FvH4_5g30950

Receptor homology region, transmembrane domain- and RING domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008068
Physical Location & Seq
Forward (+)
21886714 .. 21892522
5809 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30950.t4

Sequence Viewer

Length: 1335 bp
ATGGGCACAATGAATCTGGTATTGGTTTTGATGGTGCTTCTATGTGGGAGAATTTGTATGGCGGATGCCAATGTGGTCTTGATTGGGGACAATGTCACCCTCTCGTTCGAGGACATTGAAGCTAGTTTTGCTCCGGCAGTTAGGGGTTCTGGGGAGTGTGGAGTGTTGTATTTGGCTGAGCCAATTGATGCATGCTCCACATTGACTAATGAAGCCGGACCCGGCACGAATTACAGCTCCCCTTTTGTGTTGATTATAAGAGGGGGGTGTAGTTTTGAGGAGAAAATTAGAAGAGCTCAAAAGGCTATGTTCAAAGCTGCAATTGTGTATGATAACGAAGAAGACGGCATCTTGGTTTCAAGGAGTTCAGCTGGTATAAAAATACATGCAGTTTTTGTTTCAAAAGCTTCTGGTGAAATACTCAAAAAGTACACTGGAGTCTCTAACGTGGAGGTGTGGTTAATTGCAAGCTTTGAGAACTCAGCATGGTCAATCATGGCAATCTCCTTCATTTCCTTGCTCGCCATGTCTGCAGTATTGGCTACTTGCTTTTTTGTTCGCAGACATCGCATAAGACGAGAAAGGCCTCAAGCTTCTCGGGTCCGAGAATTTCATGGGATGAGCAGTAGATTAGTAAGAGCAATGCCAAGTTTGATTTTTACTGCCGTTTTAGAGGACAACTGTACTTCAGTAACATGTGCAATATGCCTCGAGGACTACAGTGTTGGAGAGAAACTTAGGATTTTGCCATGTCGTCATAAATTTCATGCTTTCTGTGTGGACTCTTGGCTTACTTCATGGAGAACCTTTTGTCCAGTTTGCAAGCGTGATGCAAGAGCCAACACAAGTGGTCCACCAGCTTCAGAATCGACACCATTGCTTTCAGCCGGCCCATCCTCTGTGGCTTCCTCTGTACTGTCATCTATGAGATCATCACTGGCATCGTCTTCAGCCATCCAGATAGCACCAGCATCATCAAGGTCTCCTTCTGTTTCTCATCATCACTCTAATTCTAGCACAACATACATCCAGCAGTCTCTTAGGTCCTCCTACCGCCAATCCCCTTCTATGAGTGTAAGCCGAAGCTCAGTAGACCTGCGGAATTCCTCATCCCAAAGGTCTCATGCTTCCTATTATATTTCGCCCCACTCTTCTTACTACCCATCTTTGTCACCTTTGAACTCAAGATATCTGTCTGTACCTGTTCCGAGCCCAAGTAATGCTTCACCAAACTTCATGAGTTCTTCCAGTCATCAACAGCATCCGCTACATTGTAGTGAGTCAGCTGCAACTTTTTCTCCCTTTGCTTCTGCTCATTCTCTTCCTGAATGCTGA

Protein Analysis

445

Amino Acids

48.1

Weight (kDa)

8.79

Isoelectric Point (pI)

70.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 130 - 173 1.7e-12 Ring finger domain
zf-RING_11 PF17123 131 - 158 5.7e-10 RING-like zinc finger
zf-C3HC4 PF00097 131 - 172 3.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 131 - 172 7.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
AbsI CCTCGAGG 1 cut(s) 710
Acc36I ACCTGC 1 cut(s) 1104
AccI GTMKAC 1 cut(s) 1092
AciI CCGC 4 cut(s) 62, 1054, 1099, 1265
AcsI RAATTY 4 cut(s) 51, 608, 761, 1102
AcuI CTGAAG 3 cut(s) 672, 846, 933
AfaI GTAC 4 cut(s) 431, 685, 915, 1200
AflIII ACRYGT 1 cut(s) 695
AgsI TTSAA 5 cut(s) 119, 313, 360, 402, 1180
AjuI GAANNNNNNNTTGG 3 cut(s) 37, 1207, 1239
AloI GAACNNNNNNTCC 2 cut(s) 796, 828
Alw21I GWGCWC 1 cut(s) 298
Alw26I GTCTC 4 cut(s) 445, 987, 1041, 1125
Ama87I CYCGRG 2 cut(s) 597, 710
AoxI GGCC 2 cut(s) 584, 889
ApeKI GCWGC 2 cut(s) 317, 1286
ApoI RAATTY 4 cut(s) 51, 608, 761, 1102
AspS9I GGNCC 5 cut(s) 218, 601, 851, 890, 1044
AsuC2I CCSGG 1 cut(s) 222
AsuHPI GGTGA 4 cut(s) 88, 425, 1164, 1218
AvaI CYCGRG 2 cut(s) 597, 710
AvaII GGWCC 4 cut(s) 218, 601, 851, 1044
BaeGI GKGCMC 1 cut(s) 8
BanII GRGCYC 2 cut(s) 298, 1214
BbsI GAAGAC 2 cut(s) 348, 939
Bbv12I GWGCWC 1 cut(s) 298
BbvI GCAGC 2 cut(s) 304, 1273
BccI CCATC 4 cut(s) 25, 901, 962, 1171
BceAI ACGGC 2 cut(s) 361, 650
BcgI CGANNNNNNTGC 2 cut(s) 859, 893
BcnI CCSGG 1 cut(s) 222
BcoDI GTCTC 4 cut(s) 445, 987, 1041, 1125
BfaI CTAG 2 cut(s) 123, 1014
BfmI CTRYAG 2 cut(s) 531, 718
BfuAI ACCTGC 1 cut(s) 1104
BisI GCNGC 2 cut(s) 318, 1287
BlpI GCTNAGC 1 cut(s) 177
BlsI GCNGC 2 cut(s) 319, 1288
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 4 cut(s) 218, 601, 851, 1044
BmeT110I CYCGRG 2 cut(s) 597, 710
BmgT120I GGNCC 5 cut(s) 218, 601, 851, 890, 1044
BmiI GGNNCC 2 cut(s) 220, 602
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 7 cut(s) 55, 178, 357, 820, 950, 980, 1270
BpiI GAAGAC 2 cut(s) 348, 939
BpmI CTGGAG 1 cut(s) 456
Bpu1102I GCTNAGC 1 cut(s) 177
BpuEI CTTGAG 2 cut(s) 573, 1168
BpuMI CCSGG 1 cut(s) 222
BsaI GGTCTC 2 cut(s) 987, 1125
BsaXI ACNNNNNCTCC 6 cut(s) 40, 70, 443, 473, 1282, 1312
Bse118I RCCGGY 1 cut(s) 887
Bse1I ACTGG 4 cut(s) 439, 815, 942, 1248
Bse3DI GCAATG 2 cut(s) 648, 875
BseGI GGATG 7 cut(s) 70, 624, 893, 954, 1026, 1109, 1261
BseMI GCAATG 2 cut(s) 648, 875
BseMII CTCAG 3 cut(s) 168, 495, 1101
BseNI ACTGG 4 cut(s) 439, 815, 942, 1248
BseRI GAGGAG 1 cut(s) 293
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 2 cut(s) 304, 1273
BshFI GGCC 2 cut(s) 586, 891
BsiHKAI GWGCWC 1 cut(s) 298
BsiHKCI CYCGRG 2 cut(s) 597, 710
BsiSI CCGG 4 cut(s) 134, 216, 222, 888
BslFI GGGAC 1 cut(s) 101
BsmAI GTCTC 4 cut(s) 445, 987, 1041, 1125
BsmFI GGGAC 1 cut(s) 101
BsmI GAATGC 1 cut(s) 1334
BsnI GGCC 2 cut(s) 586, 891
Bso31I GGTCTC 2 cut(s) 987, 1125
BsoBI CYCGRG 2 cut(s) 597, 710
Bsp1286I GDGCHC 3 cut(s) 8, 298, 1214
Bsp143I GATC 1 cut(s) 929
Bsp1720I GCTNAGC 1 cut(s) 177
BspACI CCGC 4 cut(s) 62, 1054, 1099, 1265
BspANI GGCC 2 cut(s) 586, 891
BspCNI CTCAG 3 cut(s) 169, 494, 1100
BspHI TCATGA 1 cut(s) 1236
BspLI GGNNCC 2 cut(s) 220, 602
BspMAI CTGCAG 1 cut(s) 535
BspMI ACCTGC 1 cut(s) 1104
BspQI GCTCTTC 1 cut(s) 286
BspTNI GGTCTC 2 cut(s) 987, 1125
BsrDI GCAATG 2 cut(s) 648, 875
BsrFI RCCGGY 1 cut(s) 887
BsrI ACTGG 4 cut(s) 439, 815, 942, 1248
BssAI RCCGGY 1 cut(s) 887
BssMI GATC 1 cut(s) 929
Bst4CI ACNGT 3 cut(s) 683, 722, 918
Bst6I CTCTTC 3 cut(s) 286, 1156, 1326
BstC8I GCNNGC 5 cut(s) 193, 469, 522, 824, 889
BstDEI CTNAG 5 cut(s) 177, 481, 737, 1040, 1087
BstF5I GGATG 7 cut(s) 70, 624, 893, 954, 1026, 1109, 1261
BstKTI GATC 1 cut(s) 932
BstMAI GTCTC 4 cut(s) 445, 987, 1041, 1125
BstMBI GATC 1 cut(s) 929
BstMWI GCNNNNNNNGC 5 cut(s) 128, 302, 530, 539, 567
BstNSI RCATGY 3 cut(s) 195, 389, 699
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 2 cut(s) 531, 718
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 2 cut(s) 304, 1273
BstV2I GAAGAC 2 cut(s) 348, 939
BsuRI GGCC 2 cut(s) 586, 891
BtgZI GCGATG 1 cut(s) 551
BtsCI GGATG 7 cut(s) 70, 624, 893, 954, 1026, 1109, 1261
BtsIMutI CAGTG 3 cut(s) 432, 727, 935
BveI ACCTGC 1 cut(s) 1104
Cac8I GCNNGC 5 cut(s) 193, 469, 522, 824, 889
CciI TCATGA 1 cut(s) 1236
Cfr10I RCCGGY 1 cut(s) 887
Cfr13I GGNCC 5 cut(s) 218, 601, 851, 890, 1044
Csp6I GTAC 4 cut(s) 430, 684, 914, 1199
CspCI CAANNNNNGTGG 2 cut(s) 829, 864
CviQI GTAC 4 cut(s) 430, 684, 914, 1199
DdeI CTNAG 5 cut(s) 177, 481, 737, 1040, 1087
DpnI GATC 1 cut(s) 931
DpnII GATC 1 cut(s) 929
Eam1104I CTCTTC 3 cut(s) 286, 1156, 1326
EarI CTCTTC 3 cut(s) 286, 1156, 1326
EciI GGCGGA 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 296
Eco147I AGGCCT 1 cut(s) 586
Eco24I GRGCYC 2 cut(s) 298, 1214
Eco31I GGTCTC 2 cut(s) 987, 1125
Eco32I GATATC 1 cut(s) 1190
Eco47I GGWCC 4 cut(s) 218, 601, 851, 1044
Eco53kI GAGCTC 1 cut(s) 296
Eco57I CTGAAG 3 cut(s) 672, 846, 933
Eco88I CYCGRG 2 cut(s) 597, 710
EcoICRI GAGCTC 1 cut(s) 296
EcoO109I RGGNCCY 1 cut(s) 1044
EcoRI GAATTC 1 cut(s) 1102
EcoRV GATATC 1 cut(s) 1190
EcoT22I ATGCAT 1 cut(s) 193
EcoT38I GRGCYC 2 cut(s) 298, 1214
FalI AAGNNNNNCTT 4 cut(s) 970, 1002, 1207, 1239
FaqI GGGAC 1 cut(s) 101
FblI GTMKAC 1 cut(s) 1092
Fnu4HI GCNGC 2 cut(s) 318, 1287
FokI GGATG 7 cut(s) 77, 631, 880, 941, 1013, 1096, 1248
FriOI GRGCYC 2 cut(s) 298, 1214
Fsp4HI GCNGC 2 cut(s) 318, 1287
FspBI CTAG 2 cut(s) 123, 1014
GluI GCNGC 2 cut(s) 318, 1287
GsuI CTGGAG 1 cut(s) 456
HaeIII GGCC 2 cut(s) 586, 891
HapII CCGG 4 cut(s) 134, 216, 222, 888
HindIII AAGCTT 3 cut(s) 405, 469, 591
HinfI GANTC 5 cut(s) 13, 438, 782, 866, 1280
HpaII CCGG 4 cut(s) 134, 216, 222, 888
HphI GGTGA 4 cut(s) 88, 425, 1164, 1218
Hpy166II GTNNAC 4 cut(s) 432, 781, 854, 1093
Hpy188I TCNGA 3 cut(s) 605, 865, 1209
Hpy188III TCNNGA 5 cut(s) 79, 958, 1185, 1237, 1325
Hpy8I GTNNAC 4 cut(s) 432, 781, 854, 1093
HpyAV CCTTC 3 cut(s) 517, 996, 1074
HpyCH4III ACNGT 3 cut(s) 683, 722, 918
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 9 cut(s) 191, 320, 389, 467, 533, 701, 822, 833, 1289
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 302, 530, 539, 567
HpyF3I CTNAG 5 cut(s) 177, 481, 737, 1040, 1087
HpySE526I ACGT 1 cut(s) 447
KroI GCCGGC 1 cut(s) 887
KroNI GCCGGC 1 cut(s) 889
Kzo9I GATC 1 cut(s) 929
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 3 cut(s) 136, 200, 242
Lsp1109I GCAGC 2 cut(s) 304, 1273
LweI GCATC 7 cut(s) 55, 178, 357, 820, 950, 980, 1270
MaeI CTAG 2 cut(s) 123, 1014
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 3 cut(s) 94, 691, 1170
MalI GATC 1 cut(s) 931
MboI GATC 1 cut(s) 929
MboII GAAGA 7 cut(s) 303, 350, 353, 939, 1143, 1236, 1313
MfeI CAATTG 2 cut(s) 183, 321
MhlI GDGCHC 3 cut(s) 8, 298, 1214
MlyI GAGTC 3 cut(s) 447, 776, 1289
MmeI TCCRAC 1 cut(s) 706
Mph1103I ATGCAT 1 cut(s) 193
MroNI GCCGGC 1 cut(s) 887
MseI TTAA 1 cut(s) 461
MslI CAYNNNNRTG 1 cut(s) 1275
MspA1I CMGCKG 2 cut(s) 371, 1286
MspI CCGG 4 cut(s) 134, 216, 222, 888
MspR9I CCNGG 1 cut(s) 222
MunI CAATTG 2 cut(s) 183, 321
Mva1269I GAATGC 1 cut(s) 1334
MwoI GCNNNNNNNGC 5 cut(s) 128, 302, 530, 539, 567
NaeI GCCGGC 1 cut(s) 889
NciI CCSGG 1 cut(s) 222
NdeII GATC 1 cut(s) 929
NgoMIV GCCGGC 1 cut(s) 887
NlaIV GGNNCC 2 cut(s) 220, 602
NmuCI GTSAC 2 cut(s) 94, 1170
NsiI ATGCAT 1 cut(s) 193
NspI RCATGY 3 cut(s) 195, 389, 699
PaeI GCATGC 1 cut(s) 195
PaeR7I CTCGAG 1 cut(s) 710
PagI TCATGA 1 cut(s) 1236
PceI AGGCCT 1 cut(s) 586
PciI ACATGT 1 cut(s) 695
PciSI GCTCTTC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PctI GAATGC 1 cut(s) 1334
PdiI GCCGGC 1 cut(s) 889
PfeI GAWTC 2 cut(s) 13, 866
PflFI GACNNNGTC 1 cut(s) 92
PkrI GCNGC 2 cut(s) 319, 1288
PleI GAGTC 3 cut(s) 446, 776, 1288
PpsI GAGTC 3 cut(s) 446, 776, 1288
PpuMI RGGWCCY 1 cut(s) 1044
PscI ACATGT 1 cut(s) 695
PsiI TTATAA 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 1044
PspN4I GGNNCC 2 cut(s) 220, 602
PspPI GGNCC 5 cut(s) 218, 601, 851, 890, 1044
PspPPI RGGWCCY 1 cut(s) 1044
PspXI VCTCGAGB 1 cut(s) 710
PstI CTGCAG 1 cut(s) 535
PsyI GACNNNGTC 1 cut(s) 92
PvuII CAGCTG 2 cut(s) 371, 1286
RsaI GTAC 4 cut(s) 431, 685, 915, 1200
RsaNI GTAC 4 cut(s) 430, 684, 914, 1199
RseI CAYNNNNRTG 1 cut(s) 1275
SacI GAGCTC 1 cut(s) 298
SapI GCTCTTC 1 cut(s) 286
SaqAI TTAA 1 cut(s) 461
SatI GCNGC 2 cut(s) 318, 1287
Sau3AI GATC 1 cut(s) 929
Sau96I GGNCC 5 cut(s) 218, 601, 851, 890, 1044
SchI GAGTC 3 cut(s) 447, 776, 1289
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 3 cut(s) 8, 298, 1214
SfaNI GCATC 7 cut(s) 55, 178, 357, 820, 950, 980, 1270
SfcI CTRYAG 2 cut(s) 531, 718
Sfr274I CTCGAG 1 cut(s) 710
SinI GGWCC 4 cut(s) 218, 601, 851, 1044
SlaI CTCGAG 1 cut(s) 710
SmiMI CAYNNNNRTG 1 cut(s) 1275
SmlI CTYRAG 3 cut(s) 588, 710, 1183
SmoI CTYRAG 3 cut(s) 588, 710, 1183
SphI GCATGC 1 cut(s) 195
SseBI AGGCCT 1 cut(s) 586
SsiI CCGC 4 cut(s) 62, 1054, 1099, 1265
SspMI CTAG 2 cut(s) 123, 1014
SstI GAGCTC 1 cut(s) 298
StuI AGGCCT 1 cut(s) 586
StyD4I CCNGG 1 cut(s) 220
TaaI ACNGT 3 cut(s) 683, 722, 918
TaiI ACGT 1 cut(s) 450
TaqI TCGA 3 cut(s) 108, 711, 869
TatI WGTACW 3 cut(s) 429, 683, 913
TfiI GAWTC 2 cut(s) 13, 866
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TscAI CASTG 3 cut(s) 439, 727, 942
TseFI GTSAC 2 cut(s) 94, 1170
TseI GCWGC 2 cut(s) 317, 1286
Tsp45I GTSAC 2 cut(s) 94, 1170
TspDTI ATGAA 7 cut(s) 26, 225, 499, 602, 755, 786, 1225
TspRI CASTG 3 cut(s) 439, 727, 942
Tth111I GACNNNGTC 1 cut(s) 92
VpaK11BI GGWCC 4 cut(s) 218, 601, 851, 1044
XapI RAATTY 4 cut(s) 51, 608, 761, 1102
XceI RCATGY 3 cut(s) 195, 389, 699
XhoI CTCGAG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 1092
XspI CTAG 2 cut(s) 123, 1014
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.