Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0012550

Physical Location & Seq

Genomic Coordinates Forward (+)

28008481 .. 28011702

Gene Structure 3222 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1189900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGGCTGCCTTTAGCGCCTACATGCCATTCATGGCTATGGTGTTGGTCCAATCGAGCTATGCAGGAATGAGGATTATTTCAAAGCTTGCTATTGAATCTGACATGGACCCTCTTATTCTTGTTGCTTATCGTCAAGTTTTCGCGGCCGTTGCCATTGCTCCCTTAGCTTATTTGTTGGAATGGAAAACAAGACCCAAGATCACATTGCCTATTCTTTTCCAGACTTTTTTGTGCTCATTAACTGGGGCCACTGCAAATCAGGTTTTTTATTTTCTCGGTTTAAAGTCTTCGACCGCAACGATTGCATGCGCATTGACAAACACACTTCCAGCCATGGCGTTTATCCTTGCAGTCATTTTCAGCAAAGCAGGGCTATCTAAGGTGATGGGAACAATTGTGTGTGTATCTGGAGCCATGTTACTTTCATTTTACCATGGACACATCATTGGTCTAGGTGAGTCCAAAATTCACTGGACATATGCTCAGAGAATGGGAGAGACAAGTTCCAGCACCAAATTAAGCTCCTTTATAGGCCCCCTATTTGTCATCTTAAGCACCTTAGGTTGGGCATTCTGGTTCATAATCCAAGCTAGAGTTGGGGAAAGCTTTCCAGCTCCTTATACAAGCACCACATTGATGTGTTTGATGGCCAGCTTTGAGTGCGGGATCATTGCTGTAATTGCTGATCACAAAGTGGCTTCATGGTGGCTAAAAGATCCCACGAGGCTTATTGCATCCCTCTATGCTGGAATTATGGGTTCTGCACTAGCATTCTTCCTCTCTTCGTGGAGTATTCAGCGAAAAGGCCCTCTTTACGTGTCGGTGTTTAGGTCGGTTTTGATTGTTGCCGGGCTCTATCTTGTTCTGTGGGGGAAGAATAAGGAGACGAAGGATGAGAAGCCAACAAAAGCAACAGGCATGACAAATATGACAGATCAGGCCAAGCCTTATGAGAAAAACGACTTGGAGCTGCAGCTGCAAGGGCATTCGGAAAGCAACAGTGACGTTATGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0006810 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009987 GO:0010033 GO:0016020 GO:0016021 GO:0022857 GO:0023052 GO:0031224 GO:0031226 GO:0032870 GO:0042221 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

339

Amino Acids

37.07

Weight (kDa)

8.97

Isoelectric Point (pI)

34.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	12 - 142	9.4e-15	EamA-like transporter family
EamA	PF00892	177 - 283	1.1e-08	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012550)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09380
fragaria_vesca	FvH4_5g33020 FvH4_6g08060 FvH4_6g08060
malus_domestica	MD04G1189900.v1.1 MD04G1190500.v1.1 MD12G1202700.v1.1
prunus_persica	Prupe.6G308500_v2.0.a1
pyrus_communis	pycom04g16830 pycom12g18920
rosa_chinensis	RchiOBHm_Chr3g0457731
rosa_laevigata	RLG00000025190
rosa_roxburghii	Rroxscaffold_6G00421530
rosa_rugosa	Rorug03G0023000 Rorug03G0023100
rosa_samantha	Rh3AG083700 Rh3CG086400 Rh3DG087100
rosa_wichuraiana	Rw3G007170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	310
AccII	CGCG	1 cut(s)	143
AciI	CCGC	3 cut(s)	143, 294, 663
AclWI	GGATC	2 cut(s)	674, 710
AcoI	YGGCCR	2 cut(s)	144, 648
AcsI	RAATTY	1 cut(s)	465
AdeI	CACNNNGTG	1 cut(s)	694
AfiI	CCNNNNNNNGG	1 cut(s)	564
AflII	CTTAAG	1 cut(s)	550
AflIII	ACRYGT	1 cut(s)	816
AgsI	TTSAA	2 cut(s)	81, 95
AjuI	GAANNNNNNNTTGG	2 cut(s)	947, 979
Alw21I	GWGCWC	1 cut(s)	236
Alw26I	GTCTC	2 cut(s)	491, 878
AlwI	GGATC	2 cut(s)	674, 710
AoxI	GGCC	6 cut(s)	144, 246, 532, 648, 805, 939
ApeKI	GCWGC	4 cut(s)	5, 970, 973, 976
ApoI	RAATTY	1 cut(s)	465
Asp700I	GAANNNNTTC	1 cut(s)	606
AspLEI	GCGC	2 cut(s)	17, 311
AspS9I	GGNCC	5 cut(s)	46, 106, 246, 533, 806
AsuC2I	CCSGG	1 cut(s)	850
AsuHPI	GGTGA	2 cut(s)	394, 467
AvaII	GGWCC	2 cut(s)	46, 106
AxyI	CCTNAGG	1 cut(s)	559
BalI	TGGCCA	1 cut(s)	650
BanII	GRGCYC	1 cut(s)	855
BauI	CACGAG	1 cut(s)	721
BbsI	GAAGAC	1 cut(s)	279
Bbv12I	GWGCWC	1 cut(s)	236
BbvI	GCAGC	3 cut(s)	957, 963, 985
BccI	CCATC	2 cut(s)	379, 640
BceAI	ACGGC	1 cut(s)	131
BclI	TGATCA	1 cut(s)	685
BcnI	CCSGG	1 cut(s)	850
BcoDI	GTCTC	2 cut(s)	491, 878
BfaI	CTAG	3 cut(s)	452, 591, 767
BfmI	CTRYAG	1 cut(s)	971
BfoI	RGCGCY	1 cut(s)	18
BfrI	CTTAAG	1 cut(s)	550
BisI	GCNGC	5 cut(s)	6, 144, 971, 974, 977
BlsI	GCNGC	5 cut(s)	7, 145, 972, 975, 978
Bme1390I	CCNGG	1 cut(s)	850
Bme18I	GGWCC	2 cut(s)	46, 106
BmgT120I	GGNCC	5 cut(s)	46, 106, 246, 533, 806
BmiI	GGNNCC	4 cut(s)	108, 247, 412, 535
BmrFI	CCNGG	1 cut(s)	850
BmrI	ACTGGG	1 cut(s)	252
BmsI	GCATC	1 cut(s)	743
BmuI	ACTGGG	1 cut(s)	252
BpiI	GAAGAC	1 cut(s)	279
BpmI	CTGGAG	1 cut(s)	429
Bpu10I	CCTNAGC	1 cut(s)	163
BpuMI	CCSGG	1 cut(s)	850
BsaAI	YACGTR	1 cut(s)	817
BsaJI	CCNNGG	2 cut(s)	333, 433
Bsc4I	CCNNNNNNNGG	1 cut(s)	564
Bse1I	ACTGG	2 cut(s)	247, 476
Bse21I	CCTNAGG	1 cut(s)	559
Bse3DI	GCAATG	3 cut(s)	153, 203, 669
BseDI	CCNNGG	2 cut(s)	333, 433
BseGI	GGATG	2 cut(s)	734, 898
BseLI	CCNNNNNNNGG	1 cut(s)	564
BseMI	GCAATG	3 cut(s)	153, 203, 669
BseMII	CTCAG	1 cut(s)	497
BseNI	ACTGG	2 cut(s)	247, 476
BseX3I	CGGCCG	1 cut(s)	144
BseXI	GCAGC	3 cut(s)	957, 963, 985
BsgI	GTGCAG	1 cut(s)	747
Bsh1236I	CGCG	1 cut(s)	143
Bsh1285I	CGRYCG	2 cut(s)	147, 294
BshFI	GGCC	6 cut(s)	146, 248, 534, 650, 807, 941
BsiEI	CGRYCG	2 cut(s)	147, 294
BsiHKAI	GWGCWC	1 cut(s)	236
BsiSI	CCGG	1 cut(s)	849
BslI	CCNNNNNNNGG	1 cut(s)	564
BsmAI	GTCTC	2 cut(s)	491, 878
BsmBI	CGTCTC	1 cut(s)	878
BsmI	GAATGC	3 cut(s)	569, 770, 985
BsnI	GGCC	6 cut(s)	146, 248, 534, 650, 807, 941
Bsp1286I	GDGCHC	2 cut(s)	236, 855
Bsp143I	GATC	5 cut(s)	198, 666, 685, 715, 934
Bsp19I	CCATGG	2 cut(s)	333, 433
BspACI	CCGC	3 cut(s)	143, 294, 663
BspANI	GGCC	6 cut(s)	146, 248, 534, 650, 807, 941
BspCNI	CTCAG	1 cut(s)	496
BspFNI	CGCG	1 cut(s)	143
BspLI	GGNNCC	4 cut(s)	108, 247, 412, 535
BspMAI	CTGCAG	1 cut(s)	975
BspPI	GGATC	2 cut(s)	674, 710
BspTI	CTTAAG	1 cut(s)	550
BsrDI	GCAATG	3 cut(s)	153, 203, 669
BsrI	ACTGG	2 cut(s)	247, 476
BssECI	CCNNGG	2 cut(s)	333, 433
BssMI	GATC	5 cut(s)	198, 666, 685, 715, 934
BssSI	CACGAG	1 cut(s)	721
BssT1I	CCWWGG	2 cut(s)	333, 433
Bst2BI	CACGAG	1 cut(s)	721
Bst4CI	ACNGT	1 cut(s)	1001
Bst6I	CTCTTC	1 cut(s)	787
BstAFI	CTTAAG	1 cut(s)	550
BstAPI	GCANNNNNTGC	1 cut(s)	302
BstBAI	YACGTR	1 cut(s)	817
BstC8I	GCNNGC	3 cut(s)	87, 307, 652
BstDEI	CTNAG	4 cut(s)	163, 378, 483, 559
BstDSI	CCRYGG	2 cut(s)	333, 433
BstF5I	GGATG	2 cut(s)	734, 898
BstFNI	CGCG	1 cut(s)	143
BstH2I	RGCGCY	1 cut(s)	18
BstHHI	GCGC	2 cut(s)	17, 311
BstKTI	GATC	5 cut(s)	201, 669, 688, 718, 937
BstMAI	GTCTC	2 cut(s)	491, 878
BstMBI	GATC	5 cut(s)	198, 666, 685, 715, 934
BstMCI	CGRYCG	2 cut(s)	147, 294
BstMWI	GCNNNNNNNGC	8 cut(s)	14, 149, 164, 302, 660, 680, 976, 982
BstNSI	RCATGY	2 cut(s)	25, 309
BstSCI	CCNGG	1 cut(s)	848
BstSFI	CTRYAG	1 cut(s)	971
BstUI	CGCG	1 cut(s)	143
BstV1I	GCAGC	3 cut(s)	957, 963, 985
BstV2I	GAAGAC	1 cut(s)	279
BstX2I	RGATCY	1 cut(s)	715
BstYI	RGATCY	1 cut(s)	715
BstZI	CGGCCG	1 cut(s)	144
Bsu36I	CCTNAGG	1 cut(s)	559
BsuRI	GGCC	6 cut(s)	146, 248, 534, 650, 807, 941
BtgI	CCRYGG	2 cut(s)	333, 433
BtsCI	GGATG	2 cut(s)	734, 898
BtsI	GCAGTG	1 cut(s)	249
BtsIMutI	CAGTG	3 cut(s)	249, 469, 1006
Cac8I	GCNNGC	3 cut(s)	87, 307, 652
CfoI	GCGC	2 cut(s)	17, 311
Cfr13I	GGNCC	5 cut(s)	46, 106, 246, 533, 806
CviAII	CATG	9 cut(s)	22, 31, 103, 306, 334, 415, 434, 702, 919
DdeI	CTNAG	4 cut(s)	163, 378, 483, 559
DpnI	GATC	5 cut(s)	200, 668, 687, 717, 936
DpnII	GATC	5 cut(s)	198, 666, 685, 715, 934
DraI	TTTAAA	1 cut(s)	282
DraIII	CACNNNGTG	1 cut(s)	694
EaeI	YGGCCR	2 cut(s)	144, 648
EagI	CGGCCG	1 cut(s)	144
Eam1104I	CTCTTC	1 cut(s)	787
EarI	CTCTTC	1 cut(s)	787
EclXI	CGGCCG	1 cut(s)	144
Eco130I	CCWWGG	2 cut(s)	333, 433
Eco24I	GRGCYC	1 cut(s)	855
Eco47I	GGWCC	2 cut(s)	46, 106
Eco52I	CGGCCG	1 cut(s)	144
Eco81I	CCTNAGG	1 cut(s)	559
EcoO109I	RGGNCCY	2 cut(s)	533, 806
EcoT14I	CCWWGG	2 cut(s)	333, 433
EcoT38I	GRGCYC	1 cut(s)	855
ErhI	CCWWGG	2 cut(s)	333, 433
Esp3I	CGTCTC	1 cut(s)	878
FaeI	CATG	9 cut(s)	25, 34, 106, 309, 337, 418, 437, 705, 922
FalI	AAGNNNNNCTT	2 cut(s)	795, 827
FatI	CATG	9 cut(s)	21, 30, 102, 305, 333, 414, 433, 701, 918
FauI	CCCGC	1 cut(s)	656
FauNDI	CATATG	1 cut(s)	478
FbaI	TGATCA	1 cut(s)	685
Fnu4HI	GCNGC	5 cut(s)	6, 144, 971, 974, 977
FokI	GGATG	2 cut(s)	721, 905
FriOI	GRGCYC	1 cut(s)	855
Fsp4HI	GCNGC	5 cut(s)	6, 144, 971, 974, 977
FspAI	RTGCGCAY	1 cut(s)	310
FspBI	CTAG	3 cut(s)	452, 591, 767
FspI	TGCGCA	1 cut(s)	310
GlaI	GCGC	2 cut(s)	16, 310
GluI	GCNGC	5 cut(s)	6, 144, 971, 974, 977
GsuI	CTGGAG	1 cut(s)	429
HaeII	RGCGCY	1 cut(s)	18
HaeIII	GGCC	6 cut(s)	146, 248, 534, 650, 807, 941
HapII	CCGG	1 cut(s)	849
HhaI	GCGC	2 cut(s)	17, 311
Hin1II	CATG	9 cut(s)	25, 34, 106, 309, 337, 418, 437, 705, 922
Hin6I	GCGC	2 cut(s)	15, 309
HinP1I	GCGC	2 cut(s)	15, 309
HindIII	AAGCTT	2 cut(s)	83, 604
HinfI	GANTC	2 cut(s)	95, 458
HpaII	CCGG	1 cut(s)	849
HphI	GGTGA	2 cut(s)	394, 467
Hpy188I	TCNGA	3 cut(s)	100, 486, 991
Hpy188III	TCNNGA	2 cut(s)	220, 408
HpyAV	CCTTC	1 cut(s)	883
HpyCH4III	ACNGT	1 cut(s)	1001
HpyCH4IV	ACGT	2 cut(s)	816, 1005
HpyCH4V	TGCA	8 cut(s)	62, 254, 305, 350, 734, 764, 973, 979
HpyF10VI	GCNNNNNNNGC	8 cut(s)	14, 149, 164, 302, 660, 680, 976, 982
HpyF3I	CTNAG	4 cut(s)	163, 378, 483, 559
HpySE526I	ACGT	2 cut(s)	816, 1005
Hsp92II	CATG	9 cut(s)	25, 34, 106, 309, 337, 418, 437, 705, 922
HspAI	GCGC	2 cut(s)	15, 309
Ksp22I	TGATCA	1 cut(s)	685
Kzo9I	GATC	5 cut(s)	198, 666, 685, 715, 934
LmnI	GCTCC	5 cut(s)	163, 410, 527, 619, 967
Lsp1109I	GCAGC	3 cut(s)	957, 963, 985
LweI	GCATC	1 cut(s)	743
MaeI	CTAG	3 cut(s)	452, 591, 767
MaeII	ACGT	2 cut(s)	816, 1005
MaeIII	GTNAC	2 cut(s)	417, 1001
MalI	GATC	5 cut(s)	200, 668, 687, 717, 936
MboI	GATC	5 cut(s)	198, 666, 685, 715, 934
MboII	GAAGA	4 cut(s)	279, 766, 774, 886
MfeI	CAATTG	1 cut(s)	393
MflI	RGATCY	1 cut(s)	715
MhlI	GDGCHC	2 cut(s)	236, 855
MlsI	TGGCCA	1 cut(s)	650
MluCI	AATT	5 cut(s)	393, 465, 515, 678, 750
MluNI	TGGCCA	1 cut(s)	650
MlyI	GAGTC	1 cut(s)	467
MmeI	TCCRAC	1 cut(s)	156
MnlI	CCTC	6 cut(s)	63, 120, 717, 749, 788, 819
Mox20I	TGGCCA	1 cut(s)	650
MroXI	GAANNNNTTC	1 cut(s)	606
MscI	TGGCCA	1 cut(s)	650
MseI	TTAA	4 cut(s)	239, 281, 518, 551
MslI	CAYNNNNRTG	3 cut(s)	35, 635, 637
Msp20I	TGGCCA	1 cut(s)	650
MspA1I	CMGCKG	1 cut(s)	976
MspCI	CTTAAG	1 cut(s)	550
MspI	CCGG	1 cut(s)	849
MspR9I	CCNGG	1 cut(s)	850
MunI	CAATTG	1 cut(s)	393
Mva1269I	GAATGC	3 cut(s)	569, 770, 985
MvnI	CGCG	1 cut(s)	143
MwoI	GCNNNNNNNGC	8 cut(s)	14, 149, 164, 302, 660, 680, 976, 982
NciI	CCSGG	1 cut(s)	850
NcoI	CCATGG	2 cut(s)	333, 433
NdeI	CATATG	1 cut(s)	478
NdeII	GATC	5 cut(s)	198, 666, 685, 715, 934
NlaIII	CATG	9 cut(s)	25, 34, 106, 309, 337, 418, 437, 705, 922
NlaIV	GGNNCC	4 cut(s)	108, 247, 412, 535
NmuCI	GTSAC	1 cut(s)	1001
NsbI	TGCGCA	1 cut(s)	310
NspI	RCATGY	2 cut(s)	25, 309
PaeI	GCATGC	1 cut(s)	309
PcsI	WCGNNNNNNNCGW	1 cut(s)	296
PctI	GAATGC	3 cut(s)	569, 770, 985
PdmI	GAANNNNTTC	1 cut(s)	606
PfeI	GAWTC	1 cut(s)	95
PkrI	GCNGC	5 cut(s)	7, 145, 972, 975, 978
PleI	GAGTC	1 cut(s)	466
PpsI	GAGTC	1 cut(s)	466
Ppu21I	YACGTR	1 cut(s)	817
PspN4I	GGNNCC	4 cut(s)	108, 247, 412, 535
PspPI	GGNCC	5 cut(s)	46, 106, 246, 533, 806
PstI	CTGCAG	1 cut(s)	975
PsuI	RGATCY	1 cut(s)	715
PvuII	CAGCTG	1 cut(s)	976
RseI	CAYNNNNRTG	3 cut(s)	35, 635, 637
SaqAI	TTAA	4 cut(s)	239, 281, 518, 551
SatI	GCNGC	5 cut(s)	6, 144, 971, 974, 977
Sau3AI	GATC	5 cut(s)	198, 666, 685, 715, 934
Sau96I	GGNCC	5 cut(s)	46, 106, 246, 533, 806
SchI	GAGTC	1 cut(s)	467
ScrFI	CCNGG	1 cut(s)	850
SduI	GDGCHC	2 cut(s)	236, 855
SfaNI	GCATC	1 cut(s)	743
SfcI	CTRYAG	1 cut(s)	971
SinI	GGWCC	2 cut(s)	46, 106
SmiMI	CAYNNNNRTG	3 cut(s)	35, 635, 637
SmlI	CTYRAG	1 cut(s)	550
SmoI	CTYRAG	1 cut(s)	550
SphI	GCATGC	1 cut(s)	309
Sse9I	AATT	5 cut(s)	393, 465, 515, 678, 750
SsiI	CCGC	3 cut(s)	143, 294, 663
SspMI	CTAG	3 cut(s)	452, 591, 767
StyD4I	CCNGG	1 cut(s)	848
StyI	CCWWGG	2 cut(s)	333, 433
TaaI	ACNGT	1 cut(s)	1001
TaiI	ACGT	2 cut(s)	819, 1008
TaqI	TCGA	2 cut(s)	53, 290
TasI	AATT	5 cut(s)	393, 465, 515, 678, 750
TauI	GCSGC	1 cut(s)	146
TfiI	GAWTC	1 cut(s)	95
Tru1I	TTAA	4 cut(s)	239, 281, 518, 551
Tru9I	TTAA	4 cut(s)	239, 281, 518, 551
TscAI	CASTG	3 cut(s)	256, 476, 1006
TseFI	GTSAC	1 cut(s)	1001
TseI	GCWGC	4 cut(s)	5, 970, 973, 976
Tsp45I	GTSAC	1 cut(s)	1001
TspDTI	ATGAA	4 cut(s)	19, 414, 568, 690
TspRI	CASTG	3 cut(s)	256, 476, 1006
Vha464I	CTTAAG	1 cut(s)	550
VpaK11BI	GGWCC	2 cut(s)	46, 106
XapI	RAATTY	1 cut(s)	465
XceI	RCATGY	2 cut(s)	25, 309
XcmI	CCANNNNNNNNNTGG	1 cut(s)	593
XmnI	GAANNNNTTC	1 cut(s)	606
XspI	CTAG	3 cut(s)	452, 591, 767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.