MD08G1195700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011711
Physical Location & Seq
Forward (+)
25228516 .. 25229754
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1195700.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGCGATCAATTTGATCCAAGGTTTCCGTACCAAATGCCACCCAAAGCATGCTGCTCCAATCGGATTGTATCCAGATGCCAAGTACTTGGTTATTCGGATTCCCAATTCCAGAGCCTTGATCCTCGTTGCTCGTTCTTTGATCTTGGCTTTGTGCATCGCCTCACTCCCGTGGTTGAACTCACTCCTTCCCACAGCAACCACTTCTCTGCCTGAAATAATGAGCTCATCCGACGTGGTTAATCTGGAGCTTCTGCCTTTGATCTTCCGCGACTTGGCCAACGAAGGTCTATACAGGAAAGGAACACATAAAGCTGTTTACGTAAGCAACGGAGATGAAGAAGCTGTTAAAGCATCTCAGATCCTTGATAACGATGATATCAAGGAAATCGAGCTTATCAACTCCGACAAACGAAGCTTAACCCCTGAAAACTCAATCGACTTTGCCTTCGTCTCTGCCTTCCCTGATGCCTCAAAGTTTATCGAAAGGACCCTTAAAATCCATGGGATTGTCGTGCTTTCGCTCAGCAATGACGCCTCTAACAGTTTCAAGAAGCCTAACAACTACAAGATGGTGTACCTGAGGCGATTTGACACGATCATTTTGGCAATGAGGAAGACCAGCACCCATGCAACCGAAACTTCATCCTCTACCACCACCTCTCCCACAAGGAGAAGGCTCTTGGCTGAGGAGGCAAAGAAGGAGGCGCTGAAGAAGCTCGAAGATGTGCTTCTAGAGCCCCCGAGAGCCTCATCTGGGAAATCGAGGAGGTACTTGAAGAGAACAAGGTACCTCCCGGACTTGATGGGTGACTCGCTCGAGAGCTACCCTAGAAGGGTTTTCATTGACGTAGGGTTGCCAGAGAAGGAAGGCGGAAGTGGGACGAGCTGGTTCGCAAAGAACTACCCTACCCGGGACAAGAACTTTGAGATGTACAAGATCGAGACTGTAACAGAGGATAGTTCCGGGAAGGAGGTGGTTCAGACCGGAATATCGGAGTGGCTGAACAAGAATGTTAAGGATGAGGAGTATGTGGTGATGAAGGCCGAGGCCGAGGTGGTGGAGGAGATGGTGAAGAGTAAAGCGATTCATTTGGTGGATGAGCTTTTCTTGGAGTGCAAGCACCAGGGACATAATCAAAAAATTAATGTTAGGAGCCGCAGGGCATATTGGCAGTGCTTAGCATTGTACGGACAGCTTCGGGACGAAGGTGTTGCGGTGCATCAATGGTGGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

413

Amino Acids

46.65

Weight (kDa)

8.77

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 237 - 411 5.7e-85 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 789
AccB1I GGYRCC 1 cut(s) 789
AccII CGCG 1 cut(s) 270
AciI CCGC 4 cut(s) 268, 873, 1159, 1217
AclWI GGATC 3 cut(s) 10, 115, 355
AcoI YGGCCR 1 cut(s) 276
AcuI CTGAAG 1 cut(s) 731
AcyI GRCGYC 1 cut(s) 534
AfaI GTAC 7 cut(s) 31, 86, 578, 773, 791, 935, 1190
AfiI CCNNNNNNNGG 4 cut(s) 274, 756, 835, 913
AgsI TTSAA 3 cut(s) 178, 550, 778
AjiI CACGTC 1 cut(s) 235
AjnI CCWGG 1 cut(s) 1125
AleI CACNNNNGTG 1 cut(s) 169
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 2 cut(s) 457, 938
AlwI GGATC 3 cut(s) 10, 115, 355
Ama87I CYCGRG 3 cut(s) 742, 818, 912
AoxI GGCC 3 cut(s) 276, 1044, 1050
ApeKI GCWGC 1 cut(s) 53
ArsI GACNNNNNNTTYG 4 cut(s) 431, 463, 908, 940
AseI ATTAAT 1 cut(s) 1146
Asp718I GGTACC 1 cut(s) 789
AspLEI GCGC 1 cut(s) 709
AspS9I GGNCC 1 cut(s) 489
AsuC2I CCSGG 4 cut(s) 797, 913, 914, 967
AsuHPI GGTGA 3 cut(s) 821, 1048, 1084
AvaI CYCGRG 3 cut(s) 742, 818, 912
AvaII GGWCC 1 cut(s) 489
AxyI CCTNAGG 1 cut(s) 581
BalI TGGCCA 1 cut(s) 278
BanI GGYRCC 1 cut(s) 789
BanII GRGCYC 2 cut(s) 227, 741
BbsI GAAGAC 1 cut(s) 623
Bbv12I GWGCWC 1 cut(s) 227
BbvCI CCTCAGC 1 cut(s) 687
BbvI GCAGC 1 cut(s) 40
BccI CCATC 3 cut(s) 565, 799, 1063
BcgI CGANNNNNNTGC 2 cut(s) 1196, 1230
BciT130I CCWGG 1 cut(s) 1127
BciVI GTATCC 1 cut(s) 81
BcnI CCSGG 4 cut(s) 797, 913, 914, 967
BcoDI GTCTC 2 cut(s) 457, 938
BfaI CTAG 2 cut(s) 734, 831
BfoI RGCGCY 1 cut(s) 710
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 2 cut(s) 54, 1159
BlpI GCTNAGC 2 cut(s) 524, 1180
BlsI GCNGC 2 cut(s) 55, 1160
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 5 cut(s) 797, 913, 914, 967, 1127
Bme18I GGWCC 1 cut(s) 489
BmeT110I CYCGRG 3 cut(s) 742, 818, 912
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 489
BmiI GGNNCC 3 cut(s) 491, 791, 1157
BmrFI CCNGG 5 cut(s) 797, 913, 914, 967, 1127
BmsI GCATC 5 cut(s) 67, 165, 362, 457, 1231
BpiI GAAGAC 1 cut(s) 623
BpmI CTGGAG 1 cut(s) 266
Bpu10I CCTNAGC 1 cut(s) 687
Bpu1102I GCTNAGC 2 cut(s) 524, 1180
BpuMI CCSGG 4 cut(s) 797, 913, 914, 967
BsaAI YACGTR 1 cut(s) 322
BsaHI GRCGYC 1 cut(s) 534
BsaJI CCNNGG 7 cut(s) 19, 170, 502, 912, 1047, 1053, 1126
BsaWI WCCGGW 1 cut(s) 986
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bsc4I CCNNNNNNNGG 4 cut(s) 274, 756, 835, 913
Bse21I CCTNAGG 1 cut(s) 581
Bse3DI GCAATG 2 cut(s) 535, 615
BseBI CCWGG 1 cut(s) 1127
BseDI CCNNGG 7 cut(s) 19, 170, 502, 912, 1047, 1053, 1126
BseGI GGATG 4 cut(s) 227, 644, 1027, 1105
BseLI CCNNNNNNNGG 4 cut(s) 274, 756, 835, 913
BseMI GCAATG 2 cut(s) 535, 615
BseMII CTCAG 4 cut(s) 371, 538, 572, 678
BseRI GAGGAG 4 cut(s) 704, 781, 1040, 1079
BseXI GCAGC 1 cut(s) 40
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 3 cut(s) 278, 1046, 1052
BshNI GGYRCC 1 cut(s) 789
BsiHKAI GWGCWC 1 cut(s) 227
BsiHKCI CYCGRG 3 cut(s) 742, 818, 912
BsiSI CCGG 4 cut(s) 797, 913, 966, 987
BslFI GGGAC 4 cut(s) 895, 929, 1143, 1217
BslI CCNNNNNNNGG 4 cut(s) 274, 756, 835, 913
BsmAI GTCTC 2 cut(s) 457, 938
BsmBI CGTCTC 1 cut(s) 457
BsmFI GGGAC 4 cut(s) 895, 929, 1143, 1217
BsnI GGCC 3 cut(s) 278, 1046, 1052
BsoBI CYCGRG 3 cut(s) 742, 818, 912
Bsp1286I GDGCHC 2 cut(s) 227, 741
Bsp1407I TGTACA 1 cut(s) 933
Bsp143I GATC 8 cut(s) 6, 15, 120, 141, 261, 360, 597, 939
Bsp1720I GCTNAGC 2 cut(s) 524, 1180
Bsp19I CCATGG 1 cut(s) 502
BspACI CCGC 4 cut(s) 268, 873, 1159, 1217
BspANI GGCC 3 cut(s) 278, 1046, 1052
BspCNI CTCAG 4 cut(s) 370, 537, 573, 679
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 3 cut(s) 491, 791, 1157
BspPI GGATC 3 cut(s) 10, 115, 355
BspT107I GGYRCC 1 cut(s) 789
BsrDI GCAATG 2 cut(s) 535, 615
BsrGI TGTACA 1 cut(s) 933
BssECI CCNNGG 7 cut(s) 19, 170, 502, 912, 1047, 1053, 1126
BssMI GATC 8 cut(s) 6, 15, 120, 141, 261, 360, 597, 939
BssNI GRCGYC 1 cut(s) 534
BssT1I CCWWGG 2 cut(s) 19, 502
Bst2UI CCWGG 1 cut(s) 1127
Bst4CI ACNGT 2 cut(s) 545, 949
Bst6I CTCTTC 2 cut(s) 773, 1070
BstACI GRCGYC 1 cut(s) 534
BstAUI TGTACA 1 cut(s) 933
BstBAI YACGTR 1 cut(s) 322
BstC8I GCNNGC 2 cut(s) 51, 1121
BstDEI CTNAG 5 cut(s) 357, 524, 581, 687, 1180
BstDSI CCRYGG 2 cut(s) 170, 502
BstF5I GGATG 4 cut(s) 227, 644, 1027, 1105
BstFNI CGCG 1 cut(s) 270
BstH2I RGCGCY 1 cut(s) 710
BstHHI GCGC 1 cut(s) 709
BstKTI GATC 8 cut(s) 9, 18, 123, 144, 264, 363, 600, 942
BstMAI GTCTC 2 cut(s) 457, 938
BstMBI GATC 8 cut(s) 6, 15, 120, 141, 261, 360, 597, 939
BstMWI GCNNNNNNNGC 4 cut(s) 350, 692, 715, 736
BstNI CCWGG 1 cut(s) 1127
BstNSI RCATGY 1 cut(s) 53
BstSCI CCNGG 5 cut(s) 795, 911, 912, 965, 1125
BstSNI TACGTA 1 cut(s) 322
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 40
BstV2I GAAGAC 1 cut(s) 623
BstX2I RGATCY 1 cut(s) 360
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 1 cut(s) 360
Bsu36I CCTNAGG 1 cut(s) 581
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 3 cut(s) 278, 1046, 1052
BtgI CCRYGG 2 cut(s) 170, 502
BtgZI GCGATG 1 cut(s) 142
BtrI CACGTC 1 cut(s) 235
BtsCI GGATG 4 cut(s) 227, 644, 1027, 1105
BtsI GCAGTG 1 cut(s) 1181
BtsIMutI CAGTG 1 cut(s) 1181
Cac8I GCNNGC 2 cut(s) 51, 1121
CfoI GCGC 1 cut(s) 709
Cfr13I GGNCC 1 cut(s) 489
Cfr9I CCCGGG 1 cut(s) 912
CseI GACGC 1 cut(s) 542
Csp6I GTAC 7 cut(s) 30, 85, 577, 772, 790, 934, 1189
CviAII CATG 3 cut(s) 50, 503, 629
CviQI GTAC 7 cut(s) 30, 85, 577, 772, 790, 934, 1189
DdeI CTNAG 5 cut(s) 357, 524, 581, 687, 1180
DpnI GATC 8 cut(s) 8, 17, 122, 143, 263, 362, 599, 941
DpnII GATC 8 cut(s) 6, 15, 120, 141, 261, 360, 597, 939
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 2 cut(s) 773, 1070
EarI CTCTTC 2 cut(s) 773, 1070
EciI GGCGGA 1 cut(s) 888
Ecl136II GAGCTC 1 cut(s) 225
Eco105I TACGTA 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 19, 502
Eco24I GRGCYC 2 cut(s) 227, 741
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 489
Eco53kI GAGCTC 1 cut(s) 225
Eco57I CTGAAG 1 cut(s) 731
Eco81I CCTNAGG 1 cut(s) 581
Eco88I CYCGRG 3 cut(s) 742, 818, 912
EcoICRI GAGCTC 1 cut(s) 225
EcoO109I RGGNCCY 1 cut(s) 489
EcoRII CCWGG 1 cut(s) 1125
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 19, 502
EcoT38I GRGCYC 2 cut(s) 227, 741
ErhI CCWWGG 2 cut(s) 19, 502
Esp3I CGTCTC 1 cut(s) 457
FaeI CATG 3 cut(s) 53, 506, 632
FaiI YATR 8 cut(s) 51, 292, 309, 504, 630, 1032, 1134, 1168
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 4 cut(s) 895, 929, 1143, 1217
FatI CATG 3 cut(s) 49, 502, 628
Fnu4HI GCNGC 2 cut(s) 54, 1159
FokI GGATG 4 cut(s) 214, 631, 1034, 1112
FriOI GRGCYC 2 cut(s) 227, 741
Fsp4HI GCNGC 2 cut(s) 54, 1159
FspBI CTAG 2 cut(s) 734, 831
GlaI GCGC 1 cut(s) 708
GluI GCNGC 2 cut(s) 54, 1159
GsuI CTGGAG 1 cut(s) 266
HaeII RGCGCY 1 cut(s) 710
HaeIII GGCC 3 cut(s) 278, 1046, 1052
HapII CCGG 4 cut(s) 797, 913, 966, 987
HgaI GACGC 1 cut(s) 542
HhaI GCGC 1 cut(s) 709
Hin1I GRCGYC 1 cut(s) 534
Hin1II CATG 3 cut(s) 53, 506, 632
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HindIII AAGCTT 1 cut(s) 415
HinfI GANTC 3 cut(s) 100, 812, 1087
HpaII CCGG 4 cut(s) 797, 913, 966, 987
HphI GGTGA 3 cut(s) 821, 1048, 1084
Hpy166II GTNNAC 2 cut(s) 319, 577
Hpy188I TCNGA 7 cut(s) 65, 99, 232, 360, 406, 984, 997
Hpy188III TCNNGA 8 cut(s) 74, 111, 245, 550, 734, 820, 943, 1202
Hpy8I GTNNAC 2 cut(s) 319, 577
Hpy99I CGWCG 1 cut(s) 236
HpyCH4III ACNGT 2 cut(s) 545, 949
HpyCH4IV ACGT 3 cut(s) 234, 321, 849
HpyCH4V TGCA 4 cut(s) 156, 632, 1119, 1222
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 692, 715, 736
HpyF3I CTNAG 5 cut(s) 357, 524, 581, 687, 1180
HpySE526I ACGT 3 cut(s) 234, 321, 849
Hsp92I GRCGYC 1 cut(s) 534
Hsp92II CATG 3 cut(s) 53, 506, 632
HspAI GCGC 1 cut(s) 707
KpnI GGTACC 1 cut(s) 793
Kzo9I GATC 8 cut(s) 6, 15, 120, 141, 261, 360, 597, 939
LmnI GCTCC 3 cut(s) 61, 247, 1155
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 5 cut(s) 67, 165, 362, 457, 1231
MaeI CTAG 2 cut(s) 734, 831
MaeII ACGT 3 cut(s) 234, 321, 849
MaeIII GTNAC 2 cut(s) 809, 949
MalI GATC 8 cut(s) 8, 17, 122, 143, 263, 362, 599, 941
MboI GATC 8 cut(s) 6, 15, 120, 141, 261, 360, 597, 939
MboII GAAGA 7 cut(s) 256, 350, 628, 724, 734, 790, 1087
MflI RGATCY 1 cut(s) 360
MhlI GDGCHC 2 cut(s) 227, 741
MlsI TGGCCA 1 cut(s) 278
MluCI AATT 3 cut(s) 10, 106, 1143
MluNI TGGCCA 1 cut(s) 278
MlyI GAGTC 1 cut(s) 806
MmeI TCCRAC 2 cut(s) 255, 429
Mox20I TGGCCA 1 cut(s) 278
MscI TGGCCA 1 cut(s) 278
MseI TTAA 6 cut(s) 240, 348, 419, 495, 1017, 1146
MslI CAYNNNNRTG 1 cut(s) 169
Msp20I TGGCCA 1 cut(s) 278
MspI CCGG 4 cut(s) 797, 913, 966, 987
MspR9I CCNGG 5 cut(s) 797, 913, 914, 967, 1127
MvaI CCWGG 1 cut(s) 1127
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 4 cut(s) 350, 692, 715, 736
NciI CCSGG 4 cut(s) 797, 913, 914, 967
NcoI CCATGG 1 cut(s) 502
NdeII GATC 8 cut(s) 6, 15, 120, 141, 261, 360, 597, 939
NlaIII CATG 3 cut(s) 53, 506, 632
NlaIV GGNNCC 3 cut(s) 491, 791, 1157
NmeAIII GCCGAG 2 cut(s) 1072, 1078
NmuCI GTSAC 1 cut(s) 809
NspI RCATGY 1 cut(s) 53
OliI CACNNNNGTG 1 cut(s) 169
PaeI GCATGC 1 cut(s) 53
PaeR7I CTCGAG 1 cut(s) 818
PfeI GAWTC 2 cut(s) 100, 1087
PfoI TCCNGGA 2 cut(s) 795, 965
PkrI GCNGC 2 cut(s) 55, 1160
PleI GAGTC 1 cut(s) 806
PpsI GAGTC 1 cut(s) 806
Ppu21I YACGTR 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 489
PshBI ATTAAT 1 cut(s) 1146
Psp124BI GAGCTC 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 1125
PspGI CCWGG 1 cut(s) 1125
PspN4I GGNNCC 3 cut(s) 491, 791, 1157
PspPI GGNCC 1 cut(s) 489
PspPPI RGGWCCY 1 cut(s) 489
PsuI RGATCY 1 cut(s) 360
RsaI GTAC 7 cut(s) 31, 86, 578, 773, 791, 935, 1190
RsaNI GTAC 7 cut(s) 30, 85, 577, 772, 790, 934, 1189
RseI CAYNNNNRTG 1 cut(s) 169
SacI GAGCTC 1 cut(s) 227
SaqAI TTAA 6 cut(s) 240, 348, 419, 495, 1017, 1146
SatI GCNGC 2 cut(s) 54, 1159
Sau3AI GATC 8 cut(s) 6, 15, 120, 141, 261, 360, 597, 939
Sau96I GGNCC 1 cut(s) 489
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 1 cut(s) 806
ScrFI CCNGG 5 cut(s) 797, 913, 914, 967, 1127
SduI GDGCHC 2 cut(s) 227, 741
SfaNI GCATC 5 cut(s) 67, 165, 362, 457, 1231
Sfr274I CTCGAG 1 cut(s) 818
SinI GGWCC 1 cut(s) 489
SlaI CTCGAG 1 cut(s) 818
SmaI CCCGGG 1 cut(s) 914
SmiMI CAYNNNNRTG 1 cut(s) 169
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
SnaBI TACGTA 1 cut(s) 322
SphI GCATGC 1 cut(s) 53
Sse9I AATT 3 cut(s) 10, 106, 1143
SsiI CCGC 4 cut(s) 268, 873, 1159, 1217
SspMI CTAG 2 cut(s) 734, 831
SstI GAGCTC 1 cut(s) 227
StyD4I CCNGG 5 cut(s) 795, 911, 912, 965, 1125
StyI CCWWGG 2 cut(s) 19, 502
TaaI ACNGT 2 cut(s) 545, 949
TaiI ACGT 3 cut(s) 237, 324, 852
TaqI TCGA 7 cut(s) 390, 438, 483, 720, 764, 819, 942
TasI AATT 3 cut(s) 10, 106, 1143
TatI WGTACW 2 cut(s) 84, 933
TauI GCSGC 1 cut(s) 1161
TfiI GAWTC 2 cut(s) 100, 1087
Tru1I TTAA 6 cut(s) 240, 348, 419, 495, 1017, 1146
Tru9I TTAA 6 cut(s) 240, 348, 419, 495, 1017, 1146
TscAI CASTG 1 cut(s) 1181
TseFI GTSAC 1 cut(s) 809
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 809
TspDTI ATGAA 5 cut(s) 351, 633, 832, 1055, 1079
TspGWI ACGGA 3 cut(s) 17, 345, 1206
TspMI CCCGGG 1 cut(s) 912
TspRI CASTG 1 cut(s) 1181
VpaK11BI GGWCC 1 cut(s) 489
VspI ATTAAT 1 cut(s) 1146
XbaI TCTAGA 1 cut(s) 733
XceI RCATGY 1 cut(s) 53
XhoI CTCGAG 1 cut(s) 818
XmaI CCCGGG 1 cut(s) 912
XspI CTAG 2 cut(s) 734, 831
ZrmI AGTACT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.