MD09G1227400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011711
Physical Location & Seq
Reverse (-)
28092569 .. 28093087
519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1227400.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGGGAAGACCAGCACCCACGCAACCGAAACTTCATCCTCTACCACCACCTCTCCCACAAGAAGAAGGCTATTGGCTGAGGAGGCAAAGAATGAGGCGTTGAAGAGGCTAGAAGATGTGCTTCTAAAACCCCCTAGAGCCTCGTCTGGGAAATCGAGGTGGTACTTGAAGCGAACGAGGTTCCTCCCAGACTTGATGGGTGACTCGCTCGAAAGCTATCCTAGAAGGGTTTTTATTGACGTAGGATTACTGGAGAATGAAGGCGGAAGTGGGACGAGCTGGTTTGCGAAGAACTACCCTACCCGGAACAAGAACTTTGAGATGTACAAGATCAAGACTGTGATAGAGGAGAGTTCCGGGAAGGAGGTAGTTCAGACTAGAATGTCAGAGTGGCTGAACAAGAATGTTAAGGAGAAGGAGTACGTGGTGATGAAGGCCGAGGCCGAGGTGGTGGATGAGATGGTGAAAAGTAGAGCGATTCACTTGGTGGATGAGCTTTTCTTGGAGTGCAAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.74

Weight (kDa)

9.1

Isoelectric Point (pI)

54.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 34 - 171 6.4e-60 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 382
AciI CCGC 1 cut(s) 264
AdeI CACNNNGTG 1 cut(s) 487
AfaI GTAC 3 cut(s) 164, 326, 422
AfiI CCNNNNNNNGG 1 cut(s) 147
AgsI TTSAA 2 cut(s) 103, 169
AluBI AGCT 3 cut(s) 216, 279, 496
AluI AGCT 3 cut(s) 216, 279, 496
AoxI GGCC 2 cut(s) 435, 441
AsuC2I CCSGG 2 cut(s) 304, 358
AsuHPI GGTGA 3 cut(s) 212, 439, 475
BbsI GAAGAC 1 cut(s) 14
BbvCI CCTCAGC 1 cut(s) 78
BccI CCATC 2 cut(s) 190, 454
BcnI CCSGG 2 cut(s) 304, 358
BfaI CTAG 4 cut(s) 110, 135, 222, 378
Bme1390I CCNGG 2 cut(s) 304, 358
BmiI GGNNCC 1 cut(s) 182
BmrFI CCNGG 2 cut(s) 304, 358
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 272
Bpu10I CCTNAGC 1 cut(s) 78
BpuMI CCSGG 2 cut(s) 304, 358
BsaAI YACGTR 1 cut(s) 424
BsaJI CCNNGG 2 cut(s) 438, 444
BsaXI ACNNNNNCTCC 4 cut(s) 37, 67, 404, 434
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 1 cut(s) 255
BseDI CCNNGG 2 cut(s) 438, 444
BseGI GGATG 3 cut(s) 35, 460, 496
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 255
BseRI GAGGAG 2 cut(s) 95, 362
BshFI GGCC 2 cut(s) 437, 443
BsiSI CCGG 2 cut(s) 304, 357
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 286
BsnI GGCC 2 cut(s) 437, 443
Bsp1407I TGTACA 1 cut(s) 324
Bsp143I GATC 1 cut(s) 330
BspACI CCGC 1 cut(s) 264
BspANI GGCC 2 cut(s) 437, 443
BspCNI CTCAG 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 324
BsrI ACTGG 1 cut(s) 255
BssECI CCNNGG 2 cut(s) 438, 444
BssMI GATC 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 340
Bst6I CTCTTC 1 cut(s) 98
BstAUI TGTACA 1 cut(s) 324
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 1 cut(s) 512
BstDEI CTNAG 1 cut(s) 78
BstF5I GGATG 3 cut(s) 35, 460, 496
BstKTI GATC 1 cut(s) 333
BstMBI GATC 1 cut(s) 330
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstSCI CCNGG 2 cut(s) 302, 356
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 2 cut(s) 437, 443
BtsCI GGATG 3 cut(s) 35, 460, 496
Cac8I GCNNGC 1 cut(s) 512
Csp6I GTAC 3 cut(s) 163, 325, 421
CviQI GTAC 3 cut(s) 163, 325, 421
DdeI CTNAG 1 cut(s) 78
DpnI GATC 1 cut(s) 332
DpnII GATC 1 cut(s) 330
DraIII CACNNNGTG 1 cut(s) 487
DrdI GACNNNNNNGTC 1 cut(s) 382
DseDI GACNNNNNNGTC 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
EciI GGCGGA 1 cut(s) 279
FaiI YATR 1 cut(s) 517
FalI AAGNNNNNCTT 2 cut(s) 105, 137
FaqI GGGAC 1 cut(s) 286
FokI GGATG 3 cut(s) 22, 467, 503
FspBI CTAG 4 cut(s) 110, 135, 222, 378
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 2 cut(s) 437, 443
HapII CCGG 2 cut(s) 304, 357
HinfI GANTC 2 cut(s) 203, 478
HpaII CCGG 2 cut(s) 304, 357
HphI GGTGA 3 cut(s) 212, 439, 475
Hpy188I TCNGA 2 cut(s) 375, 388
Hpy188III TCNNGA 1 cut(s) 334
HpyAV CCTTC 6 cut(s) 60, 219, 254, 355, 409, 427
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4IV ACGT 2 cut(s) 240, 423
HpyCH4V TGCA 1 cut(s) 510
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 1 cut(s) 78
HpySE526I ACGT 2 cut(s) 240, 423
Kzo9I GATC 1 cut(s) 330
LpnPI CCDG 7 cut(s) 25, 132, 201, 236, 265, 317, 370
MaeI CTAG 4 cut(s) 110, 135, 222, 378
MaeII ACGT 2 cut(s) 240, 423
MaeIII GTNAC 1 cut(s) 200
MalI GATC 1 cut(s) 332
MboI GATC 1 cut(s) 330
MboII GAAGA 5 cut(s) 19, 75, 115, 125, 301
MlyI GAGTC 1 cut(s) 197
MseI TTAA 1 cut(s) 408
MspI CCGG 2 cut(s) 304, 357
MspR9I CCNGG 2 cut(s) 304, 358
MwoI GCNNNNNNNGC 1 cut(s) 83
NciI CCSGG 2 cut(s) 304, 358
NdeII GATC 1 cut(s) 330
NlaIV GGNNCC 1 cut(s) 182
NmeAIII GCCGAG 2 cut(s) 463, 469
NmuCI GTSAC 1 cut(s) 200
PfeI GAWTC 1 cut(s) 478
PfoI TCCNGGA 1 cut(s) 356
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Ppu21I YACGTR 1 cut(s) 424
PspN4I GGNNCC 1 cut(s) 182
RsaI GTAC 3 cut(s) 164, 326, 422
RsaNI GTAC 3 cut(s) 163, 325, 421
SaqAI TTAA 1 cut(s) 408
Sau3AI GATC 1 cut(s) 330
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 2 cut(s) 304, 358
SsiI CCGC 1 cut(s) 264
SspMI CTAG 4 cut(s) 110, 135, 222, 378
StyD4I CCNGG 2 cut(s) 302, 356
TaaI ACNGT 1 cut(s) 340
TaiI ACGT 2 cut(s) 243, 426
TaqI TCGA 2 cut(s) 155, 210
TatI WGTACW 1 cut(s) 324
TfiI GAWTC 1 cut(s) 478
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TseFI GTSAC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 3 cut(s) 24, 273, 446
XspI CTAG 4 cut(s) 110, 135, 222, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.