RLG00000001046

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011711
Physical Location & Seq
Forward (+)
9755147 .. 9756663
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001046

Sequence Viewer

Length: 1266 bp
ATGGAGGTTCAGAGGATTAGGCAGTTGATGTTATTATGGTCTAGAATGCAAAGCTCCCGTGTGGCCCTCGGCTTCCGAACCAAACAACACCTGGCACACAAAGCCACCATCGGATTCTACCCCGATGTCAAGTACTTAGTTTTTAGGATCCCTGACTCCCGAGTCTTGTTGTTTCTGGCTCGATGTTCCATCATGGCTTTCGTGATCGTCTCCGTTCCGTGGTTGAAGTCACTTGTTCCCACAACAGCCACCGCTAATGGAATCATCAGCTTCCCCGCGGCCCCTAATATCGAGCTTGTGCCACTCATCTTCCGCGACTTGGCCAACGAAGGCCTCATCAAAACCCGTCATGGCGAGAAGGCAGTGTTTGTCAGCAGTGGCAATGCTGAAGAAGCCATTCAAAGCTCACAGATCCTCGAAGACAACGAAATGGATCTCATTTCCGTGAACGACGAAAACCAACAGAACTCGATCAACGACCAATCTGTTGACTTCGCCTTCGTTTCCGTCGTCCCTTCCGCCATGAAGTTCATCGAAAGGACTCTCAAGGTTGGCGGCATTGTTGCGCTCCAGGACCTCTCCGCCAGGTTTCACATGCCTTCTAACTACAAGATGGTCTACCTCCGAAAACTCGACGCCACCGTGGTGGCCATGAGGAAGATGAGTTCATCGATTTCAACTACTACTTCTTTCACTCGCCGACGCCTATTTGCTGAGGAGGCAAAGAAGGAAGCATTGAAGAATCTCGAAGATGTGCTGCTCGAACCCCCACGTGCTTCATCTGGCAAGTCGAGCCGATACTTGAAGAAAACTCGCTACCTACCCGACTTGATGGGAGACTCGCTCGAGGGATACCCTCGCAGGGTCTTCATCGACGTTGGCTTGCCTGAAAAGGAAGGCGGTAGTGGCACCAGCTGGTTCGCCAAGAACTACCCGACTCGCAACAAGAATTTTGAGATGTACAAGATTGAGACAACCCTGATCGACGAGTCCGGCAAGGGGGTTCTTCAGACCGGTATGTCGGGGTGGTTGGAGAAGAATGTGAAGGAGGAAGAGTATGTTGTGATGAAGGCCGAGGCCGAAGTGGTGGAGGAAATGATGCAGGGTAAGGTGATGAACTTGGTCGATGAGCTTTTCTTGGAATGCAAGCCACAAGGGCACAATGGGAAAGGTAATTTGAGCACTCGTAGGGCTTACTGGCAGTGCTTGGCTTTGTACGGACAGCTCAGGGATGAAGGTGTTGCAGTGCACCAATGGTGGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

422

Amino Acids

47.6

Weight (kDa)

8.87

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 246 - 420 1.5e-81 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 161, 1018
AccB1I GGYRCC 1 cut(s) 908
AccI GTMKAC 1 cut(s) 618
AccII CGCG 2 cut(s) 278, 315
AciI CCGC 8 cut(s) 252, 276, 278, 313, 519, 555, 582, 900
AclWI GGATC 4 cut(s) 142, 155, 406, 441
AcoI YGGCCR 2 cut(s) 321, 648
AcsI RAATTY 1 cut(s) 949
AcuI CTGAAG 2 cut(s) 408, 992
AcvI CACGTG 1 cut(s) 773
AcyI GRCGYC 2 cut(s) 636, 703
AfaI GTAC 3 cut(s) 134, 962, 1217
AfiI CCNNNNNNNGG 5 cut(s) 219, 319, 862, 999, 1258
AgeI ACCGGT 1 cut(s) 1013
AgsI TTSAA 5 cut(s) 226, 401, 678, 739, 805
AjnI CCWGG 3 cut(s) 90, 570, 584
AleI CACNNNNGTG 1 cut(s) 644
AloI GAACNNNNNNTCC 2 cut(s) 649, 681
AluBI AGCT 7 cut(s) 54, 270, 295, 405, 915, 1132, 1225
AluI AGCT 7 cut(s) 54, 270, 295, 405, 915, 1132, 1225
Alw21I GWGCWC 2 cut(s) 1184, 1251
Alw26I GTCTC 3 cut(s) 214, 831, 965
Alw44I GTGCAC 1 cut(s) 1247
AlwI GGATC 4 cut(s) 142, 155, 406, 441
Ama87I CYCGRG 2 cut(s) 159, 845
AoxI GGCC 7 cut(s) 63, 279, 321, 331, 648, 1071, 1077
ApaLI GTGCAC 1 cut(s) 1247
ApeKI GCWGC 1 cut(s) 757
ApoI RAATTY 1 cut(s) 949
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
AsiGI ACCGGT 1 cut(s) 1013
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 1 cut(s) 568
AspS9I GGNCC 3 cut(s) 64, 280, 574
AsuHPI GGTGA 1 cut(s) 1123
AvaI CYCGRG 2 cut(s) 159, 845
AvaII GGWCC 1 cut(s) 574
BaeGI GKGCMC 2 cut(s) 1161, 1251
BalI TGGCCA 2 cut(s) 323, 650
BamHI GGATCC 1 cut(s) 147
BanI GGYRCC 1 cut(s) 908
BbrPI CACGTG 1 cut(s) 773
BbsI GAAGAC 2 cut(s) 426, 859
Bbv12I GWGCWC 2 cut(s) 1184, 1251
BbvCI CCTCAGC 1 cut(s) 714
BbvI GCAGC 1 cut(s) 744
BccI CCATC 4 cut(s) 116, 197, 607, 826
BciT130I CCWGG 3 cut(s) 92, 572, 586
BciVI GTATCC 1 cut(s) 845
BcoDI GTCTC 3 cut(s) 214, 831, 965
BfaI CTAG 1 cut(s) 42
BfuI GTATCC 1 cut(s) 845
BglI GCCNNNNNGGC 1 cut(s) 1156
BisI GCNGC 3 cut(s) 279, 556, 758
BlsI GCNGC 3 cut(s) 280, 557, 759
BmcAI AGTACT 1 cut(s) 134
Bme1390I CCNGG 3 cut(s) 92, 572, 586
Bme18I GGWCC 1 cut(s) 574
BmeT110I CYCGRG 2 cut(s) 159, 845
BmgT120I GGNCC 3 cut(s) 64, 280, 574
BmiI GGNNCC 3 cut(s) 149, 282, 910
BmrFI CCNGG 3 cut(s) 92, 572, 586
BmsI GCATC 1 cut(s) 1089
BpiI GAAGAC 2 cut(s) 426, 859
BplI GAGNNNNNCTC 2 cut(s) 828, 860
BpmI CTGGAG 1 cut(s) 554
Bpu10I CCTNAGC 2 cut(s) 714, 1226
BpuEI CTTGAG 1 cut(s) 530
Bsa29I ATCGAT 1 cut(s) 671
BsaAI YACGTR 1 cut(s) 773
BsaHI GRCGYC 2 cut(s) 636, 703
BsaJI CCNNGG 5 cut(s) 67, 218, 276, 642, 1074
BsaWI WCCGGW 1 cut(s) 1013
BsaXI ACNNNNNCTCC 2 cut(s) 1040, 1070
Bsc4I CCNNNNNNNGG 5 cut(s) 219, 319, 862, 999, 1258
Bse118I RCCGGY 1 cut(s) 1013
Bse1I ACTGG 1 cut(s) 1202
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 3 cut(s) 92, 572, 586
BseCI ATCGAT 1 cut(s) 671
BseDI CCNNGG 5 cut(s) 67, 218, 276, 642, 1074
BseGI GGATG 1 cut(s) 1237
BseLI CCNNNNNNNGG 5 cut(s) 219, 319, 862, 999, 1258
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 2 cut(s) 705, 1240
BseNI ACTGG 1 cut(s) 1202
BseRI GAGGAG 1 cut(s) 731
BseSI GKGCMC 2 cut(s) 1161, 1251
BseXI GCAGC 1 cut(s) 744
Bsh1236I CGCG 2 cut(s) 278, 315
BshFI GGCC 7 cut(s) 65, 281, 323, 333, 650, 1073, 1079
BshNI GGYRCC 1 cut(s) 908
BshTI ACCGGT 1 cut(s) 1013
BshVI ATCGAT 1 cut(s) 671
BsiHKAI GWGCWC 2 cut(s) 1184, 1251
BsiHKCI CYCGRG 2 cut(s) 159, 845
BsiSI CCGG 2 cut(s) 993, 1014
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 5 cut(s) 219, 319, 862, 999, 1258
BsmAI GTCTC 3 cut(s) 214, 831, 965
BsmBI CGTCTC 1 cut(s) 214
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 2 cut(s) 51, 1148
BsnI GGCC 7 cut(s) 65, 281, 323, 333, 650, 1073, 1079
BsoBI CYCGRG 2 cut(s) 159, 845
Bsp1286I GDGCHC 3 cut(s) 1161, 1184, 1251
Bsp1407I TGTACA 1 cut(s) 960
Bsp143I GATC 6 cut(s) 147, 204, 411, 433, 471, 981
BspACI CCGC 8 cut(s) 252, 276, 278, 313, 519, 555, 582, 900
BspANI GGCC 7 cut(s) 65, 281, 323, 333, 650, 1073, 1079
BspCNI CTCAG 2 cut(s) 706, 1239
BspDI ATCGAT 1 cut(s) 671
BspFNI CGCG 2 cut(s) 278, 315
BspLI GGNNCC 3 cut(s) 149, 282, 910
BspPI GGATC 4 cut(s) 142, 155, 406, 441
BspT107I GGYRCC 1 cut(s) 908
BsrDI GCAATG 1 cut(s) 388
BsrFI RCCGGY 1 cut(s) 1013
BsrGI TGTACA 1 cut(s) 960
BsrI ACTGG 1 cut(s) 1202
BssAI RCCGGY 1 cut(s) 1013
BssECI CCNNGG 5 cut(s) 67, 218, 276, 642, 1074
BssMI GATC 6 cut(s) 147, 204, 411, 433, 471, 981
BssNI GRCGYC 2 cut(s) 636, 703
Bst2UI CCWGG 3 cut(s) 92, 572, 586
Bst4CI ACNGT 1 cut(s) 643
Bst6I CTCTTC 1 cut(s) 1047
BstACI GRCGYC 2 cut(s) 636, 703
BstAUI TGTACA 1 cut(s) 960
BstBAI YACGTR 1 cut(s) 773
BstC8I GCNNGC 2 cut(s) 884, 1148
BstDEI CTNAG 3 cut(s) 136, 714, 1226
BstDSI CCRYGG 3 cut(s) 218, 276, 642
BstF5I GGATG 1 cut(s) 1237
BstFNI CGCG 2 cut(s) 278, 315
BstHHI GCGC 1 cut(s) 568
BstKTI GATC 6 cut(s) 150, 207, 414, 436, 474, 984
BstMAI GTCTC 3 cut(s) 214, 831, 965
BstMBI GATC 6 cut(s) 147, 204, 411, 433, 471, 981
BstMWI GCNNNNNNNGC 6 cut(s) 101, 392, 719, 792, 906, 1156
BstNI CCWGG 3 cut(s) 92, 572, 586
BstNSI RCATGY 1 cut(s) 598
BstSCI CCNGG 3 cut(s) 90, 570, 584
BstSLI GKGCMC 2 cut(s) 1161, 1251
BstUI CGCG 2 cut(s) 278, 315
BstV1I GCAGC 1 cut(s) 744
BstV2I GAAGAC 2 cut(s) 426, 859
BstX2I RGATCY 3 cut(s) 147, 411, 433
BstXI CCANNNNNNTGG 1 cut(s) 646
BstYI RGATCY 3 cut(s) 147, 411, 433
Bsu15I ATCGAT 1 cut(s) 671
BsuI GTATCC 1 cut(s) 845
BsuRI GGCC 7 cut(s) 65, 281, 323, 333, 650, 1073, 1079
BsuTUI ATCGAT 1 cut(s) 671
BtgI CCRYGG 3 cut(s) 218, 276, 642
BtsCI GGATG 1 cut(s) 1237
BtsI GCAGTG 4 cut(s) 369, 382, 1208, 1251
BtsIMutI CAGTG 4 cut(s) 369, 382, 1208, 1251
Cac8I GCNNGC 2 cut(s) 884, 1148
CfoI GCGC 1 cut(s) 568
Cfr10I RCCGGY 1 cut(s) 1013
Cfr13I GGNCC 3 cut(s) 64, 280, 574
Cfr42I CCGCGG 1 cut(s) 279
ClaI ATCGAT 1 cut(s) 671
CseI GACGC 2 cut(s) 644, 711
Csp6I GTAC 3 cut(s) 133, 961, 1216
CspAI ACCGGT 1 cut(s) 1013
CviAII CATG 5 cut(s) 193, 350, 523, 595, 652
CviQI GTAC 3 cut(s) 133, 961, 1216
DdeI CTNAG 3 cut(s) 136, 714, 1226
DpnI GATC 6 cut(s) 149, 206, 413, 435, 473, 983
DpnII GATC 6 cut(s) 147, 204, 411, 433, 471, 981
DrdI GACNNNNNNGTC 2 cut(s) 161, 1018
DseDI GACNNNNNNGTC 2 cut(s) 161, 1018
EaeI YGGCCR 2 cut(s) 321, 648
Eam1104I CTCTTC 1 cut(s) 1047
EarI CTCTTC 1 cut(s) 1047
EciI GGCGGA 2 cut(s) 508, 571
Eco147I AGGCCT 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 574
Eco57I CTGAAG 2 cut(s) 408, 992
Eco72I CACGTG 1 cut(s) 773
Eco88I CYCGRG 2 cut(s) 159, 845
EcoO109I RGGNCCY 1 cut(s) 574
EcoRII CCWGG 3 cut(s) 90, 570, 584
Esp3I CGTCTC 1 cut(s) 214
FaeI CATG 5 cut(s) 196, 353, 526, 598, 655
FaiI YATR 8 cut(s) 37, 194, 351, 524, 596, 653, 1019, 1059
FaqI GGGAC 1 cut(s) 497
FatI CATG 5 cut(s) 192, 349, 522, 594, 651
FauI CCCGC 1 cut(s) 283
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 3 cut(s) 279, 556, 758
FokI GGATG 1 cut(s) 1244
Fsp4HI GCNGC 3 cut(s) 279, 556, 758
FspBI CTAG 1 cut(s) 42
GlaI GCGC 1 cut(s) 567
GluI GCNGC 3 cut(s) 279, 556, 758
GsuI CTGGAG 1 cut(s) 554
HaeIII GGCC 7 cut(s) 65, 281, 323, 333, 650, 1073, 1079
HapII CCGG 2 cut(s) 993, 1014
HgaI GACGC 2 cut(s) 644, 711
HhaI GCGC 1 cut(s) 568
Hin1I GRCGYC 2 cut(s) 636, 703
Hin1II CATG 5 cut(s) 196, 353, 526, 598, 655
Hin6I GCGC 1 cut(s) 566
HinP1I GCGC 1 cut(s) 566
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 9 cut(s) 114, 155, 162, 261, 541, 742, 839, 937, 989
HpaII CCGG 2 cut(s) 993, 1014
HphI GGTGA 1 cut(s) 1123
Hpy166II GTNNAC 4 cut(s) 448, 490, 619, 1249
Hpy188I TCNGA 5 cut(s) 12, 77, 113, 626, 1011
Hpy188III TCNNGA 4 cut(s) 42, 159, 202, 746
Hpy8I GTNNAC 4 cut(s) 448, 490, 619, 1249
Hpy99I CGWCG 6 cut(s) 455, 512, 638, 705, 878, 989
HpyCH4III ACNGT 1 cut(s) 643
HpyCH4IV ACGT 2 cut(s) 772, 876
HpyCH4V TGCA 5 cut(s) 49, 1102, 1146, 1244, 1249
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 392, 719, 792, 906, 1156
HpyF3I CTNAG 3 cut(s) 136, 714, 1226
HpySE526I ACGT 2 cut(s) 772, 876
Hsp92I GRCGYC 2 cut(s) 636, 703
Hsp92II CATG 5 cut(s) 196, 353, 526, 598, 655
HspAI GCGC 1 cut(s) 566
KspI CCGCGG 1 cut(s) 279
Kzo9I GATC 6 cut(s) 147, 204, 411, 433, 471, 981
LmnI GCTCC 2 cut(s) 59, 573
Lsp1109I GCAGC 1 cut(s) 744
LweI GCATC 1 cut(s) 1089
MaeI CTAG 1 cut(s) 42
MaeII ACGT 2 cut(s) 772, 876
MaeIII GTNAC 1 cut(s) 228
MalI GATC 6 cut(s) 149, 206, 413, 435, 473, 983
MboI GATC 6 cut(s) 147, 204, 411, 433, 471, 981
MflI RGATCY 3 cut(s) 147, 411, 433
MhlI GDGCHC 3 cut(s) 1161, 1184, 1251
MlsI TGGCCA 2 cut(s) 323, 650
MluCI AATT 2 cut(s) 949, 1174
MluNI TGGCCA 2 cut(s) 323, 650
MlyI GAGTC 6 cut(s) 149, 171, 535, 833, 931, 998
MmeI TCCRAC 1 cut(s) 1011
Mox20I TGGCCA 2 cut(s) 323, 650
MroXI GAANNNNTTC 1 cut(s) 396
MscI TGGCCA 2 cut(s) 323, 650
MseI TTAA 1 cut(s) 1264
MslI CAYNNNNRTG 2 cut(s) 443, 644
Msp20I TGGCCA 2 cut(s) 323, 650
MspA1I CMGCKG 2 cut(s) 278, 915
MspI CCGG 2 cut(s) 993, 1014
MspR9I CCNGG 3 cut(s) 92, 572, 586
Mva1269I GAATGC 2 cut(s) 51, 1148
MvaI CCWGG 3 cut(s) 92, 572, 586
MvnI CGCG 2 cut(s) 278, 315
MwoI GCNNNNNNNGC 6 cut(s) 101, 392, 719, 792, 906, 1156
NdeII GATC 6 cut(s) 147, 204, 411, 433, 471, 981
NlaIII CATG 5 cut(s) 196, 353, 526, 598, 655
NlaIV GGNNCC 3 cut(s) 149, 282, 910
NmeAIII GCCGAG 2 cut(s) 48, 1099
NmuCI GTSAC 1 cut(s) 228
NspI RCATGY 1 cut(s) 598
OliI CACNNNNGTG 1 cut(s) 644
PaeR7I CTCGAG 1 cut(s) 845
PceI AGGCCT 1 cut(s) 333
PcsI WCGNNNNNNNCGW 3 cut(s) 423, 507, 639
PctI GAATGC 2 cut(s) 51, 1148
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 3 cut(s) 114, 261, 742
PfoI TCCNGGA 1 cut(s) 570
PinAI ACCGGT 1 cut(s) 1013
PkrI GCNGC 3 cut(s) 280, 557, 759
PleI GAGTC 6 cut(s) 149, 170, 535, 833, 931, 997
PmaCI CACGTG 1 cut(s) 773
PmlI CACGTG 1 cut(s) 773
PpsI GAGTC 6 cut(s) 149, 170, 535, 833, 931, 997
Ppu21I YACGTR 1 cut(s) 773
PpuMI RGGWCCY 1 cut(s) 574
Psp5II RGGWCCY 1 cut(s) 574
Psp6I CCWGG 3 cut(s) 90, 570, 584
PspCI CACGTG 1 cut(s) 773
PspGI CCWGG 3 cut(s) 90, 570, 584
PspN4I GGNNCC 3 cut(s) 149, 282, 910
PspPI GGNCC 3 cut(s) 64, 280, 574
PspPPI RGGWCCY 1 cut(s) 574
PspXI VCTCGAGB 1 cut(s) 845
PsuI RGATCY 3 cut(s) 147, 411, 433
PvuII CAGCTG 1 cut(s) 915
RsaI GTAC 3 cut(s) 134, 962, 1217
RsaNI GTAC 3 cut(s) 133, 961, 1216
RseI CAYNNNNRTG 2 cut(s) 443, 644
SacII CCGCGG 1 cut(s) 279
SaqAI TTAA 1 cut(s) 1264
SatI GCNGC 3 cut(s) 279, 556, 758
Sau3AI GATC 6 cut(s) 147, 204, 411, 433, 471, 981
Sau96I GGNCC 3 cut(s) 64, 280, 574
ScaI AGTACT 1 cut(s) 134
SchI GAGTC 6 cut(s) 149, 171, 535, 833, 931, 998
ScrFI CCNGG 3 cut(s) 92, 572, 586
SduI GDGCHC 3 cut(s) 1161, 1184, 1251
SfaNI GCATC 1 cut(s) 1089
Sfr274I CTCGAG 1 cut(s) 845
Sfr303I CCGCGG 1 cut(s) 279
SgrBI CCGCGG 1 cut(s) 279
SinI GGWCC 1 cut(s) 574
SlaI CTCGAG 1 cut(s) 845
SmiMI CAYNNNNRTG 2 cut(s) 443, 644
SmlI CTYRAG 2 cut(s) 545, 845
SmoI CTYRAG 2 cut(s) 545, 845
Sse9I AATT 2 cut(s) 949, 1174
SseBI AGGCCT 1 cut(s) 333
SsiI CCGC 8 cut(s) 252, 276, 278, 313, 519, 555, 582, 900
SspMI CTAG 1 cut(s) 42
StuI AGGCCT 1 cut(s) 333
StyD4I CCNGG 3 cut(s) 90, 570, 584
TaaI ACNGT 1 cut(s) 643
TaiI ACGT 2 cut(s) 775, 879
TasI AATT 2 cut(s) 949, 1174
TatI WGTACW 2 cut(s) 132, 960
TauI GCSGC 2 cut(s) 281, 558
TfiI GAWTC 3 cut(s) 114, 261, 742
Tru1I TTAA 1 cut(s) 1264
Tru9I TTAA 1 cut(s) 1264
TscAI CASTG 4 cut(s) 369, 382, 1208, 1251
TseFI GTSAC 1 cut(s) 228
TseI GCWGC 1 cut(s) 757
Tsp45I GTSAC 1 cut(s) 228
TspDTI ATGAA 8 cut(s) 520, 539, 657, 768, 859, 1082, 1130, 1248
TspGWI ACGGA 5 cut(s) 202, 207, 433, 496, 1233
TspRI CASTG 4 cut(s) 369, 382, 1208, 1251
VneI GTGCAC 1 cut(s) 1247
VpaK11BI GGWCC 1 cut(s) 574
XapI RAATTY 1 cut(s) 949
XbaI TCTAGA 1 cut(s) 41
XceI RCATGY 1 cut(s) 598
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XhoI CTCGAG 1 cut(s) 845
XmiI GTMKAC 1 cut(s) 618
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 1 cut(s) 42
ZrmI AGTACT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.