FvH4_5g33020

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012550
Physical Location & Seq
Forward (+)
23845552 .. 23846815
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33020.t1

Sequence Viewer

Length: 537 bp
ATGAAGAACAAGGCAGGGCTAGCGAAGGTGACGGGAACAGTAGTGTGTATAAGTGGAGCAATGATACTGGCATTTTACCATGGGACAATTATTGAGATAGGTGAATCCAAAATTCACTGGAGCTATGCTCAGAAATTGAGAGAGTCAAGTGCTGCTGGCAACAATGCCAACTCCTTTGCCAAAGTGAGTGTTATGTTTCCGGCTCCTTACACAAGCACTGCATTGATGTGTTTCATGGCCAGTTTGGAGTGTGGGCTCATTGCTGGGATTATGGGTTCTGCAGTAGCATTTTTCCTCACTTTATGGTGTATTCAGAGGAAAGGACCTCCATATGTGTCAGTCTTCAACCCTCTGCTGCTCATTTTTGTGGCTGTTTCGAGTTGGGCTCTGCTTCATGAGAAAATGTACCTGGGAACTGCGATAGGGTCAATCTTAATCGTGGTCGGGCTCTATACTGTTCTTTGGGGGAAGAGAAAGGAGAAGGCGAAGCTTGAAACCAACCCAAATGCTAACTTGGAGCTGCAGCTGCCCAAATAG

Protein Analysis

179

Amino Acids

19.29

Weight (kDa)

9.44

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 84 - 154 1.3e-08 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012550)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09380
fragaria_vesca FvH4_5g33020 FvH4_6g08060 FvH4_6g08060
malus_domestica MD04G1189900.v1.1 MD04G1190500.v1.1 MD12G1202700.v1.1
prunus_persica Prupe.6G308500_v2.0.a1
pyrus_communis pycom04g16830 pycom12g18920
rosa_chinensis RchiOBHm_Chr3g0457731
rosa_laevigata RLG00000025190
rosa_roxburghii Rroxscaffold_6G00421530
rosa_rugosa Rorug03G0023000 Rorug03G0023100
rosa_samantha Rh3AG083700 Rh3CG086400 Rh3DG087100
rosa_wichuraiana Rw3G007170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 1 cut(s) 111
AfaI GTAC 1 cut(s) 407
AgsI TTSAA 2 cut(s) 346, 494
AjnI CCWGG 1 cut(s) 408
AloI GAACNNNNNNTCC 2 cut(s) 259, 291
AluBI AGCT 4 cut(s) 123, 490, 520, 526
AluI AGCT 4 cut(s) 123, 490, 520, 526
AoxI GGCC 1 cut(s) 237
ApeKI GCWGC 5 cut(s) 152, 355, 520, 523, 526
ApoI RAATTY 1 cut(s) 111
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 2 cut(s) 40, 113
AsuNHI GCTAGC 1 cut(s) 19
AvaII GGWCC 1 cut(s) 323
BalI TGGCCA 1 cut(s) 239
BanII GRGCYC 3 cut(s) 258, 388, 450
BbsI GAAGAC 1 cut(s) 334
BbvI GCAGC 4 cut(s) 139, 342, 507, 513
BciT130I CCWGG 1 cut(s) 410
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 2 cut(s) 279, 521
BisI GCNGC 5 cut(s) 153, 356, 521, 524, 527
BlsI GCNGC 5 cut(s) 154, 357, 522, 525, 528
Bme1390I CCNGG 1 cut(s) 410
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 410
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 334
BplI GAGNNNNNCTC 4 cut(s) 112, 144, 370, 402
BpmI CTGGAG 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 79, 409
Bse1I ACTGG 3 cut(s) 72, 122, 240
Bse3DI GCAATG 2 cut(s) 66, 258
BseBI CCWGG 1 cut(s) 410
BseDI CCNNGG 2 cut(s) 79, 409
BseMI GCAATG 2 cut(s) 66, 258
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 3 cut(s) 72, 122, 240
BseXI GCAGC 4 cut(s) 139, 342, 507, 513
BseYI CCCAGC 1 cut(s) 263
BshFI GGCC 1 cut(s) 239
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 1 cut(s) 239
Bsp1286I GDGCHC 3 cut(s) 258, 388, 450
Bsp19I CCATGG 1 cut(s) 79
BspANI GGCC 1 cut(s) 239
BspCNI CTCAG 1 cut(s) 142
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 204
BspMAI CTGCAG 2 cut(s) 283, 525
BspOI GCTAGC 1 cut(s) 23
BsrDI GCAATG 2 cut(s) 66, 258
BsrI ACTGG 3 cut(s) 72, 122, 240
BssECI CCNNGG 2 cut(s) 79, 409
BssT1I CCWWGG 1 cut(s) 79
Bst2UI CCWGG 1 cut(s) 410
Bst4CI ACNGT 2 cut(s) 40, 457
Bst6I CTCTTC 1 cut(s) 464
BstC8I GCNNGC 2 cut(s) 21, 157
BstDEI CTNAG 1 cut(s) 129
BstDSI CCRYGG 1 cut(s) 79
BstMWI GCNNNNNNNGC 2 cut(s) 20, 526
BstNI CCWGG 1 cut(s) 410
BstSCI CCNGG 1 cut(s) 408
BstSFI CTRYAG 2 cut(s) 279, 521
BstV1I GCAGC 4 cut(s) 139, 342, 507, 513
BstV2I GAAGAC 1 cut(s) 334
BsuRI GGCC 1 cut(s) 239
BtgI CCRYGG 1 cut(s) 79
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 115, 216
Cac8I GCNNGC 2 cut(s) 21, 157
CciI TCATGA 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 406
CviAII CATG 3 cut(s) 80, 235, 395
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 1 cut(s) 129
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 464
EarI CTCTTC 1 cut(s) 464
Eco130I CCWWGG 1 cut(s) 79
Eco24I GRGCYC 3 cut(s) 258, 388, 450
Eco47I GGWCC 1 cut(s) 323
EcoO109I RGGNCCY 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 79
EcoT38I GRGCYC 3 cut(s) 258, 388, 450
ErhI CCWWGG 1 cut(s) 79
FaeI CATG 3 cut(s) 83, 238, 398
FaqI GGGAC 1 cut(s) 97
FatI CATG 3 cut(s) 79, 234, 394
FauNDI CATATG 1 cut(s) 331
Fnu4HI GCNGC 5 cut(s) 153, 356, 521, 524, 527
FriOI GRGCYC 3 cut(s) 258, 388, 450
Fsp4HI GCNGC 5 cut(s) 153, 356, 521, 524, 527
FspBI CTAG 1 cut(s) 20
GluI GCNGC 5 cut(s) 153, 356, 521, 524, 527
GsaI CCCAGC 1 cut(s) 267
GsuI CTGGAG 1 cut(s) 139
HaeIII GGCC 1 cut(s) 239
HapII CCGG 1 cut(s) 200
Hin1II CATG 3 cut(s) 83, 238, 398
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 2 cut(s) 104, 143
HpaII CCGG 1 cut(s) 200
HphI GGTGA 2 cut(s) 40, 113
Hpy188I TCNGA 2 cut(s) 132, 315
Hpy188III TCNNGA 1 cut(s) 395
HpyAV CCTTC 2 cut(s) 19, 475
HpyCH4III ACNGT 2 cut(s) 40, 457
HpyCH4V TGCA 3 cut(s) 221, 281, 523
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 526
HpyF3I CTNAG 1 cut(s) 129
Hsp92II CATG 3 cut(s) 83, 238, 398
LmnI GCTCC 4 cut(s) 56, 120, 208, 517
LpnPI CCDG 8 cut(s) 53, 103, 141, 213, 249, 253, 395, 422
Lsp1109I GCAGC 4 cut(s) 139, 342, 507, 513
MaeI CTAG 1 cut(s) 20
MaeIII GTNAC 1 cut(s) 28
MboII GAAGA 3 cut(s) 16, 334, 481
MhlI GDGCHC 3 cut(s) 258, 388, 450
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 3 cut(s) 87, 111, 134
MluNI TGGCCA 1 cut(s) 239
MlyI GAGTC 1 cut(s) 152
MnlI CCTC 4 cut(s) 305, 309, 336, 360
Mox20I TGGCCA 1 cut(s) 239
MscI TGGCCA 1 cut(s) 239
MseI TTAA 1 cut(s) 434
MslI CAYNNNNRTG 2 cut(s) 226, 365
Msp20I TGGCCA 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 526
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 410
MvaI CCWGG 1 cut(s) 410
MwoI GCNNNNNNNGC 2 cut(s) 20, 526
NcoI CCATGG 1 cut(s) 79
NdeI CATATG 1 cut(s) 331
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 3 cut(s) 83, 238, 398
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 28
PagI TCATGA 1 cut(s) 394
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 5 cut(s) 154, 357, 522, 525, 528
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
PpuMI RGGWCCY 1 cut(s) 323
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 408
PspFI CCCAGC 1 cut(s) 263
PspGI CCWGG 1 cut(s) 408
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 323
PspPPI RGGWCCY 1 cut(s) 323
PstI CTGCAG 2 cut(s) 283, 525
PvuII CAGCTG 1 cut(s) 526
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
RseI CAYNNNNRTG 2 cut(s) 226, 365
SaqAI TTAA 1 cut(s) 434
SatI GCNGC 5 cut(s) 153, 356, 521, 524, 527
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 1 cut(s) 410
SduI GDGCHC 3 cut(s) 258, 388, 450
SetI ASST 8 cut(s) 30, 103, 125, 328, 411, 492, 522, 528
SfcI CTRYAG 2 cut(s) 279, 521
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 2 cut(s) 226, 365
Sse9I AATT 3 cut(s) 87, 111, 134
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 1 cut(s) 408
StyI CCWWGG 1 cut(s) 79
TaaI ACNGT 2 cut(s) 40, 457
TaqI TCGA 1 cut(s) 377
TasI AATT 3 cut(s) 87, 111, 134
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TscAI CASTG 2 cut(s) 122, 223
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 5 cut(s) 152, 355, 520, 523, 526
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 3 cut(s) 17, 223, 383
TspRI CASTG 2 cut(s) 122, 223
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 111
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.