FvH4_5g27121

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011715
Physical Location & Seq
Reverse (-)
18402638 .. 18406708
4071 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27121.t2

Sequence Viewer

Length: 1491 bp
ATGACAGAAGAAGAACTTAACAGCTCTTTAGAGTATACTCCAACATGGGTGGTAGCAGGTGTATGCTTTGTCATTGTTCTGTTATCTCTTTGTGTCGAGCGTGGCCTTCATAAACTTGGAAAGTGCTTGCAGCATGAGAGACAAGATGCACTATATGAAGCCTTACAAAAACTAAAAGAAGAATTAATGCTTTTAGGGTTCATTTCCCTTCTGCTAACTGTGTCTCAACGTTCCATAAGCCGCATATGCATCCCTGCCCATGTCGCAACTCACATGTTGCCATGTAAGAGGGAAACTGCTGAGCACAATACCCCTGCACATTACTTTCTTAATCAAGCTACAAACAATGGCCGACGCCTTCTGTCAGAAGCAACTTCAGATCACTGTCAGCTCAAGGGGAAGGTTCCGTTGTTATCATTGGAAGGATTACATCATCTACACATCTTCATCTTTGTATTAGCAGTTGTTCATGTGATCTTCTGTGTCTCAACTATGGTTCTTGGAGGGGCAAGAATTCGACAATGGAAGCGCTGGGAGGATTCCATCCAGAAGGATCGAAGAAGTGGCGTTGCTCACGTCAGTCATCACCATCATGAGTTCTTGAAAGAACGTGCAGATGGATATTGGAGAAGAGCAGCTATTATAGGTTGGATGATAGCATTCTGCAAACAATTTTATGGCTCCGTCACCAAGTCAGATTACATTGCACTGCGGCAAGGGTTTATCAAGGAGCATTGCCCTGGAAATCCTAATTTTGATTTTCACAAATACATGATGCGGACACTTGAGATTGATTTTAGAAAGATTGTTGGGATAAGCTGGTACCTGTGGCTCTTTGTTGTGCTGTTTTTGTTGCTGAATGTGGATGGATGGAACACATACTTCTGGTTAGCATTTTTGCCGCTGATTCTTCTGCTTCTAGTGGGGGCCAAACTAGAACACATAATTAGCCGCTTGGCTCAGGATGTTACACAATCAGGAGTACATGCTAATGAAGCAGCTACAAGAGTGAGGCCTTCTGATGAACACTTCTGGTTCAGAAATCCTAAAATTGTTCTCATTTTGATCCACTTCATTCTGTTTCAAAATTCATTCGAGATTGCATTCTTCTTCTGGATTTGGACCACCTATGGATTTCACTCATGCATCATGGAAAAGTTGGGCTACATCATCGCCAGACTTATTATGGGTTTGATAGTTCAAGTGCTTTGCAGTTATAGCACCCTACCATTGTATGCAATCGTCACTCAGATGGGTAGCATGTTCAAGGAAGGAATGTTCGATCAAATGGTGCAGAGTGCCATCGGTCTGTGGGCTGGAGATGTAATCAGTCGACGTGAAGGCAGCGGGCGATCCTCCTCAGGCAGTGTGCACAAAATGATCAATAATGAATCGACCCAAACTGTCGATGTGGCAGAACAAGAAATGGTTGCCATGGCTAACACTATAGATGTGCTCCCTTGTCATGTTCAGCCACCTTCATCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

497

Amino Acids

56.78

Weight (kDa)

8.21

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 418 1.3e-168 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011715)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24250 AT4G24250
fragaria_vesca FvH4_3g13171 FvH4_5g27121 FvH4_5g27121
malus_domestica MD08G1246900.v1.1 MD08G1251000.v1.1
prunus_persica Prupe.1G588000_v2.0.a1
pyrus_communis pycom08g21400 pycom08g21750
rosa_chinensis RchiOBHm_Chr7g0218121
rosa_laevigata RLG00000002398
rosa_multiflora Rmu_sc0003627.1_g000013
rosa_roxburghii Rroxscaffold_3G00241290
rosa_rugosa Rorug07G0174300
rosa_samantha Rh7AG316700 Rh7BG307800 Rh7CG333600 Rh7DG315600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 47
Acc65I GGTACC 1 cut(s) 822
AccB1I GGYRCC 1 cut(s) 822
AccI GTMKAC 2 cut(s) 35, 1333
AciI CCGC 6 cut(s) 241, 712, 778, 902, 952, 1347
AclI AACGTT 1 cut(s) 229
AclWI GGATC 3 cut(s) 561, 1060, 1347
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 2 cut(s) 513, 1087
AcuI CTGAAG 1 cut(s) 360
AcyI GRCGYC 1 cut(s) 355
AfaI GTAC 2 cut(s) 824, 984
AfeI AGCGCT 1 cut(s) 530
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 4 cut(s) 604, 1085, 1202, 1267
AjiI CACGTC 2 cut(s) 577, 1337
AjnI CCWGG 1 cut(s) 739
AloI GAACNNNNNNTCC 2 cut(s) 1262, 1294
AluBI AGCT 6 cut(s) 24, 338, 391, 638, 819, 1001
AluI AGCT 6 cut(s) 24, 338, 391, 638, 819, 1001
Alw21I GWGCWC 3 cut(s) 306, 1374, 1458
Alw26I GTCTC 3 cut(s) 133, 228, 490
Alw44I GTGCAC 1 cut(s) 1370
AlwI GGATC 3 cut(s) 561, 1060, 1347
Aor51HI AGCGCT 1 cut(s) 530
AoxI GGCC 4 cut(s) 103, 349, 927, 1013
ApaLI GTGCAC 1 cut(s) 1370
ApeKI GCWGC 4 cut(s) 130, 635, 998, 1344
ApoI RAATTY 2 cut(s) 513, 1087
AseI ATTAAT 1 cut(s) 185
Asp718I GGTACC 1 cut(s) 822
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 2 cut(s) 927, 1122
AsuHPI GGTGA 2 cut(s) 578, 679
AvaII GGWCC 1 cut(s) 1122
AxyI CCTNAGG 1 cut(s) 1360
BaeGI GKGCMC 1 cut(s) 1374
BanI GGYRCC 1 cut(s) 822
Bbv12I GWGCWC 3 cut(s) 306, 1374, 1458
BbvI GCAGC 4 cut(s) 142, 647, 1010, 1356
BccI CCATC 7 cut(s) 551, 597, 611, 860, 864, 1246, 1310
BciT130I CCWGG 1 cut(s) 741
BclI TGATCA 1 cut(s) 1380
BcoDI GTCTC 3 cut(s) 133, 228, 490
BfaI CTAG 2 cut(s) 920, 935
BfmI CTRYAG 1 cut(s) 1446
BfoI RGCGCY 1 cut(s) 532
BfuAI ACCTGC 1 cut(s) 47
BisI GCNGC 8 cut(s) 131, 241, 636, 713, 902, 952, 999, 1345
BlpI GCTNAGC 1 cut(s) 300
BlsI GCNGC 8 cut(s) 132, 242, 637, 714, 903, 953, 1000, 1346
Bme1390I CCNGG 1 cut(s) 741
Bme18I GGWCC 1 cut(s) 1122
BmgBI CACGTC 2 cut(s) 577, 1337
BmgT120I GGNCC 2 cut(s) 927, 1122
BmiI GGNNCC 4 cut(s) 405, 682, 824, 928
BmrFI CCNGG 1 cut(s) 741
BmsI GCATC 4 cut(s) 136, 258, 765, 1155
BpmI CTGGAG 1 cut(s) 1338
Bpu10I CCTNAGC 1 cut(s) 960
Bpu1102I GCTNAGC 1 cut(s) 300
BpuEI CTTGAG 2 cut(s) 377, 806
BsaHI GRCGYC 1 cut(s) 355
BsaJI CCNNGG 2 cut(s) 739, 1434
Bse21I CCTNAGG 1 cut(s) 1360
Bse3DI GCAATG 2 cut(s) 702, 733
BseBI CCWGG 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 739, 1434
BseGI GGATG 7 cut(s) 249, 543, 657, 871, 875, 970, 1481
BseMI GCAATG 2 cut(s) 702, 733
BseMII CTCAG 4 cut(s) 291, 974, 1262, 1374
BseRI GAGGAG 1 cut(s) 1348
BseSI GKGCMC 1 cut(s) 1374
BseXI GCAGC 4 cut(s) 142, 647, 1010, 1356
BseYI CCCAGC 1 cut(s) 531
BsgI GTGCAG 3 cut(s) 300, 633, 1313
BshFI GGCC 4 cut(s) 105, 351, 929, 1015
BshNI GGYRCC 1 cut(s) 822
BsiHKAI GWGCWC 3 cut(s) 306, 1374, 1458
BsmAI GTCTC 3 cut(s) 133, 228, 490
BsmI GAATGC 2 cut(s) 659, 1103
BsnI GGCC 4 cut(s) 105, 351, 929, 1015
Bsp1286I GDGCHC 3 cut(s) 306, 1374, 1458
Bsp143I GATC 7 cut(s) 379, 474, 553, 1065, 1282, 1352, 1380
Bsp1720I GCTNAGC 1 cut(s) 300
Bsp19I CCATGG 1 cut(s) 1434
BspACI CCGC 6 cut(s) 241, 712, 778, 902, 952, 1347
BspANI GGCC 4 cut(s) 105, 351, 929, 1015
BspCNI CTCAG 4 cut(s) 292, 973, 1261, 1373
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 4 cut(s) 405, 682, 824, 928
BspMI ACCTGC 1 cut(s) 47
BspPI GGATC 3 cut(s) 561, 1060, 1347
BspQI GCTCTTC 1 cut(s) 625
BspT107I GGYRCC 1 cut(s) 822
BsrDI GCAATG 2 cut(s) 702, 733
BssECI CCNNGG 2 cut(s) 739, 1434
BssMI GATC 7 cut(s) 379, 474, 553, 1065, 1282, 1352, 1380
BssNAI GTATAC 1 cut(s) 36
BssNI GRCGYC 1 cut(s) 355
BssT1I CCWWGG 1 cut(s) 1434
Bst1107I GTATAC 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 3 cut(s) 220, 386, 1405
Bst6I CTCTTC 1 cut(s) 625
BstACI GRCGYC 1 cut(s) 355
BstC8I GCNNGC 2 cut(s) 128, 1349
BstDEI CTNAG 5 cut(s) 300, 960, 1248, 1360, 1488
BstDSI CCRYGG 1 cut(s) 1434
BstF5I GGATG 7 cut(s) 249, 543, 657, 871, 875, 970, 1481
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 7 cut(s) 382, 477, 556, 1068, 1285, 1355, 1383
BstMAI GTCTC 3 cut(s) 133, 228, 490
BstMBI GATC 7 cut(s) 379, 474, 553, 1065, 1282, 1352, 1380
BstMWI GCNNNNNNNGC 4 cut(s) 246, 263, 995, 1218
BstNI CCWGG 1 cut(s) 741
BstNSI RCATGY 3 cut(s) 277, 989, 1264
BstSCI CCNGG 1 cut(s) 739
BstSFI CTRYAG 1 cut(s) 1446
BstSLI GKGCMC 1 cut(s) 1374
BstV1I GCAGC 4 cut(s) 142, 647, 1010, 1356
BstZ17I GTATAC 1 cut(s) 36
Bsu36I CCTNAGG 1 cut(s) 1360
BsuRI GGCC 4 cut(s) 105, 351, 929, 1015
BtgI CCRYGG 1 cut(s) 1434
BtgZI GCGATG 1 cut(s) 1156
BtrI CACGTC 2 cut(s) 577, 1337
BtsCI GGATG 7 cut(s) 249, 543, 657, 871, 875, 970, 1481
BtsI GCAGTG 2 cut(s) 707, 1372
BtsIMutI CAGTG 3 cut(s) 382, 707, 1372
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 2 cut(s) 128, 1349
CciI TCATGA 1 cut(s) 592
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 2 cut(s) 927, 1122
CseI GACGC 1 cut(s) 363
Csp6I GTAC 2 cut(s) 823, 983
CspCI CAANNNNNGTGG 2 cut(s) 30, 65
CviQI GTAC 2 cut(s) 823, 983
DdeI CTNAG 5 cut(s) 300, 960, 1248, 1360, 1488
DpnI GATC 7 cut(s) 381, 476, 555, 1067, 1284, 1354, 1382
DpnII GATC 7 cut(s) 379, 474, 553, 1065, 1282, 1352, 1380
EaeI YGGCCR 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 625
EarI CTCTTC 1 cut(s) 625
Eco130I CCWWGG 1 cut(s) 1434
Eco147I AGGCCT 1 cut(s) 1015
Eco47I GGWCC 1 cut(s) 1122
Eco47III AGCGCT 1 cut(s) 530
Eco57I CTGAAG 1 cut(s) 360
Eco81I CCTNAGG 1 cut(s) 1360
EcoRI GAATTC 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 739
EcoT14I CCWWGG 1 cut(s) 1434
EcoT22I ATGCAT 2 cut(s) 251, 1148
ErhI CCWWGG 1 cut(s) 1434
FalI AAGNNNNNCTT 1 cut(s) 32
FauI CCCGC 1 cut(s) 1340
FauNDI CATATG 1 cut(s) 245
FbaI TGATCA 1 cut(s) 1380
FblI GTMKAC 2 cut(s) 35, 1333
Fnu4HI GCNGC 8 cut(s) 131, 241, 636, 713, 902, 952, 999, 1345
FokI GGATG 7 cut(s) 236, 530, 664, 878, 882, 977, 1468
Fsp4HI GCNGC 8 cut(s) 131, 241, 636, 713, 902, 952, 999, 1345
FspBI CTAG 2 cut(s) 920, 935
GlaI GCGC 1 cut(s) 530
GluI GCNGC 8 cut(s) 131, 241, 636, 713, 902, 952, 999, 1345
GsaI CCCAGC 1 cut(s) 535
GsuI CTGGAG 1 cut(s) 1338
HaeII RGCGCY 1 cut(s) 532
HaeIII GGCC 4 cut(s) 105, 351, 929, 1015
HgaI GACGC 1 cut(s) 363
HhaI GCGC 1 cut(s) 531
Hin1I GRCGYC 1 cut(s) 355
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 1 cut(s) 1334
HindII GTYRAC 1 cut(s) 1334
HinfI GANTC 3 cut(s) 539, 907, 1391
HphI GGTGA 2 cut(s) 578, 679
Hpy166II GTNNAC 3 cut(s) 36, 1334, 1372
Hpy188I TCNGA 6 cut(s) 367, 379, 697, 1021, 1040, 1251
Hpy188III TCNNGA 7 cut(s) 547, 593, 601, 962, 978, 1096, 1114
Hpy8I GTNNAC 3 cut(s) 36, 1334, 1372
Hpy99I CGWCG 2 cut(s) 357, 1338
HpyCH4III ACNGT 3 cut(s) 220, 386, 1405
HpyCH4IV ACGT 4 cut(s) 229, 576, 610, 1336
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 263, 995, 1218
HpyF3I CTNAG 5 cut(s) 300, 960, 1248, 1360, 1488
HpySE526I ACGT 4 cut(s) 229, 576, 610, 1336
Hsp92I GRCGYC 1 cut(s) 355
HspAI GCGC 1 cut(s) 529
KpnI GGTACC 1 cut(s) 826
Ksp22I TGATCA 1 cut(s) 1380
Kzo9I GATC 7 cut(s) 379, 474, 553, 1065, 1282, 1352, 1380
LguI GCTCTTC 1 cut(s) 625
LmnI GCTCC 3 cut(s) 686, 730, 1461
Lsp1109I GCAGC 4 cut(s) 142, 647, 1010, 1356
LweI GCATC 4 cut(s) 136, 258, 765, 1155
MaeI CTAG 2 cut(s) 920, 935
MaeII ACGT 4 cut(s) 229, 576, 610, 1336
MaeIII GTNAC 3 cut(s) 685, 967, 1243
MalI GATC 7 cut(s) 381, 476, 555, 1067, 1284, 1354, 1382
MboI GATC 7 cut(s) 379, 474, 553, 1065, 1282, 1352, 1380
MhlI GDGCHC 3 cut(s) 306, 1374, 1458
MluCI AATT 7 cut(s) 182, 513, 671, 751, 945, 1050, 1087
MmeI TCCRAC 2 cut(s) 65, 629
MnlI CCTC 6 cut(s) 282, 497, 529, 1005, 1366, 1369
Mph1103I ATGCAT 2 cut(s) 251, 1148
MseI TTAA 3 cut(s) 18, 185, 330
MslI CAYNNNNRTG 3 cut(s) 591, 990, 1250
MspA1I CMGCKG 2 cut(s) 904, 1347
MspR9I CCNGG 1 cut(s) 741
Mva1269I GAATGC 2 cut(s) 659, 1103
MvaI CCWGG 1 cut(s) 741
MwoI GCNNNNNNNGC 4 cut(s) 246, 263, 995, 1218
NcoI CCATGG 1 cut(s) 1434
NdeI CATATG 1 cut(s) 245
NdeII GATC 7 cut(s) 379, 474, 553, 1065, 1282, 1352, 1380
NlaIV GGNNCC 4 cut(s) 405, 682, 824, 928
NmuCI GTSAC 2 cut(s) 685, 1243
NsiI ATGCAT 2 cut(s) 251, 1148
NspI RCATGY 3 cut(s) 277, 989, 1264
PagI TCATGA 1 cut(s) 592
PaqCI CACCTGC 1 cut(s) 47
PceI AGGCCT 1 cut(s) 1015
PciI ACATGT 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 625
PctI GAATGC 2 cut(s) 659, 1103
PfeI GAWTC 3 cut(s) 539, 907, 1391
PkrI GCNGC 8 cut(s) 132, 242, 637, 714, 903, 953, 1000, 1346
PscI ACATGT 1 cut(s) 273
PshBI ATTAAT 1 cut(s) 185
Psp1406I AACGTT 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 531
PspGI CCWGG 1 cut(s) 739
PspN4I GGNNCC 4 cut(s) 405, 682, 824, 928
PspPI GGNCC 2 cut(s) 927, 1122
RsaI GTAC 2 cut(s) 824, 984
RsaNI GTAC 2 cut(s) 823, 983
RseI CAYNNNNRTG 3 cut(s) 591, 990, 1250
SalI GTCGAC 1 cut(s) 1332
SapI GCTCTTC 1 cut(s) 625
SaqAI TTAA 3 cut(s) 18, 185, 330
SatI GCNGC 8 cut(s) 131, 241, 636, 713, 902, 952, 999, 1345
Sau3AI GATC 7 cut(s) 379, 474, 553, 1065, 1282, 1352, 1380
Sau96I GGNCC 2 cut(s) 927, 1122
ScrFI CCNGG 1 cut(s) 741
SduI GDGCHC 3 cut(s) 306, 1374, 1458
SfaNI GCATC 4 cut(s) 136, 258, 765, 1155
SfcI CTRYAG 1 cut(s) 1446
SinI GGWCC 1 cut(s) 1122
SmiMI CAYNNNNRTG 3 cut(s) 591, 990, 1250
SmlI CTYRAG 2 cut(s) 392, 785
SmoI CTYRAG 2 cut(s) 392, 785
Sse9I AATT 7 cut(s) 182, 513, 671, 751, 945, 1050, 1087
SseBI AGGCCT 1 cut(s) 1015
SsiI CCGC 6 cut(s) 241, 712, 778, 902, 952, 1347
SspMI CTAG 2 cut(s) 920, 935
StuI AGGCCT 1 cut(s) 1015
StyD4I CCNGG 1 cut(s) 739
StyI CCWWGG 1 cut(s) 1434
TaaI ACNGT 3 cut(s) 220, 386, 1405
TaiI ACGT 4 cut(s) 232, 579, 613, 1339
TaqI TCGA 8 cut(s) 96, 517, 556, 1095, 1281, 1333, 1394, 1407
TaqII GACCGA 1 cut(s) 1295
TasI AATT 7 cut(s) 182, 513, 671, 751, 945, 1050, 1087
TatI WGTACW 1 cut(s) 982
TauI GCSGC 4 cut(s) 243, 715, 904, 954
TfiI GAWTC 3 cut(s) 539, 907, 1391
Tru1I TTAA 3 cut(s) 18, 185, 330
Tru9I TTAA 3 cut(s) 18, 185, 330
TscAI CASTG 3 cut(s) 389, 714, 1372
TseFI GTSAC 2 cut(s) 685, 1243
TseI GCWGC 4 cut(s) 130, 635, 998, 1344
Tsp45I GTSAC 2 cut(s) 685, 1243
TspGWI ACGGA 2 cut(s) 396, 673
TspRI CASTG 3 cut(s) 389, 714, 1372
VneI GTGCAC 1 cut(s) 1370
VpaK11BI GGWCC 1 cut(s) 1122
VspI ATTAAT 1 cut(s) 185
XapI RAATTY 2 cut(s) 513, 1087
XceI RCATGY 3 cut(s) 277, 989, 1264
XcmI CCANNNNNNNNNTGG 1 cut(s) 1183
XmiI GTMKAC 2 cut(s) 35, 1333
XspI CTAG 2 cut(s) 920, 935
Zsp2I ATGCAT 2 cut(s) 251, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.