Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0008068

Physical Location & Seq

Genomic Coordinates Reverse (-)

18849602 .. 18855743

Gene Structure 6142 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00233040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGGGCACAATGAATCTGGTCGTGGTTTTGATGGCGCTTCTCTTTGGGAAGTTTTGCATGGCGGATGCCAATGTGGTCTTGATTGGGAAAAACGTCACCCTCTCTTTCGAGGACGTTGAAGCTAATTTTGCTCCGGCAGTTAGGGGTTCTGGGGAGTGTGGAGTGTTGTATTTGGCTGAGCCGATTGATGCATGCTCGACATTGACTAATAAAGCCGGACCGGGCACGAATTATAGCTCCCCTTTTGTGTTGATTATAAGAGGAGGGGGTTGCAGTTTTGAGGAGAAAGTTAGAAGAGCTCAAGAGGCTGGGTTCAAAGCTGCAATTGTCTATGACAATGAAGACGACGGCGTCTTGGTTTCAATGGCAGGAAGTTCAGCTGGTATAAAAATACATGCAGTTTTTGTTTCAAAAGCTTCTGGTGAAATTCTCAAAATGTACACTGGACTCACTAACATGGAGGTGTGGCTAATTGCAAGCTTTGAGAACTCAGCATGGTCAATCATGGCAATCTCCTTCATTTCCTTGCTCGCCATGTCTGCAGTATTGGCTACTTGCTTTTTCGTTCGCAGACATCGCATAAGACGAGAAAGGCCTCAAGCTTCTCGGGTCCGAGAATTTCATGGGATGAGCGGTAGATTAGTAAAAGCAATGCCAAGTTTGATATTTACTGCCGTTTTAGAGGACAACTGCACTTCAGTAACATGTGCAATATGCCTTGAGGACTACAGTGTTGGAGAGAAACTTAGGATTCTGCCGTGTTGTCACACATATCTCAATGTAATAACTTTGGGTGTTGCAGAGTTTCATGCTGTCTGTGTGGACTCTTGGCTTACTTCATGGAGAACCTTTTGCCCAGTTTGCAAGCGTGATGCAAGAGCCAACATGAGTGATCTACCAGCTTCAGAATCGACACCATTGCTTTCACCCGGCCCATCCTCTGTGGCCTCCTCTGTACTGTCGTCTAGGAGATCATCACTAGCATCATCTTCAGCCATTCAGATAGTACCAGAATCATCAAGGTCTCCTTATGTTTCTCATCCTCACTCTCTTTCTAGCACATACATCCAGCAGTCTCTTAGGTCATCCTTCCGCCAGTCCCCTTCCATGAGTGTAAGCCGAAGCTCAGTAGACCTGCGGAATTCCTCATCCCAAAGGTCTCATGCTTCCTATTTTATTTCTCCCCACTCCTCGTACTACCCATCTTTATCACCACTGAACTCGAGATATCTGACTGTACCCATTCCAAGCGCAAGCAATGCTTCACCAAGTTTCTTGAGTTCTTCCAGTCATCAACAGCATCCACTACATTGTAGTGAGTCAGCAGCAACCTTTTCTCCTTTTGCTTCTGCTCATTCTCTTCCGGAATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000209 GO:0000322 GO:0000325 GO:0000326 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0031090 GO:0031984 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0070647 GO:0071704 GO:0098588 GO:0098791 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA zf-RING_2

Protein Analysis

457

Amino Acids

49.33

Weight (kDa)

8.33

Isoelectric Point (pI)

67.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	62 - 144	1.3e-12	PA domain
zf-RING_2	PF13639	235 - 289	4.1e-08	Ring finger domain
zf-RING_11	PF17123	236 - 258	3.6e-07	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71980
fragaria_vesca	FvH4_5g30950 FvH4_5g30950 FvH4_5g30950 FvH4_5g30950 FvH4_5g30950
malus_domestica	MD08G1219500.v1.1 MD15G1408100.v1.1
prunus_persica	Prupe.1G553100_v2.0.a1
pyrus_communis	pycom08g19050 pycom15g36420 pycom15g36430
rosa_chinensis	RchiOBHm_Chr7g0226311
rosa_laevigata	RLG00000001752
rosa_multiflora	Rmu_sc0004322.1_g000023
rosa_roxburghii	Rroxscaffold_3G00233040
rosa_rugosa	Rorug07G0236300 Rorug07G0236400 Rorug07G0236500
rosa_samantha	Rh7AG383500 Rh7BG370300 Rh7CG403100 Rh7DG381900
rosa_wichuraiana	Rw7G032120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	257
AasI	GACNNNNNNGTC	1 cut(s)	350
Acc36I	ACCTGC	1 cut(s)	1143
AccBSI	CCGCTC	1 cut(s)	633
AccI	GTMKAC	1 cut(s)	1131
AccIII	TCCGGA	1 cut(s)	1363
AciI	CCGC	4 cut(s)	62, 633, 1093, 1138
AcsI	RAATTY	3 cut(s)	426, 617, 1141
AcuI	CTGAAG	3 cut(s)	681, 888, 975
AcyI	GRCGYC	1 cut(s)	351
AfaI	GTAC	5 cut(s)	440, 957, 1008, 1196, 1239
AflIII	ACRYGT	1 cut(s)	704
AgsI	TTSAA	4 cut(s)	119, 316, 363, 411
Alw21I	GWGCWC	1 cut(s)	301
Alw26I	GTCTC	3 cut(s)	1029, 1080, 1164
Ama87I	CYCGRG	2 cut(s)	606, 1222
Aor13HI	TCCGGA	1 cut(s)	1363
AoxI	GGCC	3 cut(s)	593, 931, 945
ApeKI	GCWGC	2 cut(s)	320, 1325
ApoI	RAATTY	3 cut(s)	426, 617, 1141
AspLEI	GCGC	2 cut(s)	37, 1253
AspS9I	GGNCC	3 cut(s)	218, 610, 932
AsuC2I	CCSGG	2 cut(s)	222, 930
AsuHPI	GGTGA	5 cut(s)	88, 434, 918, 1203, 1257
AvaI	CYCGRG	2 cut(s)	606, 1222
AvaII	GGWCC	2 cut(s)	218, 610
BaeGI	GKGCMC	2 cut(s)	8, 227
BanII	GRGCYC	1 cut(s)	301
BbsI	GAAGAC	1 cut(s)	348
Bbv12I	GWGCWC	1 cut(s)	301
BbvI	GCAGC	2 cut(s)	307, 1337
BccI	CCATC	3 cut(s)	25, 943, 1210
BceAI	ACGGC	3 cut(s)	364, 659, 742
BcgI	CGANNNNNNTGC	2 cut(s)	901, 935
BcnI	CCSGG	2 cut(s)	222, 930
BcoDI	GTCTC	3 cut(s)	1029, 1080, 1164
BfaI	CTAG	3 cut(s)	966, 980, 1056
BfmI	CTRYAG	2 cut(s)	540, 727
BfoI	RGCGCY	1 cut(s)	38
BfuAI	ACCTGC	1 cut(s)	1143
BisI	GCNGC	2 cut(s)	321, 1326
BlpI	GCTNAGC	1 cut(s)	177
BlsI	GCNGC	2 cut(s)	322, 1327
Bme1390I	CCNGG	2 cut(s)	222, 930
Bme18I	GGWCC	2 cut(s)	218, 610
BmeT110I	CYCGRG	2 cut(s)	606, 1222
BmgT120I	GGNCC	3 cut(s)	218, 610, 932
BmiI	GGNNCC	1 cut(s)	611
BmrFI	CCNGG	2 cut(s)	222, 930
BmrI	ACTGGG	1 cut(s)	851
BmsI	GCATC	5 cut(s)	55, 178, 862, 992, 1309
BmuI	ACTGGG	1 cut(s)	851
BpiI	GAAGAC	1 cut(s)	348
Bpu1102I	GCTNAGC	1 cut(s)	177
BpuEI	CTTGAG	4 cut(s)	285, 582, 740, 1297
BpuMI	CCSGG	2 cut(s)	222, 930
BsaHI	GRCGYC	1 cut(s)	351
BsaI	GGTCTC	2 cut(s)	1029, 1164
BsaWI	WCCGGW	1 cut(s)	1363
BsaXI	ACNNNNNCTCC	2 cut(s)	1321, 1351
Bse1I	ACTGG	4 cut(s)	448, 857, 1096, 1287
Bse3DI	GCAATG	3 cut(s)	657, 917, 1264
BseAI	TCCGGA	1 cut(s)	1363
BseGI	GGATG	8 cut(s)	70, 633, 935, 1039, 1065, 1085, 1148, 1300
BseMI	GCAATG	3 cut(s)	657, 917, 1264
BseMII	CTCAG	3 cut(s)	168, 504, 1140
BseNI	ACTGG	4 cut(s)	448, 857, 1096, 1287
BseRI	GAGGAG	4 cut(s)	276, 296, 940, 1180
BseSI	GKGCMC	2 cut(s)	8, 227
BseXI	GCAGC	2 cut(s)	307, 1337
BseYI	CCCAGC	1 cut(s)	308
BsgI	GTGCAG	1 cut(s)	676
BshFI	GGCC	3 cut(s)	595, 933, 947
BsiHKAI	GWGCWC	1 cut(s)	301
BsiHKCI	CYCGRG	2 cut(s)	606, 1222
BsiSI	CCGG	5 cut(s)	134, 216, 221, 930, 1364
BslFI	GGGAC	1 cut(s)	1084
BsmAI	GTCTC	3 cut(s)	1029, 1080, 1164
BsmFI	GGGAC	1 cut(s)	1084
BsmI	GAATGC	1 cut(s)	1373
BsnI	GGCC	3 cut(s)	595, 933, 947
Bso31I	GGTCTC	2 cut(s)	1029, 1164
BsoBI	CYCGRG	2 cut(s)	606, 1222
Bsp1286I	GDGCHC	3 cut(s)	8, 227, 301
Bsp13I	TCCGGA	1 cut(s)	1363
Bsp1407I	TGTACA	1 cut(s)	438
Bsp143I	GATC	2 cut(s)	892, 971
Bsp1720I	GCTNAGC	1 cut(s)	177
BspACI	CCGC	4 cut(s)	62, 633, 1093, 1138
BspANI	GGCC	3 cut(s)	595, 933, 947
BspCNI	CTCAG	3 cut(s)	169, 503, 1139
BspEI	TCCGGA	1 cut(s)	1363
BspLI	GGNNCC	1 cut(s)	611
BspMAI	CTGCAG	1 cut(s)	544
BspMI	ACCTGC	1 cut(s)	1143
BspQI	GCTCTTC	1 cut(s)	289
BspTNI	GGTCTC	2 cut(s)	1029, 1164
BsrBI	CCGCTC	1 cut(s)	633
BsrDI	GCAATG	3 cut(s)	657, 917, 1264
BsrGI	TGTACA	1 cut(s)	438
BsrI	ACTGG	4 cut(s)	448, 857, 1096, 1287
BssMI	GATC	2 cut(s)	892, 971
BssNI	GRCGYC	1 cut(s)	351
Bst4CI	ACNGT	3 cut(s)	731, 960, 1237
Bst6I	CTCTTC	2 cut(s)	289, 1365
BstACI	GRCGYC	1 cut(s)	351
BstAPI	GCANNNNNTGC	1 cut(s)	1259
BstAUI	TGTACA	1 cut(s)	438
BstC8I	GCNNGC	5 cut(s)	193, 478, 531, 866, 1255
BstDEI	CTNAG	5 cut(s)	177, 490, 746, 1079, 1126
BstF5I	GGATG	8 cut(s)	70, 633, 935, 1039, 1065, 1085, 1148, 1300
BstH2I	RGCGCY	1 cut(s)	38
BstHHI	GCGC	2 cut(s)	37, 1253
BstKTI	GATC	2 cut(s)	895, 974
BstMAI	GTCTC	3 cut(s)	1029, 1080, 1164
BstMBI	GATC	2 cut(s)	892, 971
BstMWI	GCNNNNNNNGC	7 cut(s)	128, 305, 539, 548, 576, 861, 1259
BstNSI	RCATGY	3 cut(s)	195, 398, 708
BstSCI	CCNGG	2 cut(s)	220, 928
BstSFI	CTRYAG	2 cut(s)	540, 727
BstSLI	GKGCMC	2 cut(s)	8, 227
BstV1I	GCAGC	2 cut(s)	307, 1337
BstV2I	GAAGAC	1 cut(s)	348
BsuRI	GGCC	3 cut(s)	595, 933, 947
BtgZI	GCGATG	1 cut(s)	560
BtsCI	GGATG	8 cut(s)	70, 633, 935, 1039, 1065, 1085, 1148, 1300
BtsIMutI	CAGTG	3 cut(s)	441, 736, 1214
BveI	ACCTGC	1 cut(s)	1143
Cac8I	GCNNGC	5 cut(s)	193, 478, 531, 866, 1255
CfoI	GCGC	2 cut(s)	37, 1253
Cfr13I	GGNCC	3 cut(s)	218, 610, 932
CpoI	CGGWCCG	1 cut(s)	218
CseI	GACGC	1 cut(s)	340
Csp6I	GTAC	5 cut(s)	439, 956, 1007, 1195, 1238
CspI	CGGWCCG	1 cut(s)	218
CviQI	GTAC	5 cut(s)	439, 956, 1007, 1195, 1238
DdeI	CTNAG	5 cut(s)	177, 490, 746, 1079, 1126
DpnI	GATC	2 cut(s)	894, 973
DpnII	GATC	2 cut(s)	892, 971
DrdI	GACNNNNNNGTC	1 cut(s)	350
DseDI	GACNNNNNNGTC	1 cut(s)	350
Eam1104I	CTCTTC	2 cut(s)	289, 1365
EarI	CTCTTC	2 cut(s)	289, 1365
EciI	GGCGGA	2 cut(s)	77, 1082
Ecl136II	GAGCTC	1 cut(s)	299
Eco147I	AGGCCT	1 cut(s)	595
Eco24I	GRGCYC	1 cut(s)	301
Eco31I	GGTCTC	2 cut(s)	1029, 1164
Eco32I	GATATC	1 cut(s)	1229
Eco47I	GGWCC	2 cut(s)	218, 610
Eco53kI	GAGCTC	1 cut(s)	299
Eco57I	CTGAAG	3 cut(s)	681, 888, 975
Eco88I	CYCGRG	2 cut(s)	606, 1222
EcoICRI	GAGCTC	1 cut(s)	299
EcoRI	GAATTC	1 cut(s)	1141
EcoRV	GATATC	1 cut(s)	1229
EcoT22I	ATGCAT	1 cut(s)	193
EcoT38I	GRGCYC	1 cut(s)	301
FalI	AAGNNNNNCTT	4 cut(s)	1012, 1044, 1246, 1278
FaqI	GGGAC	1 cut(s)	1084
FblI	GTMKAC	1 cut(s)	1131
Fnu4HI	GCNGC	2 cut(s)	321, 1326
FokI	GGATG	8 cut(s)	77, 640, 922, 1026, 1052, 1072, 1135, 1287
FriOI	GRGCYC	1 cut(s)	301
Fsp4HI	GCNGC	2 cut(s)	321, 1326
FspBI	CTAG	3 cut(s)	966, 980, 1056
GlaI	GCGC	2 cut(s)	36, 1252
GluI	GCNGC	2 cut(s)	321, 1326
GsaI	CCCAGC	1 cut(s)	312
HaeII	RGCGCY	1 cut(s)	38
HaeIII	GGCC	3 cut(s)	595, 933, 947
HapII	CCGG	5 cut(s)	134, 216, 221, 930, 1364
HgaI	GACGC	1 cut(s)	340
HhaI	GCGC	2 cut(s)	37, 1253
Hin1I	GRCGYC	1 cut(s)	351
Hin6I	GCGC	2 cut(s)	35, 1251
HinP1I	GCGC	2 cut(s)	35, 1251
HindIII	AAGCTT	3 cut(s)	414, 478, 600
HinfI	GANTC	7 cut(s)	13, 447, 751, 824, 908, 1013, 1319
HpaII	CCGG	5 cut(s)	134, 216, 221, 930, 1364
HphI	GGTGA	5 cut(s)	88, 434, 918, 1203, 1257
Hpy166II	GTNNAC	3 cut(s)	441, 823, 1132
Hpy188I	TCNGA	4 cut(s)	614, 907, 1002, 1233
Hpy188III	TCNNGA	5 cut(s)	79, 302, 1224, 1276, 1364
Hpy8I	GTNNAC	3 cut(s)	441, 823, 1132
Hpy99I	CGWCG	1 cut(s)	350
HpyAV	CCTTC	3 cut(s)	526, 1099, 1113
HpyCH4III	ACNGT	3 cut(s)	731, 960, 1237
HpyCH4IV	ACGT	2 cut(s)	93, 114
HpyF10VI	GCNNNNNNNGC	7 cut(s)	128, 305, 539, 548, 576, 861, 1259
HpyF3I	CTNAG	5 cut(s)	177, 490, 746, 1079, 1126
HpySE526I	ACGT	2 cut(s)	93, 114
Hsp92I	GRCGYC	1 cut(s)	351
HspAI	GCGC	2 cut(s)	35, 1251
Kpn2I	TCCGGA	1 cut(s)	1363
Kzo9I	GATC	2 cut(s)	892, 971
LguI	GCTCTTC	1 cut(s)	289
LmnI	GCTCC	2 cut(s)	136, 242
Lsp1109I	GCAGC	2 cut(s)	307, 1337
LweI	GCATC	5 cut(s)	55, 178, 862, 992, 1309
MaeI	CTAG	3 cut(s)	966, 980, 1056
MaeII	ACGT	2 cut(s)	93, 114
MaeIII	GTNAC	3 cut(s)	94, 700, 764
MalI	GATC	2 cut(s)	894, 973
MbiI	CCGCTC	1 cut(s)	633
MboI	GATC	2 cut(s)	892, 971
MboII	GAAGA	5 cut(s)	306, 353, 981, 1275, 1352
MfeI	CAATTG	1 cut(s)	324
MhlI	GDGCHC	3 cut(s)	8, 227, 301
MluCI	AATT	7 cut(s)	124, 229, 324, 426, 471, 617, 1141
MlyI	GAGTC	3 cut(s)	441, 818, 1328
MmeI	TCCRAC	1 cut(s)	715
Mph1103I	ATGCAT	1 cut(s)	193
MroI	TCCGGA	1 cut(s)	1363
MslI	CAYNNNNRTG	3 cut(s)	455, 461, 1314
MspA1I	CMGCKG	1 cut(s)	380
MspI	CCGG	5 cut(s)	134, 216, 221, 930, 1364
MspR9I	CCNGG	2 cut(s)	222, 930
MunI	CAATTG	1 cut(s)	324
Mva1269I	GAATGC	1 cut(s)	1373
MwoI	GCNNNNNNNGC	7 cut(s)	128, 305, 539, 548, 576, 861, 1259
NciI	CCSGG	2 cut(s)	222, 930
NdeII	GATC	2 cut(s)	892, 971
NlaIV	GGNNCC	1 cut(s)	611
NmuCI	GTSAC	2 cut(s)	94, 764
NsiI	ATGCAT	1 cut(s)	193
NspI	RCATGY	3 cut(s)	195, 398, 708
PaeI	GCATGC	1 cut(s)	195
PaeR7I	CTCGAG	1 cut(s)	1222
PceI	AGGCCT	1 cut(s)	595
PciI	ACATGT	1 cut(s)	704
PciSI	GCTCTTC	1 cut(s)	289
PcsI	WCGNNNNNNNCGW	1 cut(s)	583
PctI	GAATGC	1 cut(s)	1373
PfeI	GAWTC	4 cut(s)	13, 751, 908, 1013
PflFI	GACNNNGTC	1 cut(s)	350
PkrI	GCNGC	2 cut(s)	322, 1327
PleI	GAGTC	3 cut(s)	441, 818, 1327
PpsI	GAGTC	3 cut(s)	441, 818, 1327
PscI	ACATGT	1 cut(s)	704
PsiI	TTATAA	1 cut(s)	257
Psp124BI	GAGCTC	1 cut(s)	301
PspFI	CCCAGC	1 cut(s)	308
PspN4I	GGNNCC	1 cut(s)	611
PspPI	GGNCC	3 cut(s)	218, 610, 932
PstI	CTGCAG	1 cut(s)	544
PsyI	GACNNNGTC	1 cut(s)	350
PvuII	CAGCTG	1 cut(s)	380
RsaI	GTAC	5 cut(s)	440, 957, 1008, 1196, 1239
RsaNI	GTAC	5 cut(s)	439, 956, 1007, 1195, 1238
RseI	CAYNNNNRTG	3 cut(s)	455, 461, 1314
Rsr2I	CGGWCCG	1 cut(s)	218
RsrII	CGGWCCG	1 cut(s)	218
SacI	GAGCTC	1 cut(s)	301
SapI	GCTCTTC	1 cut(s)	289
SatI	GCNGC	2 cut(s)	321, 1326
Sau3AI	GATC	2 cut(s)	892, 971
Sau96I	GGNCC	3 cut(s)	218, 610, 932
SchI	GAGTC	3 cut(s)	441, 818, 1328
ScrFI	CCNGG	2 cut(s)	222, 930
SduI	GDGCHC	3 cut(s)	8, 227, 301
SfaNI	GCATC	5 cut(s)	55, 178, 862, 992, 1309
SfcI	CTRYAG	2 cut(s)	540, 727
Sfr274I	CTCGAG	1 cut(s)	1222
SinI	GGWCC	2 cut(s)	218, 610
SlaI	CTCGAG	1 cut(s)	1222
SmiMI	CAYNNNNRTG	3 cut(s)	455, 461, 1314
SmlI	CTYRAG	5 cut(s)	300, 597, 719, 1222, 1276
SmoI	CTYRAG	5 cut(s)	300, 597, 719, 1222, 1276
SphI	GCATGC	1 cut(s)	195
Sse9I	AATT	7 cut(s)	124, 229, 324, 426, 471, 617, 1141
SseBI	AGGCCT	1 cut(s)	595
SsiI	CCGC	4 cut(s)	62, 633, 1093, 1138
SspMI	CTAG	3 cut(s)	966, 980, 1056
SstI	GAGCTC	1 cut(s)	301
StuI	AGGCCT	1 cut(s)	595
StyD4I	CCNGG	2 cut(s)	220, 928
TaaI	ACNGT	3 cut(s)	731, 960, 1237
TaiI	ACGT	2 cut(s)	96, 117
TaqI	TCGA	4 cut(s)	108, 197, 911, 1223
TasI	AATT	7 cut(s)	124, 229, 324, 426, 471, 617, 1141
TatI	WGTACW	2 cut(s)	438, 955
TfiI	GAWTC	4 cut(s)	13, 751, 908, 1013
TscAI	CASTG	3 cut(s)	448, 736, 1221
TseFI	GTSAC	2 cut(s)	94, 764
TseI	GCWGC	2 cut(s)	320, 1325
Tsp45I	GTSAC	2 cut(s)	94, 764
TspDTI	ATGAA	6 cut(s)	26, 354, 508, 611, 797, 828
TspRI	CASTG	3 cut(s)	448, 736, 1221
Tth111I	GACNNNGTC	1 cut(s)	350
VpaK11BI	GGWCC	2 cut(s)	218, 610
XapI	RAATTY	3 cut(s)	426, 617, 1141
XceI	RCATGY	3 cut(s)	195, 398, 708
XhoI	CTCGAG	1 cut(s)	1222
XmiI	GTMKAC	1 cut(s)	1131
XspI	CTAG	3 cut(s)	966, 980, 1056
Zsp2I	ATGCAT	1 cut(s)	193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.