Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003400

Physical Location & Seq

Genomic Coordinates Forward (+)

10377869 .. 10381891

Gene Structure 4023 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1140500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGGATGATGAAATTGGTGTCCATAACCCCCTTCTTTCGAGCCATCCTTCTCGACGTGCCTTCGTCCGGAAGATACCTCTTTTCGTAACCTTGGCGAAATGGATTCTCAAGATCACAATGTGGGTGCTTTTCATTTCATGGGTGGTTTTCATTTTTCTGTTCCCTCTAGGCTCTGTGCAGACATTTTTTGGTGACTGGATTGCAGCCACCGATGGAACTCTTTTCGGGACTACAGGAACCCTTCTCCTTCTGTTCAACGGTCCTCTTCTCGTTATTGCCTTGCTTTCGATAGCATTTCTAGCTATGTCTGGGGAAGAAGAGCTCCCCCACAAGAAGAAGAAGACTTCACAAAACCCAAGTTTCCGCTTGTGGACATTCCCAATTCTTGTGGATGGACCATTTGGGGTTGTTTCTGCTGCTGAGTTGATTGGGATATTACTCTTTGTTGTGTATACTATGTGGTCTGTGTCCTATTATGCCGTGAGCATTCTCGGCACGATACCTGGTTCGTTAAGTCTTAAAGCACAAAGTTGTTTGTTCCTGGCAATTTTCGGGGCTTACTTAGCCTCGAGTGGGATGTTTTGCTTGGCATTTTTGTTCCTTCCTATTGCAAGGGGATCAGTTCTTCTTCGCCTTATAGATATCCCTTTCGAGCATGCTACGAGATATCATGTATGGCTGGGACATCTCACGATGTCGCTCTTTACTCTCCATGGACTATGTTACATAGTGACATGGGCAATGGAGGGCAATCTCCTTGCTAAAATATTGGAGTGGAGGGAGATTGGTGTTGCAAATCTTGCAGGAGTAATCAGCCTTGCTGCTGGTCTGTTGATGTGGGTCACATCGCTCCCTCCAGTTAGAAAGCTGAATTTTGAGTTGTTCTTCTACACTCATCAACTATATGTCATCTTCATTGCCTTCTTGGCTATGCACGTTGGTGATTTTCTTTTCGTCTGTGCTGCCGGAGGCATATTTATTTATATCCTTGATCGTTTTCTGAGATTCTGCCAATCGCGAAGGACTGTTGGTGTAATCTCAGCCAAGTGCCTTCCATGCGGAACTGTAGAATTAGTCCTTGCAAAACCTGAAAATCTGCAGTACAACGCGCTCAGTTTTATTTTTCTTCAAGTCCGGGAACTGTCATGGCTTCAATGGCATCCTTTTAGCGTTTCATCCAGTCCTCTGGACGGTAAGTATCACATTTCCGTCCTCATCAAGGTTCTTGGGAAATGGACAGAAAATCTTCGAGGAAAGATCTTGAAAAATTCGGAGGCTGAACAACAACAAAGCGCGCTCTCCTGCACGCCTCCTACGACGCTAACAGTGTCTGTTGAGGGGCCTTATGGGCATGAGATACCATACCACTTGGTGTATGAACATCTGGTCTTAGTAGCGGGCGGCATTGGGATTTCGCCTTTCCTGGCTGTCCTGAGTGACATTGTCCATTGTGTTAAGGAAGGGAGATTGTGTCAAGTACGAAATATTTTGATTGTCTGGGCTGTCAAAACATCAAATGAGCTTCCTCTTCTTTCAACCATCGACATGGACTCAGTTTCCAGTAAACTAAATATTCAGACTCTAATTTACGTCACTCGGGAATCTGGTCCTCCGTTGGAAGAGGGTCAAGTTCTGAACAGCGCGCTGGAGTTTCCTCCTCGTTGTCCTCCTAGGCCGAAAAGGTGTAGCATGTCTGTTTTGGTTGGTTCCGGAGATAATCTATGGTCCGGACTATATCTCATCTCATCGACGGTAGGCTTAGTTATCATATTGGGTTTGTTGGATGTCTTGTACATAAACCCGTACCGTATATCTGCATGGTGGTACAAAGGGCTTCTGTTGCTGCTCTGTATGGTTGCAAGTGTGGTCGTCTTTGGGGGTCTTGTGGTTGGTTTATGGCATCTTTGGGAGCGAAAATTTTCGCCGCAGGATCAATGCGAAGACGATACTTCAGATGTATATAAGGCGGAGCATGAAGAAACTATAGGTCGCAGTGATTCAAATGAGCACCGAGAAAATCTTTCAAAGTTAACAGGCATTCGTTATGGTGCAAGACCGAACTTCAAAGAAATTTTTGGAACCATATCCAAGGATTGGGGTACCGTCGACGCGGGCGTCATTGTTTGTGGTCCTCCAAGTTTTGAAGCAAGTGTTGCCAAAGAGATCAGGTCACACAATTTGAGGAGACAACGTGACGATCCTATCTTCCATTTCAACAGTCACAGTTTCGATCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000293 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006091 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009767 GO:0009987 GO:0015979 GO:0016020 GO:0016491 GO:0016722 GO:0016723 GO:0019684 GO:0022900 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0055114 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NAD_binding_6

Protein Analysis

746

Amino Acids

83.18

Weight (kDa)

7.29

Isoelectric Point (pI)

37.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ferric_reduct	PF01794	192 - 309	2.7e-21	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	345 - 451	1.6e-19	FAD-binding domain
NAD_binding_6	PF08030	459 - 535	4.4e-09	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49730 AT5G49730 AT5G49740 AT5G49740 AT5G49740
fragaria_vesca	FvH4_5g27150 FvH4_5g27150 FvH4_5g27150 FvH4_5g27150 FvH4_5g27150
malus_domestica	MD08G1250800.v1.1 MD15G1140500.v1.1
prunus_persica	Prupe.1G587800_v2.0.a1 Prupe.1G587800_v2.0.a1
pyrus_communis	pycom08g21770 pycom15g12700
rosa_chinensis	RchiOBHm_Chr7g0218161 RchiOBHm_Chr7g0218171
rosa_laevigata	RLG00000002392
rosa_multiflora	Rmu_sc0003627.1_g000006 Rmu_sc0003627.1_g000007
rosa_roxburghii	Rroxscaffold_3G00241250 Rroxscaffold_3G00241260
rosa_rugosa	Rorug07G0174700 Rorug07G0174700 Rorug07G0174800
rosa_samantha	Rh7AG317100 Rh7AG317200 Rh7BG308300 Rh7BG315800 Rh7CG333900 Rh7CG334000 Rh7DG316000 Rh7DG316200
rosa_wichuraiana	Rw7G026830 Rw7G026840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2114
Acc65I	GGTACC	1 cut(s)	2099
AccB1I	GGYRCC	1 cut(s)	2099
AccB7I	CCANNNNNTGG	1 cut(s)	2094
AccI	GTMKAC	2 cut(s)	452, 2106
AccII	CGCG	5 cut(s)	1018, 1109, 1295, 1643, 2111
AccIII	TCCGGA	3 cut(s)	66, 1709, 1727
AciI	CCGC	7 cut(s)	364, 1059, 1397, 1401, 1925, 1967, 2111
AclWI	GGATC	3 cut(s)	625, 1938, 2192
AcsI	RAATTY	4 cut(s)	871, 1267, 1916, 2070
AcuI	CTGAAG	1 cut(s)	1935
AcyI	GRCGYC	1 cut(s)	2115
AdeI	CACNNNGTG	2 cut(s)	120, 1372
AfaI	GTAC	6 cut(s)	1103, 1479, 1793, 1805, 1826, 2101
AfiI	CCNNNNNNNGG	5 cut(s)	66, 573, 1190, 1219, 2094
AjiI	CACGTC	1 cut(s)	56
AjnI	CCWGG	3 cut(s)	502, 540, 1422
AleI	CACNNNNGTG	1 cut(s)	939
AluBI	AGCT	4 cut(s)	302, 322, 868, 1522
AluI	AGCT	4 cut(s)	302, 322, 868, 1522
Alw21I	GWGCWC	2 cut(s)	324, 2010
Alw26I	GTCTC	1 cut(s)	2179
AlwI	GGATC	3 cut(s)	625, 1938, 2192
AlwNI	CAGNNNCTG	1 cut(s)	1331
Ama87I	CYCGRG	2 cut(s)	568, 1596
Aor13HI	TCCGGA	3 cut(s)	66, 1709, 1727
AoxI	GGCC	2 cut(s)	1340, 1673
ApeKI	GCWGC	5 cut(s)	203, 416, 821, 962, 1843
ApoI	RAATTY	4 cut(s)	871, 1267, 1916, 2070
Asp700I	GAANNNNTTC	2 cut(s)	1245, 1918
Asp718I	GGTACC	1 cut(s)	2099
AspA2I	CCTAGG	1 cut(s)	1670
AspLEI	GCGC	5 cut(s)	1111, 1295, 1297, 1643, 1645
AspS9I	GGNCC	6 cut(s)	260, 395, 1340, 1607, 1725, 2129
AsuC2I	CCSGG	1 cut(s)	1136
AsuHPI	GGTGA	2 cut(s)	203, 953
AvaI	CYCGRG	2 cut(s)	568, 1596
AvaII	GGWCC	5 cut(s)	260, 395, 1607, 1725, 2129
AvrII	CCTAGG	1 cut(s)	1670
BanI	GGYRCC	1 cut(s)	2099
BanII	GRGCYC	1 cut(s)	324
BbsI	GAAGAC	2 cut(s)	347, 1947
Bbv12I	GWGCWC	2 cut(s)	324, 2010
BbvI	GCAGC	5 cut(s)	215, 403, 808, 949, 1830
BccI	CCATC	4 cut(s)	51, 206, 386, 1547
BceAI	ACGGC	1 cut(s)	464
BciT130I	CCWGG	3 cut(s)	504, 542, 1424
BcnI	CCSGG	1 cut(s)	1136
BcoDI	GTCTC	1 cut(s)	2179
BfaI	CTAG	3 cut(s)	167, 299, 1671
BfmI	CTRYAG	5 cut(s)	231, 1065, 1097, 1983, 2234
BglI	GCCNNNNNGGC	2 cut(s)	926, 1348
BglII	AGATCT	1 cut(s)	1257
BisI	GCNGC	7 cut(s)	204, 417, 822, 963, 1402, 1844, 1925
BlnI	CCTAGG	1 cut(s)	1670
BlsI	GCNGC	7 cut(s)	205, 418, 823, 964, 1403, 1845, 1926
Bme1390I	CCNGG	4 cut(s)	504, 542, 1136, 1424
Bme18I	GGWCC	5 cut(s)	260, 395, 1607, 1725, 2129
BmeT110I	CYCGRG	2 cut(s)	568, 1596
BmgBI	CACGTC	1 cut(s)	56
BmgT120I	GGNCC	6 cut(s)	260, 395, 1340, 1607, 1725, 2129
BmiI	GGNNCC	5 cut(s)	238, 1341, 1708, 2080, 2101
BmrFI	CCNGG	4 cut(s)	504, 542, 1136, 1424
BmsI	GCATC	2 cut(s)	1168, 1909
BpiI	GAAGAC	2 cut(s)	347, 1947
BpmI	CTGGAG	2 cut(s)	840, 1667
BpuEI	CTTGAG	1 cut(s)	92
BpuMI	CCSGG	1 cut(s)	1136
BsaHI	GRCGYC	1 cut(s)	2115
BsaJI	CCNNGG	4 cut(s)	90, 712, 1670, 2088
BsaWI	WCCGGW	3 cut(s)	66, 1709, 1727
BsaXI	ACNNNNNCTCC	4 cut(s)	1456, 1486, 2176, 2206
Bsc4I	CCNNNNNNNGG	5 cut(s)	66, 573, 1190, 1219, 2094
Bse1I	ACTGG	4 cut(s)	200, 857, 1179, 1560
Bse3DI	GCAATG	2 cut(s)	747, 915
BseAI	TCCGGA	3 cut(s)	66, 1709, 1727
BseBI	CCWGG	3 cut(s)	504, 542, 1424
BseDI	CCNNGG	4 cut(s)	90, 712, 1670, 2088
BseGI	GGATG	7 cut(s)	10, 43, 397, 582, 1159, 1175, 1789
BseLI	CCNNNNNNNGG	5 cut(s)	66, 573, 1190, 1219, 2094
BseMI	GCAATG	2 cut(s)	747, 915
BseMII	CTCAG	6 cut(s)	411, 992, 1053, 1126, 1424, 1566
BseNI	ACTGG	4 cut(s)	200, 857, 1179, 1560
BsePI	GCGCGC	2 cut(s)	1293, 1641
BseRI	GAGGAG	2 cut(s)	1647, 2197
BseXI	GCAGC	5 cut(s)	215, 403, 808, 949, 1830
BseYI	CCCAGC	1 cut(s)	679
BsgI	GTGCAG	2 cut(s)	197, 1288
Bsh1236I	CGCG	5 cut(s)	1018, 1109, 1295, 1643, 2111
BshFI	GGCC	2 cut(s)	1342, 1675
BshNI	GGYRCC	1 cut(s)	2099
BsiHKAI	GWGCWC	2 cut(s)	324, 2010
BsiHKCI	CYCGRG	2 cut(s)	568, 1596
BsiSI	CCGG	5 cut(s)	67, 966, 1135, 1710, 1728
BslFI	GGGAC	2 cut(s)	241, 696
BslI	CCNNNNNNNGG	5 cut(s)	66, 573, 1190, 1219, 2094
BsmAI	GTCTC	1 cut(s)	2179
BsmFI	GGGAC	2 cut(s)	241, 696
BsmI	GAATGC	2 cut(s)	486, 2037
BsnI	GGCC	2 cut(s)	1342, 1675
BsoBI	CYCGRG	2 cut(s)	568, 1596
Bsp1286I	GDGCHC	2 cut(s)	324, 2010
Bsp13I	TCCGGA	3 cut(s)	66, 1709, 1727
Bsp1407I	TGTACA	1 cut(s)	1791
Bsp143I	GATC	8 cut(s)	111, 617, 991, 1257, 1930, 2163, 2197, 2230
Bsp19I	CCATGG	1 cut(s)	712
Bsp68I	TCGCGA	1 cut(s)	1018
BspACI	CCGC	7 cut(s)	364, 1059, 1397, 1401, 1925, 1967, 2111
BspANI	GGCC	2 cut(s)	1342, 1675
BspCNI	CTCAG	6 cut(s)	412, 993, 1052, 1125, 1425, 1565
BspEI	TCCGGA	3 cut(s)	66, 1709, 1727
BspFNI	CGCG	5 cut(s)	1018, 1109, 1295, 1643, 2111
BspLI	GGNNCC	5 cut(s)	238, 1341, 1708, 2080, 2101
BspMAI	CTGCAG	1 cut(s)	1101
BspPI	GGATC	3 cut(s)	625, 1938, 2192
BspQI	GCTCTTC	1 cut(s)	312
BspT107I	GGYRCC	1 cut(s)	2099
BsrDI	GCAATG	2 cut(s)	747, 915
BsrGI	TGTACA	1 cut(s)	1791
BsrI	ACTGG	4 cut(s)	200, 857, 1179, 1560
BssECI	CCNNGG	4 cut(s)	90, 712, 1670, 2088
BssHII	GCGCGC	2 cut(s)	1293, 1641
BssMI	GATC	8 cut(s)	111, 617, 991, 1257, 1930, 2163, 2197, 2230
BssNAI	GTATAC	1 cut(s)	453
BssNI	GRCGYC	1 cut(s)	2115
BssT1I	CCWWGG	4 cut(s)	90, 712, 1670, 2088
Bst1107I	GTATAC	1 cut(s)	453
Bst2UI	CCWGG	3 cut(s)	504, 542, 1424
Bst6I	CTCTTC	4 cut(s)	270, 312, 1533, 1614
BstACI	GRCGYC	1 cut(s)	2115
BstAPI	GCANNNNNTGC	2 cut(s)	800, 2153
BstAUI	TGTACA	1 cut(s)	1791
BstC8I	GCNNGC	6 cut(s)	657, 1295, 1307, 1399, 1643, 2113
BstDEI	CTNAG	9 cut(s)	420, 562, 1001, 1039, 1112, 1390, 1433, 1552, 1759
BstDSI	CCRYGG	1 cut(s)	712
BstENI	CCTNNNNNAGG	1 cut(s)	1217
BstF5I	GGATG	7 cut(s)	10, 43, 397, 582, 1159, 1175, 1789
BstFNI	CGCG	5 cut(s)	1018, 1109, 1295, 1643, 2111
BstHHI	GCGC	5 cut(s)	1111, 1295, 1297, 1643, 1645
BstKTI	GATC	8 cut(s)	114, 620, 994, 1260, 1933, 2166, 2200, 2233
BstMAI	GTCTC	1 cut(s)	2179
BstMBI	GATC	8 cut(s)	111, 617, 991, 1257, 1930, 2163, 2197, 2230
BstNI	CCWGG	3 cut(s)	504, 542, 1424
BstNSI	RCATGY	2 cut(s)	659, 1693
BstSCI	CCNGG	4 cut(s)	502, 540, 1134, 1422
BstSFI	CTRYAG	5 cut(s)	231, 1065, 1097, 1983, 2234
BstUI	CGCG	5 cut(s)	1018, 1109, 1295, 1643, 2111
BstV1I	GCAGC	5 cut(s)	215, 403, 808, 949, 1830
BstV2I	GAAGAC	2 cut(s)	347, 1947
BstX2I	RGATCY	1 cut(s)	1257
BstXI	CCANNNNNNTGG	2 cut(s)	1186, 1546
BstYI	RGATCY	1 cut(s)	1257
BstZ17I	GTATAC	1 cut(s)	453
BsuRI	GGCC	2 cut(s)	1342, 1675
BtgI	CCRYGG	1 cut(s)	712
BtgZI	GCGATG	1 cut(s)	831
BtrI	CACGTC	1 cut(s)	56
BtsCI	GGATG	7 cut(s)	10, 43, 397, 582, 1159, 1175, 1789
BtsI	GCAGTG	1 cut(s)	1999
BtsIMutI	CAGTG	2 cut(s)	1332, 1999
BtuMI	TCGCGA	1 cut(s)	1018
Cac8I	GCNNGC	6 cut(s)	657, 1295, 1307, 1399, 1643, 2113
CaiI	CAGNNNCTG	1 cut(s)	1331
CfoI	GCGC	5 cut(s)	1111, 1295, 1297, 1643, 1645
Cfr13I	GGNCC	6 cut(s)	260, 395, 1340, 1607, 1725, 2129
CseI	GACGC	3 cut(s)	1327, 2104, 2117
CsiI	ACCWGGT	1 cut(s)	502
Csp6I	GTAC	6 cut(s)	1102, 1478, 1792, 1804, 1825, 2100
CspCI	CAANNNNNGTGG	2 cut(s)	369, 404
CviQI	GTAC	6 cut(s)	1102, 1478, 1792, 1804, 1825, 2100
DdeI	CTNAG	9 cut(s)	420, 562, 1001, 1039, 1112, 1390, 1433, 1552, 1759
DpnI	GATC	8 cut(s)	113, 619, 993, 1259, 1932, 2165, 2199, 2232
DpnII	GATC	8 cut(s)	111, 617, 991, 1257, 1930, 2163, 2197, 2230
DraIII	CACNNNGTG	2 cut(s)	120, 1372
DrdI	GACNNNNNNGTC	1 cut(s)	2114
DseDI	GACNNNNNNGTC	1 cut(s)	2114
Eam1104I	CTCTTC	4 cut(s)	270, 312, 1533, 1614
EarI	CTCTTC	4 cut(s)	270, 312, 1533, 1614
EciI	GGCGGA	1 cut(s)	1982
Ecl136II	GAGCTC	1 cut(s)	322
Eco130I	CCWWGG	4 cut(s)	90, 712, 1670, 2088
Eco24I	GRGCYC	1 cut(s)	324
Eco32I	GATATC	2 cut(s)	643, 668
Eco47I	GGWCC	5 cut(s)	260, 395, 1607, 1725, 2129
Eco53kI	GAGCTC	1 cut(s)	322
Eco57I	CTGAAG	1 cut(s)	1935
Eco88I	CYCGRG	2 cut(s)	568, 1596
EcoICRI	GAGCTC	1 cut(s)	322
EcoNI	CCTNNNNNAGG	1 cut(s)	1217
EcoO109I	RGGNCCY	1 cut(s)	1340
EcoRII	CCWGG	3 cut(s)	502, 540, 1422
EcoRV	GATATC	2 cut(s)	643, 668
EcoT14I	CCWWGG	4 cut(s)	90, 712, 1670, 2088
EcoT38I	GRGCYC	1 cut(s)	324
ErhI	CCWWGG	4 cut(s)	90, 712, 1670, 2088
FaqI	GGGAC	2 cut(s)	241, 696
FauI	CCCGC	2 cut(s)	1390, 2104
FblI	GTMKAC	2 cut(s)	452, 2106
Fnu4HI	GCNGC	7 cut(s)	204, 417, 822, 963, 1402, 1844, 1925
FokI	GGATG	7 cut(s)	17, 30, 404, 589, 1146, 1162, 1796
FriOI	GRGCYC	1 cut(s)	324
Fsp4HI	GCNGC	7 cut(s)	204, 417, 822, 963, 1402, 1844, 1925
FspBI	CTAG	3 cut(s)	167, 299, 1671
GlaI	GCGC	5 cut(s)	1110, 1294, 1296, 1642, 1644
GluI	GCNGC	7 cut(s)	204, 417, 822, 963, 1402, 1844, 1925
GsaI	CCCAGC	1 cut(s)	683
GsuI	CTGGAG	2 cut(s)	840, 1667
HaeIII	GGCC	2 cut(s)	1342, 1675
HapII	CCGG	5 cut(s)	67, 966, 1135, 1710, 1728
HgaI	GACGC	3 cut(s)	1327, 2104, 2117
HhaI	GCGC	5 cut(s)	1111, 1295, 1297, 1643, 1645
Hin1I	GRCGYC	1 cut(s)	2115
Hin6I	GCGC	5 cut(s)	1109, 1293, 1295, 1641, 1643
HinP1I	GCGC	5 cut(s)	1109, 1293, 1295, 1641, 1643
HincII	GTYRAC	2 cut(s)	2031, 2107
HindII	GTYRAC	2 cut(s)	2031, 2107
HinfI	GANTC	6 cut(s)	103, 1005, 1550, 1579, 1601, 1997
HpaI	GTTAAC	1 cut(s)	2031
HpaII	CCGG	5 cut(s)	67, 966, 1135, 1710, 1728
HphI	GGTGA	2 cut(s)	203, 953
Hpy166II	GTNNAC	5 cut(s)	372, 453, 1565, 2031, 2107
Hpy188I	TCNGA	5 cut(s)	1002, 1273, 1578, 1635, 1954
Hpy8I	GTNNAC	5 cut(s)	372, 453, 1565, 2031, 2107
Hpy99I	CGWCG	5 cut(s)	57, 1321, 1753, 2108, 2111
HpyCH4IV	ACGT	4 cut(s)	55, 936, 1590, 2191
HpyF3I	CTNAG	9 cut(s)	420, 562, 1001, 1039, 1112, 1390, 1433, 1552, 1759
HpySE526I	ACGT	4 cut(s)	55, 936, 1590, 2191
Hsp92I	GRCGYC	1 cut(s)	2115
HspAI	GCGC	5 cut(s)	1109, 1293, 1295, 1641, 1643
Kpn2I	TCCGGA	3 cut(s)	66, 1709, 1727
KpnI	GGTACC	1 cut(s)	2103
KspAI	GTTAAC	1 cut(s)	2031
Kzo9I	GATC	8 cut(s)	111, 617, 991, 1257, 1930, 2163, 2197, 2230
LguI	GCTCTTC	1 cut(s)	312
LmnI	GCTCC	4 cut(s)	327, 855, 1909, 1969
Lsp1109I	GCAGC	5 cut(s)	215, 403, 808, 949, 1830
LweI	GCATC	2 cut(s)	1168, 1909
MabI	ACCWGGT	1 cut(s)	502
MaeI	CTAG	3 cut(s)	167, 299, 1671
MaeII	ACGT	4 cut(s)	55, 936, 1590, 2191
MalI	GATC	8 cut(s)	113, 619, 993, 1259, 1932, 2165, 2199, 2232
MboI	GATC	8 cut(s)	111, 617, 991, 1257, 1930, 2163, 2197, 2230
MflI	RGATCY	1 cut(s)	1257
MhlI	GDGCHC	2 cut(s)	324, 2010
MlyI	GAGTC	2 cut(s)	1544, 1573
MmeI	TCCRAC	2 cut(s)	1596, 1761
MroI	TCCGGA	3 cut(s)	66, 1709, 1727
MroXI	GAANNNNTTC	2 cut(s)	1245, 1918
MseI	TTAA	4 cut(s)	512, 519, 1455, 2030
MslI	CAYNNNNRTG	2 cut(s)	939, 1544
MspI	CCGG	5 cut(s)	67, 966, 1135, 1710, 1728
MspR9I	CCNGG	4 cut(s)	504, 542, 1136, 1424
Mva1269I	GAATGC	2 cut(s)	486, 2037
MvaI	CCWGG	3 cut(s)	504, 542, 1424
MvnI	CGCG	5 cut(s)	1018, 1109, 1295, 1643, 2111
NciI	CCSGG	1 cut(s)	1136
NcoI	CCATGG	1 cut(s)	712
NdeII	GATC	8 cut(s)	111, 617, 991, 1257, 1930, 2163, 2197, 2230
NlaIV	GGNNCC	5 cut(s)	238, 1341, 1708, 2080, 2101
NmeAIII	GCCGAG	1 cut(s)	471
NmuCI	GTSAC	8 cut(s)	191, 730, 841, 1436, 1591, 2169, 2192, 2219
NruI	TCGCGA	1 cut(s)	1018
NspI	RCATGY	2 cut(s)	659, 1693
OliI	CACNNNNGTG	1 cut(s)	939
PaeI	GCATGC	1 cut(s)	659
PaeR7I	CTCGAG	1 cut(s)	568
PauI	GCGCGC	2 cut(s)	1293, 1641
PciSI	GCTCTTC	1 cut(s)	312
PcsI	WCGNNNNNNNCGW	2 cut(s)	1313, 2112
PctI	GAATGC	2 cut(s)	486, 2037
PdmI	GAANNNNTTC	2 cut(s)	1245, 1918
PfeI	GAWTC	4 cut(s)	103, 1005, 1601, 1997
PflFI	GACNNNGTC	1 cut(s)	1442
PflMI	CCANNNNNTGG	1 cut(s)	2094
PfoI	TCCNGGA	1 cut(s)	1134
PkrI	GCNGC	7 cut(s)	205, 418, 823, 964, 1403, 1845, 1926
PleI	GAGTC	2 cut(s)	1544, 1573
PpsI	GAGTC	2 cut(s)	1544, 1573
Psp124BI	GAGCTC	1 cut(s)	324
Psp6I	CCWGG	3 cut(s)	502, 540, 1422
PspFI	CCCAGC	1 cut(s)	679
PspGI	CCWGG	3 cut(s)	502, 540, 1422
PspN4I	GGNNCC	5 cut(s)	238, 1341, 1708, 2080, 2101
PspPI	GGNCC	6 cut(s)	260, 395, 1340, 1607, 1725, 2129
PspXI	VCTCGAGB	1 cut(s)	568
PstI	CTGCAG	1 cut(s)	1101
PstNI	CAGNNNCTG	1 cut(s)	1331
PsuI	RGATCY	1 cut(s)	1257
PsyI	GACNNNGTC	1 cut(s)	1442
PteI	GCGCGC	2 cut(s)	1293, 1641
RruI	TCGCGA	1 cut(s)	1018
RsaI	GTAC	6 cut(s)	1103, 1479, 1793, 1805, 1826, 2101
RsaNI	GTAC	6 cut(s)	1102, 1478, 1792, 1804, 1825, 2100
RseI	CAYNNNNRTG	2 cut(s)	939, 1544
SacI	GAGCTC	1 cut(s)	324
SalI	GTCGAC	1 cut(s)	2105
SapI	GCTCTTC	1 cut(s)	312
SaqAI	TTAA	4 cut(s)	512, 519, 1455, 2030
SatI	GCNGC	7 cut(s)	204, 417, 822, 963, 1402, 1844, 1925
Sau3AI	GATC	8 cut(s)	111, 617, 991, 1257, 1930, 2163, 2197, 2230
Sau96I	GGNCC	6 cut(s)	260, 395, 1340, 1607, 1725, 2129
SchI	GAGTC	2 cut(s)	1544, 1573
ScrFI	CCNGG	4 cut(s)	504, 542, 1136, 1424
SduI	GDGCHC	2 cut(s)	324, 2010
SexAI	ACCWGGT	1 cut(s)	502
SfaNI	GCATC	2 cut(s)	1168, 1909
SfcI	CTRYAG	5 cut(s)	231, 1065, 1097, 1983, 2234
Sfr274I	CTCGAG	1 cut(s)	568
SgrDI	CGTCGACG	1 cut(s)	2105
SinI	GGWCC	5 cut(s)	260, 395, 1607, 1725, 2129
SlaI	CTCGAG	1 cut(s)	568
SmiMI	CAYNNNNRTG	2 cut(s)	939, 1544
SmlI	CTYRAG	2 cut(s)	107, 568
SmoI	CTYRAG	2 cut(s)	107, 568
SphI	GCATGC	1 cut(s)	659
SsiI	CCGC	7 cut(s)	364, 1059, 1397, 1401, 1925, 1967, 2111
SspI	AATATT	3 cut(s)	768, 1486, 1573
SspMI	CTAG	3 cut(s)	167, 299, 1671
SstI	GAGCTC	1 cut(s)	324
StyD4I	CCNGG	4 cut(s)	502, 540, 1134, 1422
StyI	CCWWGG	4 cut(s)	90, 712, 1670, 2088
TaiI	ACGT	4 cut(s)	58, 939, 1593, 2194
TaqII	GACCGA	1 cut(s)	2071
TatI	WGTACW	2 cut(s)	1101, 1791
TauI	GCSGC	2 cut(s)	1404, 1927
TfiI	GAWTC	4 cut(s)	103, 1005, 1601, 1997
Tru1I	TTAA	4 cut(s)	512, 519, 1455, 2030
Tru9I	TTAA	4 cut(s)	512, 519, 1455, 2030
TscAI	CASTG	2 cut(s)	1332, 1999
TseFI	GTSAC	8 cut(s)	191, 730, 841, 1436, 1591, 2169, 2192, 2219
TseI	GCWGC	5 cut(s)	203, 416, 821, 962, 1843
Tsp45I	GTSAC	8 cut(s)	191, 730, 841, 1436, 1591, 2169, 2192, 2219
TspDTI	ATGAA	8 cut(s)	24, 121, 126, 139, 904, 1164, 1392, 1989
TspGWI	ACGGA	2 cut(s)	1198, 1602
TspRI	CASTG	2 cut(s)	1332, 1999
Tth111I	GACNNNGTC	1 cut(s)	1442
Van91I	CCANNNNNTGG	1 cut(s)	2094
VpaK11BI	GGWCC	5 cut(s)	260, 395, 1607, 1725, 2129
XagI	CCTNNNNNAGG	1 cut(s)	1217
XapI	RAATTY	4 cut(s)	871, 1267, 1916, 2070
XceI	RCATGY	2 cut(s)	659, 1693
XhoI	CTCGAG	1 cut(s)	568
XmaJI	CCTAGG	1 cut(s)	1670
XmiI	GTMKAC	2 cut(s)	452, 2106
XmnI	GAANNNNTTC	2 cut(s)	1245, 1918
XspI	CTAG	3 cut(s)	167, 299, 1671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.