FvH4_5g27250

chitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005224
Physical Location & Seq
Forward (+)
18521572 .. 18523643
2072 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27250.t2

Sequence Viewer

Length: 1056 bp
ATGGTGCTCAAATGTAAGAGGGGGGTGTCACTAACATATAATGAACTCCTAAGTTTCGAAGAAGAAAACCATATACATCCTAAATTGGGAATGGGCCGTGGCCCCTTTTGGCTCCGCCACTGCTTGTTGCCGGAGCTGTATGCCGCTGTTTGCAATAACCAATTTGATGGAGTCAACAAATTAAGCAATCAAAACAAACTATCGTCACCTGCAACTCCTAAATCCATGGCTGCAGTCTCGCTGCCATCTTCATCTTCAGTGTTCTCCTTCTTCCTCACTACCATCTTTCTTCTCTCCATATCATCATGGGAAGTTGAGGCTCATCCAGTGCCTGCTTTGATCAATGAAAAGCTTTACAACAACTTGTTTCTACACAAGGACGACACTGCATGCCCTGCAAATAATTTCTACACCTATAGCTCCTTCATCAGGGCAACCAAATACTTCCCGAGATTCGGCACCACCGGAAGTCTAGCCACCCGAAAGCGTGAGATTGCAGCATTTCTTGCTCAGATCTCTCACGAGACCACAGGCGGGTGGGCTACTGCGCCCGACGGACCATACTCGTGGGGTTTGTGCTTTAAAGAGGAGGTCAATCCGGGAAGCAATTACTGCGACGACACGAACAAGGAGTGGCCATGCTATCCTGGAAAATCTTACAAAGGAAGAGGACCGATTCAACTATCTTGGAATTATAATTATGGTCAGGCAGGCAAGGCATTAGGGTTTGATGGACTTAAAAATCCAGAAGTAGTAGCAAACAGTTCCCTAATAGCTTTCAAAACAGCTCTATGGTTCTGGATGACGGAGCAAAAGCCGAAGCCCTCTTGTCACGATGTCATGGTAGGTCGATATGTGCTCACGCAAGCTGACATTGCTGCAAATAGGACTGTCGGATTCGGGATGGTGACGAACATCATCAACGGTGGACTCGAGTGTGGAATAGGCAACGATGCACGAGTAAATGATCGAATTGGCTATTTCAAAAGATATGCTAGTCTGTTTGGTGTTCAAACAGGACCAAATTTAGACTGTGAAAATCAGAAGTCCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_19

Protein Analysis

352

Amino Acids

39.02

Weight (kDa)

8.58

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 68 - 299 7.1e-107 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AarI CACCTGC 1 cut(s) 217
Acc36I ACCTGC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 458
AciI CCGC 3 cut(s) 115, 144, 534
AcoI YGGCCR 1 cut(s) 635
AcsI RAATTY 1 cut(s) 1024
AcuI CTGAAG 1 cut(s) 240
AfiI CCNNNNNNNGG 4 cut(s) 86, 429, 455, 534
AgsI TTSAA 4 cut(s) 680, 781, 985, 1013
AjnI CCWGG 1 cut(s) 646
AluBI AGCT 6 cut(s) 136, 352, 420, 776, 788, 869
AluI AGCT 6 cut(s) 136, 352, 420, 776, 788, 869
Alw21I GWGCWC 2 cut(s) 9, 861
Alw26I GTCTC 2 cut(s) 241, 518
AlwNI CAGNNNCTG 1 cut(s) 332
Ama87I CYCGRG 2 cut(s) 448, 932
AoxI GGCC 3 cut(s) 94, 100, 635
ApeKI GCWGC 4 cut(s) 230, 241, 497, 878
ApoI RAATTY 1 cut(s) 1024
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 5 cut(s) 94, 101, 557, 671, 1019
AsuC2I CCSGG 1 cut(s) 600
AsuHPI GGTGA 2 cut(s) 198, 919
AsuII TTCGAA 1 cut(s) 57
AvaI CYCGRG 2 cut(s) 448, 932
AvaII GGWCC 3 cut(s) 557, 671, 1019
BalI TGGCCA 1 cut(s) 637
BanI GGYRCC 1 cut(s) 458
BauI CACGAG 3 cut(s) 521, 565, 957
Bbv12I GWGCWC 2 cut(s) 9, 861
BbvI GCAGC 4 cut(s) 217, 228, 509, 865
BccI CCATC 5 cut(s) 161, 253, 290, 725, 898
BceAI ACGGC 1 cut(s) 81
BciT130I CCWGG 1 cut(s) 648
BclI TGATCA 1 cut(s) 339
BcnI CCSGG 1 cut(s) 600
BcoDI GTCTC 2 cut(s) 241, 518
BfaI CTAG 2 cut(s) 473, 996
BfmI CTRYAG 2 cut(s) 231, 415
BfuAI ACCTGC 1 cut(s) 217
BglII AGATCT 1 cut(s) 513
BisI GCNGC 5 cut(s) 144, 231, 242, 498, 879
BlsI GCNGC 5 cut(s) 145, 232, 243, 499, 880
Bme1390I CCNGG 2 cut(s) 600, 648
Bme18I GGWCC 3 cut(s) 557, 671, 1019
BmeT110I CYCGRG 2 cut(s) 448, 932
BmgT120I GGNCC 5 cut(s) 94, 101, 557, 671, 1019
BmiI GGNNCC 3 cut(s) 103, 113, 460
BmrFI CCNGG 2 cut(s) 600, 648
BmsI GCATC 1 cut(s) 943
Bpu14I TTCGAA 1 cut(s) 57
BpuMI CCSGG 1 cut(s) 600
BsaI GGTCTC 1 cut(s) 518
BsaJI CCNNGG 2 cut(s) 97, 225
BsaWI WCCGGW 1 cut(s) 464
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 429, 455, 534
Bse1I ACTGG 1 cut(s) 326
Bse3DI GCAATG 1 cut(s) 873
BseBI CCWGG 1 cut(s) 648
BseDI CCNNGG 2 cut(s) 97, 225
BseGI GGATG 4 cut(s) 76, 322, 807, 909
BseLI CCNNNNNNNGG 4 cut(s) 86, 429, 455, 534
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 1 cut(s) 524
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 1 cut(s) 602
BseXI GCAGC 4 cut(s) 217, 228, 509, 865
BshFI GGCC 3 cut(s) 96, 102, 637
BshNI GGYRCC 1 cut(s) 458
BsiHKAI GWGCWC 2 cut(s) 9, 861
BsiHKCI CYCGRG 2 cut(s) 448, 932
BsiSI CCGG 3 cut(s) 131, 465, 599
BslI CCNNNNNNNGG 4 cut(s) 86, 429, 455, 534
BsmAI GTCTC 2 cut(s) 241, 518
BsnI GGCC 3 cut(s) 96, 102, 637
Bso31I GGTCTC 1 cut(s) 518
BsoBI CYCGRG 2 cut(s) 448, 932
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 9, 861
Bsp143I GATC 3 cut(s) 339, 513, 967
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 3 cut(s) 115, 144, 534
BspANI GGCC 3 cut(s) 96, 102, 637
BspCNI CTCAG 1 cut(s) 523
BspLI GGNNCC 3 cut(s) 103, 113, 460
BspMAI CTGCAG 1 cut(s) 235
BspMI ACCTGC 1 cut(s) 217
BspT104I TTCGAA 1 cut(s) 57
BspT107I GGYRCC 1 cut(s) 458
BspTNI GGTCTC 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 873
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 2 cut(s) 97, 225
BssMI GATC 3 cut(s) 339, 513, 967
BssSI CACGAG 3 cut(s) 521, 565, 957
BssT1I CCWWGG 1 cut(s) 225
Bst2BI CACGAG 3 cut(s) 521, 565, 957
Bst2UI CCWGG 1 cut(s) 648
Bst4CI ACNGT 4 cut(s) 764, 892, 926, 1034
Bst6I CTCTTC 1 cut(s) 661
BstAPI GCANNNNNTGC 3 cut(s) 395, 506, 612
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 4 cut(s) 333, 391, 712, 867
BstDEI CTNAG 2 cut(s) 50, 510
BstDSI CCRYGG 2 cut(s) 97, 225
BstENI CCTNNNNNAGG 1 cut(s) 427
BstF5I GGATG 4 cut(s) 76, 322, 807, 909
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 3 cut(s) 342, 516, 970
BstMAI GTCTC 2 cut(s) 241, 518
BstMBI GATC 3 cut(s) 339, 513, 967
BstMWI GCNNNNNNNGC 5 cut(s) 395, 506, 612, 716, 875
BstNI CCWGG 1 cut(s) 648
BstNSI RCATGY 1 cut(s) 393
BstSCI CCNGG 2 cut(s) 598, 646
BstSFI CTRYAG 2 cut(s) 231, 415
BstV1I GCAGC 4 cut(s) 217, 228, 509, 865
BstX2I RGATCY 1 cut(s) 513
BstXI CCANNNNNNTGG 2 cut(s) 167, 567
BstYI RGATCY 1 cut(s) 513
BsuRI GGCC 3 cut(s) 96, 102, 637
BtgI CCRYGG 2 cut(s) 97, 225
BtsCI GGATG 4 cut(s) 76, 322, 807, 909
BtsI GCAGTG 2 cut(s) 118, 384
BtsIMutI CAGTG 4 cut(s) 118, 264, 333, 384
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 4 cut(s) 333, 391, 712, 867
CaiI CAGNNNCTG 1 cut(s) 332
CfoI GCGC 1 cut(s) 550
Cfr13I GGNCC 5 cut(s) 94, 101, 557, 671, 1019
CviAII CATG 5 cut(s) 226, 306, 390, 639, 841
DdeI CTNAG 2 cut(s) 50, 510
DpnI GATC 3 cut(s) 341, 515, 969
DpnII GATC 3 cut(s) 339, 513, 967
DraI TTTAAA 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 635
Eam1104I CTCTTC 1 cut(s) 661
EarI CTCTTC 1 cut(s) 661
EciI GGCGGA 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 518
Eco47I GGWCC 3 cut(s) 557, 671, 1019
Eco57I CTGAAG 1 cut(s) 240
Eco88I CYCGRG 2 cut(s) 448, 932
EcoNI CCTNNNNNAGG 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 646
EcoT14I CCWWGG 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 5 cut(s) 229, 309, 393, 642, 844
FatI CATG 5 cut(s) 225, 305, 389, 638, 840
FauI CCCGC 1 cut(s) 527
FbaI TGATCA 1 cut(s) 339
Fnu4HI GCNGC 5 cut(s) 144, 231, 242, 498, 879
FokI GGATG 4 cut(s) 63, 309, 814, 916
Fsp4HI GCNGC 5 cut(s) 144, 231, 242, 498, 879
FspBI CTAG 2 cut(s) 473, 996
GlaI GCGC 1 cut(s) 549
GluI GCNGC 5 cut(s) 144, 231, 242, 498, 879
HaeIII GGCC 3 cut(s) 96, 102, 637
HapII CCGG 3 cut(s) 131, 465, 599
HhaI GCGC 1 cut(s) 550
Hin1II CATG 5 cut(s) 229, 309, 393, 642, 844
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 5 cut(s) 171, 453, 676, 897, 930
HpaII CCGG 3 cut(s) 131, 465, 599
HphI GGTGA 2 cut(s) 198, 919
Hpy166II GTNNAC 2 cut(s) 175, 929
Hpy188I TCNGA 3 cut(s) 513, 896, 1044
Hpy188III TCNNGA 6 cut(s) 448, 521, 746, 799, 833, 901
Hpy8I GTNNAC 2 cut(s) 175, 929
Hpy99I CGWCG 2 cut(s) 557, 620
HpyAV CCTTC 2 cut(s) 277, 433
HpyCH4III ACNGT 4 cut(s) 764, 892, 926, 1034
HpyCH4V TGCA 8 cut(s) 153, 212, 233, 389, 398, 497, 881, 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 395, 506, 612, 716, 875
HpyF3I CTNAG 2 cut(s) 50, 510
Hsp92II CATG 5 cut(s) 229, 309, 393, 642, 844
HspAI GCGC 1 cut(s) 548
Ksp22I TGATCA 1 cut(s) 339
Kzo9I GATC 3 cut(s) 339, 513, 967
LmnI GCTCC 4 cut(s) 117, 133, 425, 808
Lsp1109I GCAGC 4 cut(s) 217, 228, 509, 865
LweI GCATC 1 cut(s) 943
MaeI CTAG 2 cut(s) 473, 996
MaeIII GTNAC 4 cut(s) 27, 204, 830, 907
MalI GATC 3 cut(s) 341, 515, 969
MboI GATC 3 cut(s) 339, 513, 967
MboII GAAGA 7 cut(s) 71, 74, 240, 246, 262, 281, 678
MflI RGATCY 1 cut(s) 513
MhlI GDGCHC 2 cut(s) 9, 861
MlsI TGGCCA 1 cut(s) 637
MluCI AATT 9 cut(s) 83, 161, 179, 403, 607, 691, 697, 972, 1024
MluNI TGGCCA 1 cut(s) 637
MlyI GAGTC 2 cut(s) 180, 924
MmeI TCCRAC 1 cut(s) 874
MnlI CCTC 7 cut(s) 12, 284, 310, 580, 583, 662, 835
Mox20I TGGCCA 1 cut(s) 637
MscI TGGCCA 1 cut(s) 637
MseI TTAA 3 cut(s) 182, 582, 738
MslI CAYNNNNRTG 1 cut(s) 565
Msp20I TGGCCA 1 cut(s) 637
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 3 cut(s) 131, 465, 599
MspR9I CCNGG 2 cut(s) 600, 648
MvaI CCWGG 1 cut(s) 648
MwoI GCNNNNNNNGC 5 cut(s) 395, 506, 612, 716, 875
NciI CCSGG 1 cut(s) 600
NcoI CCATGG 1 cut(s) 225
NdeII GATC 3 cut(s) 339, 513, 967
NlaIII CATG 5 cut(s) 229, 309, 393, 642, 844
NlaIV GGNNCC 3 cut(s) 103, 113, 460
NmuCI GTSAC 4 cut(s) 27, 204, 830, 907
NspI RCATGY 1 cut(s) 393
NspV TTCGAA 1 cut(s) 57
PaeI GCATGC 1 cut(s) 393
PaeR7I CTCGAG 1 cut(s) 932
PaqCI CACCTGC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 930
PfeI GAWTC 3 cut(s) 453, 676, 897
PfoI TCCNGGA 2 cut(s) 598, 646
PkrI GCNGC 5 cut(s) 145, 232, 243, 499, 880
PleI GAGTC 2 cut(s) 179, 924
PpsI GAGTC 2 cut(s) 179, 924
PsiI TTATAA 1 cut(s) 696
Psp6I CCWGG 1 cut(s) 646
PspGI CCWGG 1 cut(s) 646
PspN4I GGNNCC 3 cut(s) 103, 113, 460
PspPI GGNCC 5 cut(s) 94, 101, 557, 671, 1019
PspXI VCTCGAGB 1 cut(s) 932
PstI CTGCAG 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 1 cut(s) 513
RseI CAYNNNNRTG 1 cut(s) 565
SaqAI TTAA 3 cut(s) 182, 582, 738
SatI GCNGC 5 cut(s) 144, 231, 242, 498, 879
Sau3AI GATC 3 cut(s) 339, 513, 967
Sau96I GGNCC 5 cut(s) 94, 101, 557, 671, 1019
SchI GAGTC 2 cut(s) 180, 924
ScrFI CCNGG 2 cut(s) 600, 648
SduI GDGCHC 2 cut(s) 9, 861
SfaNI GCATC 1 cut(s) 943
SfcI CTRYAG 2 cut(s) 231, 415
Sfr274I CTCGAG 1 cut(s) 932
SfuI TTCGAA 1 cut(s) 57
SinI GGWCC 3 cut(s) 557, 671, 1019
SlaI CTCGAG 1 cut(s) 932
SmiMI CAYNNNNRTG 1 cut(s) 565
SmlI CTYRAG 1 cut(s) 932
SmoI CTYRAG 1 cut(s) 932
SphI GCATGC 1 cut(s) 393
Sse9I AATT 9 cut(s) 83, 161, 179, 403, 607, 691, 697, 972, 1024
SsiI CCGC 3 cut(s) 115, 144, 534
SspMI CTAG 2 cut(s) 473, 996
StyD4I CCNGG 2 cut(s) 598, 646
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 4 cut(s) 764, 892, 926, 1034
TaqI TCGA 4 cut(s) 57, 850, 933, 970
TaqII GACCGA 1 cut(s) 688
TasI AATT 9 cut(s) 83, 161, 179, 403, 607, 691, 697, 972, 1024
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 3 cut(s) 453, 676, 897
Tru1I TTAA 3 cut(s) 182, 582, 738
Tru9I TTAA 3 cut(s) 182, 582, 738
TscAI CASTG 4 cut(s) 125, 264, 333, 391
TseFI GTSAC 4 cut(s) 27, 204, 830, 907
TseI GCWGC 4 cut(s) 230, 241, 497, 878
Tsp45I GTSAC 4 cut(s) 27, 204, 830, 907
TspDTI ATGAA 4 cut(s) 57, 240, 360, 415
TspGWI ACGGA 2 cut(s) 570, 821
TspRI CASTG 4 cut(s) 125, 264, 333, 391
VpaK11BI GGWCC 3 cut(s) 557, 671, 1019
XagI CCTNNNNNAGG 1 cut(s) 427
XapI RAATTY 1 cut(s) 1024
XceI RCATGY 1 cut(s) 393
XhoI CTCGAG 1 cut(s) 932
XspI CTAG 2 cut(s) 473, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.