Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012550

Physical Location & Seq

Genomic Coordinates Forward (+)

28302842 .. 28307117

Gene Structure 4276 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1202700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1116 bp

ATGGCTGGCTATAGCGAATACATGCCATTCATGGCCATGGTTTTGGTCCAAGCGAGCTATGCAGGAATGAACATTATTTCAAAGCTTGCTATTGAATCTGACATGGACCCTCTTGTTCTTGTTGCTTATCGTCAAGTTTTCGCCACCGCAGTCATTGCTCCCCTTGCTTATTTGATGGAATGGAAAACAAGACCGAAGATCACGTTGCCCATTCTTTTCCAGACTTTTCTGTGCTCATTAACTGGGGCCACTGCAAATCAGGTTTTTTATTTTCTCGGTTTAAAGACTTCGACCGCAACGATTGCATGCGCATTGCAAAACACACTTCCAGCCATGACATTTATCCTTGCAGTCATTTTCAGACAAGAATCTGCAAAGCTCAAGAGCAAAGCAGGGCTATCTAAGGCGATGGGAACAATTGTGTGTGTATCTGGAGCTATGTTACTTTCATTTTACCATGGACACATCATTGGTCTAGGTGACTCCAAAATTCACTGGACATATGCGCAGAGAATGGGAGAAGCAAGTTCCAGCACCAAATCAAGCTCCTTTATTGGCCCCCTTTTTGTCATCTTAAGCACCTTAGGTTGGGCATTCTGGTTCATAATCCAAGCTAGAGTGGGGGAAAGCTTTCCAGCTCCTTATACAAGCACCACACTGATGTGTTTGATGGCCAGCTTTGAGTGTGGGATCATTGCTGTAATTGCTGATCACAAAGCGGTTTCATGGTCTCTAAAAGATCCCATTAGGCTTGTTGCATCCCTCTTTGCTGGAATTATGGGTTCTGCACTAGCATTCTTCCTCTCTTCATGGAGTATTCAGCGAAAAGGCCCTCTTTACGTGTCGGTGTTTAGTCCCTTGCTCCTCATCATTGTTGCCATTGCAAGCTGGGCTTTGCTCGATGAGAAATTATACGTTGGAACTGCTGTAGGGTCAGTTTTGATTGTTGCCGGGCTCTATCTTGTTCTTTGGGGAAAGAATAAGGAGACGAAGGATGAGAAGCCAACAAAAGCAACAGGCATGACAAAGACGACAGATGAGGCCAAGGCTTATGAGAAAAGCGACTTGGAGCTGCAGCAAGTGCATCCGAAAAGCATCCGTGACGTTATGGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0006810 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009987 GO:0010033 GO:0016020 GO:0016021 GO:0022857 GO:0023052 GO:0031224 GO:0031226 GO:0032870 GO:0042221 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

372

Amino Acids

40.42

Weight (kDa)

9.12

Isoelectric Point (pI)

31.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	12 - 148	5.5e-15	EamA-like transporter family
EamA	PF00892	185 - 323	2.6e-16	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012550)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09380
fragaria_vesca	FvH4_5g33020 FvH4_6g08060 FvH4_6g08060
malus_domestica	MD04G1189900.v1.1 MD04G1190500.v1.1 MD12G1202700.v1.1
prunus_persica	Prupe.6G308500_v2.0.a1
pyrus_communis	pycom04g16830 pycom12g18920
rosa_chinensis	RchiOBHm_Chr3g0457731
rosa_laevigata	RLG00000025190
rosa_roxburghii	Rroxscaffold_6G00421530
rosa_rugosa	Rorug03G0023000 Rorug03G0023100
rosa_samantha	Rh3AG083700 Rh3CG086400 Rh3DG087100
rosa_wichuraiana	Rw3G007170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	310, 507
AciI	CCGC	3 cut(s)	147, 294, 719
AclWI	GGATC	2 cut(s)	698, 734
AcoI	YGGCCR	2 cut(s)	33, 672
AcsI	RAATTY	1 cut(s)	489
AfiI	CCNNNNNNNGG	1 cut(s)	588
AflII	CTTAAG	1 cut(s)	574
AflIII	ACRYGT	1 cut(s)	840
AgsI	TTSAA	2 cut(s)	81, 95
AjuI	GAANNNNNNNTTGG	2 cut(s)	1049, 1081
AleI	CACNNNNGTG	1 cut(s)	661
Alw21I	GWGCWC	1 cut(s)	236
Alw26I	GTCTC	2 cut(s)	735, 980
AlwI	GGATC	2 cut(s)	698, 734
AoxI	GGCC	6 cut(s)	33, 246, 556, 672, 829, 1041
ApeKI	GCWGC	2 cut(s)	1072, 1075
ApoI	RAATTY	1 cut(s)	489
Asp700I	GAANNNNTTC	1 cut(s)	630
AspLEI	GCGC	2 cut(s)	311, 508
AspS9I	GGNCC	5 cut(s)	46, 106, 246, 557, 830
AsuC2I	CCSGG	1 cut(s)	952
AsuHPI	GGTGA	1 cut(s)	491
AvaII	GGWCC	2 cut(s)	46, 106
AxyI	CCTNAGG	1 cut(s)	583
BalI	TGGCCA	2 cut(s)	35, 674
BanII	GRGCYC	1 cut(s)	957
Bbv12I	GWGCWC	1 cut(s)	236
BbvI	GCAGC	2 cut(s)	1059, 1087
BccI	CCATC	3 cut(s)	169, 403, 664
BclI	TGATCA	1 cut(s)	709
BcnI	CCSGG	1 cut(s)	952
BcoDI	GTCTC	2 cut(s)	735, 980
BfaI	CTAG	3 cut(s)	476, 615, 791
BfmI	CTRYAG	3 cut(s)	10, 927, 1073
BfrI	CTTAAG	1 cut(s)	574
BisI	GCNGC	2 cut(s)	1073, 1076
BlsI	GCNGC	2 cut(s)	1074, 1077
Bme1390I	CCNGG	1 cut(s)	952
Bme18I	GGWCC	2 cut(s)	46, 106
BmgT120I	GGNCC	5 cut(s)	46, 106, 246, 557, 830
BmiI	GGNNCC	3 cut(s)	108, 247, 559
BmrFI	CCNGG	1 cut(s)	952
BmrI	ACTGGG	1 cut(s)	252
BmsI	GCATC	3 cut(s)	767, 1093, 1104
BmuI	ACTGGG	1 cut(s)	252
BpmI	CTGGAG	1 cut(s)	453
BpuEI	CTTGAG	1 cut(s)	365
BpuMI	CCSGG	1 cut(s)	952
BsaAI	YACGTR	1 cut(s)	841
BsaI	GGTCTC	1 cut(s)	735
BsaJI	CCNNGG	3 cut(s)	36, 457, 1044
Bsc4I	CCNNNNNNNGG	1 cut(s)	588
Bse1I	ACTGG	2 cut(s)	247, 500
Bse21I	CCTNAGG	1 cut(s)	583
Bse3DI	GCAATG	4 cut(s)	153, 311, 693, 879
BseDI	CCNNGG	3 cut(s)	36, 457, 1044
BseGI	GGATG	4 cut(s)	758, 1000, 1084, 1095
BseLI	CCNNNNNNNGG	1 cut(s)	588
BseMI	GCAATG	4 cut(s)	153, 311, 693, 879
BseNI	ACTGG	2 cut(s)	247, 500
BseRI	GAGGAG	1 cut(s)	854
BseXI	GCAGC	2 cut(s)	1059, 1087
BseYI	CCCAGC	1 cut(s)	888
BsgI	GTGCAG	1 cut(s)	771
Bsh1285I	CGRYCG	1 cut(s)	294
BshFI	GGCC	6 cut(s)	35, 248, 558, 674, 831, 1043
BsiEI	CGRYCG	1 cut(s)	294
BsiHKAI	GWGCWC	1 cut(s)	236
BsiSI	CCGG	1 cut(s)	951
BslFI	GGGAC	1 cut(s)	840
BslI	CCNNNNNNNGG	1 cut(s)	588
BsmAI	GTCTC	2 cut(s)	735, 980
BsmBI	CGTCTC	1 cut(s)	980
BsmFI	GGGAC	1 cut(s)	840
BsmI	GAATGC	2 cut(s)	593, 794
BsnI	GGCC	6 cut(s)	35, 248, 558, 674, 831, 1043
Bso31I	GGTCTC	1 cut(s)	735
Bsp1286I	GDGCHC	2 cut(s)	236, 957
Bsp143I	GATC	4 cut(s)	198, 690, 709, 739
Bsp19I	CCATGG	2 cut(s)	36, 457
BspACI	CCGC	3 cut(s)	147, 294, 719
BspANI	GGCC	6 cut(s)	35, 248, 558, 674, 831, 1043
BspLI	GGNNCC	3 cut(s)	108, 247, 559
BspMAI	CTGCAG	1 cut(s)	1077
BspPI	GGATC	2 cut(s)	698, 734
BspTI	CTTAAG	1 cut(s)	574
BspTNI	GGTCTC	1 cut(s)	735
BsrDI	GCAATG	4 cut(s)	153, 311, 693, 879
BsrI	ACTGG	2 cut(s)	247, 500
BssECI	CCNNGG	3 cut(s)	36, 457, 1044
BssMI	GATC	4 cut(s)	198, 690, 709, 739
BssT1I	CCWWGG	3 cut(s)	36, 457, 1044
Bst6I	CTCTTC	1 cut(s)	811
BstAFI	CTTAAG	1 cut(s)	574
BstAPI	GCANNNNNTGC	3 cut(s)	155, 302, 1081
BstBAI	YACGTR	1 cut(s)	841
BstC8I	GCNNGC	6 cut(s)	7, 55, 87, 307, 676, 886
BstDEI	CTNAG	2 cut(s)	402, 583
BstDSI	CCRYGG	2 cut(s)	36, 457
BstF5I	GGATG	4 cut(s)	758, 1000, 1084, 1095
BstHHI	GCGC	2 cut(s)	311, 508
BstKTI	GATC	4 cut(s)	201, 693, 712, 742
BstMAI	GTCTC	2 cut(s)	735, 980
BstMBI	GATC	4 cut(s)	198, 690, 709, 739
BstMCI	CGRYCG	1 cut(s)	294
BstMWI	GCNNNNNNNGC	7 cut(s)	59, 155, 164, 302, 704, 890, 1081
BstNSI	RCATGY	2 cut(s)	25, 309
BstSCI	CCNGG	1 cut(s)	950
BstSFI	CTRYAG	3 cut(s)	10, 927, 1073
BstV1I	GCAGC	2 cut(s)	1059, 1087
BstX2I	RGATCY	1 cut(s)	739
BstXI	CCANNNNNNTGG	1 cut(s)	43
BstYI	RGATCY	1 cut(s)	739
Bsu36I	CCTNAGG	1 cut(s)	583
BsuRI	GGCC	6 cut(s)	35, 248, 558, 674, 831, 1043
BtgI	CCRYGG	2 cut(s)	36, 457
BtgZI	GCGATG	1 cut(s)	422
BtsCI	GGATG	4 cut(s)	758, 1000, 1084, 1095
BtsI	GCAGTG	1 cut(s)	249
BtsIMutI	CAGTG	3 cut(s)	249, 493, 656
Cac8I	GCNNGC	6 cut(s)	7, 55, 87, 307, 676, 886
CfoI	GCGC	2 cut(s)	311, 508
Cfr13I	GGNCC	5 cut(s)	46, 106, 246, 557, 830
DdeI	CTNAG	2 cut(s)	402, 583
DpnI	GATC	4 cut(s)	200, 692, 711, 741
DpnII	GATC	4 cut(s)	198, 690, 709, 739
DraI	TTTAAA	1 cut(s)	282
EaeI	YGGCCR	2 cut(s)	33, 672
Eam1104I	CTCTTC	1 cut(s)	811
EarI	CTCTTC	1 cut(s)	811
Eco130I	CCWWGG	3 cut(s)	36, 457, 1044
Eco24I	GRGCYC	1 cut(s)	957
Eco31I	GGTCTC	1 cut(s)	735
Eco47I	GGWCC	2 cut(s)	46, 106
Eco81I	CCTNAGG	1 cut(s)	583
EcoO109I	RGGNCCY	1 cut(s)	830
EcoT14I	CCWWGG	3 cut(s)	36, 457, 1044
EcoT38I	GRGCYC	1 cut(s)	957
ErhI	CCWWGG	3 cut(s)	36, 457, 1044
Esp3I	CGTCTC	1 cut(s)	980
FalI	AAGNNNNNCTT	4 cut(s)	819, 851, 877, 909
FaqI	GGGAC	1 cut(s)	840
FauNDI	CATATG	1 cut(s)	502
FbaI	TGATCA	1 cut(s)	709
Fnu4HI	GCNGC	2 cut(s)	1073, 1076
FokI	GGATG	4 cut(s)	745, 1007, 1071, 1082
FriOI	GRGCYC	1 cut(s)	957
Fsp4HI	GCNGC	2 cut(s)	1073, 1076
FspAI	RTGCGCAY	1 cut(s)	310
FspBI	CTAG	3 cut(s)	476, 615, 791
FspI	TGCGCA	2 cut(s)	310, 507
GlaI	GCGC	2 cut(s)	310, 507
GluI	GCNGC	2 cut(s)	1073, 1076
GsaI	CCCAGC	1 cut(s)	892
GsuI	CTGGAG	1 cut(s)	453
HaeIII	GGCC	6 cut(s)	35, 248, 558, 674, 831, 1043
HapII	CCGG	1 cut(s)	951
HhaI	GCGC	2 cut(s)	311, 508
Hin6I	GCGC	2 cut(s)	309, 506
HinP1I	GCGC	2 cut(s)	309, 506
HindIII	AAGCTT	2 cut(s)	83, 628
HinfI	GANTC	3 cut(s)	95, 368, 482
HpaII	CCGG	1 cut(s)	951
HphI	GGTGA	1 cut(s)	491
Hpy188I	TCNGA	3 cut(s)	100, 362, 1089
Hpy188III	TCNNGA	3 cut(s)	220, 382, 432
HpyAV	CCTTC	1 cut(s)	985
HpyCH4IV	ACGT	4 cut(s)	203, 840, 915, 1104
HpyF10VI	GCNNNNNNNGC	7 cut(s)	59, 155, 164, 302, 704, 890, 1081
HpyF3I	CTNAG	2 cut(s)	402, 583
HpySE526I	ACGT	4 cut(s)	203, 840, 915, 1104
HspAI	GCGC	2 cut(s)	309, 506
Ksp22I	TGATCA	1 cut(s)	709
Kzo9I	GATC	4 cut(s)	198, 690, 709, 739
LmnI	GCTCC	6 cut(s)	163, 434, 551, 643, 867, 1069
Lsp1109I	GCAGC	2 cut(s)	1059, 1087
LweI	GCATC	3 cut(s)	767, 1093, 1104
MaeI	CTAG	3 cut(s)	476, 615, 791
MaeII	ACGT	4 cut(s)	203, 840, 915, 1104
MaeIII	GTNAC	3 cut(s)	441, 479, 1100
MalI	GATC	4 cut(s)	200, 692, 711, 741
MboI	GATC	4 cut(s)	198, 690, 709, 739
MboII	GAAGA	3 cut(s)	208, 790, 798
MfeI	CAATTG	1 cut(s)	417
MflI	RGATCY	1 cut(s)	739
MhlI	GDGCHC	2 cut(s)	236, 957
MlsI	TGGCCA	2 cut(s)	35, 674
MluCI	AATT	5 cut(s)	417, 489, 702, 774, 908
MluNI	TGGCCA	2 cut(s)	35, 674
MlyI	GAGTC	1 cut(s)	476
MmeI	TCCRAC	1 cut(s)	898
MnlI	CCTC	6 cut(s)	120, 773, 812, 843, 875, 1033
Mox20I	TGGCCA	2 cut(s)	35, 674
MroXI	GAANNNNTTC	1 cut(s)	630
MscI	TGGCCA	2 cut(s)	35, 674
MseI	TTAA	3 cut(s)	239, 281, 575
MslI	CAYNNNNRTG	3 cut(s)	35, 659, 661
Msp20I	TGGCCA	2 cut(s)	35, 674
MspCI	CTTAAG	1 cut(s)	574
MspI	CCGG	1 cut(s)	951
MspR9I	CCNGG	1 cut(s)	952
MunI	CAATTG	1 cut(s)	417
Mva1269I	GAATGC	2 cut(s)	593, 794
MwoI	GCNNNNNNNGC	7 cut(s)	59, 155, 164, 302, 704, 890, 1081
NciI	CCSGG	1 cut(s)	952
NcoI	CCATGG	2 cut(s)	36, 457
NdeI	CATATG	1 cut(s)	502
NdeII	GATC	4 cut(s)	198, 690, 709, 739
NlaIV	GGNNCC	3 cut(s)	108, 247, 559
NmuCI	GTSAC	2 cut(s)	479, 1100
NsbI	TGCGCA	2 cut(s)	310, 507
NspI	RCATGY	2 cut(s)	25, 309
OliI	CACNNNNGTG	1 cut(s)	661
PaeI	GCATGC	1 cut(s)	309
PcsI	WCGNNNNNNNCGW	1 cut(s)	296
PctI	GAATGC	2 cut(s)	593, 794
PdmI	GAANNNNTTC	1 cut(s)	630
PfeI	GAWTC	2 cut(s)	95, 368
PkrI	GCNGC	2 cut(s)	1074, 1077
PleI	GAGTC	1 cut(s)	476
PpsI	GAGTC	1 cut(s)	476
Ppu21I	YACGTR	1 cut(s)	841
PspFI	CCCAGC	1 cut(s)	888
PspN4I	GGNNCC	3 cut(s)	108, 247, 559
PspPI	GGNCC	5 cut(s)	46, 106, 246, 557, 830
PstI	CTGCAG	1 cut(s)	1077
PsuI	RGATCY	1 cut(s)	739
RseI	CAYNNNNRTG	3 cut(s)	35, 659, 661
SaqAI	TTAA	3 cut(s)	239, 281, 575
SatI	GCNGC	2 cut(s)	1073, 1076
Sau3AI	GATC	4 cut(s)	198, 690, 709, 739
Sau96I	GGNCC	5 cut(s)	46, 106, 246, 557, 830
SchI	GAGTC	1 cut(s)	476
ScrFI	CCNGG	1 cut(s)	952
SduI	GDGCHC	2 cut(s)	236, 957
SfaNI	GCATC	3 cut(s)	767, 1093, 1104
SfcI	CTRYAG	3 cut(s)	10, 927, 1073
SinI	GGWCC	2 cut(s)	46, 106
SmiMI	CAYNNNNRTG	3 cut(s)	35, 659, 661
SmlI	CTYRAG	2 cut(s)	380, 574
SmoI	CTYRAG	2 cut(s)	380, 574
SphI	GCATGC	1 cut(s)	309
Sse9I	AATT	5 cut(s)	417, 489, 702, 774, 908
SsiI	CCGC	3 cut(s)	147, 294, 719
SspMI	CTAG	3 cut(s)	476, 615, 791
StyD4I	CCNGG	1 cut(s)	950
StyI	CCWWGG	3 cut(s)	36, 457, 1044
TaiI	ACGT	4 cut(s)	206, 843, 918, 1107
TaqI	TCGA	2 cut(s)	290, 900
TaqII	GACCGA	1 cut(s)	208
TasI	AATT	5 cut(s)	417, 489, 702, 774, 908
TfiI	GAWTC	2 cut(s)	95, 368
Tru1I	TTAA	3 cut(s)	239, 281, 575
Tru9I	TTAA	3 cut(s)	239, 281, 575
TscAI	CASTG	3 cut(s)	256, 500, 663
TseFI	GTSAC	2 cut(s)	479, 1100
TseI	GCWGC	2 cut(s)	1072, 1075
Tsp45I	GTSAC	2 cut(s)	479, 1100
TspDTI	ATGAA	6 cut(s)	19, 83, 438, 592, 714, 798
TspGWI	ACGGA	1 cut(s)	1088
TspRI	CASTG	3 cut(s)	256, 500, 663
Vha464I	CTTAAG	1 cut(s)	574
VpaK11BI	GGWCC	2 cut(s)	46, 106
XapI	RAATTY	1 cut(s)	489
XceI	RCATGY	2 cut(s)	25, 309
XmnI	GAANNNNTTC	1 cut(s)	630
XspI	CTAG	3 cut(s)	476, 615, 791

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.