FvH4_5g30050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009839
Physical Location & Seq
Reverse (-)
20971357 .. 20976223
4867 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30050.t1

Sequence Viewer

Length: 1521 bp
ATGGCACCTGCCCAACCATTTACAGACATTGACCAAGACTGTCAACCATGTATCAATGCAAAACTTGAACTCGATACAGAGAACAGAACTTCCCTAGAAAACTCATCTTTGGAGGACAGAAATTGCGTGACTAACTGTGATGACACTTCCATTAGGATGGAAGCTGTCCTGAATGGAAGAGACGAGGCTTTGATGTGCATTGCGGATGTGGATGTTGACATAGTTGAATGCAGAAATAATCATAACCACAGCTTGCTGGCTGAAACTGAAGACCCGGATGCAACTGAATATTCAAGCTCCTTTGCAGACACTGAGTCTGACACTGAGAATTTTTCTGGATTTAGTGAAGCTGAAGTGGAATCCCAGTTCTTTCCGGAAAATGGTTTGACATCTGCCTTTAATGCATTTAGTAATGCATTTCAAATGAGGAAGAAGAAGTTGACAAATCACTGGAGGACCTTTATACGTCCTGTAATGTGGCGCTGCAAATGGATGGAGTTGAGAATTAAAGAAATTGAATCACAAGAATTGAATTATTCCCGGGCACTTGCAGCATATGATCAGAGAAAACATTCAGCATTTGATCAGTTTATATTAGAAGAATCTGGTTCAAAGTCATTGCCGTTTTCTAGTCAGTGCCTACAAATAAAAGCTACTAAGCGGAGGAAACGCAAGAGAATTGAAGAGACAACTGATATTGCATCTTATATATCACGTCATAACGCATTTTCCTATTTTGAAAATAAGAGATCCGATCCTGATAGCACATCTCTGGCTGACGAATTTAGTAATGCAGTAATCACAGAACACAGCGCTGATCACAATGATAAGCTCAGCACAGCCGATGAGTGGTCGCTCCTTGAGTTTCGAGATGGTGACAAGTCCTTGGAGCACGTCCTTTGGAAAATTGAGACGCTGCACTCTTGGGTTCACAAGCTGAAGAATCAAATTGATGTGGTGATGCCCAAAACTGCTGCTATATTTTCTTCCTCTGAGAATTTGAGCGTTCTTGTACCCTGTGATGGACAGGCCAGCTCTGCCCATAGTCCGGCTTTCTCTGCTGGCAATGGAGATGGAATATATGCCACAACTCAGCAAACTTCAGAGTTCAATATTGGGGATCTAGTCATGCCTGAAAGTGTAGTTTCCAGTTTCGGGGAGGCTATGCCTGCTTCTGATATAATAGAAAGCTCTGTGGGACTACTGTCTGCTACTGATGTCATCTTACATCAGCCGCAGTTTAGTGAATCAAGTGAAGATATTGTGGATAATGTCTTGATATACAATGCGGCAGAAGGAGAGAAGCTCATGGTCCGAAGGATGGGAGATGAAACCAAAGAGAACCATCAGCAACAACCAGAGGAAGCCAAACAAGGTCCTTGTGCAACTCCTTCATCAACTGTAGAATCATGCTTAGGTTTAGATGTAAACTTTCCAAGAAACAAGAGAAAAAGAGGGGAGCGAAAAACCGGTTCAGATGTTTGGAGCAAGAAATGCTCAGGTGAGCCTGGTAGCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

507

Amino Acids

56.67

Weight (kDa)

4.81

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 16
Acc36I ACCTGC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 4
AccIII TCCGGA 1 cut(s) 373
AciI CCGC 4 cut(s) 203, 661, 1235, 1289
AclWI GGATC 3 cut(s) 744, 749, 1128
AcsI RAATTY 3 cut(s) 328, 782, 997
AcuI CTGAAG 4 cut(s) 288, 372, 959, 1086
AfaI GTAC 1 cut(s) 1014
AfeI AGCGCT 1 cut(s) 814
AfiI CCNNNNNNNGG 6 cut(s) 380, 849, 1022, 1048, 1155, 1321
AgeI ACCGGT 1 cut(s) 1469
AhdI GACNNNNNGTC 1 cut(s) 313
AjiI CACGTC 2 cut(s) 716, 895
AjnI CCWGG 1 cut(s) 1507
AloI GAACNNNNNNTCC 4 cut(s) 74, 106, 350, 382
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 3 cut(s) 174, 680, 905
AlwI GGATC 3 cut(s) 744, 749, 1128
AlwNI CAGNNNCTG 1 cut(s) 311
Ama87I CYCGRG 1 cut(s) 540
Aor13HI TCCGGA 1 cut(s) 373
Aor51HI AGCGCT 1 cut(s) 814
AoxI GGCC 1 cut(s) 1029
ApeKI GCWGC 4 cut(s) 483, 551, 916, 974
ApoI RAATTY 3 cut(s) 328, 782, 997
AsiGI ACCGGT 1 cut(s) 1469
Asp700I GAANNNNTTC 1 cut(s) 571
AspLEI GCGC 2 cut(s) 483, 815
AspS9I GGNCC 3 cut(s) 456, 1312, 1376
AsuC2I CCSGG 3 cut(s) 275, 541, 542
AsuHPI GGTGA 3 cut(s) 887, 970, 1514
AvaI CYCGRG 1 cut(s) 540
AvaII GGWCC 3 cut(s) 456, 1312, 1376
BaeGI GKGCMC 1 cut(s) 547
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 894
BbvI GCAGC 4 cut(s) 470, 563, 903, 961
BccI CCATC 7 cut(s) 151, 487, 866, 1016, 1067, 1315, 1353
BceAI ACGGC 1 cut(s) 607
BciT130I CCWGG 1 cut(s) 1509
BclI TGATCA 3 cut(s) 559, 583, 817
BcnI CCSGG 3 cut(s) 275, 541, 542
BcoDI GTCTC 3 cut(s) 174, 680, 905
BfaI CTAG 3 cut(s) 95, 630, 1124
BfmI CTRYAG 1 cut(s) 1401
BfoI RGCGCY 2 cut(s) 484, 816
BfuAI ACCTGC 1 cut(s) 16
BisI GCNGC 6 cut(s) 484, 552, 917, 975, 1235, 1290
BlpI GCTNAGC 1 cut(s) 833
BlsI GCNGC 6 cut(s) 485, 553, 918, 976, 1236, 1291
Bme1390I CCNGG 4 cut(s) 275, 541, 542, 1509
Bme18I GGWCC 3 cut(s) 456, 1312, 1376
BmeRI GACNNNNNGTC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 540
BmgBI CACGTC 2 cut(s) 716, 895
BmgT120I GGNCC 3 cut(s) 456, 1312, 1376
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 4 cut(s) 275, 541, 542, 1509
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 3 cut(s) 268, 710, 951
BmuI ACTGGG 1 cut(s) 358
BoxI GACNNNNGTC 1 cut(s) 1204
BpiI GAAGAC 1 cut(s) 276
BplI GAGNNNNNCTC 2 cut(s) 1290, 1322
BpmI CTGGAG 1 cut(s) 472
Bpu10I CCTNAGC 2 cut(s) 1414, 1498
Bpu1102I GCTNAGC 1 cut(s) 833
BpuEI CTTGAG 1 cut(s) 881
BpuMI CCSGG 3 cut(s) 275, 541, 542
BsaJI CCNNGG 2 cut(s) 540, 885
BsaWI WCCGGW 2 cut(s) 373, 1469
Bsc4I CCNNNNNNNGG 6 cut(s) 380, 849, 1022, 1048, 1155, 1321
Bse118I RCCGGY 1 cut(s) 1469
Bse1I ACTGG 3 cut(s) 364, 455, 1149
Bse3DI GCAATG 3 cut(s) 198, 617, 1072
BseAI TCCGGA 1 cut(s) 373
BseBI CCWGG 1 cut(s) 1509
BseDI CCNNGG 2 cut(s) 540, 885
BseGI GGATG 6 cut(s) 162, 211, 217, 283, 498, 1326
BseLI CCNNNNNNNGG 6 cut(s) 380, 849, 1022, 1048, 1155, 1321
BseMI GCAATG 3 cut(s) 198, 617, 1072
BseMII CTCAG 6 cut(s) 303, 315, 847, 984, 1106, 1512
BseNI ACTGG 3 cut(s) 364, 455, 1149
BseSI GKGCMC 1 cut(s) 547
BseXI GCAGC 4 cut(s) 470, 563, 903, 961
BsgI GTGCAG 1 cut(s) 902
BshFI GGCC 1 cut(s) 1031
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 1469
BsiHKAI GWGCWC 1 cut(s) 894
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 5 cut(s) 275, 374, 541, 1049, 1470
BslFI GGGAC 1 cut(s) 1212
BslI CCNNNNNNNGG 6 cut(s) 380, 849, 1022, 1048, 1155, 1321
BsmAI GTCTC 3 cut(s) 174, 680, 905
BsmBI CGTCTC 2 cut(s) 174, 905
BsmFI GGGAC 1 cut(s) 1212
BsmI GAATGC 1 cut(s) 233
BsnI GGCC 1 cut(s) 1031
BsoBI CYCGRG 1 cut(s) 540
Bsp1286I GDGCHC 2 cut(s) 547, 894
Bsp13I TCCGGA 1 cut(s) 373
Bsp143I GATC 6 cut(s) 559, 583, 749, 754, 817, 1120
Bsp1720I GCTNAGC 1 cut(s) 833
BspACI CCGC 4 cut(s) 203, 661, 1235, 1289
BspANI GGCC 1 cut(s) 1031
BspCNI CTCAG 6 cut(s) 304, 316, 846, 985, 1105, 1511
BspEI TCCGGA 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 16
BspPI GGATC 3 cut(s) 744, 749, 1128
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 3 cut(s) 198, 617, 1072
BsrFI RCCGGY 1 cut(s) 1469
BsrI ACTGG 3 cut(s) 364, 455, 1149
BssAI RCCGGY 1 cut(s) 1469
BssECI CCNNGG 2 cut(s) 540, 885
BssMI GATC 6 cut(s) 559, 583, 749, 754, 817, 1120
BssT1I CCWWGG 1 cut(s) 885
Bst2UI CCWGG 1 cut(s) 1509
Bst4CI ACNGT 4 cut(s) 41, 137, 1206, 1402
Bst6I CTCTTC 2 cut(s) 172, 678
BstAPI GCANNNNNTGC 1 cut(s) 1494
BstC8I GCNNGC 5 cut(s) 254, 258, 1033, 1063, 1170
BstDEI CTNAG 8 cut(s) 312, 324, 657, 833, 993, 1092, 1414, 1498
BstF5I GGATG 6 cut(s) 162, 211, 217, 283, 498, 1326
BstH2I RGCGCY 2 cut(s) 484, 816
BstHHI GCGC 2 cut(s) 483, 815
BstKTI GATC 6 cut(s) 562, 586, 752, 757, 820, 1123
BstMAI GTCTC 3 cut(s) 174, 680, 905
BstMBI GATC 6 cut(s) 559, 583, 749, 754, 817, 1120
BstMWI GCNNNNNNNGC 6 cut(s) 401, 551, 1037, 1058, 1169, 1494
BstNI CCWGG 1 cut(s) 1509
BstPAI GACNNNNGTC 1 cut(s) 1204
BstSCI CCNGG 4 cut(s) 273, 539, 540, 1507
BstSFI CTRYAG 1 cut(s) 1401
BstSLI GKGCMC 1 cut(s) 547
BstV1I GCAGC 4 cut(s) 470, 563, 903, 961
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 2 cut(s) 749, 1120
BstXI CCANNNNNNTGG 1 cut(s) 157
BstYI RGATCY 2 cut(s) 749, 1120
BsuRI GGCC 1 cut(s) 1031
BtrI CACGTC 2 cut(s) 716, 895
BtsCI GGATG 6 cut(s) 162, 211, 217, 283, 498, 1326
BtsIMutI CAGTG 4 cut(s) 309, 321, 448, 641
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 5 cut(s) 254, 258, 1033, 1063, 1170
CaiI CAGNNNCTG 1 cut(s) 311
CfoI GCGC 2 cut(s) 483, 815
Cfr10I RCCGGY 1 cut(s) 1469
Cfr13I GGNCC 3 cut(s) 456, 1312, 1376
Cfr9I CCCGGG 1 cut(s) 540
CseI GACGC 1 cut(s) 922
Csp6I GTAC 1 cut(s) 1013
CspAI ACCGGT 1 cut(s) 1469
CviAII CATG 4 cut(s) 48, 1129, 1309, 1410
CviQI GTAC 1 cut(s) 1013
DdeI CTNAG 8 cut(s) 312, 324, 657, 833, 993, 1092, 1414, 1498
DpnI GATC 6 cut(s) 561, 585, 751, 756, 819, 1122
DpnII GATC 6 cut(s) 559, 583, 749, 754, 817, 1120
DriI GACNNNNNGTC 1 cut(s) 313
Eam1104I CTCTTC 2 cut(s) 172, 678
Eam1105I GACNNNNNGTC 1 cut(s) 313
EarI CTCTTC 2 cut(s) 172, 678
Eco130I CCWWGG 1 cut(s) 885
Eco47I GGWCC 3 cut(s) 456, 1312, 1376
Eco47III AGCGCT 1 cut(s) 814
Eco57I CTGAAG 4 cut(s) 288, 372, 959, 1086
Eco88I CYCGRG 1 cut(s) 540
EcoO109I RGGNCCY 2 cut(s) 456, 1376
EcoRII CCWGG 1 cut(s) 1507
EcoT14I CCWWGG 1 cut(s) 885
EcoT22I ATGCAT 2 cut(s) 406, 418
ErhI CCWWGG 1 cut(s) 885
Esp3I CGTCTC 2 cut(s) 174, 905
FaeI CATG 4 cut(s) 51, 1132, 1312, 1413
FaqI GGGAC 1 cut(s) 1212
FatI CATG 4 cut(s) 47, 1128, 1308, 1409
FauNDI CATATG 1 cut(s) 556
FbaI TGATCA 3 cut(s) 559, 583, 817
Fnu4HI GCNGC 6 cut(s) 484, 552, 917, 975, 1235, 1290
FokI GGATG 6 cut(s) 169, 218, 224, 290, 505, 1333
Fsp4HI GCNGC 6 cut(s) 484, 552, 917, 975, 1235, 1290
FspBI CTAG 3 cut(s) 95, 630, 1124
GlaI GCGC 2 cut(s) 482, 814
GluI GCNGC 6 cut(s) 484, 552, 917, 975, 1235, 1290
GsuI CTGGAG 1 cut(s) 472
HaeII RGCGCY 2 cut(s) 484, 816
HaeIII GGCC 1 cut(s) 1031
HapII CCGG 5 cut(s) 275, 374, 541, 1049, 1470
HgaI GACGC 1 cut(s) 922
HhaI GCGC 2 cut(s) 483, 815
Hin1II CATG 4 cut(s) 51, 1132, 1312, 1413
Hin6I GCGC 2 cut(s) 481, 813
HinP1I GCGC 2 cut(s) 481, 813
HincII GTYRAC 3 cut(s) 44, 217, 441
HindII GTYRAC 3 cut(s) 44, 217, 441
HinfI GANTC 7 cut(s) 314, 359, 518, 602, 943, 1247, 1406
HpaII CCGG 5 cut(s) 275, 374, 541, 1049, 1470
HphI GGTGA 3 cut(s) 887, 970, 1514
Hpy166II GTNNAC 5 cut(s) 44, 217, 441, 931, 1429
Hpy188I TCNGA 8 cut(s) 319, 564, 754, 994, 1105, 1177, 1316, 1477
Hpy188III TCNNGA 6 cut(s) 169, 336, 374, 758, 869, 1276
Hpy8I GTNNAC 5 cut(s) 44, 217, 441, 931, 1429
HpyAV CCTTC 3 cut(s) 1289, 1311, 1401
HpyCH4III ACNGT 4 cut(s) 41, 137, 1206, 1402
HpyCH4IV ACGT 3 cut(s) 466, 715, 894
HpyF10VI GCNNNNNNNGC 6 cut(s) 401, 551, 1037, 1058, 1169, 1494
HpyF3I CTNAG 8 cut(s) 312, 324, 657, 833, 993, 1092, 1414, 1498
HpySE526I ACGT 3 cut(s) 466, 715, 894
Hsp92II CATG 4 cut(s) 51, 1132, 1312, 1413
HspAI GCGC 2 cut(s) 481, 813
Kpn2I TCCGGA 1 cut(s) 373
Ksp22I TGATCA 3 cut(s) 559, 583, 817
Kzo9I GATC 6 cut(s) 559, 583, 749, 754, 817, 1120
LmnI GCTCC 5 cut(s) 302, 861, 889, 1459, 1485
Lsp1109I GCAGC 4 cut(s) 470, 563, 903, 961
LweI GCATC 3 cut(s) 268, 710, 951
MaeI CTAG 3 cut(s) 95, 630, 1124
MaeII ACGT 3 cut(s) 466, 715, 894
MaeIII GTNAC 2 cut(s) 127, 875
MalI GATC 6 cut(s) 561, 585, 751, 756, 819, 1122
MboI GATC 6 cut(s) 559, 583, 749, 754, 817, 1120
MboII GAAGA 9 cut(s) 189, 281, 442, 445, 611, 695, 952, 978, 1268
MflI RGATCY 2 cut(s) 749, 1120
MhlI GDGCHC 2 cut(s) 547, 894
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 9 cut(s) 106, 178, 420, 447, 657, 1000, 1153, 1354, 1448
Mph1103I ATGCAT 2 cut(s) 406, 418
MroI TCCGGA 1 cut(s) 373
MroXI GAANNNNTTC 1 cut(s) 571
MseI TTAA 2 cut(s) 399, 507
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 5 cut(s) 275, 374, 541, 1049, 1470
MspR9I CCNGG 4 cut(s) 275, 541, 542, 1509
Mva1269I GAATGC 1 cut(s) 233
MvaI CCWGG 1 cut(s) 1509
MwoI GCNNNNNNNGC 6 cut(s) 401, 551, 1037, 1058, 1169, 1494
NciI CCSGG 3 cut(s) 275, 541, 542
NdeI CATATG 1 cut(s) 556
NdeII GATC 6 cut(s) 559, 583, 749, 754, 817, 1120
NlaIII CATG 4 cut(s) 51, 1132, 1312, 1413
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 127, 875
NsiI ATGCAT 2 cut(s) 406, 418
PaqCI CACCTGC 1 cut(s) 16
PctI GAATGC 1 cut(s) 233
PdmI GAANNNNTTC 1 cut(s) 571
PfeI GAWTC 6 cut(s) 359, 518, 602, 943, 1247, 1406
PinAI ACCGGT 1 cut(s) 1469
PkrI GCNGC 6 cut(s) 485, 553, 918, 976, 1236, 1291
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PpuMI RGGWCCY 2 cut(s) 456, 1376
PshAI GACNNNNGTC 1 cut(s) 1204
Psp5II RGGWCCY 2 cut(s) 456, 1376
Psp6I CCWGG 1 cut(s) 1507
PspGI CCWGG 1 cut(s) 1507
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 456, 1312, 1376
PspPPI RGGWCCY 2 cut(s) 456, 1376
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 2 cut(s) 749, 1120
RsaI GTAC 1 cut(s) 1014
RsaNI GTAC 1 cut(s) 1013
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 2 cut(s) 399, 507
SatI GCNGC 6 cut(s) 484, 552, 917, 975, 1235, 1290
Sau3AI GATC 6 cut(s) 559, 583, 749, 754, 817, 1120
Sau96I GGNCC 3 cut(s) 456, 1312, 1376
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 4 cut(s) 275, 541, 542, 1509
SduI GDGCHC 2 cut(s) 547, 894
SfaNI GCATC 3 cut(s) 268, 710, 951
SfcI CTRYAG 1 cut(s) 1401
SinI GGWCC 3 cut(s) 456, 1312, 1376
SmaI CCCGGG 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
SsiI CCGC 4 cut(s) 203, 661, 1235, 1289
SspI AATATT 2 cut(s) 290, 1114
SspMI CTAG 3 cut(s) 95, 630, 1124
StyD4I CCNGG 4 cut(s) 273, 539, 540, 1507
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 4 cut(s) 41, 137, 1206, 1402
TaiI ACGT 3 cut(s) 469, 718, 897
TaqI TCGA 2 cut(s) 72, 868
TauI GCSGC 2 cut(s) 1237, 1292
TfiI GAWTC 6 cut(s) 359, 518, 602, 943, 1247, 1406
Tru1I TTAA 2 cut(s) 399, 507
Tru9I TTAA 2 cut(s) 399, 507
TscAI CASTG 4 cut(s) 316, 328, 455, 641
TseFI GTSAC 2 cut(s) 127, 875
TseI GCWGC 4 cut(s) 483, 551, 916, 974
Tsp45I GTSAC 2 cut(s) 127, 875
TspDTI ATGAA 2 cut(s) 1344, 1383
TspMI CCCGGG 1 cut(s) 540
TspRI CASTG 4 cut(s) 316, 328, 455, 641
VpaK11BI GGWCC 3 cut(s) 456, 1312, 1376
XapI RAATTY 3 cut(s) 328, 782, 997
XmaI CCCGGG 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 571
XspI CTAG 3 cut(s) 95, 630, 1124
Zsp2I ATGCAT 2 cut(s) 406, 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.