Prupe.1G529700_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011711
Physical Location & Seq
Forward (+)
43309502 .. 43311375
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G529700.1

Sequence Viewer

Length: 1251 bp
ATGGCCATCAACTTGGTCCAAGGTTTCCGCACCAAGGCCCACCCGAAACACGCTGCTTCGATCGGGTTGTACCCGGATGCCAAGTACTTGGTTATTCGGATCCCAGACTCTAGAGCATTGATGATCCTTAGCCGTTCTTTGATTCTGGCACTCTGCATAGTCTCGCTGCCGTGTTTGAATTCCCTCCTTTCAATCGCAACCACTTCTTCTCCTGAAATAACTACCTCTGACGTCATGATCCCCGTTAAAATCGGGCTCCTGCCTTTGATCTTCCGCGACTTGGCCAACGAAGGCCTGTTCAAGAGAGGCACGCATAAGGCTGTTTTCGTGAGCGGCCGCCGAGACGAAGAGGCTGTCAAGAAATCAGAGATCCTCGACGTTAATGAGATGGAGCTTATCGACGCTGAAAAACAAAGCTCAATCCCTGACAACTCAATTGACTTCGCCTTCGTCTCTTCCTTCCCCACCGCCTCTAAATTCCTCGAAAGGACCCTTAAAGTTGGCGGGATCGTCGCGCTTCAGCTTAGCGACAACCCCGCCAGCGGCTTCAAGAAGCCTGAGAACTACAAGATGGTGTACTTGAGGCGGTTCGACGCGACCGTTTTGGCCATGAGGAAGACCGGAAAGGCATCCGCAACGACTTCTCCCACCCGGAGACGGCTCTTCGCCGAGGAGGCAAAGAAGGAAGCCTTGAAGAATCTAGAAGACGTGCTTCTCGAACCCCCAAGAGCCTCGTCCTTGAAATCGAGAAGGTACTTGAAGCGAACAAGGTATCTCCCGGATTTGATGGGTGACTCTCTCGAGAGCTACCCTCGAAGGGTGTTCATAGACGTAGGATTGCCGGAGAAGGAAGGCGGGAGCGGGACAGGCTGGTTCGCGAAGAACTACCCGACCCGGAACAAGAACTTTGAGATGTACAAGATCGAGACGGTGACGGAGGAGAGCTCCGGGAAGGAGGTGGTTCAGACCGGGATGTCGGAGTGGCTGAAGAAGAACGTGAAGGAGGAGGAGTACGTGGTGATGAAGGCGGAGGCCGAGGTAGTGGAGGAGATGATGAACACCAAAGCGATCAAGTTGGTGGATGAGCTTTTCTTGGAGTGCAAGCCACAGGGGCAAAATGGGAATAAGGGTGATAATAATAGTAATAGTAAGACTAACTATGTGAGGAGTCGCAGGGCTTACTGGCAGTGCTTGGCTTTGTACGGACAGCTTCGGGACGAAGGTGTTGCTGTGCATCAATGGTGGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

417

Amino Acids

46.8

Weight (kDa)

9.2

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 973
AatII GACGTC 1 cut(s) 234
AccBSI CCGCTC 2 cut(s) 333, 861
AccII CGCG 4 cut(s) 276, 515, 596, 878
AclWI GGATC 6 cut(s) 94, 107, 118, 232, 364, 515
AcoI YGGCCR 4 cut(s) 3, 282, 334, 606
AcsI RAATTY 2 cut(s) 178, 476
AcuI CTGAAG 2 cut(s) 503, 1007
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 7 cut(s) 71, 86, 578, 755, 917, 1013, 1202
AfiI CCNNNNNNNGG 5 cut(s) 34, 280, 542, 657, 817
AgsI TTSAA 7 cut(s) 178, 192, 301, 550, 694, 742, 760
AjiI CACGTC 1 cut(s) 709
AluBI AGCT 7 cut(s) 394, 417, 523, 807, 945, 1087, 1210
AluI AGCT 7 cut(s) 394, 417, 523, 807, 945, 1087, 1210
Alw21I GWGCWC 1 cut(s) 947
Alw26I GTCTC 5 cut(s) 166, 336, 457, 649, 920
AlwI GGATC 6 cut(s) 94, 107, 118, 232, 364, 515
Ama87I CYCGRG 1 cut(s) 800
AoxI GGCC 7 cut(s) 3, 36, 282, 292, 334, 606, 1032
ApeKI GCWGC 2 cut(s) 53, 166
ApoI RAATTY 2 cut(s) 178, 476
ArsI GACNNNNNNTTYG 4 cut(s) 339, 371, 431, 463
AspLEI GCGC 1 cut(s) 517
AspS9I GGNCC 3 cut(s) 16, 37, 489
AsuC2I CCSGG 6 cut(s) 74, 652, 779, 895, 949, 970
AsuHPI GGTGA 4 cut(s) 803, 943, 1030, 1142
AvaI CYCGRG 1 cut(s) 800
AvaII GGWCC 2 cut(s) 16, 489
BalI TGGCCA 3 cut(s) 5, 284, 608
BamHI GGATCC 1 cut(s) 99
BanII GRGCYC 2 cut(s) 258, 947
BbsI GAAGAC 2 cut(s) 623, 711
Bbv12I GWGCWC 1 cut(s) 947
BbvI GCAGC 2 cut(s) 40, 153
BccI CCATC 4 cut(s) 14, 382, 565, 781
BceAI ACGGC 3 cut(s) 117, 154, 674
BcgI CGANNNNNNTGC 2 cut(s) 1208, 1242
BcnI CCSGG 6 cut(s) 74, 652, 779, 895, 949, 970
BcoDI GTCTC 5 cut(s) 166, 336, 457, 649, 920
BfaI CTAG 2 cut(s) 111, 701
BglI GCCNNNNNGGC 2 cut(s) 674, 1111
BisI GCNGC 5 cut(s) 54, 167, 334, 337, 544
BlpI GCTNAGC 1 cut(s) 524
BlsI GCNGC 5 cut(s) 55, 168, 335, 338, 545
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 6 cut(s) 74, 652, 779, 895, 949, 970
Bme18I GGWCC 2 cut(s) 16, 489
BmeT110I CYCGRG 1 cut(s) 800
BmgBI CACGTC 1 cut(s) 709
BmgT120I GGNCC 3 cut(s) 16, 37, 489
BmiI GGNNCC 3 cut(s) 101, 257, 491
BmrFI CCNGG 6 cut(s) 74, 652, 779, 895, 949, 970
BmsI GCATC 3 cut(s) 67, 638, 1243
BpiI GAAGAC 2 cut(s) 623, 711
BplI GAGNNNNNCTC 4 cut(s) 796, 828, 929, 961
Bpu10I CCTNAGC 1 cut(s) 128
Bpu1102I GCTNAGC 1 cut(s) 524
BpuEI CTTGAG 1 cut(s) 601
BpuMI CCSGG 6 cut(s) 74, 652, 779, 895, 949, 970
BsaAI YACGTR 1 cut(s) 1015
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 4 cut(s) 19, 33, 669, 1035
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 6 cut(s) 193, 223, 628, 658, 995, 1025
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 280, 542, 657, 817
Bse1I ACTGG 1 cut(s) 1187
BseDI CCNNGG 4 cut(s) 19, 33, 669, 1035
BseGI GGATG 4 cut(s) 82, 629, 978, 1087
BseLI CCNNNNNNNGG 5 cut(s) 34, 280, 542, 657, 817
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 1 cut(s) 1187
BseRI GAGGAG 6 cut(s) 686, 953, 1019, 1022, 1061, 1180
BseX3I CGGCCG 1 cut(s) 334
BseXI GCAGC 2 cut(s) 40, 153
Bsh1236I CGCG 4 cut(s) 276, 515, 596, 878
Bsh1285I CGRYCG 3 cut(s) 63, 337, 600
BshFI GGCC 7 cut(s) 5, 38, 284, 294, 336, 608, 1034
BsiEI CGRYCG 3 cut(s) 63, 337, 600
BsiHKAI GWGCWC 1 cut(s) 947
BsiHKCI CYCGRG 1 cut(s) 800
BsiSI CCGG 8 cut(s) 74, 621, 652, 779, 842, 895, 948, 969
BslFI GGGAC 2 cut(s) 877, 1229
BslI CCNNNNNNNGG 5 cut(s) 34, 280, 542, 657, 817
BsmAI GTCTC 5 cut(s) 166, 336, 457, 649, 920
BsmBI CGTCTC 4 cut(s) 336, 457, 649, 920
BsmFI GGGAC 2 cut(s) 877, 1229
BsnI GGCC 7 cut(s) 5, 38, 284, 294, 336, 608, 1034
BsoBI CYCGRG 1 cut(s) 800
Bsp1286I GDGCHC 2 cut(s) 258, 947
Bsp1407I TGTACA 1 cut(s) 915
Bsp143I GATC 9 cut(s) 60, 99, 123, 237, 267, 369, 507, 921, 1068
Bsp1720I GCTNAGC 1 cut(s) 524
Bsp68I TCGCGA 1 cut(s) 878
BspANI GGCC 7 cut(s) 5, 38, 284, 294, 336, 608, 1034
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 4 cut(s) 276, 515, 596, 878
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 3 cut(s) 101, 257, 491
BspPI GGATC 6 cut(s) 94, 107, 118, 232, 364, 515
BspQI GCTCTTC 1 cut(s) 668
BsrBI CCGCTC 2 cut(s) 333, 861
BsrGI TGTACA 1 cut(s) 915
BsrI ACTGG 1 cut(s) 1187
BssECI CCNNGG 4 cut(s) 19, 33, 669, 1035
BssMI GATC 9 cut(s) 60, 99, 123, 237, 267, 369, 507, 921, 1068
BssNI GRCGYC 1 cut(s) 231
BssT1I CCWWGG 2 cut(s) 19, 33
Bst4CI ACNGT 2 cut(s) 601, 931
Bst6I CTCTTC 3 cut(s) 342, 460, 668
BstACI GRCGYC 1 cut(s) 231
BstAUI TGTACA 1 cut(s) 915
BstBAI YACGTR 1 cut(s) 1015
BstC8I GCNNGC 3 cut(s) 311, 541, 1103
BstDEI CTNAG 3 cut(s) 128, 524, 558
BstF5I GGATG 4 cut(s) 82, 629, 978, 1087
BstFNI CGCG 4 cut(s) 276, 515, 596, 878
BstHHI GCGC 1 cut(s) 517
BstKTI GATC 9 cut(s) 63, 102, 126, 240, 270, 372, 510, 924, 1071
BstMAI GTCTC 5 cut(s) 166, 336, 457, 649, 920
BstMBI GATC 9 cut(s) 60, 99, 123, 237, 267, 369, 507, 921, 1068
BstMCI CGRYCG 3 cut(s) 63, 337, 600
BstMWI GCNNNNNNNGC 3 cut(s) 674, 867, 1111
BstSCI CCNGG 6 cut(s) 72, 650, 777, 893, 947, 968
BstUI CGCG 4 cut(s) 276, 515, 596, 878
BstV1I GCAGC 2 cut(s) 40, 153
BstV2I GAAGAC 2 cut(s) 623, 711
BstX2I RGATCY 2 cut(s) 99, 369
BstXI CCANNNNNNTGG 2 cut(s) 13, 88
BstYI RGATCY 2 cut(s) 99, 369
BstZI CGGCCG 1 cut(s) 334
BsuRI GGCC 7 cut(s) 5, 38, 284, 294, 336, 608, 1034
BtrI CACGTC 1 cut(s) 709
BtsCI GGATG 4 cut(s) 82, 629, 978, 1087
BtsI GCAGTG 1 cut(s) 1193
BtsIMutI CAGTG 1 cut(s) 1193
BtuMI TCGCGA 1 cut(s) 878
Cac8I GCNNGC 3 cut(s) 311, 541, 1103
CciI TCATGA 1 cut(s) 234
CciNI GCGGCCGC 1 cut(s) 334
CfoI GCGC 1 cut(s) 517
Cfr13I GGNCC 3 cut(s) 16, 37, 489
CseI GACGC 2 cut(s) 410, 602
Csp6I GTAC 7 cut(s) 70, 85, 577, 754, 916, 1012, 1201
CviAII CATG 2 cut(s) 235, 610
CviQI GTAC 7 cut(s) 70, 85, 577, 754, 916, 1012, 1201
DdeI CTNAG 3 cut(s) 128, 524, 558
DpnI GATC 9 cut(s) 62, 101, 125, 239, 269, 371, 509, 923, 1070
DpnII GATC 9 cut(s) 60, 99, 123, 237, 267, 369, 507, 921, 1068
DrdI GACNNNNNNGTC 1 cut(s) 973
DseDI GACNNNNNNGTC 1 cut(s) 973
EaeI YGGCCR 4 cut(s) 3, 282, 334, 606
EagI CGGCCG 1 cut(s) 334
Eam1104I CTCTTC 3 cut(s) 342, 460, 668
EarI CTCTTC 3 cut(s) 342, 460, 668
EciI GGCGGA 1 cut(s) 1043
Ecl136II GAGCTC 1 cut(s) 945
EclXI CGGCCG 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 19, 33
Eco147I AGGCCT 1 cut(s) 294
Eco24I GRGCYC 2 cut(s) 258, 947
Eco47I GGWCC 2 cut(s) 16, 489
Eco52I CGGCCG 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 945
Eco57I CTGAAG 2 cut(s) 503, 1007
Eco88I CYCGRG 1 cut(s) 800
EcoICRI GAGCTC 1 cut(s) 945
EcoO109I RGGNCCY 1 cut(s) 489
EcoRI GAATTC 1 cut(s) 178
EcoT14I CCWWGG 2 cut(s) 19, 33
EcoT38I GRGCYC 2 cut(s) 258, 947
ErhI CCWWGG 2 cut(s) 19, 33
Esp3I CGTCTC 4 cut(s) 336, 457, 649, 920
FaeI CATG 2 cut(s) 238, 613
FaiI YATR 6 cut(s) 158, 236, 315, 611, 827, 1161
FalI AAGNNNNNCTT 4 cut(s) 674, 706, 696, 728
FaqI GGGAC 2 cut(s) 877, 1229
FatI CATG 2 cut(s) 234, 609
FauI CCCGC 4 cut(s) 497, 544, 848, 854
Fnu4HI GCNGC 5 cut(s) 54, 167, 334, 337, 544
FokI GGATG 4 cut(s) 89, 616, 985, 1094
FriOI GRGCYC 2 cut(s) 258, 947
Fsp4HI GCNGC 5 cut(s) 54, 167, 334, 337, 544
FspBI CTAG 2 cut(s) 111, 701
GlaI GCGC 1 cut(s) 516
GluI GCNGC 5 cut(s) 54, 167, 334, 337, 544
HaeIII GGCC 7 cut(s) 5, 38, 284, 294, 336, 608, 1034
HapII CCGG 8 cut(s) 74, 621, 652, 779, 842, 895, 948, 969
HgaI GACGC 2 cut(s) 410, 602
HhaI GCGC 1 cut(s) 517
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 2 cut(s) 238, 613
Hin6I GCGC 1 cut(s) 515
HinP1I GCGC 1 cut(s) 515
HinfI GANTC 5 cut(s) 107, 142, 697, 794, 1168
HpaII CCGG 8 cut(s) 74, 621, 652, 779, 842, 895, 948, 969
HphI GGTGA 4 cut(s) 803, 943, 1030, 1142
Hpy166II GTNNAC 1 cut(s) 577
Hpy188I TCNGA 5 cut(s) 99, 229, 367, 966, 979
Hpy8I GTNNAC 1 cut(s) 577
Hpy99I CGWCG 4 cut(s) 380, 404, 515, 596
HpyCH4III ACNGT 2 cut(s) 601, 931
HpyCH4IV ACGT 6 cut(s) 231, 378, 708, 831, 996, 1014
HpyCH4V TGCA 3 cut(s) 156, 1101, 1234
HpyF10VI GCNNNNNNNGC 3 cut(s) 674, 867, 1111
HpyF3I CTNAG 3 cut(s) 128, 524, 558
HpySE526I ACGT 6 cut(s) 231, 378, 708, 831, 996, 1014
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 2 cut(s) 238, 613
HspAI GCGC 1 cut(s) 515
Kzo9I GATC 9 cut(s) 60, 99, 123, 237, 267, 369, 507, 921, 1068
LguI GCTCTTC 1 cut(s) 668
LmnI GCTCC 4 cut(s) 261, 391, 858, 950
Lsp1109I GCAGC 2 cut(s) 40, 153
LweI GCATC 3 cut(s) 67, 638, 1243
MaeI CTAG 2 cut(s) 111, 701
MaeII ACGT 6 cut(s) 231, 378, 708, 831, 996, 1014
MaeIII GTNAC 2 cut(s) 791, 931
MalI GATC 9 cut(s) 62, 101, 125, 239, 269, 371, 509, 923, 1070
MbiI CCGCTC 2 cut(s) 333, 861
MboI GATC 9 cut(s) 60, 99, 123, 237, 267, 369, 507, 921, 1068
MfeI CAATTG 1 cut(s) 435
MflI RGATCY 2 cut(s) 99, 369
MhlI GDGCHC 2 cut(s) 258, 947
MlsI TGGCCA 3 cut(s) 5, 284, 608
MluCI AATT 3 cut(s) 178, 435, 476
MluNI TGGCCA 3 cut(s) 5, 284, 608
MlyI GAGTC 3 cut(s) 101, 788, 1177
MmeI TCCRAC 1 cut(s) 957
Mox20I TGGCCA 3 cut(s) 5, 284, 608
MscI TGGCCA 3 cut(s) 5, 284, 608
MseI TTAA 3 cut(s) 246, 381, 495
Msp20I TGGCCA 3 cut(s) 5, 284, 608
MspA1I CMGCKG 1 cut(s) 543
MspI CCGG 8 cut(s) 74, 621, 652, 779, 842, 895, 948, 969
MspR9I CCNGG 6 cut(s) 74, 652, 779, 895, 949, 970
MunI CAATTG 1 cut(s) 435
MvnI CGCG 4 cut(s) 276, 515, 596, 878
MwoI GCNNNNNNNGC 3 cut(s) 674, 867, 1111
NciI CCSGG 6 cut(s) 74, 652, 779, 895, 949, 970
NdeII GATC 9 cut(s) 60, 99, 123, 237, 267, 369, 507, 921, 1068
NlaIII CATG 2 cut(s) 238, 613
NlaIV GGNNCC 3 cut(s) 101, 257, 491
NmeAIII GCCGAG 3 cut(s) 365, 694, 1060
NmuCI GTSAC 2 cut(s) 791, 931
NotI GCGGCCGC 1 cut(s) 334
NruI TCGCGA 1 cut(s) 878
PaeR7I CTCGAG 1 cut(s) 800
PagI TCATGA 1 cut(s) 234
PceI AGGCCT 1 cut(s) 294
PciSI GCTCTTC 1 cut(s) 668
PcsI WCGNNNNNNNCGW 2 cut(s) 597, 714
PfeI GAWTC 2 cut(s) 142, 697
PfoI TCCNGGA 2 cut(s) 777, 947
PkrI GCNGC 5 cut(s) 55, 168, 335, 338, 545
Ple19I CGATCG 1 cut(s) 63
PleI GAGTC 3 cut(s) 101, 788, 1176
PpsI GAGTC 3 cut(s) 101, 788, 1176
Ppu21I YACGTR 1 cut(s) 1015
PpuMI RGGWCCY 1 cut(s) 489
Psp124BI GAGCTC 1 cut(s) 947
Psp5II RGGWCCY 1 cut(s) 489
PspN4I GGNNCC 3 cut(s) 101, 257, 491
PspPI GGNCC 3 cut(s) 16, 37, 489
PspPPI RGGWCCY 1 cut(s) 489
PsuI RGATCY 2 cut(s) 99, 369
PvuI CGATCG 1 cut(s) 63
RruI TCGCGA 1 cut(s) 878
RsaI GTAC 7 cut(s) 71, 86, 578, 755, 917, 1013, 1202
RsaNI GTAC 7 cut(s) 70, 85, 577, 754, 916, 1012, 1201
SacI GAGCTC 1 cut(s) 947
SapI GCTCTTC 1 cut(s) 668
SaqAI TTAA 3 cut(s) 246, 381, 495
SatI GCNGC 5 cut(s) 54, 167, 334, 337, 544
Sau3AI GATC 9 cut(s) 60, 99, 123, 237, 267, 369, 507, 921, 1068
Sau96I GGNCC 3 cut(s) 16, 37, 489
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 3 cut(s) 101, 788, 1177
ScrFI CCNGG 6 cut(s) 74, 652, 779, 895, 949, 970
SduI GDGCHC 2 cut(s) 258, 947
SfaNI GCATC 3 cut(s) 67, 638, 1243
Sfr274I CTCGAG 1 cut(s) 800
SinI GGWCC 2 cut(s) 16, 489
SlaI CTCGAG 1 cut(s) 800
SmlI CTYRAG 2 cut(s) 580, 800
SmoI CTYRAG 2 cut(s) 580, 800
Sse9I AATT 3 cut(s) 178, 435, 476
SseBI AGGCCT 1 cut(s) 294
SspMI CTAG 2 cut(s) 111, 701
SstI GAGCTC 1 cut(s) 947
StuI AGGCCT 1 cut(s) 294
StyD4I CCNGG 6 cut(s) 72, 650, 777, 893, 947, 968
StyI CCWWGG 2 cut(s) 19, 33
TaaI ACNGT 2 cut(s) 601, 931
TaiI ACGT 6 cut(s) 234, 381, 711, 834, 999, 1017
TasI AATT 3 cut(s) 178, 435, 476
TatI WGTACW 3 cut(s) 84, 576, 915
TauI GCSGC 3 cut(s) 336, 339, 546
TfiI GAWTC 2 cut(s) 142, 697
Tru1I TTAA 3 cut(s) 246, 381, 495
Tru9I TTAA 3 cut(s) 246, 381, 495
TscAI CASTG 1 cut(s) 1193
TseFI GTSAC 2 cut(s) 791, 931
TseI GCWGC 2 cut(s) 53, 166
Tsp45I GTSAC 2 cut(s) 791, 931
TspDTI ATGAA 3 cut(s) 814, 1037, 1070
TspGWI ACGGA 2 cut(s) 950, 1218
TspRI CASTG 1 cut(s) 1193
VpaK11BI GGWCC 2 cut(s) 16, 489
XapI RAATTY 2 cut(s) 178, 476
XbaI TCTAGA 2 cut(s) 110, 700
XhoI CTCGAG 1 cut(s) 800
XspI CTAG 2 cut(s) 111, 701
ZraI GACGTC 1 cut(s) 232
ZrmI AGTACT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.