Rh7DG444000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0011711
Physical Location & Seq
Reverse (-)
63011493 .. 63013681
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG444000.1

Sequence Viewer

Length: 1218 bp
ATGGCGATCAATTTGGTCCAGGGCTTCCGAACCAAACAACACCTGGCAGACAAAGCCACCATCGGATTCTACCCCGATGTCAAGTACTTAGTTTTTAGGATCCCTGACTCCCGAGTCTTGTTGTTTCTGGCTCGATGTTCCATCTTGGCTTTCGTGATCGTCTCCGTTCCGTGGTTGAAGTCACTTGTTCCCACAACAGCCACCCCTAATGGAATCATCGTCTTCCCCGCGGACCCTAATATCGAGCTTGTGCCACTCATCTTCCGCGACTTGGCCAACGAAGGCCTCATCAAAACCCGTCATGGCGAGAAGGCAGTGTTTGTCAGCAGTGGCAATGATGAAGAAGCCATTCAAAGCTCACAGATCCTCAAAGACAACGAAATGGATCTCATTTCCGTGAACGACGAAAACCAACAGAACTCGATCAACGACCAATCTGTTGACTTCGCCTTCGTCTCCGTCGTCCCTTCCTCCATGAAGTTCATCGAAAGGACTCTCAAGGTCGGCGGCATTGTTGCGCTCCAGGACCTCTCCGCCAGGTTTCACATGCCTTCTAACTACAAGATGGTCTACCTCCGAAAACTCGACGCCACCGTGGTGGCCATGAGGAAGATGAGTTCATCGATTTCAACTACTACTTCTTTCACGCGCCGACGCCTATTTGCTGAGGAGGCAAAGAAGGAAGCATTGAAGAATCTCGAAGATGTGCTGCTCGAACCCCCAAGTGCTTCATCGGGCAAGTCAAGCCGATACTTGAAGAAAACTCGCTACCTACCCGACTTGATGGGAGACTCGCTCGAGGGATACCCTCGCAGGGTCTTCATCGACGTTGGCTTGCCTGAAAAGGAAGGCGGTAGTGGCACCAGCTGGTTCGCCAAGAACTACCCAACTCGCAACAAGAATTTTGAGATGTACAAGATTGAGACAACCCTGATCGACGAGTCCGGCAAGGGGATTCTTCAGACCGGTATGTCGGGGTGGTTGGAGAAGAATGTGAAGGAGGAAGAGTATGTTGTGATGAAGGCCGAGGCCGAAGTGGTGGAGGAAATGATGCAGGGTAAGGTGATGAACTTGGTCGATGAGCTTTTCTTGGAATGCAAGCCACAAGGGCACAATGGGAAAGGTAATTTGAGCACTCGTAGGGCTTACTGGCAGTGCTTGGCTTTGTACGGACAGCTCAGGGATGAAGGTGTTGCAGTGCACCAATGGTGGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

405

Amino Acids

45.6

Weight (kDa)

6.99

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 230 - 404 9.9e-81 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 113, 970
AccB1I GGYRCC 1 cut(s) 860
AccI GTMKAC 1 cut(s) 570
AccII CGCG 3 cut(s) 230, 267, 649
AciI CCGC 6 cut(s) 228, 230, 265, 507, 534, 852
AclWI GGATC 4 cut(s) 94, 107, 358, 393
AcoI YGGCCR 2 cut(s) 273, 600
AcsI RAATTY 1 cut(s) 901
AcuI CTGAAG 1 cut(s) 944
AcyI GRCGYC 2 cut(s) 588, 655
AfaI GTAC 3 cut(s) 86, 914, 1169
AfiI CCNNNNNNNGG 5 cut(s) 171, 271, 814, 951, 1210
AgeI ACCGGT 1 cut(s) 965
AgsI TTSAA 5 cut(s) 178, 353, 630, 691, 757
AjnI CCWGG 4 cut(s) 18, 42, 522, 536
AleI CACNNNNGTG 1 cut(s) 596
AloI GAACNNNNNNTCC 2 cut(s) 601, 633
AluBI AGCT 5 cut(s) 247, 357, 867, 1084, 1177
AluI AGCT 5 cut(s) 247, 357, 867, 1084, 1177
Alw21I GWGCWC 2 cut(s) 1136, 1203
Alw26I GTCTC 4 cut(s) 166, 460, 783, 917
Alw44I GTGCAC 1 cut(s) 1199
AlwI GGATC 4 cut(s) 94, 107, 358, 393
Ama87I CYCGRG 2 cut(s) 111, 797
AoxI GGCC 5 cut(s) 273, 283, 600, 1023, 1029
ApaLI GTGCAC 1 cut(s) 1199
ApeKI GCWGC 1 cut(s) 709
ApoI RAATTY 1 cut(s) 901
ArsI GACNNNNNNTTYG 2 cut(s) 434, 466
AsiGI ACCGGT 1 cut(s) 965
Asp700I GAANNNNTTC 1 cut(s) 348
AspLEI GCGC 2 cut(s) 520, 651
AspS9I GGNCC 3 cut(s) 16, 232, 526
AsuHPI GGTGA 1 cut(s) 1075
AvaI CYCGRG 2 cut(s) 111, 797
AvaII GGWCC 3 cut(s) 16, 232, 526
BaeGI GKGCMC 2 cut(s) 1113, 1203
BalI TGGCCA 2 cut(s) 275, 602
BamHI GGATCC 1 cut(s) 99
BanI GGYRCC 1 cut(s) 860
BbsI GAAGAC 2 cut(s) 214, 811
Bbv12I GWGCWC 2 cut(s) 1136, 1203
BbvCI CCTCAGC 1 cut(s) 666
BbvI GCAGC 1 cut(s) 696
BccI CCATC 4 cut(s) 68, 149, 559, 778
BciT130I CCWGG 4 cut(s) 20, 44, 524, 538
BciVI GTATCC 1 cut(s) 797
BcoDI GTCTC 4 cut(s) 166, 460, 783, 917
BfuI GTATCC 1 cut(s) 797
BglI GCCNNNNNGGC 1 cut(s) 1108
BisI GCNGC 2 cut(s) 508, 710
BlsI GCNGC 2 cut(s) 509, 711
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 4 cut(s) 20, 44, 524, 538
Bme18I GGWCC 3 cut(s) 16, 232, 526
BmeT110I CYCGRG 2 cut(s) 111, 797
BmgT120I GGNCC 3 cut(s) 16, 232, 526
BmiI GGNNCC 3 cut(s) 101, 234, 862
BmrFI CCNGG 4 cut(s) 20, 44, 524, 538
BmsI GCATC 1 cut(s) 1041
BpiI GAAGAC 2 cut(s) 214, 811
BplI GAGNNNNNCTC 2 cut(s) 780, 812
BpmI CTGGAG 1 cut(s) 506
Bpu10I CCTNAGC 2 cut(s) 666, 1178
BpuEI CTTGAG 1 cut(s) 482
Bsa29I ATCGAT 1 cut(s) 623
BsaHI GRCGYC 2 cut(s) 588, 655
BsaJI CCNNGG 5 cut(s) 19, 170, 228, 594, 1026
BsaWI WCCGGW 1 cut(s) 965
BsaXI ACNNNNNCTCC 2 cut(s) 992, 1022
Bsc4I CCNNNNNNNGG 5 cut(s) 171, 271, 814, 951, 1210
Bse118I RCCGGY 1 cut(s) 965
Bse1I ACTGG 1 cut(s) 1154
Bse3DI GCAATG 1 cut(s) 340
BseBI CCWGG 4 cut(s) 20, 44, 524, 538
BseCI ATCGAT 1 cut(s) 623
BseDI CCNNGG 5 cut(s) 19, 170, 228, 594, 1026
BseGI GGATG 1 cut(s) 1189
BseLI CCNNNNNNNGG 5 cut(s) 171, 271, 814, 951, 1210
BseMI GCAATG 1 cut(s) 340
BseMII CTCAG 2 cut(s) 657, 1192
BseNI ACTGG 1 cut(s) 1154
BseRI GAGGAG 1 cut(s) 683
BseSI GKGCMC 2 cut(s) 1113, 1203
BseXI GCAGC 1 cut(s) 696
Bsh1236I CGCG 3 cut(s) 230, 267, 649
BshFI GGCC 5 cut(s) 275, 285, 602, 1025, 1031
BshNI GGYRCC 1 cut(s) 860
BshTI ACCGGT 1 cut(s) 965
BshVI ATCGAT 1 cut(s) 623
BsiHKAI GWGCWC 2 cut(s) 1136, 1203
BsiHKCI CYCGRG 2 cut(s) 111, 797
BsiSI CCGG 2 cut(s) 945, 966
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 5 cut(s) 171, 271, 814, 951, 1210
BsmAI GTCTC 4 cut(s) 166, 460, 783, 917
BsmBI CGTCTC 2 cut(s) 166, 460
BsmFI GGGAC 1 cut(s) 449
BsmI GAATGC 1 cut(s) 1100
BsnI GGCC 5 cut(s) 275, 285, 602, 1025, 1031
BsoBI CYCGRG 2 cut(s) 111, 797
Bsp1286I GDGCHC 3 cut(s) 1113, 1136, 1203
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 7 cut(s) 6, 99, 156, 363, 385, 423, 933
BspACI CCGC 6 cut(s) 228, 230, 265, 507, 534, 852
BspANI GGCC 5 cut(s) 275, 285, 602, 1025, 1031
BspCNI CTCAG 2 cut(s) 658, 1191
BspDI ATCGAT 1 cut(s) 623
BspFNI CGCG 3 cut(s) 230, 267, 649
BspLI GGNNCC 3 cut(s) 101, 234, 862
BspPI GGATC 4 cut(s) 94, 107, 358, 393
BspT107I GGYRCC 1 cut(s) 860
BsrDI GCAATG 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 965
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 1 cut(s) 1154
BssAI RCCGGY 1 cut(s) 965
BssECI CCNNGG 5 cut(s) 19, 170, 228, 594, 1026
BssMI GATC 7 cut(s) 6, 99, 156, 363, 385, 423, 933
BssNI GRCGYC 2 cut(s) 588, 655
Bst2UI CCWGG 4 cut(s) 20, 44, 524, 538
Bst4CI ACNGT 1 cut(s) 595
Bst6I CTCTTC 1 cut(s) 999
BstACI GRCGYC 2 cut(s) 588, 655
BstAUI TGTACA 1 cut(s) 912
BstC8I GCNNGC 2 cut(s) 836, 1100
BstDEI CTNAG 3 cut(s) 88, 666, 1178
BstDSI CCRYGG 3 cut(s) 170, 228, 594
BstF5I GGATG 1 cut(s) 1189
BstFNI CGCG 3 cut(s) 230, 267, 649
BstHHI GCGC 2 cut(s) 520, 651
BstKTI GATC 7 cut(s) 9, 102, 159, 366, 388, 426, 936
BstMAI GTCTC 4 cut(s) 166, 460, 783, 917
BstMBI GATC 7 cut(s) 6, 99, 156, 363, 385, 423, 933
BstMWI GCNNNNNNNGC 5 cut(s) 53, 671, 744, 858, 1108
BstNI CCWGG 4 cut(s) 20, 44, 524, 538
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 4 cut(s) 18, 42, 522, 536
BstSLI GKGCMC 2 cut(s) 1113, 1203
BstUI CGCG 3 cut(s) 230, 267, 649
BstV1I GCAGC 1 cut(s) 696
BstV2I GAAGAC 2 cut(s) 214, 811
BstX2I RGATCY 3 cut(s) 99, 363, 385
BstXI CCANNNNNNTGG 1 cut(s) 598
BstYI RGATCY 3 cut(s) 99, 363, 385
Bsu15I ATCGAT 1 cut(s) 623
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 5 cut(s) 275, 285, 602, 1025, 1031
BsuTUI ATCGAT 1 cut(s) 623
BtgI CCRYGG 3 cut(s) 170, 228, 594
BtsCI GGATG 1 cut(s) 1189
BtsI GCAGTG 4 cut(s) 321, 334, 1160, 1203
BtsIMutI CAGTG 4 cut(s) 321, 334, 1160, 1203
Cac8I GCNNGC 2 cut(s) 836, 1100
CfoI GCGC 2 cut(s) 520, 651
Cfr10I RCCGGY 1 cut(s) 965
Cfr13I GGNCC 3 cut(s) 16, 232, 526
Cfr42I CCGCGG 1 cut(s) 231
ClaI ATCGAT 1 cut(s) 623
CseI GACGC 2 cut(s) 596, 663
Csp6I GTAC 3 cut(s) 85, 913, 1168
CspAI ACCGGT 1 cut(s) 965
CviAII CATG 4 cut(s) 302, 475, 547, 604
CviQI GTAC 3 cut(s) 85, 913, 1168
DdeI CTNAG 3 cut(s) 88, 666, 1178
DpnI GATC 7 cut(s) 8, 101, 158, 365, 387, 425, 935
DpnII GATC 7 cut(s) 6, 99, 156, 363, 385, 423, 933
DrdI GACNNNNNNGTC 2 cut(s) 113, 970
DseDI GACNNNNNNGTC 2 cut(s) 113, 970
EaeI YGGCCR 2 cut(s) 273, 600
Eam1104I CTCTTC 1 cut(s) 999
EarI CTCTTC 1 cut(s) 999
EciI GGCGGA 1 cut(s) 523
Eco147I AGGCCT 1 cut(s) 285
Eco47I GGWCC 3 cut(s) 16, 232, 526
Eco57I CTGAAG 1 cut(s) 944
Eco88I CYCGRG 2 cut(s) 111, 797
EcoO109I RGGNCCY 1 cut(s) 526
EcoRII CCWGG 4 cut(s) 18, 42, 522, 536
Esp3I CGTCTC 2 cut(s) 166, 460
FaeI CATG 4 cut(s) 305, 478, 550, 607
FaiI YATR 6 cut(s) 303, 476, 548, 605, 971, 1011
FaqI GGGAC 1 cut(s) 449
FatI CATG 4 cut(s) 301, 474, 546, 603
FauI CCCGC 1 cut(s) 235
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 2 cut(s) 508, 710
FokI GGATG 1 cut(s) 1196
Fsp4HI GCNGC 2 cut(s) 508, 710
GlaI GCGC 2 cut(s) 519, 650
GluI GCNGC 2 cut(s) 508, 710
GsuI CTGGAG 1 cut(s) 506
HaeIII GGCC 5 cut(s) 275, 285, 602, 1025, 1031
HapII CCGG 2 cut(s) 945, 966
HgaI GACGC 2 cut(s) 596, 663
HhaI GCGC 2 cut(s) 520, 651
Hin1I GRCGYC 2 cut(s) 588, 655
Hin1II CATG 4 cut(s) 305, 478, 550, 607
Hin6I GCGC 2 cut(s) 518, 649
HinP1I GCGC 2 cut(s) 518, 649
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HinfI GANTC 9 cut(s) 66, 107, 114, 213, 493, 694, 791, 941, 955
HpaII CCGG 2 cut(s) 945, 966
HphI GGTGA 1 cut(s) 1075
Hpy166II GTNNAC 4 cut(s) 400, 442, 571, 1201
Hpy188I TCNGA 4 cut(s) 29, 65, 578, 963
Hpy188III TCNNGA 3 cut(s) 111, 154, 698
Hpy8I GTNNAC 4 cut(s) 400, 442, 571, 1201
Hpy99I CGWCG 6 cut(s) 407, 464, 590, 657, 830, 941
HpyCH4III ACNGT 1 cut(s) 595
HpyCH4IV ACGT 1 cut(s) 828
HpyCH4V TGCA 4 cut(s) 1054, 1098, 1196, 1201
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 671, 744, 858, 1108
HpyF3I CTNAG 3 cut(s) 88, 666, 1178
HpySE526I ACGT 1 cut(s) 828
Hsp92I GRCGYC 2 cut(s) 588, 655
Hsp92II CATG 4 cut(s) 305, 478, 550, 607
HspAI GCGC 2 cut(s) 518, 649
KspI CCGCGG 1 cut(s) 231
Kzo9I GATC 7 cut(s) 6, 99, 156, 363, 385, 423, 933
LmnI GCTCC 1 cut(s) 525
Lsp1109I GCAGC 1 cut(s) 696
LweI GCATC 1 cut(s) 1041
MaeII ACGT 1 cut(s) 828
MaeIII GTNAC 1 cut(s) 180
MalI GATC 7 cut(s) 8, 101, 158, 365, 387, 425, 935
MboI GATC 7 cut(s) 6, 99, 156, 363, 385, 423, 933
MflI RGATCY 3 cut(s) 99, 363, 385
MhlI GDGCHC 3 cut(s) 1113, 1136, 1203
MlsI TGGCCA 2 cut(s) 275, 602
MluCI AATT 3 cut(s) 10, 901, 1126
MluNI TGGCCA 2 cut(s) 275, 602
MlyI GAGTC 5 cut(s) 101, 123, 487, 785, 950
MmeI TCCRAC 1 cut(s) 963
Mox20I TGGCCA 2 cut(s) 275, 602
MroXI GAANNNNTTC 1 cut(s) 348
MscI TGGCCA 2 cut(s) 275, 602
MseI TTAA 1 cut(s) 1216
MslI CAYNNNNRTG 2 cut(s) 395, 596
Msp20I TGGCCA 2 cut(s) 275, 602
MspA1I CMGCKG 2 cut(s) 230, 867
MspI CCGG 2 cut(s) 945, 966
MspR9I CCNGG 4 cut(s) 20, 44, 524, 538
Mva1269I GAATGC 1 cut(s) 1100
MvaI CCWGG 4 cut(s) 20, 44, 524, 538
MvnI CGCG 3 cut(s) 230, 267, 649
MwoI GCNNNNNNNGC 5 cut(s) 53, 671, 744, 858, 1108
NdeII GATC 7 cut(s) 6, 99, 156, 363, 385, 423, 933
NlaIII CATG 4 cut(s) 305, 478, 550, 607
NlaIV GGNNCC 3 cut(s) 101, 234, 862
NmeAIII GCCGAG 1 cut(s) 1051
NmuCI GTSAC 1 cut(s) 180
NspI RCATGY 1 cut(s) 550
OliI CACNNNNGTG 1 cut(s) 596
PaeR7I CTCGAG 1 cut(s) 797
PceI AGGCCT 1 cut(s) 285
PcsI WCGNNNNNNNCGW 2 cut(s) 459, 591
PctI GAATGC 1 cut(s) 1100
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 4 cut(s) 66, 213, 694, 955
PfoI TCCNGGA 1 cut(s) 522
PinAI ACCGGT 1 cut(s) 965
PkrI GCNGC 2 cut(s) 509, 711
PleI GAGTC 5 cut(s) 101, 122, 487, 785, 949
PpsI GAGTC 5 cut(s) 101, 122, 487, 785, 949
PpuMI RGGWCCY 1 cut(s) 526
Psp5II RGGWCCY 1 cut(s) 526
Psp6I CCWGG 4 cut(s) 18, 42, 522, 536
PspGI CCWGG 4 cut(s) 18, 42, 522, 536
PspN4I GGNNCC 3 cut(s) 101, 234, 862
PspPI GGNCC 3 cut(s) 16, 232, 526
PspPPI RGGWCCY 1 cut(s) 526
PspXI VCTCGAGB 1 cut(s) 797
PsuI RGATCY 3 cut(s) 99, 363, 385
PvuII CAGCTG 1 cut(s) 867
RsaI GTAC 3 cut(s) 86, 914, 1169
RsaNI GTAC 3 cut(s) 85, 913, 1168
RseI CAYNNNNRTG 2 cut(s) 395, 596
SacII CCGCGG 1 cut(s) 231
SaqAI TTAA 1 cut(s) 1216
SatI GCNGC 2 cut(s) 508, 710
Sau3AI GATC 7 cut(s) 6, 99, 156, 363, 385, 423, 933
Sau96I GGNCC 3 cut(s) 16, 232, 526
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 5 cut(s) 101, 123, 487, 785, 950
ScrFI CCNGG 4 cut(s) 20, 44, 524, 538
SduI GDGCHC 3 cut(s) 1113, 1136, 1203
SfaNI GCATC 1 cut(s) 1041
Sfr274I CTCGAG 1 cut(s) 797
Sfr303I CCGCGG 1 cut(s) 231
SgrBI CCGCGG 1 cut(s) 231
SinI GGWCC 3 cut(s) 16, 232, 526
SlaI CTCGAG 1 cut(s) 797
SmiMI CAYNNNNRTG 2 cut(s) 395, 596
SmlI CTYRAG 2 cut(s) 497, 797
SmoI CTYRAG 2 cut(s) 497, 797
Sse9I AATT 3 cut(s) 10, 901, 1126
SseBI AGGCCT 1 cut(s) 285
SsiI CCGC 6 cut(s) 228, 230, 265, 507, 534, 852
StuI AGGCCT 1 cut(s) 285
StyD4I CCNGG 4 cut(s) 18, 42, 522, 536
TaaI ACNGT 1 cut(s) 595
TaiI ACGT 1 cut(s) 831
TasI AATT 3 cut(s) 10, 901, 1126
TatI WGTACW 2 cut(s) 84, 912
TauI GCSGC 1 cut(s) 510
TfiI GAWTC 4 cut(s) 66, 213, 694, 955
Tru1I TTAA 1 cut(s) 1216
Tru9I TTAA 1 cut(s) 1216
TscAI CASTG 4 cut(s) 321, 334, 1160, 1203
TseFI GTSAC 1 cut(s) 180
TseI GCWGC 1 cut(s) 709
Tsp45I GTSAC 1 cut(s) 180
TspDTI ATGAA 9 cut(s) 354, 472, 491, 609, 720, 811, 1034, 1082, 1200
TspGWI ACGGA 5 cut(s) 154, 159, 385, 448, 1185
TspRI CASTG 4 cut(s) 321, 334, 1160, 1203
VneI GTGCAC 1 cut(s) 1199
VpaK11BI GGWCC 3 cut(s) 16, 232, 526
XapI RAATTY 1 cut(s) 901
XceI RCATGY 1 cut(s) 550
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XhoI CTCGAG 1 cut(s) 797
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 348
ZrmI AGTACT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.