MD00G1016200.v1.1

chitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005224
Physical Location & Seq
Forward (+)
2341050 .. 2342330
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1016200.v1.1.491

Sequence Viewer

Length: 864 bp
ATGGCACTACCCTCGTTCAACTTGTCCACTTCCTTCTTCCTTGTCTCATTCGCCTTCTTTAGCATTGCCATCAATCTTTTCTCTACTAGTAATGCACCATCCAGAGCTGAGGCTGCTCGCAGCGATTGTCTTCCAATTTCGTCTTTAATCAGCAAAAAGCTTTTCCACACCTTGTTTCTACACAAGGACGACATGGCATGCCCTGCCAAAGACTTCTATACCTACCACTCTTTCATCAAAGCCTCCAAATCCTTCCCCGGATTTGGCACCACTGGAAGTTTAACCACACGCAAGAGTGAGATCGCCGCCTTTCTAGCGCAGATTTCTCACGAGACTACAGGTGGGTGGGCGACTGCCCCTGATGGACCATATGCATGGGGTCTGTGCTTCAAAGAGGAAATCAGTCCCCCTAGCGACTACTGTGACTCCAACAACAAGGAATGGCCTTGTTATCCGGGCAAATCTTACAAAGGAAGAGGCCCTATTCAGCTATCTTGGAACTATAATTATGGACCTGCTGGCAAGGCGTTAGGGTTTGATGGGCTGAAGAATCCTGAAGTAGTGGCAAACAATTCCATGATAGCACTGAAAACGGCTCTATGGTTCTGGATGACTGAGCAAAAGCCAAAGCCCTCTTCTCACGATGTCATGGTTGGACTGTATGTGCCCACAGAAGCTGACGTTGCGGCCAATCGGACTGTTGGATATGGGCTGGTCACTAACATCATCAATGGTAGACTGGAGTGCGGAATACCCAACGATGCACGAGTAAATGATCGGATTGGTTATTTCAGAAGATATGCTGGGTTGCTTAATGTTGATACTGGACCAAATTTAGATTGTGAAAATCAAAAGTCCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_19

Protein Analysis

288

Amino Acids

31.59

Weight (kDa)

8.09

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 49 - 281 2e-107 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 523
AccB1I GGYRCC 1 cut(s) 266
AccI GTMKAC 1 cut(s) 736
AciI CCGC 3 cut(s) 306, 686, 747
AcoI YGGCCR 1 cut(s) 687
AcsI RAATTY 1 cut(s) 832
AcuI CTGAAG 2 cut(s) 566, 576
AfiI CCNNNNNNNGG 1 cut(s) 263
AgsI TTSAA 2 cut(s) 19, 391
AhlI ACTAGT 1 cut(s) 86
AjuI GAANNNNNNNTTGG 2 cut(s) 619, 651
AluBI AGCT 4 cut(s) 107, 160, 490, 677
AluI AGCT 4 cut(s) 107, 160, 490, 677
Alw26I GTCTC 2 cut(s) 49, 326
AlwNI CAGNNNCTG 1 cut(s) 677
AoxI GGCC 3 cut(s) 443, 478, 687
ApeKI GCWGC 2 cut(s) 113, 120
ApoI RAATTY 1 cut(s) 832
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 4 cut(s) 365, 479, 512, 827
AsuC2I CCSGG 2 cut(s) 258, 456
AvaII GGWCC 3 cut(s) 365, 512, 827
BaeGI GKGCMC 1 cut(s) 669
BanI GGYRCC 1 cut(s) 266
BauI CACGAG 2 cut(s) 329, 765
BbsI GAAGAC 1 cut(s) 122
BbvCI CCTCAGC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 100, 132
BccI CCATC 4 cut(s) 77, 106, 356, 533
BceAI ACGGC 1 cut(s) 609
BcgI CGANNNNNNTGC 1 cut(s) 28
BcnI CCSGG 2 cut(s) 258, 456
BcoDI GTCTC 2 cut(s) 49, 326
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 3 cut(s) 87, 314, 411
BfmI CTRYAG 1 cut(s) 336
BfuAI ACCTGC 1 cut(s) 523
BisI GCNGC 4 cut(s) 114, 121, 306, 687
BlsI GCNGC 4 cut(s) 115, 122, 307, 688
Bme1390I CCNGG 2 cut(s) 258, 456
Bme18I GGWCC 3 cut(s) 365, 512, 827
BmgT120I GGNCC 4 cut(s) 365, 479, 512, 827
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 2 cut(s) 258, 456
BmsI GCATC 1 cut(s) 751
BpiI GAAGAC 1 cut(s) 122
BpmI CTGGAG 1 cut(s) 761
Bpu10I CCTNAGC 1 cut(s) 108
BpuMI CCSGG 2 cut(s) 258, 456
BsaJI CCNNGG 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 277, 744, 829
Bse3DI GCAATG 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 2 cut(s) 98, 615
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMI GCAATG 1 cut(s) 63
BseMII CTCAG 2 cut(s) 99, 606
BseNI ACTGG 3 cut(s) 277, 744, 829
BseSI GKGCMC 1 cut(s) 669
BseXI GCAGC 2 cut(s) 100, 132
BseYI CCCAGC 1 cut(s) 803
BshFI GGCC 3 cut(s) 445, 480, 689
BshNI GGYRCC 1 cut(s) 266
BsiSI CCGG 2 cut(s) 258, 455
BslFI GGGAC 1 cut(s) 390
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 2 cut(s) 49, 326
BsmFI GGGAC 1 cut(s) 390
BsnI GGCC 3 cut(s) 445, 480, 689
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 2 cut(s) 300, 775
BspACI CCGC 3 cut(s) 306, 686, 747
BspANI GGCC 3 cut(s) 445, 480, 689
BspCNI CTCAG 2 cut(s) 100, 607
BspLI GGNNCC 1 cut(s) 268
BspMI ACCTGC 1 cut(s) 523
BspT107I GGYRCC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 63
BsrI ACTGG 3 cut(s) 277, 744, 829
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 2 cut(s) 300, 775
BssSI CACGAG 2 cut(s) 329, 765
Bst2BI CACGAG 2 cut(s) 329, 765
Bst4CI ACNGT 3 cut(s) 422, 660, 700
Bst6I CTCTTC 2 cut(s) 469, 640
BstAPI GCANNNNNTGC 1 cut(s) 203
BstC8I GCNNGC 3 cut(s) 118, 199, 520
BstDEI CTNAG 2 cut(s) 108, 615
BstF5I GGATG 2 cut(s) 98, 615
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 2 cut(s) 303, 778
BstMAI GTCTC 2 cut(s) 49, 326
BstMBI GATC 2 cut(s) 300, 775
BstMWI GCNNNNNNNGC 5 cut(s) 113, 203, 314, 524, 683
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 2 cut(s) 256, 454
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 669
BstV1I GCAGC 2 cut(s) 100, 132
BstV2I GAAGAC 1 cut(s) 122
BstXI CCANNNNNNTGG 1 cut(s) 375
BsuRI GGCC 3 cut(s) 445, 480, 689
BtsCI GGATG 2 cut(s) 98, 615
BtsIMutI CAGTG 2 cut(s) 270, 584
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 3 cut(s) 118, 199, 520
CaiI CAGNNNCTG 1 cut(s) 677
CfoI GCGC 1 cut(s) 319
Cfr13I GGNCC 4 cut(s) 365, 479, 512, 827
CviAII CATG 5 cut(s) 193, 198, 375, 577, 649
DdeI CTNAG 2 cut(s) 108, 615
DpnI GATC 2 cut(s) 302, 777
DpnII GATC 2 cut(s) 300, 775
EaeI YGGCCR 1 cut(s) 687
Eam1104I CTCTTC 2 cut(s) 469, 640
EarI CTCTTC 2 cut(s) 469, 640
Eco47I GGWCC 3 cut(s) 365, 512, 827
Eco57I CTGAAG 2 cut(s) 566, 576
EcoO109I RGGNCCY 1 cut(s) 479
EcoT22I ATGCAT 1 cut(s) 376
FaeI CATG 5 cut(s) 196, 201, 378, 580, 652
FaqI GGGAC 1 cut(s) 390
FatI CATG 5 cut(s) 192, 197, 374, 576, 648
FauNDI CATATG 1 cut(s) 370
FblI GTMKAC 1 cut(s) 736
Fnu4HI GCNGC 4 cut(s) 114, 121, 306, 687
FokI GGATG 2 cut(s) 85, 622
Fsp4HI GCNGC 4 cut(s) 114, 121, 306, 687
FspBI CTAG 3 cut(s) 87, 314, 411
GlaI GCGC 1 cut(s) 318
GluI GCNGC 4 cut(s) 114, 121, 306, 687
GsaI CCCAGC 1 cut(s) 807
GsuI CTGGAG 1 cut(s) 761
HaeIII GGCC 3 cut(s) 445, 480, 689
HapII CCGG 2 cut(s) 258, 455
HhaI GCGC 1 cut(s) 319
Hin1II CATG 5 cut(s) 196, 201, 378, 580, 652
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 2 cut(s) 425, 550
HpaII CCGG 2 cut(s) 258, 455
Hpy166II GTNNAC 2 cut(s) 27, 737
Hpy188I TCNGA 3 cut(s) 696, 780, 794
Hpy188III TCNNGA 5 cut(s) 102, 329, 554, 607, 641
Hpy8I GTNNAC 2 cut(s) 27, 737
HpyAV CCTTC 3 cut(s) 43, 64, 262
HpyCH4III ACNGT 3 cut(s) 422, 660, 700
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 3 cut(s) 95, 374, 764
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 203, 314, 524, 683
HpyF3I CTNAG 2 cut(s) 108, 615
HpySE526I ACGT 1 cut(s) 681
Hsp92II CATG 5 cut(s) 196, 201, 378, 580, 652
HspAI GCGC 1 cut(s) 317
Kzo9I GATC 2 cut(s) 300, 775
Lsp1109I GCAGC 2 cut(s) 100, 132
LweI GCATC 1 cut(s) 751
MaeI CTAG 3 cut(s) 87, 314, 411
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 2 cut(s) 422, 715
MalI GATC 2 cut(s) 302, 777
MboI GATC 2 cut(s) 300, 775
MboII GAAGA 6 cut(s) 28, 122, 486, 559, 627, 807
MhlI GDGCHC 1 cut(s) 669
MluCI AATT 4 cut(s) 135, 505, 571, 832
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 3 cut(s) 453, 634, 682
MnlI CCTC 6 cut(s) 22, 103, 253, 388, 470, 643
Mph1103I ATGCAT 1 cut(s) 376
MseI TTAA 4 cut(s) 146, 281, 813, 862
MslI CAYNNNNRTG 1 cut(s) 373
MspI CCGG 2 cut(s) 258, 455
MspR9I CCNGG 2 cut(s) 258, 456
MwoI GCNNNNNNNGC 5 cut(s) 113, 203, 314, 524, 683
NciI CCSGG 2 cut(s) 258, 456
NdeI CATATG 1 cut(s) 370
NdeII GATC 2 cut(s) 300, 775
NlaIII CATG 5 cut(s) 196, 201, 378, 580, 652
NlaIV GGNNCC 1 cut(s) 268
NmuCI GTSAC 2 cut(s) 422, 715
NsiI ATGCAT 1 cut(s) 376
NspI RCATGY 1 cut(s) 201
PaeI GCATGC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 550
PkrI GCNGC 4 cut(s) 115, 122, 307, 688
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PspFI CCCAGC 1 cut(s) 803
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 4 cut(s) 365, 479, 512, 827
PstNI CAGNNNCTG 1 cut(s) 677
RseI CAYNNNNRTG 1 cut(s) 373
SaqAI TTAA 4 cut(s) 146, 281, 813, 862
SatI GCNGC 4 cut(s) 114, 121, 306, 687
Sau3AI GATC 2 cut(s) 300, 775
Sau96I GGNCC 4 cut(s) 365, 479, 512, 827
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 2 cut(s) 258, 456
SduI GDGCHC 1 cut(s) 669
SetI ASST 9 cut(s) 109, 162, 173, 224, 343, 492, 517, 679, 684
SfaNI GCATC 1 cut(s) 751
SfcI CTRYAG 1 cut(s) 336
SinI GGWCC 3 cut(s) 365, 512, 827
SmiMI CAYNNNNRTG 1 cut(s) 373
SpeI ACTAGT 1 cut(s) 86
SphI GCATGC 1 cut(s) 201
Sse9I AATT 4 cut(s) 135, 505, 571, 832
SsiI CCGC 3 cut(s) 306, 686, 747
SspMI CTAG 3 cut(s) 87, 314, 411
StyD4I CCNGG 2 cut(s) 256, 454
TaaI ACNGT 3 cut(s) 422, 660, 700
TaiI ACGT 1 cut(s) 684
TasI AATT 4 cut(s) 135, 505, 571, 832
TauI GCSGC 2 cut(s) 308, 689
TfiI GAWTC 1 cut(s) 550
Tru1I TTAA 4 cut(s) 146, 281, 813, 862
Tru9I TTAA 4 cut(s) 146, 281, 813, 862
TscAI CASTG 2 cut(s) 277, 591
TseFI GTSAC 2 cut(s) 422, 715
TseI GCWGC 2 cut(s) 113, 120
Tsp45I GTSAC 2 cut(s) 422, 715
TspDTI ATGAA 1 cut(s) 223
TspRI CASTG 2 cut(s) 277, 591
VpaK11BI GGWCC 3 cut(s) 365, 512, 827
XapI RAATTY 1 cut(s) 832
XceI RCATGY 1 cut(s) 201
XmiI GTMKAC 1 cut(s) 736
XspI CTAG 3 cut(s) 87, 314, 411
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.