Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0008068

Physical Location & Seq

Genomic Coordinates Forward (+)

51910899 .. 51917529

Gene Structure 6631 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG381900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1341 bp

ATGGGCACAATGAATCTGGTCGTGGTTTTGGTGGCGCTTGTCTTTGGGAAGTTTTGCATGGCGGATGCCAATGTGGTCTTGATTGGGAGCAACGTCACCCTCTCTTTCGAGGACGTTGAAGCTAATTTTGCTCCGGCAGTTAGGGGTTCTGGGCAGTGTGGAGTGTTGTATTTGGCTGAGCCGATTGATGCATGCTCGACATTGACTAATAAAGCCGGACCGGGCGCGAATTATAGCTCCCCTTTTGTGTTGATTAGAAGAGGAGGGGGTTGCAGTTTTGAGGAGAAAGTTAGAAGAGCTCAAGATGCTGGGTTCAAAGCTGCAATTGTCTATGACAATGAAGACGACGGCGTCTTGGTTTCAATGGCAGGAAGTTCAGCTGGTATAAAAATACATGCAGTTTTTGTTTCAAAAGCTTCTGGTGAAATTCTCAAAATGTACGCTGGACTCACTAACATGGAGGTGTGGCTAATTGCCAGCTTTGAGAACTCAGCATGGTCAATCATGGCAATCTCCTTCATTTCCTTGCTCGCCATGTCTGCAGTATTGGCTACTTGCTTTTTCGTTCGCAGACATCGCATAAGACGAGAAAGGCCTCAAGCTTCTCGGGTCCGAGAATTTCATGGGATGAGCAGTAGATTAGTAAAAGCAATGCCAAGTTTGATATTTACTGCCGTTTTAGAGGACAACTGCACTTCAGTAACATGTGCAATATGCCTTGAGGACTACAGTGTTGGAGAGAAACTTAGGATTCTGCCTTGTTGTCACAAGTTTCATGCTGTCTGTGTGGACTCTTGGCTTACTTCATGGAGAACCTTTTGCCCAGTTTGCAAGCGTGATGCAAGAGCCAACATGAGTGATCTACCAGCTTCAGAATCGACACCATTGCTTTCACCCGGCCCATCCTCTGTGGCCTCCTCTGTACTGTCATCTAGGAGATCATCACTGGCATCATCTTCAGCCATTCAGATAGTACCAGAATCATCAAGGTCTCCTTATGTTTCTCATCCTCACTCTCTTTCTAGCACATACATCCAGCAGTCTCTTAGGTCCTCCTTCCGCCAGTCCCCTTCTATGAGTGTAAGCCGAAGCTCAGTAGACCTGCGGAATTCCTCATCCCAAAGGTCTCATGCTTCCTATTTTATTTCTCCCCACTCCTCATACTACCCATCTTTATCACCACTGAACTCGAGATATCTGACTGTACCCATTCCAAGCGCAAGCAATGCTTCACCAAGTTTCTTGAGTTCTTCCAGTCATCAACAGCATCCACTACACTGTAGTGAGTCAGCAGCAACCTTTTCTCCTTTTGCTTCTGCTCATTCTCTTCCTGAATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000209 GO:0000322 GO:0000325 GO:0000326 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0031090 GO:0031984 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0070647 GO:0071704 GO:0098588 GO:0098791 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA zf-RING_2

Protein Analysis

446

Amino Acids

48.09

Weight (kDa)

8.7

Isoelectric Point (pI)

72.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	61 - 144	2.7e-13	PA domain
zf-RING_2	PF13639	235 - 278	4.9e-12	Ring finger domain
zf-RING_11	PF17123	236 - 263	2.5e-09	RING-like zinc finger
zf-C3HC4	PF00097	236 - 277	3.5e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71980
fragaria_vesca	FvH4_5g30950 FvH4_5g30950 FvH4_5g30950 FvH4_5g30950 FvH4_5g30950
malus_domestica	MD08G1219500.v1.1 MD15G1408100.v1.1
prunus_persica	Prupe.1G553100_v2.0.a1
pyrus_communis	pycom08g19050 pycom15g36420 pycom15g36430
rosa_chinensis	RchiOBHm_Chr7g0226311
rosa_laevigata	RLG00000001752
rosa_multiflora	Rmu_sc0004322.1_g000023
rosa_roxburghii	Rroxscaffold_3G00233040
rosa_rugosa	Rorug07G0236300 Rorug07G0236400 Rorug07G0236500
rosa_samantha	Rh7AG383500 Rh7BG370300 Rh7CG403100 Rh7DG381900
rosa_wichuraiana	Rw7G032120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	350
Acc36I	ACCTGC	1 cut(s)	1110
AccI	GTMKAC	1 cut(s)	1098
AccII	CGCG	1 cut(s)	227
AciI	CCGC	3 cut(s)	62, 1060, 1105
AcsI	RAATTY	3 cut(s)	426, 617, 1108
AcuI	CTGAAG	3 cut(s)	681, 855, 942
AcyI	GRCGYC	1 cut(s)	351
AfaI	GTAC	4 cut(s)	440, 924, 975, 1206
AflIII	ACRYGT	1 cut(s)	704
AgsI	TTSAA	4 cut(s)	119, 316, 363, 411
AleI	CACNNNNGTG	1 cut(s)	1281
Alw21I	GWGCWC	1 cut(s)	301
Alw26I	GTCTC	3 cut(s)	996, 1047, 1131
Ama87I	CYCGRG	2 cut(s)	606, 1189
AoxI	GGCC	3 cut(s)	593, 898, 912
ApeKI	GCWGC	2 cut(s)	320, 1292
ApoI	RAATTY	3 cut(s)	426, 617, 1108
AspLEI	GCGC	3 cut(s)	37, 227, 1220
AspS9I	GGNCC	4 cut(s)	218, 610, 899, 1050
AsuC2I	CCSGG	2 cut(s)	222, 897
AsuHPI	GGTGA	5 cut(s)	88, 434, 885, 1170, 1224
AvaI	CYCGRG	2 cut(s)	606, 1189
AvaII	GGWCC	3 cut(s)	218, 610, 1050
BaeGI	GKGCMC	1 cut(s)	8
BanII	GRGCYC	1 cut(s)	301
BbsI	GAAGAC	1 cut(s)	348
Bbv12I	GWGCWC	1 cut(s)	301
BbvI	GCAGC	2 cut(s)	307, 1304
BccI	CCATC	2 cut(s)	910, 1177
BceAI	ACGGC	2 cut(s)	364, 659
BcgI	CGANNNNNNTGC	2 cut(s)	868, 902
BcnI	CCSGG	2 cut(s)	222, 897
BcoDI	GTCTC	3 cut(s)	996, 1047, 1131
BfaI	CTAG	2 cut(s)	933, 1023
BfmI	CTRYAG	3 cut(s)	540, 727, 1279
BfoI	RGCGCY	1 cut(s)	38
BfuAI	ACCTGC	1 cut(s)	1110
BisI	GCNGC	2 cut(s)	321, 1293
BlpI	GCTNAGC	1 cut(s)	177
BlsI	GCNGC	2 cut(s)	322, 1294
Bme1390I	CCNGG	2 cut(s)	222, 897
Bme18I	GGWCC	3 cut(s)	218, 610, 1050
BmeT110I	CYCGRG	2 cut(s)	606, 1189
BmgT120I	GGNCC	4 cut(s)	218, 610, 899, 1050
BmiI	GGNNCC	1 cut(s)	611
BmrFI	CCNGG	2 cut(s)	222, 897
BmrI	ACTGGG	1 cut(s)	818
BmsI	GCATC	6 cut(s)	55, 178, 295, 829, 959, 1276
BmuI	ACTGGG	1 cut(s)	818
BpiI	GAAGAC	1 cut(s)	348
Bpu1102I	GCTNAGC	1 cut(s)	177
BpuEI	CTTGAG	4 cut(s)	285, 582, 740, 1264
BpuMI	CCSGG	2 cut(s)	222, 897
BsaHI	GRCGYC	1 cut(s)	351
BsaI	GGTCTC	2 cut(s)	996, 1131
BsaXI	ACNNNNNCTCC	2 cut(s)	1288, 1318
Bse1I	ACTGG	4 cut(s)	824, 951, 1063, 1254
Bse3DI	GCAATG	3 cut(s)	657, 884, 1231
BseGI	GGATG	7 cut(s)	70, 633, 902, 1006, 1032, 1115, 1267
BseMI	GCAATG	3 cut(s)	657, 884, 1231
BseMII	CTCAG	3 cut(s)	168, 504, 1107
BseNI	ACTGG	4 cut(s)	824, 951, 1063, 1254
BseRI	GAGGAG	4 cut(s)	276, 296, 907, 1147
BseSI	GKGCMC	1 cut(s)	8
BseXI	GCAGC	2 cut(s)	307, 1304
BseYI	CCCAGC	1 cut(s)	308
BsgI	GTGCAG	1 cut(s)	676
Bsh1236I	CGCG	1 cut(s)	227
BshFI	GGCC	3 cut(s)	595, 900, 914
BsiHKAI	GWGCWC	1 cut(s)	301
BsiHKCI	CYCGRG	2 cut(s)	606, 1189
BsiSI	CCGG	4 cut(s)	134, 216, 221, 897
BslFI	GGGAC	1 cut(s)	1051
BsmAI	GTCTC	3 cut(s)	996, 1047, 1131
BsmFI	GGGAC	1 cut(s)	1051
BsmI	GAATGC	1 cut(s)	1340
BsnI	GGCC	3 cut(s)	595, 900, 914
Bso31I	GGTCTC	2 cut(s)	996, 1131
BsoBI	CYCGRG	2 cut(s)	606, 1189
Bsp1286I	GDGCHC	2 cut(s)	8, 301
Bsp143I	GATC	2 cut(s)	859, 938
Bsp1720I	GCTNAGC	1 cut(s)	177
BspACI	CCGC	3 cut(s)	62, 1060, 1105
BspANI	GGCC	3 cut(s)	595, 900, 914
BspCNI	CTCAG	3 cut(s)	169, 503, 1106
BspFNI	CGCG	1 cut(s)	227
BspLI	GGNNCC	1 cut(s)	611
BspMAI	CTGCAG	1 cut(s)	544
BspMI	ACCTGC	1 cut(s)	1110
BspQI	GCTCTTC	1 cut(s)	289
BspTNI	GGTCTC	2 cut(s)	996, 1131
BsrDI	GCAATG	3 cut(s)	657, 884, 1231
BsrI	ACTGG	4 cut(s)	824, 951, 1063, 1254
BssMI	GATC	2 cut(s)	859, 938
BssNI	GRCGYC	1 cut(s)	351
Bst4CI	ACNGT	4 cut(s)	731, 927, 1204, 1280
Bst6I	CTCTTC	3 cut(s)	253, 289, 1332
BstACI	GRCGYC	1 cut(s)	351
BstAPI	GCANNNNNTGC	1 cut(s)	1226
BstC8I	GCNNGC	5 cut(s)	193, 478, 531, 833, 1222
BstDEI	CTNAG	5 cut(s)	177, 490, 746, 1046, 1093
BstF5I	GGATG	7 cut(s)	70, 633, 902, 1006, 1032, 1115, 1267
BstFNI	CGCG	1 cut(s)	227
BstH2I	RGCGCY	1 cut(s)	38
BstHHI	GCGC	3 cut(s)	37, 227, 1220
BstKTI	GATC	2 cut(s)	862, 941
BstMAI	GTCTC	3 cut(s)	996, 1047, 1131
BstMBI	GATC	2 cut(s)	859, 938
BstMWI	GCNNNNNNNGC	7 cut(s)	128, 305, 539, 548, 576, 828, 1226
BstNSI	RCATGY	3 cut(s)	195, 398, 708
BstSCI	CCNGG	2 cut(s)	220, 895
BstSFI	CTRYAG	3 cut(s)	540, 727, 1279
BstSLI	GKGCMC	1 cut(s)	8
BstUI	CGCG	1 cut(s)	227
BstV1I	GCAGC	2 cut(s)	307, 1304
BstV2I	GAAGAC	1 cut(s)	348
BsuRI	GGCC	3 cut(s)	595, 900, 914
BtgZI	GCGATG	1 cut(s)	560
BtsCI	GGATG	7 cut(s)	70, 633, 902, 1006, 1032, 1115, 1267
BtsI	GCAGTG	1 cut(s)	161
BtsIMutI	CAGTG	5 cut(s)	161, 736, 944, 1181, 1276
BveI	ACCTGC	1 cut(s)	1110
Cac8I	GCNNGC	5 cut(s)	193, 478, 531, 833, 1222
CfoI	GCGC	3 cut(s)	37, 227, 1220
Cfr13I	GGNCC	4 cut(s)	218, 610, 899, 1050
CpoI	CGGWCCG	1 cut(s)	218
CseI	GACGC	1 cut(s)	340
Csp6I	GTAC	4 cut(s)	439, 923, 974, 1205
CspI	CGGWCCG	1 cut(s)	218
CviQI	GTAC	4 cut(s)	439, 923, 974, 1205
DdeI	CTNAG	5 cut(s)	177, 490, 746, 1046, 1093
DpnI	GATC	2 cut(s)	861, 940
DpnII	GATC	2 cut(s)	859, 938
DrdI	GACNNNNNNGTC	1 cut(s)	350
DseDI	GACNNNNNNGTC	1 cut(s)	350
Eam1104I	CTCTTC	3 cut(s)	253, 289, 1332
EarI	CTCTTC	3 cut(s)	253, 289, 1332
EciI	GGCGGA	2 cut(s)	77, 1049
Ecl136II	GAGCTC	1 cut(s)	299
Eco147I	AGGCCT	1 cut(s)	595
Eco24I	GRGCYC	1 cut(s)	301
Eco31I	GGTCTC	2 cut(s)	996, 1131
Eco32I	GATATC	1 cut(s)	1196
Eco47I	GGWCC	3 cut(s)	218, 610, 1050
Eco53kI	GAGCTC	1 cut(s)	299
Eco57I	CTGAAG	3 cut(s)	681, 855, 942
Eco88I	CYCGRG	2 cut(s)	606, 1189
EcoICRI	GAGCTC	1 cut(s)	299
EcoO109I	RGGNCCY	1 cut(s)	1050
EcoRI	GAATTC	1 cut(s)	1108
EcoRV	GATATC	1 cut(s)	1196
EcoT22I	ATGCAT	1 cut(s)	193
EcoT38I	GRGCYC	1 cut(s)	301
FalI	AAGNNNNNCTT	4 cut(s)	979, 1011, 1213, 1245
FaqI	GGGAC	1 cut(s)	1051
FblI	GTMKAC	1 cut(s)	1098
Fnu4HI	GCNGC	2 cut(s)	321, 1293
FokI	GGATG	7 cut(s)	77, 640, 889, 993, 1019, 1102, 1254
FriOI	GRGCYC	1 cut(s)	301
Fsp4HI	GCNGC	2 cut(s)	321, 1293
FspBI	CTAG	2 cut(s)	933, 1023
GlaI	GCGC	3 cut(s)	36, 226, 1219
GluI	GCNGC	2 cut(s)	321, 1293
GsaI	CCCAGC	1 cut(s)	312
HaeII	RGCGCY	1 cut(s)	38
HaeIII	GGCC	3 cut(s)	595, 900, 914
HapII	CCGG	4 cut(s)	134, 216, 221, 897
HgaI	GACGC	1 cut(s)	340
HhaI	GCGC	3 cut(s)	37, 227, 1220
Hin1I	GRCGYC	1 cut(s)	351
Hin6I	GCGC	3 cut(s)	35, 225, 1218
HinP1I	GCGC	3 cut(s)	35, 225, 1218
HindIII	AAGCTT	2 cut(s)	414, 600
HinfI	GANTC	7 cut(s)	13, 447, 751, 791, 875, 980, 1286
HpaII	CCGG	4 cut(s)	134, 216, 221, 897
HphI	GGTGA	5 cut(s)	88, 434, 885, 1170, 1224
Hpy166II	GTNNAC	2 cut(s)	790, 1099
Hpy188I	TCNGA	4 cut(s)	614, 874, 969, 1200
Hpy188III	TCNNGA	5 cut(s)	79, 302, 1191, 1243, 1331
Hpy8I	GTNNAC	2 cut(s)	790, 1099
Hpy99I	CGWCG	1 cut(s)	350
HpyAV	CCTTC	3 cut(s)	526, 1066, 1080
HpyCH4III	ACNGT	4 cut(s)	731, 927, 1204, 1280
HpyCH4IV	ACGT	2 cut(s)	93, 114
HpyF10VI	GCNNNNNNNGC	7 cut(s)	128, 305, 539, 548, 576, 828, 1226
HpyF3I	CTNAG	5 cut(s)	177, 490, 746, 1046, 1093
HpySE526I	ACGT	2 cut(s)	93, 114
Hsp92I	GRCGYC	1 cut(s)	351
HspAI	GCGC	3 cut(s)	35, 225, 1218
Kzo9I	GATC	2 cut(s)	859, 938
LguI	GCTCTTC	1 cut(s)	289
LmnI	GCTCC	3 cut(s)	87, 136, 242
Lsp1109I	GCAGC	2 cut(s)	307, 1304
LweI	GCATC	6 cut(s)	55, 178, 295, 829, 959, 1276
MaeI	CTAG	2 cut(s)	933, 1023
MaeII	ACGT	2 cut(s)	93, 114
MaeIII	GTNAC	3 cut(s)	94, 700, 764
MalI	GATC	2 cut(s)	861, 940
MboI	GATC	2 cut(s)	859, 938
MboII	GAAGA	6 cut(s)	270, 306, 353, 948, 1242, 1319
MfeI	CAATTG	1 cut(s)	324
MhlI	GDGCHC	2 cut(s)	8, 301
MluCI	AATT	7 cut(s)	124, 229, 324, 426, 471, 617, 1108
MlyI	GAGTC	3 cut(s)	441, 785, 1295
MmeI	TCCRAC	1 cut(s)	715
Mph1103I	ATGCAT	1 cut(s)	193
MslI	CAYNNNNRTG	3 cut(s)	455, 461, 1281
MspA1I	CMGCKG	1 cut(s)	380
MspI	CCGG	4 cut(s)	134, 216, 221, 897
MspR9I	CCNGG	2 cut(s)	222, 897
MunI	CAATTG	1 cut(s)	324
Mva1269I	GAATGC	1 cut(s)	1340
MvnI	CGCG	1 cut(s)	227
MwoI	GCNNNNNNNGC	7 cut(s)	128, 305, 539, 548, 576, 828, 1226
NciI	CCSGG	2 cut(s)	222, 897
NdeII	GATC	2 cut(s)	859, 938
NlaIV	GGNNCC	1 cut(s)	611
NmuCI	GTSAC	2 cut(s)	94, 764
NsiI	ATGCAT	1 cut(s)	193
NspI	RCATGY	3 cut(s)	195, 398, 708
OliI	CACNNNNGTG	1 cut(s)	1281
PaeI	GCATGC	1 cut(s)	195
PaeR7I	CTCGAG	1 cut(s)	1189
PceI	AGGCCT	1 cut(s)	595
PciI	ACATGT	1 cut(s)	704
PciSI	GCTCTTC	1 cut(s)	289
PcsI	WCGNNNNNNNCGW	1 cut(s)	583
PctI	GAATGC	1 cut(s)	1340
PfeI	GAWTC	4 cut(s)	13, 751, 875, 980
PflFI	GACNNNGTC	1 cut(s)	350
PkrI	GCNGC	2 cut(s)	322, 1294
PleI	GAGTC	3 cut(s)	441, 785, 1294
PpsI	GAGTC	3 cut(s)	441, 785, 1294
PpuMI	RGGWCCY	1 cut(s)	1050
PscI	ACATGT	1 cut(s)	704
Psp124BI	GAGCTC	1 cut(s)	301
Psp5II	RGGWCCY	1 cut(s)	1050
PspFI	CCCAGC	1 cut(s)	308
PspN4I	GGNNCC	1 cut(s)	611
PspPI	GGNCC	4 cut(s)	218, 610, 899, 1050
PspPPI	RGGWCCY	1 cut(s)	1050
PstI	CTGCAG	1 cut(s)	544
PsyI	GACNNNGTC	1 cut(s)	350
PvuII	CAGCTG	1 cut(s)	380
RsaI	GTAC	4 cut(s)	440, 924, 975, 1206
RsaNI	GTAC	4 cut(s)	439, 923, 974, 1205
RseI	CAYNNNNRTG	3 cut(s)	455, 461, 1281
Rsr2I	CGGWCCG	1 cut(s)	218
RsrII	CGGWCCG	1 cut(s)	218
SacI	GAGCTC	1 cut(s)	301
SapI	GCTCTTC	1 cut(s)	289
SatI	GCNGC	2 cut(s)	321, 1293
Sau3AI	GATC	2 cut(s)	859, 938
Sau96I	GGNCC	4 cut(s)	218, 610, 899, 1050
SchI	GAGTC	3 cut(s)	441, 785, 1295
ScrFI	CCNGG	2 cut(s)	222, 897
SduI	GDGCHC	2 cut(s)	8, 301
SfaNI	GCATC	6 cut(s)	55, 178, 295, 829, 959, 1276
SfcI	CTRYAG	3 cut(s)	540, 727, 1279
Sfr274I	CTCGAG	1 cut(s)	1189
SinI	GGWCC	3 cut(s)	218, 610, 1050
SlaI	CTCGAG	1 cut(s)	1189
SmiMI	CAYNNNNRTG	3 cut(s)	455, 461, 1281
SmlI	CTYRAG	5 cut(s)	300, 597, 719, 1189, 1243
SmoI	CTYRAG	5 cut(s)	300, 597, 719, 1189, 1243
SphI	GCATGC	1 cut(s)	195
Sse9I	AATT	7 cut(s)	124, 229, 324, 426, 471, 617, 1108
SseBI	AGGCCT	1 cut(s)	595
SsiI	CCGC	3 cut(s)	62, 1060, 1105
SspMI	CTAG	2 cut(s)	933, 1023
SstI	GAGCTC	1 cut(s)	301
StuI	AGGCCT	1 cut(s)	595
StyD4I	CCNGG	2 cut(s)	220, 895
TaaI	ACNGT	4 cut(s)	731, 927, 1204, 1280
TaiI	ACGT	2 cut(s)	96, 117
TaqI	TCGA	4 cut(s)	108, 197, 878, 1190
TasI	AATT	7 cut(s)	124, 229, 324, 426, 471, 617, 1108
TatI	WGTACW	1 cut(s)	922
TfiI	GAWTC	4 cut(s)	13, 751, 875, 980
TscAI	CASTG	5 cut(s)	161, 736, 951, 1188, 1283
TseFI	GTSAC	2 cut(s)	94, 764
TseI	GCWGC	2 cut(s)	320, 1292
Tsp45I	GTSAC	2 cut(s)	94, 764
TspDTI	ATGAA	6 cut(s)	26, 354, 508, 611, 764, 795
TspRI	CASTG	5 cut(s)	161, 736, 951, 1188, 1283
Tth111I	GACNNNGTC	1 cut(s)	350
VpaK11BI	GGWCC	3 cut(s)	218, 610, 1050
XapI	RAATTY	3 cut(s)	426, 617, 1108
XceI	RCATGY	3 cut(s)	195, 398, 708
XhoI	CTCGAG	1 cut(s)	1189
XmiI	GTMKAC	1 cut(s)	1098
XspI	CTAG	2 cut(s)	933, 1023
Zsp2I	ATGCAT	1 cut(s)	193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.