Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008068

Physical Location & Seq

Genomic Coordinates Forward (+)

50816289 .. 50821915

Gene Structure 5627 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1408100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGGGGAGATTGATGAGCACTATGAATCTGGCGGTGGTTCTGGTGGCGGTGGCGGTGGTTTTGTTCGGCCGATTTTGCATTACCGACGCCAATGTGGTGCTGATCGGGAACAACGTTACTCTCTCTTTCGATGACTTGGAGGCTAGTTTCGCTCCGGCGGTTAAGGGTTCGGGGGAATGCGGAGTATTGTATATGGCAGAGCCTCCTGATGCGTGCTCGACATTGACTAATCAAGTCGAACAAGGCACGAATCGTAGCTCCCCTTTTGTTTTGATCGTGAGAGGAGGAGGGTGTAGTTTTGAGGATAAAGTTAGAAGAGCTCAAAAGGCCGGCTTCAAAGCTGCAATTATCTACGACAATGAAGTTAGCAGCGTGTTGGTTTCAATGGCAGGAAATTCAGCTGGTATAAAAATATACGCTGTGTTCGTTTCGAAAGCTTCTGGTGACCTACTAAAAAAGTACGCTGGACTTACTAACATGGAGCTGTGGCTAATTGCATGCTTTGAGAATTCAGCGTGGTCAATCATGGCAATCTCTTTCATTTCATTGCTTGCCATGTCTGCGGTGCTGGCTACTTGTTTTTTCGTTCGTAGGCATCGAATAAGACGAGAAAGGCCTCAAGCTTCTCGTGTTCGAGAATTTCATGGGATGAGCAAGCGCTTAGTAAAAGCAATGCCAAGTTTGATATTTACCGCAGTTTTAGAGGACAACTGTACATCAAGAACATGTGCTATATGTCTTGAGGACTATAGTGTTGGAGAAAAACTCAGGATTCTGCCATGTCGTCATAAATTTCATGCTTTCTGTGTGGATTCCTGGCTTACTTCATGGAGAACCTTTTGTCCAGTTTGCAAGCGTGATGCAAGAGCGAACACAGGTGATCTACCCGCCTCAGAATCCACACCATTGCTTTCATCCAGTCCATCCTCTGTGGCATCTTCTGTACTTTCATCTATGAGATCATCAATAGCATCATCTTCAGCAATACAGATAGGACCAGCATTGTCACGGAACCCTTCACTTTCTCATTCTCACTCTTATGCTAGCACAAGTCACATTCAGCAATCTCTTAGGTCCTCCTATCGCCAGTCCCCTTCTATTAGTGCAAGCAGAAGTTCAATGGACCTCCGAAATGTCTCGTCCCAAAGGTCTCATGCTTCCTATTTTATATCACCCAATTCCTTTGGTTACCCATCTTTATTACCCCGGAATTCAAGATACATTTCTGTTGGGCACTTTCCAAGCCCTAGCAATGCATCACCAAGCTACATCAGTTCTTCCAGTCATCGACAGCATCCACTACATTGTAGTGAATCAGCTGCAACGTTTTCTCCTTTTTCTTCTGCTCAATCTCTTCCTGAGTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000209 GO:0000322 GO:0000325 GO:0000326 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0031090 GO:0031984 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0070647 GO:0071704 GO:0098588 GO:0098791 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA zf-RING_2

Protein Analysis

456

Amino Acids

49.32

Weight (kDa)

9.3

Isoelectric Point (pI)

67.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	66 - 151	3.8e-12	PA domain
zf-RING_2	PF13639	242 - 285	2.1e-12	Ring finger domain
zf-RING_11	PF17123	243 - 270	8.1e-10	RING-like zinc finger
zf-C3HC4	PF00097	243 - 284	4.9e-08	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71980
fragaria_vesca	FvH4_5g30950 FvH4_5g30950 FvH4_5g30950 FvH4_5g30950 FvH4_5g30950
malus_domestica	MD08G1219500.v1.1 MD15G1408100.v1.1
prunus_persica	Prupe.1G553100_v2.0.a1
pyrus_communis	pycom08g19050 pycom15g36420 pycom15g36430
rosa_chinensis	RchiOBHm_Chr7g0226311
rosa_laevigata	RLG00000001752
rosa_multiflora	Rmu_sc0004322.1_g000023
rosa_roxburghii	Rroxscaffold_3G00233040
rosa_rugosa	Rorug07G0236300 Rorug07G0236400 Rorug07G0236500
rosa_samantha	Rh7AG383500 Rh7BG370300 Rh7CG403100 Rh7DG381900
rosa_wichuraiana	Rw7G032120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	8 cut(s)	32, 47, 53, 158, 180, 563, 693, 888
AclI	AACGTT	2 cut(s)	114, 1325
AcoI	YGGCCR	1 cut(s)	67
AcsI	RAATTY	5 cut(s)	394, 508, 638, 791, 1210
AcuI	CTGAAG	1 cut(s)	963
AcyI	GRCGYC	1 cut(s)	87
AfaI	GTAC	3 cut(s)	461, 715, 945
AfeI	AGCGCT	1 cut(s)	659
AflIII	ACRYGT	1 cut(s)	725
AgsI	TTSAA	4 cut(s)	337, 384, 1119, 1215
AjnI	CCWGG	1 cut(s)	815
AloI	GAACNNNNNNTCC	2 cut(s)	826, 858
AluBI	AGCT	9 cut(s)	258, 320, 341, 401, 437, 484, 623, 1266, 1319
AluI	AGCT	9 cut(s)	258, 320, 341, 401, 437, 484, 623, 1266, 1319
Alw21I	GWGCWC	3 cut(s)	20, 218, 322
Alw26I	GTCTC	2 cut(s)	1141, 1155
Aor51HI	AGCGCT	1 cut(s)	659
AoxI	GGCC	3 cut(s)	67, 327, 614
ApeKI	GCWGC	3 cut(s)	341, 369, 1319
ApoI	RAATTY	5 cut(s)	394, 508, 638, 791, 1210
AspLEI	GCGC	1 cut(s)	660
AspS9I	GGNCC	3 cut(s)	995, 1074, 1123
AsuC2I	CCSGG	1 cut(s)	1207
AsuHPI	GGTGA	4 cut(s)	455, 890, 1164, 1251
AsuII	TTCGAA	1 cut(s)	431
AsuNHI	GCTAGC	1 cut(s)	1043
AvaII	GGWCC	3 cut(s)	995, 1074, 1123
BaeGI	GKGCMC	1 cut(s)	1236
BanII	GRGCYC	1 cut(s)	322
BauI	CACGAG	1 cut(s)	627
Bbv12I	GWGCWC	3 cut(s)	20, 218, 322
BbvI	GCAGC	3 cut(s)	328, 381, 1306
BccI	CCATC	2 cut(s)	931, 1201
BciT130I	CCWGG	1 cut(s)	817
BcnI	CCSGG	1 cut(s)	1207
BcoDI	GTCTC	2 cut(s)	1141, 1155
BfaI	CTAG	3 cut(s)	144, 1044, 1248
BfmI	CTRYAG	1 cut(s)	748
BfoI	RGCGCY	1 cut(s)	661
BisI	GCNGC	3 cut(s)	342, 370, 1320
BlsI	GCNGC	3 cut(s)	343, 371, 1321
Bme1390I	CCNGG	2 cut(s)	817, 1207
Bme18I	GGWCC	3 cut(s)	995, 1074, 1123
BmgT120I	GGNCC	3 cut(s)	995, 1074, 1123
BmiI	GGNNCC	1 cut(s)	1013
BmrFI	CCNGG	2 cut(s)	817, 1207
BmsI	GCATC	7 cut(s)	199, 604, 850, 944, 980, 1265, 1303
BmtI	GCTAGC	1 cut(s)	1047
BplI	GAGNNNNNCTC	2 cut(s)	750, 782
Bpu14I	TTCGAA	1 cut(s)	431
BpuEI	CTTGAG	2 cut(s)	603, 761
BpuMI	CCSGG	1 cut(s)	1207
BsaHI	GRCGYC	1 cut(s)	87
BsaI	GGTCTC	1 cut(s)	1155
BsaJI	CCNNGG	1 cut(s)	1205
BsaXI	ACNNNNNCTCC	2 cut(s)	1315, 1345
Bse118I	RCCGGY	1 cut(s)	329
Bse1I	ACTGG	4 cut(s)	845, 918, 1087, 1281
Bse3DI	GCAATG	4 cut(s)	545, 678, 905, 1258
BseBI	CCWGG	1 cut(s)	817
BseDI	CCNNGG	1 cut(s)	1205
BseGI	GGATG	4 cut(s)	654, 914, 923, 1294
BseMI	GCAATG	4 cut(s)	545, 678, 905, 1258
BseMII	CTCAG	3 cut(s)	781, 906, 1350
BseNI	ACTGG	4 cut(s)	845, 918, 1087, 1281
BseRI	GAGGAG	2 cut(s)	297, 300
BseSI	GKGCMC	1 cut(s)	1236
BseX3I	CGGCCG	1 cut(s)	67
BseXI	GCAGC	3 cut(s)	328, 381, 1306
Bsh1285I	CGRYCG	1 cut(s)	70
BshFI	GGCC	3 cut(s)	69, 329, 616
BsiEI	CGRYCG	1 cut(s)	70
BsiHKAI	GWGCWC	3 cut(s)	20, 218, 322
BsiSI	CCGG	3 cut(s)	155, 330, 1207
BslFI	GGGAC	2 cut(s)	1075, 1126
BsmAI	GTCTC	2 cut(s)	1141, 1155
BsmFI	GGGAC	2 cut(s)	1075, 1126
BsmI	GAATGC	1 cut(s)	182
BsnI	GGCC	3 cut(s)	69, 329, 616
Bso31I	GGTCTC	1 cut(s)	1155
Bsp119I	TTCGAA	1 cut(s)	431
Bsp1286I	GDGCHC	4 cut(s)	20, 218, 322, 1236
Bsp1407I	TGTACA	1 cut(s)	713
Bsp143I	GATC	4 cut(s)	102, 273, 880, 959
BspACI	CCGC	8 cut(s)	32, 47, 53, 158, 180, 563, 693, 888
BspANI	GGCC	3 cut(s)	69, 329, 616
BspCNI	CTCAG	3 cut(s)	780, 905, 1351
BspLI	GGNNCC	1 cut(s)	1013
BspOI	GCTAGC	1 cut(s)	1047
BspQI	GCTCTTC	1 cut(s)	310
BspT104I	TTCGAA	1 cut(s)	431
BspTNI	GGTCTC	1 cut(s)	1155
BsrDI	GCAATG	4 cut(s)	545, 678, 905, 1258
BsrFI	RCCGGY	1 cut(s)	329
BsrGI	TGTACA	1 cut(s)	713
BsrI	ACTGG	4 cut(s)	845, 918, 1087, 1281
BssAI	RCCGGY	1 cut(s)	329
BssECI	CCNNGG	1 cut(s)	1205
BssMI	GATC	4 cut(s)	102, 273, 880, 959
BssNI	GRCGYC	1 cut(s)	87
BssSI	CACGAG	1 cut(s)	627
Bst2BI	CACGAG	1 cut(s)	627
Bst2UI	CCWGG	1 cut(s)	817
Bst4CI	ACNGT	1 cut(s)	713
Bst6I	CTCTTC	2 cut(s)	310, 1359
BstACI	GRCGYC	1 cut(s)	87
BstAUI	TGTACA	1 cut(s)	713
BstBI	TTCGAA	1 cut(s)	431
BstC8I	GCNNGC	9 cut(s)	214, 331, 499, 552, 570, 656, 854, 1045, 1108
BstDEI	CTNAG	5 cut(s)	661, 767, 892, 1070, 1359
BstEII	GGTNACC	2 cut(s)	443, 1187
BstF5I	GGATG	4 cut(s)	654, 914, 923, 1294
BstH2I	RGCGCY	1 cut(s)	661
BstHHI	GCGC	1 cut(s)	660
BstKTI	GATC	4 cut(s)	105, 276, 883, 962
BstMAI	GTCTC	2 cut(s)	1141, 1155
BstMBI	GATC	4 cut(s)	102, 273, 880, 959
BstMCI	CGRYCG	1 cut(s)	70
BstMWI	GCNNNNNNNGC	5 cut(s)	75, 149, 326, 560, 569
BstNI	CCWGG	1 cut(s)	817
BstNSI	RCATGY	2 cut(s)	501, 729
BstPI	GGTNACC	2 cut(s)	443, 1187
BstSCI	CCNGG	2 cut(s)	815, 1205
BstSFI	CTRYAG	1 cut(s)	748
BstSLI	GKGCMC	1 cut(s)	1236
BstV1I	GCAGC	3 cut(s)	328, 381, 1306
BstZI	CGGCCG	1 cut(s)	67
BsuRI	GGCC	3 cut(s)	69, 329, 616
BtsCI	GGATG	4 cut(s)	654, 914, 923, 1294
Cac8I	GCNNGC	9 cut(s)	214, 331, 499, 552, 570, 656, 854, 1045, 1108
CfoI	GCGC	1 cut(s)	660
Cfr10I	RCCGGY	1 cut(s)	329
Cfr13I	GGNCC	3 cut(s)	995, 1074, 1123
CseI	GACGC	1 cut(s)	95
Csp6I	GTAC	3 cut(s)	460, 714, 944
CviQI	GTAC	3 cut(s)	460, 714, 944
DdeI	CTNAG	5 cut(s)	661, 767, 892, 1070, 1359
DpnI	GATC	4 cut(s)	104, 275, 882, 961
DpnII	GATC	4 cut(s)	102, 273, 880, 959
EaeI	YGGCCR	1 cut(s)	67
EagI	CGGCCG	1 cut(s)	67
Eam1104I	CTCTTC	2 cut(s)	310, 1359
EarI	CTCTTC	2 cut(s)	310, 1359
Ecl136II	GAGCTC	1 cut(s)	320
EclXI	CGGCCG	1 cut(s)	67
Eco147I	AGGCCT	1 cut(s)	616
Eco24I	GRGCYC	1 cut(s)	322
Eco31I	GGTCTC	1 cut(s)	1155
Eco47I	GGWCC	3 cut(s)	995, 1074, 1123
Eco47III	AGCGCT	1 cut(s)	659
Eco52I	CGGCCG	1 cut(s)	67
Eco53kI	GAGCTC	1 cut(s)	320
Eco57I	CTGAAG	1 cut(s)	963
Eco91I	GGTNACC	2 cut(s)	443, 1187
EcoICRI	GAGCTC	1 cut(s)	320
EcoO109I	RGGNCCY	1 cut(s)	1074
EcoO65I	GGTNACC	2 cut(s)	443, 1187
EcoRI	GAATTC	2 cut(s)	508, 1210
EcoRII	CCWGG	1 cut(s)	815
EcoT22I	ATGCAT	1 cut(s)	1258
EcoT38I	GRGCYC	1 cut(s)	322
FalI	AAGNNNNNCTT	2 cut(s)	317, 349
FaqI	GGGAC	2 cut(s)	1075, 1126
FauI	CCCGC	1 cut(s)	895
Fnu4HI	GCNGC	3 cut(s)	342, 370, 1320
FokI	GGATG	4 cut(s)	661, 901, 910, 1281
FriOI	GRGCYC	1 cut(s)	322
Fsp4HI	GCNGC	3 cut(s)	342, 370, 1320
FspBI	CTAG	3 cut(s)	144, 1044, 1248
GlaI	GCGC	1 cut(s)	659
GluI	GCNGC	3 cut(s)	342, 370, 1320
HaeII	RGCGCY	1 cut(s)	661
HaeIII	GGCC	3 cut(s)	69, 329, 616
HapII	CCGG	3 cut(s)	155, 330, 1207
HgaI	GACGC	1 cut(s)	95
HhaI	GCGC	1 cut(s)	660
Hin1I	GRCGYC	1 cut(s)	87
Hin6I	GCGC	1 cut(s)	658
HinP1I	GCGC	1 cut(s)	658
HindIII	AAGCTT	2 cut(s)	435, 621
HinfI	GANTC	6 cut(s)	25, 250, 772, 812, 896, 1313
HpaII	CCGG	3 cut(s)	155, 330, 1207
HphI	GGTGA	4 cut(s)	455, 890, 1164, 1251
Hpy188I	TCNGA	2 cut(s)	895, 1130
Hpy188III	TCNNGA	9 cut(s)	106, 206, 277, 635, 720, 740, 769, 1215, 1358
Hpy99I	CGWCG	1 cut(s)	89
HpyAV	CCTTC	2 cut(s)	1026, 1104
HpyCH4III	ACNGT	1 cut(s)	713
HpyCH4IV	ACGT	2 cut(s)	114, 1325
HpyCH4V	TGCA	8 cut(s)	78, 344, 497, 852, 863, 1106, 1256, 1322
HpyF10VI	GCNNNNNNNGC	5 cut(s)	75, 149, 326, 560, 569
HpyF3I	CTNAG	5 cut(s)	661, 767, 892, 1070, 1359
HpySE526I	ACGT	2 cut(s)	114, 1325
Hsp92I	GRCGYC	1 cut(s)	87
HspAI	GCGC	1 cut(s)	658
KroI	GCCGGC	1 cut(s)	329
KroNI	GCCGGC	1 cut(s)	331
Kzo9I	GATC	4 cut(s)	102, 273, 880, 959
LguI	GCTCTTC	1 cut(s)	310
LmnI	GCTCC	3 cut(s)	157, 263, 481
Lsp1109I	GCAGC	3 cut(s)	328, 381, 1306
LweI	GCATC	7 cut(s)	199, 604, 850, 944, 980, 1265, 1303
MaeI	CTAG	3 cut(s)	144, 1044, 1248
MaeII	ACGT	2 cut(s)	114, 1325
MaeIII	GTNAC	5 cut(s)	115, 443, 1005, 1052, 1187
MalI	GATC	4 cut(s)	104, 275, 882, 961
MboI	GATC	4 cut(s)	102, 273, 880, 959
MboII	GAAGA	6 cut(s)	327, 930, 969, 1269, 1332, 1346
MhlI	GDGCHC	4 cut(s)	20, 218, 322, 1236
MluCI	AATT	8 cut(s)	345, 394, 492, 508, 638, 791, 1177, 1210
MmeI	TCCRAC	1 cut(s)	736
Mph1103I	ATGCAT	1 cut(s)	1258
MroNI	GCCGGC	1 cut(s)	329
MseI	TTAA	1 cut(s)	162
MslI	CAYNNNNRTG	2 cut(s)	1038, 1308
MspA1I	CMGCKG	2 cut(s)	401, 1319
MspI	CCGG	3 cut(s)	155, 330, 1207
MspR9I	CCNGG	2 cut(s)	817, 1207
Mva1269I	GAATGC	1 cut(s)	182
MvaI	CCWGG	1 cut(s)	817
MwoI	GCNNNNNNNGC	5 cut(s)	75, 149, 326, 560, 569
NaeI	GCCGGC	1 cut(s)	331
NciI	CCSGG	1 cut(s)	1207
NdeII	GATC	4 cut(s)	102, 273, 880, 959
NgoMIV	GCCGGC	1 cut(s)	329
NheI	GCTAGC	1 cut(s)	1043
NlaIV	GGNNCC	1 cut(s)	1013
NmuCI	GTSAC	3 cut(s)	443, 1005, 1052
NsiI	ATGCAT	1 cut(s)	1258
NspI	RCATGY	2 cut(s)	501, 729
NspV	TTCGAA	1 cut(s)	431
PaeI	GCATGC	1 cut(s)	501
PceI	AGGCCT	1 cut(s)	616
PciI	ACATGT	1 cut(s)	725
PciSI	GCTCTTC	1 cut(s)	310
PcsI	WCGNNNNNNNCGW	4 cut(s)	111, 423, 595, 604
PctI	GAATGC	1 cut(s)	182
PdiI	GCCGGC	1 cut(s)	331
PfeI	GAWTC	6 cut(s)	25, 250, 772, 812, 896, 1313
PkrI	GCNGC	3 cut(s)	343, 371, 1321
PpuMI	RGGWCCY	1 cut(s)	1074
PscI	ACATGT	1 cut(s)	725
Psp124BI	GAGCTC	1 cut(s)	322
Psp1406I	AACGTT	2 cut(s)	114, 1325
Psp5II	RGGWCCY	1 cut(s)	1074
Psp6I	CCWGG	1 cut(s)	815
PspEI	GGTNACC	2 cut(s)	443, 1187
PspGI	CCWGG	1 cut(s)	815
PspN4I	GGNNCC	1 cut(s)	1013
PspPI	GGNCC	3 cut(s)	995, 1074, 1123
PspPPI	RGGWCCY	1 cut(s)	1074
PvuII	CAGCTG	2 cut(s)	401, 1319
RsaI	GTAC	3 cut(s)	461, 715, 945
RsaNI	GTAC	3 cut(s)	460, 714, 944
RseI	CAYNNNNRTG	2 cut(s)	1038, 1308
SacI	GAGCTC	1 cut(s)	322
SapI	GCTCTTC	1 cut(s)	310
SaqAI	TTAA	1 cut(s)	162
SatI	GCNGC	3 cut(s)	342, 370, 1320
Sau3AI	GATC	4 cut(s)	102, 273, 880, 959
Sau96I	GGNCC	3 cut(s)	995, 1074, 1123
ScrFI	CCNGG	2 cut(s)	817, 1207
SduI	GDGCHC	4 cut(s)	20, 218, 322, 1236
SfaNI	GCATC	7 cut(s)	199, 604, 850, 944, 980, 1265, 1303
SfcI	CTRYAG	1 cut(s)	748
SfuI	TTCGAA	1 cut(s)	431
SinI	GGWCC	3 cut(s)	995, 1074, 1123
SmiMI	CAYNNNNRTG	2 cut(s)	1038, 1308
SmlI	CTYRAG	2 cut(s)	618, 740
SmoI	CTYRAG	2 cut(s)	618, 740
SphI	GCATGC	1 cut(s)	501
Sse9I	AATT	8 cut(s)	345, 394, 492, 508, 638, 791, 1177, 1210
SseBI	AGGCCT	1 cut(s)	616
SsiI	CCGC	8 cut(s)	32, 47, 53, 158, 180, 563, 693, 888
SspMI	CTAG	3 cut(s)	144, 1044, 1248
SstI	GAGCTC	1 cut(s)	322
StuI	AGGCCT	1 cut(s)	616
StyD4I	CCNGG	2 cut(s)	815, 1205
TaaI	ACNGT	1 cut(s)	713
TaiI	ACGT	2 cut(s)	117, 1328
TaqI	TCGA	7 cut(s)	129, 218, 237, 431, 598, 634, 1288
TasI	AATT	8 cut(s)	345, 394, 492, 508, 638, 791, 1177, 1210
TatI	WGTACW	2 cut(s)	713, 943
TfiI	GAWTC	6 cut(s)	25, 250, 772, 812, 896, 1313
Tru1I	TTAA	1 cut(s)	162
Tru9I	TTAA	1 cut(s)	162
TseFI	GTSAC	3 cut(s)	443, 1005, 1052
TseI	GCWGC	3 cut(s)	341, 369, 1319
Tsp45I	GTSAC	3 cut(s)	443, 1005, 1052
TspDTI	ATGAA	9 cut(s)	38, 375, 529, 534, 632, 785, 816, 903, 939
TspGWI	ACGGA	1 cut(s)	1024
VpaK11BI	GGWCC	3 cut(s)	995, 1074, 1123
XapI	RAATTY	5 cut(s)	394, 508, 638, 791, 1210
XceI	RCATGY	2 cut(s)	501, 729
XspI	CTAG	3 cut(s)	144, 1044, 1248
Zsp2I	ATGCAT	1 cut(s)	1258

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.