FvH4_5g35560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011711
Physical Location & Seq
Reverse (-)
26038334 .. 26039536
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35560.t1

Sequence Viewer

Length: 1203 bp
ATGGCGATCAATTTGGTCCAAGGTTTCCGAACCCAAATCCTCCCGACGCACAAAGCCAGCATTGGATTAATATACCCTGATGCCGAGTACTTAGTTATTAGGATCCCTGACTCACGAGTCTTGTTGTTTCTTGCTCGATCTGTCATCTTGGCTTTGGTGATCGTCTCCATTCCATGGTTGAACTCACTTGTTCCCATGACATCAACCGCTAATGAAATCATCGGCTCCTCCGCGACTAATCTCGAGCTCGTGCCACTCATCTTCCACGACTTGGCCAAAGAAGGCCTCATCAAAACCGGCCATGGCGAGAAGGCAGTGTTTGTTAACAATGGTGCCATACAAAGCTCAGAGATCCTCAAAGACAATGGAGTGGATCTCATTCACGAAGACCAACAGAACTCGGTCAACGACCAATCTGTTGACTTCGCCTTCGTCTCCGTCTTCCCTTCCGCCGTGAAGTTCATCGAAAGAACTCTCAAGGTTGGCGGCATTGTTGCGCTCCAGCTCAGCGAGGAAGCTTCCATGAAGTTCCGCATGCCTTCTAACTACAAGATGGTCTACCTCCGAAAATTCGACGCCACCGTCATGGCCATGAGGAAGATGAGTAGTTCATCGGTTTCAACTGCTACTAATTCTCCTCCCACGCGCCGACGTCTTCTATCCGAGGATGCAAAGAAGGAGGCATTGAAGAATCTCGAAGATGTGCTTCTCGAACCGCCACGTGCTAAATCGGGCAAATCGAGCCGATACTTGAAGAAAACTCGCTACCTTCCCGACTTGATGGGAGACTCGCTCGAGGGATACTCTCGCAGGATCTTCATCGACGTTGGCGGTAGCGGCACCAACTGGTTTGCCAAGAATTACCCGACTCGCAACAAGAATTTCGAGATGTACAAGATCGAGACAACCCTAACGGATGAGTCCGCCGGCAGCAAGGGGGTTCTTCAGACCAGTATGTCGGGGTGGTTGGAGAAGAATGTAAAGGAGGAAGAGTATGTTGTGATGAAGGCGGAGGCCGAAGTGGTGGAGGAAATGATGCAGGGAAAGGTGATGAACTTGGTTGATGAGCTTTTCTTGGAATGCAAGCCACAAGGGCATAATGGGAAAGGTAATGTGAGCACTCGTAGGGCTTACTGGCAGTGCTTGGCTTTGTACGGACAGCTGAGGGACGAAGGTGTTGCAGTGCACCAATGGTGGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

401

Amino Acids

44.73

Weight (kDa)

8.29

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 229 - 399 1.7e-77 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 116, 581, 955
AatII GACGTC 1 cut(s) 655
AccB1I GGYRCC 2 cut(s) 332, 839
AccB7I CCANNNNNTGG 2 cut(s) 174, 271
AccI GTMKAC 1 cut(s) 558
AccII CGCG 2 cut(s) 233, 646
AclWI GGATC 5 cut(s) 97, 110, 346, 381, 821
AcoI YGGCCR 3 cut(s) 273, 298, 588
AcsI RAATTY 2 cut(s) 569, 880
AcuI CTGAAG 1 cut(s) 929
AcvI CACGTG 1 cut(s) 722
AcyI GRCGYC 2 cut(s) 576, 652
AfaI GTAC 3 cut(s) 89, 893, 1154
AfiI CCNNNNNNNGG 3 cut(s) 174, 271, 1195
AgsI TTSAA 4 cut(s) 181, 621, 688, 754
AluBI AGCT 6 cut(s) 247, 345, 505, 518, 1069, 1162
AluI AGCT 6 cut(s) 247, 345, 505, 518, 1069, 1162
Alw21I GWGCWC 3 cut(s) 249, 1121, 1188
Alw26I GTCTC 4 cut(s) 169, 439, 780, 896
Alw44I GTGCAC 1 cut(s) 1184
AlwI GGATC 5 cut(s) 97, 110, 346, 381, 821
Ama87I CYCGRG 2 cut(s) 242, 794
AoxI GGCC 5 cut(s) 273, 283, 298, 588, 1014
ApaLI GTGCAC 1 cut(s) 1184
ApeKI GCWGC 1 cut(s) 930
ApoI RAATTY 2 cut(s) 569, 880
ArsI GACNNNNNNTTYG 2 cut(s) 413, 445
AseI ATTAAT 1 cut(s) 68
AspLEI GCGC 2 cut(s) 499, 648
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 2 cut(s) 169, 1060
AvaI CYCGRG 2 cut(s) 242, 794
AvaII GGWCC 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 1188
BalI TGGCCA 2 cut(s) 275, 590
BamHI GGATCC 1 cut(s) 102
BanI GGYRCC 2 cut(s) 332, 839
BanII GRGCYC 1 cut(s) 249
BauI CACGAG 2 cut(s) 114, 248
BbrPI CACGTG 1 cut(s) 722
BbsI GAAGAC 3 cut(s) 393, 433, 647
Bbv12I GWGCWC 3 cut(s) 249, 1121, 1188
BbvCI CCTCAGC 1 cut(s) 1163
BbvI GCAGC 1 cut(s) 942
BccI CCATC 2 cut(s) 547, 775
BceAI ACGGC 1 cut(s) 437
BcgI CGANNNNNNTGC 2 cut(s) 1160, 1194
BciVI GTATCC 1 cut(s) 794
BcoDI GTCTC 4 cut(s) 169, 439, 780, 896
BfuI GTATCC 1 cut(s) 794
BglI GCCNNNNNGGC 1 cut(s) 1093
BisI GCNGC 3 cut(s) 487, 838, 931
BlpI GCTNAGC 1 cut(s) 506
BlsI GCNGC 3 cut(s) 488, 839, 932
BmcAI AGTACT 1 cut(s) 89
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 2 cut(s) 242, 794
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 4 cut(s) 104, 226, 334, 841
BmsI GCATC 3 cut(s) 70, 658, 1026
BpiI GAAGAC 3 cut(s) 393, 433, 647
BplI GAGNNNNNCTC 6 cut(s) 360, 392, 777, 809, 788, 820
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 1163
Bpu1102I GCTNAGC 1 cut(s) 506
BpuEI CTTGAG 1 cut(s) 461
BsaAI YACGTR 1 cut(s) 722
BsaBI GATNNNNATC 1 cut(s) 818
BsaHI GRCGYC 2 cut(s) 576, 652
BsaJI CCNNGG 4 cut(s) 19, 173, 301, 663
BsaXI ACNNNNNCTCC 4 cut(s) 619, 649, 977, 1007
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 271, 1195
Bse118I RCCGGY 2 cut(s) 296, 926
Bse1I ACTGG 3 cut(s) 851, 951, 1139
Bse8I GATNNNNATC 1 cut(s) 818
BseDI CCNNGG 4 cut(s) 19, 173, 301, 663
BseGI GGATG 2 cut(s) 673, 922
BseJI GATNNNNATC 1 cut(s) 818
BseLI CCNNNNNNNGG 3 cut(s) 174, 271, 1195
BseMII CTCAG 3 cut(s) 360, 520, 1154
BseNI ACTGG 3 cut(s) 851, 951, 1139
BseRI GAGGAG 2 cut(s) 217, 627
BseSI GKGCMC 1 cut(s) 1188
BseXI GCAGC 1 cut(s) 942
Bsh1236I CGCG 2 cut(s) 233, 646
BshFI GGCC 5 cut(s) 275, 285, 300, 590, 1016
BshNI GGYRCC 2 cut(s) 332, 839
BsiHKAI GWGCWC 3 cut(s) 249, 1121, 1188
BsiHKCI CYCGRG 2 cut(s) 242, 794
BsiSI CCGG 2 cut(s) 297, 927
BslFI GGGAC 1 cut(s) 1181
BslI CCNNNNNNNGG 3 cut(s) 174, 271, 1195
BsmAI GTCTC 4 cut(s) 169, 439, 780, 896
BsmBI CGTCTC 2 cut(s) 169, 439
BsmFI GGGAC 1 cut(s) 1181
BsmI GAATGC 1 cut(s) 1085
BsnI GGCC 5 cut(s) 275, 285, 300, 590, 1016
BsoBI CYCGRG 2 cut(s) 242, 794
Bsp1286I GDGCHC 3 cut(s) 249, 1121, 1188
Bsp1407I TGTACA 1 cut(s) 891
Bsp143I GATC 8 cut(s) 6, 102, 137, 159, 351, 373, 813, 897
Bsp1720I GCTNAGC 1 cut(s) 506
Bsp19I CCATGG 2 cut(s) 173, 301
BspANI GGCC 5 cut(s) 275, 285, 300, 590, 1016
BspCNI CTCAG 3 cut(s) 359, 519, 1155
BspFNI CGCG 2 cut(s) 233, 646
BspLI GGNNCC 4 cut(s) 104, 226, 334, 841
BspPI GGATC 5 cut(s) 97, 110, 346, 381, 821
BspT107I GGYRCC 2 cut(s) 332, 839
BsrFI RCCGGY 2 cut(s) 296, 926
BsrGI TGTACA 1 cut(s) 891
BsrI ACTGG 3 cut(s) 851, 951, 1139
BssAI RCCGGY 2 cut(s) 296, 926
BssECI CCNNGG 4 cut(s) 19, 173, 301, 663
BssMI GATC 8 cut(s) 6, 102, 137, 159, 351, 373, 813, 897
BssNI GRCGYC 2 cut(s) 576, 652
BssSI CACGAG 2 cut(s) 114, 248
BssT1I CCWWGG 3 cut(s) 19, 173, 301
Bst2BI CACGAG 2 cut(s) 114, 248
Bst4CI ACNGT 1 cut(s) 583
Bst6I CTCTTC 1 cut(s) 984
BstACI GRCGYC 2 cut(s) 576, 652
BstAUI TGTACA 1 cut(s) 891
BstBAI YACGTR 1 cut(s) 722
BstC8I GCNNGC 4 cut(s) 58, 536, 928, 1085
BstDEI CTNAG 4 cut(s) 91, 346, 506, 1163
BstDSI CCRYGG 2 cut(s) 173, 301
BstF5I GGATG 2 cut(s) 673, 922
BstFNI CGCG 2 cut(s) 233, 646
BstHHI GCGC 2 cut(s) 499, 648
BstKTI GATC 8 cut(s) 9, 105, 140, 162, 354, 376, 816, 900
BstMAI GTCTC 4 cut(s) 169, 439, 780, 896
BstMBI GATC 8 cut(s) 6, 102, 137, 159, 351, 373, 813, 897
BstMWI GCNNNNNNNGC 3 cut(s) 741, 837, 1093
BstNSI RCATGY 1 cut(s) 538
BstSLI GKGCMC 1 cut(s) 1188
BstUI CGCG 2 cut(s) 233, 646
BstV1I GCAGC 1 cut(s) 942
BstV2I GAAGAC 3 cut(s) 393, 433, 647
BstX2I RGATCY 4 cut(s) 102, 351, 373, 813
BstXI CCANNNNNNTGG 1 cut(s) 586
BstYI RGATCY 4 cut(s) 102, 351, 373, 813
BsuI GTATCC 1 cut(s) 794
BsuRI GGCC 5 cut(s) 275, 285, 300, 590, 1016
BtgI CCRYGG 2 cut(s) 173, 301
BtsCI GGATG 2 cut(s) 673, 922
BtsI GCAGTG 3 cut(s) 321, 1145, 1188
BtsIMutI CAGTG 3 cut(s) 321, 1145, 1188
Cac8I GCNNGC 4 cut(s) 58, 536, 928, 1085
CfoI GCGC 2 cut(s) 499, 648
Cfr10I RCCGGY 2 cut(s) 296, 926
Cfr13I GGNCC 1 cut(s) 16
CseI GACGC 2 cut(s) 55, 584
Csp6I GTAC 3 cut(s) 88, 892, 1153
CviAII CATG 7 cut(s) 174, 196, 302, 523, 535, 586, 592
CviQI GTAC 3 cut(s) 88, 892, 1153
DdeI CTNAG 4 cut(s) 91, 346, 506, 1163
DpnI GATC 8 cut(s) 8, 104, 139, 161, 353, 375, 815, 899
DpnII GATC 8 cut(s) 6, 102, 137, 159, 351, 373, 813, 897
DrdI GACNNNNNNGTC 3 cut(s) 116, 581, 955
DseDI GACNNNNNNGTC 3 cut(s) 116, 581, 955
EaeI YGGCCR 3 cut(s) 273, 298, 588
Eam1104I CTCTTC 1 cut(s) 984
EarI CTCTTC 1 cut(s) 984
EciI GGCGGA 3 cut(s) 439, 913, 1025
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 3 cut(s) 19, 173, 301
Eco147I AGGCCT 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 929
Eco72I CACGTG 1 cut(s) 722
Eco88I CYCGRG 2 cut(s) 242, 794
EcoICRI GAGCTC 1 cut(s) 247
EcoT14I CCWWGG 3 cut(s) 19, 173, 301
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 3 cut(s) 19, 173, 301
Esp3I CGTCTC 2 cut(s) 169, 439
FaeI CATG 7 cut(s) 177, 199, 305, 526, 538, 589, 595
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FaqI GGGAC 1 cut(s) 1181
FatI CATG 7 cut(s) 173, 195, 301, 522, 534, 585, 591
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 3 cut(s) 487, 838, 931
FokI GGATG 2 cut(s) 680, 929
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 3 cut(s) 487, 838, 931
GlaI GCGC 2 cut(s) 498, 647
GluI GCNGC 3 cut(s) 487, 838, 931
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 5 cut(s) 275, 285, 300, 590, 1016
HapII CCGG 2 cut(s) 297, 927
HgaI GACGC 2 cut(s) 55, 584
HhaI GCGC 2 cut(s) 499, 648
Hin1I GRCGYC 2 cut(s) 576, 652
Hin1II CATG 7 cut(s) 177, 199, 305, 526, 538, 589, 595
Hin6I GCGC 2 cut(s) 497, 646
HinP1I GCGC 2 cut(s) 497, 646
HincII GTYRAC 3 cut(s) 325, 406, 421
HindII GTYRAC 3 cut(s) 325, 406, 421
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 6 cut(s) 110, 117, 691, 788, 868, 920
HpaI GTTAAC 1 cut(s) 325
HpaII CCGG 2 cut(s) 297, 927
HphI GGTGA 2 cut(s) 169, 1060
Hpy166II GTNNAC 5 cut(s) 325, 406, 421, 559, 1186
Hpy188I TCNGA 5 cut(s) 29, 349, 566, 664, 948
Hpy188III TCNNGA 9 cut(s) 43, 114, 242, 383, 695, 710, 773, 886, 901
Hpy8I GTNNAC 5 cut(s) 325, 406, 421, 559, 1186
Hpy99I CGWCG 4 cut(s) 49, 578, 654, 827
HpyAV CCTTC 9 cut(s) 275, 304, 439, 456, 549, 670, 779, 1000, 1166
HpyCH4III ACNGT 1 cut(s) 583
HpyCH4IV ACGT 3 cut(s) 652, 721, 825
HpyCH4V TGCA 5 cut(s) 671, 1039, 1083, 1181, 1186
HpyF10VI GCNNNNNNNGC 3 cut(s) 741, 837, 1093
HpyF3I CTNAG 4 cut(s) 91, 346, 506, 1163
HpySE526I ACGT 3 cut(s) 652, 721, 825
Hsp92I GRCGYC 2 cut(s) 576, 652
Hsp92II CATG 7 cut(s) 177, 199, 305, 526, 538, 589, 595
HspAI GCGC 2 cut(s) 497, 646
KroI GCCGGC 1 cut(s) 926
KroNI GCCGGC 1 cut(s) 928
KspAI GTTAAC 1 cut(s) 325
Kzo9I GATC 8 cut(s) 6, 102, 137, 159, 351, 373, 813, 897
LmnI GCTCC 2 cut(s) 230, 504
Lsp1109I GCAGC 1 cut(s) 942
LweI GCATC 3 cut(s) 70, 658, 1026
MaeII ACGT 3 cut(s) 652, 721, 825
MalI GATC 8 cut(s) 8, 104, 139, 161, 353, 375, 815, 899
MboI GATC 8 cut(s) 6, 102, 137, 159, 351, 373, 813, 897
MflI RGATCY 4 cut(s) 102, 351, 373, 813
MhlI GDGCHC 3 cut(s) 249, 1121, 1188
MlsI TGGCCA 2 cut(s) 275, 590
MluCI AATT 5 cut(s) 10, 569, 631, 859, 880
MluNI TGGCCA 2 cut(s) 275, 590
MlyI GAGTC 5 cut(s) 104, 126, 782, 862, 929
MmeI TCCRAC 1 cut(s) 948
Mox20I TGGCCA 2 cut(s) 275, 590
MroNI GCCGGC 1 cut(s) 926
MscI TGGCCA 2 cut(s) 275, 590
MseI TTAA 3 cut(s) 68, 324, 1201
MslI CAYNNNNRTG 2 cut(s) 584, 590
Msp20I TGGCCA 2 cut(s) 275, 590
MspA1I CMGCKG 1 cut(s) 1162
MspI CCGG 2 cut(s) 297, 927
Mva1269I GAATGC 1 cut(s) 1085
MvnI CGCG 2 cut(s) 233, 646
MwoI GCNNNNNNNGC 3 cut(s) 741, 837, 1093
NaeI GCCGGC 1 cut(s) 928
NcoI CCATGG 2 cut(s) 173, 301
NdeII GATC 8 cut(s) 6, 102, 137, 159, 351, 373, 813, 897
NgoMIV GCCGGC 1 cut(s) 926
NlaIII CATG 7 cut(s) 177, 199, 305, 526, 538, 589, 595
NlaIV GGNNCC 4 cut(s) 104, 226, 334, 841
NmeAIII GCCGAG 1 cut(s) 109
NspI RCATGY 1 cut(s) 538
PaeI GCATGC 1 cut(s) 538
PaeR7I CTCGAG 2 cut(s) 242, 794
PceI AGGCCT 1 cut(s) 285
PcsI WCGNNNNNNNCGW 2 cut(s) 579, 737
PctI GAATGC 1 cut(s) 1085
PdiI GCCGGC 1 cut(s) 928
PfeI GAWTC 1 cut(s) 691
PflMI CCANNNNNTGG 2 cut(s) 174, 271
PkrI GCNGC 3 cut(s) 488, 839, 932
PleI GAGTC 5 cut(s) 104, 125, 782, 862, 928
PmaCI CACGTG 1 cut(s) 722
PmlI CACGTG 1 cut(s) 722
PpsI GAGTC 5 cut(s) 104, 125, 782, 862, 928
Ppu21I YACGTR 1 cut(s) 722
PshBI ATTAAT 1 cut(s) 68
Psp124BI GAGCTC 1 cut(s) 249
PspCI CACGTG 1 cut(s) 722
PspN4I GGNNCC 4 cut(s) 104, 226, 334, 841
PspPI GGNCC 1 cut(s) 16
PspXI VCTCGAGB 1 cut(s) 794
PsuI RGATCY 4 cut(s) 102, 351, 373, 813
PvuII CAGCTG 1 cut(s) 1162
RsaI GTAC 3 cut(s) 89, 893, 1154
RsaNI GTAC 3 cut(s) 88, 892, 1153
RseI CAYNNNNRTG 2 cut(s) 584, 590
SacI GAGCTC 1 cut(s) 249
SaqAI TTAA 3 cut(s) 68, 324, 1201
SatI GCNGC 3 cut(s) 487, 838, 931
Sau3AI GATC 8 cut(s) 6, 102, 137, 159, 351, 373, 813, 897
Sau96I GGNCC 1 cut(s) 16
ScaI AGTACT 1 cut(s) 89
SchI GAGTC 5 cut(s) 104, 126, 782, 862, 929
SduI GDGCHC 3 cut(s) 249, 1121, 1188
SfaNI GCATC 3 cut(s) 70, 658, 1026
Sfr274I CTCGAG 2 cut(s) 242, 794
SinI GGWCC 1 cut(s) 16
SlaI CTCGAG 2 cut(s) 242, 794
SmiMI CAYNNNNRTG 2 cut(s) 584, 590
SmlI CTYRAG 3 cut(s) 242, 476, 794
SmoI CTYRAG 3 cut(s) 242, 476, 794
SphI GCATGC 1 cut(s) 538
Sse9I AATT 5 cut(s) 10, 569, 631, 859, 880
SseBI AGGCCT 1 cut(s) 285
SstI GAGCTC 1 cut(s) 249
StuI AGGCCT 1 cut(s) 285
StyI CCWWGG 3 cut(s) 19, 173, 301
TaaI ACNGT 1 cut(s) 583
TaiI ACGT 3 cut(s) 655, 724, 828
TaqII GACCGA 1 cut(s) 391
TasI AATT 5 cut(s) 10, 569, 631, 859, 880
TatI WGTACW 2 cut(s) 87, 891
TauI GCSGC 2 cut(s) 489, 840
TfiI GAWTC 1 cut(s) 691
Tru1I TTAA 3 cut(s) 68, 324, 1201
Tru9I TTAA 3 cut(s) 68, 324, 1201
TscAI CASTG 3 cut(s) 321, 1145, 1188
TseI GCWGC 1 cut(s) 930
TspDTI ATGAA 7 cut(s) 228, 451, 539, 600, 808, 1019, 1067
TspGWI ACGGA 3 cut(s) 427, 929, 1170
TspRI CASTG 3 cut(s) 321, 1145, 1188
Van91I CCANNNNNTGG 2 cut(s) 174, 271
VneI GTGCAC 1 cut(s) 1184
VpaK11BI GGWCC 1 cut(s) 16
VspI ATTAAT 1 cut(s) 68
XapI RAATTY 2 cut(s) 569, 880
XceI RCATGY 1 cut(s) 538
XhoI CTCGAG 2 cut(s) 242, 794
XmiI GTMKAC 1 cut(s) 558
ZraI GACGTC 1 cut(s) 653
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.