Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009839

Physical Location & Seq

Genomic Coordinates Forward (+)

5760084 .. 5763930

Gene Structure 3847 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1049200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGCTCCTGCCCAACCAGACATGGAGGCTGACCAGCTCCAAGACTGCAAACCATTTGTCAACCCAAAAGTTGAACTTGTTGAGGATGATACAGATATCAGAAGGGCTTCCTTGCCAAACTCTGCTTCTGCTTCGGCTTCGGCATCGGAGGGAAAACACATCGCTCCAGTCGTCGACATTGATATAATTCAGTGCACAAGTAATCAGGACAGTACTCAGCCTGATCCCGATGCAACGGATGAATATTCAACCTCATTCGCCGACACTACGTCCGACACTGACAATTTTTCTGGATTTTGTGAAGAAGGTGAAGTGCAGTCACACGAGCACAATGCTTTGACATCTGCTTTTGATGCATTTGCGAGTACATTTCAAACCAGGAAGAAGAAGTTGACAAATCACTGGAGGAGCTTTATACGTCCTGTAATGTGGCGCTGCAAATGGATGGAATTGAGAATCAAAGAAATTGAAGCACAAGCACTAAAGTATTCCAGAGACCTACGAGTAGCATATGATAAAAAAAGGCATACAGGATTCGACCAACGTACTTTGGATGAATTTGGTTCAAAGTCATTGCCATTTTGTAGTCAATTCCGCAGAAAGAAAGCTATGAAGAGGAGGAAACGCAAGAGAGTTGAAGACACAACTGATATAGCATCATACATCTCAAATCATAGTGTCTTTTCCTATCTTGAAAATAAAAGATCCGATCCTGACAGCACATCCATTGCTGATGACTTTAATAATGCAGTAATCACAGAGCAGAGTGCGGATTACAATGATAAACTCAGTGCTGGTGATAACTGGGCATTCTTTGAGTACCGAGATGGTGACAAGTCTTTGGAGCATGTCCTTTGGAAAATTGAGACAGTGCACTCTCGGGTTCACAAGTTAAAGTATCAAATGGATGTGGTGATGTCAAAAAATGCTGCAAGGTTTTCTTCCAGCGAAAATTTGAGCATTCTTGTACCCTGTGATGCACAGACCAGCTCTGCACATAGTCCAGCATTGTCTCCTGGCAATGGAGAAACAAACTCTGCGGGAGCTATATATACTTCAGCTCAACATGTATCAGGCTACAATGTTGGGGATCTCGCAATGCCTGAAAGTGCAGTCTCAAGTTTTGTGGAGGGAGTCACTGTTCCTGATATAATTGAAAGCACTGTGGGACTACCGTCTGCTGTTGACGTTACCTTCCATCAGCCACAGTTTGGAGACTCAAGCGAAGATATTGTGGATAATGTCCTGATACATAATGAGGCAGCTGAAGCAGAGAAGCGCGCTTTCCAAATGATCAACGACCAACCTAAAGAGGAACATCAGCAACCAGAGAAAGACTTACAAGAAGCCGCTTTTCCAACTCCACAATCAGAACCTGCTCCCTCGGCAGAAGCTGCTGTTCCTCAGGAACAATCAACTCTAGAGTCATGCTTGGCTTCCGATGTAAATTTTCATAGAAACAAGAGAAAGCGAGGGGAGCGGAAAGCAGGTTCGGTTGTTTGGAGCAAGAAATGCTCAGGCGAGCCCGATAGCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

513

Amino Acids

57.06

Weight (kDa)

5.13

Isoelectric Point (pI)

53.57

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009839)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59670
fragaria_vesca	FvH4_5g30050
malus_domestica	MD04G1049200.v1.1 MD17G1177900.v1.1
prunus_persica	Prupe.1G207400_v2.0.a1 Prupe.1G207400_v2.0.a1
pyrus_communis	pycom04g04340 pycom17g18680 pycom17g18690
rosa_chinensis	RchiOBHm_Chr7g0224241
rosa_laevigata	RLG00000001911
rosa_multiflora	Rmu_sc0008008.1_g000002 Rmu_sc0023501.1_g000004
rosa_roxburghii	Rroxscaffold_3G00235030
rosa_rugosa	Rorug07G0221600
rosa_samantha	Rh7AG366500 Rh7BG330000 Rh7BG357500 Rh7CG384800 Rh7DG360400
rosa_wichuraiana	Rw7G030990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1384, 1478
AccB7I	CCANNNNNTGG	1 cut(s)	1211
AccBSI	CCGCTC	1 cut(s)	1480
AccI	GTMKAC	1 cut(s)	174
AccII	CGCG	1 cut(s)	1281
AciI	CCGC	5 cut(s)	595, 770, 1040, 1350, 1480
AclWI	GGATC	4 cut(s)	218, 699, 704, 1098
AcsI	RAATTY	3 cut(s)	557, 952, 1447
AcuI	CTGAAG	2 cut(s)	1041, 1287
AfaI	GTAC	5 cut(s)	214, 367, 547, 821, 969
AfiI	CCNNNNNNNGG	2 cut(s)	1022, 1211
AflIII	ACRYGT	1 cut(s)	1066
AgsI	TTSAA	8 cut(s)	74, 249, 374, 470, 567, 638, 695, 1157
AhdI	GACNNNNNGTC	1 cut(s)	268
AjnI	CCWGG	2 cut(s)	377, 1015
AjuI	GAANNNNNNNTTGG	2 cut(s)	109, 141
AloI	GAACNNNNNNTCC	4 cut(s)	1125, 1157, 1474, 1506
AluBI	AGCT	8 cut(s)	37, 411, 608, 990, 1046, 1061, 1265, 1394
AluI	AGCT	8 cut(s)	37, 411, 608, 990, 1046, 1061, 1265, 1394
Alw21I	GWGCWC	3 cut(s)	197, 330, 876
Alw26I	GTCTC	5 cut(s)	489, 860, 1017, 1120, 1209
Alw44I	GTGCAC	2 cut(s)	193, 872
AlwI	GGATC	4 cut(s)	218, 699, 704, 1098
AlwNI	CAGNNNCTG	2 cut(s)	1376, 1394
Ama87I	CYCGRG	1 cut(s)	879
ApaLI	GTGCAC	2 cut(s)	193, 872
ApeKI	GCWGC	4 cut(s)	435, 929, 1262, 1394
ApoI	RAATTY	3 cut(s)	557, 952, 1447
ArsI	GACNNNNNNTTYG	2 cut(s)	42, 74
Asp700I	GAANNNNTTC	1 cut(s)	106
AspLEI	GCGC	3 cut(s)	435, 1281, 1283
AsuHPI	GGTGA	4 cut(s)	320, 809, 842, 925
AvaI	CYCGRG	1 cut(s)	879
AxyI	CCTNAGG	1 cut(s)	1404
BaeGI	GKGCMC	2 cut(s)	197, 876
BanII	GRGCYC	1 cut(s)	1527
BauI	CACGAG	1 cut(s)	323
BbsI	GAAGAC	1 cut(s)	645
Bbv12I	GWGCWC	3 cut(s)	197, 330, 876
BbvI	GCAGC	4 cut(s)	422, 916, 1274, 1381
BccI	CCATC	3 cut(s)	439, 821, 1206
BcgI	CGANNNNNNTGC	2 cut(s)	314, 348
BciT130I	CCWGG	2 cut(s)	379, 1017
BclI	TGATCA	1 cut(s)	1293
BcoDI	GTCTC	5 cut(s)	489, 860, 1017, 1120, 1209
BfaI	CTAG	1 cut(s)	1421
BfoI	RGCGCY	1 cut(s)	436
BfuAI	ACCTGC	2 cut(s)	1384, 1478
BisI	GCNGC	5 cut(s)	436, 930, 1263, 1350, 1395
BlsI	GCNGC	5 cut(s)	437, 931, 1264, 1351, 1396
BmcAI	AGTACT	1 cut(s)	214
Bme1390I	CCNGG	2 cut(s)	379, 1017
BmeRI	GACNNNNNGTC	1 cut(s)	268
BmeT110I	CYCGRG	1 cut(s)	879
BmiI	GGNNCC	1 cut(s)	6
BmrFI	CCNGG	2 cut(s)	379, 1017
BmrI	ACTGGG	1 cut(s)	814
BmsI	GCATC	5 cut(s)	152, 220, 343, 665, 967
BmuI	ACTGGG	1 cut(s)	814
BoxI	GACNNNNGTC	1 cut(s)	1174
BpiI	GAAGAC	1 cut(s)	645
BpmI	CTGGAG	2 cut(s)	150, 424
Bpu10I	CCTNAGC	1 cut(s)	1516
BpuEI	CTTGAG	2 cut(s)	1102, 1204
BsaI	GGTCTC	1 cut(s)	489
BsaJI	CCNNGG	1 cut(s)	1383
BsaXI	ACNNNNNCTCC	2 cut(s)	1125, 1155
Bsc4I	CCNNNNNNNGG	2 cut(s)	1022, 1211
Bse1I	ACTGG	3 cut(s)	167, 407, 809
Bse21I	CCTNAGG	1 cut(s)	1404
Bse3DI	GCAATG	4 cut(s)	572, 726, 1027, 1104
BseBI	CCWGG	2 cut(s)	379, 1017
BseDI	CCNNGG	1 cut(s)	1383
BseGI	GGATG	6 cut(s)	91, 244, 450, 559, 722, 913
BseLI	CCNNNNNNNGG	2 cut(s)	1022, 1211
BseMI	GCAATG	4 cut(s)	572, 726, 1027, 1104
BseMII	CTCAG	4 cut(s)	230, 802, 1418, 1530
BseNI	ACTGG	3 cut(s)	167, 407, 809
BsePI	GCGCGC	1 cut(s)	1279
BseRI	GAGGAG	2 cut(s)	421, 631
BseSI	GKGCMC	2 cut(s)	197, 876
BseXI	GCAGC	4 cut(s)	422, 916, 1274, 1381
BsgI	GTGCAG	3 cut(s)	335, 978, 1131
Bsh1236I	CGCG	1 cut(s)	1281
BsiHKAI	GWGCWC	3 cut(s)	197, 330, 876
BsiHKCI	CYCGRG	1 cut(s)	879
BslFI	GGGAC	1 cut(s)	1182
BslI	CCNNNNNNNGG	2 cut(s)	1022, 1211
BsmAI	GTCTC	5 cut(s)	489, 860, 1017, 1120, 1209
BsmFI	GGGAC	1 cut(s)	1182
BsmI	GAATGC	2 cut(s)	809, 960
Bso31I	GGTCTC	1 cut(s)	489
BsoBI	CYCGRG	1 cut(s)	879
Bsp1286I	GDGCHC	4 cut(s)	197, 330, 876, 1527
Bsp143I	GATC	5 cut(s)	223, 704, 709, 1090, 1293
BspACI	CCGC	5 cut(s)	595, 770, 1040, 1350, 1480
BspCNI	CTCAG	4 cut(s)	229, 801, 1417, 1529
BspFNI	CGCG	1 cut(s)	1281
BspLI	GGNNCC	1 cut(s)	6
BspMI	ACCTGC	2 cut(s)	1384, 1478
BspPI	GGATC	4 cut(s)	218, 699, 704, 1098
BspTNI	GGTCTC	1 cut(s)	489
BsrBI	CCGCTC	1 cut(s)	1480
BsrDI	GCAATG	4 cut(s)	572, 726, 1027, 1104
BsrI	ACTGG	3 cut(s)	167, 407, 809
BssECI	CCNNGG	1 cut(s)	1383
BssHII	GCGCGC	1 cut(s)	1279
BssMI	GATC	5 cut(s)	223, 704, 709, 1090, 1293
BssSI	CACGAG	1 cut(s)	323
Bst2BI	CACGAG	1 cut(s)	323
Bst2UI	CCWGG	2 cut(s)	379, 1017
Bst4CI	ACNGT	6 cut(s)	212, 871, 1141, 1165, 1176, 1209
Bst6I	CTCTTC	1 cut(s)	608
BstAPI	GCANNNNNTGC	2 cut(s)	1394, 1512
BstC8I	GCNNGC	2 cut(s)	1281, 1523
BstDEI	CTNAG	4 cut(s)	216, 788, 1404, 1516
BstF5I	GGATG	6 cut(s)	91, 244, 450, 559, 722, 913
BstFNI	CGCG	1 cut(s)	1281
BstH2I	RGCGCY	1 cut(s)	436
BstHHI	GCGC	3 cut(s)	435, 1281, 1283
BstKTI	GATC	5 cut(s)	226, 707, 712, 1093, 1296
BstMAI	GTCTC	5 cut(s)	489, 860, 1017, 1120, 1209
BstMBI	GATC	5 cut(s)	223, 704, 709, 1090, 1293
BstMWI	GCNNNNNNNGC	6 cut(s)	353, 1268, 1385, 1394, 1477, 1512
BstNI	CCWGG	2 cut(s)	379, 1017
BstNSI	RCATGY	2 cut(s)	851, 1070
BstPAI	GACNNNNGTC	1 cut(s)	1174
BstSCI	CCNGG	2 cut(s)	377, 1015
BstSLI	GKGCMC	2 cut(s)	197, 876
BstUI	CGCG	1 cut(s)	1281
BstV1I	GCAGC	4 cut(s)	422, 916, 1274, 1381
BstV2I	GAAGAC	1 cut(s)	645
BstX2I	RGATCY	2 cut(s)	704, 1090
BstYI	RGATCY	2 cut(s)	704, 1090
Bsu36I	CCTNAGG	1 cut(s)	1404
BtgZI	GCGATG	1 cut(s)	145
BtsCI	GGATG	6 cut(s)	91, 244, 450, 559, 722, 913
BtsIMutI	CAGTG	7 cut(s)	197, 276, 400, 796, 876, 1137, 1161
BveI	ACCTGC	2 cut(s)	1384, 1478
Cac8I	GCNNGC	2 cut(s)	1281, 1523
CaiI	CAGNNNCTG	2 cut(s)	1376, 1394
CfoI	GCGC	3 cut(s)	435, 1281, 1283
Csp6I	GTAC	5 cut(s)	213, 366, 546, 820, 968
CspCI	CAANNNNNGTGG	2 cut(s)	1107, 1142
CviAII	CATG	4 cut(s)	22, 848, 1067, 1428
CviQI	GTAC	5 cut(s)	213, 366, 546, 820, 968
DdeI	CTNAG	4 cut(s)	216, 788, 1404, 1516
DpnI	GATC	5 cut(s)	225, 706, 711, 1092, 1295
DpnII	GATC	5 cut(s)	223, 704, 709, 1090, 1293
DriI	GACNNNNNGTC	1 cut(s)	268
Eam1104I	CTCTTC	1 cut(s)	608
Eam1105I	GACNNNNNGTC	1 cut(s)	268
EarI	CTCTTC	1 cut(s)	608
Eco24I	GRGCYC	1 cut(s)	1527
Eco31I	GGTCTC	1 cut(s)	489
Eco32I	GATATC	1 cut(s)	97
Eco57I	CTGAAG	2 cut(s)	1041, 1287
Eco81I	CCTNAGG	1 cut(s)	1404
Eco88I	CYCGRG	1 cut(s)	879
EcoRII	CCWGG	2 cut(s)	377, 1015
EcoRV	GATATC	1 cut(s)	97
EcoT22I	ATGCAT	1 cut(s)	358
EcoT38I	GRGCYC	1 cut(s)	1527
FaeI	CATG	4 cut(s)	25, 851, 1070, 1431
FalI	AAGNNNNNCTT	4 cut(s)	60, 92, 925, 957
FaqI	GGGAC	1 cut(s)	1182
FatI	CATG	4 cut(s)	21, 847, 1066, 1427
FauI	CCCGC	1 cut(s)	1033
FauNDI	CATATG	1 cut(s)	511
FbaI	TGATCA	1 cut(s)	1293
FblI	GTMKAC	1 cut(s)	174
Fnu4HI	GCNGC	5 cut(s)	436, 930, 1263, 1350, 1395
FokI	GGATG	6 cut(s)	98, 251, 457, 566, 709, 920
FriOI	GRGCYC	1 cut(s)	1527
Fsp4HI	GCNGC	5 cut(s)	436, 930, 1263, 1350, 1395
FspBI	CTAG	1 cut(s)	1421
GlaI	GCGC	3 cut(s)	434, 1280, 1282
GluI	GCNGC	5 cut(s)	436, 930, 1263, 1350, 1395
GsuI	CTGGAG	2 cut(s)	150, 424
HaeII	RGCGCY	1 cut(s)	436
HhaI	GCGC	3 cut(s)	435, 1281, 1283
Hin1II	CATG	4 cut(s)	25, 851, 1070, 1431
Hin6I	GCGC	3 cut(s)	433, 1279, 1281
HinP1I	GCGC	3 cut(s)	433, 1279, 1281
HincII	GTYRAC	4 cut(s)	61, 175, 393, 1186
HindII	GTYRAC	4 cut(s)	61, 175, 393, 1186
HinfI	GANTC	5 cut(s)	456, 534, 1134, 1217, 1424
HphI	GGTGA	4 cut(s)	320, 809, 842, 925
Hpy166II	GTNNAC	7 cut(s)	61, 175, 195, 393, 874, 886, 1186
Hpy188I	TCNGA	6 cut(s)	101, 148, 274, 709, 1372, 1441
Hpy8I	GTNNAC	7 cut(s)	61, 175, 195, 393, 874, 886, 1186
Hpy99I	CGWCG	1 cut(s)	176
HpyAV	CCTTC	3 cut(s)	96, 299, 1204
HpyCH4III	ACNGT	6 cut(s)	212, 871, 1141, 1165, 1176, 1209
HpyCH4IV	ACGT	4 cut(s)	269, 418, 544, 1188
HpyF10VI	GCNNNNNNNGC	6 cut(s)	353, 1268, 1385, 1394, 1477, 1512
HpyF3I	CTNAG	4 cut(s)	216, 788, 1404, 1516
HpySE526I	ACGT	4 cut(s)	269, 418, 544, 1188
Hsp92II	CATG	4 cut(s)	25, 851, 1070, 1431
HspAI	GCGC	3 cut(s)	433, 1279, 1281
Ksp22I	TGATCA	1 cut(s)	1293
Kzo9I	GATC	5 cut(s)	223, 704, 709, 1090, 1293
LmnI	GCTCC	9 cut(s)	10, 42, 169, 408, 844, 1043, 1384, 1477, 1503
Lsp1109I	GCAGC	4 cut(s)	422, 916, 1274, 1381
LweI	GCATC	5 cut(s)	152, 220, 343, 665, 967
MaeI	CTAG	1 cut(s)	1421
MaeII	ACGT	4 cut(s)	269, 418, 544, 1188
MaeIII	GTNAC	4 cut(s)	318, 830, 1135, 1189
MalI	GATC	5 cut(s)	225, 706, 711, 1092, 1295
MbiI	CCGCTC	1 cut(s)	1480
MboI	GATC	5 cut(s)	223, 704, 709, 1090, 1293
MboII	GAAGA	7 cut(s)	314, 394, 397, 625, 650, 933, 1238
MflI	RGATCY	2 cut(s)	704, 1090
MhlI	GDGCHC	4 cut(s)	197, 330, 876, 1527
MlyI	GAGTC	3 cut(s)	1143, 1211, 1433
MmeI	TCCRAC	2 cut(s)	297, 1382
Mph1103I	ATGCAT	1 cut(s)	358
MroXI	GAANNNNTTC	1 cut(s)	106
MseI	TTAA	2 cut(s)	741, 893
MspA1I	CMGCKG	1 cut(s)	1265
MspR9I	CCNGG	2 cut(s)	379, 1017
Mva1269I	GAATGC	2 cut(s)	809, 960
MvaI	CCWGG	2 cut(s)	379, 1017
MvnI	CGCG	1 cut(s)	1281
MwoI	GCNNNNNNNGC	6 cut(s)	353, 1268, 1385, 1394, 1477, 1512
NdeI	CATATG	1 cut(s)	511
NdeII	GATC	5 cut(s)	223, 704, 709, 1090, 1293
NlaIII	CATG	4 cut(s)	25, 851, 1070, 1431
NlaIV	GGNNCC	1 cut(s)	6
NmeAIII	GCCGAG	1 cut(s)	1364
NmuCI	GTSAC	3 cut(s)	318, 830, 1135
NsiI	ATGCAT	1 cut(s)	358
NspI	RCATGY	2 cut(s)	851, 1070
PauI	GCGCGC	1 cut(s)	1279
PciI	ACATGT	1 cut(s)	1066
PcsI	WCGNNNNNNNCGW	1 cut(s)	168
PctI	GAATGC	2 cut(s)	809, 960
PdmI	GAANNNNTTC	1 cut(s)	106
PfeI	GAWTC	2 cut(s)	456, 534
PflMI	CCANNNNNTGG	1 cut(s)	1211
PkrI	GCNGC	5 cut(s)	437, 931, 1264, 1351, 1396
PleI	GAGTC	3 cut(s)	1142, 1211, 1432
PpsI	GAGTC	3 cut(s)	1142, 1211, 1432
PscI	ACATGT	1 cut(s)	1066
PshAI	GACNNNNGTC	1 cut(s)	1174
Psp6I	CCWGG	2 cut(s)	377, 1015
PspGI	CCWGG	2 cut(s)	377, 1015
PspN4I	GGNNCC	1 cut(s)	6
PstNI	CAGNNNCTG	2 cut(s)	1376, 1394
PsuI	RGATCY	2 cut(s)	704, 1090
PteI	GCGCGC	1 cut(s)	1279
PvuII	CAGCTG	1 cut(s)	1265
RsaI	GTAC	5 cut(s)	214, 367, 547, 821, 969
RsaNI	GTAC	5 cut(s)	213, 366, 546, 820, 968
SalI	GTCGAC	1 cut(s)	173
SaqAI	TTAA	2 cut(s)	741, 893
SatI	GCNGC	5 cut(s)	436, 930, 1263, 1350, 1395
Sau3AI	GATC	5 cut(s)	223, 704, 709, 1090, 1293
ScaI	AGTACT	1 cut(s)	214
SchI	GAGTC	3 cut(s)	1143, 1211, 1433
ScrFI	CCNGG	2 cut(s)	379, 1017
SduI	GDGCHC	4 cut(s)	197, 330, 876, 1527
SfaNI	GCATC	5 cut(s)	152, 220, 343, 665, 967
SmlI	CTYRAG	2 cut(s)	1117, 1219
SmoI	CTYRAG	2 cut(s)	1117, 1219
SsiI	CCGC	5 cut(s)	595, 770, 1040, 1350, 1480
SspI	AATATT	1 cut(s)	245
SspMI	CTAG	1 cut(s)	1421
StyD4I	CCNGG	2 cut(s)	377, 1015
TaaI	ACNGT	6 cut(s)	212, 871, 1141, 1165, 1176, 1209
TaiI	ACGT	4 cut(s)	272, 421, 547, 1191
TaqI	TCGA	2 cut(s)	174, 537
TatI	WGTACW	2 cut(s)	212, 365
TauI	GCSGC	1 cut(s)	1352
TfiI	GAWTC	2 cut(s)	456, 534
Tru1I	TTAA	2 cut(s)	741, 893
Tru9I	TTAA	2 cut(s)	741, 893
TscAI	CASTG	7 cut(s)	197, 283, 407, 796, 876, 1144, 1168
TseFI	GTSAC	3 cut(s)	318, 830, 1135
TseI	GCWGC	4 cut(s)	435, 929, 1262, 1394
Tsp45I	GTSAC	3 cut(s)	318, 830, 1135
TspDTI	ATGAA	4 cut(s)	255, 570, 626, 1442
TspGWI	ACGGA	1 cut(s)	251
TspRI	CASTG	7 cut(s)	197, 283, 407, 796, 876, 1144, 1168
Van91I	CCANNNNNTGG	1 cut(s)	1211
VneI	GTGCAC	2 cut(s)	193, 872
XapI	RAATTY	3 cut(s)	557, 952, 1447
XbaI	TCTAGA	1 cut(s)	1420
XceI	RCATGY	2 cut(s)	851, 1070
XmiI	GTMKAC	1 cut(s)	174
XmnI	GAANNNNTTC	1 cut(s)	106
XspI	CTAG	1 cut(s)	1421
ZrmI	AGTACT	1 cut(s)	214
Zsp2I	ATGCAT	1 cut(s)	358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.