RLG00000023134

chitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005224
Physical Location & Seq
Forward (+)
18678633 .. 18679779
1147 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023134

Sequence Viewer

Length: 825 bp
ATGGCTGCGGTCTCGCTGTCATCTGCAGTCTTCCTCTTCTCCATCACTACCATCTTTCTTGTCTCCATCTCATCATGGGAAGCTGAGGCTCATCCAGTATCTACTTTGATCAAGAAAAAGCTTTACAAAAACTTGTTTCTACACAAGGACGACAAGGCATGCCCTGCAAATGACTTCTATACCTACCGCTCCTTCATCAAGGCAACCAAATTCTTCCCGGAATTCGGCACCACTGGAAGTCTAGCCACCCGAAAGCGTGAGATTGCAGCATTTCTTGCTCAGATCTCTCACGAGACCACGGGAGGCTGGGCTACTGCGCCCGACGGACCATATTCGTGGGGTTTGTGCTATAAAGAGGAAATCAGTCCGACCAGCAATTACTGTGATGATACCAACAAAGAGTGGCCATGCTATCCGGGCAAATCTTACAAAGGAAGAGGACCAGTTCAATTATCTTGGAACTATAATTATGGCCAGGCAGGCAAGGCATTAGGGTTTGATGGGCTAAAGAATCCAGAATTAGTAGCAAACAGTTCCATCATCGCATTCAAAACGGCTTTATGGTTCTGGATGACTGAGCAAAAGCCGAAGCCCTCATGCCACGATGTCATGGTAGGCCGATATGCGCAAACGCAAGCTGATGTGGCGGCGAATAGGACTGCCGGGTTCGGGATGGTGACGAACATCATCAACGGTGAGCTCGAGTGCGGAATAGGTAGCGATGCACGAGTGAATGATCGAATTGGTTATTTCAAAAGATATGCTAGTCTGTTTAGTGTTCAGACCGGGCCAAATTTAGATTGTGAAAATCAAAAATCCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_19

Protein Analysis

275

Amino Acids

30.43

Weight (kDa)

8.56

Isoelectric Point (pI)

29.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 36 - 268 6.3e-107 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 627
AccB1I GGYRCC 1 cut(s) 227
AccBSI CCGCTC 1 cut(s) 189
AciI CCGC 4 cut(s) 8, 187, 647, 708
AcoI YGGCCR 2 cut(s) 404, 472
AcsI RAATTY 3 cut(s) 209, 221, 793
AfiI CCNNNNNNNGG 2 cut(s) 224, 669
AgsI TTSAA 3 cut(s) 449, 550, 754
AjnI CCWGG 1 cut(s) 474
AluBI AGCT 4 cut(s) 83, 121, 638, 700
AluI AGCT 4 cut(s) 83, 121, 638, 700
Alw21I GWGCWC 1 cut(s) 702
Alw26I GTCTC 3 cut(s) 16, 67, 287
Ama87I CYCGRG 1 cut(s) 701
AoxI GGCC 4 cut(s) 404, 472, 616, 788
ApeKI GCWGC 2 cut(s) 5, 266
ApoI RAATTY 3 cut(s) 209, 221, 793
AspLEI GCGC 2 cut(s) 319, 628
AspS9I GGNCC 3 cut(s) 326, 440, 788
AsuC2I CCSGG 4 cut(s) 218, 417, 664, 787
AsuHPI GGTGA 2 cut(s) 688, 707
AvaI CYCGRG 1 cut(s) 701
AvaII GGWCC 2 cut(s) 326, 440
BalI TGGCCA 2 cut(s) 406, 474
BanI GGYRCC 1 cut(s) 227
BanII GRGCYC 1 cut(s) 702
BauI CACGAG 2 cut(s) 290, 726
BbsI GAAGAC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 702
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 278
BccI CCATC 6 cut(s) 50, 59, 74, 494, 545, 667
BceAI ACGGC 1 cut(s) 570
BciT130I CCWGG 1 cut(s) 476
BclI TGATCA 1 cut(s) 108
BcnI CCSGG 4 cut(s) 218, 417, 664, 787
BcoDI GTCTC 3 cut(s) 16, 67, 287
BfaI CTAG 2 cut(s) 242, 765
BfmI CTRYAG 1 cut(s) 24
BglI GCCNNNNNGGC 1 cut(s) 480
BglII AGATCT 1 cut(s) 282
BisI GCNGC 3 cut(s) 6, 267, 648
BlsI GCNGC 3 cut(s) 7, 268, 649
Bme1390I CCNGG 5 cut(s) 218, 417, 476, 664, 787
Bme18I GGWCC 2 cut(s) 326, 440
BmeT110I CYCGRG 1 cut(s) 701
BmgT120I GGNCC 3 cut(s) 326, 440, 788
BmiI GGNNCC 1 cut(s) 229
BmrFI CCNGG 5 cut(s) 218, 417, 476, 664, 787
BmsI GCATC 1 cut(s) 712
BpiI GAAGAC 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 84
BpuMI CCSGG 4 cut(s) 218, 417, 664, 787
BsaI GGTCTC 2 cut(s) 16, 287
BsaJI CCNNGG 1 cut(s) 297
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 669
Bse1I ACTGG 3 cut(s) 95, 238, 443
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 1 cut(s) 297
BseGI GGATG 3 cut(s) 91, 576, 678
BseLI CCNNNNNNNGG 2 cut(s) 224, 669
BseMII CTCAG 3 cut(s) 75, 293, 567
BseNI ACTGG 3 cut(s) 95, 238, 443
BseXI GCAGC 1 cut(s) 278
BseYI CCCAGC 1 cut(s) 306
BshFI GGCC 4 cut(s) 406, 474, 618, 790
BshNI GGYRCC 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 702
BsiHKCI CYCGRG 1 cut(s) 701
BsiSI CCGG 4 cut(s) 218, 416, 663, 786
BslI CCNNNNNNNGG 2 cut(s) 224, 669
BsmAI GTCTC 3 cut(s) 16, 67, 287
BsmI GAATGC 1 cut(s) 545
BsnI GGCC 4 cut(s) 406, 474, 618, 790
Bso31I GGTCTC 2 cut(s) 16, 287
BsoBI CYCGRG 1 cut(s) 701
Bsp1286I GDGCHC 1 cut(s) 702
Bsp143I GATC 3 cut(s) 108, 282, 736
BspACI CCGC 4 cut(s) 8, 187, 647, 708
BspANI GGCC 4 cut(s) 406, 474, 618, 790
BspCNI CTCAG 3 cut(s) 76, 292, 568
BspLI GGNNCC 1 cut(s) 229
BspMAI CTGCAG 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 227
BspTNI GGTCTC 2 cut(s) 16, 287
BsrBI CCGCTC 1 cut(s) 189
BsrI ACTGG 3 cut(s) 95, 238, 443
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 3 cut(s) 108, 282, 736
BssSI CACGAG 2 cut(s) 290, 726
Bst2BI CACGAG 2 cut(s) 290, 726
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 3 cut(s) 383, 533, 695
Bst6I CTCTTC 2 cut(s) 41, 430
BstAPI GCANNNNNTGC 2 cut(s) 164, 275
BstC8I GCNNGC 3 cut(s) 160, 481, 636
BstDEI CTNAG 3 cut(s) 84, 279, 576
BstDSI CCRYGG 1 cut(s) 297
BstF5I GGATG 3 cut(s) 91, 576, 678
BstHHI GCGC 2 cut(s) 319, 628
BstKTI GATC 3 cut(s) 111, 285, 739
BstMAI GTCTC 3 cut(s) 16, 67, 287
BstMBI GATC 3 cut(s) 108, 282, 736
BstMWI GCNNNNNNNGC 6 cut(s) 164, 275, 417, 480, 485, 644
BstNI CCWGG 1 cut(s) 476
BstNSI RCATGY 1 cut(s) 162
BstSCI CCNGG 5 cut(s) 216, 415, 474, 662, 785
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 278
BstV2I GAAGAC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 282
BstXI CCANNNNNNTGG 1 cut(s) 336
BstYI RGATCY 1 cut(s) 282
BsuRI GGCC 4 cut(s) 406, 474, 618, 790
BtgI CCRYGG 1 cut(s) 297
BtgZI GCGATG 2 cut(s) 526, 735
BtsCI GGATG 3 cut(s) 91, 576, 678
BtsIMutI CAGTG 1 cut(s) 231
Cac8I GCNNGC 3 cut(s) 160, 481, 636
CfoI GCGC 2 cut(s) 319, 628
Cfr13I GGNCC 3 cut(s) 326, 440, 788
CviAII CATG 5 cut(s) 75, 159, 408, 597, 610
DdeI CTNAG 3 cut(s) 84, 279, 576
DpnI GATC 3 cut(s) 110, 284, 738
DpnII GATC 3 cut(s) 108, 282, 736
EaeI YGGCCR 2 cut(s) 404, 472
Eam1104I CTCTTC 2 cut(s) 41, 430
EarI CTCTTC 2 cut(s) 41, 430
Ecl136II GAGCTC 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 702
Eco31I GGTCTC 2 cut(s) 16, 287
Eco47I GGWCC 2 cut(s) 326, 440
Eco53kI GAGCTC 1 cut(s) 700
Eco88I CYCGRG 1 cut(s) 701
EcoICRI GAGCTC 1 cut(s) 700
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 474
EcoT38I GRGCYC 1 cut(s) 702
FaeI CATG 5 cut(s) 78, 162, 411, 600, 613
FatI CATG 5 cut(s) 74, 158, 407, 596, 609
FbaI TGATCA 1 cut(s) 108
Fnu4HI GCNGC 3 cut(s) 6, 267, 648
FokI GGATG 3 cut(s) 78, 583, 685
FriOI GRGCYC 1 cut(s) 702
Fsp4HI GCNGC 3 cut(s) 6, 267, 648
FspBI CTAG 2 cut(s) 242, 765
FspI TGCGCA 1 cut(s) 627
GlaI GCGC 2 cut(s) 318, 627
GluI GCNGC 3 cut(s) 6, 267, 648
GsaI CCCAGC 1 cut(s) 310
HaeIII GGCC 4 cut(s) 406, 474, 618, 790
HapII CCGG 4 cut(s) 218, 416, 663, 786
HhaI GCGC 2 cut(s) 319, 628
Hin1II CATG 5 cut(s) 78, 162, 411, 600, 613
Hin6I GCGC 2 cut(s) 317, 626
HinP1I GCGC 2 cut(s) 317, 626
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 1 cut(s) 511
HpaII CCGG 4 cut(s) 218, 416, 663, 786
HphI GGTGA 2 cut(s) 688, 707
Hpy188I TCNGA 3 cut(s) 282, 369, 783
Hpy188III TCNNGA 5 cut(s) 112, 290, 515, 568, 670
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 1 cut(s) 202
HpyCH4III ACNGT 3 cut(s) 383, 533, 695
HpyCH4V TGCA 4 cut(s) 26, 167, 266, 725
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 275, 417, 480, 485, 644
HpyF3I CTNAG 3 cut(s) 84, 279, 576
Hsp92II CATG 5 cut(s) 78, 162, 411, 600, 613
HspAI GCGC 2 cut(s) 317, 626
Ksp22I TGATCA 1 cut(s) 108
Kzo9I GATC 3 cut(s) 108, 282, 736
LmnI GCTCC 1 cut(s) 194
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 1 cut(s) 712
MaeI CTAG 2 cut(s) 242, 765
MaeIII GTNAC 1 cut(s) 676
MalI GATC 3 cut(s) 110, 284, 738
MbiI CCGCTC 1 cut(s) 189
MboI GATC 3 cut(s) 108, 282, 736
MboII GAAGA 4 cut(s) 22, 28, 205, 447
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 702
MlsI TGGCCA 2 cut(s) 406, 474
MluCI AATT 8 cut(s) 209, 221, 376, 449, 466, 518, 741, 793
MluNI TGGCCA 2 cut(s) 406, 474
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 6 cut(s) 44, 79, 296, 349, 431, 604
Mox20I TGGCCA 2 cut(s) 406, 474
MscI TGGCCA 2 cut(s) 406, 474
MseI TTAA 1 cut(s) 823
MslI CAYNNNNRTG 1 cut(s) 334
Msp20I TGGCCA 2 cut(s) 406, 474
MspI CCGG 4 cut(s) 218, 416, 663, 786
MspR9I CCNGG 5 cut(s) 218, 417, 476, 664, 787
Mva1269I GAATGC 1 cut(s) 545
MvaI CCWGG 1 cut(s) 476
MwoI GCNNNNNNNGC 6 cut(s) 164, 275, 417, 480, 485, 644
NciI CCSGG 4 cut(s) 218, 417, 664, 787
NdeII GATC 3 cut(s) 108, 282, 736
NlaIII CATG 5 cut(s) 78, 162, 411, 600, 613
NlaIV GGNNCC 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 676
NsbI TGCGCA 1 cut(s) 627
NspI RCATGY 1 cut(s) 162
PaeI GCATGC 1 cut(s) 162
PaeR7I CTCGAG 1 cut(s) 701
PcsI WCGNNNNNNNCGW 1 cut(s) 699
PctI GAATGC 1 cut(s) 545
PfeI GAWTC 1 cut(s) 511
PfoI TCCNGGA 1 cut(s) 216
PkrI GCNGC 3 cut(s) 7, 268, 649
Psp124BI GAGCTC 1 cut(s) 702
Psp6I CCWGG 1 cut(s) 474
PspFI CCCAGC 1 cut(s) 306
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 1 cut(s) 229
PspPI GGNCC 3 cut(s) 326, 440, 788
PspXI VCTCGAGB 1 cut(s) 701
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 1 cut(s) 282
RseI CAYNNNNRTG 1 cut(s) 334
SacI GAGCTC 1 cut(s) 702
SaqAI TTAA 1 cut(s) 823
SatI GCNGC 3 cut(s) 6, 267, 648
Sau3AI GATC 3 cut(s) 108, 282, 736
Sau96I GGNCC 3 cut(s) 326, 440, 788
ScrFI CCNGG 5 cut(s) 218, 417, 476, 664, 787
SduI GDGCHC 1 cut(s) 702
SetI ASST 6 cut(s) 85, 123, 185, 640, 702, 718
SfaNI GCATC 1 cut(s) 712
SfcI CTRYAG 1 cut(s) 24
Sfr274I CTCGAG 1 cut(s) 701
SinI GGWCC 2 cut(s) 326, 440
SlaI CTCGAG 1 cut(s) 701
SmiMI CAYNNNNRTG 1 cut(s) 334
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
SphI GCATGC 1 cut(s) 162
Sse9I AATT 8 cut(s) 209, 221, 376, 449, 466, 518, 741, 793
SsiI CCGC 4 cut(s) 8, 187, 647, 708
SspMI CTAG 2 cut(s) 242, 765
SstI GAGCTC 1 cut(s) 702
StyD4I CCNGG 5 cut(s) 216, 415, 474, 662, 785
TaaI ACNGT 3 cut(s) 383, 533, 695
TaqI TCGA 2 cut(s) 702, 739
TasI AATT 8 cut(s) 209, 221, 376, 449, 466, 518, 741, 793
TauI GCSGC 1 cut(s) 650
TfiI GAWTC 1 cut(s) 511
Tru1I TTAA 1 cut(s) 823
Tru9I TTAA 1 cut(s) 823
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 2 cut(s) 5, 266
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 1 cut(s) 184
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 326, 440
XapI RAATTY 3 cut(s) 209, 221, 793
XceI RCATGY 1 cut(s) 162
XhoI CTCGAG 1 cut(s) 701
XspI CTAG 2 cut(s) 242, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.