FvH4_5g37670

methyltransferase At1g78140, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007867
Physical Location & Seq
Forward (+)
27722920 .. 27727256
4337 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g37670.t1

Sequence Viewer

Length: 1038 bp
ATGGCGACGACGAAGCTCACCAGCAACCTCTCTCTCCCGAGTCAACTCGGCAACTCGAGGCCTGACTCGTTCAAGCTCAGCAACCCATTCACCGTTAGCTTCAAACGCTCTTCTTTCCCGACAATCACTGTCCGAGCTTCTTCCACCGCCTTTGTCGAGACCACCCCATCTGAACCGATTGAGGTTCAGAATGAGGCCACCATCTGTAACGATTTGCTGGCTTGTCCTATATGCTTTGATCGATTTTCATCAAAAGGCGAGTCTGGTTCCGGATCTAGTTTCGAATGTAGCACTTGTAAGAAGACTTACTCTAGCAATGAGACACATTTGGACTTGACACTGGCGAGCGGTGCCAAGAACTATGGTGAATCAATGCCGGCTTCCACGGAGTTTTTCAGGATTCCGTTGATATCTTTCCTTTACGAGAGGGGCTGGCGTCAAAGCTTTTCAGTATGGGGTGGTTTTCCTGGGCCAGAGAAAGAGTTTGAGTTGATTAAGGATTGCATAAAACCAGTCCTGGGTGGAAGCATCATTGATGCTAGCTGTGGGAGTGGGTTATTTTCCAGGCTTTTCGCCAAGAGTGGATTGTTTTCTCATGTTGTTGCTCTGGACTACTCAGAAAATATGTTGAAGCAGTGCTATGAGTTCATTGAGAAGGAAGAGAACTTCCCCAAAGAGAACATAACTTTGGTCAGAGCTGATATTTCTAGGCTTCCCTTTGCTACAAGTTCTATTGATGCTGTGCATGCTGGTGCTGCCATACACTGTTGGCCTTCACCAACAGGAGCTGTTGCTGAAATTAGCAGAGTACTGCGACCTGGTGGGGTGTTCGTTGCAACAACTTACATACTTGATGGTCCTTTTGCCTTTGTCCCATTTTTGAGGGAAGGAACCAAGAGAGCAAGGCAAGTATCAGGCAGCCAAATCTTCCTATCTGAAGGTGAATTGGAAGACATCTGTACAGCATGTGGCCTTGTAGGTGTTACTGTTGTGAGGAATCGGCAATTCGTGATGATCTCTGCTACAAAACCAAGTTAA

Protein Analysis

346

Amino Acids

37.55

Weight (kDa)

6.16

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 165 - 279 8.3e-11 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 173 - 284 4.3e-08 Methyltransferase domain
Methyltransf_31 PF13847 175 - 283 5.1e-16 Methyltransferase domain
Methyltransf_25 PF13649 177 - 275 1.2e-18 Methyltransferase domain
Methyltransf_11 PF08241 179 - 278 1.9e-22 Methyltransferase domain
Methyltransf_12 PF08242 179 - 277 8.4e-13 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 350
AccBSI CCGCTC 1 cut(s) 348
AccIII TCCGGA 1 cut(s) 269
AciI CCGC 2 cut(s) 147, 348
AclWI GGATC 1 cut(s) 280
AcuI CTGAAG 1 cut(s) 957
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 2 cut(s) 810, 961
AfiI CCNNNNNNNGG 1 cut(s) 518
AgsI TTSAA 3 cut(s) 73, 103, 631
AjnI CCWGG 4 cut(s) 466, 516, 563, 817
AjuI GAANNNNNNNTTGG 2 cut(s) 671, 703
AluBI AGCT 8 cut(s) 16, 76, 99, 137, 444, 543, 698, 788
AluI AGCT 8 cut(s) 16, 76, 99, 137, 444, 543, 698, 788
Alw26I GTCTC 2 cut(s) 152, 314
AlwI GGATC 1 cut(s) 280
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 2 cut(s) 37, 55
Aor13HI TCCGGA 1 cut(s) 269
AoxI GGCC 5 cut(s) 59, 195, 470, 770, 970
ApeKI GCWGC 2 cut(s) 755, 918
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
AspS9I GGNCC 2 cut(s) 470, 857
AsuHPI GGTGA 5 cut(s) 10, 82, 377, 768, 953
AsuII TTCGAA 1 cut(s) 282
AsuNHI GCTAGC 1 cut(s) 539
AvaI CYCGRG 2 cut(s) 37, 55
AvaII GGWCC 1 cut(s) 857
BanI GGYRCC 1 cut(s) 350
BbsI GAAGAC 2 cut(s) 308, 957
BbvI GCAGC 2 cut(s) 742, 930
BccI CCATC 3 cut(s) 175, 209, 848
BciT130I CCWGG 4 cut(s) 468, 518, 565, 819
BcoDI GTCTC 2 cut(s) 152, 314
BfaI CTAG 4 cut(s) 276, 312, 540, 708
BisI GCNGC 2 cut(s) 756, 919
BlpI GCTNAGC 1 cut(s) 77
BlsI GCNGC 2 cut(s) 757, 920
BmcAI AGTACT 1 cut(s) 810
Bme1390I CCNGG 4 cut(s) 468, 518, 565, 819
Bme18I GGWCC 1 cut(s) 857
BmeT110I CYCGRG 2 cut(s) 37, 55
BmgT120I GGNCC 2 cut(s) 470, 857
BmiI GGNNCC 3 cut(s) 268, 352, 892
BmrFI CCNGG 4 cut(s) 468, 518, 565, 819
BmsI GCATC 3 cut(s) 526, 537, 727
BmtI GCTAGC 1 cut(s) 543
BpiI GAAGAC 2 cut(s) 308, 957
Bpu1102I GCTNAGC 1 cut(s) 77
Bpu14I TTCGAA 1 cut(s) 282
Bsa29I ATCGAT 1 cut(s) 241
BsaBI GATNNNNATC 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 436
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 384, 467, 517
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 1 cut(s) 518
Bse118I RCCGGY 1 cut(s) 376
Bse1I ACTGG 2 cut(s) 345, 512
Bse3DI GCAATG 1 cut(s) 322
Bse8I GATNNNNATC 1 cut(s) 247
BseAI TCCGGA 1 cut(s) 269
BseBI CCWGG 4 cut(s) 468, 518, 565, 819
BseCI ATCGAT 1 cut(s) 241
BseDI CCNNGG 3 cut(s) 384, 467, 517
BseJI GATNNNNATC 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 518
BseMI GCAATG 1 cut(s) 322
BseMII CTCAG 2 cut(s) 91, 630
BseNI ACTGG 2 cut(s) 345, 512
BseXI GCAGC 2 cut(s) 742, 930
BshFI GGCC 5 cut(s) 61, 197, 472, 772, 972
BshNI GGYRCC 1 cut(s) 350
BshVI ATCGAT 1 cut(s) 241
BsiHKCI CYCGRG 2 cut(s) 37, 55
BsiSI CCGG 2 cut(s) 270, 377
BslFI GGGAC 1 cut(s) 857
BslI CCNNNNNNNGG 1 cut(s) 518
BsmAI GTCTC 2 cut(s) 152, 314
BsmFI GGGAC 1 cut(s) 857
BsnI GGCC 5 cut(s) 61, 197, 472, 772, 972
Bso31I GGTCTC 1 cut(s) 152
BsoBI CYCGRG 2 cut(s) 37, 55
Bsp119I TTCGAA 1 cut(s) 282
Bsp13I TCCGGA 1 cut(s) 269
Bsp1407I TGTACA 1 cut(s) 959
Bsp143I GATC 3 cut(s) 238, 272, 1014
Bsp1720I GCTNAGC 1 cut(s) 77
BspACI CCGC 2 cut(s) 147, 348
BspANI GGCC 5 cut(s) 61, 197, 472, 772, 972
BspCNI CTCAG 2 cut(s) 90, 629
BspDI ATCGAT 1 cut(s) 241
BspEI TCCGGA 1 cut(s) 269
BspLI GGNNCC 3 cut(s) 268, 352, 892
BspOI GCTAGC 1 cut(s) 543
BspPI GGATC 1 cut(s) 280
BspQI GCTCTTC 1 cut(s) 115
BspT104I TTCGAA 1 cut(s) 282
BspT107I GGYRCC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 152
BsrBI CCGCTC 1 cut(s) 348
BsrDI GCAATG 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 376
BsrGI TGTACA 1 cut(s) 959
BsrI ACTGG 2 cut(s) 345, 512
BssAI RCCGGY 1 cut(s) 376
BssECI CCNNGG 3 cut(s) 384, 467, 517
BssMI GATC 3 cut(s) 238, 272, 1014
BssNI GRCGYC 1 cut(s) 436
Bst2UI CCWGG 4 cut(s) 468, 518, 565, 819
Bst4CI ACNGT 4 cut(s) 94, 130, 767, 988
Bst6I CTCTTC 2 cut(s) 115, 654
BstACI GRCGYC 1 cut(s) 436
BstAUI TGTACA 1 cut(s) 959
BstBI TTCGAA 1 cut(s) 282
BstC8I GCNNGC 6 cut(s) 219, 346, 378, 434, 541, 747
BstDEI CTNAG 2 cut(s) 77, 616
BstDSI CCRYGG 1 cut(s) 384
BstKTI GATC 3 cut(s) 241, 275, 1017
BstMAI GTCTC 2 cut(s) 152, 314
BstMBI GATC 3 cut(s) 238, 272, 1014
BstMWI GCNNNNNNNGC 4 cut(s) 105, 350, 746, 755
BstNI CCWGG 4 cut(s) 468, 518, 565, 819
BstNSI RCATGY 2 cut(s) 749, 969
BstSCI CCNGG 4 cut(s) 466, 516, 563, 817
BstV1I GCAGC 2 cut(s) 742, 930
BstV2I GAAGAC 2 cut(s) 308, 957
BstX2I RGATCY 1 cut(s) 272
BstYI RGATCY 1 cut(s) 272
Bsu15I ATCGAT 1 cut(s) 241
BsuRI GGCC 5 cut(s) 61, 197, 472, 772, 972
BsuTUI ATCGAT 1 cut(s) 241
BtgI CCRYGG 1 cut(s) 384
BtsI GCAGTG 1 cut(s) 641
BtsIMutI CAGTG 4 cut(s) 126, 338, 641, 763
Cac8I GCNNGC 6 cut(s) 219, 346, 378, 434, 541, 747
CaiI CAGNNNCTG 1 cut(s) 788
Cfr10I RCCGGY 1 cut(s) 376
Cfr13I GGNCC 2 cut(s) 470, 857
ClaI ATCGAT 1 cut(s) 241
CseI GACGC 1 cut(s) 425
CsiI ACCWGGT 1 cut(s) 817
Csp6I GTAC 2 cut(s) 809, 960
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviAII CATG 3 cut(s) 596, 746, 966
CviQI GTAC 2 cut(s) 809, 960
DdeI CTNAG 2 cut(s) 77, 616
DpnI GATC 3 cut(s) 240, 274, 1016
DpnII GATC 3 cut(s) 238, 272, 1014
Eam1104I CTCTTC 2 cut(s) 115, 654
EarI CTCTTC 2 cut(s) 115, 654
Eco147I AGGCCT 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 152
Eco32I GATATC 1 cut(s) 411
Eco47I GGWCC 1 cut(s) 857
Eco57I CTGAAG 1 cut(s) 957
Eco88I CYCGRG 2 cut(s) 37, 55
EcoRII CCWGG 4 cut(s) 466, 516, 563, 817
EcoRV GATATC 1 cut(s) 411
FaeI CATG 3 cut(s) 599, 749, 969
FaqI GGGAC 1 cut(s) 857
FatI CATG 3 cut(s) 595, 745, 965
Fnu4HI GCNGC 2 cut(s) 756, 919
Fsp4HI GCNGC 2 cut(s) 756, 919
FspBI CTAG 4 cut(s) 276, 312, 540, 708
GluI GCNGC 2 cut(s) 756, 919
HaeIII GGCC 5 cut(s) 61, 197, 472, 772, 972
HapII CCGG 2 cut(s) 270, 377
HgaI GACGC 1 cut(s) 425
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 3 cut(s) 599, 749, 969
HincII GTYRAC 1 cut(s) 44
HindII GTYRAC 1 cut(s) 44
HindIII AAGCTT 1 cut(s) 442
HinfI GANTC 6 cut(s) 40, 65, 260, 368, 400, 997
HpaII CCGG 2 cut(s) 270, 377
HphI GGTGA 5 cut(s) 10, 82, 377, 768, 953
Hpy166II GTNNAC 1 cut(s) 44
Hpy188I TCNGA 6 cut(s) 134, 172, 189, 619, 695, 937
Hpy188III TCNNGA 7 cut(s) 37, 118, 157, 270, 397, 608, 1009
Hpy8I GTNNAC 1 cut(s) 44
Hpy99I CGWCG 2 cut(s) 10, 13
HpyAV CCTTC 4 cut(s) 649, 783, 881, 932
HpyCH4III ACNGT 4 cut(s) 94, 130, 767, 988
HpyCH4V TGCA 3 cut(s) 504, 745, 836
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 350, 746, 755
HpyF3I CTNAG 2 cut(s) 77, 616
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 3 cut(s) 599, 749, 969
Kpn2I TCCGGA 1 cut(s) 269
KroI GCCGGC 1 cut(s) 376
KroNI GCCGGC 1 cut(s) 378
Kzo9I GATC 3 cut(s) 238, 272, 1014
LguI GCTCTTC 1 cut(s) 115
LmnI GCTCC 1 cut(s) 785
Lsp1109I GCAGC 2 cut(s) 742, 930
LweI GCATC 3 cut(s) 526, 537, 727
MabI ACCWGGT 1 cut(s) 817
MaeI CTAG 4 cut(s) 276, 312, 540, 708
MaeIII GTNAC 2 cut(s) 206, 982
MalI GATC 3 cut(s) 240, 274, 1016
MbiI CCGCTC 1 cut(s) 348
MboI GATC 3 cut(s) 238, 272, 1014
MboII GAAGA 6 cut(s) 102, 132, 313, 671, 919, 962
MflI RGATCY 1 cut(s) 272
MluCI AATT 3 cut(s) 798, 944, 1004
MlyI GAGTC 3 cut(s) 49, 59, 269
MnlI CCTC 7 cut(s) 38, 51, 175, 187, 420, 876, 987
MroI TCCGGA 1 cut(s) 269
MroNI GCCGGC 1 cut(s) 376
MseI TTAA 2 cut(s) 495, 1036
MslI CAYNNNNRTG 1 cut(s) 750
MspI CCGG 2 cut(s) 270, 377
MspR9I CCNGG 4 cut(s) 468, 518, 565, 819
MvaI CCWGG 4 cut(s) 468, 518, 565, 819
MwoI GCNNNNNNNGC 4 cut(s) 105, 350, 746, 755
NaeI GCCGGC 1 cut(s) 378
NdeII GATC 3 cut(s) 238, 272, 1014
NgoMIV GCCGGC 1 cut(s) 376
NheI GCTAGC 1 cut(s) 539
NlaIII CATG 3 cut(s) 599, 749, 969
NlaIV GGNNCC 3 cut(s) 268, 352, 892
NmeAIII GCCGAG 1 cut(s) 27
NspI RCATGY 2 cut(s) 749, 969
NspV TTCGAA 1 cut(s) 282
PaeI GCATGC 1 cut(s) 749
PaeR7I CTCGAG 1 cut(s) 55
PceI AGGCCT 1 cut(s) 61
PciSI GCTCTTC 1 cut(s) 115
PdiI GCCGGC 1 cut(s) 378
PfeI GAWTC 3 cut(s) 368, 400, 997
PkrI GCNGC 2 cut(s) 757, 920
PleI GAGTC 3 cut(s) 48, 59, 268
PpsI GAGTC 3 cut(s) 48, 59, 268
Psp6I CCWGG 4 cut(s) 466, 516, 563, 817
PspGI CCWGG 4 cut(s) 466, 516, 563, 817
PspN4I GGNNCC 3 cut(s) 268, 352, 892
PspPI GGNCC 2 cut(s) 470, 857
PspXI VCTCGAGB 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 1 cut(s) 272
RsaI GTAC 2 cut(s) 810, 961
RsaNI GTAC 2 cut(s) 809, 960
RseI CAYNNNNRTG 1 cut(s) 750
SapI GCTCTTC 1 cut(s) 115
SaqAI TTAA 2 cut(s) 495, 1036
SatI GCNGC 2 cut(s) 756, 919
Sau3AI GATC 3 cut(s) 238, 272, 1014
Sau96I GGNCC 2 cut(s) 470, 857
ScaI AGTACT 1 cut(s) 810
SchI GAGTC 3 cut(s) 49, 59, 269
ScrFI CCNGG 4 cut(s) 468, 518, 565, 819
SexAI ACCWGGT 1 cut(s) 817
SfaNI GCATC 3 cut(s) 526, 537, 727
Sfr274I CTCGAG 1 cut(s) 55
SfuI TTCGAA 1 cut(s) 282
SinI GGWCC 1 cut(s) 857
SlaI CTCGAG 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 750
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
SphI GCATGC 1 cut(s) 749
Sse9I AATT 3 cut(s) 798, 944, 1004
SseBI AGGCCT 1 cut(s) 61
SsiI CCGC 2 cut(s) 147, 348
SspMI CTAG 4 cut(s) 276, 312, 540, 708
StuI AGGCCT 1 cut(s) 61
StyD4I CCNGG 4 cut(s) 466, 516, 563, 817
TaaI ACNGT 4 cut(s) 94, 130, 767, 988
TaqI TCGA 4 cut(s) 56, 156, 241, 282
TasI AATT 3 cut(s) 798, 944, 1004
TatI WGTACW 2 cut(s) 808, 959
TfiI GAWTC 3 cut(s) 368, 400, 997
Tru1I TTAA 2 cut(s) 495, 1036
Tru9I TTAA 2 cut(s) 495, 1036
TscAI CASTG 4 cut(s) 133, 345, 641, 770
TseI GCWGC 2 cut(s) 755, 918
TspDTI ATGAA 2 cut(s) 237, 637
TspGWI ACGGA 2 cut(s) 393, 401
TspRI CASTG 4 cut(s) 133, 345, 641, 770
VpaK11BI GGWCC 1 cut(s) 857
XceI RCATGY 2 cut(s) 749, 969
XhoI CTCGAG 1 cut(s) 55
XspI CTAG 4 cut(s) 276, 312, 540, 708
ZrmI AGTACT 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.