Rroxscaffold_3G00240970

chitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005224
Physical Location & Seq
Reverse (-)
32951983 .. 32953326
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00240970.1

Sequence Viewer

Length: 831 bp
ATGGCTGCGGTCTTGCTGTCATCTTCAGCCTTCTTCTTCTTCTTCTTCCTCACTACCATCTTTCTTTTCTCCATCTCATCATGGGAAGTTGAGGCTCATCCAGTATCTACTTTGATCAACAAAAAGCTTTACAACAACTTGTTTCTACACAAGGACGACACAGCATGCCCTGCAAATGATTTCTACACTTATCGCTCCTTCATCGAGGCAACCAAATACTTTCCGAGATTCGGTACCACTGGAAGTCTAGCCACTCGAAAGCGTGAAATTGCAGCATTTCTTGCTCAGATCTCTCACGAGACCACAGGCGGGTGGGCTACAGCGCCCGACGGACCATACTCTTGGGGTTTGTGCTTTAAAGAGGAAGTCAATCCGGGAAGCAACTATTGCGACAGCACCAACAAGGAGTGGCCATGCTATCCTGGAAAATCTTACAAAGGAAGAGGACCAATTCAACTATCTTGGAACTATAATTATGGTCAGGCAGGCAAGGCATTAGGGTTTGATGGACTAAAAAATCCAGAAGTAGTAGCAAACAATTCCTTAATAGCTTTCAAAACAGCTATATGGTTCTGGATGACTGAGCAAAAGCCGAAGCCCTCTTGTCACGATGTCATGGTGGGTCGATATGTGCTCACGCAAGCTGACATGGCTGCTAATAGGACTGTCGGATTCGGGATGGTGACGAACATCATCAACGGTGGACTTGAGTGTGGAATAGGCAGCGATGCACGAGTGAATGATCGAATTGGTTATTTCAAAAGATATGCTAGTCTGTTTGGTGTTCAAACAGGACCAAGTTTAGACTGTGAAAATCAGAAGTCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_19

Protein Analysis

276

Amino Acids

30.8

Weight (kDa)

8.39

Isoelectric Point (pI)

28.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 38 - 270 1.2e-106 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 1 cut(s) 233
AciI CCGC 2 cut(s) 8, 309
AcoI YGGCCR 1 cut(s) 410
AcuI CTGAAG 1 cut(s) 9
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 2 cut(s) 230, 309
AgsI TTSAA 4 cut(s) 455, 556, 760, 788
AjnI CCWGG 1 cut(s) 421
AluBI AGCT 4 cut(s) 127, 551, 563, 644
AluI AGCT 4 cut(s) 127, 551, 563, 644
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 1 cut(s) 293
AoxI GGCC 1 cut(s) 410
ApeKI GCWGC 4 cut(s) 5, 272, 653, 723
Asp718I GGTACC 1 cut(s) 233
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 3 cut(s) 332, 446, 794
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 1 cut(s) 694
AvaII GGWCC 3 cut(s) 332, 446, 794
BalI TGGCCA 1 cut(s) 412
BanI GGYRCC 1 cut(s) 233
BauI CACGAG 2 cut(s) 296, 732
Bbv12I GWGCWC 1 cut(s) 636
BbvI GCAGC 3 cut(s) 284, 640, 735
BccI CCATC 4 cut(s) 65, 80, 500, 673
BciT130I CCWGG 1 cut(s) 423
BclI TGATCA 1 cut(s) 114
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 2 cut(s) 248, 771
BfmI CTRYAG 1 cut(s) 318
BfoI RGCGCY 1 cut(s) 326
BglII AGATCT 1 cut(s) 288
BisI GCNGC 4 cut(s) 6, 273, 654, 724
BlsI GCNGC 4 cut(s) 7, 274, 655, 725
Bme1390I CCNGG 2 cut(s) 375, 423
Bme18I GGWCC 3 cut(s) 332, 446, 794
BmgT120I GGNCC 3 cut(s) 332, 446, 794
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 2 cut(s) 375, 423
BmsI GCATC 1 cut(s) 718
BpuEI CTTGAG 1 cut(s) 728
BpuMI CCSGG 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 293
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 309
Bse1I ACTGG 2 cut(s) 101, 244
BseBI CCWGG 1 cut(s) 423
BseGI GGATG 3 cut(s) 97, 582, 684
BseLI CCNNNNNNNGG 2 cut(s) 230, 309
BseMII CTCAG 2 cut(s) 299, 573
BseNI ACTGG 2 cut(s) 101, 244
BseXI GCAGC 3 cut(s) 284, 640, 735
BshFI GGCC 1 cut(s) 412
BshNI GGYRCC 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 636
BsiSI CCGG 1 cut(s) 374
BslI CCNNNNNNNGG 2 cut(s) 230, 309
BsmAI GTCTC 1 cut(s) 293
BsnI GGCC 1 cut(s) 412
Bso31I GGTCTC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 636
Bsp143I GATC 3 cut(s) 114, 288, 742
BspACI CCGC 2 cut(s) 8, 309
BspANI GGCC 1 cut(s) 412
BspCNI CTCAG 2 cut(s) 298, 574
BspLI GGNNCC 1 cut(s) 235
BspT107I GGYRCC 1 cut(s) 233
BspTNI GGTCTC 1 cut(s) 293
BsrI ACTGG 2 cut(s) 101, 244
BssMI GATC 3 cut(s) 114, 288, 742
BssSI CACGAG 2 cut(s) 296, 732
Bst2BI CACGAG 2 cut(s) 296, 732
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 3 cut(s) 667, 701, 809
Bst6I CTCTTC 1 cut(s) 436
BstAPI GCANNNNNTGC 3 cut(s) 170, 281, 387
BstC8I GCNNGC 3 cut(s) 166, 487, 642
BstDEI CTNAG 2 cut(s) 285, 582
BstF5I GGATG 3 cut(s) 97, 582, 684
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 3 cut(s) 117, 291, 745
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 3 cut(s) 114, 288, 742
BstMWI GCNNNNNNNGC 5 cut(s) 170, 281, 387, 491, 650
BstNI CCWGG 1 cut(s) 423
BstNSI RCATGY 1 cut(s) 168
BstSCI CCNGG 2 cut(s) 373, 421
BstSFI CTRYAG 1 cut(s) 318
BstV1I GCAGC 3 cut(s) 284, 640, 735
BstX2I RGATCY 1 cut(s) 288
BstXI CCANNNNNNTGG 1 cut(s) 342
BstYI RGATCY 1 cut(s) 288
BsuRI GGCC 1 cut(s) 412
BtgZI GCGATG 1 cut(s) 741
BtsCI GGATG 3 cut(s) 97, 582, 684
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 3 cut(s) 166, 487, 642
CfoI GCGC 1 cut(s) 325
Cfr13I GGNCC 3 cut(s) 332, 446, 794
Csp6I GTAC 1 cut(s) 234
CviAII CATG 5 cut(s) 81, 165, 414, 616, 649
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 2 cut(s) 285, 582
DpnI GATC 3 cut(s) 116, 290, 744
DpnII GATC 3 cut(s) 114, 288, 742
DraI TTTAAA 1 cut(s) 358
EaeI YGGCCR 1 cut(s) 410
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 3 cut(s) 332, 446, 794
Eco57I CTGAAG 1 cut(s) 9
EcoRII CCWGG 1 cut(s) 421
FaeI CATG 5 cut(s) 84, 168, 417, 619, 652
FatI CATG 5 cut(s) 80, 164, 413, 615, 648
FauI CCCGC 1 cut(s) 302
FbaI TGATCA 1 cut(s) 114
Fnu4HI GCNGC 4 cut(s) 6, 273, 654, 724
FokI GGATG 3 cut(s) 84, 589, 691
Fsp4HI GCNGC 4 cut(s) 6, 273, 654, 724
FspBI CTAG 2 cut(s) 248, 771
GlaI GCGC 1 cut(s) 324
GluI GCNGC 4 cut(s) 6, 273, 654, 724
HaeII RGCGCY 1 cut(s) 326
HaeIII GGCC 1 cut(s) 412
HapII CCGG 1 cut(s) 374
HhaI GCGC 1 cut(s) 325
Hin1II CATG 5 cut(s) 84, 168, 417, 619, 652
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 2 cut(s) 228, 672
HpaII CCGG 1 cut(s) 374
HphI GGTGA 1 cut(s) 694
Hpy166II GTNNAC 1 cut(s) 704
Hpy188I TCNGA 4 cut(s) 225, 288, 671, 819
Hpy188III TCNNGA 5 cut(s) 296, 521, 574, 608, 676
Hpy8I GTNNAC 1 cut(s) 704
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 2 cut(s) 40, 208
HpyCH4III ACNGT 3 cut(s) 667, 701, 809
HpyCH4V TGCA 3 cut(s) 173, 272, 731
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 281, 387, 491, 650
HpyF3I CTNAG 2 cut(s) 285, 582
Hsp92II CATG 5 cut(s) 84, 168, 417, 619, 652
HspAI GCGC 1 cut(s) 323
KpnI GGTACC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 114
Kzo9I GATC 3 cut(s) 114, 288, 742
LmnI GCTCC 1 cut(s) 200
Lsp1109I GCAGC 3 cut(s) 284, 640, 735
LweI GCATC 1 cut(s) 718
MaeI CTAG 2 cut(s) 248, 771
MaeIII GTNAC 2 cut(s) 605, 682
MalI GATC 3 cut(s) 116, 290, 744
MboI GATC 3 cut(s) 114, 288, 742
MboII GAAGA 7 cut(s) 15, 25, 28, 31, 34, 37, 453
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 1 cut(s) 636
MlsI TGGCCA 1 cut(s) 412
MluCI AATT 5 cut(s) 267, 450, 472, 538, 747
MluNI TGGCCA 1 cut(s) 412
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 6 cut(s) 59, 85, 199, 355, 437, 610
Mox20I TGGCCA 1 cut(s) 412
MscI TGGCCA 1 cut(s) 412
MseI TTAA 3 cut(s) 357, 545, 829
Msp20I TGGCCA 1 cut(s) 412
MspI CCGG 1 cut(s) 374
MspR9I CCNGG 2 cut(s) 375, 423
MvaI CCWGG 1 cut(s) 423
MwoI GCNNNNNNNGC 5 cut(s) 170, 281, 387, 491, 650
NciI CCSGG 1 cut(s) 375
NdeII GATC 3 cut(s) 114, 288, 742
NlaIII CATG 5 cut(s) 84, 168, 417, 619, 652
NlaIV GGNNCC 1 cut(s) 235
NmuCI GTSAC 2 cut(s) 605, 682
NspI RCATGY 1 cut(s) 168
PaeI GCATGC 1 cut(s) 168
PfeI GAWTC 2 cut(s) 228, 672
PfoI TCCNGGA 2 cut(s) 373, 421
PkrI GCNGC 4 cut(s) 7, 274, 655, 725
Psp6I CCWGG 1 cut(s) 421
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 3 cut(s) 332, 446, 794
PsuI RGATCY 1 cut(s) 288
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SaqAI TTAA 3 cut(s) 357, 545, 829
SatI GCNGC 4 cut(s) 6, 273, 654, 724
Sau3AI GATC 3 cut(s) 114, 288, 742
Sau96I GGNCC 3 cut(s) 332, 446, 794
ScrFI CCNGG 2 cut(s) 375, 423
SduI GDGCHC 1 cut(s) 636
SetI ASST 4 cut(s) 129, 553, 565, 646
SfaNI GCATC 1 cut(s) 718
SfcI CTRYAG 1 cut(s) 318
SinI GGWCC 3 cut(s) 332, 446, 794
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
SphI GCATGC 1 cut(s) 168
Sse9I AATT 5 cut(s) 267, 450, 472, 538, 747
SsiI CCGC 2 cut(s) 8, 309
SspMI CTAG 2 cut(s) 248, 771
StyD4I CCNGG 2 cut(s) 373, 421
TaaI ACNGT 3 cut(s) 667, 701, 809
TaqI TCGA 4 cut(s) 204, 256, 625, 745
TasI AATT 5 cut(s) 267, 450, 472, 538, 747
TfiI GAWTC 2 cut(s) 228, 672
Tru1I TTAA 3 cut(s) 357, 545, 829
Tru9I TTAA 3 cut(s) 357, 545, 829
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 2 cut(s) 605, 682
TseI GCWGC 4 cut(s) 5, 272, 653, 723
Tsp45I GTSAC 2 cut(s) 605, 682
TspDTI ATGAA 1 cut(s) 190
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 1 cut(s) 244
VpaK11BI GGWCC 3 cut(s) 332, 446, 794
XceI RCATGY 1 cut(s) 168
XspI CTAG 2 cut(s) 248, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.