RLG00000002156

chitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005224
Physical Location & Seq
Forward (+)
26474451 .. 26475295
845 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002156

Sequence Viewer

Length: 483 bp
ATGACACAGCATGCATGCCCTGCAAATGATTTCTACATCTATCGCTCCTTCATCGAGGCAACCAAATACTTTCCTAGATTTGGCACCACCGGAAGTCTAGCCACTCGAAAGCGTGAAATTGCAGCATTTCTTGTTCAGATCTCTCACGAGACCGCAGGCGGGTGGGCTACAGCGCCCGACGGACCATACTCTTGGGGTTTGTGCTTTAAAGAGGAAGTCAATCCGGGAAGCAACTACTGCGACAGCACCAACAAGGAGTGGCCATGCTATCCTGGAAAATCTTACAAAAGAAGAGGACCAATTCAACTATCTTGGACTGTCGGATTCGGGATGGTGACGAACATCATCAACGGTGGACTTGAGTGTGGAATAGGCAGCGATGCGCGAGTGAATGATCGAATTGGTTATTTCAAAAGATATGCTAGTCTATTTGGTGTTCAAACAGGACCAAATTTAGACTGTGAAAATCAAAAGTCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_19

Protein Analysis

161

Amino Acids

17.84

Weight (kDa)

8.52

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 3 - 106 1.9e-41 Chitinase class I
Glyco_hydro_19 PF00182 108 - 154 2.5e-17 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AccII CGCG 1 cut(s) 385
AciI CCGC 2 cut(s) 153, 159
AcoI YGGCCR 1 cut(s) 260
AcsI RAATTY 1 cut(s) 451
AfiI CCNNNNNNNGG 2 cut(s) 80, 159
AgsI TTSAA 3 cut(s) 305, 412, 440
AjnI CCWGG 1 cut(s) 271
Alw26I GTCTC 1 cut(s) 143
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 2 cut(s) 122, 375
ApoI RAATTY 1 cut(s) 451
AspLEI GCGC 2 cut(s) 175, 385
AspS9I GGNCC 3 cut(s) 182, 296, 446
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 1 cut(s) 346
AvaII GGWCC 3 cut(s) 182, 296, 446
BalI TGGCCA 1 cut(s) 262
BanI GGYRCC 1 cut(s) 83
BauI CACGAG 1 cut(s) 146
BbvI GCAGC 2 cut(s) 134, 387
BccI CCATC 1 cut(s) 325
BciT130I CCWGG 1 cut(s) 273
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 3 cut(s) 75, 98, 423
BfmI CTRYAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 176
BglII AGATCT 1 cut(s) 138
BisI GCNGC 2 cut(s) 123, 376
BlsI GCNGC 2 cut(s) 124, 377
Bme1390I CCNGG 2 cut(s) 225, 273
Bme18I GGWCC 3 cut(s) 182, 296, 446
BmgT120I GGNCC 3 cut(s) 182, 296, 446
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 2 cut(s) 225, 273
BmsI GCATC 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 143
BsaWI WCCGGW 1 cut(s) 89
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 159
BseBI CCWGG 1 cut(s) 273
BseGI GGATG 1 cut(s) 336
BseLI CCNNNNNNNGG 2 cut(s) 80, 159
BseXI GCAGC 2 cut(s) 134, 387
Bsh1236I CGCG 1 cut(s) 385
BshFI GGCC 1 cut(s) 262
BshNI GGYRCC 1 cut(s) 83
BsiSI CCGG 2 cut(s) 90, 224
BslI CCNNNNNNNGG 2 cut(s) 80, 159
BsmAI GTCTC 1 cut(s) 143
BsnI GGCC 1 cut(s) 262
Bso31I GGTCTC 1 cut(s) 143
Bsp143I GATC 2 cut(s) 138, 394
BspACI CCGC 2 cut(s) 153, 159
BspANI GGCC 1 cut(s) 262
BspFNI CGCG 1 cut(s) 385
BspLI GGNNCC 1 cut(s) 85
BspT107I GGYRCC 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 143
BssMI GATC 2 cut(s) 138, 394
BssSI CACGAG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 273
Bst4CI ACNGT 3 cut(s) 319, 353, 461
Bst6I CTCTTC 1 cut(s) 286
BstAPI GCANNNNNTGC 2 cut(s) 20, 237
BstC8I GCNNGC 3 cut(s) 12, 16, 157
BstF5I GGATG 1 cut(s) 336
BstFNI CGCG 1 cut(s) 385
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 2 cut(s) 175, 385
BstKTI GATC 2 cut(s) 141, 397
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 2 cut(s) 138, 394
BstMWI GCNNNNNNNGC 2 cut(s) 20, 237
BstNI CCWGG 1 cut(s) 273
BstNSI RCATGY 2 cut(s) 14, 18
BstSCI CCNGG 2 cut(s) 223, 271
BstSFI CTRYAG 1 cut(s) 168
BstUI CGCG 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 134, 387
BstX2I RGATCY 1 cut(s) 138
BstXI CCANNNNNNTGG 1 cut(s) 192
BstYI RGATCY 1 cut(s) 138
BsuRI GGCC 1 cut(s) 262
BtgZI GCGATG 1 cut(s) 393
BtsCI GGATG 1 cut(s) 336
Cac8I GCNNGC 3 cut(s) 12, 16, 157
CfoI GCGC 2 cut(s) 175, 385
Cfr13I GGNCC 3 cut(s) 182, 296, 446
CviAII CATG 3 cut(s) 11, 15, 264
CviJI RGCY 3 cut(s) 101, 167, 262
CviKI_1 RGCY 3 cut(s) 101, 167, 262
DpnI GATC 2 cut(s) 140, 396
DpnII GATC 2 cut(s) 138, 394
DraI TTTAAA 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
Eco31I GGTCTC 1 cut(s) 143
Eco47I GGWCC 3 cut(s) 182, 296, 446
EcoRII CCWGG 1 cut(s) 271
EcoT22I ATGCAT 1 cut(s) 16
FaeI CATG 3 cut(s) 14, 18, 267
FaiI YATR 5 cut(s) 12, 16, 187, 265, 420
FatI CATG 3 cut(s) 10, 14, 263
FauI CCCGC 1 cut(s) 152
Fnu4HI GCNGC 2 cut(s) 123, 376
FokI GGATG 1 cut(s) 343
Fsp4HI GCNGC 2 cut(s) 123, 376
FspBI CTAG 3 cut(s) 75, 98, 423
GlaI GCGC 2 cut(s) 174, 384
GluI GCNGC 2 cut(s) 123, 376
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 1 cut(s) 262
HapII CCGG 2 cut(s) 90, 224
HhaI GCGC 2 cut(s) 175, 385
Hin1II CATG 3 cut(s) 14, 18, 267
Hin6I GCGC 2 cut(s) 173, 383
HinP1I GCGC 2 cut(s) 173, 383
HinfI GANTC 1 cut(s) 324
HpaII CCGG 2 cut(s) 90, 224
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 1 cut(s) 356
Hpy188I TCNGA 2 cut(s) 138, 323
Hpy188III TCNNGA 2 cut(s) 146, 328
Hpy8I GTNNAC 1 cut(s) 356
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 3 cut(s) 319, 353, 461
HpyCH4V TGCA 3 cut(s) 14, 23, 122
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 237
Hsp92II CATG 3 cut(s) 14, 18, 267
HspAI GCGC 2 cut(s) 173, 383
Kzo9I GATC 2 cut(s) 138, 394
LmnI GCTCC 1 cut(s) 50
LpnPI CCDG 7 cut(s) 33, 103, 141, 237, 258, 285, 429
Lsp1109I GCAGC 2 cut(s) 134, 387
LweI GCATC 1 cut(s) 370
MaeI CTAG 3 cut(s) 75, 98, 423
MaeIII GTNAC 1 cut(s) 334
MalI GATC 2 cut(s) 140, 396
MboI GATC 2 cut(s) 138, 394
MboII GAAGA 1 cut(s) 303
MflI RGATCY 1 cut(s) 138
MlsI TGGCCA 1 cut(s) 262
MluCI AATT 4 cut(s) 117, 300, 399, 451
MluNI TGGCCA 1 cut(s) 262
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 3 cut(s) 49, 205, 287
Mox20I TGGCCA 1 cut(s) 262
Mph1103I ATGCAT 1 cut(s) 16
MscI TGGCCA 1 cut(s) 262
MseI TTAA 2 cut(s) 207, 481
Msp20I TGGCCA 1 cut(s) 262
MspI CCGG 2 cut(s) 90, 224
MspR9I CCNGG 2 cut(s) 225, 273
MvaI CCWGG 1 cut(s) 273
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 2 cut(s) 20, 237
NciI CCSGG 1 cut(s) 225
NdeII GATC 2 cut(s) 138, 394
NlaIII CATG 3 cut(s) 14, 18, 267
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 334
NsiI ATGCAT 1 cut(s) 16
NspI RCATGY 2 cut(s) 14, 18
PaeI GCATGC 2 cut(s) 14, 18
PfeI GAWTC 1 cut(s) 324
PfoI TCCNGGA 2 cut(s) 223, 271
PkrI GCNGC 2 cut(s) 124, 377
Psp6I CCWGG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 271
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 3 cut(s) 182, 296, 446
PsuI RGATCY 1 cut(s) 138
SaqAI TTAA 2 cut(s) 207, 481
SatI GCNGC 2 cut(s) 123, 376
Sau3AI GATC 2 cut(s) 138, 394
Sau96I GGNCC 3 cut(s) 182, 296, 446
ScrFI CCNGG 2 cut(s) 225, 273
SfaNI GCATC 1 cut(s) 370
SfcI CTRYAG 1 cut(s) 168
SinI GGWCC 3 cut(s) 182, 296, 446
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SphI GCATGC 2 cut(s) 14, 18
Sse9I AATT 4 cut(s) 117, 300, 399, 451
SsiI CCGC 2 cut(s) 153, 159
SspMI CTAG 3 cut(s) 75, 98, 423
StyD4I CCNGG 2 cut(s) 223, 271
TaaI ACNGT 3 cut(s) 319, 353, 461
TaqI TCGA 3 cut(s) 54, 106, 397
TasI AATT 4 cut(s) 117, 300, 399, 451
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 2 cut(s) 207, 481
Tru9I TTAA 2 cut(s) 207, 481
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 2 cut(s) 122, 375
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 1 cut(s) 40
TspGWI ACGGA 1 cut(s) 195
VpaK11BI GGWCC 3 cut(s) 182, 296, 446
XapI RAATTY 1 cut(s) 451
XceI RCATGY 2 cut(s) 14, 18
XspI CTAG 3 cut(s) 75, 98, 423
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.