Rroxscaffold_6G00396530

chitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005224
Physical Location & Seq
Forward (+)
18060698 .. 18064262
3565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00396530.1

Sequence Viewer

Length: 753 bp
ATGGCTGCTGTCTCGCTGTCATCTGCAGTCTTCCTCTTCTCCCTCACTACCATCTTTCTTTTCTCCATCTCATCATGGGAAGCTGAGGCTTATCCAGACGACAAGGCATGCCCTGCAAATGACTTCTATACCTACCGCTCCTTCATCAAGGCAACCAAATTCTTCCCGGAATTCGGCACCACTGGAAGTCTAGCCACCCGAAAGCGCGAGATTGCAGCATTTCTTGCACAGATCTCTCACGAGACCACGGGAGGGTGGGCTACTGCCCCCGACGGACCATATTCGTGGGGTTTGTGCTATAAAGAGGAAATCAGTCCGACCAGCAATTACTGTGATAATACCAACAAAGAGTGGCCATGCTATCCTGGCAAATCTTACAAAGGAAGAGGACCAGTTCAATTATCTTGGAACTATAATTATGGCCAGGCAGGCAAGGCATTAGGGTTTGATGGACTAAAGAATCCAGAATTAGTAGCAAACAGTTCCATCATCGCATTCAAAACTGCTTTATGGTTCTGGATGACTGAGCAAAAGCCGAAGCCCTCATGCCACGATGTCATGGTAGGTCGATATGCGCAAACGCAAGATGATGTGGCGGCGAATAGGACCGTCGGGTTCGGGATGGTGACGAACATCATCAACGGTGAACTCGAGTGCGGAATAGGTAGCGATGCACGATTTCAGCACTACAAATGGAACGAGCAATTCAAAATTCACACTGAAGCAATTATTGACGCCACAGCTCCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_19

Protein Analysis

250

Amino Acids

27.87

Weight (kDa)

6.1

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 33 - 228 3.2e-91 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 576
AccB1I GGYRCC 1 cut(s) 176
AccBSI CCGCTC 1 cut(s) 138
AccII CGCG 1 cut(s) 207
AciI CCGC 3 cut(s) 136, 596, 657
AcoI YGGCCR 2 cut(s) 353, 421
AcsI RAATTY 3 cut(s) 158, 170, 711
AcuI CTGAAG 1 cut(s) 741
AcyI GRCGYC 1 cut(s) 735
AfiI CCNNNNNNNGG 2 cut(s) 173, 252
AgsI TTSAA 3 cut(s) 398, 499, 709
AjnI CCWGG 2 cut(s) 364, 423
AluBI AGCT 2 cut(s) 83, 743
AluI AGCT 2 cut(s) 83, 743
Alw26I GTCTC 2 cut(s) 16, 236
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 2 cut(s) 353, 421
ApeKI GCWGC 2 cut(s) 5, 215
ApoI RAATTY 3 cut(s) 158, 170, 711
AspLEI GCGC 2 cut(s) 207, 577
AspS9I GGNCC 3 cut(s) 275, 389, 606
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 2 cut(s) 637, 656
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 3 cut(s) 275, 389, 606
BalI TGGCCA 2 cut(s) 355, 423
BanI GGYRCC 1 cut(s) 176
BauI CACGAG 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 227
BccI CCATC 5 cut(s) 59, 74, 443, 494, 616
BciT130I CCWGG 2 cut(s) 366, 425
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 2 cut(s) 16, 236
BfaI CTAG 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 24
BglI GCCNNNNNGGC 1 cut(s) 429
BglII AGATCT 1 cut(s) 231
BisI GCNGC 3 cut(s) 6, 216, 597
BlsI GCNGC 3 cut(s) 7, 217, 598
Bme1390I CCNGG 3 cut(s) 167, 366, 425
Bme18I GGWCC 3 cut(s) 275, 389, 606
BmeT110I CYCGRG 1 cut(s) 650
BmgT120I GGNCC 3 cut(s) 275, 389, 606
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 3 cut(s) 167, 366, 425
BmsI GCATC 1 cut(s) 661
BpiI GAAGAC 1 cut(s) 22
Bpu10I CCTNAGC 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 167
BsaHI GRCGYC 1 cut(s) 735
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 246, 746
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 252
Bse1I ACTGG 2 cut(s) 187, 392
BseBI CCWGG 2 cut(s) 366, 425
BseDI CCNNGG 2 cut(s) 246, 746
BseGI GGATG 2 cut(s) 525, 627
BseLI CCNNNNNNNGG 2 cut(s) 173, 252
BseMII CTCAG 2 cut(s) 75, 516
BseNI ACTGG 2 cut(s) 187, 392
BseXI GCAGC 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 207
BshFI GGCC 2 cut(s) 355, 423
BshNI GGYRCC 1 cut(s) 176
BsiHKCI CYCGRG 1 cut(s) 650
BsiSI CCGG 1 cut(s) 167
BslI CCNNNNNNNGG 2 cut(s) 173, 252
BsmAI GTCTC 2 cut(s) 16, 236
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 2 cut(s) 355, 423
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 650
Bsp143I GATC 1 cut(s) 231
BspACI CCGC 3 cut(s) 136, 596, 657
BspANI GGCC 2 cut(s) 355, 423
BspCNI CTCAG 2 cut(s) 76, 517
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 1 cut(s) 178
BspMAI CTGCAG 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 236
BsrBI CCGCTC 1 cut(s) 138
BsrI ACTGG 2 cut(s) 187, 392
BssECI CCNNGG 2 cut(s) 246, 746
BssMI GATC 1 cut(s) 231
BssNI GRCGYC 1 cut(s) 735
BssSI CACGAG 1 cut(s) 239
BssT1I CCWWGG 1 cut(s) 746
Bst2BI CACGAG 1 cut(s) 239
Bst2UI CCWGG 2 cut(s) 366, 425
Bst4CI ACNGT 4 cut(s) 332, 482, 610, 644
Bst6I CTCTTC 2 cut(s) 41, 379
BstACI GRCGYC 1 cut(s) 735
BstAPI GCANNNNNTGC 2 cut(s) 113, 224
BstC8I GCNNGC 2 cut(s) 109, 430
BstDEI CTNAG 2 cut(s) 84, 525
BstDSI CCRYGG 1 cut(s) 246
BstF5I GGATG 2 cut(s) 525, 627
BstFNI CGCG 1 cut(s) 207
BstHHI GCGC 2 cut(s) 207, 577
BstKTI GATC 1 cut(s) 234
BstMAI GTCTC 2 cut(s) 16, 236
BstMBI GATC 1 cut(s) 231
BstMWI GCNNNNNNNGC 5 cut(s) 113, 224, 366, 429, 434
BstNI CCWGG 2 cut(s) 366, 425
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 3 cut(s) 165, 364, 423
BstSFI CTRYAG 1 cut(s) 24
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 1 cut(s) 227
BstV2I GAAGAC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 231
BstXI CCANNNNNNTGG 1 cut(s) 285
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 2 cut(s) 355, 423
BtgI CCRYGG 1 cut(s) 246
BtgZI GCGATG 2 cut(s) 475, 684
BtsCI GGATG 2 cut(s) 525, 627
BtsIMutI CAGTG 2 cut(s) 180, 717
Cac8I GCNNGC 2 cut(s) 109, 430
CfoI GCGC 2 cut(s) 207, 577
Cfr13I GGNCC 3 cut(s) 275, 389, 606
CseI GACGC 1 cut(s) 743
CviAII CATG 5 cut(s) 75, 108, 357, 546, 559
DdeI CTNAG 2 cut(s) 84, 525
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
EaeI YGGCCR 2 cut(s) 353, 421
Eam1104I CTCTTC 2 cut(s) 41, 379
EarI CTCTTC 2 cut(s) 41, 379
Eco130I CCWWGG 1 cut(s) 746
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 3 cut(s) 275, 389, 606
Eco57I CTGAAG 1 cut(s) 741
Eco88I CYCGRG 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 2 cut(s) 364, 423
EcoT14I CCWWGG 1 cut(s) 746
ErhI CCWWGG 1 cut(s) 746
FaeI CATG 5 cut(s) 78, 111, 360, 549, 562
FatI CATG 5 cut(s) 74, 107, 356, 545, 558
Fnu4HI GCNGC 3 cut(s) 6, 216, 597
FokI GGATG 2 cut(s) 532, 634
Fsp4HI GCNGC 3 cut(s) 6, 216, 597
FspBI CTAG 1 cut(s) 191
FspI TGCGCA 1 cut(s) 576
GlaI GCGC 2 cut(s) 206, 576
GluI GCNGC 3 cut(s) 6, 216, 597
HaeIII GGCC 2 cut(s) 355, 423
HapII CCGG 1 cut(s) 167
HgaI GACGC 1 cut(s) 743
HhaI GCGC 2 cut(s) 207, 577
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 5 cut(s) 78, 111, 360, 549, 562
Hin6I GCGC 2 cut(s) 205, 575
HinP1I GCGC 2 cut(s) 205, 575
HinfI GANTC 1 cut(s) 460
HpaII CCGG 1 cut(s) 167
HphI GGTGA 2 cut(s) 637, 656
Hpy166II GTNNAC 1 cut(s) 647
Hpy188I TCNGA 1 cut(s) 318
Hpy188III TCNNGA 5 cut(s) 95, 239, 464, 517, 619
Hpy8I GTNNAC 1 cut(s) 647
Hpy99I CGWCG 2 cut(s) 275, 614
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 4 cut(s) 332, 482, 610, 644
HpyCH4V TGCA 5 cut(s) 26, 116, 215, 227, 674
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 224, 366, 429, 434
HpyF3I CTNAG 2 cut(s) 84, 525
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 5 cut(s) 78, 111, 360, 549, 562
HspAI GCGC 2 cut(s) 205, 575
Kzo9I GATC 1 cut(s) 231
LmnI GCTCC 2 cut(s) 143, 748
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 1 cut(s) 661
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 625
MalI GATC 1 cut(s) 233
MbiI CCGCTC 1 cut(s) 138
MboI GATC 1 cut(s) 231
MboII GAAGA 4 cut(s) 22, 28, 154, 396
MflI RGATCY 1 cut(s) 231
MlsI TGGCCA 2 cut(s) 355, 423
MluCI AATT 9 cut(s) 158, 170, 325, 398, 415, 467, 704, 711, 726
MluNI TGGCCA 2 cut(s) 355, 423
MmeI TCCRAC 1 cut(s) 341
MnlI CCTC 7 cut(s) 44, 53, 79, 245, 298, 380, 553
Mox20I TGGCCA 2 cut(s) 355, 423
MscI TGGCCA 2 cut(s) 355, 423
MslI CAYNNNNRTG 1 cut(s) 283
Msp20I TGGCCA 2 cut(s) 355, 423
MspI CCGG 1 cut(s) 167
MspR9I CCNGG 3 cut(s) 167, 366, 425
Mva1269I GAATGC 1 cut(s) 494
MvaI CCWGG 2 cut(s) 366, 425
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 5 cut(s) 113, 224, 366, 429, 434
NciI CCSGG 1 cut(s) 167
NdeII GATC 1 cut(s) 231
NlaIII CATG 5 cut(s) 78, 111, 360, 549, 562
NlaIV GGNNCC 1 cut(s) 178
NmuCI GTSAC 1 cut(s) 625
NsbI TGCGCA 1 cut(s) 576
NspI RCATGY 1 cut(s) 111
PaeI GCATGC 1 cut(s) 111
PaeR7I CTCGAG 1 cut(s) 650
PcsI WCGNNNNNNNCGW 1 cut(s) 648
PctI GAATGC 1 cut(s) 494
PfeI GAWTC 1 cut(s) 460
PfoI TCCNGGA 1 cut(s) 165
PkrI GCNGC 3 cut(s) 7, 217, 598
Psp6I CCWGG 2 cut(s) 364, 423
PspGI CCWGG 2 cut(s) 364, 423
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 3 cut(s) 275, 389, 606
PspXI VCTCGAGB 1 cut(s) 650
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 1 cut(s) 231
RseI CAYNNNNRTG 1 cut(s) 283
SatI GCNGC 3 cut(s) 6, 216, 597
Sau3AI GATC 1 cut(s) 231
Sau96I GGNCC 3 cut(s) 275, 389, 606
ScrFI CCNGG 3 cut(s) 167, 366, 425
SetI ASST 6 cut(s) 85, 134, 568, 667, 745, 752
SfaNI GCATC 1 cut(s) 661
SfcI CTRYAG 1 cut(s) 24
Sfr274I CTCGAG 1 cut(s) 650
SinI GGWCC 3 cut(s) 275, 389, 606
SlaI CTCGAG 1 cut(s) 650
SmiMI CAYNNNNRTG 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
SphI GCATGC 1 cut(s) 111
Sse9I AATT 9 cut(s) 158, 170, 325, 398, 415, 467, 704, 711, 726
SsiI CCGC 3 cut(s) 136, 596, 657
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 3 cut(s) 165, 364, 423
StyI CCWWGG 1 cut(s) 746
TaaI ACNGT 4 cut(s) 332, 482, 610, 644
TaqI TCGA 2 cut(s) 568, 651
TasI AATT 9 cut(s) 158, 170, 325, 398, 415, 467, 704, 711, 726
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 1 cut(s) 460
TscAI CASTG 2 cut(s) 187, 724
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 2 cut(s) 5, 215
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 1 cut(s) 133
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 2 cut(s) 187, 724
VpaK11BI GGWCC 3 cut(s) 275, 389, 606
XapI RAATTY 3 cut(s) 158, 170, 711
XceI RCATGY 1 cut(s) 111
XhoI CTCGAG 1 cut(s) 650
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.