCAGAAAACTGAAAGCGAAGGCAGAAAACAAGATGTGTTTCGATTGTAATGCGAAGAACCCGACTTGGGCATCGGTTACATATGGGATCTTTCTCTGCATCGATTGCTCCGCTGTTCATCGTAGTCTCGGCGTTCACATCAGCTTTGTTAGATCTACAAATTTAGACTCGTGGACTCCTGAACAGCTGAGAACGATGAGCTTTGGAGGAAACAACCGTGCACAAGTTTTCTTTAAGCAGCATGGATGGACGGATGGAGGCAAAATTGAGGCTAAGTACACATCAAGAGCTGCCGAGTTGTATAGGCATATACTTACCAAAGAAGTTGCCAAAAGTATGGCAGAAGAGGCAGGTCTTCCATCATCACCTGTTGCATCTCAGTCAGGACAAGCATCTAATGGGCTTCCTGATGTCAAGACCCATGAAGCTCCAAAAGAGAACTCTATAGCAAGGAAAGAAACACCTGAAATCGCTGCTTCCCCGAAAGCCCCTCACACATATGTTACCAGTACTGTTAAGAAGCCTCTTGGTGGTGCTAAGAAAACTGGGAAGACCGGGGGGCTTGGTGCTCGCAAACTTACTACAAAGCCAAGTGAAAGCCTGTACGATCAGAAACCTGAAGAGCCAGTTGCCCCAGTTCCAGTGCCTTCCTCAACTAACAGCACTCCAACAGCTGGATCATCCTTTGCATCTCGATTTGAGTATGTAGAAATTGTCCAAAATACTGAGACAAATCCCACTGGTGCTCATGTGATTAGCCATATTGCTCCACCAAAGTCATCAAATTTCTTTTCGGATTTTGGAATGGACAGTAGTTTCCCCAAGAGAACTAGTTCAAATACCTCAAAAGTTCAAATTCAGGAAACTGATGAAGCAAGAAAGAAATTCACAAATGCAAAATCTATCTCTTCCGCACAATTTTTTGGTGACCAGAACAGATCTGCTGATGTTGATGCTCAAGCCTCCTTGCAGAAGTTCTCGGGTTCTGCTGCCATCTCCAGTGCTGATCTATTTGGTGACAGGGGAGATCCAGCTATTGATCTTAATGCAAGCGACCTAATCAACCGACTGTCTTTTCAGGCACAACAGGATATCTCTTCCTTAAAGAATATTGCTGGAGAGACGGGGAAGAAGCTTAGCTCGTTGGCATCCAATTTAATGACGGATCTTCAGGACAGAATCCTGTGA

Protein Analysis

408

Amino Acids

43.88

Weight (kDa)

9.05

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 12 - 114 9e-36 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012280)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17890 AT4G17890
fragaria_vesca FvH4_5g22130
malus_domestica MD00G1122000.v1.1 MD00G1183400.v1.1 MD06G1034600.v1.1
prunus_persica Prupe.5G035300_v2.0.a1
pyrus_communis pycom06g02860 pycom16g24390
rosa_chinensis RchiOBHm_Chr7g0209771
rosa_laevigata RLG00000003094
rosa_multiflora Rmu_sc0001818.1_g000012
rosa_roxburghii Rroxscaffold_3G00248980
rosa_rugosa Rorug07G0116100
rosa_samantha Rh7AG249900 Rh7BG243800 Rh7CG265900 Rh7DG255700
rosa_wichuraiana Rw7G021170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 710
AciI CCGC 3 cut(s) 30, 147, 948
AclWI GGATC 4 cut(s) 131, 721, 1058, 1209
AcsI RAATTY 4 cut(s) 196, 820, 891, 920
AcuI CTGAAG 2 cut(s) 675, 1190
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 314, 547, 641
AfiI CCNNNNNNNGG 3 cut(s) 103, 566, 710
AgsI TTSAA 3 cut(s) 19, 873, 890
AhlI ACTAGT 1 cut(s) 866
AluBI AGCT 9 cut(s) 180, 223, 237, 326, 464, 710, 1070, 1171, 1176
AluI AGCT 9 cut(s) 180, 223, 237, 326, 464, 710, 1070, 1171, 1176
Alw21I GWGCWC 3 cut(s) 259, 607, 784
Alw26I GTCTC 3 cut(s) 167, 758, 1151
Alw44I GTGCAC 1 cut(s) 255
AlwI GGATC 4 cut(s) 131, 721, 1058, 1209
Ama87I CYCGRG 1 cut(s) 1015
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 4 cut(s) 274, 326, 509, 1025
ApoI RAATTY 4 cut(s) 196, 820, 891, 920
Asp700I GAANNNNTTC 1 cut(s) 868
AsuC2I CCSGG 1 cut(s) 592
AsuHPI GGTGA 3 cut(s) 393, 974, 1064
AvaI CYCGRG 1 cut(s) 1015
BaeGI GKGCMC 1 cut(s) 259
BauI CACGAG 1 cut(s) 205
BbsI GAAGAC 2 cut(s) 383, 593
Bbv12I GWGCWC 3 cut(s) 259, 607, 784
BbvI GCAGC 4 cut(s) 286, 313, 496, 1012
BccI CCATC 4 cut(s) 276, 284, 403, 1037
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BcnI CCSGG 1 cut(s) 592
BcoDI GTCTC 3 cut(s) 167, 758, 1151
BcuI ACTAGT 1 cut(s) 866
BfaI CTAG 1 cut(s) 867
BfmI CTRYAG 1 cut(s) 480
BfuAI ACCTGC 1 cut(s) 377
BglII AGATCT 2 cut(s) 188, 974
BisI GCNGC 4 cut(s) 275, 327, 510, 1026
BlpI GCTNAGC 1 cut(s) 1172
BlsI GCNGC 4 cut(s) 276, 328, 511, 1027
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 1 cut(s) 592
BmeT110I CYCGRG 1 cut(s) 1015
BmrFI CCNGG 1 cut(s) 592
BmrI ACTGGG 2 cut(s) 591, 665
BmsI GCATC 7 cut(s) 116, 144, 419, 437, 734, 979, 1193
BmuI ACTGGG 2 cut(s) 591, 665
BpiI GAAGAC 2 cut(s) 383, 593
BpmI CTGGAG 2 cut(s) 1018, 1173
Bpu1102I GCTNAGC 1 cut(s) 1172
BpuEI CTTGAG 1 cut(s) 978
BpuMI CCSGG 1 cut(s) 592
Bsa29I ATCGAT 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 591
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 566, 710
Bse1I ACTGG 7 cut(s) 543, 586, 662, 671, 677, 781, 1035
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 591
BseGI GGATG 4 cut(s) 287, 295, 716, 1184
BseLI CCNNNNNNNGG 3 cut(s) 103, 566, 710
BseMII CTCAG 3 cut(s) 215, 428, 753
BseNI ACTGG 7 cut(s) 543, 586, 662, 671, 677, 781, 1035
BseSI GKGCMC 1 cut(s) 259
BseXI GCAGC 4 cut(s) 286, 313, 496, 1012
BshVI ATCGAT 1 cut(s) 138
BsiHKAI GWGCWC 3 cut(s) 259, 607, 784
BsiHKCI CYCGRG 1 cut(s) 1015
BsiSI CCGG 1 cut(s) 591
BslI CCNNNNNNNGG 3 cut(s) 103, 566, 710
BsmAI GTCTC 3 cut(s) 167, 758, 1151
BsmBI CGTCTC 1 cut(s) 1151
BsoBI CYCGRG 1 cut(s) 1015
Bsp1286I GDGCHC 3 cut(s) 259, 607, 784
Bsp143I GATC 9 cut(s) 123, 188, 643, 713, 974, 1042, 1063, 1075, 1201
Bsp1720I GCTNAGC 1 cut(s) 1172
BspACI CCGC 3 cut(s) 30, 147, 948
BspCNI CTCAG 3 cut(s) 216, 427, 754
BspDI ATCGAT 1 cut(s) 138
BspMI ACCTGC 1 cut(s) 377
BspPI GGATC 4 cut(s) 131, 721, 1058, 1209
BspQI GCTCTTC 1 cut(s) 651
BsrI ACTGG 7 cut(s) 543, 586, 662, 671, 677, 781, 1035
BssECI CCNNGG 1 cut(s) 591
BssMI GATC 9 cut(s) 123, 188, 643, 713, 974, 1042, 1063, 1075, 1201
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 205
Bst2BI CACGAG 1 cut(s) 205
Bst4CI ACNGT 4 cut(s) 254, 550, 848, 1107
Bst6I CTCTTC 4 cut(s) 375, 651, 949, 1138
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 141
BstC8I GCNNGC 2 cut(s) 607, 1087
BstDEI CTNAG 6 cut(s) 224, 309, 414, 573, 762, 1172
BstEII GGTNACC 1 cut(s) 962
BstF5I GGATG 4 cut(s) 287, 295, 716, 1184
BstKTI GATC 9 cut(s) 126, 191, 646, 716, 977, 1045, 1066, 1078, 1204
BstMAI GTCTC 3 cut(s) 167, 758, 1151
BstMBI GATC 9 cut(s) 123, 188, 643, 713, 974, 1042, 1063, 1075, 1201
BstMWI GCNNNNNNNGC 2 cut(s) 141, 383
BstPI GGTNACC 1 cut(s) 962
BstSCI CCNGG 1 cut(s) 590
BstSFI CTRYAG 1 cut(s) 480
BstSLI GKGCMC 1 cut(s) 259
BstV1I GCAGC 4 cut(s) 286, 313, 496, 1012
BstV2I GAAGAC 2 cut(s) 383, 593
BstX2I RGATCY 5 cut(s) 123, 188, 974, 1063, 1201
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 5 cut(s) 123, 188, 974, 1063, 1201
Bsu15I ATCGAT 1 cut(s) 138
BsuTUI ATCGAT 1 cut(s) 138
BtsCI GGATG 4 cut(s) 287, 295, 716, 1184
BtsIMutI CAGTG 3 cut(s) 684, 774, 1042
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 2 cut(s) 607, 1087
ClaI ATCGAT 1 cut(s) 138
Csp6I GTAC 3 cut(s) 313, 546, 640
CviAII CATG 3 cut(s) 278, 458, 785
CviQI GTAC 3 cut(s) 313, 546, 640
DdeI CTNAG 6 cut(s) 224, 309, 414, 573, 762, 1172
DpnI GATC 9 cut(s) 125, 190, 645, 715, 976, 1044, 1065, 1077, 1203
DpnII GATC 9 cut(s) 123, 188, 643, 713, 974, 1042, 1063, 1075, 1201
Eam1104I CTCTTC 4 cut(s) 375, 651, 949, 1138
EarI CTCTTC 4 cut(s) 375, 651, 949, 1138
Eco32I GATATC 1 cut(s) 1129
Eco57I CTGAAG 2 cut(s) 675, 1190
Eco88I CYCGRG 1 cut(s) 1015
Eco91I GGTNACC 1 cut(s) 962
EcoO65I GGTNACC 1 cut(s) 962
EcoRV GATATC 1 cut(s) 1129
Esp3I CGTCTC 1 cut(s) 1151
FaeI CATG 3 cut(s) 281, 461, 788
FatI CATG 3 cut(s) 277, 457, 784
FauNDI CATATG 2 cut(s) 118, 535
Fnu4HI GCNGC 4 cut(s) 275, 327, 510, 1026
FokI GGATG 4 cut(s) 294, 302, 703, 1171
Fsp4HI GCNGC 4 cut(s) 275, 327, 510, 1026
FspBI CTAG 1 cut(s) 867
GluI GCNGC 4 cut(s) 275, 327, 510, 1026
GsuI CTGGAG 2 cut(s) 1018, 1173
HapII CCGG 1 cut(s) 591
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 281, 461, 788
HindIII AAGCTT 1 cut(s) 1169
HinfI GANTC 3 cut(s) 203, 211, 1215
HpaII CCGG 1 cut(s) 591
HphI GGTGA 3 cut(s) 393, 974, 1064
Hpy166II GTNNAC 4 cut(s) 172, 210, 257, 315
Hpy188I TCNGA 4 cut(s) 10, 41, 648, 832
Hpy188III TCNNGA 8 cut(s) 215, 321, 420, 443, 451, 729, 896, 1208
Hpy8I GTNNAC 4 cut(s) 172, 210, 257, 315
HpyAV CCTTC 2 cut(s) 49, 693
HpyCH4III ACNGT 4 cut(s) 254, 550, 848, 1107
HpyCH4V TGCA 7 cut(s) 135, 257, 410, 725, 932, 1006, 1085
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 383
HpyF3I CTNAG 6 cut(s) 224, 309, 414, 573, 762, 1172
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 281, 461, 788
Kzo9I GATC 9 cut(s) 123, 188, 643, 713, 974, 1042, 1063, 1075, 1201
LguI GCTCTTC 1 cut(s) 651
LmnI GCTCC 3 cut(s) 149, 469, 808
Lsp1109I GCAGC 4 cut(s) 286, 313, 496, 1012
LweI GCATC 7 cut(s) 116, 144, 419, 437, 734, 979, 1193
MaeI CTAG 1 cut(s) 867
MaeIII GTNAC 4 cut(s) 112, 538, 962, 1052
MalI GATC 9 cut(s) 125, 190, 645, 715, 976, 1044, 1065, 1077, 1203
MboI GATC 9 cut(s) 123, 188, 643, 713, 974, 1042, 1063, 1075, 1201
MflI RGATCY 5 cut(s) 123, 188, 974, 1063, 1201
MhlI GDGCHC 3 cut(s) 259, 607, 784
MluCI AATT 8 cut(s) 196, 300, 747, 820, 891, 920, 953, 1189
MlyI GAGTC 2 cut(s) 197, 205
MmeI TCCRAC 1 cut(s) 728
MnlI CCTC 9 cut(s) 236, 287, 298, 376, 537, 570, 697, 889, 1009
MroXI GAANNNNTTC 1 cut(s) 868
MseI TTAA 5 cut(s) 270, 552, 1080, 1139, 1193
MslI CAYNNNNRTG 1 cut(s) 534
MspA1I CMGCKG 4 cut(s) 32, 149, 223, 710
MspI CCGG 1 cut(s) 591
MspR9I CCNGG 1 cut(s) 592
MwoI GCNNNNNNNGC 2 cut(s) 141, 383
NciI CCSGG 1 cut(s) 592
NdeI CATATG 2 cut(s) 118, 535
NdeII GATC 9 cut(s) 123, 188, 643, 713, 974, 1042, 1063, 1075, 1201
NlaIII CATG 3 cut(s) 281, 461, 788
NmeAIII GCCGAG 2 cut(s) 144, 355
NmuCI GTSAC 2 cut(s) 962, 1052
PciSI GCTCTTC 1 cut(s) 651
PdmI GAANNNNTTC 1 cut(s) 868
PfeI GAWTC 1 cut(s) 1215
PflMI CCANNNNNTGG 1 cut(s) 710
PkrI GCNGC 4 cut(s) 276, 328, 511, 1027
PleI GAGTC 2 cut(s) 197, 205
PpsI GAGTC 2 cut(s) 197, 205
PspEI GGTNACC 1 cut(s) 962
PsuI RGATCY 5 cut(s) 123, 188, 974, 1063, 1201
PvuII CAGCTG 2 cut(s) 223, 710
RsaI GTAC 3 cut(s) 314, 547, 641
RsaNI GTAC 3 cut(s) 313, 546, 640
RseI CAYNNNNRTG 1 cut(s) 534
SapI GCTCTTC 1 cut(s) 651
SaqAI TTAA 5 cut(s) 270, 552, 1080, 1139, 1193
SatI GCNGC 4 cut(s) 275, 327, 510, 1026
Sau3AI GATC 9 cut(s) 123, 188, 643, 713, 974, 1042, 1063, 1075, 1201
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 2 cut(s) 197, 205
ScrFI CCNGG 1 cut(s) 592
SduI GDGCHC 3 cut(s) 259, 607, 784
SfaNI GCATC 7 cut(s) 116, 144, 419, 437, 734, 979, 1193
SfcI CTRYAG 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 534
SmlI CTYRAG 1 cut(s) 993
SmoI CTYRAG 1 cut(s) 993
SpeI ACTAGT 1 cut(s) 866
Sse9I AATT 8 cut(s) 196, 300, 747, 820, 891, 920, 953, 1189
SsiI CCGC 3 cut(s) 30, 147, 948
SspI AATATT 1 cut(s) 1147
SspMI CTAG 1 cut(s) 867
StyD4I CCNGG 1 cut(s) 590
TaaI ACNGT 4 cut(s) 254, 550, 848, 1107
TaqI TCGA 3 cut(s) 78, 138, 730
TasI AATT 8 cut(s) 196, 300, 747, 820, 891, 920, 953, 1189
TatI WGTACW 2 cut(s) 312, 545
TfiI GAWTC 1 cut(s) 1215
Tru1I TTAA 5 cut(s) 270, 552, 1080, 1139, 1193
Tru9I TTAA 5 cut(s) 270, 552, 1080, 1139, 1193
TscAI CASTG 3 cut(s) 684, 781, 1042
TseFI GTSAC 2 cut(s) 962, 1052
TseI GCWGC 4 cut(s) 274, 326, 509, 1025
Tsp45I GTSAC 2 cut(s) 962, 1052
TspDTI ATGAA 3 cut(s) 143, 474, 921
TspGWI ACGGA 2 cut(s) 302, 1214
TspRI CASTG 3 cut(s) 684, 781, 1042
Van91I CCANNNNNTGG 1 cut(s) 710
VneI GTGCAC 1 cut(s) 255
XapI RAATTY 4 cut(s) 196, 820, 891, 920
XmnI GAANNNNTTC 1 cut(s) 868
XspI CTAG 1 cut(s) 867
ZrmI AGTACT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.