Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0003400

Physical Location & Seq

Genomic Coordinates Reverse (-)

18417434 .. 18423288

Gene Structure 5855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g27150.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGGATAAAAGTTCTGTCCATAAGCCCCTTCTTTCGAGCCGAGGTTCTGGGCATGCTTTTGTTCGGAAGACGCCTGGTTTTGTGAACTTGGCAAAATGGATTCTGAAGATTGCAATGTGGGTGCTTTTTACTTCATGGGTTGCTCTAATGTTTGTTTTTCCAGCAGAATTTGGGTCTAAACTATTCCAAAAATGGGTTGCAGCAACCAGTGGAACTGTTTTCGGGGTTTCAGGAAGCCTGTTCCTGCTATTCAGTGGTCCCATCCTCATCATTGCTTTCCTTGCTGTAGTGTTTCTTATTATCTCCGGGGAAGAGGAGCTCCATGAGAAGAAGACTTCAAAATATCCAAGTTTTCGCTTATGGACATTCCCAGTTCTTGTGGATGGACCATTTGGCGTGGTTTCTGCTGCTGAGGTTATTGGGATACTACTCTTCATTGTGTATGTTCTCTGGGCTGTCGGTTCTTATACTGCGCGTCTTCTTGGCTCGTTATCTGGTGACTTGACTTTTAAAATGAAAAGTCTTTTGTTCCTGGAAATTTTGGGACTTCGTTTCGGCTCAATTGGCTTGTTTTGCCTGGTTTTTTTGTTCCTGCCGATCGCTAGGGGATCAGTTCTTCTTCGCCTCATAAATATCCCTTTTGAGCATGCTACAAAATATCATGTATGGCTAGGACACCTAACAATGCTGCTCTTCACTGTCCACGGCCTATTATACCTAGTGGCATGGACGATTGAGGGTCGCCTCCTTCAGCAAATATTGGAGTGGAAAGACATTGGTGTTGCCAATCTTCCCGGAGTAATCAGCCTTCTTGCTGGACTGTTGATGTGGATGACATCATTTGCTCCAGTGAGAAAGCTGAATTTTGAATTATTCTTCTACACTCATCAGTTATATGTCATCTTTGTGGTCTTTTTGGCTTTGCACGTTGGTGACTTCCTTTTTGCAATCGCTGCTGGTGGCATATTTATTTTTCTTCTGGATCGCTTTCTAAGATTCTGTCAATCTAGAAGAGTTGTGGACATAATTTCAGCAAAGTGCCTTCCATGTGGAACCGTAGAATTGGTTCTCGCCAAACCTGAAAATCTGCGGTACAATGCTCTCAGTTTTGTTTTTCTTCAAGTTCGGGAACTATCATGGCTGCAGTGGCATCCTTTCAGTGTATCATCCAGTCCTCTGGATGGTAAATATCATATATCTGTACTTATAAAGGTTCTTGGGAAATGGACAGAAAACCTAAGAGGAAATATCTTAAAAAGCTCGAAGGATGTGCCATGCAGTCTTCCTACTAAGTTAACAGCTTCTGTTGAAGGGCCTTATGGGCATGAGATACCGTACCACATGATGTATGAAAATCTTATTTTAGTAGCTGGTGGCATTGGGATTTCCCCTTTCCTGGCCATCTTGAGTGATATTCTCCATTCTGTTAGGCAAGGAAAGCCTTGTCTACCGAGAAATATATTGATCGTATGGGCGGTTAAAAGATCAGATGAGCTTCCTCTTCTCTCAACCATTGACATGGAGTCAACTTGTCCATCTTTCTCTAATAAACTAAATCTTCAGACTCATGTTTACGTCACTCGGGAATCTGAACCTCCATTGGAAGAGGGTAAAATTCAAAGCACCGTCGAGTCTTCTCTCTGTCCCATGTCCAAAGGGTCTGGTATCTCTGTTTTGGTTGGTACTGGACATAATACATGGTCTGGCCTATACATTATCTCATCCACAGCAGGCTTTGTTTTCCTAATTGCTTTGTTAGATGTCTTCTACATAAACCCTTTTCATATATCCACATGGTGGTACAAAGGGCTCCTTTTCATAGCCTGTATGGCTGCGAGTGTATTTGGCTTTGGGGGTTTTGTGGTTGGCTTATGGCATCTCTGGGACAGAAAAATGTCAGCAAAGGAGCAAAGCAAGGATGATAGGTTAGATGTTAACAAGGCAGAGGATACAAAATCTGTTGCTCACAAGGATTTAGACGAGAGAAGTCTTGCAAACTCAACCAGCATTCATTATGGTGCAAGACCAGACTTCAATGAAATTTTTGGAACGGTATCAAAGGATTGGGGCCATGTTGATATTGGTGTATTTGTTTGTGGTCCTCCGTCTCTTCAATCAAGTGTTGCTAAAGAAATCAGGTCACACAGTGTTAGGAGACAATCCATTGATCCTATCTTCCATTTCAACAGCCATAGCTTTGACCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000293 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006091 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009767 GO:0009987 GO:0015979 GO:0016020 GO:0016491 GO:0016722 GO:0016723 GO:0019684 GO:0022900 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0055114 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NAD_binding_6

Protein Analysis

736

Amino Acids

82.1

Weight (kDa)

8.87

Isoelectric Point (pI)

43.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ferric_reduct	PF01794	173 - 278	6e-19	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	313 - 414	6.6e-20	FAD-binding domain
NAD_binding_6	PF08030	422 - 506	1.5e-10	Ferric reductase NAD binding domain
NAD_binding_6	PF08030	608 - 682	3.4e-07	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49730 AT5G49730 AT5G49740 AT5G49740 AT5G49740
fragaria_vesca	FvH4_5g27150 FvH4_5g27150 FvH4_5g27150 FvH4_5g27150 FvH4_5g27150
malus_domestica	MD08G1250800.v1.1 MD15G1140500.v1.1
prunus_persica	Prupe.1G587800_v2.0.a1 Prupe.1G587800_v2.0.a1
pyrus_communis	pycom08g21770 pycom15g12700
rosa_chinensis	RchiOBHm_Chr7g0218161 RchiOBHm_Chr7g0218171
rosa_laevigata	RLG00000002392
rosa_multiflora	Rmu_sc0003627.1_g000006 Rmu_sc0003627.1_g000007
rosa_roxburghii	Rroxscaffold_3G00241250 Rroxscaffold_3G00241260
rosa_rugosa	Rorug07G0174700 Rorug07G0174700 Rorug07G0174800
rosa_samantha	Rh7AG317100 Rh7AG317200 Rh7BG308300 Rh7BG315800 Rh7CG333900 Rh7CG334000 Rh7DG316000 Rh7DG316200
rosa_wichuraiana	Rw7G026830 Rw7G026840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1208
AccB7I	CCANNNNNTGG	1 cut(s)	1797
AccI	GTMKAC	1 cut(s)	1447
AccII	CGCG	1 cut(s)	475
AciI	CCGC	2 cut(s)	1090, 1475
AclWI	GGATC	3 cut(s)	616, 990, 2162
AcoI	YGGCCR	1 cut(s)	1398
AcsI	RAATTY	5 cut(s)	167, 537, 862, 1614, 2040
AcuI	CTGAAG	3 cut(s)	125, 734, 1544
AcyI	GRCGYC	1 cut(s)	71
AdeI	CACNNNGTG	2 cut(s)	1797, 2147
AfaI	GTAC	5 cut(s)	1094, 1203, 1337, 1684, 1802
AfiI	CCNNNNNNNGG	4 cut(s)	193, 1181, 1396, 1797
AgsI	TTSAA	8 cut(s)	339, 869, 1121, 1310, 1619, 2035, 2114, 2185
AhdI	GACNNNNNGTC	1 cut(s)	1522
AjnI	CCWGG	4 cut(s)	73, 531, 576, 1395
AleI	CACNNNNGTG	1 cut(s)	930
AluBI	AGCT	7 cut(s)	319, 859, 1260, 1301, 1370, 1495, 2196
AluI	AGCT	7 cut(s)	319, 859, 1260, 1301, 1370, 1495, 2196
Alw21I	GWGCWC	1 cut(s)	321
Alw26I	GTCTC	2 cut(s)	2112, 2149
AlwI	GGATC	3 cut(s)	616, 990, 2162
AlwNI	CAGNNNCTG	1 cut(s)	1304
Ama87I	CYCGRG	1 cut(s)	1581
AoxI	GGCC	5 cut(s)	706, 1313, 1398, 1705, 2068
ApeKI	GCWGC	6 cut(s)	200, 407, 688, 953, 1141, 1832
ApoI	RAATTY	5 cut(s)	167, 537, 862, 1614, 2040
ArsI	GACNNNNNNTTYG	2 cut(s)	926, 958
Asp700I	GAANNNNTTC	1 cut(s)	1065
AspLEI	GCGC	1 cut(s)	475
AspS9I	GGNCC	5 cut(s)	257, 386, 1313, 2068, 2099
AsuC2I	CCSGG	2 cut(s)	307, 795
AsuHPI	GGTGA	2 cut(s)	509, 944
AvaI	CYCGRG	1 cut(s)	1581
AvaII	GGWCC	3 cut(s)	257, 386, 2099
BalI	TGGCCA	1 cut(s)	1400
BanII	GRGCYC	2 cut(s)	321, 1812
BarI	GAAGNNNNNNTAC	2 cut(s)	792, 824
BbsI	GAAGAC	6 cut(s)	74, 338, 470, 1274, 1626, 1756
Bbv12I	GWGCWC	1 cut(s)	321
BbvCI	CCTCAGC	1 cut(s)	411
BbvI	GCAGC	6 cut(s)	212, 394, 675, 940, 1128, 1819
BccI	CCATC	5 cut(s)	269, 377, 1175, 1409, 1543
BceAI	ACGGC	1 cut(s)	721
BcgI	CGANNNNNNTGC	2 cut(s)	1252, 1286
BciT130I	CCWGG	4 cut(s)	75, 533, 578, 1397
BciVI	GTATCC	2 cut(s)	417, 1942
BcnI	CCSGG	2 cut(s)	307, 795
BcoDI	GTCTC	2 cut(s)	2112, 2149
BfaI	CTAG	4 cut(s)	603, 671, 719, 1008
BfmI	CTRYAG	3 cut(s)	285, 1142, 2204
BfuI	GTATCC	2 cut(s)	417, 1942
BglI	GCCNNNNNGGC	2 cut(s)	1321, 1829
BisI	GCNGC	6 cut(s)	201, 408, 689, 954, 1142, 1833
BlsI	GCNGC	6 cut(s)	202, 409, 690, 955, 1143, 1834
Bme1390I	CCNGG	6 cut(s)	75, 307, 533, 578, 795, 1397
Bme18I	GGWCC	3 cut(s)	257, 386, 2099
BmeRI	GACNNNNNGTC	1 cut(s)	1522
BmeT110I	CYCGRG	1 cut(s)	1581
BmgT120I	GGNCC	5 cut(s)	257, 386, 1313, 2068, 2099
BmiI	GGNNCC	4 cut(s)	259, 1054, 1811, 2069
BmrFI	CCNGG	6 cut(s)	75, 307, 533, 578, 795, 1397
BmrI	ACTGGG	1 cut(s)	365
BmsI	GCATC	2 cut(s)	1159, 1885
BmuI	ACTGGG	1 cut(s)	365
BpiI	GAAGAC	6 cut(s)	74, 338, 470, 1274, 1626, 1756
BpmI	CTGGAG	1 cut(s)	831
Bpu10I	CCTNAGC	1 cut(s)	411
BpuEI	CTTGAG	1 cut(s)	1426
BpuMI	CCSGG	2 cut(s)	307, 795
BsaHI	GRCGYC	1 cut(s)	71
BsaJI	CCNNGG	3 cut(s)	40, 306, 703
Bsc4I	CCNNNNNNNGG	4 cut(s)	193, 1181, 1396, 1797
Bse1I	ACTGG	5 cut(s)	207, 371, 848, 1170, 1690
Bse3DI	GCAATG	2 cut(s)	120, 270
BseBI	CCWGG	4 cut(s)	75, 533, 578, 1397
BseDI	CCNNGG	3 cut(s)	40, 306, 703
BseGI	GGATG	9 cut(s)	261, 388, 837, 1150, 1166, 1186, 1273, 1721, 1924
BseLI	CCNNNNNNNGG	4 cut(s)	193, 1181, 1396, 1797
BseMI	GCAATG	2 cut(s)	120, 270
BseMII	CTCAG	2 cut(s)	402, 1117
BseNI	ACTGG	5 cut(s)	207, 371, 848, 1170, 1690
BseRI	GAGGAG	1 cut(s)	329
BseXI	GCAGC	6 cut(s)	212, 394, 675, 940, 1128, 1819
Bsh1236I	CGCG	1 cut(s)	475
Bsh1285I	CGRYCG	1 cut(s)	600
BshFI	GGCC	5 cut(s)	708, 1315, 1400, 1707, 2070
BsiEI	CGRYCG	1 cut(s)	600
BsiHKAI	GWGCWC	1 cut(s)	321
BsiHKCI	CYCGRG	1 cut(s)	1581
BsiSI	CCGG	2 cut(s)	306, 795
BslFI	GGGAC	4 cut(s)	243, 558, 1629, 1898
BslI	CCNNNNNNNGG	4 cut(s)	193, 1181, 1396, 1797
BsmAI	GTCTC	2 cut(s)	2112, 2149
BsmBI	CGTCTC	1 cut(s)	2112
BsmFI	GGGAC	4 cut(s)	243, 558, 1629, 1898
BsmI	GAATGC	1 cut(s)	2007
BsnI	GGCC	5 cut(s)	708, 1315, 1400, 1707, 2070
BsoBI	CYCGRG	1 cut(s)	1581
Bsp1286I	GDGCHC	2 cut(s)	321, 1812
Bsp143I	GATC	6 cut(s)	597, 608, 982, 1464, 1484, 2167
BspACI	CCGC	2 cut(s)	1090, 1475
BspANI	GGCC	5 cut(s)	708, 1315, 1400, 1707, 2070
BspCNI	CTCAG	2 cut(s)	403, 1116
BspFNI	CGCG	1 cut(s)	475
BspLI	GGNNCC	4 cut(s)	259, 1054, 1811, 2069
BspMAI	CTGCAG	1 cut(s)	1146
BspPI	GGATC	3 cut(s)	616, 990, 2162
BspQI	GCTCTTC	1 cut(s)	698
BsrDI	GCAATG	2 cut(s)	120, 270
BsrI	ACTGG	5 cut(s)	207, 371, 848, 1170, 1690
BssECI	CCNNGG	3 cut(s)	40, 306, 703
BssMI	GATC	6 cut(s)	597, 608, 982, 1464, 1484, 2167
BssNI	GRCGYC	1 cut(s)	71
Bst2UI	CCWGG	4 cut(s)	75, 533, 578, 1397
Bst4CI	ACNGT	8 cut(s)	217, 700, 822, 1057, 1335, 1627, 2053, 2147
Bst6I	CTCTTC	7 cut(s)	306, 437, 698, 1006, 1506, 1599, 2115
BstACI	GRCGYC	1 cut(s)	71
BstAPI	GCANNNNNTGC	1 cut(s)	953
BstC8I	GCNNGC	3 cut(s)	54, 648, 1732
BstDEI	CTNAG	5 cut(s)	411, 992, 1103, 1238, 1290
BstDSI	CCRYGG	1 cut(s)	703
BstF5I	GGATG	9 cut(s)	261, 388, 837, 1150, 1166, 1186, 1273, 1721, 1924
BstFNI	CGCG	1 cut(s)	475
BstHHI	GCGC	1 cut(s)	475
BstKTI	GATC	6 cut(s)	600, 611, 985, 1467, 1487, 2170
BstMAI	GTCTC	2 cut(s)	2112, 2149
BstMBI	GATC	6 cut(s)	597, 608, 982, 1464, 1484, 2167
BstMCI	CGRYCG	1 cut(s)	600
BstMWI	GCNNNNNNNGC	8 cut(s)	281, 564, 573, 953, 1147, 1321, 1438, 1829
BstNI	CCWGG	4 cut(s)	75, 533, 578, 1397
BstNSI	RCATGY	2 cut(s)	56, 650
BstSCI	CCNGG	6 cut(s)	73, 305, 531, 576, 793, 1395
BstSFI	CTRYAG	3 cut(s)	285, 1142, 2204
BstUI	CGCG	1 cut(s)	475
BstV1I	GCAGC	6 cut(s)	212, 394, 675, 940, 1128, 1819
BstV2I	GAAGAC	6 cut(s)	74, 338, 470, 1274, 1626, 1756
BstXI	CCANNNNNNTGG	2 cut(s)	1177, 1519
BsuI	GTATCC	2 cut(s)	417, 1942
BsuRI	GGCC	5 cut(s)	708, 1315, 1400, 1707, 2070
BtgI	CCRYGG	1 cut(s)	703
BtsCI	GGATG	9 cut(s)	261, 388, 837, 1150, 1166, 1186, 1273, 1721, 1924
BtsI	GCAGTG	1 cut(s)	1151
BtsIMutI	CAGTG	7 cut(s)	214, 259, 696, 855, 1151, 1165, 2152
Cac8I	GCNNGC	3 cut(s)	54, 648, 1732
CaiI	CAGNNNCTG	1 cut(s)	1304
CfoI	GCGC	1 cut(s)	475
Cfr13I	GGNCC	5 cut(s)	257, 386, 1313, 2068, 2099
CseI	GACGC	2 cut(s)	79, 464
Csp6I	GTAC	5 cut(s)	1093, 1202, 1336, 1683, 1801
CviQI	GTAC	5 cut(s)	1093, 1202, 1336, 1683, 1801
DdeI	CTNAG	5 cut(s)	411, 992, 1103, 1238, 1290
DpnI	GATC	6 cut(s)	599, 610, 984, 1466, 1486, 2169
DpnII	GATC	6 cut(s)	597, 608, 982, 1464, 1484, 2167
DraI	TTTAAA	1 cut(s)	511
DraIII	CACNNNGTG	2 cut(s)	1797, 2147
DriI	GACNNNNNGTC	1 cut(s)	1522
EaeI	YGGCCR	1 cut(s)	1398
Eam1104I	CTCTTC	7 cut(s)	306, 437, 698, 1006, 1506, 1599, 2115
Eam1105I	GACNNNNNGTC	1 cut(s)	1522
EarI	CTCTTC	7 cut(s)	306, 437, 698, 1006, 1506, 1599, 2115
Ecl136II	GAGCTC	1 cut(s)	319
Eco24I	GRGCYC	2 cut(s)	321, 1812
Eco47I	GGWCC	3 cut(s)	257, 386, 2099
Eco53kI	GAGCTC	1 cut(s)	319
Eco57I	CTGAAG	3 cut(s)	125, 734, 1544
Eco88I	CYCGRG	1 cut(s)	1581
EcoICRI	GAGCTC	1 cut(s)	319
EcoO109I	RGGNCCY	1 cut(s)	1313
EcoRII	CCWGG	4 cut(s)	73, 531, 576, 1395
EcoT38I	GRGCYC	2 cut(s)	321, 1812
Esp3I	CGTCTC	1 cut(s)	2112
FalI	AAGNNNNNCTT	2 cut(s)	1797, 1829
FaqI	GGGAC	4 cut(s)	243, 558, 1629, 1898
FblI	GTMKAC	1 cut(s)	1447
Fnu4HI	GCNGC	6 cut(s)	201, 408, 689, 954, 1142, 1833
FokI	GGATG	9 cut(s)	248, 395, 844, 1137, 1153, 1193, 1280, 1708, 1931
FriOI	GRGCYC	2 cut(s)	321, 1812
Fsp4HI	GCNGC	6 cut(s)	201, 408, 689, 954, 1142, 1833
FspBI	CTAG	4 cut(s)	603, 671, 719, 1008
GlaI	GCGC	1 cut(s)	474
GluI	GCNGC	6 cut(s)	201, 408, 689, 954, 1142, 1833
GsuI	CTGGAG	1 cut(s)	831
HaeIII	GGCC	5 cut(s)	708, 1315, 1400, 1707, 2070
HapII	CCGG	2 cut(s)	306, 795
HgaI	GACGC	2 cut(s)	79, 464
HhaI	GCGC	1 cut(s)	475
Hin1I	GRCGYC	1 cut(s)	71
Hin6I	GCGC	1 cut(s)	473
HinP1I	GCGC	1 cut(s)	473
HincII	GTYRAC	3 cut(s)	1296, 1527, 1936
HindII	GTYRAC	3 cut(s)	1296, 1527, 1936
HinfI	GANTC	6 cut(s)	100, 996, 1523, 1564, 1586, 1631
HpaI	GTTAAC	2 cut(s)	1296, 1936
HpaII	CCGG	2 cut(s)	306, 795
HphI	GGTGA	2 cut(s)	509, 944
Hpy166II	GTNNAC	8 cut(s)	85, 703, 1021, 1296, 1448, 1527, 1573, 1936
Hpy188I	TCNGA	5 cut(s)	66, 105, 1489, 1563, 1591
Hpy188III	TCNNGA	7 cut(s)	231, 980, 1008, 1127, 1178, 1405, 1583
Hpy8I	GTNNAC	8 cut(s)	85, 703, 1021, 1296, 1448, 1527, 1573, 1936
Hpy99I	CGWCG	1 cut(s)	1631
HpyAV	CCTTC	6 cut(s)	38, 758, 818, 1052, 1258, 1304
HpyCH4III	ACNGT	8 cut(s)	217, 700, 822, 1057, 1335, 1627, 2053, 2147
HpyCH4IV	ACGT	2 cut(s)	927, 1575
HpyCH4V	TGCA	8 cut(s)	113, 200, 925, 947, 1144, 1278, 1994, 2021
HpyF10VI	GCNNNNNNNGC	8 cut(s)	281, 564, 573, 953, 1147, 1321, 1438, 1829
HpyF3I	CTNAG	5 cut(s)	411, 992, 1103, 1238, 1290
HpySE526I	ACGT	2 cut(s)	927, 1575
Hsp92I	GRCGYC	1 cut(s)	71
HspAI	GCGC	1 cut(s)	473
KspAI	GTTAAC	2 cut(s)	1296, 1936
Kzo9I	GATC	6 cut(s)	597, 608, 982, 1464, 1484, 2167
LguI	GCTCTTC	1 cut(s)	698
LmnI	GCTCC	5 cut(s)	316, 324, 850, 1815, 1906
Lsp1109I	GCAGC	6 cut(s)	212, 394, 675, 940, 1128, 1819
LweI	GCATC	2 cut(s)	1159, 1885
MaeI	CTAG	4 cut(s)	603, 671, 719, 1008
MaeII	ACGT	2 cut(s)	927, 1575
MaeIII	GTNAC	4 cut(s)	497, 932, 1576, 2139
MalI	GATC	6 cut(s)	599, 610, 984, 1466, 1486, 2169
MboI	GATC	6 cut(s)	597, 608, 982, 1464, 1484, 2167
MfeI	CAATTG	1 cut(s)	561
MhlI	GDGCHC	2 cut(s)	321, 1812
MlsI	TGGCCA	1 cut(s)	1400
MluNI	TGGCCA	1 cut(s)	1400
MlyI	GAGTC	3 cut(s)	1532, 1558, 1640
Mox20I	TGGCCA	1 cut(s)	1400
MroXI	GAANNNNTTC	1 cut(s)	1065
MscI	TGGCCA	1 cut(s)	1400
MseI	TTAA	5 cut(s)	510, 1253, 1295, 1479, 1935
MslI	CAYNNNNRTG	4 cut(s)	905, 930, 1517, 2016
Msp20I	TGGCCA	1 cut(s)	1400
MspI	CCGG	2 cut(s)	306, 795
MspR9I	CCNGG	6 cut(s)	75, 307, 533, 578, 795, 1397
MunI	CAATTG	1 cut(s)	561
Mva1269I	GAATGC	1 cut(s)	2007
MvaI	CCWGG	4 cut(s)	75, 533, 578, 1397
MvnI	CGCG	1 cut(s)	475
MwoI	GCNNNNNNNGC	8 cut(s)	281, 564, 573, 953, 1147, 1321, 1438, 1829
NciI	CCSGG	2 cut(s)	307, 795
NdeII	GATC	6 cut(s)	597, 608, 982, 1464, 1484, 2167
NlaIV	GGNNCC	4 cut(s)	259, 1054, 1811, 2069
NmeAIII	GCCGAG	1 cut(s)	65
NmuCI	GTSAC	4 cut(s)	497, 932, 1576, 2139
NspI	RCATGY	2 cut(s)	56, 650
OliI	CACNNNNGTG	1 cut(s)	930
PaeI	GCATGC	2 cut(s)	56, 650
PciSI	GCTCTTC	1 cut(s)	698
PctI	GAATGC	1 cut(s)	2007
PdmI	GAANNNNTTC	1 cut(s)	1065
PfeI	GAWTC	3 cut(s)	100, 996, 1586
PflMI	CCANNNNNTGG	1 cut(s)	1797
PfoI	TCCNGGA	2 cut(s)	531, 793
PkrI	GCNGC	6 cut(s)	202, 409, 690, 955, 1143, 1834
Ple19I	CGATCG	1 cut(s)	600
PleI	GAGTC	3 cut(s)	1531, 1558, 1639
PpsI	GAGTC	3 cut(s)	1531, 1558, 1639
PsiI	TTATAA	1 cut(s)	1208
Psp124BI	GAGCTC	1 cut(s)	321
Psp6I	CCWGG	4 cut(s)	73, 531, 576, 1395
PspGI	CCWGG	4 cut(s)	73, 531, 576, 1395
PspN4I	GGNNCC	4 cut(s)	259, 1054, 1811, 2069
PspPI	GGNCC	5 cut(s)	257, 386, 1313, 2068, 2099
PsrI	GAACNNNNNNTAC	2 cut(s)	1050, 1082
PstI	CTGCAG	1 cut(s)	1146
PstNI	CAGNNNCTG	1 cut(s)	1304
PvuI	CGATCG	1 cut(s)	600
RsaI	GTAC	5 cut(s)	1094, 1203, 1337, 1684, 1802
RsaNI	GTAC	5 cut(s)	1093, 1202, 1336, 1683, 1801
RseI	CAYNNNNRTG	4 cut(s)	905, 930, 1517, 2016
SacI	GAGCTC	1 cut(s)	321
SapI	GCTCTTC	1 cut(s)	698
SaqAI	TTAA	5 cut(s)	510, 1253, 1295, 1479, 1935
SatI	GCNGC	6 cut(s)	201, 408, 689, 954, 1142, 1833
Sau3AI	GATC	6 cut(s)	597, 608, 982, 1464, 1484, 2167
Sau96I	GGNCC	5 cut(s)	257, 386, 1313, 2068, 2099
SchI	GAGTC	3 cut(s)	1532, 1558, 1640
ScrFI	CCNGG	6 cut(s)	75, 307, 533, 578, 795, 1397
SduI	GDGCHC	2 cut(s)	321, 1812
SfaNI	GCATC	2 cut(s)	1159, 1885
SfcI	CTRYAG	3 cut(s)	285, 1142, 2204
SinI	GGWCC	3 cut(s)	257, 386, 2099
SmiMI	CAYNNNNRTG	4 cut(s)	905, 930, 1517, 2016
SmlI	CTYRAG	1 cut(s)	1405
SmoI	CTYRAG	1 cut(s)	1405
SphI	GCATGC	2 cut(s)	56, 650
SsiI	CCGC	2 cut(s)	1090, 1475
SspI	AATATT	1 cut(s)	759
SspMI	CTAG	4 cut(s)	603, 671, 719, 1008
SstI	GAGCTC	1 cut(s)	321
StyD4I	CCNGG	6 cut(s)	73, 305, 531, 576, 793, 1395
TaaI	ACNGT	8 cut(s)	217, 700, 822, 1057, 1335, 1627, 2053, 2147
TaiI	ACGT	2 cut(s)	930, 1578
TaqI	TCGA	3 cut(s)	35, 1262, 1629
TatI	WGTACW	1 cut(s)	1201
TfiI	GAWTC	3 cut(s)	100, 996, 1586
Tru1I	TTAA	5 cut(s)	510, 1253, 1295, 1479, 1935
Tru9I	TTAA	5 cut(s)	510, 1253, 1295, 1479, 1935
TscAI	CASTG	7 cut(s)	214, 259, 703, 855, 1151, 1165, 2152
TseFI	GTSAC	4 cut(s)	497, 932, 1576, 2139
TseI	GCWGC	6 cut(s)	200, 407, 688, 953, 1141, 1832
Tsp45I	GTSAC	4 cut(s)	497, 932, 1576, 2139
TspDTI	ATGAA	8 cut(s)	123, 424, 530, 1365, 1772, 1807, 2000, 2052
TspGWI	ACGGA	1 cut(s)	2094
TspRI	CASTG	7 cut(s)	214, 259, 703, 855, 1151, 1165, 2152
Van91I	CCANNNNNTGG	1 cut(s)	1797
VpaK11BI	GGWCC	3 cut(s)	257, 386, 2099
XapI	RAATTY	5 cut(s)	167, 537, 862, 1614, 2040
XbaI	TCTAGA	1 cut(s)	1007
XceI	RCATGY	2 cut(s)	56, 650
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2078
XmiI	GTMKAC	1 cut(s)	1447
XmnI	GAANNNNTTC	1 cut(s)	1065
XspI	CTAG	4 cut(s)	603, 671, 719, 1008

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.