Rmu_co8465559.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8465559.1
OG0011711
Physical Location & Seq
Forward (+)
139 .. 1362
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8465559.1_g000001.1.cds

Sequence Viewer

Length: 1224 bp
atggcaatcaatttggtccagggcttccgaaccaaacaacacctggcacacaaagccaccatcggattctaccccgatgtcaagtacttagtttttaggatccctgactcccgagtcttgttgtttctggctcgatgttccatcatggctttcgtgatcgtctccgttccgtggttgaagtcacttgttcccacaacagccaccgccaatggaatcatcggcttccccgcggaccctaatatcgagcttgtgccactcatcttccgcgacttggccaacgaaggcctcataaaaacccgtcatggcgagaaggcggtgtttgtcagcagtggcaatgatgaagaagccattcaaagctcacagatcctcaaagacaacgaaatggatctcatttccgtgaacgacgaaaatcaacagaactcgatcaacgaccaatctgttgacttcgccttcgtcttcgtctccgtcgtcccttccgccatgaagttcatcgaaaggactctcaaggttggcggcattgttgcgctccaggacctctccgccaggtttcacatgccttctaactacaagatggtctacctccgaaaactcgacgccaccgtggtggccatgaggaagatgagttcatcgatttcaactactacttctttcacgcgccgacgcctatttgctgaggaggcaaagaaggaagcattgaagaatctcgaagatgtgctgctcgaacccccacgtgcttcatcgggcaagtcgagccgatacttgaagaaaactcgctacctacccgacttgatgggagactcgctcgagggataccctcgcagggtcttcatcgacgttggcttgcctgaaaaggaaggcggtagtggcaccagctggttcgccaagaactacccgactcgcaacaagaattttgagatgtacaagattgagacaaccctgatcgacgagtccggcaagggggttcttcagaccggtatgtcggggtggttggagaagaatgttaaggaggaagagtatgttgtgatgaaggccgaggccgaagtggtggaggaaatgatgcagggtaaggtgatgaacttgatcgatgagcttttcttggaatgcaagccccaagggcacaatgggaaaggtaatttgagcactcgtagggcttactggcagtgcttggctttgtacggacagctcagggatgaaggcgttgcagtgcaccaatggtggggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

407

Amino Acids

45.87

Weight (kDa)

8.22

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 113, 976
AccB1I GGYRCC 1 cut(s) 866
AccI GTMKAC 1 cut(s) 576
AccII CGCG 3 cut(s) 230, 267, 655
AciI CCGC 9 cut(s) 204, 228, 230, 265, 314, 477, 513, 540, 858
AclWI GGATC 4 cut(s) 94, 107, 358, 393
AcoI YGGCCR 2 cut(s) 273, 606
AcsI RAATTY 1 cut(s) 907
AcuI CTGAAG 1 cut(s) 950
AcvI CACGTG 1 cut(s) 731
AcyI GRCGYC 2 cut(s) 594, 661
AfaI GTAC 3 cut(s) 86, 920, 1175
AfiI CCNNNNNNNGG 5 cut(s) 171, 271, 820, 957, 1216
AgeI ACCGGT 1 cut(s) 971
AgsI TTSAA 5 cut(s) 178, 353, 636, 697, 763
AjnI CCWGG 4 cut(s) 18, 42, 528, 542
AleI CACNNNNGTG 1 cut(s) 602
AloI GAACNNNNNNTCC 2 cut(s) 607, 639
AluBI AGCT 5 cut(s) 247, 357, 873, 1090, 1183
AluI AGCT 5 cut(s) 247, 357, 873, 1090, 1183
Alw21I GWGCWC 2 cut(s) 1142, 1209
Alw26I GTCTC 4 cut(s) 166, 466, 789, 923
Alw44I GTGCAC 1 cut(s) 1205
AlwI GGATC 4 cut(s) 94, 107, 358, 393
Ama87I CYCGRG 2 cut(s) 111, 803
AoxI GGCC 5 cut(s) 273, 283, 606, 1029, 1035
ApaLI GTGCAC 1 cut(s) 1205
ApeKI GCWGC 1 cut(s) 715
ApoI RAATTY 1 cut(s) 907
ArsI GACNNNNNNTTYG 2 cut(s) 434, 466
AsiGI ACCGGT 1 cut(s) 971
Asp700I GAANNNNTTC 1 cut(s) 348
AspLEI GCGC 2 cut(s) 526, 657
AspS9I GGNCC 3 cut(s) 16, 232, 532
AsuHPI GGTGA 1 cut(s) 1081
AvaI CYCGRG 2 cut(s) 111, 803
AvaII GGWCC 3 cut(s) 16, 232, 532
BaeGI GKGCMC 2 cut(s) 1119, 1209
BalI TGGCCA 2 cut(s) 275, 608
BamHI GGATCC 1 cut(s) 99
BanI GGYRCC 1 cut(s) 866
BbrPI CACGTG 1 cut(s) 731
BbsI GAAGAC 2 cut(s) 448, 817
Bbv12I GWGCWC 2 cut(s) 1142, 1209
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 1 cut(s) 702
BccI CCATC 4 cut(s) 68, 149, 565, 784
BciT130I CCWGG 4 cut(s) 20, 44, 530, 544
BciVI GTATCC 1 cut(s) 803
BcoDI GTCTC 4 cut(s) 166, 466, 789, 923
BfuI GTATCC 1 cut(s) 803
BglI GCCNNNNNGGC 1 cut(s) 1114
BisI GCNGC 2 cut(s) 514, 716
BlsI GCNGC 2 cut(s) 515, 717
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 4 cut(s) 20, 44, 530, 544
Bme18I GGWCC 3 cut(s) 16, 232, 532
BmeT110I CYCGRG 2 cut(s) 111, 803
BmgT120I GGNCC 3 cut(s) 16, 232, 532
BmiI GGNNCC 3 cut(s) 101, 234, 868
BmrFI CCNGG 4 cut(s) 20, 44, 530, 544
BmsI GCATC 1 cut(s) 1047
BpiI GAAGAC 2 cut(s) 448, 817
BplI GAGNNNNNCTC 2 cut(s) 786, 818
BpmI CTGGAG 1 cut(s) 512
Bpu10I CCTNAGC 2 cut(s) 672, 1184
BpuEI CTTGAG 1 cut(s) 488
Bsa29I ATCGAT 2 cut(s) 629, 1083
BsaAI YACGTR 1 cut(s) 731
BsaHI GRCGYC 2 cut(s) 594, 661
BsaJI CCNNGG 6 cut(s) 19, 170, 228, 600, 1032, 1111
BsaWI WCCGGW 1 cut(s) 971
BsaXI ACNNNNNCTCC 2 cut(s) 998, 1028
Bsc4I CCNNNNNNNGG 5 cut(s) 171, 271, 820, 957, 1216
Bse118I RCCGGY 1 cut(s) 971
Bse1I ACTGG 1 cut(s) 1160
Bse3DI GCAATG 1 cut(s) 340
BseBI CCWGG 4 cut(s) 20, 44, 530, 544
BseCI ATCGAT 2 cut(s) 629, 1083
BseDI CCNNGG 6 cut(s) 19, 170, 228, 600, 1032, 1111
BseGI GGATG 1 cut(s) 1195
BseLI CCNNNNNNNGG 5 cut(s) 171, 271, 820, 957, 1216
BseMI GCAATG 1 cut(s) 340
BseMII CTCAG 2 cut(s) 663, 1198
BseNI ACTGG 1 cut(s) 1160
BseRI GAGGAG 1 cut(s) 689
BseSI GKGCMC 2 cut(s) 1119, 1209
BseXI GCAGC 1 cut(s) 702
Bsh1236I CGCG 3 cut(s) 230, 267, 655
BshFI GGCC 5 cut(s) 275, 285, 608, 1031, 1037
BshNI GGYRCC 1 cut(s) 866
BshTI ACCGGT 1 cut(s) 971
BshVI ATCGAT 2 cut(s) 629, 1083
BsiHKAI GWGCWC 2 cut(s) 1142, 1209
BsiHKCI CYCGRG 2 cut(s) 111, 803
BsiSI CCGG 2 cut(s) 951, 972
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 5 cut(s) 171, 271, 820, 957, 1216
BsmAI GTCTC 4 cut(s) 166, 466, 789, 923
BsmBI CGTCTC 2 cut(s) 166, 466
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 1 cut(s) 1106
BsnI GGCC 5 cut(s) 275, 285, 608, 1031, 1037
BsoBI CYCGRG 2 cut(s) 111, 803
Bsp1286I GDGCHC 3 cut(s) 1119, 1142, 1209
Bsp1407I TGTACA 1 cut(s) 918
Bsp143I GATC 7 cut(s) 99, 156, 363, 385, 423, 939, 1080
BspACI CCGC 9 cut(s) 204, 228, 230, 265, 314, 477, 513, 540, 858
BspANI GGCC 5 cut(s) 275, 285, 608, 1031, 1037
BspCNI CTCAG 2 cut(s) 664, 1197
BspDI ATCGAT 2 cut(s) 629, 1083
BspFNI CGCG 3 cut(s) 230, 267, 655
BspLI GGNNCC 3 cut(s) 101, 234, 868
BspPI GGATC 4 cut(s) 94, 107, 358, 393
BspT107I GGYRCC 1 cut(s) 866
BsrDI GCAATG 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 971
BsrGI TGTACA 1 cut(s) 918
BsrI ACTGG 1 cut(s) 1160
BssAI RCCGGY 1 cut(s) 971
BssECI CCNNGG 6 cut(s) 19, 170, 228, 600, 1032, 1111
BssMI GATC 7 cut(s) 99, 156, 363, 385, 423, 939, 1080
BssNI GRCGYC 2 cut(s) 594, 661
BssT1I CCWWGG 1 cut(s) 1111
Bst2UI CCWGG 4 cut(s) 20, 44, 530, 544
Bst4CI ACNGT 1 cut(s) 601
Bst6I CTCTTC 1 cut(s) 1005
BstACI GRCGYC 2 cut(s) 594, 661
BstAUI TGTACA 1 cut(s) 918
BstBAI YACGTR 1 cut(s) 731
BstC8I GCNNGC 2 cut(s) 842, 1106
BstDEI CTNAG 3 cut(s) 88, 672, 1184
BstDSI CCRYGG 3 cut(s) 170, 228, 600
BstF5I GGATG 1 cut(s) 1195
BstFNI CGCG 3 cut(s) 230, 267, 655
BstHHI GCGC 2 cut(s) 526, 657
BstKTI GATC 7 cut(s) 102, 159, 366, 388, 426, 942, 1083
BstMAI GTCTC 4 cut(s) 166, 466, 789, 923
BstMBI GATC 7 cut(s) 99, 156, 363, 385, 423, 939, 1080
BstMWI GCNNNNNNNGC 5 cut(s) 53, 677, 750, 864, 1114
BstNI CCWGG 4 cut(s) 20, 44, 530, 544
BstNSI RCATGY 1 cut(s) 556
BstSCI CCNGG 4 cut(s) 18, 42, 528, 542
BstSLI GKGCMC 2 cut(s) 1119, 1209
BstUI CGCG 3 cut(s) 230, 267, 655
BstV1I GCAGC 1 cut(s) 702
BstV2I GAAGAC 2 cut(s) 448, 817
BstX2I RGATCY 3 cut(s) 99, 363, 385
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 3 cut(s) 99, 363, 385
Bsu15I ATCGAT 2 cut(s) 629, 1083
BsuI GTATCC 1 cut(s) 803
BsuRI GGCC 5 cut(s) 275, 285, 608, 1031, 1037
BsuTUI ATCGAT 2 cut(s) 629, 1083
BtgI CCRYGG 3 cut(s) 170, 228, 600
BtsCI GGATG 1 cut(s) 1195
BtsI GCAGTG 3 cut(s) 334, 1166, 1209
BtsIMutI CAGTG 3 cut(s) 334, 1166, 1209
Cac8I GCNNGC 2 cut(s) 842, 1106
CfoI GCGC 2 cut(s) 526, 657
Cfr10I RCCGGY 1 cut(s) 971
Cfr13I GGNCC 3 cut(s) 16, 232, 532
Cfr42I CCGCGG 1 cut(s) 231
ClaI ATCGAT 2 cut(s) 629, 1083
CseI GACGC 2 cut(s) 602, 669
Csp6I GTAC 3 cut(s) 85, 919, 1174
CspAI ACCGGT 1 cut(s) 971
CviAII CATG 5 cut(s) 145, 302, 481, 553, 610
CviQI GTAC 3 cut(s) 85, 919, 1174
DdeI CTNAG 3 cut(s) 88, 672, 1184
DpnI GATC 7 cut(s) 101, 158, 365, 387, 425, 941, 1082
DpnII GATC 7 cut(s) 99, 156, 363, 385, 423, 939, 1080
DrdI GACNNNNNNGTC 2 cut(s) 113, 976
DseDI GACNNNNNNGTC 2 cut(s) 113, 976
EaeI YGGCCR 2 cut(s) 273, 606
Eam1104I CTCTTC 1 cut(s) 1005
EarI CTCTTC 1 cut(s) 1005
EciI GGCGGA 2 cut(s) 466, 529
Eco130I CCWWGG 1 cut(s) 1111
Eco147I AGGCCT 1 cut(s) 285
Eco47I GGWCC 3 cut(s) 16, 232, 532
Eco57I CTGAAG 1 cut(s) 950
Eco72I CACGTG 1 cut(s) 731
Eco88I CYCGRG 2 cut(s) 111, 803
EcoO109I RGGNCCY 1 cut(s) 532
EcoRII CCWGG 4 cut(s) 18, 42, 528, 542
EcoT14I CCWWGG 1 cut(s) 1111
ErhI CCWWGG 1 cut(s) 1111
Esp3I CGTCTC 2 cut(s) 166, 466
FaeI CATG 5 cut(s) 148, 305, 484, 556, 613
FaiI YATR 8 cut(s) 146, 290, 303, 482, 554, 611, 977, 1017
FaqI GGGAC 1 cut(s) 455
FatI CATG 5 cut(s) 144, 301, 480, 552, 609
FauI CCCGC 1 cut(s) 235
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 2 cut(s) 514, 716
FokI GGATG 1 cut(s) 1202
Fsp4HI GCNGC 2 cut(s) 514, 716
GlaI GCGC 2 cut(s) 525, 656
GluI GCNGC 2 cut(s) 514, 716
GsuI CTGGAG 1 cut(s) 512
HaeIII GGCC 5 cut(s) 275, 285, 608, 1031, 1037
HapII CCGG 2 cut(s) 951, 972
HgaI GACGC 2 cut(s) 602, 669
HhaI GCGC 2 cut(s) 526, 657
Hin1I GRCGYC 2 cut(s) 594, 661
Hin1II CATG 5 cut(s) 148, 305, 484, 556, 613
Hin6I GCGC 2 cut(s) 524, 655
HinP1I GCGC 2 cut(s) 524, 655
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HinfI GANTC 9 cut(s) 66, 107, 114, 213, 499, 700, 797, 895, 947
HpaII CCGG 2 cut(s) 951, 972
HphI GGTGA 1 cut(s) 1081
Hpy166II GTNNAC 4 cut(s) 400, 442, 577, 1207
Hpy188I TCNGA 4 cut(s) 29, 65, 584, 969
Hpy188III TCNNGA 3 cut(s) 111, 154, 704
Hpy8I GTNNAC 4 cut(s) 400, 442, 577, 1207
Hpy99I CGWCG 6 cut(s) 407, 470, 596, 663, 836, 947
HpyAV CCTTC 9 cut(s) 275, 304, 460, 483, 567, 679, 848, 1021, 1187
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 2 cut(s) 730, 834
HpyCH4V TGCA 4 cut(s) 1060, 1104, 1202, 1207
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 677, 750, 864, 1114
HpyF3I CTNAG 3 cut(s) 88, 672, 1184
HpySE526I ACGT 2 cut(s) 730, 834
Hsp92I GRCGYC 2 cut(s) 594, 661
Hsp92II CATG 5 cut(s) 148, 305, 484, 556, 613
HspAI GCGC 2 cut(s) 524, 655
KspI CCGCGG 1 cut(s) 231
Kzo9I GATC 7 cut(s) 99, 156, 363, 385, 423, 939, 1080
LmnI GCTCC 1 cut(s) 531
Lsp1109I GCAGC 1 cut(s) 702
LweI GCATC 1 cut(s) 1047
MaeII ACGT 2 cut(s) 730, 834
MaeIII GTNAC 1 cut(s) 180
MalI GATC 7 cut(s) 101, 158, 365, 387, 425, 941, 1082
MboI GATC 7 cut(s) 99, 156, 363, 385, 423, 939, 1080
MflI RGATCY 3 cut(s) 99, 363, 385
MhlI GDGCHC 3 cut(s) 1119, 1142, 1209
MlsI TGGCCA 2 cut(s) 275, 608
MluCI AATT 3 cut(s) 10, 907, 1132
MluNI TGGCCA 2 cut(s) 275, 608
MlyI GAGTC 6 cut(s) 101, 123, 493, 791, 889, 956
MmeI TCCRAC 1 cut(s) 969
Mox20I TGGCCA 2 cut(s) 275, 608
MroXI GAANNNNTTC 1 cut(s) 348
MscI TGGCCA 2 cut(s) 275, 608
MseI TTAA 2 cut(s) 1002, 1222
MslI CAYNNNNRTG 2 cut(s) 395, 602
Msp20I TGGCCA 2 cut(s) 275, 608
MspA1I CMGCKG 2 cut(s) 230, 873
MspI CCGG 2 cut(s) 951, 972
MspR9I CCNGG 4 cut(s) 20, 44, 530, 544
Mva1269I GAATGC 1 cut(s) 1106
MvaI CCWGG 4 cut(s) 20, 44, 530, 544
MvnI CGCG 3 cut(s) 230, 267, 655
MwoI GCNNNNNNNGC 5 cut(s) 53, 677, 750, 864, 1114
NdeII GATC 7 cut(s) 99, 156, 363, 385, 423, 939, 1080
NlaIII CATG 5 cut(s) 148, 305, 484, 556, 613
NlaIV GGNNCC 3 cut(s) 101, 234, 868
NmeAIII GCCGAG 1 cut(s) 1057
NmuCI GTSAC 1 cut(s) 180
NspI RCATGY 1 cut(s) 556
OliI CACNNNNGTG 1 cut(s) 602
PaeR7I CTCGAG 1 cut(s) 803
PceI AGGCCT 1 cut(s) 285
PcsI WCGNNNNNNNCGW 3 cut(s) 465, 597, 746
PctI GAATGC 1 cut(s) 1106
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 3 cut(s) 66, 213, 700
PfoI TCCNGGA 1 cut(s) 528
PinAI ACCGGT 1 cut(s) 971
PkrI GCNGC 2 cut(s) 515, 717
PleI GAGTC 6 cut(s) 101, 122, 493, 791, 889, 955
PmaCI CACGTG 1 cut(s) 731
PmlI CACGTG 1 cut(s) 731
PpsI GAGTC 6 cut(s) 101, 122, 493, 791, 889, 955
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 1 cut(s) 532
Psp5II RGGWCCY 1 cut(s) 532
Psp6I CCWGG 4 cut(s) 18, 42, 528, 542
PspCI CACGTG 1 cut(s) 731
PspGI CCWGG 4 cut(s) 18, 42, 528, 542
PspN4I GGNNCC 3 cut(s) 101, 234, 868
PspPI GGNCC 3 cut(s) 16, 232, 532
PspPPI RGGWCCY 1 cut(s) 532
PspXI VCTCGAGB 1 cut(s) 803
PsuI RGATCY 3 cut(s) 99, 363, 385
PvuII CAGCTG 1 cut(s) 873
RsaI GTAC 3 cut(s) 86, 920, 1175
RsaNI GTAC 3 cut(s) 85, 919, 1174
RseI CAYNNNNRTG 2 cut(s) 395, 602
SacII CCGCGG 1 cut(s) 231
SaqAI TTAA 2 cut(s) 1002, 1222
SatI GCNGC 2 cut(s) 514, 716
Sau3AI GATC 7 cut(s) 99, 156, 363, 385, 423, 939, 1080
Sau96I GGNCC 3 cut(s) 16, 232, 532
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 6 cut(s) 101, 123, 493, 791, 889, 956
ScrFI CCNGG 4 cut(s) 20, 44, 530, 544
SduI GDGCHC 3 cut(s) 1119, 1142, 1209
SfaNI GCATC 1 cut(s) 1047
Sfr274I CTCGAG 1 cut(s) 803
Sfr303I CCGCGG 1 cut(s) 231
SgrBI CCGCGG 1 cut(s) 231
SinI GGWCC 3 cut(s) 16, 232, 532
SlaI CTCGAG 1 cut(s) 803
SmiMI CAYNNNNRTG 2 cut(s) 395, 602
SmlI CTYRAG 2 cut(s) 503, 803
SmoI CTYRAG 2 cut(s) 503, 803
Sse9I AATT 3 cut(s) 10, 907, 1132
SseBI AGGCCT 1 cut(s) 285
SsiI CCGC 9 cut(s) 204, 228, 230, 265, 314, 477, 513, 540, 858
StuI AGGCCT 1 cut(s) 285
StyD4I CCNGG 4 cut(s) 18, 42, 528, 542
StyI CCWWGG 1 cut(s) 1111
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 2 cut(s) 733, 837
TasI AATT 3 cut(s) 10, 907, 1132
TatI WGTACW 2 cut(s) 84, 918
TauI GCSGC 1 cut(s) 516
TfiI GAWTC 3 cut(s) 66, 213, 700
Tru1I TTAA 2 cut(s) 1002, 1222
Tru9I TTAA 2 cut(s) 1002, 1222
TscAI CASTG 3 cut(s) 334, 1166, 1209
TseFI GTSAC 1 cut(s) 180
TseI GCWGC 1 cut(s) 715
Tsp45I GTSAC 1 cut(s) 180
TspDTI ATGAA 9 cut(s) 354, 478, 497, 615, 726, 817, 1040, 1088, 1206
TspGWI ACGGA 5 cut(s) 154, 159, 385, 454, 1191
TspRI CASTG 3 cut(s) 334, 1166, 1209
VneI GTGCAC 1 cut(s) 1205
VpaK11BI GGWCC 3 cut(s) 16, 232, 532
XapI RAATTY 1 cut(s) 907
XceI RCATGY 1 cut(s) 556
XcmI CCANNNNNNNNNTGG 2 cut(s) 40, 1118
XhoI CTCGAG 1 cut(s) 803
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 1 cut(s) 348
ZrmI AGTACT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.