Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008068

Physical Location & Seq

Genomic Coordinates Forward (+)

28214368 .. 28221285

Gene Structure 6918 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1219500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1353 bp

ATGAGCACTATGAATCTGGCGGTGGTTCTGGTGGCGGTGGCGGTGGTTCTGTTCGGCCGATTTTGCATCACCAAGGCCAATGTGGTGCTGATGGGGAACAACGTCACTCTCTCTTTCGACGACAAGGAAGCTACTTTTGCTCCGGAGGTTAAGGGTTCCGGGGAATGCGGAGTATTGTATTTGGCCGAGCCTCCTCATGCGTGCTCGACATTGACTAATCAAGTCGAACAAGGCACGAATTATAGCTCCCCCTTCGTTTTGATCGTAAGAGGAGGGTGTAGTTTTGAGGATAAAGTTAGAAGAGCACAAAAGGCCGGCTTCAAAGCTGCAATTATCTACGACAATGAAGATAGCAACATCTTGGTTTCAATGGCAGGAAGCTCAGCTGGTATAAAAATATACGCCGTGTTCGTTTCGAAAACTTCTGGTGAACTACTAAAGAAGTACATTGGACTTACTAACATGGAGCTGTGGCTAATTGCAAGCTTTGAGAATACAGCATGGTCAATCATGGCAATCTCTTTCATTTCATTGCTTGCCATGTCTGCAGTGCTGGCTACTTGTTTTTTCGTTCGCAGGCATCGAATAAGACGAGAGAGGCCTCAAGCTTTTCGTGTCCAAGAATTTCATGGGATGAGCAAACGCTTAGTAAAAGCAATGCCAAGTTTGAAATTTACCACTGTTTTAGAGGACAACTGCACATCAAGCACATGTGCTATATGTCTTGAGGACTATAACTTTGGAGAAAAACTCAGGATTCTGCCATGTCGCCATAAGTTTCATGCTTTCTGTGTGGATTCTTGGCTTACTTCATGGAGAACCTTTTGTCCAGTTTGCAAGCGTGATGCAAGAGCTAATACGGGTGATCTACCCGCTTCAGAATCCACACCATTGCTTTCGTCCGGCCCATCCTCCGTGGCATCTTCCGTACTTTCATCTATGAGATCATCAATAGCATCATCTTCAGCAATACAGATAGGACCATCATTGTCACGGAACCCTTCATTGTCTCATTCTCACTCTTATGCTAGCACAGGTCACATTCAGCAGTCTCTTAGGTCCTCCTATCGCCAGTCCCCTTCTATTAGTGCAAGCAGAAGTTCAATGGACCTCCGAAATGCCTCGTCTCAAAGGTCCCATGTTTCCTATTTTGTTTCACCCCATTCCATGGGTTACCCAACTTTATTACCCCCGAATTCAAGATACATGTCTGTTGGACACATTCCAAGCCCTAGCAATGCATCACCAAGCTACATGAGCTCTTCCAATCATCGACAGCATCCACTACATTGTAGTGAATCAGCTGCAACGTTTTCTCCTTTTTCTTCTGCTCAATCTCTTCCTGAATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000209 GO:0000322 GO:0000325 GO:0000326 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0031090 GO:0031984 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0070647 GO:0071704 GO:0098588 GO:0098791 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA zf-RING_2

Protein Analysis

451

Amino Acids

49.06

Weight (kDa)

9.17

Isoelectric Point (pI)

68.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	62 - 146	2.7e-13	PA domain
zf-RING_2	PF13639	237 - 280	4.6e-12	Ring finger domain
zf-RING_11	PF17123	238 - 265	7e-09	RING-like zinc finger
zf-C3HC4	PF00097	238 - 279	1.3e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71980
fragaria_vesca	FvH4_5g30950 FvH4_5g30950 FvH4_5g30950 FvH4_5g30950 FvH4_5g30950
malus_domestica	MD08G1219500.v1.1 MD15G1408100.v1.1
prunus_persica	Prupe.1G553100_v2.0.a1
pyrus_communis	pycom08g19050 pycom15g36420 pycom15g36430
rosa_chinensis	RchiOBHm_Chr7g0226311
rosa_laevigata	RLG00000001752
rosa_multiflora	Rmu_sc0004322.1_g000023
rosa_roxburghii	Rroxscaffold_3G00233040
rosa_rugosa	Rorug07G0236300 Rorug07G0236400 Rorug07G0236500
rosa_samantha	Rh7AG383500 Rh7BG370300 Rh7CG403100 Rh7DG381900
rosa_wichuraiana	Rw7G032120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1168
AccIII	TCCGGA	1 cut(s)	142
AciI	CCGC	5 cut(s)	20, 35, 41, 168, 873
AclI	AACGTT	1 cut(s)	1310
AcoI	YGGCCR	2 cut(s)	55, 183
AcsI	RAATTY	3 cut(s)	623, 671, 1195
AcuI	CTGAAG	2 cut(s)	861, 948
AfaI	GTAC	2 cut(s)	446, 930
AfiI	CCNNNNNNNGG	1 cut(s)	1168
AflIII	ACRYGT	2 cut(s)	710, 1206
AgsI	TTSAA	5 cut(s)	322, 369, 670, 1104, 1200
AloI	GAACNNNNNNTCC	2 cut(s)	811, 843
Alw21I	GWGCWC	4 cut(s)	8, 206, 307, 1262
Alw26I	GTCTC	3 cut(s)	1014, 1056, 1131
Aor13HI	TCCGGA	1 cut(s)	142
AoxI	GGCC	6 cut(s)	55, 75, 183, 312, 599, 904
ApeKI	GCWGC	2 cut(s)	326, 1304
ApoI	RAATTY	3 cut(s)	623, 671, 1195
ArsI	GACNNNNNNTTYG	2 cut(s)	722, 754
AspS9I	GGNCC	5 cut(s)	905, 980, 1059, 1108, 1134
AsuC2I	CCSGG	1 cut(s)	160
AsuHPI	GGTGA	5 cut(s)	61, 440, 875, 1149, 1236
AsuII	TTCGAA	1 cut(s)	416
AsuNHI	GCTAGC	1 cut(s)	1028
AvaII	GGWCC	4 cut(s)	980, 1059, 1108, 1134
BanII	GRGCYC	1 cut(s)	1262
Bbv12I	GWGCWC	4 cut(s)	8, 206, 307, 1262
BbvI	GCAGC	2 cut(s)	313, 1291
BccI	CCATC	3 cut(s)	85, 916, 991
BceAI	ACGGC	1 cut(s)	389
BcnI	CCSGG	1 cut(s)	160
BcoDI	GTCTC	3 cut(s)	1014, 1056, 1131
BfaI	CTAG	2 cut(s)	1029, 1233
BfmI	CTRYAG	1 cut(s)	546
BisI	GCNGC	2 cut(s)	327, 1305
BlpI	GCTNAGC	1 cut(s)	382
BlsI	GCNGC	2 cut(s)	328, 1306
Bme1390I	CCNGG	1 cut(s)	160
Bme18I	GGWCC	4 cut(s)	980, 1059, 1108, 1134
BmgT120I	GGNCC	5 cut(s)	905, 980, 1059, 1108, 1134
BmiI	GGNNCC	3 cut(s)	157, 998, 1136
BmrFI	CCNGG	1 cut(s)	160
BmsI	GCATC	7 cut(s)	75, 589, 835, 929, 965, 1250, 1288
BmtI	GCTAGC	1 cut(s)	1032
BplI	GAGNNNNNCTC	2 cut(s)	735, 767
Bpu1102I	GCTNAGC	1 cut(s)	382
Bpu14I	TTCGAA	1 cut(s)	416
BpuEI	CTTGAG	2 cut(s)	588, 746
BpuMI	CCSGG	1 cut(s)	160
BsaJI	CCNNGG	4 cut(s)	72, 159, 915, 1167
BsaWI	WCCGGW	1 cut(s)	142
BsaXI	ACNNNNNCTCC	4 cut(s)	124, 154, 1300, 1330
Bsc4I	CCNNNNNNNGG	1 cut(s)	1168
Bse118I	RCCGGY	1 cut(s)	314
Bse1I	ACTGG	2 cut(s)	830, 1072
Bse3DI	GCAATG	4 cut(s)	530, 663, 890, 1243
BseAI	TCCGGA	1 cut(s)	142
BseDI	CCNNGG	4 cut(s)	72, 159, 915, 1167
BseGI	GGATG	3 cut(s)	639, 908, 1279
BseLI	CCNNNNNNNGG	1 cut(s)	1168
BseMI	GCAATG	4 cut(s)	530, 663, 890, 1243
BseMII	CTCAG	2 cut(s)	396, 766
BseNI	ACTGG	2 cut(s)	830, 1072
BseRI	GAGGAG	2 cut(s)	183, 285
BseX3I	CGGCCG	1 cut(s)	55
BseXI	GCAGC	2 cut(s)	313, 1291
BsgI	GTGCAG	1 cut(s)	682
Bsh1285I	CGRYCG	1 cut(s)	58
BshFI	GGCC	6 cut(s)	57, 77, 185, 314, 601, 906
BsiEI	CGRYCG	1 cut(s)	58
BsiHKAI	GWGCWC	4 cut(s)	8, 206, 307, 1262
BsiSI	CCGG	4 cut(s)	143, 159, 315, 903
BslFI	GGGAC	2 cut(s)	1060, 1120
BslI	CCNNNNNNNGG	1 cut(s)	1168
BsmAI	GTCTC	3 cut(s)	1014, 1056, 1131
BsmBI	CGTCTC	1 cut(s)	1131
BsmFI	GGGAC	2 cut(s)	1060, 1120
BsmI	GAATGC	2 cut(s)	170, 1352
BsnI	GGCC	6 cut(s)	57, 77, 185, 314, 601, 906
Bsp119I	TTCGAA	1 cut(s)	416
Bsp1286I	GDGCHC	4 cut(s)	8, 206, 307, 1262
Bsp13I	TCCGGA	1 cut(s)	142
Bsp143I	GATC	3 cut(s)	261, 865, 944
Bsp1720I	GCTNAGC	1 cut(s)	382
Bsp19I	CCATGG	1 cut(s)	1167
BspACI	CCGC	5 cut(s)	20, 35, 41, 168, 873
BspANI	GGCC	6 cut(s)	57, 77, 185, 314, 601, 906
BspCNI	CTCAG	2 cut(s)	395, 765
BspEI	TCCGGA	1 cut(s)	142
BspLI	GGNNCC	3 cut(s)	157, 998, 1136
BspMAI	CTGCAG	1 cut(s)	550
BspOI	GCTAGC	1 cut(s)	1032
BspQI	GCTCTTC	2 cut(s)	295, 1267
BspT104I	TTCGAA	1 cut(s)	416
BsrDI	GCAATG	4 cut(s)	530, 663, 890, 1243
BsrFI	RCCGGY	1 cut(s)	314
BsrI	ACTGG	2 cut(s)	830, 1072
BssAI	RCCGGY	1 cut(s)	314
BssECI	CCNNGG	4 cut(s)	72, 159, 915, 1167
BssMI	GATC	3 cut(s)	261, 865, 944
BssT1I	CCWWGG	2 cut(s)	72, 1167
Bst4CI	ACNGT	1 cut(s)	682
Bst6I	CTCTTC	3 cut(s)	295, 1267, 1344
BstBI	TTCGAA	1 cut(s)	416
BstC8I	GCNNGC	9 cut(s)	202, 316, 484, 537, 555, 578, 839, 1030, 1093
BstDEI	CTNAG	4 cut(s)	382, 646, 752, 1055
BstDSI	CCRYGG	2 cut(s)	915, 1167
BstEII	GGTNACC	1 cut(s)	1172
BstF5I	GGATG	3 cut(s)	639, 908, 1279
BstKTI	GATC	3 cut(s)	264, 868, 947
BstMAI	GTCTC	3 cut(s)	1014, 1056, 1131
BstMBI	GATC	3 cut(s)	261, 865, 944
BstMCI	CGRYCG	1 cut(s)	58
BstMWI	GCNNNNNNNGC	7 cut(s)	63, 137, 311, 545, 554, 705, 1257
BstNSI	RCATGY	2 cut(s)	714, 1210
BstPI	GGTNACC	1 cut(s)	1172
BstSCI	CCNGG	1 cut(s)	158
BstSFI	CTRYAG	1 cut(s)	546
BstV1I	GCAGC	2 cut(s)	313, 1291
BstZI	CGGCCG	1 cut(s)	55
BsuRI	GGCC	6 cut(s)	57, 77, 185, 314, 601, 906
BtgI	CCRYGG	2 cut(s)	915, 1167
BtsCI	GGATG	3 cut(s)	639, 908, 1279
BtsI	GCAGTG	1 cut(s)	555
BtsIMutI	CAGTG	2 cut(s)	555, 678
Cac8I	GCNNGC	9 cut(s)	202, 316, 484, 537, 555, 578, 839, 1030, 1093
Cfr10I	RCCGGY	1 cut(s)	314
Cfr13I	GGNCC	5 cut(s)	905, 980, 1059, 1108, 1134
Csp6I	GTAC	2 cut(s)	445, 929
CviQI	GTAC	2 cut(s)	445, 929
DdeI	CTNAG	4 cut(s)	382, 646, 752, 1055
DpnI	GATC	3 cut(s)	263, 867, 946
DpnII	GATC	3 cut(s)	261, 865, 944
EaeI	YGGCCR	2 cut(s)	55, 183
EagI	CGGCCG	1 cut(s)	55
Eam1104I	CTCTTC	3 cut(s)	295, 1267, 1344
EarI	CTCTTC	3 cut(s)	295, 1267, 1344
Ecl136II	GAGCTC	1 cut(s)	1260
EclXI	CGGCCG	1 cut(s)	55
Eco130I	CCWWGG	2 cut(s)	72, 1167
Eco147I	AGGCCT	1 cut(s)	601
Eco24I	GRGCYC	1 cut(s)	1262
Eco47I	GGWCC	4 cut(s)	980, 1059, 1108, 1134
Eco52I	CGGCCG	1 cut(s)	55
Eco53kI	GAGCTC	1 cut(s)	1260
Eco57I	CTGAAG	2 cut(s)	861, 948
Eco91I	GGTNACC	1 cut(s)	1172
EcoICRI	GAGCTC	1 cut(s)	1260
EcoO109I	RGGNCCY	2 cut(s)	1059, 1134
EcoO65I	GGTNACC	1 cut(s)	1172
EcoRI	GAATTC	1 cut(s)	1195
EcoT14I	CCWWGG	2 cut(s)	72, 1167
EcoT22I	ATGCAT	1 cut(s)	1243
EcoT38I	GRGCYC	1 cut(s)	1262
ErhI	CCWWGG	2 cut(s)	72, 1167
Esp3I	CGTCTC	1 cut(s)	1131
FalI	AAGNNNNNCTT	2 cut(s)	302, 334
FaqI	GGGAC	2 cut(s)	1060, 1120
FauI	CCCGC	1 cut(s)	880
Fnu4HI	GCNGC	2 cut(s)	327, 1305
FokI	GGATG	3 cut(s)	646, 895, 1266
FriOI	GRGCYC	1 cut(s)	1262
Fsp4HI	GCNGC	2 cut(s)	327, 1305
FspBI	CTAG	2 cut(s)	1029, 1233
GluI	GCNGC	2 cut(s)	327, 1305
HaeIII	GGCC	6 cut(s)	57, 77, 185, 314, 601, 906
HapII	CCGG	4 cut(s)	143, 159, 315, 903
HindIII	AAGCTT	2 cut(s)	484, 606
HinfI	GANTC	5 cut(s)	13, 757, 797, 881, 1298
HpaII	CCGG	4 cut(s)	143, 159, 315, 903
HphI	GGTGA	5 cut(s)	61, 440, 875, 1149, 1236
Hpy166II	GTNNAC	1 cut(s)	431
Hpy188I	TCNGA	2 cut(s)	880, 1115
Hpy188III	TCNNGA	5 cut(s)	143, 725, 754, 1200, 1343
Hpy8I	GTNNAC	1 cut(s)	431
Hpy99I	CGWCG	1 cut(s)	122
HpyAV	CCTTC	3 cut(s)	262, 1011, 1089
HpyCH4III	ACNGT	1 cut(s)	682
HpyCH4IV	ACGT	2 cut(s)	102, 1310
HpyF10VI	GCNNNNNNNGC	7 cut(s)	63, 137, 311, 545, 554, 705, 1257
HpyF3I	CTNAG	4 cut(s)	382, 646, 752, 1055
HpySE526I	ACGT	2 cut(s)	102, 1310
Kpn2I	TCCGGA	1 cut(s)	142
KroI	GCCGGC	1 cut(s)	314
KroNI	GCCGGC	1 cut(s)	316
Kzo9I	GATC	3 cut(s)	261, 865, 944
LguI	GCTCTTC	2 cut(s)	295, 1267
LmnI	GCTCC	3 cut(s)	145, 251, 466
Lsp1109I	GCAGC	2 cut(s)	313, 1291
LweI	GCATC	7 cut(s)	75, 589, 835, 929, 965, 1250, 1288
MaeI	CTAG	2 cut(s)	1029, 1233
MaeII	ACGT	2 cut(s)	102, 1310
MaeIII	GTNAC	4 cut(s)	103, 990, 1037, 1172
MalI	GATC	3 cut(s)	263, 867, 946
MboI	GATC	3 cut(s)	261, 865, 944
MboII	GAAGA	7 cut(s)	312, 359, 915, 954, 1254, 1317, 1331
MhlI	GDGCHC	4 cut(s)	8, 206, 307, 1262
MluCI	AATT	6 cut(s)	238, 330, 477, 623, 671, 1195
MmeI	TCCRAC	1 cut(s)	1195
Mph1103I	ATGCAT	1 cut(s)	1243
MroI	TCCGGA	1 cut(s)	142
MroNI	GCCGGC	1 cut(s)	314
MseI	TTAA	1 cut(s)	150
MslI	CAYNNNNRTG	2 cut(s)	1023, 1293
MspA1I	CMGCKG	2 cut(s)	386, 1304
MspI	CCGG	4 cut(s)	143, 159, 315, 903
MspR9I	CCNGG	1 cut(s)	160
Mva1269I	GAATGC	2 cut(s)	170, 1352
MwoI	GCNNNNNNNGC	7 cut(s)	63, 137, 311, 545, 554, 705, 1257
NaeI	GCCGGC	1 cut(s)	316
NciI	CCSGG	1 cut(s)	160
NcoI	CCATGG	1 cut(s)	1167
NdeII	GATC	3 cut(s)	261, 865, 944
NgoMIV	GCCGGC	1 cut(s)	314
NheI	GCTAGC	1 cut(s)	1028
NlaIV	GGNNCC	3 cut(s)	157, 998, 1136
NmeAIII	GCCGAG	1 cut(s)	211
NmuCI	GTSAC	3 cut(s)	103, 990, 1037
NsiI	ATGCAT	1 cut(s)	1243
NspI	RCATGY	2 cut(s)	714, 1210
NspV	TTCGAA	1 cut(s)	416
PceI	AGGCCT	1 cut(s)	601
PciI	ACATGT	2 cut(s)	710, 1206
PciSI	GCTCTTC	2 cut(s)	295, 1267
PcsI	WCGNNNNNNNCGW	4 cut(s)	261, 408, 580, 589
PctI	GAATGC	2 cut(s)	170, 1352
PdiI	GCCGGC	1 cut(s)	316
PfeI	GAWTC	5 cut(s)	13, 757, 797, 881, 1298
PflMI	CCANNNNNTGG	1 cut(s)	1168
PkrI	GCNGC	2 cut(s)	328, 1306
PpuMI	RGGWCCY	2 cut(s)	1059, 1134
PscI	ACATGT	2 cut(s)	710, 1206
Psp124BI	GAGCTC	1 cut(s)	1262
Psp1406I	AACGTT	1 cut(s)	1310
Psp5II	RGGWCCY	2 cut(s)	1059, 1134
PspEI	GGTNACC	1 cut(s)	1172
PspN4I	GGNNCC	3 cut(s)	157, 998, 1136
PspPI	GGNCC	5 cut(s)	905, 980, 1059, 1108, 1134
PspPPI	RGGWCCY	2 cut(s)	1059, 1134
PstI	CTGCAG	1 cut(s)	550
PvuII	CAGCTG	2 cut(s)	386, 1304
RsaI	GTAC	2 cut(s)	446, 930
RsaNI	GTAC	2 cut(s)	445, 929
RseI	CAYNNNNRTG	2 cut(s)	1023, 1293
SacI	GAGCTC	1 cut(s)	1262
SapI	GCTCTTC	2 cut(s)	295, 1267
SaqAI	TTAA	1 cut(s)	150
SatI	GCNGC	2 cut(s)	327, 1305
Sau3AI	GATC	3 cut(s)	261, 865, 944
Sau96I	GGNCC	5 cut(s)	905, 980, 1059, 1108, 1134
ScrFI	CCNGG	1 cut(s)	160
SduI	GDGCHC	4 cut(s)	8, 206, 307, 1262
SfaNI	GCATC	7 cut(s)	75, 589, 835, 929, 965, 1250, 1288
SfcI	CTRYAG	1 cut(s)	546
SfuI	TTCGAA	1 cut(s)	416
SinI	GGWCC	4 cut(s)	980, 1059, 1108, 1134
SmiMI	CAYNNNNRTG	2 cut(s)	1023, 1293
SmlI	CTYRAG	2 cut(s)	603, 725
SmoI	CTYRAG	2 cut(s)	603, 725
Sse9I	AATT	6 cut(s)	238, 330, 477, 623, 671, 1195
SseBI	AGGCCT	1 cut(s)	601
SsiI	CCGC	5 cut(s)	20, 35, 41, 168, 873
SspMI	CTAG	2 cut(s)	1029, 1233
SstI	GAGCTC	1 cut(s)	1262
StuI	AGGCCT	1 cut(s)	601
StyD4I	CCNGG	1 cut(s)	158
StyI	CCWWGG	2 cut(s)	72, 1167
TaaI	ACNGT	1 cut(s)	682
TaiI	ACGT	2 cut(s)	105, 1313
TaqI	TCGA	6 cut(s)	117, 206, 225, 416, 583, 1273
TasI	AATT	6 cut(s)	238, 330, 477, 623, 671, 1195
TatI	WGTACW	1 cut(s)	444
TfiI	GAWTC	5 cut(s)	13, 757, 797, 881, 1298
Tru1I	TTAA	1 cut(s)	150
Tru9I	TTAA	1 cut(s)	150
TscAI	CASTG	2 cut(s)	555, 685
TseFI	GTSAC	3 cut(s)	103, 990, 1037
TseI	GCWGC	2 cut(s)	326, 1304
Tsp45I	GTSAC	3 cut(s)	103, 990, 1037
TspDTI	ATGAA	9 cut(s)	26, 360, 514, 519, 617, 770, 801, 924, 993
TspGWI	ACGGA	3 cut(s)	904, 916, 1009
TspRI	CASTG	2 cut(s)	555, 685
Van91I	CCANNNNNTGG	1 cut(s)	1168
VpaK11BI	GGWCC	4 cut(s)	980, 1059, 1108, 1134
XapI	RAATTY	3 cut(s)	623, 671, 1195
XceI	RCATGY	2 cut(s)	714, 1210
XcmI	CCANNNNNNNNNTGG	2 cut(s)	79, 626
XspI	CTAG	2 cut(s)	1029, 1233
Zsp2I	ATGCAT	1 cut(s)	1243

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.