Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0005487

Physical Location & Seq

Genomic Coordinates Forward (+)

20609907 .. 20615300

Gene Structure 5394 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g29680.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1281 bp

ATGATCTTTCTCAGAAAACTAACCGGAGCCTCTTCAGCCTCCACTTCACCACCACACATGGCTCCCTACACCTCTCTCCTCCGCCCTCAACCTCACCCCTTCTCTCTCCCCAACCCTAACCTTAACCCTAACCCTAACCTCGTCACCAGGCACACCTTACTCCCTCTCCGCACTGTCAAGCTCAAGACCTCTCTCCCTCACTCCTCCACCGACCCCGACACCAATGGAACAGCGTCGTCAGCTCCGCCTCCACTTCCTCCTCCGGCAACGGCGACGAAGAAGTCGTTTGCGGTTGCGACGGGGGAGTTGTTCCTGGGCCTGGCGTCGAGGATCATCAAGCGGCGGAATGGAGCCCCGGAAAGTGAGACGGCGGCGGCCTCCGTTGCTATGTTTGAGAGCTCCGGGAAGTTCTCCGATGAGAGAATCGGGAAGGTGATGGAGGATGAGATTCAGCCGGGGGTGGTTTGGGAGCAGCGCGTGAAGGATGTGGAGGCGGAGAAAGAGAGGCGCGTGATTACCAGTCCCGGCTTTAGTTTTTCTGCGGCGGGGCTTCTTTTCCCTTATCATTTAGGTGCTGCTCAGTTCTTGATTGAGAATGGCTATATCAAGGAAACCACTCCATTAGCTGGTTCATCAGCAGGTGCCATTGTCTGTGCTGTGATTGCTTCCGGAGCAAGCATGGAAGAGGCCTTACAAGCTACTAAAATTCTGGCTGAAGATTGCCGGAATAGAGGGACAGCTTTCCGACTTGGGGCTGTTCTTCGAGATGTTCTCGAAAAGTTTTTGCCCGAGGATGCTCATATTCGGTCTAATGGGAGGGTCAGAGTTGCTGTAACACAGCTCCTATGGAGACCAAGAGGTTTATTGGTGGATCAGTTTGACTCCAAGGAAGATCTCATTAATGCAGTTTTCACTTCTTCCTTTATTCCAGGATATCTTGCACCAAGACCAGCAACAATGTTCCGGAATCGTCTCTGTATTGACGGGGGATTGACTTTATTTATGCCACCAACATCTGCGGCAAAGACGATTCGTGTTTGTGCTTTCCCAGCTAGTCGATTAGGTTTACAAGGAATTGGGATCAGTCCAGACTGTAATCCTGATGATAGGGCAAGCCCTCGAGAGCTTTTTAATTGGGCACTTGAGCCAGCAGAAGATGATATGCTTGACCGGCTGTTTTCGCTTGGATATAAAGATGCGGCTGTATGGGCCAAGTCCAACCCAGTTGAACAAATAGTCGAAGATGATAGTTCCTTGATTGGTAGTGGCTTAGCTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004806 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005811 GO:0005829 GO:0005886 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006996 GO:0008150 GO:0008152 GO:0009056 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010876 GO:0010883 GO:0010888 GO:0010889 GO:0010891 GO:0010896 GO:0010898 GO:0016020 GO:0016042 GO:0016043 GO:0016298 GO:0016787 GO:0016788 GO:0019216 GO:0019222 GO:0019433 GO:0019915 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031974 GO:0031981 GO:0032879 GO:0033036 GO:0034389 GO:0042592 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045834 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0048518 GO:0048519 GO:0048522 GO:0048878 GO:0050789 GO:0050794 GO:0050994 GO:0050996 GO:0051179 GO:0051235 GO:0052689 GO:0055088 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090207 GO:0090208 GO:1901575 GO:1905952 GO:1905953

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Patatin

Protein Analysis

427

Amino Acids

45.97

Weight (kDa)

7.02

Isoelectric Point (pI)

41.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Patatin	PF01734	178 - 311	2.1e-13	Patatin-like phospholipase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005487)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33270 AT1G33270
fragaria_vesca	FvH4_3g12743 FvH4_3g29702 FvH4_4g06881 FvH4_4g16550 FvH4_5g19441 FvH4_5g29680 FvH4_5g29680 FvH4_5g29680 FvH4_5g29680 FvH4_6g34462 FvH4_6g34463
malus_domestica	MD15G1421100.v1.1
prunus_persica	Prupe.1G566300_v2.0.a1 Prupe.1G566300_v2.0.a1
pyrus_communis	pycom15g37340
rosa_chinensis	RchiOBHm_Chr7g0223001
rosa_laevigata	RLG00000002004
rosa_multiflora	Rmu_sc0006373.1_g000001 Rmu_sc0006514.1_g000023
rosa_roxburghii	Rroxscaffold_3G00236360
rosa_rugosa	Rorug07G0212600 Rorug07G0212700
rosa_samantha	Rh7AG357800 Rh7BG346000 Rh7CG375900 Rh7DG352700
rosa_wichuraiana	Rw7G030430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	629
AasI	GACNNNNNNGTC	1 cut(s)	280
Acc36I	ACCTGC	1 cut(s)	629
AccB1I	GGYRCC	1 cut(s)	641
AccB7I	CCANNNNNTGG	1 cut(s)	626
AccII	CGCG	2 cut(s)	477, 510
AccIII	TCCGGA	2 cut(s)	668, 963
AclWI	GGATC	3 cut(s)	338, 879, 1088
AcsI	RAATTY	1 cut(s)	705
AcuI	CTGAAG	2 cut(s)	18, 735
AcyI	GRCGYC	1 cut(s)	323
AfiI	CCNNNNNNNGG	3 cut(s)	319, 626, 751
AgsI	TTSAA	1 cut(s)	1229
AjnI	CCWGG	4 cut(s)	146, 312, 318, 928
Alw21I	GWGCWC	1 cut(s)	401
Alw26I	GTCTC	3 cut(s)	359, 844, 977
AlwI	GGATC	3 cut(s)	338, 879, 1088
Ama87I	CYCGRG	2 cut(s)	788, 1119
Aor13HI	TCCGGA	2 cut(s)	668, 963
AoxI	GGCC	4 cut(s)	316, 375, 687, 1209
ApeKI	GCWGC	2 cut(s)	472, 575
ApoI	RAATTY	1 cut(s)	705
AseI	ATTAAT	1 cut(s)	900
AspLEI	GCGC	2 cut(s)	477, 510
AspS9I	GGNCC	2 cut(s)	316, 1209
AsuC2I	CCSGG	4 cut(s)	356, 403, 456, 525
AsuHPI	GGTGA	4 cut(s)	39, 86, 136, 445
AvaI	CYCGRG	2 cut(s)	788, 1119
BaeGI	GKGCMC	1 cut(s)	1141
BanI	GGYRCC	1 cut(s)	641
BanII	GRGCYC	2 cut(s)	355, 401
BarI	GAAGNNNNNNTAC	2 cut(s)	675, 707
Bbv12I	GWGCWC	1 cut(s)	401
BbvI	GCAGC	2 cut(s)	484, 562
BccI	CCATC	1 cut(s)	430
BceAI	ACGGC	2 cut(s)	285, 384
BciT130I	CCWGG	4 cut(s)	148, 314, 320, 930
BcnI	CCSGG	4 cut(s)	356, 403, 456, 525
BcoDI	GTCTC	3 cut(s)	359, 844, 977
BfaI	CTAG	1 cut(s)	1053
BfuAI	ACCTGC	1 cut(s)	629
BglII	AGATCT	1 cut(s)	892
BisI	GCNGC	8 cut(s)	341, 372, 375, 473, 543, 576, 1020, 1200
BlpI	GCTNAGC	1 cut(s)	1270
BlsI	GCNGC	8 cut(s)	342, 373, 376, 474, 544, 577, 1021, 1201
Bme1390I	CCNGG	8 cut(s)	148, 314, 320, 356, 403, 456, 525, 930
BmeT110I	CYCGRG	2 cut(s)	788, 1119
BmgT120I	GGNCC	2 cut(s)	316, 1209
BmiI	GGNNCC	4 cut(s)	28, 63, 352, 643
BmrFI	CCNGG	8 cut(s)	148, 314, 320, 356, 403, 456, 525, 930
BmrI	ACTGGG	1 cut(s)	1217
BmsI	GCATC	2 cut(s)	784, 1186
BmuI	ACTGGG	1 cut(s)	1217
BplI	GAGNNNNNCTC	2 cut(s)	756, 788
Bpu1102I	GCTNAGC	1 cut(s)	1270
BpuEI	CTTGAG	2 cut(s)	167, 1163
BpuMI	CCSGG	4 cut(s)	356, 403, 456, 525
BsaHI	GRCGYC	1 cut(s)	323
BsaI	GGTCTC	1 cut(s)	844
BsaJI	CCNNGG	5 cut(s)	313, 354, 455, 789, 885
BsaWI	WCCGGW	3 cut(s)	23, 668, 963
BsaXI	ACNNNNNCTCC	2 cut(s)	150, 180
Bsc4I	CCNNNNNNNGG	3 cut(s)	319, 626, 751
Bse118I	RCCGGY	1 cut(s)	1170
Bse1I	ACTGG	2 cut(s)	519, 1223
BseAI	TCCGGA	2 cut(s)	668, 963
BseBI	CCWGG	4 cut(s)	148, 314, 320, 930
BseDI	CCNNGG	5 cut(s)	313, 354, 455, 789, 885
BseGI	GGATG	3 cut(s)	448, 490, 799
BseLI	CCNNNNNNNGG	3 cut(s)	319, 626, 751
BseMII	CTCAG	2 cut(s)	25, 593
BseNI	ACTGG	2 cut(s)	519, 1223
BseRI	GAGGAG	3 cut(s)	68, 193, 249
BseSI	GKGCMC	1 cut(s)	1141
BseXI	GCAGC	2 cut(s)	484, 562
BseYI	CCCAGC	1 cut(s)	1048
Bsh1236I	CGCG	2 cut(s)	477, 510
BshFI	GGCC	4 cut(s)	318, 377, 689, 1211
BshNI	GGYRCC	1 cut(s)	641
BsiHKAI	GWGCWC	1 cut(s)	401
BsiHKCI	CYCGRG	2 cut(s)	788, 1119
BslFI	GGGAC	2 cut(s)	507, 748
BslI	CCNNNNNNNGG	3 cut(s)	319, 626, 751
BsmAI	GTCTC	3 cut(s)	359, 844, 977
BsmBI	CGTCTC	2 cut(s)	359, 977
BsmFI	GGGAC	2 cut(s)	507, 748
BsnI	GGCC	4 cut(s)	318, 377, 689, 1211
Bso31I	GGTCTC	1 cut(s)	844
BsoBI	CYCGRG	2 cut(s)	788, 1119
Bsp1286I	GDGCHC	3 cut(s)	355, 401, 1141
Bsp13I	TCCGGA	2 cut(s)	668, 963
Bsp143I	GATC	5 cut(s)	3, 330, 871, 892, 1080
Bsp1720I	GCTNAGC	1 cut(s)	1270
BspANI	GGCC	4 cut(s)	318, 377, 689, 1211
BspCNI	CTCAG	2 cut(s)	24, 592
BspEI	TCCGGA	2 cut(s)	668, 963
BspFNI	CGCG	2 cut(s)	477, 510
BspLI	GGNNCC	4 cut(s)	28, 63, 352, 643
BspMI	ACCTGC	1 cut(s)	629
BspPI	GGATC	3 cut(s)	338, 879, 1088
BspT107I	GGYRCC	1 cut(s)	641
BspTNI	GGTCTC	1 cut(s)	844
BsrFI	RCCGGY	1 cut(s)	1170
BsrI	ACTGG	2 cut(s)	519, 1223
BssAI	RCCGGY	1 cut(s)	1170
BssECI	CCNNGG	5 cut(s)	313, 354, 455, 789, 885
BssMI	GATC	5 cut(s)	3, 330, 871, 892, 1080
BssNI	GRCGYC	1 cut(s)	323
BssT1I	CCWWGG	1 cut(s)	885
Bst2UI	CCWGG	4 cut(s)	148, 314, 320, 930
Bst4CI	ACNGT	2 cut(s)	175, 1094
Bst6I	CTCTTC	2 cut(s)	37, 678
BstACI	GRCGYC	1 cut(s)	323
BstC8I	GCNNGC	3 cut(s)	676, 1114, 1149
BstDEI	CTNAG	4 cut(s)	11, 579, 1270, 1275
BstF5I	GGATG	3 cut(s)	448, 490, 799
BstFNI	CGCG	2 cut(s)	477, 510
BstHHI	GCGC	2 cut(s)	477, 510
BstKTI	GATC	5 cut(s)	6, 333, 874, 895, 1083
BstMAI	GTCTC	3 cut(s)	359, 844, 977
BstMBI	GATC	5 cut(s)	3, 330, 871, 892, 1080
BstNI	CCWGG	4 cut(s)	148, 314, 320, 930
BstSCI	CCNGG	8 cut(s)	146, 312, 318, 354, 401, 454, 523, 928
BstSLI	GKGCMC	1 cut(s)	1141
BstUI	CGCG	2 cut(s)	477, 510
BstV1I	GCAGC	2 cut(s)	484, 562
BstX2I	RGATCY	1 cut(s)	892
BstYI	RGATCY	1 cut(s)	892
BsuRI	GGCC	4 cut(s)	318, 377, 689, 1211
BtsCI	GGATG	3 cut(s)	448, 490, 799
BtsIMutI	CAGTG	1 cut(s)	171
BveI	ACCTGC	1 cut(s)	629
Cac8I	GCNNGC	3 cut(s)	676, 1114, 1149
CfoI	GCGC	2 cut(s)	477, 510
Cfr10I	RCCGGY	1 cut(s)	1170
Cfr13I	GGNCC	2 cut(s)	316, 1209
CseI	GACGC	2 cut(s)	222, 312
CviAII	CATG	2 cut(s)	58, 679
DdeI	CTNAG	4 cut(s)	11, 579, 1270, 1275
DpnI	GATC	5 cut(s)	5, 332, 873, 894, 1082
DpnII	GATC	5 cut(s)	3, 330, 871, 892, 1080
DrdI	GACNNNNNNGTC	1 cut(s)	280
DseDI	GACNNNNNNGTC	1 cut(s)	280
Eam1104I	CTCTTC	2 cut(s)	37, 678
EarI	CTCTTC	2 cut(s)	37, 678
EciI	GGCGGA	4 cut(s)	71, 234, 358, 509
Ecl136II	GAGCTC	1 cut(s)	399
Eco130I	CCWWGG	1 cut(s)	885
Eco147I	AGGCCT	1 cut(s)	689
Eco24I	GRGCYC	2 cut(s)	355, 401
Eco31I	GGTCTC	1 cut(s)	844
Eco32I	GATATC	1 cut(s)	935
Eco53kI	GAGCTC	1 cut(s)	399
Eco57I	CTGAAG	2 cut(s)	18, 735
Eco88I	CYCGRG	2 cut(s)	788, 1119
EcoICRI	GAGCTC	1 cut(s)	399
EcoRII	CCWGG	4 cut(s)	146, 312, 318, 928
EcoRV	GATATC	1 cut(s)	935
EcoT14I	CCWWGG	1 cut(s)	885
EcoT38I	GRGCYC	2 cut(s)	355, 401
ErhI	CCWWGG	1 cut(s)	885
Esp3I	CGTCTC	2 cut(s)	359, 977
FaeI	CATG	2 cut(s)	61, 682
FaqI	GGGAC	2 cut(s)	507, 748
FatI	CATG	2 cut(s)	57, 678
FauI	CCCGC	1 cut(s)	538
Fnu4HI	GCNGC	8 cut(s)	341, 372, 375, 473, 543, 576, 1020, 1200
FokI	GGATG	3 cut(s)	455, 497, 806
FriOI	GRGCYC	2 cut(s)	355, 401
Fsp4HI	GCNGC	8 cut(s)	341, 372, 375, 473, 543, 576, 1020, 1200
FspBI	CTAG	1 cut(s)	1053
GlaI	GCGC	2 cut(s)	476, 509
GluI	GCNGC	8 cut(s)	341, 372, 375, 473, 543, 576, 1020, 1200
GsaI	CCCAGC	1 cut(s)	1052
HaeIII	GGCC	4 cut(s)	318, 377, 689, 1211
HgaI	GACGC	2 cut(s)	222, 312
HhaI	GCGC	2 cut(s)	477, 510
Hin1I	GRCGYC	1 cut(s)	323
Hin1II	CATG	2 cut(s)	61, 682
Hin6I	GCGC	2 cut(s)	475, 508
HinP1I	GCGC	2 cut(s)	475, 508
HinfI	GANTC	5 cut(s)	423, 448, 881, 967, 1030
HphI	GGTGA	4 cut(s)	39, 86, 136, 445
Hpy166II	GTNNAC	1 cut(s)	1067
Hpy188I	TCNGA	4 cut(s)	14, 415, 746, 824
Hpy8I	GTNNAC	1 cut(s)	1067
Hpy99I	CGWCG	4 cut(s)	238, 277, 301, 328
HpyAV	CCTTC	3 cut(s)	109, 424, 475
HpyCH4III	ACNGT	2 cut(s)	175, 1094
HpyCH4V	TGCA	2 cut(s)	905, 941
HpyF3I	CTNAG	4 cut(s)	11, 579, 1270, 1275
Hsp92I	GRCGYC	1 cut(s)	323
Hsp92II	CATG	2 cut(s)	61, 682
HspAI	GCGC	2 cut(s)	475, 508
Kpn2I	TCCGGA	2 cut(s)	668, 963
Kzo9I	GATC	5 cut(s)	3, 330, 871, 892, 1080
LmnI	GCTCC	8 cut(s)	26, 67, 247, 350, 404, 469, 671, 846
Lsp1109I	GCAGC	2 cut(s)	484, 562
LweI	GCATC	2 cut(s)	784, 1186
MaeI	CTAG	1 cut(s)	1053
MaeIII	GTNAC	2 cut(s)	142, 832
MalI	GATC	5 cut(s)	5, 332, 873, 894, 1082
MboI	GATC	5 cut(s)	3, 330, 871, 892, 1080
MboII	GAAGA	9 cut(s)	24, 289, 695, 728, 752, 902, 909, 1166, 1253
MflI	RGATCY	1 cut(s)	892
MhlI	GDGCHC	3 cut(s)	355, 401, 1141
MluCI	AATT	3 cut(s)	705, 1074, 1132
MlyI	GAGTC	1 cut(s)	875
MmeI	TCCRAC	2 cut(s)	769, 1242
MroI	TCCGGA	2 cut(s)	668, 963
MseI	TTAA	3 cut(s)	123, 900, 1131
MslI	CAYNNNNRTG	1 cut(s)	570
MspR9I	CCNGG	8 cut(s)	148, 314, 320, 356, 403, 456, 525, 930
MvaI	CCWGG	4 cut(s)	148, 314, 320, 930
MvnI	CGCG	2 cut(s)	477, 510
NciI	CCSGG	4 cut(s)	356, 403, 456, 525
NdeII	GATC	5 cut(s)	3, 330, 871, 892, 1080
NlaIII	CATG	2 cut(s)	61, 682
NlaIV	GGNNCC	4 cut(s)	28, 63, 352, 643
NmuCI	GTSAC	1 cut(s)	142
PaeR7I	CTCGAG	1 cut(s)	1119
PaqCI	CACCTGC	1 cut(s)	629
PceI	AGGCCT	1 cut(s)	689
PcsI	WCGNNNNNNNCGW	1 cut(s)	281
PfeI	GAWTC	4 cut(s)	423, 448, 967, 1030
PflMI	CCANNNNNTGG	1 cut(s)	626
PfoI	TCCNGGA	2 cut(s)	401, 928
PkrI	GCNGC	8 cut(s)	342, 373, 376, 474, 544, 577, 1021, 1201
PleI	GAGTC	1 cut(s)	875
PpsI	GAGTC	1 cut(s)	875
PshBI	ATTAAT	1 cut(s)	900
Psp124BI	GAGCTC	1 cut(s)	401
Psp6I	CCWGG	4 cut(s)	146, 312, 318, 928
PspFI	CCCAGC	1 cut(s)	1048
PspGI	CCWGG	4 cut(s)	146, 312, 318, 928
PspN4I	GGNNCC	4 cut(s)	28, 63, 352, 643
PspPI	GGNCC	2 cut(s)	316, 1209
PsuI	RGATCY	1 cut(s)	892
RseI	CAYNNNNRTG	1 cut(s)	570
SacI	GAGCTC	1 cut(s)	401
SaqAI	TTAA	3 cut(s)	123, 900, 1131
SatI	GCNGC	8 cut(s)	341, 372, 375, 473, 543, 576, 1020, 1200
Sau3AI	GATC	5 cut(s)	3, 330, 871, 892, 1080
Sau96I	GGNCC	2 cut(s)	316, 1209
SchI	GAGTC	1 cut(s)	875
ScrFI	CCNGG	8 cut(s)	148, 314, 320, 356, 403, 456, 525, 930
SduI	GDGCHC	3 cut(s)	355, 401, 1141
SfaNI	GCATC	2 cut(s)	784, 1186
Sfr274I	CTCGAG	1 cut(s)	1119
SlaI	CTCGAG	1 cut(s)	1119
SmiMI	CAYNNNNRTG	1 cut(s)	570
SmlI	CTYRAG	3 cut(s)	182, 1119, 1142
SmoI	CTYRAG	3 cut(s)	182, 1119, 1142
Sse9I	AATT	3 cut(s)	705, 1074, 1132
SseBI	AGGCCT	1 cut(s)	689
SspMI	CTAG	1 cut(s)	1053
SstI	GAGCTC	1 cut(s)	401
StuI	AGGCCT	1 cut(s)	689
StyD4I	CCNGG	8 cut(s)	146, 312, 318, 354, 401, 454, 523, 928
StyI	CCWWGG	1 cut(s)	885
TaaI	ACNGT	2 cut(s)	175, 1094
TaqI	TCGA	6 cut(s)	326, 763, 774, 1057, 1120, 1239
TaqII	GACCGA	1 cut(s)	795
TasI	AATT	3 cut(s)	705, 1074, 1132
TauI	GCSGC	6 cut(s)	343, 374, 377, 545, 1022, 1202
TfiI	GAWTC	4 cut(s)	423, 448, 967, 1030
Tru1I	TTAA	3 cut(s)	123, 900, 1131
Tru9I	TTAA	3 cut(s)	123, 900, 1131
TscAI	CASTG	1 cut(s)	178
TseFI	GTSAC	1 cut(s)	142
TseI	GCWGC	2 cut(s)	472, 575
Tsp45I	GTSAC	1 cut(s)	142
TspDTI	ATGAA	1 cut(s)	621
TspGWI	ACGGA	1 cut(s)	370
TspRI	CASTG	1 cut(s)	178
Van91I	CCANNNNNTGG	1 cut(s)	626
VspI	ATTAAT	1 cut(s)	900
XapI	RAATTY	1 cut(s)	705
XhoI	CTCGAG	1 cut(s)	1119
XspI	CTAG	1 cut(s)	1053

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.