RLG00000002373

chitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005224
Physical Location & Seq
Reverse (-)
32995709 .. 32996703
995 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002373

Sequence Viewer

Length: 834 bp
ATGGCTGCGGTCTTGCTGCTGTCACCTTCAGCCTTCTTCTTCTTCTTCTTCCTCACTACCATCTTTCTTTTCTCCATCTCATCATGGGAAGTTGAGGCTCATCCAGTATCTACTTTGATCAACAAAAAGCTTTACAACAACTTGTTTCTGCATAAGGACGACACAGCATGCCCTGCAAATGATTTCTACACTTATCGCTCCTTCATCGAGGCAACCAAATATTTTCCGAGATTCGGCACCACCGGAAGTCTAGCCACTCGAAAGCGTGAAATTGCAGCATTTCTTGCTCAGATCTCTCACGAGACCACAGGCGGGTGGGCTACAGCGCCCGACGGACCATACTCTTGGGGTTTGTGCTTTAAAGAGGAAGTCAATCCGGCAAGCAACTATTGCGACAGCACCAACAAGGAGTGGCCATGCTATCCTGGAAAATCTTACAAAGGAAGAGGACCAATTCAACTATCTTGGAACTATAATTATGGTCAGGCAGGCAAGGCATTAAGGTTTGATGGACTAAAAAATCCAGAAGTAGTAGCAAACAATTCCTTAATAGCTTTCAAAACAGCTATATGGTTCTGGATGACTGAGCAAAAGCCGAAGCCCTCTTGTCACGACGTCATGGTGGGTCGATATGTGCTCACGCAAGCTGACATGGCTGCTAATAGGACTGTCGGATTCGGGATGGTGACGAACATCATCAACGGTGGACTTGAGTGTGGAATAGGCAGCGATGCACGAGTGAATGATCGAATTGGTTATTTCAAAAGATATGCTAGTCTGTTTGGTGTTCAAACAGGACCAAATTTAGACTGTGAAAATCAAAAGTCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_19

Protein Analysis

278

Amino Acids

31.06

Weight (kDa)

8.59

Isoelectric Point (pI)

30.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 39 - 271 2e-106 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 618
AccB1I GGYRCC 1 cut(s) 236
AciI CCGC 2 cut(s) 8, 312
AcoI YGGCCR 1 cut(s) 413
AcsI RAATTY 1 cut(s) 802
AcuI CTGAAG 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 615
AfiI CCNNNNNNNGG 2 cut(s) 233, 312
AgsI TTSAA 4 cut(s) 458, 559, 763, 791
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 4 cut(s) 130, 554, 566, 647
AluI AGCT 4 cut(s) 130, 554, 566, 647
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 1 cut(s) 296
AoxI GGCC 1 cut(s) 413
ApeKI GCWGC 5 cut(s) 5, 16, 275, 656, 726
ApoI RAATTY 1 cut(s) 802
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 3 cut(s) 335, 449, 797
AsuHPI GGTGA 2 cut(s) 15, 697
AvaII GGWCC 3 cut(s) 335, 449, 797
BalI TGGCCA 1 cut(s) 415
BanI GGYRCC 1 cut(s) 236
BauI CACGAG 2 cut(s) 299, 735
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 4 cut(s) 3, 287, 643, 738
BccI CCATC 4 cut(s) 68, 83, 503, 676
BciT130I CCWGG 1 cut(s) 426
BclI TGATCA 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 2 cut(s) 251, 774
BfmI CTRYAG 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 329
BglII AGATCT 1 cut(s) 291
BisI GCNGC 5 cut(s) 6, 17, 276, 657, 727
BlsI GCNGC 5 cut(s) 7, 18, 277, 658, 728
Bme1390I CCNGG 1 cut(s) 426
Bme18I GGWCC 3 cut(s) 335, 449, 797
BmgT120I GGNCC 3 cut(s) 335, 449, 797
BmiI GGNNCC 1 cut(s) 238
BmrFI CCNGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 721
BpuEI CTTGAG 1 cut(s) 731
BsaHI GRCGYC 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 296
BsaWI WCCGGW 1 cut(s) 242
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 312
Bse1I ACTGG 1 cut(s) 104
BseBI CCWGG 1 cut(s) 426
BseGI GGATG 3 cut(s) 100, 585, 687
BseLI CCNNNNNNNGG 2 cut(s) 233, 312
BseMII CTCAG 2 cut(s) 302, 576
BseNI ACTGG 1 cut(s) 104
BseXI GCAGC 4 cut(s) 3, 287, 643, 738
BshFI GGCC 1 cut(s) 415
BshNI GGYRCC 1 cut(s) 236
BsiHKAI GWGCWC 1 cut(s) 639
BsiSI CCGG 2 cut(s) 243, 377
BslI CCNNNNNNNGG 2 cut(s) 233, 312
BsmAI GTCTC 1 cut(s) 296
BsnI GGCC 1 cut(s) 415
Bso31I GGTCTC 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 639
Bsp143I GATC 3 cut(s) 117, 291, 745
BspACI CCGC 2 cut(s) 8, 312
BspANI GGCC 1 cut(s) 415
BspCNI CTCAG 2 cut(s) 301, 577
BspLI GGNNCC 1 cut(s) 238
BspT107I GGYRCC 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 296
BsrI ACTGG 1 cut(s) 104
BssMI GATC 3 cut(s) 117, 291, 745
BssNI GRCGYC 1 cut(s) 615
BssSI CACGAG 2 cut(s) 299, 735
Bst2BI CACGAG 2 cut(s) 299, 735
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 3 cut(s) 670, 704, 812
Bst6I CTCTTC 1 cut(s) 439
BstACI GRCGYC 1 cut(s) 615
BstAPI GCANNNNNTGC 3 cut(s) 173, 284, 390
BstC8I GCNNGC 4 cut(s) 169, 382, 490, 645
BstDEI CTNAG 2 cut(s) 288, 585
BstF5I GGATG 3 cut(s) 100, 585, 687
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 3 cut(s) 120, 294, 748
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 3 cut(s) 117, 291, 745
BstMWI GCNNNNNNNGC 5 cut(s) 173, 284, 390, 494, 653
BstNI CCWGG 1 cut(s) 426
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 1 cut(s) 321
BstV1I GCAGC 4 cut(s) 3, 287, 643, 738
BstX2I RGATCY 1 cut(s) 291
BstXI CCANNNNNNTGG 1 cut(s) 345
BstYI RGATCY 1 cut(s) 291
BsuRI GGCC 1 cut(s) 415
BtgZI GCGATG 1 cut(s) 744
BtsCI GGATG 3 cut(s) 100, 585, 687
Cac8I GCNNGC 4 cut(s) 169, 382, 490, 645
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 335, 449, 797
CviAII CATG 5 cut(s) 84, 168, 417, 619, 652
DdeI CTNAG 2 cut(s) 288, 585
DpnI GATC 3 cut(s) 119, 293, 747
DpnII GATC 3 cut(s) 117, 291, 745
DraI TTTAAA 1 cut(s) 361
EaeI YGGCCR 1 cut(s) 413
Eam1104I CTCTTC 1 cut(s) 439
EarI CTCTTC 1 cut(s) 439
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 3 cut(s) 335, 449, 797
Eco57I CTGAAG 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 424
FaeI CATG 5 cut(s) 87, 171, 420, 622, 655
FatI CATG 5 cut(s) 83, 167, 416, 618, 651
FauI CCCGC 1 cut(s) 305
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 5 cut(s) 6, 17, 276, 657, 727
FokI GGATG 3 cut(s) 87, 592, 694
Fsp4HI GCNGC 5 cut(s) 6, 17, 276, 657, 727
FspBI CTAG 2 cut(s) 251, 774
GlaI GCGC 1 cut(s) 327
GluI GCNGC 5 cut(s) 6, 17, 276, 657, 727
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 1 cut(s) 415
HapII CCGG 2 cut(s) 243, 377
HhaI GCGC 1 cut(s) 328
Hin1I GRCGYC 1 cut(s) 615
Hin1II CATG 5 cut(s) 87, 171, 420, 622, 655
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 2 cut(s) 231, 675
HpaII CCGG 2 cut(s) 243, 377
HphI GGTGA 2 cut(s) 15, 697
Hpy166II GTNNAC 1 cut(s) 707
Hpy188I TCNGA 3 cut(s) 228, 291, 674
Hpy188III TCNNGA 5 cut(s) 299, 524, 577, 611, 679
Hpy8I GTNNAC 1 cut(s) 707
Hpy99I CGWCG 2 cut(s) 335, 617
HpyAV CCTTC 3 cut(s) 36, 43, 211
HpyCH4III ACNGT 3 cut(s) 670, 704, 812
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 4 cut(s) 151, 176, 275, 734
HpyF10VI GCNNNNNNNGC 5 cut(s) 173, 284, 390, 494, 653
HpyF3I CTNAG 2 cut(s) 288, 585
HpySE526I ACGT 1 cut(s) 615
Hsp92I GRCGYC 1 cut(s) 615
Hsp92II CATG 5 cut(s) 87, 171, 420, 622, 655
HspAI GCGC 1 cut(s) 326
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 117, 291, 745
LmnI GCTCC 1 cut(s) 203
Lsp1109I GCAGC 4 cut(s) 3, 287, 643, 738
LweI GCATC 1 cut(s) 721
MaeI CTAG 2 cut(s) 251, 774
MaeII ACGT 1 cut(s) 615
MaeIII GTNAC 3 cut(s) 21, 608, 685
MalI GATC 3 cut(s) 119, 293, 747
MboI GATC 3 cut(s) 117, 291, 745
MboII GAAGA 6 cut(s) 28, 31, 34, 37, 40, 456
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 639
MlsI TGGCCA 1 cut(s) 415
MluCI AATT 6 cut(s) 270, 453, 475, 541, 750, 802
MluNI TGGCCA 1 cut(s) 415
MmeI TCCRAC 1 cut(s) 652
MnlI CCTC 6 cut(s) 62, 88, 202, 358, 440, 613
Mox20I TGGCCA 1 cut(s) 415
MscI TGGCCA 1 cut(s) 415
MseI TTAA 4 cut(s) 360, 500, 548, 832
Msp20I TGGCCA 1 cut(s) 415
MspI CCGG 2 cut(s) 243, 377
MspR9I CCNGG 1 cut(s) 426
MvaI CCWGG 1 cut(s) 426
MwoI GCNNNNNNNGC 5 cut(s) 173, 284, 390, 494, 653
NdeII GATC 3 cut(s) 117, 291, 745
NlaIII CATG 5 cut(s) 87, 171, 420, 622, 655
NlaIV GGNNCC 1 cut(s) 238
NmuCI GTSAC 3 cut(s) 21, 608, 685
NspI RCATGY 1 cut(s) 171
PaeI GCATGC 1 cut(s) 171
PfeI GAWTC 2 cut(s) 231, 675
PfoI TCCNGGA 1 cut(s) 424
PkrI GCNGC 5 cut(s) 7, 18, 277, 658, 728
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 238
PspPI GGNCC 3 cut(s) 335, 449, 797
PsuI RGATCY 1 cut(s) 291
SaqAI TTAA 4 cut(s) 360, 500, 548, 832
SatI GCNGC 5 cut(s) 6, 17, 276, 657, 727
Sau3AI GATC 3 cut(s) 117, 291, 745
Sau96I GGNCC 3 cut(s) 335, 449, 797
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 1 cut(s) 639
SetI ASST 7 cut(s) 28, 132, 506, 556, 568, 618, 649
SfaNI GCATC 1 cut(s) 721
SfcI CTRYAG 1 cut(s) 321
SinI GGWCC 3 cut(s) 335, 449, 797
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
SphI GCATGC 1 cut(s) 171
Sse9I AATT 6 cut(s) 270, 453, 475, 541, 750, 802
SsiI CCGC 2 cut(s) 8, 312
SspI AATATT 1 cut(s) 221
SspMI CTAG 2 cut(s) 251, 774
StyD4I CCNGG 1 cut(s) 424
TaaI ACNGT 3 cut(s) 670, 704, 812
TaiI ACGT 1 cut(s) 618
TaqI TCGA 4 cut(s) 207, 259, 628, 748
TasI AATT 6 cut(s) 270, 453, 475, 541, 750, 802
TfiI GAWTC 2 cut(s) 231, 675
Tru1I TTAA 4 cut(s) 360, 500, 548, 832
Tru9I TTAA 4 cut(s) 360, 500, 548, 832
TseFI GTSAC 3 cut(s) 21, 608, 685
TseI GCWGC 5 cut(s) 5, 16, 275, 656, 726
Tsp45I GTSAC 3 cut(s) 21, 608, 685
TspDTI ATGAA 1 cut(s) 193
TspGWI ACGGA 1 cut(s) 348
VpaK11BI GGWCC 3 cut(s) 335, 449, 797
XapI RAATTY 1 cut(s) 802
XceI RCATGY 1 cut(s) 171
XspI CTAG 2 cut(s) 251, 774
ZraI GACGTC 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.