MD15G1383600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011711
Physical Location & Seq
Forward (+)
47248714 .. 47249952
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1383600.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGCGATCAATTTGGTCCAAGGTTTCCGTACCAAACGCCACCCGAAGTATGCTGCTCCAATCGGATTGTATCCAGATGCCAAGTACTTGGTTATTCGAATTCCGGATTCTAGAGCCTTGATCCTCGTCGCTCGTTCTTTAATCTTGGCTTTGTGCATCGTCTTGCTCCCGCGGTTGAACTCACTCCTTCACACAGCAACCACTTCTTTGCCTGAAATAATTAGCTCATCCGACGTCGTTAATCTGAAGCTCCTTCCTTTGATCTTCCGTGACTTGGCCAACGAAGGCCTATACAAGAAAGACACACATAAAGCTATTTTCGTAAGCAGCGGAGACAAAGAAGCTGTCAAAGCATCTCAGTTCCTCAATAACGAATATATTAATGAGATCGAGCTTATCAACTCCGACATGCAAAGCTCAGTCCCTAAAAACTCAATCGACTTTGCATTCGTCTCTGCCTTCCCTGCCGCCTCAAAGTTCATTGAAAGGACTCTTAAAATTGGTGGGATTGTCGTGCTTCAGCTCAGCAATGACGCTTCCAACATCTTCAAGAAGCCTGACAACTATAAGATGGTATACCTGAGGCGATTTGACAAAACCATTTTTGCAATGAGGAAGACCAGCACCCGTGCAAGCAAAACTTCGTCCTCTACCACCACCTCTCCCACAAGGAGAAGGCTCTTGGCTAGGGAGGCAAAGAAGGAAGCGCTGAAGAATCTAGAAGATGTGCTTCTAGAACCCCCGAGAGCCTCGTCTGGGAAATCGAGGCGGTACTTGAAGCGAACGAGGTACCTTCCGGACTTGATGGGTGACTCACTCGAGAGCTACCCTAGGAGGGTTTTTATTGATGTAGGGTTGCCGGAGAAGGAAGGCAGAAGTGGGACGAGCTGGTTCATGAAGAACTACCCTACCCGGAACAAGAACTTTGAGATGTACAAGATTGAGACTGTGACAGAGGAGAGTTCCGGGAAGGAGGTGGTTCAAACCGGAATGTCGGAGTGGCTGAACAAGAATGTTAAGGAGGAGGAGTACGTTGTGATGAAGGCTGAGGCCGAGGTGGTGGAGGACATGGTAAAGAGTAAAGCGATTCACTTGGTGGATGAGCTTTTCTTGGAGTGCAAGCCACAGGGACATAATGGAAAAATTAATGTTAGGAGCCGAAGGGCATATTGGCAGTGCTTGGCATTGTACGGACAGCTTCGGGACGAAGGTGTTGCGGTGCATCAATGGTGGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

413

Amino Acids

46.88

Weight (kDa)

9.57

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 237 - 411 6.3e-85 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 237
Acc65I GGTACC 1 cut(s) 789
AccB1I GGYRCC 1 cut(s) 789
AccI GTMKAC 1 cut(s) 576
AccII CGCG 1 cut(s) 172
AccIII TCCGGA 2 cut(s) 103, 796
AciI CCGC 6 cut(s) 170, 172, 330, 468, 769, 1217
AclWI GGATC 1 cut(s) 115
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 1 cut(s) 99
AcuI CTGAAG 3 cut(s) 266, 503, 731
AcyI GRCGYC 1 cut(s) 234
AdeI CACNNNGTG 1 cut(s) 1096
AfaI GTAC 7 cut(s) 31, 86, 773, 791, 935, 1031, 1190
AfeI AGCGCT 1 cut(s) 708
AfiI CCNNNNNNNGG 3 cut(s) 274, 756, 835
AgsI TTSAA 5 cut(s) 178, 485, 550, 778, 983
AjuI GAANNNNNNNTTGG 2 cut(s) 1153, 1185
Alw26I GTCTC 3 cut(s) 327, 457, 938
AlwI GGATC 1 cut(s) 115
Ama87I CYCGRG 2 cut(s) 742, 818
Aor13HI TCCGGA 2 cut(s) 103, 796
Aor51HI AGCGCT 1 cut(s) 708
AoxI GGCC 3 cut(s) 276, 286, 1050
ApeKI GCWGC 2 cut(s) 53, 327
ApoI RAATTY 1 cut(s) 99
ArsI GACNNNNNNTTYG 4 cut(s) 330, 362, 431, 463
AseI ATTAAT 2 cut(s) 381, 1146
Asp718I GGTACC 1 cut(s) 789
AspA2I CCTAGG 1 cut(s) 830
AspLEI GCGC 1 cut(s) 709
AspS9I GGNCC 1 cut(s) 16
AsuC2I CCSGG 2 cut(s) 913, 967
AsuHPI GGTGA 1 cut(s) 821
AsuII TTCGAA 1 cut(s) 97
AvaI CYCGRG 2 cut(s) 742, 818
AvaII GGWCC 1 cut(s) 16
AvrII CCTAGG 1 cut(s) 830
AxyI CCTNAGG 1 cut(s) 581
BalI TGGCCA 1 cut(s) 278
BanI GGYRCC 1 cut(s) 789
BbsI GAAGAC 1 cut(s) 623
BbvCI CCTCAGC 1 cut(s) 1047
BbvI GCAGC 2 cut(s) 40, 339
BccI CCATC 2 cut(s) 565, 799
BcgI CGANNNNNNTGC 2 cut(s) 1196, 1230
BciVI GTATCC 1 cut(s) 81
BcnI CCSGG 2 cut(s) 913, 967
BcoDI GTCTC 3 cut(s) 327, 457, 938
BfaI CTAG 5 cut(s) 111, 687, 719, 734, 831
BfoI RGCGCY 1 cut(s) 710
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 3 cut(s) 54, 328, 468
BlnI CCTAGG 1 cut(s) 830
BlpI GCTNAGC 1 cut(s) 524
BlsI GCNGC 3 cut(s) 55, 329, 469
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 2 cut(s) 913, 967
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 2 cut(s) 742, 818
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 2 cut(s) 791, 1157
BmrFI CCNGG 2 cut(s) 913, 967
BmsI GCATC 4 cut(s) 67, 165, 362, 1231
BpiI GAAGAC 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 1047
Bpu1102I GCTNAGC 1 cut(s) 524
Bpu14I TTCGAA 1 cut(s) 97
BpuMI CCSGG 2 cut(s) 913, 967
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 4 cut(s) 19, 170, 830, 1053
BsaWI WCCGGW 3 cut(s) 103, 796, 986
BsaXI ACNNNNNCTCC 4 cut(s) 646, 676, 1013, 1043
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 756, 835
Bse21I CCTNAGG 1 cut(s) 581
Bse3DI GCAATG 2 cut(s) 535, 615
BseAI TCCGGA 2 cut(s) 103, 796
BseDI CCNNGG 4 cut(s) 19, 170, 830, 1053
BseGI GGATG 2 cut(s) 227, 1105
BseLI CCNNNNNNNGG 3 cut(s) 274, 756, 835
BseMI GCAATG 2 cut(s) 535, 615
BseMII CTCAG 5 cut(s) 371, 432, 538, 572, 1038
BseRI GAGGAG 3 cut(s) 971, 1037, 1040
BseXI GCAGC 2 cut(s) 40, 339
Bsh1236I CGCG 1 cut(s) 172
BshFI GGCC 3 cut(s) 278, 288, 1052
BshNI GGYRCC 1 cut(s) 789
BsiHKCI CYCGRG 2 cut(s) 742, 818
BsiSI CCGG 6 cut(s) 104, 797, 860, 913, 966, 987
BslFI GGGAC 4 cut(s) 407, 895, 1143, 1217
BslI CCNNNNNNNGG 3 cut(s) 274, 756, 835
BsmAI GTCTC 3 cut(s) 327, 457, 938
BsmBI CGTCTC 1 cut(s) 457
BsmFI GGGAC 4 cut(s) 407, 895, 1143, 1217
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 3 cut(s) 278, 288, 1052
BsoBI CYCGRG 2 cut(s) 742, 818
Bsp119I TTCGAA 1 cut(s) 97
Bsp13I TCCGGA 2 cut(s) 103, 796
Bsp1407I TGTACA 1 cut(s) 933
Bsp143I GATC 4 cut(s) 6, 120, 261, 387
Bsp1720I GCTNAGC 1 cut(s) 524
BspACI CCGC 6 cut(s) 170, 172, 330, 468, 769, 1217
BspANI GGCC 3 cut(s) 278, 288, 1052
BspCNI CTCAG 5 cut(s) 370, 431, 537, 573, 1039
BspEI TCCGGA 2 cut(s) 103, 796
BspFNI CGCG 1 cut(s) 172
BspHI TCATGA 1 cut(s) 894
BspLI GGNNCC 2 cut(s) 791, 1157
BspPI GGATC 1 cut(s) 115
BspT104I TTCGAA 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 789
BsrDI GCAATG 2 cut(s) 535, 615
BsrGI TGTACA 1 cut(s) 933
BssECI CCNNGG 4 cut(s) 19, 170, 830, 1053
BssMI GATC 4 cut(s) 6, 120, 261, 387
BssNAI GTATAC 1 cut(s) 577
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 2 cut(s) 19, 830
Bst1107I GTATAC 1 cut(s) 577
Bst4CI ACNGT 1 cut(s) 949
BstACI GRCGYC 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 933
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 634, 1121
BstDEI CTNAG 5 cut(s) 357, 418, 524, 581, 1047
BstDSI CCRYGG 1 cut(s) 170
BstF5I GGATG 2 cut(s) 227, 1105
BstFNI CGCG 1 cut(s) 172
BstH2I RGCGCY 1 cut(s) 710
BstHHI GCGC 1 cut(s) 709
BstKTI GATC 4 cut(s) 9, 123, 264, 390
BstMAI GTCTC 3 cut(s) 327, 457, 938
BstMBI GATC 4 cut(s) 6, 120, 261, 387
BstMWI GCNNNNNNNGC 3 cut(s) 350, 464, 692
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 2 cut(s) 911, 965
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 40, 339
BstV2I GAAGAC 1 cut(s) 623
BstXI CCANNNNNNTGG 1 cut(s) 88
BstZ17I GTATAC 1 cut(s) 577
Bsu36I CCTNAGG 1 cut(s) 581
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 3 cut(s) 278, 288, 1052
BtgI CCRYGG 1 cut(s) 170
BtsCI GGATG 2 cut(s) 227, 1105
BtsI GCAGTG 1 cut(s) 1181
BtsIMutI CAGTG 1 cut(s) 1181
Cac8I GCNNGC 2 cut(s) 634, 1121
CciI TCATGA 1 cut(s) 894
CfoI GCGC 1 cut(s) 709
Cfr13I GGNCC 1 cut(s) 16
Cfr42I CCGCGG 1 cut(s) 173
CseI GACGC 1 cut(s) 542
Csp6I GTAC 7 cut(s) 30, 85, 772, 790, 934, 1030, 1189
CviAII CATG 3 cut(s) 409, 895, 1069
CviQI GTAC 7 cut(s) 30, 85, 772, 790, 934, 1030, 1189
DdeI CTNAG 5 cut(s) 357, 418, 524, 581, 1047
DpnI GATC 4 cut(s) 8, 122, 263, 389
DpnII GATC 4 cut(s) 6, 120, 261, 387
DraIII CACNNNGTG 1 cut(s) 1096
EaeI YGGCCR 1 cut(s) 276
Eco130I CCWWGG 2 cut(s) 19, 830
Eco147I AGGCCT 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 16
Eco47III AGCGCT 1 cut(s) 708
Eco57I CTGAAG 3 cut(s) 266, 503, 731
Eco81I CCTNAGG 1 cut(s) 581
Eco88I CYCGRG 2 cut(s) 742, 818
EcoRI GAATTC 1 cut(s) 99
EcoT14I CCWWGG 2 cut(s) 19, 830
ErhI CCWWGG 2 cut(s) 19, 830
Esp3I CGTCTC 1 cut(s) 457
FaeI CATG 3 cut(s) 412, 898, 1072
FalI AAGNNNNNCTT 4 cut(s) 625, 657, 714, 746
FaqI GGGAC 4 cut(s) 407, 895, 1143, 1217
FatI CATG 3 cut(s) 408, 894, 1068
FauI CCCGC 1 cut(s) 177
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 3 cut(s) 54, 328, 468
FokI GGATG 2 cut(s) 214, 1112
Fsp4HI GCNGC 3 cut(s) 54, 328, 468
FspBI CTAG 5 cut(s) 111, 687, 719, 734, 831
GlaI GCGC 1 cut(s) 708
GluI GCNGC 3 cut(s) 54, 328, 468
HaeII RGCGCY 1 cut(s) 710
HaeIII GGCC 3 cut(s) 278, 288, 1052
HapII CCGG 6 cut(s) 104, 797, 860, 913, 966, 987
HgaI GACGC 1 cut(s) 542
HhaI GCGC 1 cut(s) 709
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 3 cut(s) 412, 898, 1072
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HinfI GANTC 5 cut(s) 107, 490, 715, 812, 1087
HpaII CCGG 6 cut(s) 104, 797, 860, 913, 966, 987
HphI GGTGA 1 cut(s) 821
Hpy166II GTNNAC 1 cut(s) 577
Hpy188I TCNGA 5 cut(s) 65, 232, 246, 406, 997
Hpy8I GTNNAC 1 cut(s) 577
Hpy99I CGWCG 3 cut(s) 131, 236, 239
HpyCH4III ACNGT 1 cut(s) 949
HpyCH4IV ACGT 2 cut(s) 234, 1032
HpyCH4V TGCA 7 cut(s) 156, 412, 446, 608, 632, 1119, 1222
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 464, 692
HpyF3I CTNAG 5 cut(s) 357, 418, 524, 581, 1047
HpySE526I ACGT 2 cut(s) 234, 1032
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 3 cut(s) 412, 898, 1072
HspAI GCGC 1 cut(s) 707
Kpn2I TCCGGA 2 cut(s) 103, 796
KpnI GGTACC 1 cut(s) 793
KspI CCGCGG 1 cut(s) 173
Kzo9I GATC 4 cut(s) 6, 120, 261, 387
LmnI GCTCC 4 cut(s) 61, 171, 255, 1155
Lsp1109I GCAGC 2 cut(s) 40, 339
LweI GCATC 4 cut(s) 67, 165, 362, 1231
MaeI CTAG 5 cut(s) 111, 687, 719, 734, 831
MaeII ACGT 2 cut(s) 234, 1032
MaeIII GTNAC 3 cut(s) 269, 809, 949
MalI GATC 4 cut(s) 8, 122, 263, 389
MboI GATC 4 cut(s) 6, 120, 261, 387
MboII GAAGA 6 cut(s) 256, 538, 628, 724, 734, 910
MlsI TGGCCA 1 cut(s) 278
MluCI AATT 5 cut(s) 10, 99, 219, 498, 1143
MluNI TGGCCA 1 cut(s) 278
MlyI GAGTC 2 cut(s) 484, 806
MmeI TCCRAC 4 cut(s) 255, 429, 564, 975
Mox20I TGGCCA 1 cut(s) 278
MroI TCCGGA 2 cut(s) 103, 796
MscI TGGCCA 1 cut(s) 278
MseI TTAA 6 cut(s) 140, 240, 381, 495, 1017, 1146
Msp20I TGGCCA 1 cut(s) 278
MspA1I CMGCKG 2 cut(s) 172, 330
MspI CCGG 6 cut(s) 104, 797, 860, 913, 966, 987
MspR9I CCNGG 2 cut(s) 913, 967
Mva1269I GAATGC 1 cut(s) 446
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 3 cut(s) 350, 464, 692
NciI CCSGG 2 cut(s) 913, 967
NdeII GATC 4 cut(s) 6, 120, 261, 387
NlaIII CATG 3 cut(s) 412, 898, 1072
NlaIV GGNNCC 2 cut(s) 791, 1157
NmeAIII GCCGAG 1 cut(s) 1078
NmuCI GTSAC 3 cut(s) 269, 809, 949
NspI RCATGY 1 cut(s) 412
NspV TTCGAA 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 818
PagI TCATGA 1 cut(s) 894
PceI AGGCCT 1 cut(s) 288
PctI GAATGC 1 cut(s) 446
PfeI GAWTC 3 cut(s) 107, 715, 1087
PfoI TCCNGGA 1 cut(s) 965
PkrI GCNGC 3 cut(s) 55, 329, 469
PleI GAGTC 2 cut(s) 484, 806
PpsI GAGTC 2 cut(s) 484, 806
PshBI ATTAAT 2 cut(s) 381, 1146
PspN4I GGNNCC 2 cut(s) 791, 1157
PspPI GGNCC 1 cut(s) 16
RsaI GTAC 7 cut(s) 31, 86, 773, 791, 935, 1031, 1190
RsaNI GTAC 7 cut(s) 30, 85, 772, 790, 934, 1030, 1189
SacII CCGCGG 1 cut(s) 173
SaqAI TTAA 6 cut(s) 140, 240, 381, 495, 1017, 1146
SatI GCNGC 3 cut(s) 54, 328, 468
Sau3AI GATC 4 cut(s) 6, 120, 261, 387
Sau96I GGNCC 1 cut(s) 16
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 2 cut(s) 484, 806
ScrFI CCNGG 2 cut(s) 913, 967
SfaNI GCATC 4 cut(s) 67, 165, 362, 1231
Sfr274I CTCGAG 1 cut(s) 818
Sfr303I CCGCGG 1 cut(s) 173
SfuI TTCGAA 1 cut(s) 97
SgrBI CCGCGG 1 cut(s) 173
SinI GGWCC 1 cut(s) 16
SlaI CTCGAG 1 cut(s) 818
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
Sse9I AATT 5 cut(s) 10, 99, 219, 498, 1143
SseBI AGGCCT 1 cut(s) 288
SsiI CCGC 6 cut(s) 170, 172, 330, 468, 769, 1217
SspMI CTAG 5 cut(s) 111, 687, 719, 734, 831
StuI AGGCCT 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 911, 965
StyI CCWWGG 2 cut(s) 19, 830
TaaI ACNGT 1 cut(s) 949
TaiI ACGT 2 cut(s) 237, 1035
TaqI TCGA 5 cut(s) 97, 390, 438, 764, 819
TasI AATT 5 cut(s) 10, 99, 219, 498, 1143
TatI WGTACW 2 cut(s) 84, 933
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 3 cut(s) 107, 715, 1087
Tru1I TTAA 6 cut(s) 140, 240, 381, 495, 1017, 1146
Tru9I TTAA 6 cut(s) 140, 240, 381, 495, 1017, 1146
TscAI CASTG 1 cut(s) 1181
TseFI GTSAC 3 cut(s) 269, 809, 949
TseI GCWGC 2 cut(s) 53, 327
Tsp45I GTSAC 3 cut(s) 269, 809, 949
TspDTI ATGAA 4 cut(s) 469, 883, 911, 1055
TspGWI ACGGA 3 cut(s) 17, 257, 1206
TspRI CASTG 1 cut(s) 1181
VpaK11BI GGWCC 1 cut(s) 16
VspI ATTAAT 2 cut(s) 381, 1146
XapI RAATTY 1 cut(s) 99
XbaI TCTAGA 3 cut(s) 110, 718, 733
XceI RCATGY 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 818
XmaJI CCTAGG 1 cut(s) 830
XmiI GTMKAC 1 cut(s) 576
XspI CTAG 5 cut(s) 111, 687, 719, 734, 831
ZraI GACGTC 1 cut(s) 235
ZrmI AGTACT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.